CN102628874A - Full-automatic colloidal gold detection method and full-automatic colloidal gold detection instrument - Google Patents
Full-automatic colloidal gold detection method and full-automatic colloidal gold detection instrument Download PDFInfo
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- CN102628874A CN102628874A CN2012101326926A CN201210132692A CN102628874A CN 102628874 A CN102628874 A CN 102628874A CN 2012101326926 A CN2012101326926 A CN 2012101326926A CN 201210132692 A CN201210132692 A CN 201210132692A CN 102628874 A CN102628874 A CN 102628874A
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Abstract
The invention provides a full-automatic colloidal gold detection instrument which comprises a controller, a detection card conveying platform, a constant-temperature incubator, a cassette, a CCD (Charge Coupled Device) image collector, an image collector positioning guide rail and a waste card collection box, wherein the detection card conveying platform adopts a conveying belt or a conveying disc, the conveying belt or the conveying disc is driven by a servo motor or a stepping motor; detection cards are sequentially placed at conveying positions of the conveying belt or the conveying disc; an image collector is arranged above one region of the conveying belt or the conveying disc and is positioned on the guide rail; a detection card accommodating region is arranged at the input part of the conveying belt or the conveying disc, and the constant-temperature incubator is behind the detection card accommodating region. According to the full-automatic colloidal gold detection instrument, a continuous sampling device is additionally arranged, so that the detection speed is greatly improved; and a positioning track system of the image collector is additionally arranged, so that the collection position of the image collector is optimized, the reaction speed is quickened, the detection process is reasonable and reliable, the test result is enabled to be accurate.
Description
Technical field
The present invention relates to the automatic testing method and the device of Biosample, especially collaurum detection method and test card arrangement for reading.
Background technology
The collaurum test card is that specific antigen or antibody (detect and use reagent) are fixed on the film with ribbon (coating); Colloid gold label reagent (antibody or monoclonal antibody) is adsorbed on the pad; After sample to be checked is added on the sample pad of test strips one end in the test card; Move forward through capillary action; React to each other behind the colloid gold label reagent of dissolving on the pad, when moving to fixing antigen or antibody regional again, specificity takes place again with it and combines and be trapped in thing to be checked and the bond of gold marked reagent; Accumulate in to detect and be with, whether can develop the color through being observed visually (perhaps according to colour developing degree and colour atla contrast) judges testing result.
Existing collaurum test card reader; Existing reader mainly is to be provided with a CCD (perhaps CMOS) image acquisition device a magazine inside color development area of rapid detection card is carried out IMAQ; Through software to the graphical analysis of being gathered with the decision analysis result; It is formed structure and generally is provided with a plug-in card hole (or a plug-type cartridge); The test card that will add sample during use is put into the plug-in card hole, and question response finishes the back automatic images acquired of card reader and calculates test result.
Though 1, existing card reader has solved and had judged the parallax error that produces through naked eyes in the past; But because it once can only place a slice test card; And testing sample joins and needs the certain reaction time after the test card (with the clenobuterol hydrochloride is example; Need reaction three minutes), cause detection speed lower, can not satisfy batch samples the detection demand (at present the slaughterhouse required to each slaughter object all take a sample detect).
2, in addition because the reaction tag line of test card is generally all thinner; The markings position of each batch has differences between batches in process of production; And the CCD of card reader generally fixes, and the site error of tag line will produce certain influence to test result.
Summary of the invention
The present invention seeks to, propose a kind of continuous automatic inspection method and apparatus of Biosample, especially collaurum detection method and test card arrangement for reading.And improve the accuracy that collaurum detects.
Technical scheme of the present invention is: the collaurum detection method, the colloidal gold test card bar code is read, and image is gathered in advance, the markings location, and the fastening position moves to the optimum position, the precise image collection, image storage and analyzing and processing also go out result's report.
In specific words: the automatic transmission platform that is provided with the collaurum test card; The test card that will add reagent is placed on the automatic transmission platform; The every interval of transmission platform certain hour (time can preestablish) moves a position, and test card is hatched the district through constant temperature earlier, carries out IMAQ again; Image acquisition device is adjusted to best image capture position to IMAQ by the displacement regulating system.
Elder generation is through preparatory acquisition zone before the IMAQ; Acquisition zone is confirmed the chromogenic line exact position of test card in advance; Subsequent picture collector C CD (perhaps CMOS) displacement system is adjusted to best image capture position with image acquisition device CCD (perhaps CMOS) automatically; Carry out the analysis image collection, and image is analyzed to judge testing result by software.
Ccd image collector displacement system is adjusted to best image capture position with the ccd image collector automatically; Carry out the analysis image collection; And by software image is analyzed to judge testing result, the test card of gathering image is the most at last delivered to useless card and is collected in the box.
Image acquisition device by the method for displacement regulating system is: image acquisition device is regulated through a location guide rail: hang and be displaced in the cross recess a certain position and make image acquisition device obtain chromogenic line exact position.
Transmission platform continues to move after detecting completion, gathers the test card of image the most at last and delivers in the useless card collection box.
Instrument can be set different parameters according to the kind of test card, like qualitative or quantitative test parameter of translational speed, IMAQ number of times and the chromogenic reaction of reaction time, transmission platform etc.
Automatically transmission platform is travelling belt or transmission dish.Device of the present invention is that full-automatic collaurum detector comprises: controller, test card transmission platform, and the constant temperature incubator, magazine, the ccd image collector, the image acquisition device positioning guide rail can also be provided with useless card in addition and collect box; The test card transmission platform adopts travelling belt or transmits disk, and travelling belt or transmission disk increase employing servomotor or step motor drive.Test card places travelling belt successively or transmits the delivering position of disk; Travelling belt or transmit disk a zone above be provided with image acquisition device, image acquisition device is positioned on the location guide rail; Input place of travelling belt or transmission disk is provided with the test card rest area, is the constant temperature incubator after the test card rest area, is provided with useless card collection box at travelling belt or after transmitting disk image acquisition device zone.Positioning guide rail is provided with cross recess as the guide rail regulating device: hang and be displaced into that a certain position makes image acquisition device obtain chromogenic line exact position in the cross recess.Transmission platform continues to move after detecting completion, gathers the test card of image the most at last and delivers in the useless card collection box.
Apparatus of the present invention can be set different parameters according to the kind of test card, like qualitative or quantitative test parameter of translational speed, IMAQ number of times and the chromogenic reaction of reaction time, transmission platform etc.
Beneficial effect of the present invention is: the inventive method and detector increase the continuous sample introduction device on existing conventional equipment basis, significantly improved detection speed, are user-friendly to.Increased the positioning track system of image acquisition device, confirmed the chromogenic line position, optimized the collection position of image acquisition device, eliminated of the influence of test card chromogenic line error testing result through locating preparatory collection.On transporting rail, be provided with constant temperature and hatch device, fast reaction speed, testing process rationally reach efficient, guarantee the test result accuracy.
Description of drawings
Fig. 1 is a pick-up unit synoptic diagram of the present invention;
Fig. 2 is the partial enlarged drawing of Fig. 1;
Fig. 3 is a system architecture of the present invention;
Fig. 4 is a process flow diagram of the present invention.
Pilot lamp 1, guide rail 2, track drive shaft 3, reference column 4, constant temperature oven 5, ccd image collector 6, test card 7, useless card gatherer 9 among the figure.
Embodiment
The collaurum detection method; Be provided with the automatic transmission platform of collaurum test sample, the test card that will add reagent is placed on the automatic transmission platform, and the every interval of transmission platform certain hour (time can preestablish) moves a position; Test card is hatched the district through constant temperature earlier, carries out IMAQ again; Image acquisition device is adjusted to best image capture position to IMAQ by the displacement regulating system.Acquisition zone is confirmed the chromogenic line exact position of test card in advance; Subsequent picture collector C CD (perhaps CMOS) displacement system is adjusted to best image capture position with image acquisition device CCD (perhaps CMOS) automatically; Carry out the analysis image collection, and image is analyzed to judge testing result by software.
Shown in Figure 3, adopting the embedded computer control system is CPU, directly controls power of motor driving and sensor groups test, card mechanical transmission mechanism, temperature control module electric heating device.
Embedded computer is provided with human-computer interaction device: input (button, touch-screen, bar code scan) man-machine interaction output (liquid crystal display, built-in printer, hummer, prompting LED), external interface: RS-232, USB, network interface.
Flow process of the present invention is following: the test card bar code reads, and image is gathered in advance, the markings location, and the fastening position moves to the optimum position, precise image collection, image storage and analyzing and processing and result's report.
When the test card bar code reads through being provided with the automatic transmission platform of collaurum test sample; The test card that will add reagent is placed on the automatic transmission platform; The every interval of transmission platform certain hour (time can preestablish) moves a position; Test card can be hatched the district through constant temperature earlier, carries out IMAQ again; During IMAQ through image gather in advance, markings location, the fastening position moves to the optimum position and carries out the precise image collection again.Image acquisition device is adjusted to best image capture position to the meticulous collection of image by the displacement regulating system.
Elder generation is through preparatory acquisition zone before the IMAQ; Acquisition zone is confirmed chromogenic line (markings) exact position of test card in advance; Subsequent picture collector C CD (perhaps CMOS) displacement system is adjusted to best image capture position with image acquisition device CCD (perhaps CMOS) automatically; Carry out the analysis image collection, and image is analyzed to judge testing result by software.
Ccd image collector displacement system is adjusted to best image capture position with the ccd image collector automatically; Carry out the analysis image collection; And by software image is analyzed to judge testing result, the test card of gathering image is the most at last delivered to useless card and is collected in the box.
During analyser work, the test card that only needs to have added sample is placed on the transmission platform, and the every interval of transmission platform certain hour (time can preestablish) moves a position; Test card is hatched device through constant temperature earlier; Get into magazine again, the ccd image collector carried out first time image earlier and gathered in advance this moment, through software analysis; Confirm the chromogenic line exact position of test card; Ccd image collector displacement system is adjusted to best image capture position with the ccd image collector automatically subsequently, carries out the analysis image collection, and by software image is analyzed to judge testing result; Transmission platform continues to move after detecting completion, gathers the test card of image the most at last and delivers in the useless card collection box.
The formation of apparatus of the present invention is: pilot lamp 1, guide rail 2, controller adopt little process chip (typical chip); Integrated or establish storer in addition; Its output is through controller (the typical chip model of control chip control step or driven by servomotor; Being listed as Xiao slightly gets final product down) constant temperature hatches the temperature controller of device; With adopt existing equipments, test card 7, useless card gatherer 9 by stepping or driven by servomotor track drive shaft 3, reference column 4, constant temperature oven 5, ccd image collector 6, the ccd image collector comprises analysis image acquisition system software (available present technology), and by software image is analyzed to judge testing result.The analysis image acquisition system can with controller integral of the present invention; The speed that controller control step or driven by servomotor guide rail drive is accomplished the qualitative or quantitative test parameter etc. of hatching translational speed, IMAQ number of times and the chromogenic reaction of humidity control, reaction time, transmission platform like constant temperature.
Claims (6)
1. collaurum detection method; It is characterized in that being provided with the automatic transmission platform of collaurum test sample, the test card that will add reagent is placed on the automatic transmission platform, and the every interval of transmission platform certain hour moves a position; Test card is hatched the district through constant temperature earlier, carries out IMAQ again; Image acquisition device is adjusted to best image capture position to IMAQ by the displacement regulating system.
2. collaurum detection method according to claim 1; Elder generation is through preparatory acquisition zone before it is characterized in that IMAQ; Acquisition zone is confirmed the chromogenic line exact position of test card in advance; Subsequent picture collector C CD (perhaps CMOS) displacement system is adjusted to best image capture position with image acquisition device CCD (perhaps CMOS) automatically, carries out the analysis image collection.
3. collaurum detection method according to claim 1 is characterized in that image acquisition device by the method for displacement regulating system is: image acquisition device is regulated through a location guide rail: hang and be displaced in the cross recess a certain position and make image acquisition device obtain the accurate position of chromogenic line.
4. collaurum detection method according to claim 1 is characterized in that detect accomplishing the back transmission platform and continues to move, and gathers the test card of image the most at last and delivers in the useless card collection box.
5. full-automatic collaurum detector is characterized in that comprising controller, test card transmission platform, the constant temperature incubator, and magazine, the ccd image collector, the image acquisition device positioning guide rail can also be provided with useless card in addition and collect box; The test card transmission platform adopts travelling belt or transmits disk, and travelling belt or transmission disk increase employing servomotor or step motor drive; Test card places travelling belt successively or transmits the delivering position of disk; Travelling belt or transmit disk a zone above be provided with image acquisition device, image acquisition device is positioned on the location guide rail; Input place of travelling belt or transmission disk is provided with the test card rest area, is the constant temperature incubator after the test card rest area, is provided with useless card collection box at travelling belt or after transmitting disk image acquisition device zone.
6. collaurum pick-up unit according to claim 1 is characterized in that positioning guide rail is provided with image acquisition device suspension and can move a cross recess interior.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201210132692 CN102628874B (en) | 2012-04-28 | 2012-04-28 | Full-automatic colloidal gold detection method |
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| CN 201210132692 CN102628874B (en) | 2012-04-28 | 2012-04-28 | Full-automatic colloidal gold detection method |
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| CN102628874A true CN102628874A (en) | 2012-08-08 |
| CN102628874B CN102628874B (en) | 2013-07-03 |
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| CN 201210132692 Expired - Fee Related CN102628874B (en) | 2012-04-28 | 2012-04-28 | Full-automatic colloidal gold detection method |
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103760338A (en) * | 2014-01-26 | 2014-04-30 | 杭州海士利生物科技有限公司 | Quantitative detection method by using reverse immune chromatography and kit and external equipment |
| WO2015007153A3 (en) * | 2013-07-15 | 2015-03-19 | Abon Biopharm (Hangzhou) Co., Ltd. | An immunoassay reading device and its calibration method |
| CN104714004A (en) * | 2015-03-11 | 2015-06-17 | 厦门为正生物科技有限公司 | Comprehensive information acquisition method for biological detection card |
| CN104836942A (en) * | 2014-11-03 | 2015-08-12 | 中国计量学院 | Colloidal gold portable CCD readout device |
| CN105044336A (en) * | 2015-07-25 | 2015-11-11 | 中山瑞福医疗器械科技有限公司 | Multi-channel detecting instrument |
| CN105203723A (en) * | 2015-07-15 | 2015-12-30 | 宁波高新区绿邦科技发展有限公司 | Device and method for quickly and quantitatively detecting clenbuterol hydrochloride |
| CN108132346A (en) * | 2017-12-30 | 2018-06-08 | 天津博硕东创科技发展有限公司 | A kind of Portable fluorescence immunity analysis instrument |
| CN109490299A (en) * | 2018-09-04 | 2019-03-19 | 广东朗源生物科技有限公司 | A kind of portable mycotoxin colloidal gold detector and application method |
| CN109613286A (en) * | 2018-12-27 | 2019-04-12 | 宁波大学 | A kind of qualitative/quantitative bio-chip test device |
| CN109696439A (en) * | 2019-02-26 | 2019-04-30 | 长沙协大生物科技有限公司 | A kind of urine dry chemical scrip image collecting device |
| CN110220895A (en) * | 2019-06-28 | 2019-09-10 | 中国计量大学 | A kind of image collecting device of high throughput portable colloidal gold CCD readout instrument |
| CN110794151A (en) * | 2019-10-30 | 2020-02-14 | 中山生物工程有限公司 | Method, device, terminal, medium and equipment for detecting quality of blood type card |
| CN112485182A (en) * | 2020-11-16 | 2021-03-12 | 安徽省富鑫雅光电科技有限公司 | LED lamp panel detection device |
| CN115436650A (en) * | 2022-11-08 | 2022-12-06 | 山东康华生物医疗科技股份有限公司 | Full-automatic colloidal gold analyzer |
| CN117420292A (en) * | 2023-11-07 | 2024-01-19 | 青岛华晶生物技术有限公司 | Incubator |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050168747A1 (en) * | 2004-01-29 | 2005-08-04 | Fox Shaw G. | Information-encoded tests and method |
| US20070134812A1 (en) * | 2003-11-07 | 2007-06-14 | Guzman Norberto A | Controlled electrophoresis method |
| CN201051102Y (en) * | 2007-05-29 | 2008-04-23 | 陈晓汀 | Quick tester for gold colloid |
| CN101551340A (en) * | 2009-05-07 | 2009-10-07 | 上海交通大学 | Gold spot quantitative determination optical device |
| CN102253195A (en) * | 2011-06-28 | 2011-11-23 | 上海贝西生物科技有限公司 | Full-automatic immunochromatographic analyzer |
-
2012
- 2012-04-28 CN CN 201210132692 patent/CN102628874B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070134812A1 (en) * | 2003-11-07 | 2007-06-14 | Guzman Norberto A | Controlled electrophoresis method |
| US20050168747A1 (en) * | 2004-01-29 | 2005-08-04 | Fox Shaw G. | Information-encoded tests and method |
| CN201051102Y (en) * | 2007-05-29 | 2008-04-23 | 陈晓汀 | Quick tester for gold colloid |
| CN101551340A (en) * | 2009-05-07 | 2009-10-07 | 上海交通大学 | Gold spot quantitative determination optical device |
| CN102253195A (en) * | 2011-06-28 | 2011-11-23 | 上海贝西生物科技有限公司 | Full-automatic immunochromatographic analyzer |
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015007153A3 (en) * | 2013-07-15 | 2015-03-19 | Abon Biopharm (Hangzhou) Co., Ltd. | An immunoassay reading device and its calibration method |
| CN103760338A (en) * | 2014-01-26 | 2014-04-30 | 杭州海士利生物科技有限公司 | Quantitative detection method by using reverse immune chromatography and kit and external equipment |
| CN103760338B (en) * | 2014-01-26 | 2016-02-03 | 杭州海士利生物科技有限公司 | Quantitative detection method using reverse immunochromatography reaction, kit and external equipment |
| CN104836942B (en) * | 2014-11-03 | 2017-12-19 | 中国计量学院 | The portable CCD readout instruments of collaurum |
| CN104836942A (en) * | 2014-11-03 | 2015-08-12 | 中国计量学院 | Colloidal gold portable CCD readout device |
| CN104714004A (en) * | 2015-03-11 | 2015-06-17 | 厦门为正生物科技有限公司 | Comprehensive information acquisition method for biological detection card |
| CN104714004B (en) * | 2015-03-11 | 2016-07-06 | 厦门为正生物科技股份有限公司 | Biological detection card integrated information acquisition method |
| CN105203723A (en) * | 2015-07-15 | 2015-12-30 | 宁波高新区绿邦科技发展有限公司 | Device and method for quickly and quantitatively detecting clenbuterol hydrochloride |
| CN105044336A (en) * | 2015-07-25 | 2015-11-11 | 中山瑞福医疗器械科技有限公司 | Multi-channel detecting instrument |
| CN108132346A (en) * | 2017-12-30 | 2018-06-08 | 天津博硕东创科技发展有限公司 | A kind of Portable fluorescence immunity analysis instrument |
| CN109490299A (en) * | 2018-09-04 | 2019-03-19 | 广东朗源生物科技有限公司 | A kind of portable mycotoxin colloidal gold detector and application method |
| CN109613286A (en) * | 2018-12-27 | 2019-04-12 | 宁波大学 | A kind of qualitative/quantitative bio-chip test device |
| CN109696439A (en) * | 2019-02-26 | 2019-04-30 | 长沙协大生物科技有限公司 | A kind of urine dry chemical scrip image collecting device |
| CN110220895A (en) * | 2019-06-28 | 2019-09-10 | 中国计量大学 | A kind of image collecting device of high throughput portable colloidal gold CCD readout instrument |
| CN110794151A (en) * | 2019-10-30 | 2020-02-14 | 中山生物工程有限公司 | Method, device, terminal, medium and equipment for detecting quality of blood type card |
| CN112485182A (en) * | 2020-11-16 | 2021-03-12 | 安徽省富鑫雅光电科技有限公司 | LED lamp panel detection device |
| CN115436650A (en) * | 2022-11-08 | 2022-12-06 | 山东康华生物医疗科技股份有限公司 | Full-automatic colloidal gold analyzer |
| CN117420292A (en) * | 2023-11-07 | 2024-01-19 | 青岛华晶生物技术有限公司 | Incubator |
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