CN103760338A - Quantitative detection method by using reverse immune chromatography and kit and external equipment - Google Patents
Quantitative detection method by using reverse immune chromatography and kit and external equipment Download PDFInfo
- Publication number
- CN103760338A CN103760338A CN201410036880.8A CN201410036880A CN103760338A CN 103760338 A CN103760338 A CN 103760338A CN 201410036880 A CN201410036880 A CN 201410036880A CN 103760338 A CN103760338 A CN 103760338A
- Authority
- CN
- China
- Prior art keywords
- kit
- sample
- external unit
- chromatography
- surveyed area
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention discloses a detection method by using reverse immune chromatography and kit and external equipment. According to the method, the reaction amount and reaction time of a sample to be detected are accurately controlled by using the external equipment and kit, so that the reverse immune chromatography is realized. A chromatographic membrane is arranged in the kit, and a contrast area and a detection area are loaded in the middle of the chromatographic membrane; when the external equipment detects that the sample chromatography reaches a specified position, the sample chromatography is finished, the external equipment is automatically controlled to sequentially inject washing liquor and a developing solution or a luminous solution into the kit, and the external equipment controls a liquid suction pump to act on the kit, so that the injected liquid is subjected to reverse chromatography, is sequentially reacted with substances in the contrast area and the detection area and finally generates color change or generates visible light; finally, the external equipment can obtain a quantitative detection result by identifying optical signals in the contrast area and the detection area and comparing the optical signals in the contrast area and the detection area and a built-in standard curve.
Description
Technical field
The present invention relates to a kind of quantitative detecting method and kit and external unit of applying reverse immunochromatography reaction, particularly a kind of quantitative detecting method and kit and external unit and kit and external unit used thereof of applying reverse immunochromatography reaction.
Background technology
At present, a lot of for the detection method of biological specimen, wherein IDEXX company has been used a kind of special kit, and it is stored in washing lotion and nitrite ion in kit, and the top of washing lotion and nitrite ion arranges chromatographic film.Its specific works principle (take milk detection as example) is: heat after 5 minutes (45 ℃) milk sample being put into Reagent Tube, splash in kit, chromatographic film utilizes capillarity that milk sample chromatography is moved to check point and the control point in chromatographic film, material in milk sample and check point and control point is reacted, then observe milk sample chromatography after certain position (detection window place), one side of kit is pressed, because this side of kit is provided with two kapillaries that connect respectively washing lotion pond and chromatographic film and colour developing liquid pool and chromatographic film, after so kit one side is pressed, the washing lotion pond of kapillary puncture through seal and colour developing liquid pool, and washing lotion and nitrite ion are drawn to chromatographic film place, then carry out chromatography and move to check point and control point, check point and control point are carried out to wash-out and colour developing, the color of finally observing check point and control point can draw testing result.The structure of its kit as shown in Figure 1.
But kit is owing to having stored washing lotion and nitrite ion in this, and inner structure is comparatively complicated, and therefore volume is comparatively huge, carries inconvenience.In addition, because general washing lotion and nitrite ion need to keep lower temperature, kit needs all the time stored refrigerated in long-distance transport process, and its transportation cost is higher.And, because the needs realization of the washing lotion in each kit and nitrite ion is encapsulated in kit, its consumption must unify just can be convenient to large-scale production, in order to adapt to the detection of most samples, the encapsulation amount of washing lotion and nitrite ion generally need be greater than actual use amount, so can cause very large waste.In addition, because this scheme is substantially all to utilize the capillary action of chromatographic film self that liquid is circulated, so kit is to the having relatively high expectations of chromatographic film, need its inside to there is abundant kapillary, and need to keep away from moisture in preserving.Therefore IDEXX company chromatographic film used is also generally special, and its production cost and retain costs are higher.It will be further appreciated that, this scheme can only qualitative detection sample, can not export quantitative result, so can only judge negative positively, can not export definite concentration value.
Summary of the invention
The object of the invention is to, a kind of quantitative detecting method and kit and external unit of applying reverse immunochromatography reaction is provided.It can solve that the kit existing in prior art cannot be exported quantitative result, carries inconvenience, transportation cost and retain costs is high and the problem such as washing lotion and nitrite ion waste.
Technical scheme of the present invention: apply the quantitative detecting method of reverse immunochromatography reaction, be characterized in: use external unit and reacting dose and the reaction time of kit to sample to be tested accurately to control, thereby realize reverse immunochromatography reaction, in this agent box, be provided with chromatographic film, chromatographic film middle part is loaded with control zone and surveyed area, when detecting sample chromatography, external unit arrives assigned address, sample chromatography completes, automatically control external unit washing lotion and nitrite ion or luminescent solution are successively injected to kit, peripheral equipment control suction pumps acts on kit, make the liquid injecting carry out reverse chromatography, and successively react (immune response and/or chemical reaction) with the material in control zone and surveyed area, and finally produce change color or produce visible ray, last external unit is by the light signal of identification control zone and surveyed area, and after being contrasted, the light signal of control zone and surveyed area and built-in typical curve can draw quantitative testing result.
Built-in typical curve is pre-stored in external unit, and it is the weak corresponding curve (can sum up matching according to many experiments forms) with test substance concentration in sample of a bars light intensity.Therefore recording after light signal strength and can correspondence obtain concentration information.
In the quantitative detecting method of the reverse immunochromatography reaction of above-mentioned application, the concrete steps of pattern detection comprise:
1. in the well of the wherein side of kit, carry out quantitative application of sample, sample chromatography under the capillary action of chromatographic film moves to control zone and surveyed area, and reacts with the material in control zone and surveyed area;
2. when external unit detects that sample to be tested chromatography is to giving directions position, external unit first injects washing lotion again in the liquid injection hole of the opposite side of kit, and on well, uses suction pumps to aspirate, and makes the reverse chromatography of washing lotion move to well;
3. then in liquid injection hole, add nitrite ion or luminescent solution, suction pumps works on simultaneously, makes nitrite ion or luminescent solution and washing lotion carry out chromatography in the same way, then on control zone and surveyed area, chromogenic reaction occurs;
4. the light signal (comprise color signal) of last external unit to surveyed area and control zone identified automatically, after final external unit compares to the strong and weak and built-in typical curve of signal, draw quantitative testing result, and testing result is shown to user.
In the quantitative detecting method of the reverse immunochromatography reaction of aforesaid application, described surveyed area and control zone are for being loaded with point or the line of reactant (latex/magnetic bead/antibody).
For the kit of preceding method, be characterized in: comprise the chromatographic film of bar shaped, chromatographic film is coated with box body, the middle part of box body is provided with detection window, and the chromatographic film of detection window position is provided with control zone and surveyed area; On the box body of described detection window both sides, offer respectively well for adding sample and for adding the liquid injection hole of washing lotion and nitrite ion or luminescent solution, liquid injection hole is also for detection of the chromatography position of sample; In described control zone and surveyed area, be loaded with respectively the reactant reacting with sample.
For the external unit of preceding method, be characterized in: at least comprise a floor component, floor component is provided with bracket component, and bracket component below is provided with for holding kit and slidably passing in and out the board supporting plate of external unit, is fixed with imbibition parts and liquid feeding parts on bracket component; Bracket component is also fixed with barcode detection parts, image-detection component; Also comprise display unit and control the control mainboard of each parts.
In aforesaid external unit, also comprise IC-card read-write parts, heater block and fluorescent scanning detection part.
Compared with prior art, the present invention is used in conjunction with to realize testing goal by an external unit and a more easy kit.By the monitoring to liquid injection hole, the position and the time that by external unit, can automatic analysis go out sample chromatography, automatically control the opportunity that adds washing lotion and nitrite ion, when finishing, surveyed area reaction adds washing lotion, cessation reaction.Compared with prior art item, because machine is controlled automatically, prevent scrapping of overreaction generation, improved the precision that reagent detects, prevent the unnecessary error that manual operation causes.With respect to prior art, the interpolation of nitrite ion and washing lotion and the hand over of preservation are to external unit.So kit of the present invention can do very thinly, volume is very little, is easy to carry, and has there is no the preservation condition constraint of washing lotion and nitrite ion, and transportation, retain costs have also reduced a lot.In addition, because external unit can be preserved a large amount of nitrite ions and washing lotion, its addition also can accurately be controlled by external unit, and an external unit can repeat different kit effects, compared with prior art, can save a large amount of washing lotions and nitrite ion.And the present invention is also provided with imbibition parts in external unit, in the multiple steps that detect, use imbibition parts to act on chromatographic film, make liquid carry out passively chromatography, thereby reduced the requirement to the performance of chromatographic film (being equivalent to chromatographic film of the prior art) own, strengthen washability simultaneously, reduced nonspecific reaction.The present invention is when carrying out pattern detection, only need kit to insert external unit, again sample to be tested is added to external unit, other step all can be controlled automatically by external unit, finally can in the display system of external unit, obtain intuitively quantitative testing result, the error of having avoided people's subjective judgement to produce.
Fig. 1 is the structural drawing of available reagent box;
Fig. 2 is the structural representation of kit of the present invention;
Fig. 3 is the structured flowchart of external unit of the present invention;
Fig. 4 is the side view of Fig. 3.
Being labeled as in accompanying drawing: 1-well, 2-detection window, 3-chromatographic film, 4-liquid injection hole, 5-box body, 6-control zone, 7-surveyed area, 11-floor component, 12-bracket component, 13-imbibition parts, 14-liquid feeding parts, 15-image-detection component, 16-board supporting plate, 17-IC card read-write parts, 18-fluorescent scanning detection part, 110-display unit, 111-heater block, 112-controls mainboard.
Below in conjunction with drawings and Examples, the present invention is further illustrated, but not as the foundation to the present invention's restriction.
Embodiment.Apply the detection method of reverse immunochromatography reaction: use external unit and reacting dose and the reaction time of kit to sample to be tested accurately to control, thereby realize reverse immunochromatography reaction, in this agent box, be provided with chromatographic film, chromatographic film middle part is loaded with control zone and surveyed area, when detecting sample chromatography, external unit arrives assigned address, sample chromatography completes, automatically control external unit washing lotion and nitrite ion or luminescent solution are successively injected to kit, peripheral equipment control suction pumps acts on kit, make the liquid injecting carry out reverse chromatography, and successively react (immune response and/or chemical reaction) with the material in control zone and surveyed area, and finally produce change color or produce visible ray, last external unit is by the light signal of identification control zone and surveyed area, after the strong and weak and built-in typical curve of contrast signal, can draw quantitative testing result.
The concrete steps of pattern detection comprise:
1. in the well of the wherein side of kit, carry out quantitative application of sample, sample chromatography under the capillary action of chromatographic film moves to control zone and surveyed area, and reacts with the material in control zone and surveyed area;
2. when external unit detects that sample to be tested chromatography is to giving directions position, external unit first injects washing lotion again in the liquid injection hole of the opposite side of kit, and on well, uses suction pumps to aspirate, and makes the reverse chromatography of washing lotion move to well;
3. then in liquid injection hole, add nitrite ion or luminescent solution, suction pumps works on simultaneously, makes nitrite ion or luminescent solution and washing lotion carry out chromatography in the same way, then on control zone and surveyed area, chromogenic reaction occurs;
4. the light signal (comprise color signal) of last external unit to surveyed area and control zone identified automatically, after final external unit compares to the strong and weak and built-in typical curve of signal, and testing result is shown to user.
Described surveyed area and control zone are for being loaded with point or the line of reactant (latex/magnetic bead/antibody).
For the kit of preceding method, as shown in Figure 2: comprise the chromatographic film 3 of bar shaped, chromatographic film 3 is coated with box body 5, and the middle part of box body 5 is provided with detection window 2, the chromatographic film 3 of detection window 2 positions is provided with control zone 6 and surveyed area 7; On the box body 5 of described detection window 2 both sides, offer respectively well 1 for adding sample and for adding the liquid injection hole 4 of washing lotion and nitrite ion or luminescent solution, liquid injection hole 4 is also for detection of the chromatography position of sample; The interior reactant reacting with sample that is loaded with respectively of described control zone 6 and surveyed area 7.
For the external unit of preceding method, as shown in Figure 3 and Figure 4: at least comprise a floor component 11, floor component 11 is provided with bracket component 12, bracket component 12 belows are provided with for holding kit and slidably passing in and out the board supporting plate 16 of external unit, are fixed with imbibition parts 13 and liquid feeding parts 14 on bracket component 12; Bracket component 12 is also fixed with barcode detection parts 19, image-detection component 15; Also comprise display unit 110 and control the control mainboard 112 of each parts.Also comprise IC-card read-write parts 17, heater block 111 and fluorescent scanning detection part 18.The position relationship at external unit of each parts has no special requirements, and only needing to realize following principle of work can arrange arbitrarily.
The principle of work of external unit is as follows, take milk sample as example, milk sample is added to sample hose, slightly shakes up, then sample hose is put into the heater block 111 of external unit, heating 5min; Sample hose is taken out from heater block 111, milk is poured into the well 1 of kit, sample starts chromatography and moves, kit is put into board supporting plate 16 simultaneously, board supporting plate 16 enters external unit, bar code in barcode detection parts 19 detection kit, then image-detection component 15 detects liquid injection hole automatically, detects sample and whether arrives liquid injection hole; When detecting that sample arrives liquid injection hole, imbibition parts 13 move to well, and start imbibition, and liquid feeding parts 14 are started working simultaneously, add washing lotion; After washing lotion chromatography completes, imbibition parts work on, and add nitrite ion and start chromatography.After nitrite ion chromatography completes, kit moves to detecting position, by image-detection component 15, starts to detect (while adding luminescent solution, can use fluorescent scanning detection part 18 to detect), by controlling mainboard 112, coordinate software analysis to provide testing result, and result is presented on display unit 110.
Now, take milk pattern detection as example, it is as follows that it specifically detects principle.
1, explanation.
1) in reaction tube, comprise PBP-HRP(PBP-horseradish peroxidase and connect albumen, be called for short PBP-HRP) be connected albumen with the anti-rat immune globulin-horseradish peroxidase of R-anti-M IgG-HRP(rabbit, be called for short RAM-HRP) freeze-dried powder;
2) check point: by solid-phase coating technology, little ampicillin molecule (Amp) is fixed to micro-nano latex particle and (is called for short: Latex) be above called for short Latex-Amp, get certain density coated latex point sample on chromatographic film.
3) control point comprises 2 schemes.
Option A: by solid-phase coating technology, mouse IgG (immunoglobulin (Ig)) is fixed on micro-nano latex particle and (is write a Chinese character in simplified form: Latex-M IgG), get certain density coated latex point sample on chromatographic film.Now, the control antibodies being included in reaction tube is the horseradish peroxidase bond (writing a Chinese character in simplified form R-anti-M IgG-HRP) of the anti-mouse IgG of rabbit.
Option b: by solid-phase coating technology, anti-mouse PBP protein antibodies is fixed on micro-nano latex particle and (is write a Chinese character in simplified form: Latex-Anti PBP), go certain density coated latex point sample on chromatographic film.Now, be included in reaction tube without control antibodies, only have PBP-HRP.
2, course of reaction
1) get 400ul milk sample to be measured, join in reaction tube, mix, the sample that joins external unit adds mouth, 45 ℃ of heating 5min.
The reaction wherein existing:
A) negative milk: any composition in multiple PBP-HRP and the milk melting does not react, and is still present in milk with the PBP-HRP albumen that dissociates.
B) positive milk: the microbiotic reaction (take benzyl penicillin (Penicillin G) as example) in multiple PBP-HRP and the milk melting of part, form Penicillin G-PBP-HRP compound, be present in milk, part PBP-HRP still exists in the mode of dissociating, if high containing Penicillin G concentration in milk to be measured, free PBP-HRP concentration is low.
C), in option A, R-anti-M IgG-HRP does not participate in reaction.
2) after reaction finishes, 400ul milk is joined in the well of chromatographic film, milk to be measured is in the mode of chromatography, and within the regular hour, Rapid Flow is crossed the position of check point and control point, and arrives the position in washing lotion hole.
In option A, the reaction that wherein check point and control point exist:
A) negative milk: be present in the free PBP-HRP albumen in Fresh Milk, and Latex-Amp reaction in check point, form Latex-Amp-PBP-HRP compound, be fixed on check point.
B) positive milk: Penicillin G-PBP-HRP, owing to reacting, when flowing through check point, does not react; And the PBP-HRP that part still exists in the mode of dissociating, with Latex-Amp reaction, forms Latex-Amp-PBP-HRP compound and is fixed on check point.Latex-Amp-PBP-HRP compound amount number, depend on the amount of Penicillin G in sample number, in sample, Penicillin G amount is many, Latex-Amp-PBP-HRP compound amount is few, otherwise otherwise.
C) the milk control point of flowing through, R-anti-M IgG-HRP and Latex-M IgG reaction, form Latex-M IgG-R-anti-M IgG-HRP, is fixed on control point.
In option b, the reaction of check point and control point:
A) negative milk: be present in the free PBP-HRP albumen in Fresh Milk, respectively with check point in Latex-Amp, Latex-Anti PBP in control point reaction, forms Latex-Amp-PBP-HRP compound, is fixed on check point; Form Latex-Anti PBP-PBP-HRP compound and be fixed on control point.
B) positive milk: Penicillin G-PBP-HRP, owing to reacting, when flowing through check point, does not react with latex-Amp; And the PBP-HRP that part still exists in the mode of dissociating, with Latex-Amp reaction, forms Latex-Amp-PBP-HRP compound and is fixed on check point.Latex-Amp-PBP-HRP compound amount number, depend on the amount of Penicillin G in sample number, in sample, Penicillin G amount is many, Latex-Amp-PBP-HRP compound amount is few, otherwise otherwise.When the positive milk control point of flowing through, Latex-Anti PBP and Penicillin G-PBP-HRP and free PBP-HRP react, and form Latex-Anti PBP-Penicillin G-PBP-HRP compound and Latex-Anti PBP-PBP-HRP compound and are fixed on control point.In sample, Penicillin G concentration is higher, form Penicillin G-PBP-HRP compound amount more, free PBP-HRP amount is fewer, and in check point, Latex-Amp-PBP-HRP compound amount is few, but control point Latex-Anti PBP-Penicillin G-PBP-HRP is more.
3) washing: when reaction finishes, suction pumps arrives well, and a certain amount of washing lotion joins in washing lotion hole, suction pumps work, washing reaction film
4) colour developing: washing finishes, and adds a certain amount of nitrite ion in washing lotion hole, suction pumps work, colour developing 3min
5) detect judgement.Image identification system reads check point and control point gray-scale value automatically, and instrument internal arranges typical curve, according to the gray-scale value of check point and control point, and with reference to built-in typical curve, draws quantitative concentration value.
Milk in embodiment 1 detects, method and apparatus of the present invention is also applicable to other sample and test item of utilizing directed fast immune chromatographic detection method to detect, and the sample that in background technology, the kit of IDEXX company can detect and test item all can be used method and apparatus of the present invention to detect.The sample that the present invention can detect mainly comprises: blood, serum, blood plasma, urine, chest ascites, hydrocrania, cells and supernatant, sputum, throat swab eluent, Fresh Milk, finished product milk, milk powder, animal vegetable tissue homogenate, animal blood, animal blood serum, animals urine and other body fluid of animal etc.The present invention can be for detection of people and animal blood sample, body fluid sample; in food liquid, animals and plants extract, detect pathogen, albumen, other molecules or chemical molecular etc.; step in detecting step and embodiment 1 is basic identical, only needs material and corresponding replacement washing lotion and nitrite ion in check point in replacement equipment, control point.
Claims (6)
1. the quantitative detecting method of the reverse immunochromatography reaction of application, is characterized in that: use external unit and reacting dose and the reaction time of kit to sample to be tested accurately to control, thereby realize reverse immunochromatography reaction, in this agent box, be provided with chromatographic film, chromatographic film middle part is loaded with control zone and surveyed area, when detecting sample chromatography, external unit arrives assigned address, sample chromatography completes, automatically control external unit washing lotion and nitrite ion or luminescent solution are successively injected to kit, peripheral equipment control suction pumps acts on kit, make the liquid injecting carry out reverse chromatography, and successively react with the material in control zone and surveyed area, and finally produce change color or produce visible ray, last external unit is by the light signal of identification control zone and surveyed area, and after being contrasted, the light signal of control zone and surveyed area and built-in typical curve can draw quantitative testing result.
2. the quantitative detecting method of the reverse immunochromatography reaction of application according to claim 1, is characterized in that, the concrete steps of pattern detection comprise:
1. in the well of the wherein side of kit, carry out quantitative application of sample, sample chromatography under the capillary action of chromatographic film moves to control zone and surveyed area, and reacts with the material in control zone and surveyed area;
2. when external unit detects that sample to be tested chromatography is to giving directions position, external unit first injects washing lotion again in the liquid injection hole of the opposite side of kit, and on well, uses suction pumps to aspirate, and makes the reverse chromatography of washing lotion move to well;
3. then in liquid injection hole, add nitrite ion or luminescent solution, suction pumps works on simultaneously, makes nitrite ion or luminescent solution and washing lotion carry out chromatography in the same way, then on control zone and surveyed area, chromogenic reaction occurs;
4. last external unit is identified automatically to the light signal of surveyed area and control zone, and final external unit is shown to user by quantitative testing result after the strong and weak and built-in typical curve of signal is compared.
3. the quantitative detecting method of the reverse immunochromatography reaction of application according to claim 1 and 2, is characterized in that: described surveyed area and control zone are point or the line that is loaded with reactant.
4. for the kit of method described in the arbitrary claim of claims 1 to 3, it is characterized in that: the chromatographic film (3) that comprises bar shaped, chromatographic film (3) is coated with box body (5), the middle part of box body (5) is provided with detection window (2), and the chromatographic film (3) of detection window (2) position is provided with control zone (6) and surveyed area (7); On the box body (5) of described detection window (2) both sides, offer respectively well (1) for adding sample and for adding the liquid injection hole (4) of washing lotion and nitrite ion or luminescent solution, liquid injection hole (4) is also for detection of the chromatography position of sample; In described control zone (6) and surveyed area (7), be loaded with respectively the reactant reacting with sample.
5. for the external unit of method described in the arbitrary claim of claims 1 to 3, it is characterized in that: at least comprise a floor component (11), floor component (11) is provided with bracket component (12), bracket component (12) below is provided with for holding kit and slidably passing in and out the board supporting plate (16) of external unit, is fixed with imbibition parts (13) and liquid feeding parts (14) on bracket component (12); Bracket component (12) is also fixed with barcode detection parts (19), image-detection component (15); Also comprise display unit (110) and control the control mainboard (112) of each parts.
6. external unit according to claim 5, is characterized in that: also comprise IC-card read-write parts (17), heater block (111) and fluorescent scanning detection part (18).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410036880.8A CN103760338B (en) | 2014-01-26 | 2014-01-26 | Apply quantitative detecting method and kit and the external unit of the reaction of reverse immunochromatography |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410036880.8A CN103760338B (en) | 2014-01-26 | 2014-01-26 | Apply quantitative detecting method and kit and the external unit of the reaction of reverse immunochromatography |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103760338A true CN103760338A (en) | 2014-04-30 |
| CN103760338B CN103760338B (en) | 2016-02-03 |
Family
ID=50527611
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410036880.8A Active CN103760338B (en) | 2014-01-26 | 2014-01-26 | Apply quantitative detecting method and kit and the external unit of the reaction of reverse immunochromatography |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103760338B (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1786712A (en) * | 2004-12-07 | 2006-06-14 | 盛进生物工程(深圳)有限公司 | Method and system for quantitatively fast level testing flow |
| CN1837823A (en) * | 2005-03-21 | 2006-09-27 | 邓雪梅 | Animal epidemic disease detection kit and detection method thereof |
| CN201600367U (en) * | 2009-12-01 | 2010-10-06 | 刘江 | General immunity chromatography card interpretation recorder |
| CN102253195A (en) * | 2011-06-28 | 2011-11-23 | 上海贝西生物科技有限公司 | Full-automatic immunochromatographic analyzer |
| CN102628874A (en) * | 2012-04-28 | 2012-08-08 | 无锡瑞美电子科技有限公司 | Full-automatic colloidal gold detection method and full-automatic colloidal gold detection instrument |
| WO2012141368A2 (en) * | 2011-04-13 | 2012-10-18 | Philosys Co., Ltd. | Test strip analyzing apparatus having wheel ejector |
| EP2677321A1 (en) * | 2012-06-20 | 2013-12-25 | Innogenetics N.V. | Integrated device for nucleic acid hybridizations and immuno-assays |
| CN203672893U (en) * | 2014-01-26 | 2014-06-25 | 杭州海士利生物科技有限公司 | External device for quantitative detection of sample |
| CN203672884U (en) * | 2014-01-26 | 2014-06-25 | 杭州海士利生物科技有限公司 | Kit for quantitative detection of sample |
-
2014
- 2014-01-26 CN CN201410036880.8A patent/CN103760338B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1786712A (en) * | 2004-12-07 | 2006-06-14 | 盛进生物工程(深圳)有限公司 | Method and system for quantitatively fast level testing flow |
| CN1837823A (en) * | 2005-03-21 | 2006-09-27 | 邓雪梅 | Animal epidemic disease detection kit and detection method thereof |
| CN201600367U (en) * | 2009-12-01 | 2010-10-06 | 刘江 | General immunity chromatography card interpretation recorder |
| WO2012141368A2 (en) * | 2011-04-13 | 2012-10-18 | Philosys Co., Ltd. | Test strip analyzing apparatus having wheel ejector |
| CN102253195A (en) * | 2011-06-28 | 2011-11-23 | 上海贝西生物科技有限公司 | Full-automatic immunochromatographic analyzer |
| CN102628874A (en) * | 2012-04-28 | 2012-08-08 | 无锡瑞美电子科技有限公司 | Full-automatic colloidal gold detection method and full-automatic colloidal gold detection instrument |
| EP2677321A1 (en) * | 2012-06-20 | 2013-12-25 | Innogenetics N.V. | Integrated device for nucleic acid hybridizations and immuno-assays |
| CN203672893U (en) * | 2014-01-26 | 2014-06-25 | 杭州海士利生物科技有限公司 | External device for quantitative detection of sample |
| CN203672884U (en) * | 2014-01-26 | 2014-06-25 | 杭州海士利生物科技有限公司 | Kit for quantitative detection of sample |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103760338B (en) | 2016-02-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104428675B (en) | For detection property immediately (POCT) immunochromatographiassays assays lyophilizing conjugate structure body, comprise its immunity detection reagent, and use the method that this test kit is analyzed | |
| CN101120253B (en) | Immunochromatographic test instrument and semiquantitative method using the same | |
| CN109647553A (en) | Multi objective disease joint-detection micro fluidic device | |
| Molina-Díaz et al. | Solid-phase spectroscopy from the point of view of green analytical chemistry | |
| JPH03135768A (en) | Analyzing method and automatic analyzer | |
| CN106153927A (en) | A kind of fast quantification detects time-resolved fluoroimmunoassay chromatography reagent and the preparation method of cTnI, CKMB, Myo simultaneously | |
| CN205650212U (en) | A double -deck micro -fluidic chip of magnetic particle chemiluminescence for whole blood sample test | |
| CN104169710A (en) | Method and apparatus for time-resolved fluorescence immunoassay testing | |
| CN207717778U (en) | TNF-α time-resolved fluoroimmunoassay chromatographs immue quantitative detection reagent box | |
| JP2013076713A (en) | Manufacturing method of aggregation reagent, aggregation reagent or product material produced thereby, and analysis object measuring method with the same, and test kit and analysis device | |
| CN105988008B (en) | Determine device, kit and method | |
| CN106841631A (en) | Cardiac muscle troponin I/N ends Natriuretic Peptide/D dimer is three-in-one to determine kit and preparation method | |
| CN107677833A (en) | A kind of cardiac muscle troponin I magnetic microparticle chemiluminescence measure kit and its application method | |
| US20220184619A1 (en) | Magnetic particle luminescent micro-fluidic chip for multi-marker detection and detection apparatus | |
| CN101038255B (en) | Capillary electrophoresis chemiluminescence detector of acridiniumester, acridine sulfonamide and marker thereof, and method thereof | |
| García‐Campaña et al. | Detection in the liquid phase applying chemiluminescence | |
| CN203672893U (en) | External device for quantitative detection of sample | |
| CN111398490A (en) | Kit for detecting free triiodothyronine and free thyroxine by mass spectrometry | |
| CN106990254A (en) | 25 hydroxycholecalciferols determine kit and preparation method | |
| CN113156105A (en) | A type botulinum toxin rapid quantitative detection card | |
| CN111707818A (en) | Immunochromatography detection card, preparation method and detection method | |
| CN106872716A (en) | Serum amyloid A protein and two-in-one measure kit and the preparation method of Procalcitonin | |
| CN203672884U (en) | Kit for quantitative detection of sample | |
| CN101458252A (en) | Dairy antibiotic rapid detection kit | |
| CN113687087A (en) | Parathyroid hormone rapid detection card and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |