CN203672893U - External device for quantitative detection of sample - Google Patents
External device for quantitative detection of sample Download PDFInfo
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- CN203672893U CN203672893U CN201420049321.6U CN201420049321U CN203672893U CN 203672893 U CN203672893 U CN 203672893U CN 201420049321 U CN201420049321 U CN 201420049321U CN 203672893 U CN203672893 U CN 203672893U
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- hrp
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Abstract
The utility model discloses an external device for quantitative detection of a sample. The external device at least comprises a floor component (11), wherein a bracket component (12) is arranged on the floor component (11), a board tray (16) arranged below the bracket component (12), the board tray (16) accommodates a lit and can slide into or out of the external device, a liquid suction component (13) and a liquid adding component (14) are fixed on the bracket component (12), and a bar code detection component (19) and an image detection component (15) are further fixed on the bracket component (12); the external device further comprises a display component (110) and a control mainboard (112) for controlling each component. The external device can be used for automatically adding liquid into the kit, controlling reaction time and automatically acquiring a quantitative detection result.
Description
Technical field
The utility model relates to a kind of external unit quantitatively detecting for sample, particularly a kind of for applying the external unit of detection method of reverse immunochromatography reaction.
Background technology
At present, a lot of for the detection method of biological specimen, wherein IDEXX company has been used a kind of special kit, and it is stored in washing lotion and nitrite ion in kit, and the top of washing lotion and nitrite ion arranges chromatographic film.Its specific works principle (taking milk detection as example) is: heat after 5 minutes (45 DEG C) milk sample being put into Reagent Tube, splash in kit, chromatographic film utilizes capillarity that milk sample chromatography is moved to check point and the control point in chromatographic film, material in milk sample and check point and control point is reacted, then observe milk sample chromatography after certain position (detection window place), one side of kit is pressed, because this side of kit is provided with two kapillaries that connect respectively washing lotion pond and chromatographic film and colour developing liquid pool and chromatographic film, after so kit one side is pressed, the washing lotion pond of kapillary puncture through seal and colour developing liquid pool, and washing lotion and nitrite ion are drawn to chromatographic film place, then carry out chromatography and move to check point and control point, check point and control point are carried out to wash-out and colour developing, the color of finally observing check point and control point can draw testing result.The structure of its kit as shown in Figure 3.
But kit is owing to having stored washing lotion and nitrite ion in this, and inner structure is comparatively complicated, and therefore volume is comparatively huge, carries inconvenience.In addition, because general washing lotion and nitrite ion need to keep lower temperature, kit needs all the time stored refrigerated in long-distance transport process, and its transportation cost is higher.And, because the needs realization of the washing lotion in each kit and nitrite ion is encapsulated in kit, its consumption must unify just can be convenient to large-scale production, in order to adapt to the detection of most samples, the encapsulation amount of washing lotion and nitrite ion generally need be greater than actual use amount, so can cause very large waste.It will be further appreciated that, because this scheme is substantially all to utilize the capillary action of chromatographic film self that liquid is circulated, so kit is to the having relatively high expectations of chromatographic film, need its inside to there is abundant kapillary, and need to keep away from moisture in preserving.Therefore IDEXX company chromatographic film used is also generally special, and its production cost and retain costs are higher.
Utility model content
The purpose of this utility model is, a kind of external unit quantitatively detecting for sample is provided.The utility model can carry out liquid feeding, reaction time control to kit automatically, and can the quantitative testing result of automatic acquisition.
The technical solution of the utility model: the external unit quantitatively detecting for sample, be characterized in: at least comprise a floor component, floor component is provided with bracket component, bracket component below is provided with for holding kit and slidably passing in and out the board supporting plate of external unit, is fixed with imbibition parts and liquid feeding parts on bracket component; Bracket component is also fixed with barcode detection parts, image-detection component; Also comprise display unit and control the control mainboard of each parts.
The aforesaid external unit quantitatively detecting for sample, also comprises IC-card read-write parts, heater block and fluorescent scanning detection part.
Compared with prior art, the utility model the utility model can carry out liquid feeding, reaction time control to kit automatically, and can the quantitative testing result of automatic acquisition.Compared with prior art item, because machine is controlled automatically, prevent scrapping of overreaction generation, improve the precision that reagent detects, prevent the unnecessary error that manual operation causes.With respect to prior art, the interpolation of nitrite ion and washing lotion and the hand over of preservation are to external unit.So kit of the present invention can do very thinly, volume is very little, is easy to carry, and has there is no the preservation condition constraint of washing lotion and nitrite ion, and transport, retain costs have also reduced a lot.In addition, because external unit can be preserved a large amount of nitrite ions and washing lotion, its addition also can accurately be controlled by external unit, and an external unit can repeat different kit effects, compared with prior art, can save a large amount of washing lotions and nitrite ion.And the present invention is also provided with imbibition parts in external unit, in the multiple steps that detect, use imbibition parts to act on chromatographic film, make liquid carry out passively chromatography, thereby reduce the requirement to the performance of chromatographic film (being equivalent to chromatographic film of the prior art) own, strengthen washability simultaneously, reduced nonspecific reaction.The present invention is in the time carrying out pattern detection, only need kit to insert external unit, again sample to be tested is added to external unit, other step all can be controlled automatically by external unit, finally can in the display system of external unit, obtain intuitively quantitative testing result, the error of having avoided people's subjective judgement to produce.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model;
Fig. 2 is the side view of Fig. 1;
Fig. 3 is the structural drawing of available reagent box;
Fig. 4 is the structural representation of kit of the present invention.
Being labeled as in accompanying drawing: 1-well, 2-detection window, 3-chromatographic film, 4-liquid injection hole, 5-box body, 6-control zone, 7-surveyed area, 11-floor component, 12-bracket component, 13-imbibition parts, 14-liquid feeding parts, 15-image-detection component, 16-board supporting plate, 17-IC card read-write parts, 18-fluorescent scanning detection part, 110-display unit, 111-heater block, 112-controls mainboard.
Embodiment
Below in conjunction with drawings and Examples, the utility model is further described, but not as the foundation to the utility model restriction.
Embodiment.The external unit quantitatively detecting for sample, as depicted in figs. 1 and 2: at least comprise a floor component 11, floor component 11 is provided with bracket component 12, bracket component 12 belows are provided with for holding kit and slidably passing in and out the board supporting plate 16 of external unit, are fixed with imbibition parts 13 and liquid feeding parts 14 on bracket component 12; Bracket component 12 is also fixed with barcode detection parts 19, image-detection component 15; Also comprise display unit 110 and control the control mainboard 112 of each parts.Also comprise IC-card read-write parts 17, heater block 111 and fluorescent scanning detection part 18.The position relationship at external unit of each parts has no special requirements, and only needing to realize following principle of work can arrange arbitrarily.
The utility model can detect the kit of structure as shown in Figure 4, this kit comprises the chromatographic film 3 of bar shaped, chromatographic film 3 is coated with box body 5, and the middle part of box body 5 is provided with detection window 2, and the chromatographic film 3 of detection window 2 positions is provided with control zone 6 and surveyed area 7; On the box body 5 of described detection window 2 both sides, offer respectively well 1 for adding sample and for adding the liquid injection hole 4 of washing lotion and nitrite ion or luminescent solution, liquid injection hole 4 is also for detection of the chromatography position of sample; The interior reactant reacting with sample that is loaded with respectively of described control zone 6 and surveyed area 7.
The principle of work of external unit is as follows, taking milk sample as example, milk sample is added to sample hose, slightly shakes up, then sample hose is put into the heater block 111 of external unit, heating 5min; Sample hose is taken out from heater block 111, milk is poured into the well 1 of kit, sample starts chromatography and moves, kit is put into board supporting plate 16 simultaneously, board supporting plate 16 enters external unit, bar code in barcode detection parts 19 detection kit, then image-detection component 15 detects liquid injection hole automatically, detects sample and whether arrives liquid injection hole; When detecting that sample arrives liquid injection hole, imbibition parts 13 move to well, and start imbibition, and liquid feeding parts 14 are started working simultaneously, add washing lotion; After washing lotion chromatography completes, imbibition parts work on, and add nitrite ion and start chromatography.After nitrite ion chromatography completes, kit moves to detecting position, started to detect by image-detection component 15 and obtain light intensity signal (while adding luminescent solution, can use fluorescent scanning detection part 18 to detect), coordinate software analysis to provide testing result by controlling mainboard 112, and result is presented on display unit 110.Can built-in typical curve in the control mainboard of external unit, typical curve is the weak corresponding curve (can sum up matching according to many experiments forms) with test substance concentration in sample of a bars light intensity.Therefore recording after light signal strength and can correspondence obtain concentration information.Thereby realize the object quantitatively detecting.
Now, taking milk pattern detection as example, it is as follows that it specifically detects principle.
1, explanation.
1) in reaction tube, comprise PBP-HRP(PBP-horseradish peroxidase and connect albumen, be called for short PBP-HRP) be connected albumen with the anti-rat immune globulin-horseradish peroxidase of R-anti-M IgG-HRP(rabbit, be called for short RAM-HRP) freeze-dried powder;
2) check point: by solid-phase coating technology, little ampicillin molecule (Amp) is fixed to micro-nano latex particle and (is called for short: Latex) be above called for short Latex-Amp, get certain density coated latex point sample on chromatographic film.
3) control point comprises 2 schemes.
Option A: by solid-phase coating technology, mouse IgG (immunoglobulin (Ig)) is fixed on micro-nano latex particle and (is write a Chinese character in simplified form: Latex-M IgG), get certain density coated latex point sample on chromatographic film.Now, the control antibodies being included in reaction tube is the horseradish peroxidase bond (writing a Chinese character in simplified form R-anti-M IgG-HRP) of the anti-mouse IgG of rabbit.
Option b: by solid-phase coating technology, anti-mouse PBP protein antibodies is fixed on micro-nano latex particle and (is write a Chinese character in simplified form: Latex-Anti PBP), go certain density coated latex point sample on chromatographic film.Now, be included in reaction tube without control antibodies, only have PBP-HRP.
2, course of reaction
1) get 400ul milk sample to be measured, join in reaction tube, mix, the sample that joins external unit adds mouth, 45 DEG C of heating 5min.
The reaction wherein existing:
A) negative milk: any composition in multiple PBP-HRP and the milk melting does not react, and is still present in milk with the PBP-HRP albumen that dissociates.
B) positive milk: the microbiotic reaction (taking benzyl penicillin (Penicillin G) as example) in multiple PBP-HRP and the milk melting of part, form Penicillin G-PBP-HRP compound, be present in milk, part PBP-HRP still exists in the mode of dissociating, if high containing Penicillin G concentration in milk to be measured, free PBP-HRP concentration is low.
C), in option A, R-anti-M IgG-HRP does not participate in reaction.
2) after reaction finishes, 400ul milk is joined in the well of chromatographic film, milk to be measured is in the mode of chromatography, and within the regular hour, Rapid Flow is crossed the position of check point and control point, and arrives the position in washing lotion hole.
In option A, the reaction that wherein check point and control point exist:
A) negative milk: be present in the free PBP-HRP albumen in Fresh Milk, and Latex-Amp reaction in check point, form Latex-Amp-PBP-HRP compound, be fixed on check point.
B) positive milk: Penicillin G-PBP-HRP, owing to reacting, in the time flowing through check point, does not react; And the PBP-HRP that part still exists in the mode of dissociating, with Latex-Amp reaction, forms Latex-Amp-PBP-HRP compound and is fixed on check point.Latex-Amp-PBP-HRP compound amount number, depend on the amount of Penicillin G in sample number, in sample, Penicillin G amount is many, Latex-Amp-PBP-HRP compound amount is few, otherwise otherwise.
C) the milk control point of flowing through, R-anti-M IgG-HRP and Latex-M IgG reaction, form Latex-M IgG-R-anti-M IgG-HRP, is fixed on control point.
In option b, the reaction of check point and control point:
A) negative milk: be present in the free PBP-HRP albumen in Fresh Milk, respectively with check point in Latex-Amp, Latex-Anti PBP in control point reaction, forms Latex-Amp-PBP-HRP compound, is fixed on check point; Form Latex-Anti PBP-PBP-HRP compound and be fixed on control point.
B) positive milk: Penicillin G-PBP-HRP, owing to reacting, in the time flowing through check point, does not react with latex-Amp; And the PBP-HRP that part still exists in the mode of dissociating, with Latex-Amp reaction, forms Latex-Amp-PBP-HRP compound and is fixed on check point.Latex-Amp-PBP-HRP compound amount number, depend on the amount of Penicillin G in sample number, in sample, Penicillin G amount is many, Latex-Amp-PBP-HRP compound amount is few, otherwise otherwise.When the positive milk control point of flowing through, Latex-Anti PBP and Penicillin G-PBP-HRP and free PBP-HRP react, and form Latex-Anti PBP-Penicillin G-PBP-HRP compound and Latex-Anti PBP-PBP-HRP compound and are fixed on control point.In sample, Penicillin G concentration is higher, form Penicillin G-PBP-HRP compound amount more, free PBP-HRP amount is fewer, and in check point, Latex-Amp-PBP-HRP compound amount is few, but control point Latex-Anti PBP-Penicillin G-PBP-HRP is more.
3) washing: when reaction finishes, suction pumps arrives well, and a certain amount of washing lotion joins in washing lotion hole, suction pumps work, washing reaction film
4) colour developing: washing finishes, and adds a certain amount of nitrite ion in washing lotion hole, suction pumps work, colour developing 3min
5) detect judgement.Image identification system reads check point and control point gray-scale value automatically, and instrument internal arranges typical curve, according to the gray-scale value of check point and control point, and with reference to built-in typical curve, draws quantitative concentration value.
Milk in embodiment 1 detects, method and apparatus of the present invention is also applicable to other sample and test item of utilizing directed fast immune chromatographic detection method to detect, and the sample that in background technology, the kit of IDEXX company can detect and test item all can use method and apparatus of the present invention to detect.The sample that the present invention can detect mainly comprises: blood, serum, blood plasma, urine, chest ascites, hydrocrania, cells and supernatant, sputum, throat swab eluent, Fresh Milk, finished product milk, milk powder, animal vegetable tissue homogenate, animal blood, animal blood serum, animals urine and other body fluid of animal etc.The present invention can be for detection of people and animal blood sample, body fluid sample; in food liquid, animals and plants extract, detect pathogen, albumen, other molecules or chemical molecular etc.; step in detecting step and embodiment 1 is basic identical, only needs material and corresponding replacement washing lotion and nitrite ion in check point in replacement equipment, control point.
Claims (2)
1. the external unit quantitatively detecting for sample, it is characterized in that: at least comprise a floor component (11), floor component (11) is provided with bracket component (12), bracket component (12) below is provided with for holding kit and slidably passing in and out the board supporting plate (16) of external unit, is fixed with imbibition parts (13) and liquid feeding parts (14) on bracket component (12); Bracket component (12) is also fixed with barcode detection parts (19), image-detection component (15); Also comprise display unit (110) and control the control mainboard (112) of each parts.
2. the external unit quantitatively detecting for sample according to claim 1, is characterized in that: also comprise IC-card read-write parts (17), heater block (111) and fluorescent scanning detection part (18).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420049321.6U CN203672893U (en) | 2014-01-26 | 2014-01-26 | External device for quantitative detection of sample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420049321.6U CN203672893U (en) | 2014-01-26 | 2014-01-26 | External device for quantitative detection of sample |
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| Publication Number | Publication Date |
|---|---|
| CN203672893U true CN203672893U (en) | 2014-06-25 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201420049321.6U Withdrawn - After Issue CN203672893U (en) | 2014-01-26 | 2014-01-26 | External device for quantitative detection of sample |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103760338A (en) * | 2014-01-26 | 2014-04-30 | 杭州海士利生物科技有限公司 | Quantitative detection method by using reverse immune chromatography and kit and external equipment |
| CN104655605A (en) * | 2015-03-11 | 2015-05-27 | 厦门为正生物科技有限公司 | Detection card based intelligent detection liquid detector and method |
-
2014
- 2014-01-26 CN CN201420049321.6U patent/CN203672893U/en not_active Withdrawn - After Issue
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103760338A (en) * | 2014-01-26 | 2014-04-30 | 杭州海士利生物科技有限公司 | Quantitative detection method by using reverse immune chromatography and kit and external equipment |
| CN103760338B (en) * | 2014-01-26 | 2016-02-03 | 杭州海士利生物科技有限公司 | Apply quantitative detecting method and kit and the external unit of the reaction of reverse immunochromatography |
| CN104655605A (en) * | 2015-03-11 | 2015-05-27 | 厦门为正生物科技有限公司 | Detection card based intelligent detection liquid detector and method |
| CN104655605B (en) * | 2015-03-11 | 2017-03-15 | 厦门为正生物科技股份有限公司 | Detection liquid intelligent detection device and its method based on detection card |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20140625 Effective date of abandoning: 20160203 |
|
| C25 | Abandonment of patent right or utility model to avoid double patenting |