CN202083636U - Automatic detecting device for plurality of groups of chips - Google Patents
Automatic detecting device for plurality of groups of chips Download PDFInfo
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- CN202083636U CN202083636U CN2011201240540U CN201120124054U CN202083636U CN 202083636 U CN202083636 U CN 202083636U CN 2011201240540 U CN2011201240540 U CN 2011201240540U CN 201120124054 U CN201120124054 U CN 201120124054U CN 202083636 U CN202083636 U CN 202083636U
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- array chip
- load carrier
- determinand
- reaction
- robotization
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Abstract
The utility model relates to an automatic detecting device for a plurality of groups of chips, comprising a front processing area of objects to be detected, a purification extraction area, a bearing mechanism of a reverse transcription and labeling reaction area and a reaction and development area of a plurality of groups of the chips, an actuating, a temperature control mechanism, a reagent storage mechanism, an extraction mechanism, a magnetic field control mechanism, an image capture mechanism and an operating mechanism, wherein the actuating mechanism is used for shaking the bearing mechanism, the temperature control mechanism is used for controlling temperature of the bearing mechanism, the reagent storage mechanism is provided with a reagent placement area and a magnetic ball placement area, the extraction mechanism takes the needed reagent on the bearing mechanism and carries out operation relative to the objects to be detected, transferring and waste liquid discharge after reaction in all areas of the bearing mechanism, the magnetic field control mechanism carries out isolation and purification on the objects to be detected by matching the magnetic balls, the image capture mechanism is used for capturing the objects to be detected to carry out reaction with a plurality of groups of the chips and carrying out image processing after development, and the operating mechanism is used for control of all mechanisms, reaction image analysis and data display. In the utility model, the detection operation on the objects to be detected can be carried out by automatic mechanisms so as to achieve the effect of rapid detection and high accuracy.
Description
Technical field
The utility model relates to a kind of robotization array chip-detecting apparatus, refers to especially a kind ofly can carry out the processing of determinand and the operation of array chip by automatic mechanism, and reaches the pick-up unit of fast detecting and the high effect of precision.
Background technology
Generally in traditional molecular biosciences check and analysis flow process, the pre-treatment of biological clinical determinand is crucial operation steps with the program that concentrates extraction.In the traditional medicine applying detection, basic experimental technique, such as clinical determinand pre-treatment, DNA (deoxyribonucleic acid) (deoxyribonucleic acid, DNA) and RNA (ribonucleic acid) (ribonucleic acid, RNA) extraction and purifying are especially difficult, often waste the many time, and its complicated manually-operated and the loss of the easier increase determinand of step and the accuracy of detection.
Summary of the invention
Technical problem to be solved in the utility model is: at above-mentioned the deficiencies in the prior art, a kind of robotization array chip-detecting apparatus is provided, mode that can automatic mechanism is carried out the pre-treatment of determinand, comprise cracking, separation, purifying extraction and demarcation, reach operation with the array chip, finish with reaction and video picture after image capture and data analysis etc., and reach fast detecting and the high effect of precision.
In order to solve the problems of the technologies described above, the technical scheme that the utility model adopted is: a kind of robotization array chip-detecting apparatus, include: load carrier, its end face is provided with reaction of determinand pre-treatment district, purifying extraction section, reverse transcription and labeling response district and array chip and imaged sector at least, and determinand and array chip holds and the operation platform when detecting as determinand; Actuating mechanism is connected with load carrier, is used in the testing process load carrier be rocked or shakes; Temperature control mechanism is connected with load carrier, is used in the testing process load carrier be carried out temperature controlling; Reagent storage mechanism, include several reagent put the district, and magnetic bead put the district, determinand required reagent when reaction is provided; Extracts mechanism, be located between load carrier and the reagent storage mechanism, desirablely be used for required reagent, and determinand is in the transmission in load carrier Zhong Ge district in load carrier, and determinand finish react after the discharging of the waste liquid that produces; The magnetic field control gear is connected with load carrier, and the magnetic bead that can cooperate magnetic bead to put the district carries out purifying to determinand; Image capture mechanism, corresponding with array chip reaction and imaged sector, fechtable determinand and array chip react and video picture after image; And operating mechanism, with load carrier, actuating mechanism, temperature control mechanism, reagent storage mechanism, extract mechanism, magnetic field control gear, and image capture mechanism is connected, the control when can be used as the running of each mechanism, react image analysing computer and data presentation.
Be provided with the array type chip in described array chip reaction and the imaged sector, and have several bioprobes on each chip.Described actuating mechanism is a servo motor.Described extraction mechanism is a vacuum suction.Described image capture mechanism is a photosensory assembly.
Described operating mechanism comprises processing unit, input block, reaches output unit, and this input block and output unit are connected with processing unit respectively.But described processing unit is single-chip or is the programmed logic array.Described input block is several buttons.Described output unit is a display screen.
So, can cooperate above-mentioned step to reach the purpose of whole-course automation detecting operation by each mechanism of the present utility model, reduce the issuable error of traditional manual operation experiment, effectively set up Standard Operating Procedure, and can effectively quicken the carrying out of various reactions, and help to shorten detection time.
Description of drawings:
Fig. 1 is a basic framework calcspar of the present utility model.
Fig. 2 is a block schematic diagram of the present utility model.
Fig. 3 is the synoptic diagram of the utility model load carrier.
Label declaration:
Load carrier 1 determinand pre-treatment district 11
12 reverse transcriptions of purifying extraction section and labeling response district 13
Reaction of array chip and imaged sector 14 array type chips 141
Actuating mechanism 2 temperature control mechanisms 3
Reagent storage mechanism 4 reagent are put district 41
Magnetic bead is put district 42 and is extracted mechanism 5
Magnetic field control gear 6 image capture mechanisms 7
Operating mechanism 8 processing units 81
Embodiment:
See also Fig. 1 to shown in Figure 3, be respectively the synoptic diagram of basic framework calcspar of the present utility model, block schematic diagram of the present utility model and the utility model load carrier.As shown in the figure: the utility model is a kind of robotization array chip-detecting apparatus, and it comprises a load carrier 1, an actuating mechanism 2, a temperature control mechanism 3, reagent storage mechanism 4, extraction mechanism 5, magnetic field control gear 6, image capture mechanism 7 and operating mechanism 8 at least and constitutes.
The end face of the above-mentioned load carrier of carrying 1 is provided with a determinand pre-treatment district 11, a purifying extraction section 12, a reverse transcription and labeling response district 13 and array chip reaction and imaged sector 14 at least, holding and the operation platform when detecting as determinand, and be provided with array type chip 141 in this array chip reaction and the imaged sector 14, and have several bioprobes on each chip 141, video picture mode after the bioprobe reaction that determinand and array type chip 141 have is finished can be the ferment,chemical colour generation, but not as limit.
This actuating mechanism 2 is connected with load carrier 1, be used in the testing process load carrier 1 be rocked or shakes, and this actuating mechanism 2 can be servo motor, but not as limit.
This temperature control mechanism 3 is connected with load carrier 1, is used in the testing process load carrier 1 be carried out temperature controlling.
This reagent storage mechanism 4 include several reagent put district 41, and a magnetic bead put district 42, so that determinand required reagent when the reaction to be provided.
This extraction mechanism 5 is located between load carrier 1 and the reagent storage mechanism 4, can take required reagent on load carrier 1, and determinand is in the transmission in load carrier Zhong Ge district, and determinand is finished the discharging of the reaction back waste liquid that produces, and this extraction mechanism 5 can be vacuum suction, but not as limit.
This magnetic field control gear 6 is connected with load carrier 1, and the magnetic bead that can cooperate magnetic bead to put in the district 42 carries out the purifying extraction to determinand.
Reaction of this image capture mechanism 7 and array chip and imaged sector 14 are corresponding, fechtable determinand and array chip react and video picture after the image that produced, and this image capture mechanism 7 can be photosensory assembly, but not as limit.
This operating mechanism 8 is connected with load carrier 1, actuating mechanism 2, temperature control mechanism 3, reagent storage mechanism 4, extraction mechanism 5, magnetic field control gear 6 and image capture mechanism 7, control when can be used as the running of each mechanism, reaction image analysing computer and data presentation, and operating mechanism 8 includes a processing unit 81, an input block 82 that is connected with processing unit 81, reaches an output unit 83 that is connected with processing unit 81, wherein but this processing unit 81 can be single-chip or is the programmed logic array, this input block 82 can be several buttons, and this output unit 83 can be display screen.In this way, constitute a brand-new robotization array chip-detecting apparatus by said structure.
When the utility model during, include the following step at least in check and analysis:
One, determinand (as: blood) is delivered in the determinand pre-treatment district 11 of load carrier 1 and injects the cracking Treatment Solution simultaneously, and see through 3 pairs of determinand pre-treatments of temperature control mechanism district 11 and be heated to 35 ℃～80 ℃, and in the magnetic bead storing district 42 of reagent storage mechanism 4, magnetic bead and the required reagent of separation and purification are sent to determinand pre-treatment district 11 to extract mechanism 5, shake this load carrier 1 by actuating mechanism 2 with 100～200rpm again, above-mentioned determinand, magnetic bead and reaction reagent are mixed, so that magnetic bead effectively carries out the conjugation with determinand.
Two, the determinand of finishing joint in the determinand pre-treatment district 11 and magnetic bead are sent in the purifying extraction section 12 to extract mechanism 5, and by magnetic field control gear 6 absorption magnetic beads, and with determinand purifying in addition, remove the supernatant that does not contain magnetic bead in the purifying extraction section 12 to extract mechanism 5 this moment again.
Three, in reagent storing district 41, draw in the reaction solution adding purifying extraction section 12 to extract mechanism 5 again, close magnetic field control gear 6 simultaneously, magnetic bead is suspended, and shake this load carrier 1 with 100～200rpm by actuating mechanism 2, magnetic bead is fully mixed with reaction solution, allow determinand separate, and disengage biomolecule to be measured with magnetic bead.
Four, afterwards the solution that contains biomolecule to be measured in the purifying extraction section 12 is transmitted in reverse transcription and the labeling response district 13 to extract mechanism 5, in reagent storing district 41, draw reaction solution to extract mechanism 5 in reverse transcription and labeling response district 13 more in addition, and shake this load carrier 1 with 100～200rpm by actuating mechanism 2, and be heated to 35 ℃～80 ℃ by 3 pairs of reverse transcriptions of temperature control mechanism and labeling response district 13, use reverse transcription and the label labeling response of finishing biomolecule to be measured.
Five, be sent in reaction of array chip and the imaged sector 14 and react to extract mechanism 5 finishing reverse transcription and calibrated biomolecule to be measured in reverse transcription and the labeling response district 13, in reagent storing district 41, draw the number reaction solution to extract mechanism 5 in reaction of array chip and imaged sector 14, and shake this load carrier 1 with 100～200rpm by actuating mechanism 2, and be heated to 35 ℃～80 ℃ by reaction of temperature control mechanism 3 logarithm core assembly sheets and imaged sector 14, use by the bioprobe on the array type chip 141 and do the selectivity combination, carry out the video picture reaction with required reaction reagent again.
Six, the bioprobe that is had by the reaction of image capture mechanism 7 logarithm core assembly sheets and imaged sector 14 acquisition biomolecule to be measured and array type chip 141 again carries out the image that produces after association reaction and the video picture, and cooperate processing unit 81 to carry out the interpretation of image with output unit 83 by the input block 82 of operating mechanism 8, or relevant transmission and demonstration, and can be by 7 picked image of image capture mechanism, and carry out analysis and distinguishing, promptly finish the detection of specific biological molecules in the determinand.
In this way, can cooperate above-mentioned step to reach the purpose of whole-course automation detecting operation by each mechanism of the present utility model, reduce the issuable error of traditional manual operation experiment, effectively set up Standard Operating Procedure, and can effectively quicken the carrying out of various reactions, and help to shorten detection time.
In sum, the utility model robotization array chip-detecting apparatus can effectively improve the various shortcoming of prior art, the detecting operation that can automatic mechanism carries out determinand, and reach fast detecting and the high effect of precision, and then make the utility model can produce more progressive, more practical, more meet the consumer use the institute must, really meet the important document of utility application, proposed patented claim in accordance with the law.
Only the above only is preferred embodiment of the present utility model, when not limiting the scope that the utility model is implemented with this; So all simple equivalent of doing according to the utility model claim and description change and modify, all should still belong in the scope that the utility model patent contains.
Claims (9)
1. robotization array chip-detecting apparatus is characterized in that: include:
Load carrier, its end face are provided with reaction of determinand pre-treatment district, purifying extraction section, reverse transcription and labeling response district and array chip and imaged sector at least, and determinand and array chip holds and the operation platform when detecting as determinand;
Actuating mechanism is connected with load carrier, in testing process load carrier is rocked or shakes;
Temperature control mechanism is connected with load carrier, in testing process load carrier is carried out temperature controlling;
Reagent storage mechanism, include several reagent put the district, and magnetic bead put the district, determinand required reagent when reaction is provided;
Extracts mechanism, be located between load carrier and the reagent storage mechanism, desirablely be used for required reagent, and determinand is in the transmission in load carrier Zhong Ge district in load carrier, and determinand finish react after the discharging of the waste liquid that produces;
The magnetic field control gear is connected with load carrier, and the magnetic bead that can cooperate magnetic bead to put the district carries out purifying to determinand;
Image capture mechanism, corresponding with array chip reaction and imaged sector, fechtable determinand and array chip react and video picture after image; And
Operating mechanism, with load carrier, actuating mechanism, temperature control mechanism, reagent storage mechanism, extract mechanism, magnetic field control gear, and image capture mechanism is connected, the control when can be used as the running of each mechanism, react image analysing computer and data presentation.
2. robotization array chip-detecting apparatus as claimed in claim 1 is characterized in that: be provided with the array type chip in described array chip reaction and the imaged sector, and have several bioprobes on each chip.
3. robotization array chip-detecting apparatus as claimed in claim 1 is characterized in that: described actuating mechanism is a servo motor.
4. robotization array chip-detecting apparatus as claimed in claim 1 is characterized in that: described extraction mechanism is a vacuum suction.
5. robotization array chip-detecting apparatus as claimed in claim 1 is characterized in that: described image capture mechanism is a photosensory assembly.
6. robotization array chip-detecting apparatus as claimed in claim 1 is characterized in that: described operating mechanism comprises processing unit, input block, reaches output unit, and this input block and output unit are connected with processing unit respectively.
7. robotization array chip-detecting apparatus as claimed in claim 6 is characterized in that: but described processing unit is single-chip or is the programmed logic array.
8. robotization array chip-detecting apparatus as claimed in claim 6, it is characterized in that: described input block is several buttons.
9. robotization array chip-detecting apparatus as claimed in claim 6 is characterized in that: described output unit is a display screen.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011201240540U CN202083636U (en) | 2011-04-25 | 2011-04-25 | Automatic detecting device for plurality of groups of chips |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011201240540U CN202083636U (en) | 2011-04-25 | 2011-04-25 | Automatic detecting device for plurality of groups of chips |
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| CN202083636U true CN202083636U (en) | 2011-12-21 |
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| CN2011201240540U Expired - Fee Related CN202083636U (en) | 2011-04-25 | 2011-04-25 | Automatic detecting device for plurality of groups of chips |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102600966A (en) * | 2012-03-06 | 2012-07-25 | 南昌大学 | Simple magnetic bead separating device |
| CN102768285A (en) * | 2011-05-05 | 2012-11-07 | 辅英科技大学附设医院 | Automatic array chip detection device |
| CN108795686A (en) * | 2017-05-03 | 2018-11-13 | 莱弗斯基因股份有限公司 | Culture apparatus with rotation system and culture systems |
-
2011
- 2011-04-25 CN CN2011201240540U patent/CN202083636U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102768285A (en) * | 2011-05-05 | 2012-11-07 | 辅英科技大学附设医院 | Automatic array chip detection device |
| CN102600966A (en) * | 2012-03-06 | 2012-07-25 | 南昌大学 | Simple magnetic bead separating device |
| CN108795686A (en) * | 2017-05-03 | 2018-11-13 | 莱弗斯基因股份有限公司 | Culture apparatus with rotation system and culture systems |
| CN108795686B (en) * | 2017-05-03 | 2022-09-23 | 莱弗斯基因股份有限公司 | Culture instrument with rotating system and culture system |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20111221 Termination date: 20190425 |