CN110346585A - A kind of on piece laboratory testing method and system - Google Patents
A kind of on piece laboratory testing method and system Download PDFInfo
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- CN110346585A CN110346585A CN201910623398.7A CN201910623398A CN110346585A CN 110346585 A CN110346585 A CN 110346585A CN 201910623398 A CN201910623398 A CN 201910623398A CN 110346585 A CN110346585 A CN 110346585A
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- 238000006243 chemical reaction Methods 0.000 claims abstract description 207
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- 238000004140 cleaning Methods 0.000 claims abstract description 39
- 238000001514 detection method Methods 0.000 claims abstract description 21
- 239000000758 substrate Substances 0.000 claims abstract description 11
- 238000000034 method Methods 0.000 claims description 30
- 238000007781 pre-processing Methods 0.000 claims description 22
- 230000035807 sensation Effects 0.000 claims description 22
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- 239000000376 reactant Substances 0.000 claims description 9
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 claims description 7
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00178—Special arrangements of analysers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00346—Heating or cooling arrangements
- G01N2035/00356—Holding samples at elevated temperature (incubation)
Abstract
On piece laboratory testing method disclosed by the invention provides constant reaction temperature for reaction zone, and the liquid in drive response area circulates at least two reactor when sample is full of reaction zone；The cleaning solution controlled in reagent area flows into reaction zone, and for cleaning remaining sample in reaction zone, the enzymic-labelled antibody controlled in reagent area flows into reaction zone, and constant reaction temperature is provided for reaction zone；The cleaning solution controlled in reagent area flows into reaction zone, and for cleaning remaining enzymic-labelled antibody in reaction zone, the substrate controlled in reagent area flows into reaction zone, and constant reaction temperature is provided for reaction zone；The light intensity data in each reactor is obtained respectively, and the content of determinand in each reactor is determined according to the light intensity data in each reactor.In this way, the liquid in driving runner circulates at least two reactors, each reactor can come into full contact with the sample in runner, so as to reduce sample size when the multinomial detection on piece laboratory.
Description
Technical field
The present invention relates to detection technique field more particularly to a kind of on piece laboratory testing method and system.
Background technique
On piece laboratory technique is the bases such as biology, chemistry, the sample preparation of medical analysis process, reaction, separation, detection
This operating unit is integrated on the chip of one piece of micro-meter scale, is automatically performed analysis overall process.Since it is in biology, chemistry, doctor
The great potential in etc. fields has been developed as the friendship of the subjects such as biology, chemistry, medicine, fluid, electronics, material, a machinery
The brand-new research field of fork.
Currently, a sample can typically be only used to a detection, when needing to carry out multinomial detection, then need to obtain more parts
Sample is detected respectively.However, a large amount of samplings may impact target, certain situations even can not largely obtain sample
This.
As it can be seen that in the prior art, sample size when how to reduce the multinomial detection on piece laboratory becomes skill urgently to be resolved
Art problem.
Summary of the invention
In view of this, the present invention proposes a kind of on piece laboratory testing method and system, to solve the above technical problems.
Firstly, to achieve the above object, the present invention proposes a kind of on piece laboratory testing method, it is applied to an on piece and tests
Room detection system, the on piece laboratory testing system include on piece laboratory and on piece laboratory testing device, and described
Upper laboratory includes reagent area and reaction zone, and the reaction zone includes at least two reactors being sequentially communicated, and different is anti-
Device inner surface is answered to adhere to different reactants, which comprises
When sample is full of the reaction zone in the on piece laboratory, constant reaction temperature is provided for the reaction zone, and
The liquid in the reaction zone is driven to circulate at least two reactor；
The cleaning solution controlled in the reagent area flows into the reaction zone, for cleaning remaining sample in the reaction zone
This, the enzymic-labelled antibody controlled in the reagent area flows into the reaction zone, and constant reaction temperature is provided for the reaction zone
Degree；
The cleaning solution controlled in the reagent area flows into the reaction zone, for cleaning remaining enzyme mark in the reaction zone
Remember antibody, the substrate controlled in the reagent area flows into the reaction zone, and constant reaction temperature is provided for the reaction zone；
The light intensity data in each reactor is obtained respectively, and is determined according to the light intensity data in each reactor each anti-
Answer the content of determinand in device.
It is optionally, described that constant reaction temperature is provided for the reaction zone, comprising:
To the thermostat module of the on piece laboratory testing device, the thermostat module is institute in the mobile on piece laboratory
It states reaction zone and constant reaction temperature is provided.
It is optionally, described that constant reaction temperature is provided for the reaction zone, comprising:
Constant reaction temperature is provided for the reaction zone, and drives the liquid in the reaction zone described at least two
It is circulated in reactor.
Optionally, the entrance and exit of the reaction zone passes through runner respectively and connect with first pump housing, the thermostat
Including the first pump head, the liquid in the driving reaction zone circulates at least two reactor, comprising:
First pump housing described in first pump head drive is controlled, so that the liquid in the reaction zone is described at least two
It is circulated in reactor.
Optionally, the light intensity data obtained in each reactor respectively, comprising:
The light sensation module in the on piece laboratory to the on piece laboratory testing device is moved, and controls the light sensation mould
Block obtains the light sensation data in each reactor respectively.
Optionally, the method also includes:
Before sample flows into the reaction zone, pre-treatment is carried out to sample.
Optionally, the on piece laboratory further includes sample entrance port and is arranged in the sample entrance port and the reaction zone
Between pre-treatment area, the pre-treatment area include second pump housing, the on piece laboratory testing device includes pre-processing module,
The pre-processing module includes plugging device and the second pump head, described to carry out pre-treatment to sample, comprising:
To the pre-processing module, the plugging device blocks the hair in the on piece laboratory in the mobile on piece laboratory
Thin blood sampling tube inlet；
Second pump housing described in second pump head drive is controlled to supply to the capillary heparin tube in the on piece laboratory.
Optionally, the pre-processing module further includes the first liquid sensor, when the on piece laboratory be moved to it is described
When at pre-processing module position, first liquid sensor is located at the exit position of the reaction zone, and the method is also wrapped
It includes:
The reaction zone whether sample is full of the on piece laboratory is detected by first liquid sensor.
Optionally, the on piece laboratory testing device includes traction device, the movement on piece laboratory, packet
It includes:
It controls the traction device and draws the on piece laboratory movement.
The present invention also provides a kind of on piece laboratory testing system, the on piece laboratory testing system includes on piece experiment
Room and on piece laboratory testing device, the on piece laboratory include reagent area and reaction zone, the reaction zone include according to
At least two reactors of secondary connection, different reactor inner surfaces adhere to different reactants, the on piece laboratory testing
Device includes memory, at least one processor and is stored on the memory and can execute at least one described processor
At least one program, at least one described program realizes the step in above-mentioned method when being executed by least one described processor
Suddenly.
Compared to the prior art, on piece laboratory testing method proposed by the invention is when sample is full of on piece experiment
When the reaction zone of room, constant reaction temperature is provided for the reaction zone, and drive liquid in the reaction zone it is described extremely
It is circulated in few two reactors；The cleaning solution controlled in the reagent area flows into the reaction zone, described anti-for cleaning
Remaining sample in area is answered, the enzymic-labelled antibody in the reagent area is controlled and flows into the reaction zone, and mentioned for the reaction zone
For constant reaction temperature；The cleaning solution controlled in the reagent area flows into the reaction zone, for cleaning in the reaction zone
Remaining enzymic-labelled antibody controls substrate in the reagent area and flows into the reaction zone, and provides for the reaction zone constant
Reaction temperature；The light intensity data in each reactor is obtained respectively, and is determined often according to the light intensity data in each reactor
The content of determinand in a reactor.In this way, the liquid on piece laboratory testing method driving runner provided by the invention exists
It being circulated at least two reactors of the reaction zone, each reactor can come into full contact with the sample in runner, from
And sample size when the multinomial detection on piece laboratory can be reduced.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of on piece laboratory testing system provided in an embodiment of the present invention；
Fig. 2 is a kind of one of the connection schematic diagram of on piece laboratory testing device provided in an embodiment of the present invention；
Fig. 3 is a kind of connection schematic diagram on piece laboratory provided in an embodiment of the present invention；
Fig. 4 is a kind of flow diagram of on piece laboratory testing method provided in an embodiment of the present invention；
Fig. 5 is a kind of structural schematic diagram of thermostat module provided in an embodiment of the present invention；
Fig. 6 is a kind of structural schematic diagram of crowded liquid module provided in an embodiment of the present invention；
Fig. 7 is a kind of structural schematic diagram of light sensation module provided in an embodiment of the present invention；
Fig. 8 is the two of the connection schematic diagram of on piece laboratory testing device provided in an embodiment of the present invention；
Fig. 9 is a kind of structural schematic diagram of pre-processing module provided in an embodiment of the present invention；
Figure 10 is a kind of structural schematic diagram of on piece laboratory testing device provided in an embodiment of the present invention.
The embodiments will be further described with reference to the accompanying drawings for the realization, the function and the advantages of the object of the present invention.
Specific embodiment
It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not intended to limit the present invention.
Referring to Fig. 1, Fig. 1 is a kind of structural schematic diagram of on piece laboratory testing system provided in an embodiment of the present invention,
As shown in Figure 1, the on piece laboratory testing system 100 includes on piece laboratory testing device 200 and on piece laboratory
300。
Referring to Figure 2 together, Fig. 2 be on piece laboratory testing device provided in an embodiment of the present invention connection schematic diagram it
One, as shown in Fig. 2, the on piece laboratory testing device 200 is including shell 10, traction device 20 and is set to the shell
Thermostat module 30, crowded liquid module 40 and light sensation module 50 inside 10.
It is the connection schematic diagram on piece laboratory provided in an embodiment of the present invention also referring to Fig. 3, Fig. 3, such as Fig. 3 institute
Show, the on piece laboratory 300 includes sample entrance port 301, reagent area 302, reaction zone 303 and waste liquid pool 304, the reaction
The entrance in area 303 is connect by runner 305 with the sample entrance port 301 and the reagent area 302, the reaction zone 303
Outlet is connect by runner 305 with the waste liquid pool 304.The reaction zone 303 includes at least two reactors being sequentially communicated
3031, i.e., described at least two reactor 3031 is serially connected, wherein different 3031 inner surfaces of reactor adheres to different anti-
Object is answered, for carrying out the detection of disparity items.
Referring to Fig. 4, Fig. 4 is a kind of flow diagram of on piece laboratory testing method provided in an embodiment of the present invention,
The on piece laboratory testing method is applied to laboratory testing system in sheet above, as shown in figure 4, the method includes as follows
Step:
Step 401, when sample be full of the on piece laboratory reaction zone when, constant reaction is provided for the reaction zone
Temperature, and the liquid in the reaction zone is driven to circulate at least two reactor.
In the step, when sample is full of the reaction zone in the on piece laboratory, provided for the reaction zone constant anti-
Temperature is answered, and the liquid in the reaction zone is driven to circulate at least two reactor.Under normal conditions, described
On piece laboratory is stored in 2-8 degrees Celsius of environment, and when detection needs to simulate the body temperature environment of human body, the on piece laboratory
Detection method provides constant reaction temperature for the reaction zone.
Specifically, as shown in Fig. 2, the on piece laboratory testing device 200 includes thermostat module 30, the thermostat module
30 thermostat modules are used to that the on piece laboratory to be heated in normal body temperature's range (such as 37 degrees Celsius) and be kept.
Please refer to fig. 5, Fig. 5 is the structural schematic diagram of thermostat module 30 provided in an embodiment of the present invention, as shown in figure 5, the perseverance
Warm module 30 includes thermostat 31, and the thermostat 31 may include a fever tablet and temperature-detecting device, the method root
The fever tablet, which is controlled, according to the temperature of temperature-detecting device detection starts or stop fever.
The method can be driven by the liquid in the on piece laboratory testing in the pump head of at least two device
The dynamic pump housing connecting with the reaction zone, realizes and the liquid in the reaction zone is driven to recycle at least two reactor
Flowing.Specifically, as shown in figure 3, the entrance and exit of the reaction zone 303 passes through runner 305 and first pump housing 3032 respectively
Connection, the thermostat module 30 further include the first pump head 32, and the method controls first pump head 32 and drives first pump
Body 3032, so that the liquid in the reaction zone be driven to circulate at least two reactor.
By taking antigen or antibody test as an example, when sample circulates at least two reactor, sample and every
The reactant of a reactor inner surface attachment comes into full contact with, the reactant reaction with reactor surface attachment, to be attached to anti-
Answer device surface, the determinand can be antigen or antibody, and correspondingly, the reactant can be antibody or antigen, can
With understanding, when determinand is antigen, corresponding reactant is can be with the antibody of the antigen binding；When determinand is
When antibody, corresponding reactant is can be with the antigen in conjunction with the antibody.For antigen or antibody test project, the sample
Originally the circulation time at least two reactor is preset duration, such as can be 10 minutes.
Cleaning solution in step 402, the control reagent area flows into the reaction zone, residual in the reaction zone for cleaning
The sample stayed controls enzymic-labelled antibody in the reagent area and flows into the reaction zone, and provides for the reaction zone constant
Reaction temperature.
In the step, the cleaning solution controlled in the reagent area flows into the reaction zone, for cleaning in the reaction zone
Sample (such as cleaning the antigen or antibody being not associated in the reaction zone) not in conjunction with the reactant of reactor surface.?
It cleaning after the sample being not associated in the reaction zone, the enzymic-labelled antibody controlled in the reagent area flows into the reaction zone,
And constant reaction temperature is provided for the reaction zone.
Specifically, referring to Fig. 3, the reagent area 302 may include at least three reagent packets 3021, the method can be with
At the mobile on piece laboratory to the crowded liquid module 40, and controls the crowded liquid module 40 and squeeze one of reagent packet
3021, so that the liquid in the reagent packet 3021 flows into the reaction zone.
Referring to Figure 6 together, Fig. 6 is the structural schematic diagram of crowded liquid module provided in an embodiment of the present invention, as shown in fig. 6,
The crowded liquid module 40 includes opener 41 and extrusion device 42.Each reagent packet 3021 enters with the reaction zone 303
It is provided with valve 3022 between mouthful, the opener 41 opens the valve of one of reagent packet, the extrusion device 42
The reagent packet is squeezed, the liquid in the reagent packet flows into the reaction zone in the on piece laboratory.It is understood that above-mentioned
The concrete mode that the reaction zone in the on piece laboratory is flowed into for the liquid in driving reagent packet, when the not phase of the liquid in reagent packet
Meanwhile the liquid flowed into the reaction zone is not identical.
By taking detection project is antigen or antibody test as an example, the reaction zone 303 include 6 reactors 3031 (such as
3031A, 3031B, 3031C, 3031D, 3031E, 3031F in Fig. 3) reagent area 302 include 6 each reagent packets (such as
3021A, 3021B, 3021C, 3021D, 3021E, 3021F in Fig. 3) for, wherein the liquid in reagent packet 3021A is clear
Washing lotion, the liquid in reagent packet 3021B are enzymic-labelled antibody, and the liquid in reagent packet 3021C, 3021D, 3021E is cleaning
Liquid, the liquid in reagent packet 3021F are substrate, the corresponding valve of each reagent packet be respectively 3022A, 3022B, 3022C,
3022D、3022E、3022F。
Cleaning solution in the control reagent area, which flows into the reaction zone, can specifically include: the mobile on piece is real
It tests at room to the crowded liquid module 40, controls the 41 Open valve 3022A of opener, and control the extrusion device 42 and squeeze
The reagent packet 3021A is pressed, so that the cleaning solution in the reagent packet 3021A flows into the reaction zone.
It may include: that the mobile on piece is real that enzymic-labelled antibody in the control reagent area, which flows into the reaction zone,
It tests at room to the crowded liquid module 40, controls the 41 Open valve 3022B of opener, and control the extrusion device 42 and squeeze
The reagent packet 3021B is pressed, so that the enzymic-labelled antibody in the reagent packet 3021B flows into the reaction zone.It is understood that
It is that, when carrying out multinomial detection, the liquid for including is mixing enzymic-labelled antibody, the mixed enzyme mark in the reagent packet 3021B
It include all enzymic-labelled antibodies for realizing the multinomial detection in note antibody.
It is described to provide constant reaction temperature for the reaction zone detailed description has been carried out in step 401, herein
It repeats no more.
Cleaning solution in step 403, the control reagent area flows into the reaction zone, residual in the reaction zone for cleaning
The enzymic-labelled antibody stayed controls substrate in the reagent area and flows into the reaction zone, and provides for the reaction zone constant
Reaction temperature.
In the step, the method controls the cleaning solution in the reagent area and flows into the reaction zone, described for cleaning
Remaining enzymic-labelled antibody in reaction zone controls the substrate in the reagent area and flows into the reaction zone, and is the reaction zone
Constant reaction temperature is provided.Liquid in the control reagent area flows into the reaction zone and provides for reaction zone constant
Detailed description has been carried out in reaction temperature above, and details are not described herein again.
Specifically, at the mobile on piece laboratory to the crowded liquid module 40 of the method, the opener is controlled
41 Open valve 3022C, and control the extrusion device 42 and squeeze the reagent packet 3021C, so that in the reagent packet 3021C
Cleaning solution flow into the reaction zone.It should be noted that if remaining enzymic-labelled antibody is more in reaction zone, it is easy to detection
As a result large effect is caused, therefore, the capacity that the reagent packet 3021C can be set is bigger than other reagent inclusion quantities, uses
More cleaning solution cleans the reaction zone.In view of external compression device can only reagent packet to same specification carry out
It squeezes, it can be equal by pouring into cleaning agent in continuous multiple reagent packets, such as in reagent packet 3021C, 3021D and 3021E
Cleaning agent is poured into, the method successively squeezes the cleaning agent in described reagent packet 3021C, 3021D and 3021E and flows into the reaction
Qu Zhong.
After cleaning the reaction zone using cleaning solution, the method controls 41 Open valve of opener
3022F, and control the extrusion device 42 and squeeze the reagent packet 3021F, so that the substrate in the reagent packet 3021F flows into
The reaction zone.
Step 404 obtains light intensity data in each reactor respectively, and true according to the light intensity data in each reactor
The content of determinand in fixed each reactor.
In the step, the method obtains the light intensity data in each reactor respectively, and according in each reactor
Light intensity data determines the content of determinand in each reactor.The method can move the on piece laboratory to the on piece
At the light sensation module 50 of laboratory testing device, and controls the light sensation module 50 and obtain light sensation number in each reactor respectively
According to.In order to avoid influencing each other in adjacent reactor, in some embodiments, optical isolation dress can be set between each reactor
It sets.
In the present embodiment, the on piece laboratory testing method when sample be full of the on piece laboratory reaction zone when,
Constant reaction temperature is provided for the reaction zone, and drives the liquid in the reaction zone at least two reactor
It circulates；The cleaning solution controlled in the reagent area flows into the reaction zone, for cleaning remaining sample in the reaction zone
This, the enzymic-labelled antibody controlled in the reagent area flows into the reaction zone, and constant reaction temperature is provided for the reaction zone
Degree；The cleaning solution controlled in the reagent area flows into the reaction zone, anti-for cleaning remaining enzyme label in the reaction zone
Body, the substrate controlled in the reagent area flows into the reaction zone, and constant reaction temperature is provided for the reaction zone；Respectively
The light intensity data in each reactor is obtained, and determinand in each reactor is determined according to the light intensity data in each reactor
Content.In this way, liquid on piece laboratory testing method provided by the invention driving runner the reaction zone at least
It is circulated in two reactors, each reactor can come into full contact with the sample in runner, so as to reduce on piece reality
Test sample size when the multinomial detection in room.
It is optionally, described that constant reaction temperature is provided for the reaction zone, comprising:
To the thermostat module of the on piece laboratory testing device, the thermostat module is institute in the mobile on piece laboratory
It states reaction zone and constant reaction temperature is provided.
It is optionally, described that constant reaction temperature is provided for the reaction zone, comprising:
Constant reaction temperature is provided for the reaction zone, and drives the liquid in the reaction zone described at least two
It is circulated in reactor.
In the embodiment, while the method provides constant reaction temperature for the reaction zone, the reaction is driven
Liquid in area circulates at least two reactor, so that the inner surface of liquid and each reactor sufficiently connects
Touching improves reaction efficiency.
Optionally, the entrance and exit of the reaction zone passes through runner respectively and connect with first pump housing, the thermostat
Including the first pump head, the liquid in the driving reaction zone circulates at least two reactor, comprising:
First pump housing described in first pump head drive is controlled, so that the liquid in the reaction zone is described at least two
It is circulated in reactor.
Optionally, the light intensity data obtained in each reactor respectively, comprising:
The light sensation module in the on piece laboratory to the on piece laboratory testing device is moved, and controls the light sensation mould
Block obtains the light sensation data in each reactor respectively.
In the embodiment, the light sensation mould in the mobile on piece laboratory of the method to the on piece laboratory testing device
Block, and control the light sensation module and obtain light sensation data in each reactor respectively.Referring to Fig. 7, Fig. 7 is implementation of the present invention
A kind of structural schematic diagram for light sensation module that example provides, as shown in fig. 7, the light sensation module 50 includes photodetector 51, when
When the on piece laboratory is moved at 50 position of light sensation module, the photodetector 51 is directed at the on piece laboratory
Reaction zone, and obtain the light intensity data of each reactor.
Since environment light is easy to cause very big influence to testing result.In some embodiments, the light sensation module 50 is also
Including the brightness room 52 being arranged between the photodetector 51 and the traction device 20, when the on piece laboratory 300
When being moved at 50 position of light sensation module, the brightness room 52 is located at the photodetector 51 and the on piece laboratory
Between 300, for being the 200 shielding environment light of on piece laboratory, reduce influence of the environment light to testing result, to improve
Detection accuracy.The brightness room 52 is ring light darkroom, such as " O " figure, when the on piece laboratory 300 is moved to the light
When feeling at 50 position of module, it is interference fitted between the brightness room 52 and the on piece laboratory 300.
Optionally, the method also includes:
Before sample flows into the reaction zone, pre-treatment is carried out to sample.
Optionally, the on piece laboratory further includes sample entrance port and is arranged in the sample entrance port and the reaction zone
Between pre-treatment area, the pre-treatment area include second pump housing, the on piece laboratory testing device includes pre-processing module,
The pre-processing module includes plugging device and the second pump head, described to carry out pre-treatment to sample, comprising:
To the pre-processing module, the plugging device blocks the hair in the on piece laboratory in the mobile on piece laboratory
Thin blood sampling tube inlet；
Second pump housing described in second pump head drive is controlled to supply to the capillary heparin tube in the on piece laboratory.
Fig. 8 and Fig. 9 are please referred to, in the embodiment, the on piece laboratory testing device further includes pre-processing module 60, institute
Stating pre-processing module 60 includes plugging device 61 and the second pump head 62.The on piece laboratory 300 further includes pre-treatment area
306, the pre-treatment area 306 is arranged between the sample entrance port 301 and the reaction zone 303, described in the method control
Pre-processing module 60 and the pre-treatment area 306 cooperate, and realize and carry out pre-treatment to sample.It wraps in the pre-treatment area 306
Filter 3061 is included, the filter 3061 is arranged between the sample entrance port 301 and the entrance of the reaction zone 303, uses
It is filtered in sample.In this way, the pre-treatment area 306 can also be filtered sample, no when the sample size of acquisition is less
It needs through preparatory centrifugally operated, to avoid the waste of sample.In addition, compared to centrifugally operated, in the embodiment before
Treatment region 306 can effectively reduce the time to sample filtering, to accelerate to detect speed.
When the traction device 20 drives the on piece laboratory 300 to be moved at 60 position of pre-processing module,
The plugging device 61 blocks the entrance of the capillary heparin tube 3064 in the on piece laboratory 300, second pump head 62 and institute
Second pump housing 3063 for stating on piece laboratory 300 connects and drives second pump housing 3063 to the on piece laboratory 300
Capillary heparin tube 3064 supplies.In such manner, it is possible to seal the entrance of the capillary heparin tube 3064, second pump housing 3063 is avoided
Sample is pumped out from the inlet of the capillary heparin tube 3064 when work.
Optionally, the pre-processing module further includes the first liquid sensor, when the on piece laboratory be moved to it is described
When at pre-processing module position, first liquid sensor is located at the exit position of the reaction zone, and the method is also wrapped
It includes:
The reaction zone whether sample is full of the on piece laboratory is detected by first liquid sensor.
In the embodiment, the pre-processing module 60 further includes the first liquid sensor 63, when the on piece laboratory 300
When being moved at 60 position of front processor module, first liquid sensor 63 is located at the outlet of the reaction zone 303
At position, the method detects the outlet position whether sample reaches the reaction zone 303 by first liquid sensor 63
Set place.When sample reaches the exit position of the reaction zone 303, then illustrate that liquid sample has filled up the reaction zone 303,
More samples are not needed to flow into.In some embodiments of the invention, the front processor module 60 can also include second liquid
Sensor 64, when the on piece laboratory 300 is moved at 60 position of pre-processing module, the second liquid sensor
64 are located at the entry position of the reaction zone 303, and the entry position of the reaction zone 303 whether is reached for detecting liquid
Place.In this way, user is facilitated to understand the sample size of injection in time.
Optionally, the on piece laboratory testing device includes traction device, the movement on piece laboratory, packet
It includes:
It controls the traction device and draws the on piece laboratory movement.
In the embodiment, the method controls the traction device 20 and draws the on piece laboratory movement.The traction
Device 20 may include motor and conveyer belt, and the conveyer belt moves under the driving of the motor.
In the embodiment of the present invention, each module of the on piece laboratory testing device 200 setting alignment sensor (such as
Alignment sensor 33, alignment sensor 43, alignment sensor 53, alignment sensor 65 of the Fig. 5 into Fig. 9), first pump housing
3032 corresponding positions are provided with the first grating hole 30321, and the corresponding position of each reagent packet 3021 is provided with the second light
Gate hole 3024, the corresponding position of each reactor 3031 are provided with third grating hole 3033, set at second pump housing 3063
It is equipped with the 4th grating hole 30631.The method passes through the grating hole phase interworking of alignment sensor and the on piece laboratory 300
It closes, realizes the accurate positionin to the on piece laboratory 300.
The present invention also provides a kind of on piece laboratory testing system, the on piece laboratory testing system includes on piece experiment
Room and on piece laboratory testing device, referring to Fig. 10, Figure 10 is a kind of on piece laboratory inspection provided in an embodiment of the present invention
The structural schematic diagram of device is surveyed, as shown in Figure 10, the on piece laboratory testing device 1000 includes memory 1001, at least one
A processor 1002 and be stored on the memory 1001 and can be executed at least one described processor 1002 at least one
A program, at least one described program realize the step in above-mentioned method when being executed by least one described processor 1002.
In the prior art, reaction zone addition sample needs user to be operated manually, and is easy to produce human error.The present invention
The on piece laboratory testing method and system that embodiment provides pass through the voluntarily liquid relief of the pump housing built in pump head drive on piece laboratory,
It ensure that liquid relief precision and liquid relief consistency, can be improved the precision and repeatability of micro-fluidic product.
Present invention is described by taking actually detected project as an example below, and the on piece laboratory testing method can be used in
Heart injury marker detection heart infarction three: cardiac muscle troponin I (cTnI), creatine kinase isozyme (CK-MB), myoglobins
(Myo).Three reactors on piece laboratory are selected, are coated with cTnI, CK-MB, Myo first antibody respectively in its inner surface.
It is mixed that cTnI enzyme-labeled secondary antibody, CK-MB enzyme-labeled secondary antibody and Myo enzyme-labeled secondary antibody are injected in reagent packet 3021B
Close liquid.
Assuming that reactor 3031A is coated with cTnI first antibody, reactor 3031B is coated with CK-MB first antibody, reactor
3031C is coated with Myo first antibody.It is completed when blood plasma is incubated in reaction zone, reactor 3031A Surface Creation " cTnI first antibody+
CTnI " compound.Reactor 3031B Surface Creation " CK-MB first antibody+CK-MB " compound.The surface reactor 3031C is raw
At " Myo first antibody+Myo " compound.Remaining free unbonded composition, clears out of reaction by the cleaning solution of reagent packet 3021A
Device.Then the enzyme-labeled secondary antibody hybrid working liquid of reagent packet 3021B injects reactor.
It is completed when enzyme-labeled secondary antibody hybrid working liquid is incubated in reaction zone, reactor 3031A Surface Creation " cTnI the
One antibody+cTnI+ (cTnI secondary antibody ++ enzyme) " compound.Reactor 3031B Surface Creation " CK-MB first antibody+CK-MB
+ (CK-MB secondary antibody ++ enzyme) " compound.Reactor 3031C Surface Creation " Myo first antibody+Myo+ (Myo secondary antibody+
+ enzyme) " compound.Remaining free unbonded composition, clears out of reaction by the cleaning solution in reagent packet 3021C, 3021D and 3021E
Device.Then the substrate in reagent packet 3021F injects reactor.
Substrate is reacted with enzyme, and luminous intensity is related to the quantity of enzyme, and the quantity of enzyme is related to the quantity of marker,
The concentration with luminous intensity of so marker have functional relation.Light intensity data pass corresponding with marker concentration can be stored in advance
It is table, the method passes through the light intensity detected can find corresponding marker concentration from the mapping table.
The above is only a preferred embodiment of the present invention, is not intended to limit the scope of the invention, all to utilize this hair
Equivalent structure or equivalent flow shift made by bright specification and accompanying drawing content is applied directly or indirectly in other relevant skills
Art field, is included within the scope of the present invention.
Claims (10)
1. a kind of on piece laboratory testing method is applied to an on piece laboratory testing system, which is characterized in that the on piece is real
Testing room detection system includes on piece laboratory and on piece laboratory testing device, the on piece laboratory include reagent area and
Reaction zone, the reaction zone include at least two reactors being sequentially communicated, and different reactor inner surfaces adheres to different anti-
Answer object, which comprises
When sample is full of the reaction zone in the on piece laboratory, constant reaction temperature is provided for the reaction zone, and drive
Liquid in the reaction zone circulates at least two reactor；
The cleaning solution controlled in the reagent area flows into the reaction zone, for cleaning remaining sample in the reaction zone, controls
The enzymic-labelled antibody made in the reagent area flows into the reaction zone, and constant reaction temperature is provided for the reaction zone；
The cleaning solution controlled in the reagent area flows into the reaction zone, anti-for cleaning remaining enzyme label in the reaction zone
Body, the substrate controlled in the reagent area flows into the reaction zone, and constant reaction temperature is provided for the reaction zone；
The light intensity data in each reactor is obtained respectively, and each reactor is determined according to the light intensity data in each reactor
The content of interior determinand.
2. on piece laboratory testing method according to claim 1, which is characterized in that described to provide perseverance for the reaction zone
Fixed reaction temperature, comprising:
To the thermostat module of the on piece laboratory testing device, the thermostat module is described anti-in the mobile on piece laboratory
Area is answered to provide constant reaction temperature.
3. on piece laboratory testing method according to claim 1 or 2, which is characterized in that described to be mentioned for the reaction zone
For constant reaction temperature, comprising:
Constant reaction temperature is provided for the reaction zone, and drives the liquid in the reaction zone at least two reaction
It is circulated in device.
4. on piece laboratory testing method according to claim 3, which is characterized in that the entrance and exit of the reaction zone
It is connect respectively by runner with first pump housing, the thermostat includes the first pump head, the liquid in the driving reaction zone
Body circulates at least two reactor, comprising:
First pump housing described in first pump head drive is controlled, so that the liquid in the reaction zone is at least two reaction
It is circulated in device.
5. on piece laboratory testing method according to claim 1, which is characterized in that described to obtain each reactor respectively
Interior light intensity data, comprising:
The light sensation module in the on piece laboratory to the on piece laboratory testing device is moved, and controls the light sensation module point
The light sensation data in each reactor are not obtained.
6. on piece laboratory testing method according to claim 1, which is characterized in that the method also includes:
Before sample flows into the reaction zone, pre-treatment is carried out to sample.
7. on piece laboratory testing method according to claim 6, which is characterized in that the on piece laboratory further includes sample
This entrance and the preceding processing area being arranged between the sample entrance port and the reaction zone, the pre-treatment area include the second pump
Body, the on piece laboratory testing device includes pre-processing module, and the pre-processing module includes plugging device and the second pump
Head, it is described that pre-treatment is carried out to sample, comprising:
To the pre-processing module, the capillary that the plugging device blocks the on piece laboratory is adopted in the mobile on piece laboratory
Vascular entrance；
Second pump housing described in second pump head drive is controlled to supply to the capillary heparin tube in the on piece laboratory.
8. on piece laboratory testing method according to claim 7, which is characterized in that the pre-processing module further includes
One liquid sensor, when the on piece laboratory is moved at the pre-processing module position, first liquid sensor
Positioned at the exit position of the reaction zone, the method also includes:
The reaction zone whether sample is full of the on piece laboratory is detected by first liquid sensor.
9. on piece laboratory testing method according to any one of claims 1 to 8, which is characterized in that the on piece experiment
Room detection device includes traction device, the movement on piece laboratory, comprising:
It controls the traction device and draws the on piece laboratory movement.
10. a kind of on piece laboratory testing system, which is characterized in that the on piece laboratory testing system includes on piece laboratory
And on piece laboratory testing device, the on piece laboratory include reagent area and reaction zone, the reaction zone includes successively
At least two reactors of connection, different reactor inner surfaces adhere to different reactants, the on piece laboratory testing dress
It sets including memory, at least one processor and is stored on the memory and can be executed at least one described processor
At least one program realizes the claims 1~9 when at least one described program is executed by least one described processor
Step in method described in one.
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Cited By (1)
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| CN110346584A (en) * | 2019-07-10 | 2019-10-18 | 深圳金迈隆电子技术有限公司 | A kind of on piece laboratory photoelectric detection system and method |
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