CN109765391B - Multi-index detection centrifugal test strip chip - Google Patents
Multi-index detection centrifugal test strip chip Download PDFInfo
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- CN109765391B CN109765391B CN201910195145.4A CN201910195145A CN109765391B CN 109765391 B CN109765391 B CN 109765391B CN 201910195145 A CN201910195145 A CN 201910195145A CN 109765391 B CN109765391 B CN 109765391B
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Abstract
The invention discloses a multi-index detection centrifugal test strip chip, which comprises: the cover plate layer, the first channel layer, the second channel layer and the bottom plate layer are sequentially arranged from top to bottom; wherein, the cover plate layer, the first channel layer, the second channel layer and the bottom plate layer are in sealing fit. The invention provides a multi-index detection centrifugal microfluidic chip which is clean, sanitary, accurate in detection and simple and convenient to operate; the simultaneous detection of multiple indexes is satisfied, and most of liquid after reaction is in the chip and is more sanitary than an independent test strip; the liquid enters the next stage through centrifugation, different liquids are guaranteed to enter at different times through different centrifugal rotating speeds, after the liquid to be detected and the cleaning liquid are added, the user is delivered to the centrifugal detection instrument to finish subsequent work, waiting time is not needed, subsequent manual operation is performed, and the degree of automation is improved.
Description
Technical Field
The invention relates to the technical field of microfluidic chip detection, in particular to a multi-index detection centrifugal test strip chip.
Background
Microfluidic chips are an emerging technology for manipulating and detecting chemical or biological samples on a chip. The microfluidic chip can integrate operation units such as sample preparation, reaction, separation, detection and the like into one chip with a few square centimeters, and the analysis process is automatically completed through the manipulation and control of fluid in a micro-channel network. The microfluidic chip technology has the characteristics of small sample injection amount, high integration level, easy realization of automatic control and high-throughput analysis, so that the biochemical reaction operation on the microfluidic chip is more convenient, rapid and low in cost compared with the conventional pretreatment of analysis samples.
The chromatographic test strip is widely applied to the fields of clinical diagnosis, food safety, environmental detection and the like. The detection method comprises chromaticity, fluorescence, magnetic detection and the like, and depends on the marker in the binding pad, and if quantitative detection such as fluorescence is carried out, cleaning liquid is required to be dripped after the reaction of the liquid to be detected is finished, unbound marker in the detection area is removed, and interference is reduced. In the conventional operation, an operator needs to manually add cleaning liquid after adding the liquid to be detected for a period of time for reaction, and then visual or simple detection devices are used for reading results, so that the degree of automation is poor, and the detection efficiency is affected.
Therefore, how to provide a centrifugal microfluidic test strip chip for multi-index detection, which is clean, sanitary, accurate in detection and simple and convenient to operate, is a problem to be solved by those skilled in the art.
Disclosure of Invention
In view of the above, the invention provides a multi-index detection centrifugal test strip chip, which combines a microfluidic chip with a chromatographic test strip to meet the requirement of multi-index simultaneous detection, and most of liquid after reaction is in the chip and is more sanitary than an independent test strip; the liquid enters the next stage through centrifugation, different liquids are guaranteed to enter at different times through different centrifugal rotating speeds, after the liquid to be detected and the cleaning liquid are added, the user is delivered to the centrifugal detection instrument to finish subsequent work, waiting time is not needed, subsequent manual operation is performed, and the degree of automation is improved.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
a multi-index test centrifugal test strip chip comprising: the cover plate layer, the first channel layer, the second channel layer and the bottom plate layer are sequentially arranged from top to bottom; wherein the cover plate layer, the first channel layer, the second channel layer and the bottom plate layer are in sealing fit;
the cover plate layer is provided with a visual window, a cover plate layer ventilation port, a cover plate layer liquid sample injection hole to be measured, a cover plate layer cleaning liquid sample injection hole, a cover plate layer liquid vent hole to be measured, a cover plate layer cleaning liquid vent hole, a cover plate layer sample injection pool vent hole and a cover plate chip fixing hole;
the first channel layer comprises a detection area through hole I, a detection area through hole II, a first channel layer liquid separating pool, a first channel layer cleaning liquid separating pool, a first channel layer liquid inlet channel, a first channel layer cleaning liquid inlet channel, a first channel layer sample injection pool, a sample injection pool ventilation channel, a first channel layer liquid sample injection hole to be detected, a first channel layer cleaning liquid sample injection hole, a first channel layer liquid vent hole to be detected, a first channel layer cleaning liquid vent hole, a first channel layer sample injection pool vent hole and a first channel layer chip fixing hole;
the detection area through hole I corresponds to and is communicated with the cover plate layer ventilation opening; the detection area through hole II is arranged corresponding to the visual window;
the first channel layer liquid inlet channel of the liquid to be detected is communicated with the first channel layer liquid separating pool and the first channel layer sample injection pool; the first channel layer cleaning liquid inlet channel is communicated with the first channel layer cleaning liquid separating pool and the first channel layer sample injection pool; the first channel layer sample injection pool vent hole is arranged corresponding to the cover plate layer sample injection pool vent hole, and the sample injection pool vent channel is communicated with the first channel layer sample injection pool vent hole and the first channel layer sample injection pool;
the second channel layer comprises a detection area through hole III, a detection area through hole IV, a second channel layer liquid separating pool to be detected, a second channel layer cleaning liquid separating pool, a second channel layer liquid main channel to be detected, a second channel layer cleaning liquid main channel, a second channel layer sample injection pool, a second channel layer liquid sample injection hole, a second channel layer cleaning liquid sample injection hole, a second channel layer liquid to be detected vent hole, a second channel layer cleaning liquid vent hole and a second channel layer chip fixing hole;
the detection area through hole III is correspondingly arranged with the detection area through hole I; the detection area through hole IV is correspondingly arranged with the detection area through hole II;
the second channel layer to-be-detected liquid sample injection hole is correspondingly arranged with the first channel layer to-be-detected liquid sample injection hole and the cover plate layer to-be-detected liquid sample injection hole, the second channel layer to-be-detected liquid vent hole is correspondingly arranged with the first channel layer to-be-detected liquid vent hole and the cover plate layer to-be-detected liquid vent hole, the second channel layer to-be-detected liquid main channel is communicated with the second channel layer to-be-detected liquid sample injection hole, the second channel layer to-be-detected liquid sub-liquid pool and the second channel layer to-be-detected liquid vent hole, and the second channel layer to-be-detected liquid sub-liquid pool is correspondingly communicated with the first channel layer to-be-detected liquid sub-liquid pool;
the second channel layer cleaning solution sample injection hole is correspondingly arranged with the first channel layer cleaning solution sample injection hole and the cover plate layer cleaning solution sample injection hole, the second channel layer cleaning solution vent hole is correspondingly arranged with the first channel layer cleaning solution vent hole and the cover plate layer cleaning solution vent hole, the second channel layer cleaning solution main channel is communicated with the second channel layer cleaning solution sample injection hole, the second channel layer cleaning solution sub-liquid pool and the second channel layer cleaning solution vent hole, and the second channel layer cleaning solution sub-liquid pool is correspondingly communicated with the first channel layer cleaning solution sub-liquid pool; the second channel layer sample injection pool is correspondingly arranged and communicated with the first channel layer sample injection pool;
the bottom plate layer is provided with a chromatographic test strip and a bottom plate chip fixing hole, wherein the chromatographic test strip consists of a sample pad, a nitrocellulose membrane, a binding pad and an absorption pad, the nitrocellulose membrane is positioned in the middle, and the sample pad and the absorption pad are respectively positioned at two ends; the conjugate pad is located between the sample pad and the nitrocellulose membrane;
the chromatographic test strips are radially provided with a plurality of chromatographic test strips, and each chromatographic test strip is respectively provided with a corresponding second channel layer sample injection pool, a corresponding first channel layer to-be-detected liquid inlet channel, a corresponding first channel layer to-be-detected liquid separating pool, a corresponding second channel layer to-be-detected liquid separating pool, a corresponding first channel layer cleaning liquid inlet channel, a corresponding first channel layer cleaning liquid separating pool, a corresponding second channel layer cleaning liquid separating pool, a corresponding sample injection pool ventilation channel, a corresponding first channel layer sample injection pool ventilation hole, a corresponding cover plate layer sample injection pool ventilation hole, a corresponding detection area through hole I, a corresponding detection area through hole II, a corresponding detection area through hole III and a corresponding detection area through hole IV in a matching manner;
the sample pad of the chromatographic test strip is arranged corresponding to the second channel layer sample injection pool, the second channel layer sample injection pool is communicated with the bottom plate layer, and liquid in the second channel layer sample injection pool can be contacted with the sample pad, so that the liquid in the second channel layer sample injection pool can flow into the sample pad, and gradually enter the binding pad, the nitrocellulose membrane and the absorption pad through the chromatographic action to perform a series of reactions; the nitrocellulose membrane of the chromatographic test strip is correspondingly arranged with the detection area through hole IV, the detection area through hole II and the visual window, and the reaction state of the chromatographic test strip can be observed through the visual window, the detection area through hole IV and the detection area through hole II; the absorption pad is correspondingly arranged with the detection area through hole III, the detection area through hole I and the cover plate layer ventilation opening.
Preferably, in the multi-index detection centrifugal test strip chip, the visual window is an open through hole.
Preferably, in the multi-index detection centrifugal test strip chip, the shape of the cleaning solution inlet channel of the first channel layer includes, but is not limited to, a wave shape, and the wave shape channel can increase the channel length and can also be a zigzag shape or a zigzag shape.
Preferably, in the multi-index detection centrifugal test strip chip, the cover plate chip fixing hole, the first channel layer chip fixing hole, the second channel layer chip fixing hole and the bottom plate chip fixing hole are identical in shape and overlap, so that a chip fixing hole penetrating through the multi-index detection centrifugal test strip chip is formed. In the centrifugation process, the multi-index detection centrifugal test strip chip is fixed on the centrifugal detector through the chip fixing hole, and the multi-index detection centrifugal test strip chip rotates to be centrifuged by taking the chip fixing hole as a circle center.
Preferably, in the multi-index detection centrifugal test strip chip, the first channel layer cleaning solution separating pool, the second channel layer cleaning solution separating pool, the first channel layer liquid separating pool to be detected and the second channel layer liquid separating pool to be detected are all located at one side, close to the chip fixing hole, of the second channel layer sample feeding pool. In the centrifugation process, the liquid in the first channel layer cleaning liquid separating pool, the second channel layer cleaning liquid separating pool, the first channel layer liquid separating pool to be detected and the second channel layer liquid separating pool flows into the second channel layer sample injection pool under the action of centrifugal force.
Preferably, in the multi-index detection centrifugal test strip chip, the second channel layer cleaning solution main channel, the second channel layer cleaning solution liquid separating pool, the second channel layer main channel to be tested, the second channel layer liquid separating pool and the second channel layer sample feeding pool are sequentially arranged at one side of the chip fixing hole from near to far.
Preferably, in the above multi-index detection centrifugal test strip chip, the driving force of the liquid in the multi-index detection centrifugal test strip chip is centrifugal force.
Preferably, in the multi-index detection centrifugal test strip chip, the second channel layer liquid separating pool, the second channel layer cleaning liquid separating pool, the second channel layer liquid main channel and the second channel layer cleaning liquid main channel are isolated from the bottom plate layer, and liquid in the second channel layer liquid separating pool and the second channel layer cleaning liquid main channel cannot flow into the bottom plate layer.
Preferably, in the multi-index detection centrifugal test strip chip, the main channel of the liquid to be detected in the second channel layer and the main channel of the cleaning liquid in the second channel layer are pipelines arranged in the second channel layer; the first channel layer to-be-measured liquid inlet channel and the first channel layer cleaning liquid inlet channel are pipelines arranged in the first channel layer.
Preferably, in the multi-index detection centrifugal microfluidic test paper chip, a marker is disposed in the bonding pad, so that the liquid to be detected reacts with the marker.
When the liquid is added, the liquid to be detected is added from a liquid sample injection hole of a cover plate layer, and the liquid sample injection hole of a first channel layer, the liquid sample injection hole of a second channel layer and a main channel of the liquid to be detected are filled with a liquid separating pool of the liquid to be detected of the first channel layer and a liquid separating pool of the liquid to be detected of the second channel layer one by one, and exhaust is carried out through a liquid vent hole of the liquid to be detected of the second channel layer, a liquid vent hole of the liquid to be detected of the first channel layer and a liquid vent hole of the liquid to be detected of the cover plate layer; adding cleaning solution from a cover plate layer cleaning solution sample injection hole, filling a first channel layer cleaning solution sub-liquid pool and a second channel layer cleaning solution sub-liquid pool one by one through a first channel layer cleaning solution sample injection hole, a second channel layer cleaning solution sample injection hole and a second channel layer cleaning solution main channel, and exhausting through a second channel layer cleaning solution vent hole, a first channel layer cleaning solution vent hole and a cover plate layer cleaning solution vent hole;
compared with the prior art, the invention discloses a multi-index detection centrifugal microfluidic test paper chip, which combines the microfluidic chip with a chromatographic test strip, so that the simultaneous detection of multiple indexes is satisfied, the working efficiency is improved, and most of liquid after reaction is in the chip and is more sanitary than the independent test strip; the liquid enters the next stage through centrifugation, different liquids are guaranteed to enter at different times through different centrifugal rotating speeds, after the liquid to be detected and the cleaning liquid are added, the user is delivered to the centrifugal detection instrument to finish subsequent work, waiting time is not needed, subsequent manual operation is performed, and the degree of automation is improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required to be used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are only embodiments of the present invention, and that other drawings can be obtained according to the provided drawings without inventive effort for a person skilled in the art.
FIG. 1 is a three-dimensional exploded view of the present invention;
FIG. 2 is a diagram of a cover plate layer structure;
FIG. 3 is a diagram of a first channel layer structure;
FIG. 4 is a diagram of a second channel layer structure;
FIG. 5 is a schematic view of a floor layer structure;
FIG. 6 is a schematic diagram of a chromatographic test strip.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The embodiment of the invention discloses a multi-index detection centrifugal test strip chip, which combines a microfluidic chip with a chromatographic test strip, meets the requirements of multi-index simultaneous detection, improves the working efficiency, and ensures that most of liquid after reaction is in the chip and is more sanitary than an independent test strip; the liquid enters the next stage through centrifugation, different liquids are guaranteed to enter at different times through different centrifugal rotating speeds, after the liquid to be detected and the cleaning liquid are added, the user is delivered to the centrifugal detection instrument to finish subsequent work, waiting time is not needed, subsequent manual operation is performed, and the degree of automation is improved.
A multi-index test centrifugal test strip chip comprising: the cover plate layer 1, the first channel layer 2, the second channel layer 3 and the bottom plate layer 4 are sequentially arranged from top to bottom; wherein, the cover plate layer 1, the first channel layer 2, the second channel layer 3 and the bottom plate layer 4 are in sealing fit;
the cover plate layer 1 is provided with a visual window 11, a cover plate layer ventilation port 12, a cover plate layer liquid sample injection hole 13 to be detected, a cover plate layer cleaning liquid sample injection hole 16, a cover plate layer liquid vent hole 15 to be detected, a cover plate layer cleaning liquid vent hole 14, a cover plate layer sample injection pool vent hole 17 and a cover plate chip fixing hole 18;
the first channel layer 2 comprises a detection area through hole I21, a detection area through hole II 22, a first channel layer liquid separating pool 23, a first channel layer cleaning liquid separating pool 24, a first channel layer liquid inlet channel 25, a first channel layer cleaning liquid inlet channel 26, a first channel layer sample injection pool 27, a sample injection pool ventilation channel 28, a first channel layer liquid sample injection hole 29 to be detected, a first channel layer cleaning liquid sample injection hole 212, a first channel layer liquid sample vent hole 211, a first channel layer cleaning liquid vent hole 210, a first channel layer sample injection pool vent hole 213 and a first channel layer chip fixing hole 214;
the detection area through hole I21 corresponds to and is communicated with the cover plate layer ventilation port 12; the detection area through hole II 22 is arranged corresponding to the visual window 11;
the first channel layer liquid inlet channel 25 is communicated with the first channel layer liquid separating pool 23 and the first channel layer sample feeding pool 27; the first channel layer cleaning liquid inlet channel 26 is communicated with the first channel layer cleaning liquid separating pool 24 and the first channel layer sample introduction pool 27; the first channel layer sample introduction tank vent hole 213 is arranged corresponding to the cover plate layer sample introduction tank vent hole 17, and the sample introduction tank vent channel 28 is communicated with the first channel layer sample introduction tank vent hole 213 and the first channel layer sample introduction tank 27;
the second channel layer 3 includes a detection area through hole iii 31, a detection area through hole iv 32, a second channel layer to-be-detected liquid separating pool 33, a second channel layer cleaning liquid separating pool 312, a second channel layer to-be-detected liquid main channel 34, a second channel layer cleaning liquid main channel 35, a second channel layer sample introduction pool 36, a second channel layer to-be-detected liquid sample introduction hole 37, a second channel layer cleaning liquid sample introduction hole 310, a second channel layer to-be-detected liquid vent hole 39, a second channel layer cleaning liquid vent hole 38, and a second channel layer chip fixing hole 311;
the detection area through hole III 31 is correspondingly arranged with the detection area through hole I21; the detection area through hole IV 32 is correspondingly arranged with the detection area through hole II 22;
the second channel layer to-be-measured liquid sample injection hole 37 is correspondingly arranged with the first channel layer to-be-measured liquid sample injection hole 29 and the cover plate layer to-be-measured liquid sample injection hole 13, the second channel layer to-be-measured liquid vent hole 39 is correspondingly arranged with the first channel layer to-be-measured liquid vent hole 211 and the cover plate layer to-be-measured liquid vent hole 15, the second channel layer to-be-measured liquid main channel 34 is communicated with the second channel layer to-be-measured liquid sample injection hole 37, the second channel layer to-be-measured liquid sub-liquid pool 33 and the second channel layer to-be-measured liquid vent hole 39, and the second channel layer to-be-measured liquid sub-liquid pool 33 is correspondingly communicated with the first channel layer to-be-measured liquid sub-liquid pool 23;
the second channel layer cleaning liquid sample injection hole 310 is correspondingly arranged with the first channel layer cleaning liquid sample injection hole 212 and the cover plate layer cleaning liquid sample injection hole 16, the second channel layer cleaning liquid vent hole 38 is correspondingly arranged with the first channel layer cleaning liquid vent hole 210 and the cover plate layer cleaning liquid vent hole 14, the second channel layer cleaning liquid main channel 35 is communicated with the second channel layer cleaning liquid sample injection hole 310, the second channel layer cleaning liquid sub-liquid pool 312 and the second channel layer cleaning liquid vent hole 38, and the second channel layer cleaning liquid sub-liquid pool 312 is correspondingly communicated with the first channel layer cleaning liquid sub-liquid pool 24; the second channel layer sample injection pool 36 is correspondingly arranged and communicated with the first channel layer sample injection pool 27;
the bottom plate layer 4 is provided with a chromatography test strip 41 and a bottom plate chip fixing hole 42, wherein the chromatography test strip 41 consists of a sample pad 43, a nitrocellulose membrane 44, a binding pad 45 and an absorption pad 46, the nitrocellulose membrane 44 is positioned in the middle, and the sample pad 43 and the absorption pad 46 are respectively positioned at two ends; a conjugate pad 45 is located between the sample pad 43 and the nitrocellulose membrane 44;
the chromatographic test strips 41 are radially provided with a plurality of chromatographic test strips 41, and each chromatographic test strip 41 is respectively provided with a corresponding second channel layer sample injection pool 36, a first channel layer sample injection pool 27, a first channel layer liquid inlet channel 25 to be tested, a first channel layer liquid separating pool 23 to be tested, a second channel layer liquid separating pool 33 to be tested, a first channel layer cleaning liquid inlet channel 26, a first channel layer cleaning liquid separating pool 24, a second channel layer cleaning liquid separating pool 312, a sample injection pool ventilation channel 28, a first channel layer sample injection pool ventilation hole 213, a cover plate layer sample injection pool ventilation hole 17, a detection area through hole I21, a detection area through hole II 22, a detection area through hole III 31 and a detection area through hole IV 32 in a matching manner;
the sample pad 43 of the chromatographic test strip 41 is arranged corresponding to the second channel layer sample introduction pool 36, the second channel layer sample introduction pool 36 is communicated with the bottom plate layer 4, and the liquid in the second channel layer sample introduction pool 36 can be contacted with the sample pad 43, so that the liquid in the second channel layer sample introduction pool 36 can flow into the sample pad 43, and gradually enter the combination pad 45, the nitrocellulose membrane 44 and the absorption pad 46 by the chromatographic action to perform a series of reactions; the nitrocellulose membrane 44 of the chromatographic test strip 4 is correspondingly arranged with the detection zone through hole IV 32, the detection zone through hole II 22 and the visual window 11, and the reaction state of the chromatographic test strip 41 can be observed through the visual window 11, the detection zone through hole IV 32 and the detection zone through hole II 22; the absorption pad 46 is disposed corresponding to the detection area through hole III 31, the detection area through hole I21, and the cover layer ventilation opening 12.
In order to further optimize the above technical solution, the viewing window 11 is an open through hole.
To further optimize the above technical solution, the shape of the cleaning liquid inlet channel 26 of the first channel layer includes, but is not limited to, a wave shape, which can increase the channel length, and may be a zigzag shape or a zigzag shape.
In order to further optimize the above technical solution, the cover plate chip fixing hole 18, the first channel layer chip fixing hole 214, the second channel layer chip fixing hole 311, and the bottom plate chip fixing hole 42 are identical in shape and overlap, so as to form a chip fixing hole penetrating through a multi-index detection centrifugal test strip chip. In the centrifugation process, a multi-index detection centrifugal test strip chip is fixed on a centrifugal detector through a chip fixing hole, and the multi-index detection centrifugal test strip chip rotates to perform centrifugation by taking the chip fixing hole as a circle center.
To further optimize the above technical solution, the first channel layer cleaning solution separating tank 24, the second channel layer cleaning solution separating tank 312, the first channel layer to-be-measured solution separating tank 23, and the second channel layer to-be-measured solution separating tank 33 are all located at one side of the second channel layer sample feeding tank 36 near the chip fixing hole. In the centrifugation process, the liquid in the first channel layer cleaning liquid separating tank 24, the second channel layer cleaning liquid separating tank 312, the first channel layer liquid separating tank 23 and the second channel layer liquid separating tank 33 flows into the second channel layer sample feeding tank 36 under the action of centrifugal force.
In order to further optimize the above technical solution, the second channel layer cleaning solution main channel 35, the second channel layer cleaning solution sub-tank 312, the second channel layer main channel 34, the second channel layer liquid sub-tank 33, and the second channel layer sample injection tank 36 are sequentially arranged from near to far at one side of the chip fixing hole.
In order to further optimize the technical scheme, the driving force of the liquid in the multi-index detection centrifugal test strip chip is centrifugal force.
In order to further optimize the above technical solution, the second channel layer to-be-measured liquid separating tank 33, the second channel layer cleaning liquid separating tank 312, the second channel layer to-be-measured liquid main channel 34, and the second channel layer cleaning liquid main channel 35 are isolated from the bottom plate layer 4, and the liquid therein does not flow into the bottom plate layer 4.
In order to further optimize the technical scheme, the main channel 34 of the liquid to be detected of the second channel layer and the main channel 35 of the cleaning liquid of the second channel layer are pipelines which are internally arranged in the second channel layer 3; the first channel layer liquid inlet channel 25 to be measured and the first channel layer cleaning liquid inlet channel 26 are pipelines built in the first channel layer 2.
To further optimize the above technical solution, the conjugate pad 45 is provided with a label, so that the liquid to be measured reacts with the label.
During liquid adding, the liquid to be detected is added from the cover plate layer liquid sample injection hole 13, and the first channel layer liquid sample separating pool 23 and the second channel layer liquid sample separating pool 33 are filled one by one through the first channel layer liquid sample injection hole 29, the second channel layer liquid sample injection hole 37 and the second channel layer liquid sample main channel 34 to be detected, and exhaust is carried out through the second channel layer liquid sample vent 39, the first channel layer liquid sample vent 211 and the cover plate layer liquid sample vent 15; adding cleaning solution from the cover plate layer cleaning solution injection hole 16, filling the first channel layer cleaning solution sub-tank 24 and the second channel layer cleaning solution sub-tank 312 one by one through the first channel layer cleaning solution injection hole 212, the second channel layer cleaning solution injection hole 310 and the second channel layer cleaning solution main channel 35, and exhausting through the second channel layer cleaning solution vent hole 38, the first channel layer cleaning solution vent hole 210 and the cover plate layer cleaning solution vent hole 14;
the multi-index detection centrifugal test strip chip is fixed on a rotating shaft of a centrifugal detector through a chip fixing hole, so that the multi-index detection centrifugal test strip chip rotates to be centrifuged by taking the chip fixing hole as a circle center, and the liquid flowing process is as follows:
(1) Centrifuging at a low rotation speed, wherein the liquid to be detected enters a first channel layer sample introduction pond 27 and a second channel layer sample introduction pond 36 from a first channel layer liquid to be detected separating pond 23 and a second channel layer liquid to be detected separating pond 33 through a first channel layer liquid to be detected inlet channel 25;
(2) After the centrifugal detector acts at a low speed, the liquid to be detected in the first channel layer sample injection pool 27 and the second channel layer sample injection pool 36 is in contact with the sample pad 43 of the chromatographic test strip 41, gradually enters the combination pad 45, the nitrocellulose membrane 44 and the absorption pad 46 through the chromatographic action, reacts with the marker in the combination pad 45, and discharges the gas in the space where the chromatographic test strip 41 is positioned through the cover plate layer ventilation port 12, the detection area through hole I21 and the detection area through hole III 31, and balances the air pressure;
(3) High-speed centrifugation, wherein the cleaning fluid enters the first channel layer sample introduction tank 27 and the second channel layer sample introduction tank 36 from the first channel layer cleaning fluid sub-tank 24 and the second channel layer cleaning fluid sub-tank 312 through the first channel layer cleaning fluid inlet channel 26;
(4) After high-speed action of the centrifugal detector, the sample pad 43 of the chromatographic test strip 41 contacted with cleaning liquid in the first channel layer sample injection pool 27 and the second channel layer sample injection pool 36 enters the binding pad 45, the nitrocellulose membrane 44 and the absorption pad 46 through chromatographic action, residual unbound reagent on the nitrocellulose membrane 44 is washed out, interference during result reading is avoided, and gas in the space where the chromatographic test strip 41 is located is discharged through the cover plate layer ventilation port 12, the detection area through hole I21 and the detection area through hole III 31 to balance air pressure.
(5) The reaction results on the nitrocellulose membrane 44 were observed through the detection zone through hole II 22, the detection zone through hole IV 32, and the viewing window 11.
In the present specification, each embodiment is described in a progressive manner, and each embodiment is mainly described in a different point from other embodiments, and identical and similar parts between the embodiments are all enough to refer to each other. For the device disclosed in the embodiment, since it corresponds to the method disclosed in the embodiment, the description is relatively simple, and the relevant points refer to the description of the method section.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (9)
1. A multi-index test centrifugal test strip chip, comprising: the cover plate layer, the first channel layer, the second channel layer and the bottom plate layer are sequentially arranged from top to bottom; wherein the cover plate layer, the first channel layer, the second channel layer and the bottom plate layer are in sealing fit;
the cover plate layer is provided with a visual window, a cover plate layer ventilation port, a cover plate layer liquid sample injection hole to be measured, a cover plate layer cleaning liquid sample injection hole, a cover plate layer liquid vent hole to be measured, a cover plate layer cleaning liquid vent hole, a cover plate layer sample injection pool vent hole and a cover plate chip fixing hole;
the first channel layer comprises a detection area through hole I, a detection area through hole II, a first channel layer liquid separating pool, a first channel layer cleaning liquid separating pool, a first channel layer liquid inlet channel, a first channel layer cleaning liquid inlet channel, a first channel layer sample injection pool, a sample injection pool ventilation channel, a first channel layer liquid sample injection hole to be detected, a first channel layer cleaning liquid sample injection hole, a first channel layer liquid vent hole to be detected, a first channel layer cleaning liquid vent hole, a first channel layer sample injection pool vent hole and a first channel layer chip fixing hole;
the detection area through hole I corresponds to and is communicated with the cover plate layer ventilation opening; the detection area through hole II is arranged corresponding to the visual window;
the first channel layer liquid inlet channel of the liquid to be detected is communicated with the first channel layer liquid separating pool and the first channel layer sample injection pool; the first channel layer cleaning liquid inlet channel is communicated with the first channel layer cleaning liquid separating pool and the first channel layer sample injection pool; the first channel layer sample injection pool vent hole is arranged corresponding to the cover plate layer sample injection pool vent hole, and the sample injection pool vent channel is communicated with the first channel layer sample injection pool vent hole and the first channel layer sample injection pool;
the second channel layer comprises a detection area through hole III, a detection area through hole IV, a second channel layer liquid separating pool to be detected, a second channel layer cleaning liquid separating pool, a second channel layer liquid main channel to be detected, a second channel layer cleaning liquid main channel, a second channel layer sample injection pool, a second channel layer liquid sample injection hole, a second channel layer cleaning liquid sample injection hole, a second channel layer liquid to be detected vent hole, a second channel layer cleaning liquid vent hole and a second channel layer chip fixing hole;
the detection area through hole III is correspondingly arranged with the detection area through hole I; the detection area through hole IV is correspondingly arranged with the detection area through hole II;
the second channel layer to-be-detected liquid sample injection hole is correspondingly arranged with the first channel layer to-be-detected liquid sample injection hole and the cover plate layer to-be-detected liquid sample injection hole, the second channel layer to-be-detected liquid vent hole is correspondingly arranged with the first channel layer to-be-detected liquid vent hole and the cover plate layer to-be-detected liquid vent hole, the second channel layer to-be-detected liquid main channel is communicated with the second channel layer to-be-detected liquid sample injection hole, the second channel layer to-be-detected liquid sub-liquid pool and the second channel layer to-be-detected liquid vent hole, and the second channel layer to-be-detected liquid sub-liquid pool is correspondingly communicated with the first channel layer to-be-detected liquid sub-liquid pool;
the second channel layer cleaning solution sample injection hole is correspondingly arranged with the first channel layer cleaning solution sample injection hole and the cover plate layer cleaning solution sample injection hole, the second channel layer cleaning solution vent hole is correspondingly arranged with the first channel layer cleaning solution vent hole and the cover plate layer cleaning solution vent hole, the second channel layer cleaning solution main channel is communicated with the second channel layer cleaning solution sample injection hole, the second channel layer cleaning solution sub-liquid pool and the second channel layer cleaning solution vent hole, and the second channel layer cleaning solution sub-liquid pool is correspondingly communicated with the first channel layer cleaning solution sub-liquid pool; the second channel layer sample injection pool is correspondingly arranged and communicated with the first channel layer sample injection pool;
the bottom plate layer is provided with a chromatographic test strip and a bottom plate chip fixing hole, wherein the chromatographic test strip consists of a sample pad, a nitrocellulose membrane, a binding pad and an absorption pad, the nitrocellulose membrane is positioned in the middle, and the sample pad and the absorption pad are respectively positioned at two ends; the conjugate pad is located between the sample pad and the nitrocellulose membrane;
the chromatographic test strips are radially provided with a plurality of chromatographic test strips, and each chromatographic test strip is respectively provided with a corresponding second channel layer sample injection pool, a corresponding first channel layer to-be-detected liquid inlet channel, a corresponding first channel layer to-be-detected liquid separating pool, a corresponding second channel layer to-be-detected liquid separating pool, a corresponding first channel layer cleaning liquid inlet channel, a corresponding first channel layer cleaning liquid separating pool, a corresponding second channel layer cleaning liquid separating pool, a corresponding sample injection pool ventilation channel, a corresponding first channel layer sample injection pool ventilation hole, a corresponding cover plate layer sample injection pool ventilation hole, a corresponding detection area through hole I, a corresponding detection area through hole II, a corresponding detection area through hole III and a corresponding detection area through hole IV in a matching manner;
the sample pad of the chromatographic test strip is arranged corresponding to the second channel layer sample injection pool, the second channel layer sample injection pool is communicated with the bottom plate layer, and liquid in the second channel layer sample injection pool can be in contact with the sample pad; the nitrocellulose membrane of the chromatographic test strip is correspondingly arranged with the detection area through hole IV, the detection area through hole II and the visible window; the absorption pad is correspondingly arranged with the detection area through hole III, the detection area through hole I and the cover plate layer ventilation opening;
the detection area through hole III, the second channel layer cleaning solution main channel, the second channel layer cleaning solution liquid separating pool, the second channel layer main channel to be detected, the second channel layer liquid separating pool and the second channel layer sample feeding pool are sequentially distributed on one side of the chip fixing hole from near to far.
2. The multi-index test centrifugal test strip chip according to claim 1, wherein the visual window is an open through hole.
3. The multi-index test centrifugal test strip chip according to claim 1, wherein the shape of the first channel layer cleaning liquid inlet channel comprises, but is not limited to, a wave shape.
4. The multi-index test centrifugal test strip chip according to claim 1, wherein the cover plate chip fixing hole is identical to and overlapped with the first channel layer chip fixing hole, the second channel layer chip fixing hole and the bottom plate chip fixing hole in shape, so as to form a chip fixing hole penetrating through the multi-index test centrifugal test strip chip.
5. The multi-index detection centrifugal test strip chip according to claim 4, wherein the first channel layer cleaning solution separating tank, the second channel layer cleaning solution separating tank, the first channel layer to-be-detected solution separating tank and the second channel layer to-be-detected solution separating tank are all located at one side, close to the chip fixing hole, of the second channel layer sample feeding tank.
6. The multi-index test centrifugal test strip chip according to claim 1, wherein the driving force of the liquid in the multi-index test centrifugal test strip chip is centrifugal force.
7. The multi-index test centrifugal test strip chip according to claim 1, wherein the second channel layer to-be-tested liquid sub-tank, the second channel layer to-be-tested liquid main channel, and the second channel layer to-be-tested liquid main channel are isolated from the bottom plate layer.
8. The multi-index detection centrifugal test strip chip according to claim 1, wherein the main channel of the liquid to be detected in the second channel layer and the main channel of the cleaning liquid in the second channel layer are pipelines arranged in the second channel layer; the first channel layer to-be-measured liquid inlet channel and the first channel layer cleaning liquid inlet channel are pipelines arranged in the first channel layer.
9. The multi-index test centrifugal test strip chip according to claim 1, wherein a marker is provided in the binding pad.
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| CN201910195145.4A CN109765391B (en) | 2019-03-14 | 2019-03-14 | Multi-index detection centrifugal test strip chip |
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| CN110568199B (en) * | 2019-09-12 | 2022-05-31 | 重庆科技学院 | Multi-channel fluorescence immunochromatography detection microfluidic chip |
| CN110568203B (en) * | 2019-09-12 | 2022-05-24 | 重庆科技学院 | Application method of multi-channel fluorescence immunochromatographic microfluidic chip |
| DE102022106693B3 (en) | 2022-03-22 | 2023-03-16 | Dermagnostix GmbH | Centrifugal Microfluidic Biochip |
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| CN104502617A (en) * | 2014-12-24 | 2015-04-08 | 杭州霆科生物科技有限公司 | Full-automatic and high-throughput micro-fluidic chip system and method for detecting pesticide residue |
| CN106513063B (en) * | 2016-06-06 | 2018-09-07 | 苏州汶颢芯片科技有限公司 | The centrifugal chip and its mixed method of consecutive reaction can be achieved |
| CN206229378U (en) * | 2016-08-24 | 2017-06-09 | 杭州霆科生物科技有限公司 | A kind of centrifugal routine urinalysis detection micro-fluidic chip |
| CN109212201A (en) * | 2017-07-03 | 2019-01-15 | 杭州霆科生物科技有限公司 | A kind of centrifugal type microfludic chip for hepatitis B five detections in serum |
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| CN110568199B (en) * | 2019-09-12 | 2022-05-31 | 重庆科技学院 | Multi-channel fluorescence immunochromatography detection microfluidic chip |
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