WO2021077591A1

WO2021077591A1 - Microfluidic chip and in vitro test system

Info

Publication number: WO2021077591A1
Application number: PCT/CN2019/126900
Authority: WO
Inventors: 白孟斌; 万惠芳; 冷杰
Original assignee: 广州万孚生物技术股份有限公司
Priority date: 2019-10-21
Filing date: 2019-12-20
Publication date: 2021-04-29
Also published as: CN112756018A

Abstract

Disclosed are a microfluidic chip (10) and an in vitro test system comprising the microfluidic chip (10). After a sample solution has been added to a sample addition cavity (110) of a microfluidic chip (10), spin centrifugation is performed, such that the sample solution enters, and gradually fills, a dosing cavity (130) and a first waste liquid cavity (140) by means of a first microfluidic connecting channel (120). Excess sample solution overflows into a second waste liquid cavity (160) by means of a microfluidic overflow channel (150). In the invention, centrifugation is performed on a sample solution to separate solid impurities from a solution under test, such that the solid impurities are sedimented in the first waste liquid cavity (140) by centrifugation while the solution under test remains in the dosing cavity (130) at an end close to the center, thereby realizing separation and dosing of the sample solution.

Description

Microfluidic chip and in vitro detection system

This application claims the priority of a Chinese patent application filed with the Chinese Patent Office with application number 201911001358.5 on October 21, 2019. The entire content of this application is incorporated into this application by reference.

Technical field

This application relates to the field of in vitro diagnostic technology, such as a microfluidic chip and an in vitro detection system.

Background technique

In Vitro Diagnosis (IVD) belongs to the medical and biological industry, which refers to taking blood, body fluids, tissues and other samples from the human body, and using in vitro testing reagents, instruments, etc. to test and verify the samples in order to prevent diseases , Diagnosis, treatment testing, late-stage observation, health evaluation, genetic disease prediction, etc. In vitro diagnosis is divided into three categories: biochemical diagnosis, immunodiagnosis and molecular diagnosis according to methodology, as well as point-of-care testing (POCT) differentiated from biochemical, immunological and molecular diagnosis. Dry chemical reaction is a type of biochemical diagnosis, which uses biochemical reagents to react with a specific substrate, and then quantitatively detects the concentration of the target through an instrument to calculate certain biochemical indicators of the human body. Traditional biochemical diagnosis needs to be tested on a large-scale biochemical analyzer, which leads to high reagent consumption and insufficient flexibility. The general dry-type biochemical POCT diagnosis method has a low test throughput, and generally only one or a few tests can be tested at a time. Samples, one or several items. Microfluidics technology (Microfluidics) can integrate basic operation units such as sample preparation, reaction, separation, and detection in biological, chemical, and medical analysis processes on the chip, automatically completing the entire analysis process, greatly improving the detection efficiency, and at the same time It has the advantages of miniaturization and automation, so it is more and more widely used in the field of POCT.

In the field of biochemical testing, Abaxis of the United States took the lead in the development of microfluidic chips for biochemical testing. Domestic companies such as Tianjin Micronanochip and Chengdu Smart have similar microfluidic chip development. The process of quantification and distribution of whole blood samples with traditional products often requires separation of whole blood and serum quantification. Therefore, multiple centrifugation and quantification are required, which makes the sample processing time longer and leads to detection time. Too extended.

Summary of the invention

The embodiments of the present application provide a microfluidic chip capable of improving sample processing efficiency and an in vitro detection system containing the microfluidic chip.

A microfluidic chip has a separation and quantification unit, the separation and quantification unit includes a sample addition cavity, a first connecting microchannel, a quantification cavity, a first waste liquid cavity, an overflow microchannel, and a second waste The liquid cavity; the sample loading cavity has a sample hole; the quantitative cavity communicates with the sample cavity through the first connecting microchannel; the first waste liquid cavity and the quantitative The cavity is in communication; the second waste liquid cavity is in communication with the quantitative cavity through the overflow microchannel; the separation and quantification unit has a proximal end close to the center of rotation during centrifugation; the sample adding cavity Body is closer to the proximal end than the dosing cavity; the dosing cavity is closer to the proximal end than the first waste liquid cavity; when the dosing cavity is filled with liquid, excess liquid The second waste liquid cavity can enter the second waste liquid cavity through the overflow microchannel, and the overall distance of the second waste liquid cavity from the proximal end is greater than or equal to the distance between the quantitative cavity and the proximal end .

An in vitro detection system includes the microfluidic chip described in the above-mentioned embodiment and a detection mechanism, the detection mechanism is in communication with the quantitative cavity, and the detection mechanism is configured to detect a sample in the quantitative cavity.

Description of the drawings

Figure 1, Figure 2 and Figure 3 are schematic diagrams of the front, back and side of a microfluidic chip according to an embodiment of the application;

4 and 5 are schematic diagrams of the front and back structures of the centrifugal tray matching the microfluidic chip shown in FIG. 1, respectively;

Fig. 6 is a schematic diagram of assembling the microfluidic chip shown in Fig. 1 and the centrifugal tray shown in Fig. 4;

Figure 7-1, Figure 7-2 and Figure 7-3 are schematic diagrams of the flow of the microfluidic chip shown in Figure 1 to separate and quantify the sample solution, and Figure 7-2-1 is a partial enlarged schematic diagram;

Figure 8-1 and Figure 8-2 are schematic diagrams of the detection process using the microfluidic chip shown in Figure 1 respectively.

The reference signs are explained as follows:

10: microfluidic chip, 11: proximal end, 12: card connection part; 13: chip body, 14: transparent cover film, 15: mounting groove;

100: Separation and quantitative unit, 110: Sample loading chamber, 111: Sample loading hole, 112: First air vent, 120: First connecting micro flow channel, 130: Quantitative chamber, 140: First waste liquid chamber, 150: overflow micro channel, 160: second waste liquid cavity, 170: liquid outlet micro channel, 171: permeation hole, 172: capillary channel, 172a, 172b and 172c are different positions of the capillary channel, 173: the fourth connecting micro channel, 180: the quality control chamber, 190: the second connecting micro channel, 191: the second vent hole, 200: the third connecting micro channel;

20: Centrifugal tray, 21: Mounting position, 22: Rotating mounting part, 23: Inspection hole, 24: Quality control hole.

Detailed ways

It should be noted that when an element is referred to as being "disposed on" another element, it can be directly on the other element or a central element may also exist. When an element is considered to be "connected" or "connected with" another element, it can be directly connected to the other element or a central element may exist at the same time.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the technical field of this application. The terminology used in the specification of the application herein is only for the purpose of describing specific embodiments, and is not intended to limit the application. The term "and/or" as used herein includes any and all combinations of one or more related listed items.

1 and FIG. 2, an embodiment of the present application provides a microfluidic chip 10 having a separation and quantification unit 100. The separation and quantification unit 100 includes a sample loading cavity 110, a first connecting micro flow channel 120, a quantification cavity 130, a first waste liquid cavity 140, an overflow micro flow channel 150, and a second waste liquid cavity 160, each cavity The structure of the communicating device is formed in cooperation with the micro flow channel.

The sample loading cavity 110 has a sample loading hole 111. The sample solution can be added into the sample adding cavity 110 from the sample adding hole 111. The quantitative cavity 130 communicates with the sample adding cavity 110 through the first connecting microfluidic channel 120. The quantification cavity 130 is configured to realize the quantification of the solution to be tested. The first waste liquid cavity 140 is in communication with the quantitative cavity 130. The first waste liquid cavity 140 is configured to be filled with excess sample solution and separated fixed impurity precipitation. The second waste liquid cavity 160 communicates with the quantitative cavity 130 through the overflow microchannel 150. The second waste liquid cavity 160 is configured to be filled with excess sample solution.

In this embodiment, the separation and quantitative unit 100 has a proximal end 11 close to the center of rotation during centrifugation. The sample loading cavity 110 is closer to the proximal end 11 than the quantitative cavity 130 is. The quantitative cavity 130 is closer to the proximal end 11 than the first waste liquid cavity 140. When the dosing cavity 130 is filled with liquid, the excess liquid can enter the second waste liquid cavity 160 through the overflow microchannel 150, and the overall distance of the second waste liquid cavity 160 from the proximal end 11 is greater than or equal to the amount The distance between the cavity 130 and the proximal end 11.

After adding the sample solution to the sample adding cavity 110, by rotating and centrifuging, the sample solution can enter the quantitative cavity 130 and the first waste liquid cavity 140 through the first connecting microchannel 120, and gradually fill up the first waste liquid. In the liquid cavity 140 and the quantitative cavity 130, the excess sample solution overflows into the second waste liquid cavity 160 through the overflow microchannel 150, and the solid impurities in the sample solution can be separated from the solution to be tested by centrifugation ( For example, the blood cells in the whole blood sample are separated from the serum (plasma), and solid impurities are centrifuged to precipitate into the first waste liquid cavity 140, and the solution to be tested remains in the quantitative cavity 130 near the proximal end 11. So as to realize the separation and quantification of the sample solution. When detection is needed, a detection mechanism can be used to detect the quantitative solution to be tested in the quantitative cavity 130.

The microfluidic chip 10 needs only one centrifugation after adding the sample solution to separate and quantify the impurities in the sample solution and the target solution to be tested, without excessive centrifugation operations, so the operation is simple, and the waiting time is short. The efficiency of processing is significantly improved.

In an example, the sample injection hole 111 is closer to the proximal end 11 than the connection position of the sample injection cavity 110 and the first connecting microfluidic channel 120, so as to prevent the sample solution from flowing back from the sample injection hole 111 during centrifugation. .

In an example, the sample loading cavity 110 further has a first vent 112, and the first vent 112 is closer to the proximal end 11 than the connection position of the sample loading cavity 110 and the first connecting microfluidic channel 120. By arranging the first vent 112, the air in the sample adding cavity 110 can be effectively discharged in time during sample loading, so as to ensure the smooth progress of sample loading.

In one example, the connection between the sample loading cavity 110 and the first connecting microfluidic channel 120 is in the shape of a funnel, which is more conducive to introducing the sample solution in the sample loading cavity 110 to the quantitative measurement via the first connecting microfluidic channel 120. In the cavity 130.

In one of the embodiments, the microfluidic chip 10 further includes a quality control cavity 180. The quality control cavity 180 communicates with the second waste liquid cavity 160, and the quality control cavity 180 is farther from the proximal end 11 than the second waste liquid cavity 160. The sample solution entering the second waste liquid cavity 160 will flow in after the quantitative cavity 130 is filled with the sample, and the excess sample solution will flow into the mass away from the proximal end 11 in the second waste liquid cavity 160. In the control cavity 180, by observing whether there is liquid in the quality control cavity 180, it can be determined whether the quantitative cavity 130 is filled with the sample solution.

In an example, the microfluidic chip 10 further includes a second connecting microfluidic channel 190 connected to the second waste liquid cavity 160, and the second connecting microfluidic channel 190 is from an end connected to the second waste liquid cavity 160. It gradually extends toward the proximal end 11, and a second vent 191 is provided at the other end. By arranging the second vent 191 on the side of the second waste liquid cavity 160 close to the proximal end 11, when the sample solution overflows into the second waste liquid cavity 160, the second waste liquid cavity can be removed in time. The air in 160 is discharged to ensure the smooth progress of overflow.

In an example, the microfluidic chip 10 further includes a third connecting microfluidic channel 200, and the first waste liquid cavity 140 is in communication with the quantitative cavity 130 through the third connecting microfluidic channel 200. By providing the third connecting micro flow channel 200, the first waste liquid cavity 140 and the quantitative cavity 130 can be separated, and impurities deposited in the first waste liquid cavity 140 are prevented from entering the quantitative cavity 130.

In an example, the microfluidic chip 10 further includes a microfluidic channel 170 for liquid discharge. One end of the liquid outlet microchannel 170 communicates with the quantitative cavity 130, and the other end has a permeation hole 171. The liquid outlet microchannel 170 is configured to lead the quantitatively measured solution in the quantitative cavity 130 from the permeation hole 171 to the detection mechanism.

In an example, the liquid outlet microchannel 170 includes a capillary channel 172. One end of the capillary channel 172 is connected to the third connecting micro channel 200, and the other end is provided with a permeation hole 171. Exemplarily, the capillary flow channel 172 gradually extends from the end connected to the third connecting micro flow channel 200 in a direction close to the proximal end 11 and then extends in a direction away from the proximal end 11 after being bent. The position of the bending apex of the capillary flow channel 172 is closer to the proximal end 11 than the connection position of the quantitative cavity 130 and the first connecting micro flow channel 120. By setting the capillary flow channel 172, the capillary flow channel 172 can function as a "valve". When the sample solution is separated and quantified, the "valve" is closed, and the sample solution to be tested will not break through the bent apex of the capillary flow channel 172. Permeate from the permeation hole 171; in the subsequent detection, under the action of the capillary force of the capillary channel 172, combined with low-speed centrifugation, the solution to be tested quantitatively in the quantitative cavity 130 will be siphoned along the capillary channel 172 It keeps moving forward and can seep from the permeable hole 171 to be detected.

In an example, the liquid outlet microchannel 170 further includes a fourth connecting microchannel 173. One end of the fourth connecting micro channel 173 is connected to the third connecting micro channel 200, and the other end is connected to the capillary channel 172. By providing the fourth connecting micro flow channel 173, it is more convenient to introduce the quantitative solution to be measured in the quantitative cavity 130 into the capillary flow channel 172 during detection. Optionally, the fourth connecting microfluidic channel 173 gradually extends from the end connected to the third connecting microfluidic channel 200 toward the proximal end 11 to be connected to the capillary channel 172.

During the centrifugal separation and quantification of the sample solution, since the centrifugal force is greater than the hair suction in the capillary flow channel 172, the sample solution will not break through the bent apex of the capillary flow channel 172, and the capillary flow channel 172 can act as a "valve" to close In the subsequent detection, under the condition of low-speed centrifugation, the solution to be tested in the quantitative cavity 130 will continue to advance along the capillary flow channel 172 under the action of the hair suction force, and break through the bending apex of the capillary flow channel 172 Position, the "valve" is opened, and under the action of the siphon, the solution to be tested continues to advance and seeps out through the penetration hole 171 to the detection mechanism to be detected.

The capillary flow channel 172 is used as a valve to control the contact reaction between the sample and the detection mechanism, which can replace the traditional water-soluble membrane or valve and other delayed opening mechanisms, making the sampling detection process more stable and reliable, while simplifying the chip assembly process, which is beneficial reduce manufacturing cost.

In an example, each microfluidic chip 10 is provided with a separation and quantification unit 100. The microfluidic chip 10 can have a fan-shaped structure as a whole, but is not limited to a fan-shaped structure, and can be installed on a centrifuge tray and other devices to realize single item detection of samples.

In an example, the microfluidic chip 10 also has a clamping part 12 for mounting on a centrifugal device. The clamping part 12 is clamped on an external centrifugal tray and other devices, and has the functions of positioning and stable assembly.

In an example, the clamping portion 12 may be located at but not limited to the proximal end 11 of the microfluidic chip 10.

As shown in FIG. 3, in an example, the microfluidic chip 10 includes a chip body 13 and a transparent cover film 14 covering the chip body 13. The chip body 13 and the transparent cover film 14 cooperate to form various cavity structures and flow channel structures. Exemplarily, the grooves of each cavity structure and flow channel structure are pre-formed on the chip body 13, as shown in FIG. 2, each hole is opened on the back of the chip body 13, and each cavity structure and flow channel The groove of the structure is opened on the front surface of the chip body 13, and subsequently covered and sealed on the front surface of the chip body 11 by a transparent cover film 12 to complete the packaging of the cavity structure and the flow channel structure, forming a complete cavity structure and Runner structure.

The transparent cover film 14 can be, but is not limited to, transparent tape or transparent pressure-sensitive adhesive. It cooperates with the chip body 13 to form the entire microfluidic chip 10, which is simple to assemble and does not need to use complicated and expensive ultrasonic welding technology. It can be directly bonded. , Can significantly reduce production costs. It can be understood that, in other examples, the microfluidic chip 10 may also be formed by welding with a relatively high-cost ultrasonic welding technology, or be integrally formed with a 3D printing technology.

The present application also provides an in vitro detection system, which includes the above-mentioned microfluidic chip 10 and a detection mechanism. The detection mechanism communicates with the quantitative cavity 130 through a penetration hole 171, and the detection mechanism is configured to detect a sample in the quantitative cavity 130.

In one example, the detection mechanism is a dry chemical test paper. Exemplarily, the dry chemical test paper includes a support layer and a reaction indicator layer and a diffusion layer sequentially stacked on the support layer. The reaction indicator layer contains a reaction reagent and an indicator reagent capable of reacting with the target substance in the sample to be tested, and the diffusion layer passes through The injection port faces the permeation hole 171. It can be understood that in other examples, the detection mechanism is not limited to dry chemical test paper, and may also be various other test paper strips or reactors.

In an example, as shown in FIG. 2, the microfluidic chip 10 is provided with installation grooves 15 around the permeation hole 171 of the separation and quantitative unit 100, and the detection mechanism is embedded in each installation groove 15.

In an example, as shown in Figs. 4, 5, and 6, the in vitro detection system further includes a centrifugal tray 20 for installation on a centrifugal device. The centrifugal tray 20 is provided with a mounting position 21 for placing the microfluidic chip 10.

Exemplarily, the middle part of the centrifugal tray 20 has a rotating mounting part 22. There are multiple mounting positions 21, and the multiple mounting positions 21 are arranged around the rotating mounting portion 22.

In an example, the centrifugal tray 20 is provided with at least one observation hole for observing the status of the microfluidic chip 10 and/or the detection result at the installation position 21. For example, in the illustrated example, the centrifugal tray 20 is provided with a detection hole 23 corresponding to the detection mechanism, and is provided with a quality control hole 24 for observing the state of the quality control cavity 180.

Taking the microfluidic chip shown in FIG. 1 and the centrifugal tray shown in FIG. 4 as an example to detect whole blood samples, the separation and quantification of the sample solution and the detection process will be described in detail below. A plurality of microfluidic chips 10 can be installed on the centrifugal tray 20, and a corresponding number of microfluidic chips 10 can be installed according to the requirements of the test items and samples. For vacancies, blank microfluidic chips 10 can be used to fill in Ensure the basic balance at each position of the centrifugal tray 20.

The process of separating and quantifying whole blood samples by the microfluidic chip 10 can be referred to but not limited to the following:

As shown in FIG. 7-1, a whole blood sample is added to the sample loading cavity 110 through the sample loading hole 111, the centrifuge tray 20 is installed on a detection device with a centrifugal function, the device is turned on, and the centrifuge tray 20 is rotated.

As shown in Figure 7-2, as the centrifugation progresses, the whole blood sample in the sample loading chamber 110 enters the quantitative chamber 130 and the first waste liquid chamber 140 through the first connecting microchannel 120, and the excess whole blood The blood sample enters the quality control cavity 180 and the second waste liquid cavity 160 through the overflow microchannel 150. When a whole blood sample is detected in the quality control cavity 180, it indicates that the quantitative cavity 13 is filled with whole blood. sample. As shown in Figure 7-2-1, since the bending apex of the capillary channel 172 is closer to the proximal end 11 than the top of the quantitative cavity 130, the centrifugal force is greater than the capillary during high-speed centrifugation (such as 4000-6000 rpm). With the hair suction in the flow channel 172, the whole blood sample solution will only enter the section 172a of the capillary flow channel 172, and will not break through the bending apex 172b of the capillary flow channel 172 and flow to the section 172c.

As shown in Figure 7-3, continuing the centrifugation process, the blood cells in the whole blood sample filled in the quantitative cavity 130 will be separated from the serum (plasma) and deposited in the first waste liquid cavity 140, thereby realizing whole blood Separation and quantification of samples.

The process of using the microfluidic chip 10 to detect whole blood samples can be referred to but not limited to the following:

As shown in Figure 8-1 and Figure 8-2, after the quantification of the whole blood sample is completed, the rotation of the centrifuge tray 20 is suspended, and the serum (plasma) in the quantification chamber 130 will be quantified by the capillary flow channel 172. Continue to advance along the capillary channel 172 and break through the bending apex 172b of the capillary channel 172. Preferably, the device is centrifuged at a low speed (such as 1000-2500 rpm), and the serum (plasma) flows from the 172c section of the capillary channel 172. The permeation hole 171 seeps out, and under the siphon action, the serum (plasma) in the quantitative cavity 130 is continuously discharged until all is emptied for detection.

By adopting the form of separating the microfluidic chip and the centrifugal tray, multiple microfluidic chips can be freely assembled on a centrifugal tray to realize the free collocation of test items and test samples, which is beneficial to improve the overall flexibility of the in vitro test system.

Claims

A microfluidic chip has a separation and quantification unit, the separation and quantification unit includes a sample addition cavity, a first connecting microchannel, a quantification cavity, a first waste liquid cavity, an overflow microchannel, and a second waste The liquid cavity; the sample loading cavity has a sample hole; the quantitative cavity communicates with the sample cavity through the first connecting microchannel; the first waste liquid cavity and the quantitative The cavity is in communication; the second waste liquid cavity is in communication with the quantitative cavity through the overflow microchannel;

The separation and quantitative unit has a proximal end closer to the center of rotation during centrifugation; the sample loading cavity is closer to the proximal end than the quantitative cavity; the quantitative cavity is closer to the first waste liquid cavity Close to the proximal end; when the quantitative cavity is filled with liquid, the excess liquid can enter the second waste liquid cavity through the overflow microchannel, and the second waste liquid cavity as a whole The distance from the proximal end is greater than or equal to the distance between the quantitative cavity and the proximal end.
The microfluidic chip of claim 1, wherein the sample injection hole is closer to the proximal end than the connection position of the sample injection cavity and the first connecting microfluidic channel.
The microfluidic chip of claim 1, wherein the sample loading cavity further has a first vent hole, and the first vent hole is larger than the gap between the sample loading cavity and the first connecting microfluidic channel. The connection position is closer to the proximal end.
The microfluidic chip of claim 1, wherein the connection between the sample loading cavity and the first connecting microfluidic channel is in the shape of a funnel.
The microfluidic chip of claim 1, further comprising a quality control cavity, the quality control cavity is in communication with the second waste liquid cavity, and the quality control cavity is smaller than the second waste liquid cavity. The body is far away from the proximal end.
The microfluidic chip according to claim 1, further comprising a second connection microchannel connected to the second waste liquid chamber, the second connection microchannel being separated from the second waste liquid chamber One end of the connection gradually extends toward the proximal end, and a second vent is provided at the other end.
The microfluidic chip according to any one of claims 1 to 6, further comprising a third connecting microfluidic channel, and the first waste liquid cavity is connected to the quantitative cavity through the third connecting microfluidic channel. Connected.
8. The microfluidic chip according to claim 7, further comprising a liquid outlet microchannel, one end of the liquid outlet microchannel is in communication with the quantitative cavity, and the other end has a permeation hole.
The microfluidic chip according to claim 8, wherein the liquid outlet microchannel comprises a capillary channel, one end of the capillary channel is connected to the third connecting microchannel, and the other end is provided with the Penetration hole

The capillary flow channel gradually extends from the end connected to the third connecting micro flow channel in a direction close to the proximal end and is bent and then extends in a direction away from the proximal end; bending of the capillary flow channel The apex position is closer to the proximal end than the connection position of the quantitative cavity and the first connecting microfluidic channel.
The microfluidic chip according to claim 9, wherein the liquid outlet microchannel further comprises a fourth connecting microchannel, and one end of the fourth connecting microchannel is connected to the third connecting microchannel , The other end is connected with the capillary flow channel.
The microfluidic chip according to claim 10, wherein the fourth connecting microfluidic channel gradually extends from an end connected to the third connecting microfluidic channel toward the proximal end to communicate with the capillary Runner connection.
The microfluidic chip according to any one of claims 1 to 6 and 8 to 11, wherein each of the microfluidic chips is provided with one separation and quantification unit.
The microfluidic chip according to claim 12, wherein the microfluidic chip further has a clamping part for mounting on a centrifugal device.
The microfluidic chip according to claim 13, wherein the clamping part is located at the proximal end.
An in vitro detection system, comprising the microfluidic chip according to any one of claims 1-14 and a detection mechanism, wherein the detection mechanism is in communication with the quantitative cavity, and the detection mechanism is configured to detect the quantitative The sample inside the cavity.
The in vitro detection system according to claim 15, further comprising a centrifugal tray for installation on a centrifugal device, and an installation position for placing the microfluidic chip is provided on the centrifugal tray.
The in vitro detection system according to claim 16, wherein the center of the centrifugal tray has a rotating mounting part, there are a plurality of the mounting positions, and the plurality of mounting positions are arranged around the rotating mounting part.
The in vitro detection system according to claim 16, wherein the centrifugal tray is provided with at least one observation hole in the installation position for observing the state of the microfluidic chip and/or the detection result.
The in vitro detection system according to claim 15, wherein the detection mechanism is a dry chemical test paper.
The in vitro detection system according to claim 19, wherein the dry chemical test paper comprises a support layer and a reaction indicator layer and a diffusion layer sequentially stacked on the support layer, and the reaction indicator layer contains The reaction reagent and indicator reagent for the reaction of the target substance in the sample, and the diffusion layer faces the permeation hole through the injection port.
The in vitro detection system according to any one of claims 15 to 20, wherein the microfluidic chip is provided with mounting grooves surrounding the permeation hole of the separation and quantitative unit, and the detection mechanism is embedded in each of the mounting grooves. in.