CN109765391A - A kind of centrifugal test strips chip of multiple determination - Google Patents
A kind of centrifugal test strips chip of multiple determination Download PDFInfo
- Publication number
- CN109765391A CN109765391A CN201910195145.4A CN201910195145A CN109765391A CN 109765391 A CN109765391 A CN 109765391A CN 201910195145 A CN201910195145 A CN 201910195145A CN 109765391 A CN109765391 A CN 109765391A
- Authority
- CN
- China
- Prior art keywords
- layer
- channel layer
- cleaning solution
- prepare liquid
- pool
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention discloses a kind of centrifugal test strips chips of multiple determination, comprising: cover layer, first passage layer, second channel layer and the backplane level set gradually from top to bottom；Wherein, cooperation is sealed between the cover layer, the first passage layer, the second channel layer and the backplane level.The present invention provides a kind of clean hygienes, detection multiple determination centrifugal type microfludic chip accurate, easy to operate；Meet multi objective while detecting, liquid portion in the chip mostly, more much cleaner than independent test strips after reaction；Liquid enters next stage by centrifugation, and guaranteeing that different time enters different liquids by different centrifugal rotational speeds, user transfers to centrifugation detecting instrument to complete follow-up work after adding prepare liquid, cleaning solution, without the waiting time and subsequent manual operation is carried out, improves the degree of automation.
Description
Technical field
The present invention relates to micro-fluidic chip detection technique field, more particularly to a kind of multiple determination is centrifugal
Test strips chip.
Background technique
Micro-fluidic chip is the emerging technology that chemistry or biological sample are operated and detected on chip.It is micro-
Fluidic chip can be integrated into the operating units such as the preparation of sample, reaction, separation and detection one several square centimeters of chip
On, by the manipulation and control to fluid in microchannel network, it is automatically performed analytic process.Since microfluidic chip technology has
Sample volume is small, integrated level is high, Yi Shixian automation control and the characteristics of high throughput analysis, so that being given birth on micro-fluidic chip
It is more convenient, quick, low in cost to change the more conventional analysis sample pre-treatments of operation.
Chromatograph test strip has been widely used in the fields such as clinical diagnosis, food safety, environment measuring.Detection method packet
Coloration, fluorescence, Magnetic testi etc. are included, depending on the marker in bonding pad, and if quantitative detection, such as fluorescence is needed to be measured
Cleaning solution is added dropwise in liquid after reaction, and removal detection zone is not associated with marker, reduces interference.Operator is needed in routine operation
Be added prepare liquid reaction wait for a period of time after, be manually added cleaning solution, then again with visually or easy detection device into
Row result is read, and the degree of automation is poor, influences detection efficiency.
Therefore, how to provide that a kind of clean hygiene, detection be accurate, multiple determination centrifugal type microfludic examination easy to operate
The problem of paper slip chip is those skilled in the art's urgent need to resolve.
Summary of the invention
In view of this, the present invention provides a kind of centrifugal test strips chips of multiple determination, by micro-fluidic chip and layer
It analyses test strips to combine, meets multi objective while detecting, liquid portion in the chip mostly, more much cleaner than independent test strips after reaction；
Liquid enters next stage by centrifugation, and guarantees that different time enters different liquids by different centrifugal rotational speeds, and user is adding
After complete prepare liquid, cleaning solution, transfers to centrifugation detecting instrument to complete follow-up work, without the waiting time and carry out subsequent manual
Operation improves the degree of automation.
To achieve the goals above, the present invention adopts the following technical scheme:
A kind of centrifugal test strips chip of multiple determination, comprising: the cover layer that sets gradually from top to bottom, first passage
Layer, second channel layer and backplane level；Wherein, the cover layer, the first passage layer, the second channel layer and the bottom plate
Cooperation is sealed between layer；
The cover layer be provided with visual window, cover layer ventilation mouth, cover layer prepare liquid sample holes, cover layer cleaning solution into
Sample hole, cover layer prepare liquid venthole, cover layer cleaning solution venthole, cover layer sample inlet pool venthole, cover board chip fixation hole；
The first passage layer includes detection zone through-hole I, detection zone through-hole II, first passage layer prepare liquid separating pool,
One channel layer cleaning solution separating pool, first passage layer prepare liquid feed pathway, first passage layer cleaning solution feed pathway, first are led to
Channel layer sample inlet pool, sample inlet pool venting channels, first passage layer prepare liquid sample holes, first passage layer cleaning solution sample holes, first
Channel layer prepare liquid venthole, first passage layer cleaning solution venthole, first passage layer sample inlet pool venthole, first passage layer core
Piece fixation hole；
The detection zone through-hole I is corresponding with the cover layer ventilation mouth and is connected to；The detection zone through-hole II with it is described can
Form is correspondingly arranged；
The first passage layer prepare liquid feed pathway is connected to the first passage layer prepare liquid separating pool and described first
Channel layer sample inlet pool；The first passage layer cleaning solution feed pathway is connected to the first passage layer cleaning solution separating pool and described
First passage layer sample inlet pool；The first passage layer sample inlet pool venthole is correspondingly arranged with the cover layer sample inlet pool venthole,
And the sample inlet pool venting channels are connected to the first passage layer sample inlet pool venthole and the first passage layer sample inlet pool；
The second channel layer includes detection zone through-hole III, detection zone through-hole IV, second channel layer prepare liquid separating pool,
Two channel layer cleaning solution separating pools, second channel layer prepare liquid main channel, second channel layer cleaning solution main channel, second channel layer
Sample inlet pool, second channel layer prepare liquid sample holes, second channel layer cleaning solution sample holes, second channel layer prepare liquid venthole,
Second channel layer cleaning solution venthole, second channel layer chip fixation hole；
The detection zone through-hole III is correspondingly arranged with the detection zone through-hole I；The detection zone through-hole IV and the detection
Area's through-hole II is correspondingly arranged；
The second channel layer prepare liquid sample holes and the first passage layer prepare liquid sample holes, the cover layer are to be measured
Liquid sample holes are correspondingly arranged, the second channel layer prepare liquid venthole and the first passage layer prepare liquid venthole, described
Cover layer prepare liquid venthole is correspondingly arranged, and the second channel layer prepare liquid main channel be connected to the second channel layer to
Liquid sample holes, the second channel layer prepare liquid separating pool and the second channel layer prepare liquid venthole are surveyed, described second is logical
Channel layer prepare liquid separating pool is corresponding with the first passage layer prepare liquid separating pool and is connected to；
The second channel layer cleaning solution sample holes and the first passage layer cleaning solution sample holes, the cover layer are cleaned
Liquid sample holes are correspondingly arranged, second channel layer cleaning solution venthole and the first passage layer cleaning solution venthole, the cover board
Layer cleaning solution venthole is correspondingly arranged, and the second channel layer cleaning solution main channel is connected to the second channel layer cleaning solution
Sample holes, the second channel layer cleaning solution separating pool and the second channel layer cleaning solution venthole, the second channel layer
Cleaning solution separating pool is corresponding with the first passage layer cleaning solution separating pool and is connected to；The second channel layer sample inlet pool with it is described
First passage layer sample inlet pool is correspondingly arranged and is connected to；
The backplane level is provided with chromatograph test strip and bottom plate chip fixation hole, wherein the chromatograph test strip is by sample
Pad, nitrocellulose filter, bonding pad and absorption pad composition, wherein the nitrocellulose filter be located at centre, the sample pad and
The absorption pad is located at both ends；The bonding pad is between the sample pad and the nitrocellulose filter；
The chromatograph test strip has been arranged radially several, and each chromatograph test strip matches setting respectively
There are the corresponding second channel layer sample inlet pool, the first passage layer sample inlet pool, the first passage layer prepare liquid feed liquor logical
Road, the first passage layer prepare liquid separating pool, the second channel layer prepare liquid separating pool, the first passage layer cleaning solution
Feed pathway, the first passage layer cleaning solution separating pool, the second channel layer cleaning solution separating pool, sample inlet pool ventilation
Channel, the first passage layer sample inlet pool venthole, the cover layer sample inlet pool venthole, the detection zone through-hole I, the inspection
Survey area's through-hole II, the detection zone through-hole III and the detection zone through-hole IV；
The sample pad of the chromatograph test strip is correspondingly arranged with the second channel layer sample inlet pool, and described second
Channel layer sample inlet pool is connected to the backplane level, and liquid can be contacted with the sample pad in the second channel layer sample inlet pool,
So that liquid in the second channel layer sample inlet pool is flowed in the sample pad, the combination is stepped by chromatography effect
Pad, the nitrocellulose filter, the absorption pad carry out series reaction；The nitrocellulose of the chromatograph test strip
Film is correspondingly arranged with the detection zone through-hole IV, the detection zone through-hole II, the visual window, by the visual window and described
The chromatograph test strip reactiveness can be observed in detection zone through-hole IV, the detection zone through-hole II；The absorption pad with it is described
Detection zone through-hole III, the detection zone through-hole I, cover layer ventilation mouth are correspondingly arranged.
Preferably, in a kind of above-mentioned centrifugal test strips chip of multiple determination, the visual window is open type through-hole.
Preferably, in a kind of above-mentioned centrifugal test strips chip of multiple determination, the first passage layer cleaning solution into
The shape in liquid channel includes but are not limited to waveform, and waveform channel can increase passage length, can also be fold-line-shaped or
Zigzag.
Preferably, in a kind of above-mentioned centrifugal test strips chip of multiple determination, the cover board chip fixation hole and institute
State the fixed hole shape of first passage layer chip fixation hole, the second channel layer chip fixation hole, the bottom plate chip it is identical and
It is overlapped setting, is formed and runs through a kind of chip fixation hole of the centrifugal test strips chip of multiple determination.In centrifugal process
The centrifugal test strips chip of a kind of multiple determination is fixed on centrifugation detector by the chip fixation hole, and described one
The kind centrifugal test strips chip of multiple determination is centrifuged by center of circle rotation of the chip fixation hole.
Preferably, in a kind of above-mentioned centrifugal test strips chip of multiple determination, the first passage layer cleaning solution point
Liquid pool, the second channel layer cleaning solution separating pool, the first passage layer prepare liquid separating pool, the second channel layer are to be measured
Liquid separating pool is respectively positioned on the second channel layer sample inlet pool close to the side of the chip fixation hole.It is described in centrifugal process
First passage layer cleaning solution separating pool, the second channel layer cleaning solution separating pool, the first passage layer prepare liquid separating pool,
Liquid is flow under the influence of centrifugal force in the second channel layer sample inlet pool in the second channel layer prepare liquid separating pool.
Preferably, in a kind of above-mentioned centrifugal test strips chip of multiple determination, the second channel layer cleaning solution master
Channel, the second channel layer cleaning solution separating pool, the second channel layer prepare liquid main channel, the second channel layer are to be measured
Liquid separating pool, the second channel layer sample inlet pool are by closely to being far successively arranged in chip fixation hole side.
Preferably, in a kind of above-mentioned centrifugal test strips chip of multiple determination, a kind of multiple determination centrifugation
The driving force of liquid is centrifugal force in formula test strips chip.
Preferably, in a kind of above-mentioned centrifugal test strips chip of multiple determination, second channel layer prepare liquid separating pool,
Second channel layer cleaning solution separating pool, second channel layer prepare liquid main channel, second channel layer cleaning solution main channel and the bottom
The isolation of plate layer, the liquid being located therein will not flow in the backplane level.
Preferably, in a kind of above-mentioned centrifugal test strips chip of multiple determination, the second channel layer prepare liquid master
Channel, second channel layer cleaning solution main channel are the pipeline being built in the second channel layer；The first passage layer is to be measured
Liquid feed pathway, first passage layer cleaning solution feed pathway are the pipeline being built in the first passage layer.
Preferably, it in a kind of above-mentioned multiple determination centrifugal type microfludic test paper chip, is provided in the bonding pad
Marker reacts the prepare liquid with marker.
When liquid feeding, prepare liquid is added by cover layer prepare liquid sample holes, through to first passage layer prepare liquid sample holes, the
Two channel layer prepare liquid sample holes, second channel layer prepare liquid main channel are full of first passage layer prepare liquid separating pool and one by one
Two channel layer prepare liquid separating pools, and pass through second channel layer prepare liquid venthole, first passage layer prepare liquid venthole and lid
Plate layer prepare liquid venthole is exhausted；Cleaning solution is added by cover layer cleaning solution sample holes, through being cleaned to first passage layer
Liquid sample holes, second channel layer cleaning solution sample holes, second channel layer cleaning solution main channel are full of the cleaning of first passage layer one by one
Liquid separating pool and second channel layer cleaning solution separating pool, and cleaned by second channel layer cleaning solution venthole, first passage layer
Liquid venthole and cover layer cleaning solution venthole are exhausted；
It can be seen via above technical scheme that compared with prior art, the present disclosure provides a kind of multiple determinations
Centrifugal type microfludic test paper chip meets multi objective while detecting by micro-fluidic chip in conjunction with chromatograph test strip, improves work
Efficiency, liquid portion in the chip mostly, more much cleaner than independent test strips after reaction；Liquid enters next stage by centrifugation, and logical
It crosses different centrifugal rotational speeds and guarantees that different time enters different liquids, user transfers to centrifugation to examine after adding prepare liquid, cleaning solution
It surveys instrument and completes follow-up work, without the waiting time and carry out subsequent manual operation, improve the degree of automation.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
The embodiment of invention for those of ordinary skill in the art without creative efforts, can also basis
The attached drawing of offer obtains other attached drawings.
Fig. 1 attached drawing is three-dimensional explosive view of the invention；
Fig. 2 attached drawing is cover layer structure chart；
Fig. 3 attached drawing is first passage layer structure chart；
Fig. 4 attached drawing is second channel layer structure chart；
Fig. 5 attached drawing is bottom plate schematic diagram of a layer structure；
Fig. 6 attached drawing is chromatograph test strip structural schematic diagram.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
The embodiment of the invention discloses a kind of centrifugal test strips chips of multiple determination, and micro-fluidic chip and chromatography are tried
Paper slip combines, and meets multi objective while detecting, raising working efficiency, liquid portion in the chip mostly after reaction, than independent test paper
Item is much cleaner；Liquid enters next stage by centrifugation, and guarantees that different time enters different liquids by different centrifugal rotational speeds,
User transfers to centrifugation detecting instrument to complete follow-up work, without the waiting time and carries out after adding prepare liquid, cleaning solution
Subsequent manual operation, improves the degree of automation.
A kind of centrifugal test strips chip of multiple determination, comprising: the cover layer 1, first set gradually from top to bottom is logical
Channel layer 2, second channel layer 3 and backplane level 4；Wherein, between cover layer 1, first passage layer 2, second channel layer 3 and backplane level 4
Sealing cooperation；
Cover layer 1 is provided with visual window 11, cover layer ventilation mouth 12, cover layer prepare liquid sample holes 13, cover layer cleaning
Liquid sample holes 16, cover layer prepare liquid venthole 15, cover layer cleaning solution venthole 14, cover layer sample inlet pool venthole 17, lid
Board chip fixation hole 18；
First passage layer 2 include detection zone through-hole I 21, detection zone through-hole II 22, first passage layer prepare liquid separating pool 23,
First passage layer cleaning solution separating pool 24, first passage layer prepare liquid feed pathway 25, first passage layer cleaning solution feed pathway
26, first passage layer sample inlet pool 27, sample inlet pool venting channels 28, first passage layer prepare liquid sample holes 29, first passage layer are clear
Washing lotion sample holes 212, first passage layer prepare liquid venthole 211, first passage layer cleaning solution venthole 210, first passage layer
Sample inlet pool venthole 213, first passage layer chip fixation hole 214；
Detection zone through-hole I 21 is corresponding with cover layer ventilation mouth 12 and is connected to；Detection zone through-hole II 22 is corresponding with visual window 11
Setting；
First passage layer prepare liquid feed pathway 25 be connected to first passage layer prepare liquid separating pool 23 and first passage layer into
Sample pond 27；First passage layer cleaning solution feed pathway 26 is connected to first passage layer cleaning solution separating pool 24 and first passage layer sample introduction
Pond 27；First passage layer sample inlet pool venthole 213 is correspondingly arranged with cover layer sample inlet pool venthole 17, and sample inlet pool ventilation is logical
Road 28 is connected to first passage layer sample inlet pool venthole 213 and first passage layer sample inlet pool 27；
Second channel layer 3 includes detection zone through-hole III 31, detection zone through-hole IV 32, second channel layer prepare liquid separating pool
33, second channel layer cleaning solution separating pool 312, second channel layer prepare liquid main channel 34, second channel layer cleaning solution main channel
35, second channel layer sample inlet pool 36, second channel layer prepare liquid sample holes 37, second channel layer cleaning solution sample holes 310, second
Channel layer prepare liquid venthole 39, second channel layer cleaning solution venthole 38, second channel layer chip fixation hole 311；
Detection zone through-hole III 31 is correspondingly arranged with detection zone through-hole I 21；Detection zone through-hole IV 32 and detection zone through-hole II 22
It is correspondingly arranged；
Second channel layer prepare liquid sample holes 37 and first passage layer prepare liquid sample holes 29, cover layer prepare liquid sample holes
13 are correspondingly arranged, and second channel layer prepare liquid venthole 39 and first passage layer prepare liquid venthole 211, cover layer prepare liquid are logical
Stomata 15 is correspondingly arranged, and second channel layer prepare liquid main channel 34 is connected to second channel layer prepare liquid sample holes 37, second
Channel layer prepare liquid separating pool 33 and second channel layer prepare liquid venthole 39, second channel layer prepare liquid separating pool 33 and first
Channel layer prepare liquid separating pool 23 is corresponding and is connected to；
Second channel layer cleaning solution sample holes 310 and first passage layer cleaning solution sample holes 212, cover layer cleaning solution sample introduction
Hole 16 is correspondingly arranged, second channel layer cleaning solution venthole 38 and first passage layer cleaning solution venthole 210, cover layer cleaning solution
Venthole 14 is correspondingly arranged, and second channel layer cleaning solution main channel 35 is connected to second channel layer cleaning solution sample holes 310, the
Two channel layer cleaning solution separating pools 312 and second channel layer cleaning solution venthole 38, second channel layer cleaning solution separating pool 312 with
First passage layer cleaning solution separating pool 24 is corresponding and is connected to；Second channel layer sample inlet pool 36 is corresponding with first passage layer sample inlet pool 27
It is arranged and is connected to；
Backplane level 4 is provided with chromatograph test strip 41 and bottom plate chip fixation hole 42, and wherein chromatograph test strip 41 is by sample pad
43, nitrocellulose filter 44, bonding pad 45 and absorption pad 46 form, and wherein nitrocellulose filter 44 is located at centre, sample pad 43
Both ends are located at absorption pad 46；Bonding pad 45 is between sample pad 43 and nitrocellulose filter 44；
Chromatograph test strip 41 has been arranged radially several, and each chromatograph test strip 41 matches be provided with phase respectively
Second channel layer sample inlet pool 36, the first passage layer sample inlet pool 27, first passage layer prepare liquid feed pathway 25, first passage answered
Layer prepare liquid separating pool 23, second channel layer prepare liquid separating pool 33, first passage layer cleaning solution feed pathway 26, first passage
Layer cleaning solution separating pool 24, second channel layer cleaning solution separating pool 312, sample inlet pool venting channels 28, first passage layer sample inlet pool
Venthole 213, cover layer sample inlet pool venthole 17, detection zone through-hole I 21, detection zone through-hole II 22, III 31 and of detection zone through-hole
Detection zone through-hole IV 32；
The sample pad 43 of chromatograph test strip 41 is correspondingly arranged with second channel layer sample inlet pool 36, and second channel layer sample introduction
Pond 36 is connected to backplane level 4, and liquid can be contacted with sample pad 43 in second channel layer sample inlet pool 36, makes second channel layer sample introduction
Liquid can flow in sample pad 43 in pond 36, step into bonding pad 45, nitrocellulose filter 44, absorption pad by chromatography effect
46, carry out series reaction；The nitrocellulose filter 44 of chromatograph test strip 4 and detection zone through-hole IV 32, detection zone through-hole II 22,
Visual window 11 is correspondingly arranged, and chromatographic test paper can be observed by visual window 11 and detection zone through-hole IV 32, detection zone through-hole II 22
41 reactiveness of item；Absorption pad 46 is correspondingly arranged with detection zone through-hole III 31, detection zone through-hole I 21, cover layer ventilation mouth 12.
In order to further optimize the above technical scheme, visual window 11 is open type through-hole.
In order to further optimize the above technical scheme, the shape of first passage layer cleaning solution feed pathway 26 includes but not only
It is limited to waveform, waveform channel can increase passage length, can also be fold-line-shaped or zigzag.
In order to further optimize the above technical scheme, cover board chip fixation hole 18 and first passage layer chip fixation hole 214,
Second channel layer chip fixation hole 311, bottom plate chip 42 shapes of fixation hole are identical and are overlapped setting, are formed and run through a kind of multi objective
Detect the chip fixation hole of centrifugal test strips chip.A kind of centrifugal test strips chip of multiple determination is logical in centrifugal process
It crosses chip fixation hole to be fixed on centrifugation detector, a kind of centrifugal test strips chip of multiple determination is circle with chip fixation hole
Heart rotation is centrifuged.
In order to further optimize the above technical scheme, first passage layer cleaning solution separating pool 24, second channel layer cleaning solution
Separating pool 312, first passage layer prepare liquid separating pool 23, second channel layer prepare liquid separating pool 33 be respectively positioned on second channel layer into
Sample pond 36 is close to the side of chip fixation hole.In centrifugal process, first passage layer cleaning solution separating pool 24, second channel layer are clear
Washing lotion separating pool 312, first passage layer prepare liquid separating pool 23, in second channel layer prepare liquid separating pool 33 liquid in centrifugal force
Under the action of flow in second channel layer sample inlet pool 36.
In order to further optimize the above technical scheme, second channel layer cleaning solution main channel 35, second channel layer cleaning solution
Separating pool 312, second channel layer prepare liquid main channel 34, second channel layer prepare liquid separating pool 33, second channel layer sample inlet pool
36 by closely to being far successively arranged in chip fixation hole side.
In order to further optimize the above technical scheme, in a kind of centrifugal test strips chip of multiple determination liquid driving
Power is centrifugal force.
In order to further optimize the above technical scheme, second channel layer prepare liquid separating pool 33, second channel layer cleaning solution
Separating pool 312, second channel layer prepare liquid main channel 34, second channel layer cleaning solution main channel 35 and backplane level 4 completely cut off, and are located at
Liquid therein will not flow in backplane level 4.
In order to further optimize the above technical scheme, second channel layer prepare liquid main channel 34, second channel layer cleaning solution
Main channel 35 is the pipeline being built in second channel layer 3；First passage layer prepare liquid feed pathway 25, the cleaning of first passage layer
Liquid feed pathway 26 is the pipeline being built in first passage layer 2.
In order to further optimize the above technical scheme, it is provided with marker in bonding pad 45, keeps prepare liquid anti-with marker
It answers.
When liquid feeding, prepare liquid is added by cover layer prepare liquid sample holes 13, through to first passage layer prepare liquid sample holes
29, second channel layer prepare liquid sample holes 37, second channel layer prepare liquid main channel 34 are full of first passage layer prepare liquid one by one
Separating pool 23 and second channel layer prepare liquid separating pool 33, and pass through second channel layer prepare liquid venthole 39, first passage layer
Prepare liquid venthole 211 and cover layer prepare liquid venthole 15 are exhausted；By cleaning solution by cover layer cleaning solution sample holes 16
It is added, through to first passage layer cleaning solution sample holes 212, second channel layer cleaning solution sample holes 310, second channel layer cleaning solution
Main channel 35 is full of first passage layer cleaning solution separating pool 24 and second channel layer cleaning solution separating pool 312 one by one, and passes through the
Two channel layer cleaning solution ventholes 38, first passage layer cleaning solution venthole 210 and cover layer cleaning solution venthole 14 are arranged
Gas；
A kind of centrifugal test strips chip of multiple determination is fixed on the rotary shaft of centrifugation detector by chip fixation hole
On, it is centrifuged a kind of centrifugal test strips chip of multiple determination with the rotation of the chip fixation hole center of circle, liquid flows over
Journey is as follows:
(1) slow-speed of revolution is centrifuged, and prepare liquid is by first passage layer prepare liquid separating pool 23 and second channel layer prepare liquid liquid separation
Pond 33 enters first passage layer sample inlet pool 27 and second channel layer sample inlet pool 36 through first passage layer prepare liquid feed pathway 25；
(2) after centrifugation detector low speed effect, static, first passage layer sample inlet pool 27 and second channel layer sample inlet pool 36
The sample pad 43 of interior prepare liquid contact chromatograph test strip 41, by chromatography effect step into bonding pad 45, nitrocellulose filter 44,
Absorption pad 46 carries out series reaction, and prepare liquid reacted with marker in bonding pad 45, by cover layer ventilation mouth 12,
The gas in 41 place space of chromatograph test strip is discharged for detection zone through-hole I 21, detection zone through-hole III 31, equilibrium air pressure；
(3) high speed centrifugation, cleaning solution is by first passage layer cleaning solution separating pool 24 and second channel layer cleaning solution separating pool
312 enter first passage layer sample inlet pool 27 and second channel layer sample inlet pool 36 through first passage layer cleaning solution feed pathway 26；
(4) static after being centrifuged detector high velocity effect, first passage layer sample inlet pool 27 and second channel layer sample inlet pool 36
The sample pad 43 that cleaning liquid inside contacts chromatograph test strip 41 enters bonding pad 45 by chromatography effect, nitrocellulose filter 44, absorbs
Remnants on nitrocellulose filter 44 are not associated with reagent and removed by pad 46, and interference when result being avoided to read is saturating by cover layer
The gas in 41 place space of chromatograph test strip is discharged for port 12, detection zone through-hole I 21, detection zone through-hole III 31, equilibrium air pressure.
(5) it is observed by detection zone through-hole II 22, detection zone through-hole IV 32, visual window 11 anti-on nitrocellulose filter 44
Answer result.
Each embodiment in this specification is described in a progressive manner, the highlights of each of the examples are with other
The difference of embodiment, the same or similar parts in each embodiment may refer to each other.For device disclosed in embodiment
For, since it is corresponded to the methods disclosed in the examples, so being described relatively simple, related place is said referring to method part
It is bright.
The foregoing description of the disclosed embodiments enables those skilled in the art to implement or use the present invention.
Various modifications to these embodiments will be readily apparent to those skilled in the art, as defined herein
General Principle can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, of the invention
It is not intended to be limited to the embodiments shown herein, and is to fit to and the principles and novel features disclosed herein phase one
The widest scope of cause.
Claims (10)
1. a kind of centrifugal test strips chip of multiple determination characterized by comprising the cover board set gradually from top to bottom
Layer, first passage layer, second channel layer and backplane level；Wherein, the cover layer, the first passage layer, the second channel
Cooperation is sealed between layer and the backplane level；
The cover layer is provided with visual window, cover layer ventilation mouth, cover layer prepare liquid sample holes, cover layer cleaning solution sample introduction
Hole, cover layer prepare liquid venthole, cover layer cleaning solution venthole, cover layer sample inlet pool venthole, cover board chip fixation hole；
The first passage layer includes detection zone through-hole I, detection zone through-hole II, first passage layer prepare liquid separating pool, first logical
Channel layer cleaning solution separating pool, first passage layer prepare liquid feed pathway, first passage layer cleaning solution feed pathway, first passage layer
Sample inlet pool, sample inlet pool venting channels, first passage layer prepare liquid sample holes, first passage layer cleaning solution sample holes, first passage
Layer prepare liquid venthole, first passage layer cleaning solution venthole, first passage layer sample inlet pool venthole, first passage layer chip are solid
Determine hole；
The detection zone through-hole I is corresponding with the cover layer ventilation mouth and is connected to；The detection zone through-hole II and the visual window
It is correspondingly arranged；
The first passage layer prepare liquid feed pathway is connected to the first passage layer prepare liquid separating pool and the first passage
Layer sample inlet pool；The first passage layer cleaning solution feed pathway is connected to the first passage layer cleaning solution separating pool and described first
Channel layer sample inlet pool；The first passage layer sample inlet pool venthole is correspondingly arranged with the cover layer sample inlet pool venthole, and
The sample inlet pool venting channels are connected to the first passage layer sample inlet pool venthole and the first passage layer sample inlet pool；
The second channel layer includes detection zone through-hole III, detection zone through-hole IV, second channel layer prepare liquid separating pool, second logical
Channel layer cleaning solution separating pool, second channel layer prepare liquid main channel, second channel layer cleaning solution main channel, second channel layer sample introduction
Pond, second channel layer prepare liquid sample holes, second channel layer cleaning solution sample holes, second channel layer prepare liquid venthole, second
Channel layer cleaning solution venthole, second channel layer chip fixation hole；
The detection zone through-hole III is correspondingly arranged with the detection zone through-hole I；The detection zone through-hole IV and the detection zone are logical
Hole II is correspondingly arranged；
The second channel layer prepare liquid sample holes and the first passage layer prepare liquid sample holes, the cover layer prepare liquid into
Sample hole is correspondingly arranged, the second channel layer prepare liquid venthole and the first passage layer prepare liquid venthole, the cover board
Layer prepare liquid venthole is correspondingly arranged, and the second channel layer prepare liquid main channel is connected to the second channel layer prepare liquid
Sample holes, the second channel layer prepare liquid separating pool and the second channel layer prepare liquid venthole, the second channel layer
Prepare liquid separating pool is corresponding with the first passage layer prepare liquid separating pool and is connected to；
The second channel layer cleaning solution sample holes and the first passage layer cleaning solution sample holes, the cover layer cleaning solution into
Sample hole is correspondingly arranged, and second channel layer cleaning solution venthole and the first passage layer cleaning solution venthole, the cover layer are clear
Washing lotion venthole is correspondingly arranged, and the second channel layer cleaning solution main channel is connected to the second channel layer cleaning solution sample introduction
Hole, the second channel layer cleaning solution separating pool and the second channel layer cleaning solution venthole, the second channel layer cleaning
Liquid separating pool is corresponding with the first passage layer cleaning solution separating pool and is connected to；The second channel layer sample inlet pool and described first
Channel layer sample inlet pool is correspondingly arranged and is connected to；
The backplane level is provided with chromatograph test strip and bottom plate chip fixation hole, wherein the chromatograph test strip is by sample pad, nitre
Acid cellulose film, bonding pad and absorption pad composition, wherein the nitrocellulose filter is located at centre, the sample pad and the suction
It receives pad and is located at both ends；The bonding pad is between the sample pad and the nitrocellulose filter；
The chromatograph test strip has been arranged radially several, and each chromatograph test strip matches be provided with phase respectively
The second channel layer sample inlet pool, the first passage layer sample inlet pool, the first passage layer prepare liquid feed pathway, institute answered
State first passage layer prepare liquid separating pool, the second channel layer prepare liquid separating pool, the first passage layer cleaning solution feed liquor
Channel, the first passage layer cleaning solution separating pool, the second channel layer cleaning solution separating pool, sample inlet pool ventilation are logical
Road, the first passage layer sample inlet pool venthole, the cover layer sample inlet pool venthole, the detection zone through-hole I, the detection
Area's through-hole II, the detection zone through-hole III and the detection zone through-hole IV；
The sample pad of the chromatograph test strip is correspondingly arranged with the second channel layer sample inlet pool, and the second channel
Layer sample inlet pool is connected to the backplane level, and liquid can be contacted with the sample pad in the second channel layer sample inlet pool；It is described
The nitrocellulose filter of chromatograph test strip and the detection zone through-hole IV, the detection zone through-hole II, the visual window pair
It should be arranged；The absorption pad is correspondingly arranged with the detection zone through-hole III, the detection zone through-hole I, cover layer ventilation mouth.
2. the centrifugal test strips chip of a kind of multiple determination according to claim 1, which is characterized in that the visual window
For open type through-hole.
3. the centrifugal test strips chip of a kind of multiple determination according to claim 1, which is characterized in that described first is logical
The shape of channel layer cleaning solution feed pathway includes but are not limited to waveform.
4. the centrifugal test strips chip of a kind of multiple determination according to claim 1, which is characterized in that the cover board core
Piece fixation hole is fixed with the first passage layer chip fixation hole, the second channel layer chip fixation hole, the bottom plate chip
Hole shape is identical and is overlapped setting, is formed and runs through a kind of chip fixation hole of the centrifugal test strips chip of multiple determination.
5. the centrifugal test strips chip of a kind of multiple determination according to claim 4, which is characterized in that described first is logical
Channel layer cleaning solution separating pool, the second channel layer cleaning solution separating pool, the first passage layer prepare liquid separating pool, described
Two channel layer prepare liquid separating pools are respectively positioned on the second channel layer sample inlet pool close to the side of the chip fixation hole.
6. the centrifugal test strips chip of a kind of multiple determination according to claim 4, which is characterized in that described second is logical
Channel layer cleaning solution main channel, the second channel layer cleaning solution separating pool, the second channel layer prepare liquid main channel, described
Two channel layer prepare liquid separating pools, the second channel layer sample inlet pool are by closely to being far successively arranged in the chip fixation hole one
Side.
7. the centrifugal test strips chip of a kind of multiple determination according to claim 1, which is characterized in that described one kind is more
The driving force of liquid is centrifugal force in the centrifugal test strips chip of Indexs measure.
8. the centrifugal test strips chip of a kind of multiple determination according to claim 1, which is characterized in that second channel layer
Prepare liquid separating pool, second channel layer cleaning solution separating pool, second channel layer prepare liquid main channel, second channel layer cleaning solution master
Channel and the backplane level completely cut off.
9. the centrifugal test strips chip of a kind of multiple determination according to claim 1, which is characterized in that described second is logical
Channel layer prepare liquid main channel, second channel layer cleaning solution main channel are the pipeline being built in the second channel layer；Described
One channel layer prepare liquid feed pathway, first passage layer cleaning solution feed pathway are the pipe being built in the first passage layer
Road.
10. the centrifugal test strips chip of a kind of multiple determination according to claim 1, which is characterized in that the combination
Marker is provided in pad.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910195145.4A CN109765391B (en) | 2019-03-14 | 2019-03-14 | Multi-index detection centrifugal test strip chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910195145.4A CN109765391B (en) | 2019-03-14 | 2019-03-14 | Multi-index detection centrifugal test strip chip |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109765391A true CN109765391A (en) | 2019-05-17 |
| CN109765391B CN109765391B (en) | 2023-04-28 |
Family
ID=66459195
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910195145.4A Active CN109765391B (en) | 2019-03-14 | 2019-03-14 | Multi-index detection centrifugal test strip chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109765391B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110568199A (en) * | 2019-09-12 | 2019-12-13 | 重庆科技学院 | Multi-channel fluorescence immunochromatography detection micro-fluidic chip |
| CN110568203A (en) * | 2019-09-12 | 2019-12-13 | 重庆科技学院 | Application method of multi-channel fluorescence immunochromatographic microfluidic chip |
| DE102022106693B3 (en) | 2022-03-22 | 2023-03-16 | Dermagnostix GmbH | Centrifugal Microfluidic Biochip |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011064474A (en) * | 2009-09-15 | 2011-03-31 | Toppan Printing Co Ltd | Sample analysis chip, and sample analyzer and sample analysis method using the chip |
| CN104502617A (en) * | 2014-12-24 | 2015-04-08 | 杭州霆科生物科技有限公司 | Full-automatic and high-throughput micro-fluidic chip system and method for detecting pesticide residue |
| CN106513063A (en) * | 2016-06-06 | 2017-03-22 | 苏州汶颢芯片科技有限公司 | Centrifugal chip capable of achieving sequential reactions and mixing method thereof |
| CN206229378U (en) * | 2016-08-24 | 2017-06-09 | 杭州霆科生物科技有限公司 | A kind of centrifugal routine urinalysis detection micro-fluidic chip |
| CN107739706A (en) * | 2017-09-26 | 2018-02-27 | 南京岚煜生物科技有限公司 | The micro-fluidic nucleic acid detection chip of more flux and its application method of active control stream |
| CN109212201A (en) * | 2017-07-03 | 2019-01-15 | 杭州霆科生物科技有限公司 | A kind of centrifugal type microfludic chip for hepatitis B five detections in serum |
| CN110568199A (en) * | 2019-09-12 | 2019-12-13 | 重庆科技学院 | Multi-channel fluorescence immunochromatography detection micro-fluidic chip |
-
2019
- 2019-03-14 CN CN201910195145.4A patent/CN109765391B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011064474A (en) * | 2009-09-15 | 2011-03-31 | Toppan Printing Co Ltd | Sample analysis chip, and sample analyzer and sample analysis method using the chip |
| CN104502617A (en) * | 2014-12-24 | 2015-04-08 | 杭州霆科生物科技有限公司 | Full-automatic and high-throughput micro-fluidic chip system and method for detecting pesticide residue |
| CN106513063A (en) * | 2016-06-06 | 2017-03-22 | 苏州汶颢芯片科技有限公司 | Centrifugal chip capable of achieving sequential reactions and mixing method thereof |
| CN206229378U (en) * | 2016-08-24 | 2017-06-09 | 杭州霆科生物科技有限公司 | A kind of centrifugal routine urinalysis detection micro-fluidic chip |
| CN109212201A (en) * | 2017-07-03 | 2019-01-15 | 杭州霆科生物科技有限公司 | A kind of centrifugal type microfludic chip for hepatitis B five detections in serum |
| CN107739706A (en) * | 2017-09-26 | 2018-02-27 | 南京岚煜生物科技有限公司 | The micro-fluidic nucleic acid detection chip of more flux and its application method of active control stream |
| CN110568199A (en) * | 2019-09-12 | 2019-12-13 | 重庆科技学院 | Multi-channel fluorescence immunochromatography detection micro-fluidic chip |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110568199A (en) * | 2019-09-12 | 2019-12-13 | 重庆科技学院 | Multi-channel fluorescence immunochromatography detection micro-fluidic chip |
| CN110568203A (en) * | 2019-09-12 | 2019-12-13 | 重庆科技学院 | Application method of multi-channel fluorescence immunochromatographic microfluidic chip |
| CN110568203B (en) * | 2019-09-12 | 2022-05-24 | 重庆科技学院 | Application method of multi-channel fluorescence immunochromatographic microfluidic chip |
| CN110568199B (en) * | 2019-09-12 | 2022-05-31 | 重庆科技学院 | Multi-channel fluorescence immunochromatography detection microfluidic chip |
| DE102022106693B3 (en) | 2022-03-22 | 2023-03-16 | Dermagnostix GmbH | Centrifugal Microfluidic Biochip |
| WO2023179810A1 (en) | 2022-03-22 | 2023-09-28 | Dermagnostix GmbH | Centrifugal microfluidic biochip |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109765391B (en) | 2023-04-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP6347861B2 (en) | Test cartridge with integrated transfer module | |
| US4918025A (en) | Self contained immunoassay element | |
| US5399486A (en) | Disposable unit in diagnostic assays | |
| CN109765391A (en) | A kind of centrifugal test strips chip of multiple determination | |
| CN109759155A (en) | A kind of multiple determination centrifugal type microfludic chip | |
| CN109894166A (en) | A kind of mycotoxin multiple determination micro-fluidic chip | |
| US20080268464A1 (en) | Process And Device For Determining The Activity Of Enzymes In Liquids, Or The Concentration And/Or Activity Of Inhibitors In Liquids | |
| CN107199061A (en) | A kind of multitask full-automatic biochemical detection chip | |
| JPS60115865A (en) | Method and structure for detecting and measuring ligand | |
| CN111774104A (en) | Micro-fluidic chip and in-vitro detection device | |
| WO2011029794A1 (en) | Multiplexed analyte concentration measurement | |
| CN209680127U (en) | A kind of multiple determination centrifugal type microfludic test paper chip | |
| CN108627382B (en) | Sample pretreatment and detection integrated device for determining various indexes of soil based on microfluidic chip technology | |
| WO2021077591A1 (en) | Microfluidic chip and in vitro test system | |
| KR101190191B1 (en) | Sampling and dispensing device with integrated filtering device | |
| CN206132630U (en) | Total cholesterol content testing kit | |
| CN107966578A (en) | A kind of medical quick biochemical detection system and detection method | |
| CN209680126U (en) | A kind of multiple determination centrifugal type microfludic chip | |
| CN209772148U (en) | Centrifugal micro-fluidic test paper chip | |
| CN109759156A (en) | A kind of centrifugal type microfludic chip | |
| CN209772147U (en) | Centrifugal micro-fluidic chip | |
| CN108614105A (en) | A kind of reagent strip and its application | |
| CN210045262U (en) | Cardiovascular emergency detection micro-fluidic chip | |
| KR20220046751A (en) | System for single or multi conducting Biochemistry, Immunoassay, Blood test and Gene analysis, and Multi-Disc therefor | |
| CN109894165A (en) | A kind of three joint inspection micrometer fluidic chip of clenbuterol hydrochloride |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |