CN209680127U - A kind of multiple determination centrifugal type microfludic test paper chip - Google Patents
A kind of multiple determination centrifugal type microfludic test paper chip Download PDFInfo
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- CN209680127U CN209680127U CN201920324323.4U CN201920324323U CN209680127U CN 209680127 U CN209680127 U CN 209680127U CN 201920324323 U CN201920324323 U CN 201920324323U CN 209680127 U CN209680127 U CN 209680127U
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Abstract
The utility model discloses a kind of multiple determination centrifugal type microfludic test paper chip, including cover layer, channel layer and backplane level, cover layer and backplane level respectively correspond and are arranged at the top and bottom of channel layer, seal and cooperate with channel layer；Cover layer includes visual window, prepare liquid sample holes, cleaning solution sample holes, venthole and cover board chip fixation hole；Backplane level includes chromatograph test strip and bottom plate chip fixation hole, and chromatograph test strip is made of sample pad, nitrocellulose filter, bonding pad and absorption pad, and wherein nitrocellulose filter is located at centre, and sample pad and absorption pad are located at both ends；Bonding pad is between sample pad and nitrocellulose filter；Channel layer includes detection zone through-hole, liquid pool to be measured, prepare liquid channel, cleaning liquid pool, cleaning solution channel, main channel, sample inlet pool, waste liquid pool, venting channels and channel chip fixation hole.The utility model provide a kind of clean hygiene, detection precisely, the multiple determination centrifugal type microfludic test paper chip of high degree of automation.
Description
Technical field
The utility model relates to micro-fluidic test paper chip detection technique field, more particularly to a kind of multi objective inspection
Survey centrifugal type microfludic test paper chip.
Background technique
Micro-fluidic chip is the emerging technology that chemistry or biological sample are operated and detected on chip.It is micro-
Fluidic chip can be integrated into the operating units such as the preparation of sample, reaction, separation and detection one several square centimeters of chip
On, by the manipulation and control to fluid in microchannel network, it is automatically performed analytic process.Since microfluidic chip technology has
Sample volume is small, integrated level is high, Yi Shixian automation control and the characteristics of high throughput analysis, so that being given birth on micro-fluidic chip
It is more convenient, quick, low in cost to change the more conventional analysis sample pre-treatments of operation.
Chromatograph test strip has been widely used in the fields such as clinical diagnosis, food safety, environment measuring.Detection method packet
Coloration, fluorescence, Magnetic testi etc. are included, depending on the marker in bonding pad, and if quantitative detection, such as fluorescence is needed to be measured
Cleaning solution is added dropwise in liquid after reaction, and removal detection zone is not associated with marker, reduces interference.Operator is needed in routine operation
Be added prepare liquid reaction wait for a period of time after, be manually added cleaning solution, then again with visually or easy detection device into
Row result is read, and the degree of automation is poor, influences detection efficiency.
Therefore, how to provide a kind of clean hygiene, detection precisely, the centrifugal miniflow of multiple determination of high degree of automation
Control the problem of test paper chip is those skilled in the art's urgent need to resolve.
Utility model content
In view of this, the present invention provides a kind of multiple determination centrifugal type microfludic test paper chips, will be micro-fluidic
Chip meets multi objective while detecting in conjunction with chromatograph test strip, liquid portion in the chip mostly after reaction, than independent test strips
It is much cleaner；Liquid enters next stage by centrifugation, and guarantees that different time enters different liquids by different centrifugal rotational speeds, uses
Centrifugation detecting instrument is transferred to complete follow-up work, after waiting time and progress after adding prepare liquid, cleaning solution in family
Continuous manual operation, improves the degree of automation.
To achieve the goals above, the utility model adopts the following technical solution:
A kind of multiple determination centrifugal type microfludic test paper chip, comprising: cover layer, channel layer and backplane level, wherein institute
It states cover layer and the backplane level respectively corresponds and is arranged at the top and bottom of the channel layer, and match with channel layer sealing
It closes；
The cover layer is provided with visual window, prepare liquid sample holes, cleaning solution sample holes, venthole and cover board chip and fixes
Hole；
The backplane level is provided with chromatograph test strip and bottom plate chip fixation hole, wherein the chromatograph test strip is by sample
Pad, nitrocellulose filter, bonding pad and absorption pad composition, wherein the nitrocellulose filter be located at centre, the sample pad and
The absorption pad is located at both ends；The bonding pad is between the sample pad and the nitrocellulose filter；The nitre
Detection line and nature controlling line are provided on acid cellulose film；
The channel layer is provided with detection zone through-hole, liquid pool to be measured, prepare liquid channel, cleaning liquid pool, cleaning solution channel, master
Channel, sample inlet pool, waste liquid pool, venting channels and channel chip fixation hole；Wherein, the prepare liquid channel is connected to the prepare liquid
Pond and the main channel, and the liquid pool to be measured is connected to the prepare liquid sample holes；Described in the cleaning solution channel connection
Liquid pool and the main channel are cleaned, and the cleaning liquid pool is connected to the cleaning solution sample holes；The main channel with it is described
Sample inlet pool connection；The venting channels are connected to the main channel and the venthole；The detection zone through-hole and the visual window
It is correspondingly arranged with the chromatograph test strip；The sample inlet pool is connected to the backplane level, liquid and the layer in the sample inlet pool
Analyse test strips contact；
The liquid pool to be measured, the prepare liquid channel, the cleaning liquid pool, the cleaning solution channel, the main channel, institute
State waste liquid pool, the venting channels are separated with the backplane level.
Preferably, in a kind of above-mentioned multiple determination centrifugal type microfludic test paper chip, the chromatograph test strip is parallel
Several are provided with, and each chromatograph test strip matching is provided with the corresponding sample inlet pool, the detection zone through-hole
With the visual window；The nitrocellulose of the chromatograph test strip and the detection zone through-hole and the visual window is corresponding sets
It sets, the anti-of detection line and nature controlling line on the nitrocellulose filter can be observed by the visual window and the detection zone through-hole
Answer state；The sample pad of the chromatograph test strip is correspondingly arranged with the sample inlet pool, enables liquid in the sample inlet pool
It flows in the sample pad, the bonding pad, the nitrocellulose filter, the absorption pad is stepped by chromatography effect, into
Row series reaction.
Preferably, in a kind of above-mentioned multiple determination centrifugal type microfludic test paper chip, the visual window is open type
Through-hole.
Preferably, it in a kind of above-mentioned multiple determination centrifugal type microfludic test paper chip, is provided in the bonding pad
Marker reacts the prepare liquid with marker.
Preferably, in a kind of above-mentioned multiple determination centrifugal type microfludic test paper chip, the shape in the cleaning solution channel
Shape includes but are not limited to waveform, and waveform channel can increase passage length, can also be fold-line-shaped or zigzag.
Preferably, in a kind of above-mentioned multiple determination centrifugal type microfludic test paper chip, the shape of the venting channels
Waveform is included but are not limited to, waveform channel can increase passage length, can also be fold-line-shaped or zigzag.
Preferably, in a kind of above-mentioned multiple determination centrifugal type microfludic test paper chip, the cover board chip fixation hole
Identical and coincidence setting, forms and runs through a kind of multi objective with the channel chip fixation hole, the fixed hole shape of bottom plate chip
Detect the chip fixation hole of centrifugal type microfludic test paper chip, a kind of centrifugal miniflow of multiple determination described in centrifugal process
Control test paper chip is fixed on centrifugation detector by the chip fixation hole, a kind of multiple determination centrifugal type microfludic
Test paper chip is centrifuged by center of circle rotation of the chip fixation hole.
Preferably, in a kind of above-mentioned multiple determination centrifugal type microfludic test paper chip, the cleaning liquid pool and described
Liquid pool to be measured is respectively positioned on the main channel close to the side of the channel chip fixation hole, in centrifugal process, the cleaning solution
Liquid can be in the case where being centrifuged power drive respectively from the cleaning solution channel and the prepare liquid channel in pond and the liquid pool to be measured
It flow in the main channel.
Preferably, described in the channel layer in a kind of above-mentioned multiple determination centrifugal type microfludic test paper chip
Detection zone through-hole, the cleaning liquid pool, the cleaning solution channel, the liquid pool to be measured, the venting channels, the prepare liquid are logical
Road, the main channel, the waste liquid pool, the sample inlet pool are by closely to being far successively arranged in channel chip fixation hole side；
When the slow-speed of revolution is centrifuged, a kind of multiple determination centrifugal type microfludic test paper chip passes through the chip fixation hole
It is fixed in the rotary shaft of the centrifugation detector, makes a kind of multiple determination centrifugal type microfludic test paper chip with described
Chip fixation hole is that center of circle rotation is centrifuged, and liquid flow process is as follows:
(1) slow-speed of revolution is centrifuged, and prepare liquid enters the main channel by the prepare liquid channel, and is full of all sample introductions
Pond, surplus liquid enter the waste liquid pool；
(2) static, prepare liquid contacts the sample pad of the chromatograph test strip, steps into the knot by chromatography effect
Pad, the nitrocellulose filter, the absorption pad are closed, series reaction is carried out；
(3) high speed centrifugation, cleaning solution are full of the sample inlet pool, extra liquid via the cleaning solution channel, the main channel
Body enters the waste liquid pool；
(4) static, cleaning solution contact the sample pad of the chromatograph test strip acted on by chromatography enter the bonding pad,
Remnants on the nitrocellulose filter are not associated with reagent and removed, avoid result by the nitrocellulose filter, the absorption pad
Interference when reading.
Preferably, in a kind of above-mentioned multiple determination centrifugal type microfludic test paper chip, a kind of multiple determination
The driving force of liquid is centrifugal force in centrifugal type microfludic test paper chip.
It can be seen via above technical scheme that compared with prior art, the utility model, which discloses, provides a kind of multi objective
Detection centrifugal type microfludic test paper chip meets multi objective while detecting by micro-fluidic chip in conjunction with chromatograph test strip, improves
Working efficiency, liquid portion in the chip mostly, more much cleaner than independent test strips after reaction；Liquid enters next stage by centrifugation,
And guarantee that different time enters different liquids by different centrifugal rotational speeds, user after adding prepare liquid, cleaning solution, transfer to from
Heart detecting instrument completes follow-up work, without the waiting time and carries out subsequent manual operation, improves the degree of automation.
Detailed description of the invention
In order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment
Or attached drawing needed to be used in the description of the prior art is briefly described, it should be apparent that, the accompanying drawings in the following description is only
It is the embodiments of the present invention, for those of ordinary skill in the art, without creative efforts, also
Other attached drawings can be obtained according to the attached drawing of offer.
Fig. 1 attached drawing is the three-dimensional explosive view of the utility model；
Fig. 2 attached drawing is channel layer Facad structure figure；
Fig. 3 attached drawing is chromatograph test strip structural schematic diagram.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work
Every other embodiment obtained, fall within the protection scope of the utility model.
The utility model embodiment discloses a kind of multiple determination centrifugal type microfludic test paper chip, by micro-fluidic chip
In conjunction with chromatograph test strip, meet multi objective while detecting, improves working efficiency, liquid portion in the chip mostly after reaction, than
Independent test strips are much cleaner；Liquid enters next stage by centrifugation, and guarantees that different time enters by different centrifugal rotational speeds
Different liquids, user transfer to centrifugation detecting instrument to complete follow-up work, without waiting after adding prepare liquid, cleaning solution
Time simultaneously carries out subsequent manual operation, improves the degree of automation.
In conjunction with attached drawing 1-3, the utility model discloses a kind of multiple determination centrifugal type microfludic test paper chips, comprising:
Cover layer 1, channel layer 2 and backplane level 3, wherein cover layer 1 and backplane level 3 respectively correspond the top and bottom that channel layer 2 is arranged in
Portion, and seal and cooperate with channel layer 2；
Cover layer 1 is provided with visual window 11, prepare liquid sample holes 12, cleaning solution sample holes 13, venthole 14 and cover board core
Piece fixation hole 15；
Backplane level 3 is provided with chromatograph test strip 31 and bottom plate chip fixation hole 32, and wherein chromatograph test strip 31 is by sample pad
33, nitrocellulose filter 34, bonding pad 27 and absorption pad 35 form, and nitrocellulose filter 34 is located at centre, sample pad 33 and suction
It receives pad 35 and is located at both ends；Bonding pad 27 is between sample pad 33 and nitrocellulose filter 34；On nitrocellulose filter 34
It is provided with detection line and nature controlling line；
It is logical that channel layer 2 is provided with detection zone through-hole 21, liquid pool to be measured 22, prepare liquid channel 23, cleaning liquid pool 24, cleaning solution
Road 25, main channel 26, sample inlet pool 29, waste liquid pool 210, venting channels 211 and channel chip fixation hole 212；Wherein, prepare liquid is logical
Road 23 is connected to liquid pool 22 to be measured and main channel 26, and liquid pool to be measured 22 is connected to prepare liquid sample holes 12；Cleaning solution channel 25
Connection cleaning liquid pool 24 and main channel 26, and clean liquid pool 24 and be connected to cleaning solution sample holes 13；Main channel 26 and sample inlet pool
29 connections；Venting channels 211 are connected to main channel 26 and venthole 14；Detection zone through-hole 21 and visual window 11 and chromatograph test strip 31
It is correspondingly arranged；Sample inlet pool 29 is connected to backplane level 3, and liquid is contacted with chromatograph test strip 31 in sample inlet pool 29；
It is liquid pool 22 to be measured, prepare liquid channel 23, cleaning liquid pool 24, cleaning solution channel 25, main channel 26, waste liquid pool 210, logical
Gas channel 211 is separated with backplane level 3.
In order to further optimize the above technical scheme, chromatograph test strip 31 is arranged in parallel with several, and each chromatography
The matching of test strips 31 is provided with corresponding sample inlet pool 29, detection zone through-hole 21 and visual window 11；The nitric acid of chromatograph test strip 31 is fine
It ties up plain film 34 to be correspondingly arranged with detection zone through-hole 21 and visual window 11, nitre can be observed by visual window 11 and detection zone through-hole 21
The reactiveness of detection line and nature controlling line on acid cellulose film 34；The sample pad 33 of chromatograph test strip 31 is corresponding with sample inlet pool 29 to be set
It sets, liquid in sample inlet pool 29 is enable to flow in sample pad 33, bonding pad 27, nitrocellulose filter are stepped by chromatography effect
34, absorption pad 35 carry out series reaction.
In order to further optimize the above technical scheme, visual window 11 is open type through-hole.
In order to further optimize the above technical scheme, it is provided with marker in bonding pad 27, keeps prepare liquid anti-with marker
It answers.
In order to further optimize the above technical scheme, the shape in cleaning solution channel 25 includes but are not limited to waveform, wave
Shape wave channel can increase passage length.
In order to further optimize the above technical scheme, the shape of venting channels 211 includes but are not limited to waveform, wave
Shape channel can increase passage length.
In order to further optimize the above technical scheme, cover board chip fixation hole 15 and channel chip fixation hole 212, bottom plate core
32 shape of piece fixation hole is identical and is overlapped setting, forms the chip for running through a kind of multiple determination centrifugal type microfludic test paper chip
Fixation hole, a kind of multiple determination centrifugal type microfludic test paper chip is fixed on centrifugation by chip fixation hole in centrifugal process
On detector, a kind of multiple determination centrifugal type microfludic test paper chip is centrifuged by center of circle rotation of chip fixation hole.
In order to further optimize the above technical scheme, it cleans liquid pool 24 and liquid pool to be measured 22 is respectively positioned on main channel 26 close to logical
The side of road chip fixation hole 212, in centrifugal process, cleaning liquid in liquid pool 24 and liquid pool to be measured 22 can drive in centrifugal force
It is flow in main channel 26 from cleaning solution channel 25 and prepare liquid channel 23 respectively under dynamic.
In order to further optimize the above technical scheme, in channel layer 2, detection zone through-hole 21, cleaning liquid pool 24, cleaning solution
Channel 25, liquid pool to be measured 22, venting channels 211, prepare liquid channel 23, main channel 26, waste liquid pool 210, sample inlet pool 29 are by closely extremely
Far successively it is arranged in 212 side of channel chip fixation hole；
When the slow-speed of revolution is centrifuged, a kind of multiple determination centrifugal type microfludic test paper chip by chip fixation hole be fixed on from
In the rotary shaft of heart detector, rotate a kind of multiple determination centrifugal type microfludic test paper chip with the chip fixation hole center of circle
It is centrifuged, liquid flow process is as follows:
(1) slow-speed of revolution is centrifuged, and prepare liquid enters main channel 26 by prepare liquid channel 23, and is full of sample inlet pool 29, extra liquid
Body enters waste liquid pool 210；
(2) static, prepare liquid contact chromatograph test strip 31 sample pad 33, by chromatography effect step into bonding pad 27,
Nitrocellulose filter 34, absorption pad 35 carry out series reaction；
(3) high speed centrifugation, cleaning solution are full of sample inlet pool 29 via cleaning solution channel 25, main channel 26, and surplus liquid enters
Waste liquid pool 210；
(4) static, the sample pad 33 that cleaning solution contacts chromatograph test strip 31 enters bonding pad 27, nitric acid fibre by chromatography effect
Plain film 34, absorption pad 35 are tieed up, the remnants on nitrocellulose filter 34 are not associated with reagent and are removed, it is dry when result being avoided to read
It disturbs.
In order to further optimize the above technical scheme, liquid in a kind of multiple determination centrifugal type microfludic test paper chip
Driving force is centrifugal force.
Each embodiment in this specification is described in a progressive manner, the highlights of each of the examples are with other
The difference of embodiment, the same or similar parts in each embodiment may refer to each other.For device disclosed in embodiment
For, since it is corresponded to the methods disclosed in the examples, so being described relatively simple, related place is said referring to method part
It is bright.
The foregoing description of the disclosed embodiments can be realized professional and technical personnel in the field or using originally practical new
Type.Various modifications to these embodiments will be readily apparent to those skilled in the art, and determine herein
The General Principle of justice can be realized in other embodiments without departing from the spirit or scope of the present utility model.Cause
This, the present invention will not be limited to the embodiments shown herein, and is to fit to and principles disclosed herein
The widest scope consistent with features of novelty.
Claims (10)
1. a kind of multiple determination centrifugal type microfludic test paper chip characterized by comprising cover layer, channel layer and bottom plate
Layer, wherein the cover layer and the backplane level respectively correspond be arranged at the top and bottom of the channel layer, and with the channel
Layer sealing cooperation；
The cover layer is provided with visual window, prepare liquid sample holes, cleaning solution sample holes, venthole and cover board chip fixation hole；
The backplane level is provided with chromatograph test strip and bottom plate chip fixation hole, wherein the chromatograph test strip is by sample pad, nitre
Acid cellulose film, bonding pad and absorption pad composition, wherein the nitrocellulose filter is located at centre, the sample pad and the suction
It receives pad and is located at both ends；The bonding pad is between the sample pad and the nitrocellulose filter；The cellulose nitrate
Detection line and nature controlling line are provided on plain film；
The channel layer is provided with detection zone through-hole, liquid pool to be measured, prepare liquid channel, cleaning liquid pool, cleaning solution channel, leads and lead to
Road, sample inlet pool, waste liquid pool, venting channels and channel chip fixation hole；Wherein, the prepare liquid channel is connected to the liquid pool to be measured
With the main channel, and the liquid pool to be measured is connected to the prepare liquid sample holes；The cleaning solution channel connection is described clear
Washing lotion pond and the main channel, and the cleaning liquid pool is connected to the cleaning solution sample holes；The main channel and it is described into
The connection of sample pond；The venting channels are connected to the main channel and the venthole；The detection zone through-hole and the visual window and
The chromatograph test strip is correspondingly arranged；The sample inlet pool is connected to the backplane level, liquid and the chromatography in the sample inlet pool
Test strips contact；
It is the liquid pool to be measured, the prepare liquid channel, the cleaning liquid pool, the cleaning solution channel, the main channel, described useless
Liquid pool, the venting channels are separated with the backplane level.
2. a kind of multiple determination centrifugal type microfludic test paper chip according to claim 1, which is characterized in that the layer
Analysis test strips are arranged in parallel with several, and each chromatograph test strip matching is provided with the corresponding sample inlet pool, institute
State detection zone through-hole and the visual window；The nitrocellulose filter of the chromatograph test strip and the detection zone through-hole and institute
Visual window is stated to be correspondingly arranged；The sample pad of the chromatograph test strip is correspondingly arranged with the sample inlet pool.
3. a kind of multiple determination centrifugal type microfludic test paper chip according to claim 2, which is characterized in that it is described can
Form is open type through-hole.
4. a kind of multiple determination centrifugal type microfludic test paper chip according to claim 2, which is characterized in that the knot
It closes in pad and is provided with marker.
5. a kind of multiple determination centrifugal type microfludic test paper chip according to claim 1, which is characterized in that described clear
The shape of washing liquid channel includes but are not limited to waveform.
6. a kind of multiple determination centrifugal type microfludic test paper chip according to claim 1, which is characterized in that described logical
The shape in gas channel includes but are not limited to waveform.
7. a kind of multiple determination centrifugal type microfludic test paper chip according to claim 1, which is characterized in that the lid
Board chip fixation hole is identical as the channel chip fixation hole, the fixed hole shape of bottom plate chip and is overlapped setting, is formed and runs through institute
State a kind of chip fixation hole of multiple determination centrifugal type microfludic test paper chip.
8. a kind of multiple determination centrifugal type microfludic test paper chip according to claim 1, which is characterized in that described clear
Washing lotion pond and the liquid pool to be measured are respectively positioned on the main channel close to the side of the channel chip fixation hole.
9. a kind of multiple determination centrifugal type microfludic test paper chip according to claim 1, which is characterized in that described
In channel layer, the detection zone through-hole, the cleaning liquid pool, the cleaning solution channel, the liquid pool to be measured, the ventilation are logical
Road, the prepare liquid channel, the main channel, the waste liquid pool, the sample inlet pool are by closely to being far successively arranged in the channel
Chip fixation hole side.
10. a kind of multiple determination centrifugal type microfludic test paper chip according to claim 1, which is characterized in that described
The driving force of liquid is centrifugal force in a kind of multiple determination centrifugal type microfludic test paper chip.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920324323.4U CN209680127U (en) | 2019-03-14 | 2019-03-14 | A kind of multiple determination centrifugal type microfludic test paper chip |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920324323.4U CN209680127U (en) | 2019-03-14 | 2019-03-14 | A kind of multiple determination centrifugal type microfludic test paper chip |
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| CN209680127U true CN209680127U (en) | 2019-11-26 |
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| CN201920324323.4U Active CN209680127U (en) | 2019-03-14 | 2019-03-14 | A kind of multiple determination centrifugal type microfludic test paper chip |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113325170A (en) * | 2021-06-16 | 2021-08-31 | 博奥生物集团有限公司 | Test paper chip device, micro-fluidic chip and lateral flow test paper strip |
| DE102022106693B3 (en) | 2022-03-22 | 2023-03-16 | Dermagnostix GmbH | Centrifugal Microfluidic Biochip |
-
2019
- 2019-03-14 CN CN201920324323.4U patent/CN209680127U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113325170A (en) * | 2021-06-16 | 2021-08-31 | 博奥生物集团有限公司 | Test paper chip device, micro-fluidic chip and lateral flow test paper strip |
| DE102022106693B3 (en) | 2022-03-22 | 2023-03-16 | Dermagnostix GmbH | Centrifugal Microfluidic Biochip |
| WO2023179810A1 (en) | 2022-03-22 | 2023-09-28 | Dermagnostix GmbH | Centrifugal microfluidic biochip |
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