CN108709880A - Reusable high throughput SERS micro-fluidic chips and its application - Google Patents
Reusable high throughput SERS micro-fluidic chips and its application Download PDFInfo
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- CN108709880A CN108709880A CN201810524022.6A CN201810524022A CN108709880A CN 108709880 A CN108709880 A CN 108709880A CN 201810524022 A CN201810524022 A CN 201810524022A CN 108709880 A CN108709880 A CN 108709880A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
- G01N21/658—Raman scattering enhancement Raman, e.g. surface plasmons
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
Abstract
The invention discloses a kind of reusable high throughput SERS micro-fluidic chips, include the reaction chip being cascaded by micro-fluidic pipeline and detection chip；The reaction chip is used to carry out hybrid reaction for the fluid sample containing heavy metal ion and DNA enzymatic reagent；Reactant after the detection chip is used to react reaction chip is detected.It is the content for being applied to traces of harmful metal ion in detection water environment the present invention also provides the application of aforementioned reusable high throughput SERS micro-fluidic chips.The SERS micro-fluidic chips that the present invention designs, can be used with repetitive cycling, and simple process and low cost is efficient, can be mass.The present invention can disposably detect multiple data, have quickly, efficiently, high-throughput the characteristics of detecting.
Description
Technical field
The present invention relates to a kind of detection devices of traces of harmful metal ion in water environment, and in particular to one kind being based on miniflow
Traces of harmful metal ion detection chip and its application associated with control technology and surface enhanced Raman technique.
Background technology
Surface enhanced Raman scattering (SERS) technology is a kind of novel Analytical Methods of Trace, it can be obtained from molecular level
The information that gene and chemical bond and microenvironment influence sample structure, and its finger-print is obtained in real time, information content is very big, removes
Have the characteristics that high sensitivity, high specificity, it is unmarked, not damaged and can real-time detection, will not also be by media such as solution
Interference, is widely used in the fields such as biochemistry detection.Micro-fluidic chip is a kind of applied to micro-full analytical system field
Core technology, because its size is small, at low cost, reagent consumption is small, rapidly and efficiently, it is easy of integration and automation the advantages that, biology, change
There is huge application potential in the fields such as, medicine, are one of the directions of the following analysis and detection development, therefore, by SERS skills
It is a very promising research topic that art is combined with micro-fluidic chip.
Currently, the common method that SERS technologies are combined with micro-fluidic chip, is exactly first to prepare SERS substrates, then
The substrate is transferred on micro-fluidic operating position.This two step operates the cost for virtually increasing time and process, in order to
Realize that detection, SERS substrates will be generally built in inside microfluidic channel, above-mentioned two-step method can also give subsequent bonding packaging work
Sequence brings many difficulties.The thing more limited to, a usual micro-fluidic device may only place a piece of SERS substrates, by a large amount of
After time process makes, only detection is primary, is just discarded, and time and value cost are all very expensive.
To solve the above-mentioned problems, the enrichment method quilt based on colloidal particle such as electrodynamics, optical tweezer method, nano-channel method
It is widely used, however, due to the very easy adherency of colloidal particle, reunion in liquid environment, there are detection efficiencies for these technologies
Low, the problems such as signal strength is weak so that the SERS activity of the micro-fluidic chip with Raman substrate is low, many application scenarios all
Use demand is not achieved at all.Therefore, a kind of that there is the active micro-fluidic chips of higher SERS and recyclable reuse, height
Sensitive, low cost, the preparation method of the SERS micro-fluidic chips of fast high-flux is particularly important.
Invention content
In view of the deficiencies of the prior art, the present invention provides a kind of reusable high throughput SERS micro-fluidic chips and
It is applied.
The present invention is connected using reaction chip and detection chip, when solution to be measured and reaction reagent (DNA enzymatic) pass through respectively
Micro-fluidic DNA enzymatic has occurred in long and narrow U-shaped channel and cuts reaction for the two when being pumped into reaction chip, includes that label Raman is visited
The reaction products such as the residual DNA base sequence of needle molecule are pumped by micro-fluidic in the sample cell of detection chip together, with
SERS substrate effects, which, which is modified with, to be incubated with one section of single stranded DNA of residual DNA base sequence complementary, the two
Change after a certain period of time, two sections of single stranded DNAs are annealed into double-strand, cause the Raman microprobe molecule of end mark close to SERS substrate tables
Face generates Raman signal, to indirect determination harmful metal ion content.In addition, by washes clean repeatedly, and core will be detected
Piece is opened, and replaces multiple SERS substrates of the inside, which can be used with repetitive cycling, due to that can place multiple SERS bases simultaneously
Bottom disposably detects multiple data, therefore the chip has quickly, and efficiently, high throughput detection, reusable feature have
Good application prospect.
The invention is realized in this way：
A kind of reusable high throughput SERS micro-fluidic chips, include be cascaded by micro-fluidic pipeline it is anti-
Answer chip and detection chip；
The reaction chip is used to carry out hybrid reaction for the fluid sample containing heavy metal ion and DNA enzymatic reagent；
Reactant after the detection chip is used to react reaction chip is detected.
Further scheme is：
The detection chip includes at least one SERS substrates, and SERS substrates are replaceable.
Further scheme is：
The reaction chip and detection chip include substrate and upper and lower cover plate, and substrate and upper and lower cover plate are bonded by magnet
Connection.
Further scheme is：
The substrate of the reaction chip is equipped with the microchannel for sample introduction, the multiple bendings being connected with microchannel
U-shaped hybrid reaction channel；The detection chip is equipped with microchannel and the sample cell for placing SERS substrates.
Further scheme is：
The micro-fluidic pipeline uses the hard PVC or PPR pipe of acid-alkali-corrosive-resisting.
Further scheme is：
The substrate material is dimethyl silicone polymer, and the material of upper cover plate and lower cover slip is makrolon；Substrate is placed
Among three, upper and lower cover plate is by small magnet by three's tight bond at four angles.
Further scheme is：
The quantity in the U-shaped hybrid reaction channel of the bending of the reaction chip is no less than 3, and the dimension width in channel
It is 50~400 microns；Depth dimensions are 10~100 microns, and the substrate thickness of reaction chip is 50~1000 microns, upper cover plate and
Lower cover chip size is 25 × 55 millimeters, and thickness is 1~6 millimeter；
Multiple parallel sample cells for being equipped with SERS substrates are provided in detection chip, it is micro-fluidic logical in detection chip
Road is arranged in parallel, while the channel is connected to one end of liquid inlet and liquid outlet；The dimension width in channel is 100~1000 micro-
Rice；Depth dimensions are 10~500 microns；Sample cell length × width × height is 4 × 5 × 1 millimeter；Intermediate substrate layer thickness is 50~
1500 microns；Upper cover plate and lower cover chip size are 25 × 55 millimeters, and thickness is 1~6 millimeter.
Further scheme is：
The quantity in the U-shaped hybrid reaction channel of the bending of the reaction chip is 3, and the dimension width in channel is
200 microns；Depth dimensions are 50 microns, and the substrate thickness of reaction chip is 500 microns, upper cover plate and lower cover chip size be 25 ×
55 millimeters, thickness is 3 millimeters；
Setting is there are three the parallel sample cell for being equipped with SERS substrates in detection chip, micro-fluidic logical in detection chip
Road is arranged in parallel, while the channel is connected to one end of liquid inlet and liquid outlet；The dimension width in channel is 500 microns；Depth
Size is 300 microns；Sample cell length × width × height is 4 × 5 × 1 millimeter；Intermediate substrate layer thickness is 1200 microns；Upper cover plate
It it is 25 × 55 millimeters with lower cover chip size, thickness is 3 millimeters.
It is to be applied to detection the present invention also provides the application of aforementioned reusable high throughput SERS micro-fluidic chips
The content of traces of harmful metal ion in water environment, specific detection method are as follows：
Solution to be measured and reaction reagent are pumped into reaction chip by micro-fluidic respectively, sent out in U-shaped hybrid reaction channel
Raw DNA enzymatic cuts reaction, includes the reaction product of the residual DNA base sequence of label Raman microprobe molecule together by micro-fluidic
It is pumped into the sample cell of detection chip, with SERS substrate effects, which is modified with can be with residual DNA base sequence
One section of complementary single stranded DNA is arranged, the two is hatched after a certain period of time, and two sections of single stranded DNAs are annealed into double-strand, lead to the drawing of end mark
Graceful probe molecule generates Raman signal close to SERS substrate surfaces, to complete to measure harmful metal ion content.
Further scheme is：
The dust technology washing 2 for being 0.3% by mass percent by reusable high throughput SERS micro-fluidic chips
~10min, preferably 5min；Then 2~10min, preferably 5min are washed with the pH PBS buffer solution for being 7.4；And it replaces
SERS substrates, you can to reuse.
The beneficial effects of the present invention are：
(1) the SERS micro-fluidic chips that the present invention designs, are opened by washes clean repeatedly, and by detection chip, are replaced
Multiple SERS substrates of the inside, the chip can be used with repetitive cycling, and breaching current SERS micro-fluidic chips can not repeat to make
Limitation, simple process and low cost is efficient, can be mass.
(2) the SERS micro-fluidic chips that the present invention designs, can place multiple SERS substrates, disposably detect multiple numbers simultaneously
According to, the low limitation of current SERS micro-fluidic chips detection flux is breached, therefore the SERS micro-fluidic chips have quickly, efficiently,
The characteristics of high throughput detection.
(3) method for the detection heavy metal ion that the present invention designs, first choice are the trace uranyl ion in detection water sample
(UO2 2+), mercury ion is also can detect, thorium ion etc., versatility is good, there is very strong actual application value.
Description of the drawings
Fig. 1 is the structural schematic diagram of SERS micro-fluidic chips of the present invention；
Fig. 2 is the use flow diagram of SERS micro-fluidic chips of the present invention；
Fig. 3 is SERS of the embodiment of the present invention one using the uranyl ion solution of SERS micro-fluidic chips detection various concentration
Detection figure；
Fig. 4 is to recycle SERS micro-fluidic chips detection uranyl ion in the embodiment of the present invention two after flushing
SERS detection figures；
Fig. 5 be in the embodiment of the present invention two after flushing recycle SERS micro-fluidic chip high throughputs detection uranyl from
The SERS relative standard deviation block diagrams of son；
Fig. 6 is that the SERS detection figures of SERS micro-fluidic chips detection thorium ion are utilized in the embodiment of the present invention three；
Fig. 7 is that the SERS detection figures of SERS micro-fluidic chips detection mercury ion are utilized in the embodiment of the present invention four；
Fig. 8 is that the SERS detection figures of SERS micro-fluidic chips detection lead ion are utilized in the embodiment of the present invention four；
Specific implementation mode
The reusable high throughput SERS micro-fluidic chips of the present invention are specifically described below in conjunction with the accompanying drawings.
As shown in Fig. 1, a kind of reusable high throughput SERS micro-fluidic chips, including pass through micro-fluidic pipeline 5
The reaction chip 3 and detection chip 9 being cascaded；Setting is there are two sample inlets 1 are reacted on reaction chip, with two samples
The U-shaped hybrid reaction channel 2 of the connected multiple bendings of entrance；U-shaped hybrid reaction channel also with react sample outlet 4 be connected, instead
Sample outlet 4 is answered to be connected with detection sample inlets 6 by micro-fluidic pipeline 5, detection sample inlets 6 are parallel with three by pipeline
The sample cell 8 for being equipped with SERS substrates be connected, there are one SERS substrates 7 for setting in each sample cell 8, and sample cell also passes through pipeline
It is connected with detection sample outlet 10.
As shown in Fig. 2, the use flow diagram of SERS micro-fluidic chips of the present invention, metal wait for measured ion and DNA enzymatic
Sample respectively enters reaction chip reaction, is detected subsequently into detection chip.In attached drawing 2,1200/1800 is the spectrogram position of Raman
It moves, cycle refers to the Raman peaks obtained after reuse 1,2,3 times, and every time using 3 data of test, cycle 3 times is exactly to have altogether
Using 4 times, 12 data.
Technical scheme of the present invention is described in further detail with several more specific embodiments below.
Embodiment one
A kind of application of reusable high throughput SERS micro-fluidic chips, includes the following steps：
1) SERS substrates are modified；
There will be the single stranded DNA of number of base complementation to take out defrosting, centrifugation time 60s, rotating speed with double-stranded DNA
6000r.p.；50 μM of solution are configured to, drops in the clean SERS substrates that above-mentioned brand-new is got ready, places the substrate above moistening
In environment, it is 4 DEG C to keep temperature, is incubated 8h.Then the SERS substrate pure water rinsings that DNA will have been modified, are immersed in 6- sulfydryls-
A period of time in the aqueous solution of 1- ethyl alcohol (MCH), by its self assembly in substrate surface, with reduce DNA material surface non-spy
Opposite sex absorption, and prevent testing molecule from directly being acted on metal material.It is rinsed with MCH treated substrate deionized waters dry
Only, nitrogen dries up.
2) a kind of substrate with surface enhanced Raman scattering effect modified is selected to be placed into SERS micro-fluidic chips
Detection chip window in, such as Fig. 1, shown in Fig. 2, in Fig. 1,1 is the female Luer of reaction chip import, and 2 be the U of reaction chip
Type microfluidic channel, 3 be the makrolon cover board of reaction chip, and 4 female Luers exported for reaction chip, 5 be connection reaction
The pipeline of chip and detection chip, 6 be the female Luer of detection chip import, 7 be arranged in parallel for detection chip it is micro-fluidic logical
Road, 8 can place the detection window of SERS substrates for detection chip, be altogether 3；9 be the makrolon cover board of detection chip, and 10 are
The female Luer of detection chip outlet.DNA enzymatic shown in various concentration uranyl ion to be measured and table 1 is pumped by micropump together
In reaction chip, micropump is stopped, allowing after the two reaction 10min, continues to be pumped into detection chip, pump is stopped, reaction response
After a period of time, Raman detection is done, detection result is as shown in Fig. 3 Raman figures.
Embodiment two
A kind of application of reusable high throughput SERS micro-fluidic chips, includes the following steps：
1) such as one step 1) of the embodiment modification SERS substrates；
2) such as one step 2) of the embodiment progress SERS detections, and choose one in the different location of each SERS substrates
Point carries out Raman test, respectively three substrates in test chip.
3) dust technology for being 0.3% with mass percent continuously washs micro-fluidic chip 5min, and it is 7.4 then to use pH
After PBS buffer solutions wash micro-fluidic chip 5min, it is pumped into containing a concentration of 10-10The solution and DNA enzymatic of M uranyl ions are distinguished
It is pumped into reaction chip from the channel at both ends, micropump is stopped, being allowed after the two reaction 10min, continue to be pumped into detection chip, it will
Pump stop, reaction response for a period of time after, do Raman detection.A point is chosen in the different location of each SERS substrates to be drawn
Three substrates in test chip are distinguished in graceful test.
Then, above-mentioned rinsing step is repeated, after micro-fluidic chip is rinsed well, is passed through uranyl ion solution and DNA enzymatic
SERS detections are done after reaction again, repeat above step three times, can obtain recycling the SERS of SERS micro-fluidic chips three times
Detection figure (Fig. 4), and the disposable relative standard deviation block diagram (Fig. 5) for obtaining 12 SERS raman spectrums calculating and obtaining.
Embodiment three
A kind of application of reusable high throughput SERS micro-fluidic chips, includes the following steps：
1) SERS substrates are modified；
There will be the single stranded DNA of number of base complementation to take out defrosting, centrifugation time 60s, rotating speed with double-stranded DNA
6000r.p.；50 μM of solution are configured to, drops in the clean SERS substrates that above-mentioned brand-new is got ready, places the substrate above moistening
In environment, it is 4 DEG C to keep temperature, is incubated 8h.Then the SERS substrate pure water rinsings that DNA will have been modified, are immersed in 6- sulfydryls-
A period of time in the aqueous solution of 1- ethyl alcohol (MCH), by its self assembly in substrate surface, with reduce DNA material surface non-spy
Opposite sex absorption, and prevent testing molecule from directly being acted on metal material.It is rinsed with MCH treated substrate deionized waters dry
Only, nitrogen dries up.
2) a kind of substrate with surface enhanced Raman scattering effect modified is selected to be placed into SERS micro-fluidic chips
Detection chip window in, such as Fig. 1, shown in 2, be pumped into instead by micropump by thorium ion to be measured and together with DNA enzymatic shown in table 1
It answers in chip, micropump is stopped, allowing after the two reaction 10min, continue to be pumped into detection chip, pump is stopped, reaction response one
After the section time, Raman detection is done, effect is as shown in Figure 6.
Example IV
A kind of application of reusable high throughput SERS micro-fluidic chips, includes the following steps：
1) SERS substrates are modified；
There will be the single stranded DNA of number of base complementation to take out defrosting, centrifugation time 60s, rotating speed with double-stranded DNA
6000r.p.；50 μM of solution are configured to, drops in the clean SERS substrates that above-mentioned brand-new is got ready, places the substrate above moistening
In environment, it is 4 DEG C to keep temperature, is incubated 8h.Then the SERS substrate pure water rinsings that DNA will have been modified, are immersed in 6- sulfydryls-
A period of time in the aqueous solution of 1- ethyl alcohol (MCH), by its self assembly in substrate surface, with reduce DNA material surface non-spy
Opposite sex absorption, and prevent testing molecule from directly being acted on metal material.It is rinsed with MCH treated substrate deionized waters dry
Only, nitrogen dries up.
2) a kind of substrate with surface enhanced Raman scattering effect modified is selected to be placed into SERS micro-fluidic chips
Detection chip window in, such as Fig. 1, shown in 2, be pumped into instead by micropump by mercury ion to be measured and together with DNA enzymatic shown in table 1
It answers in chip, micropump is stopped, allowing after the two reaction 10min, continue to be pumped into detection chip, pump is stopped, reaction response one
After the section time, Raman detection is done, effect is as shown in Figure 7.
Embodiment five
A kind of application of reusable high throughput SERS micro-fluidic chips, includes the following steps：
1) SERS substrates are modified；
There will be the single stranded DNA of number of base complementation to take out defrosting, centrifugation time 60s, rotating speed with double-stranded DNA
6000r.p.；50 μM of solution are configured to, drops in the clean SERS substrates that above-mentioned brand-new is got ready, places the substrate above moistening
In environment, it is 4 DEG C to keep temperature, is incubated 8h.Then the SERS substrate pure water rinsings that DNA will have been modified, are immersed in 6- sulfydryls-
A period of time in the aqueous solution of 1- ethyl alcohol (MCH), by its self assembly in substrate surface, with reduce DNA material surface non-spy
Opposite sex absorption, and prevent testing molecule from directly being acted on metal material.It is rinsed with MCH treated substrate deionized waters dry
Only, nitrogen dries up.
2) a kind of substrate with surface enhanced Raman scattering effect modified is selected to be placed into SERS micro-fluidic chips
Detection chip window in, such as Fig. 1, shown in 2, be pumped into instead by micropump by lead ion to be measured and together with DNA enzymatic shown in table 1
It answers in chip, micropump is stopped, allowing after the two reaction 10min, continue to be pumped into detection chip, pump is stopped, when reacting one section
Between after, do Raman detection, effect is as shown in Figure 8.
Table 1. is the DNA sequence dna signal used in the embodiment of the present invention one to five.
Although reference be made herein to invention has been described for explanatory embodiment of the invention, and above-described embodiment is only this hair
Bright preferable embodiment, embodiment of the present invention are not limited by the above embodiments, it should be appreciated that people in the art
Member can be designed that a lot of other modification and implementations, these modifications and implementations will be fallen in principle disclosed in the present application
Within scope and spirit.
Claims (10)
1. a kind of reusable high throughput SERS micro-fluidic chips, it is characterised in that：
Include the reaction chip being cascaded by micro-fluidic pipeline and detection chip；
The reaction chip is used to carry out hybrid reaction for the fluid sample containing heavy metal ion and DNA enzymatic reagent；
Reactant after the detection chip is used to react reaction chip is detected.
2. reusable high throughput SERS micro-fluidic chips according to claim 1, it is characterised in that：
The detection chip includes at least one SERS substrates, and SERS substrates are replaceable.
3. reusable high throughput SERS micro-fluidic chips according to claim 1, it is characterised in that：
The reaction chip and detection chip include substrate and upper and lower cover plate, and substrate and upper and lower cover plate are bonded by magnet to be connected
It connects.
4. reusable high throughput SERS micro-fluidic chips according to claim 2, it is characterised in that：
The substrate of the reaction chip is equipped with the microchannel for sample introduction, the U for the multiple bendings being connected with microchannel
Shape hybrid reaction channel；The detection chip is equipped with microchannel and the sample cell for placing SERS substrates.
5. reusable high throughput SERS micro-fluidic chips according to claim 1, it is characterised in that：
The micro-fluidic pipeline uses the hard PVC or PPR pipe of acid-alkali-corrosive-resisting.
6. reusable high throughput SERS micro-fluidic chips according to claim 3, it is characterised in that：
The substrate material is dimethyl silicone polymer, and the material of upper cover plate and lower cover slip is makrolon；Substrate is placed on three
Among person, upper and lower cover plate is by small magnet by three's tight bond at four angles.
7. reusable high throughput SERS micro-fluidic chips according to claim 4, it is characterised in that：
The quantity in the U-shaped hybrid reaction channel of the bending of the reaction chip is no less than 3, and the dimension width in channel is 50
~400 microns；Depth dimensions are 10~100 microns, and the substrate thickness of reaction chip is 50~1000 microns, upper cover plate and lower cover
Chip size is 25 × 55 millimeters, and thickness is 1~6 millimeter；
Multiple parallel sample cells for being equipped with SERS substrates are provided in detection chip, the micro-fluidic channel in detection chip is simultaneously
Townhouse arranges, while the channel is connected to one end of liquid inlet and liquid outlet；The dimension width in channel is 100~1000 microns；It is deep
It is 10~500 microns to spend size；Sample cell length × width × height is 4 × 5 × 1 millimeter；Intermediate substrate layer thickness is 50~1500
Micron；Upper cover plate and lower cover chip size are 25 × 55 millimeters, and thickness is 1~6 millimeter.
8. reusable high throughput SERS micro-fluidic chips according to claim 7, it is characterised in that：
The quantity in the U-shaped hybrid reaction channel of the bending of the reaction chip is 3, and the dimension width in channel is 200 micro-
Rice；Depth dimensions are 50 microns, and the substrate thickness of reaction chip is 500 microns, and upper cover plate and lower cover chip size are 25 × 55 millis
Rice, thickness are 3 millimeters；
Setting is there are three the parallel sample cell for being equipped with SERS substrates in detection chip, and the micro-fluidic channel in detection chip is simultaneously
Townhouse arranges, while the channel is connected to one end of liquid inlet and liquid outlet；The dimension width in channel is 500 microns；Depth dimensions
It is 300 microns；Sample cell length × width × height is 4 × 5 × 1 millimeter；Intermediate substrate layer thickness is 1200 microns；Upper cover plate is under
Cover plate size is 25 × 55 millimeters, and thickness is 3 millimeters.
9. a kind of application of reusable high throughput SERS micro-fluidic chips, it is characterised in that be to be applied to detection water environment
The content of middle traces of harmful metal ion, specific detection method are as follows：
Solution to be measured and reaction reagent are pumped into reaction chip by micro-fluidic respectively, DNA occurs in U-shaped hybrid reaction channel
Endonuclease reaction includes that the reaction product of the residual DNA base sequence of Raman microprobe molecule is marked to be pumped into together by micro-fluidic
Into the sample cell of detection chip, with SERS substrate effects, which is modified with can be mutual with residual DNA base sequence
The one section of single stranded DNA mended, the two are hatched after a certain period of time, and two sections of single stranded DNAs are annealed into double-strand, and the Raman of end mark is caused to be visited
Needle molecule generates Raman signal close to SERS substrate surfaces, to complete to measure harmful metal ion content.
10. the application of reusable high throughput SERS micro-fluidic chips according to claim 9, it is characterised in that：
By reusable high throughput SERS micro-fluidic chips by mass percent be 0.3% dust technology washing 2~
Then 10min washs 2~10min with the pH PBS buffer solution for being 7.4, and replaces SERS substrates, you can to reuse.
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| CN110208247A (en) * | 2019-06-24 | 2019-09-06 | 西安电子科技大学 | A kind of dynamic fluid n cell Surface enhanced Raman scattering detection device and method |
| CN110314716A (en) * | 2019-07-19 | 2019-10-11 | 武汉理工大学 | A kind of micro fluidic device |
| CN110346345A (en) * | 2019-06-04 | 2019-10-18 | 中国工程物理研究院化工材料研究所 | A kind of detection method of high quick DNA hydrogel to uranyl ion concentration |
| CN110487770A (en) * | 2019-09-22 | 2019-11-22 | 杭州未来已来科技有限公司 | One kind based on SERS- it is micro-fluidic associated with UO22+The rapid detection method of ion |
| CN112007702A (en) * | 2019-05-31 | 2020-12-01 | 天津大学青岛海洋技术研究院 | Micro-fluidic chip for detecting metal pollution of underground pipe network water body weight |
| CN115096871A (en) * | 2022-07-22 | 2022-09-23 | 香港科技大学深圳研究院 | Detection device applied to multichannel SERS micro-fluidic chip |
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| CN112007702A (en) * | 2019-05-31 | 2020-12-01 | 天津大学青岛海洋技术研究院 | Micro-fluidic chip for detecting metal pollution of underground pipe network water body weight |
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| CN110208247A (en) * | 2019-06-24 | 2019-09-06 | 西安电子科技大学 | A kind of dynamic fluid n cell Surface enhanced Raman scattering detection device and method |
| CN110314716A (en) * | 2019-07-19 | 2019-10-11 | 武汉理工大学 | A kind of micro fluidic device |
| CN110487770A (en) * | 2019-09-22 | 2019-11-22 | 杭州未来已来科技有限公司 | One kind based on SERS- it is micro-fluidic associated with UO22+The rapid detection method of ion |
| CN110487770B (en) * | 2019-09-22 | 2022-08-09 | 瑞莱谱(杭州)医疗科技有限公司 | UO based on SERS-microfluidic combination 22+ Rapid ion detection method |
| CN115096871A (en) * | 2022-07-22 | 2022-09-23 | 香港科技大学深圳研究院 | Detection device applied to multichannel SERS micro-fluidic chip |
| CN115096871B (en) * | 2022-07-22 | 2022-12-23 | 香港科技大学深圳研究院 | Detection device applied to multichannel SERS micro-fluidic chip |
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