CN110487770A - One kind based on SERS- it is micro-fluidic associated with UO22+The rapid detection method of ion - Google Patents
One kind based on SERS- it is micro-fluidic associated with UO22+The rapid detection method of ion Download PDFInfo
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- CN110487770A CN110487770A CN201910896162.0A CN201910896162A CN110487770A CN 110487770 A CN110487770 A CN 110487770A CN 201910896162 A CN201910896162 A CN 201910896162A CN 110487770 A CN110487770 A CN 110487770A
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- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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Abstract
The invention discloses one kind based on SERS- it is micro-fluidic associated with UO2 2+The rapid detection method of ion, belongs to UO2 2+Ion detection field, one kind based on SERS- it is micro-fluidic associated with UO2 2+The rapid detection method of ion, it may be implemented through the back-shaped setting from isocon, it may be implemented the pyrolysis product for being marked with RhB being divided into the parallel sample of three equal parts automatically, it does not need artificially to be sampled, it effectively prevents because the amount of sample is different, and causes the difference of detection data, on the one hand improve the accuracy of detection, on the other hand the time for having saved manual sampling, entire UO is accelerated2 2+The detection procedure of ion, while by parallel shift chain, it does not need to shift sample again, three SERS biochips can be detected to three parts of samples simultaneously, it can directly be detected back-shaped from isocon, save detection time, accelerate entire UO2 2+The detection speed and efficiency of ion.
Description
Technical field
The present invention relates to UO2 2+Ion detection field, more specifically to one kind based on SERS- it is micro-fluidic associated with
UO2 2+The rapid detection method of ion.
Background technique
In recent years, with the development of nuclear undertaking, being continuously increased for uranium consumption amount causes serious uranium pollution.Uranyl from
Son (UO2 2+) it is most stable of uranium chemical species in water environment, there is very high bioavilability, pole can be caused to human health
Big harm.Currently, detecting the common method of uranium in environment mainly has ICP-MS, x-ray fluorescence method etc., but all exist sensitive
Spend the problems such as not high, detection signal is weak, dependence large-scale instrument, it is difficult to realize the scene of nuclear emergency needs after nuclear accident occurs quickly
Analysis.
Surface enhanced Raman scattering (SERS) is a kind of novel trace characterization technique based on Raman scattering.SERS technology is not
The pretreatment process of complexity is needed, detection time-consuming is short, response is fast, easy to operate, and popularizing with miniaturization Raman spectrometer,
In conjunction with SERS substrate, detection work can be carried out in complicated site environment, be a kind of trace being suitably applied in the case of nuclear emergency
Measure the emerging technology of uranyl ion on-site test.However, existing SERS research be concentrated mainly on uranyl ion Adsorption law and
Speciation analysis, the report for carrying out quick, accurate, high-throughput on-site test to true environment sample are also very deficient.
Certain researcher team devises the double-stranded DNA for having specific recognition to UO22+ ion first, and the 5 of substrate chain
Position end is marked letter and calls the score sub- rhodamine B (RhB).By double-stranded DNA and UO to be measured2 2+Solion each leads into micro-fluidic chip
Pipeline in, DNA enzymatic cracking reaction carries out in micro-fluidic long and narrow U-shaped microchannel.After reaction, the substrate chain of double-stranded DNA exists
The site rA is broken, and the pyrolysis product for being marked with RhB is split into three parts of parallel samples, is carried out for three pieces SERS biochip
High-flux parallel test.SERS biochip is that had with the three-dimensional ZnO-Ag meso-porous nano piece that single stranded DNA (ssDNA) is modified
Sequence array, wherein ssDNA is the sequence complementary with crack DNA (csDNA) of 5 '-RhB label in reaction product.The two is annealed into
After double-strand, letter calls the score sub- RhB by close to three-dimensional ZnO-Ag composite substrate surface, generates Raman signal.Pass through Raman signal
Power, the concentration variation of indirect reaction UO22+.The sensor pair is obtained by establishing standard curve, and according to calculation formula
The detectable limit (LOD) of UO22+ is 3.71 × 10-15 M.This result clearly fulfils the requirement of Environmental Protection Agency (EPA)
(130 nM).Compared with other analysis methods of nearest 2 years reports, this is at present to the most sensitive square of UO22+ ion detection
Method.In addition, when UO22+ ion concentration only has the 0.005% of other 15 kinds of toxic metal ions concentration, this biosensor
Still the ability of excellent Selective recognition UO22+ ion can be shown.Then, author is using biosensor to five kinds of differences
Concentration (n=6) is simulated to be detected by the tap water and river water of UO22+ ionic soil.It is rinsed by buffer solution, by chip
It reuses 2 times, each concentration level is achieved with 6 parallel datas, shows its efficient detectability.Meanwhile five kinds of differences
The sample recovery rate of concentration (n=6) is that 95.2% ~ 106.3%, RSD value is 2.6% ~ 5.8%.In addition, entire DNA enzymatic cracking reaction and
High-flux parallel detection can carry out 3 times or more on reusable SERS- micro-fluidic biological sensor.
But three parts of parallel samples wherein are split into the pyrolysis product for being marked with RhB, for three pieces SERS biochip
During carrying out high-flux parallel test, when pyrolysis product shunts in U-shaped microchannel, it is difficult to accomplish equivalent, subtle difference
Can also there be certain influence for testing result, cause testing staff for UO2 2+The judgement of ion mistake, and into
During row high throughput detection, there is the process of transfer sample, extend detection time, detection speed is generated centainly
It influences.
Summary of the invention
1. technical problems to be solved
Aiming at the problems existing in the prior art, the purpose of the present invention is to provide one kind based on SERS- it is micro-fluidic associated with
UO2 2+The rapid detection method of ion, it may be implemented to may be implemented to be marked with RhB's by the back-shaped setting from isocon
Pyrolysis product is divided into the parallel sample of three equal parts automatically, does not need artificially to be sampled, effectively prevents the amount because of sample
Difference, and cause the difference of detection data, on the one hand improves the accuracy of detection, on the other hand saved manual sampling when
Between, accelerate entire UO2 2+The detection procedure of ion, while by parallel shift chain, it does not need to shift sample again, can incite somebody to action
Three SERS biochips are detected to three parts of samples simultaneously, can directly be detected back-shaped from isocon, are saved
About detection time, accelerates entire UO2 2+The detection speed and efficiency of ion.
2. technical solution
To solve the above problems, the present invention adopts the following technical scheme that.
One kind based on SERS- it is micro-fluidic associated with UO2 2+The rapid detection method of ion, comprising the following steps:
S1, the double-stranded DNA and UO to be measured that 5 ends of substrate chain are marked to RhB2 2+Solion each leads into micro-fluidic chip
Pipeline in;
S2, DNA enzymatic cracking reaction is carried out in the channel, after reaction, the substrate chain of double-stranded DNA is broken in the site rA, is formed
It is marked with the pyrolysis product of RhB;
S3, then in the channel by the back-shaped pyrolysis product that will be marked with RhB from isocon, automatic shunt is at three parts of equal part
Parallel sample;
S4, pass through three parallel shift chains later, reduce by three parts of parallel sample liquid levels, while converting the position of SERS biochip
It sets, immerses it in sample liquid level, so that three pieces SERS biochip carries out high-flux parallel test to three parts of parallel samples.
Further, the pipeline in the S1 is long and narrow U-shaped microchannel.
Further, between the U-shaped microchannel and back-shaped from being connected with conduit between isocon in turn and with electromagnetism
Air bag is removed in the transfer of valve, and the parallel shift chain is arranged back-shaped from isocon, removes air bag by conduit and transfer, can incite somebody to action
The pyrolysis product for being marked with RhB is transferred to back-shaped from isocon, and can be divided into automatically back-shaped from isocon
Three equal parts, by transfer go air bag can be effectively ensured be transferred to it is back-shaped from the pyrolysis product that the label of isocon has into
Enter air, effectively avoid forming bubble in sample, effectively avoids influence of the bubble to later period biochip test precision.
Further, it is described it is back-shaped from isocon include left sample U-tube, middle sample U-tube, right sample U-tube and liquid
Overflow pipe, and is connected with transfer pipe and the lateral pipe that overflows therebetween per adjacent, and the transverse direction overflows pipe below upper transfer pipe,
Three parallel shift chains are separately positioned in transfer pipe, when be marked with RhB pyrolysis product enter it is back-shaped from isocon
Afterwards, when liquid level reaches at laterally excessive pipe in left sample U-tube, can enter along laterally excessive pipe in sample U-tube, Zhi Houzai
Enter in right sample U-tube by laterally excessive pipe, after finally flowing into hydrorrhea pipe, can stop continuing to shift, due to laterally overflowing
The setting of pipe and hydrorrhea pipe, extra sample can flow into hydrorrhea pipe, so that left sample U-tube, middle sample U-tube and right sample
Liquid level is consistent in U-tube, concordant at laterally excessive pipe lower edge.
Further, the excessive nozzle for managing close hydrorrhea pipe of the transverse direction of the rightmost side goes out to be fixedly connected with water conservancy diversion pillar, institute
It states and is provided with half web plate inside hydrorrhea pipe, digging in the middle part of half web plate has concave groove, and bottom end is installed in the concave groove
There is the pressure sensor connecting with solenoid signal, water conservancy diversion pillar has the function of water conservancy diversion, and the cracking for flowing into hydrorrhea pipe produces
Object can drip along water conservancy diversion pillar and from the end of water conservancy diversion pillar, rather than along the inner wall of hydrorrhea pipe to flowing down, so that cracking
Product can generate certain impact force to pressure sensor under the effect of gravity, and pressure sensor feeds back information to solenoid valve,
Solenoid valve is closed, and is stopped lysate sample and is continued to shift.
Further, the water conservancy diversion pillar is obliquely installed down to the right, and water conservancy diversion post tips are located in the middle part of hydrorrhea pipe, so that
The pyrolysis product dripped from water conservancy diversion strut ends can accurately drip on pressure sensor, consequently facilitating pressure sensor signal
Reception, so as to effectively control the waste of pyrolysis product.
Further, described and pressure sensor is located at the 5-8cm immediately below water conservancy diversion strut ends, hypertelorism, in weight
The active force being ultimately applied on pressure sensor under power acceleration effect can be excessive, accelerates the damage of pressure sensor, distance
Too small, active force is too small, causes the recognition effect of pressure sensor may be poor, cannot accurately and timely transmit and close to solenoid valve
Information, cause lysate sample to be taken excessively, cause to waste.
Further, half web plate includes solid middle part and netted outer rim, and the solid middle part and netted outer rim are fixed
Connection, solid middle part is for installing carrying pressure sensor, and netted outer rim is for being discharged downwards the pyrolysis product of drippage.
Further, the parallel shift chain includes the arc rotating plate with rotation connection at the top of upper transfer pipe, the arc
Rotating plate upper end is fixedly connected with driving lever, and two lower ends of described arc rotating plate or so are to have lead, the lower end point of two leads
It is not fixedly connected with the loading plate for being equipped with SERS biochip and inverted round stage block, and the volume of inverted round stage block is greater than and is equipped with
The loading plate of SERS biochip, so that liquid level may remain in the pipe that laterally overflows hereinafter, simultaneously after loading plate and the displacement of inverted round stage block
And three SERS biochips can be detected to three parts of samples simultaneously by parallel shift chain, and do not need again
Sample is shifted, can directly be detected back-shaped from isocon, save detection time, accelerate entire UO2 2+Ion
Detect speed and efficiency.
Further, the S3 before proceeding, first stirs the driving lever, controls the inverted round stage block and is located at the pipe that laterally overflows
Lower section, the loading plate for being equipped with SERS biochip is located at laterally excessive Guan Shangfang, by inverted round stage block, so that carrying out S4
When, loading plate and inverted round stage block replace, so that the liquid level of three parallel samples is below the pipe that laterally overflows, so that being detected
When, from laterally excessive pipe convection current will not occur for sample, and then guarantee left sample U-tube, middle sample U-tube and right sample U-tube
Interior sample detection independently carries out.
3. beneficial effect
Compared with the prior art, the present invention has the advantages that
(1) pyrolysis product that this programme may be implemented to may be implemented to be marked with RhB by the back-shaped setting from isocon is automatic
It is divided into the parallel sample of three equal parts, does not need artificially to be sampled, is effectively prevented because the amount of sample is different, and causes to examine
The difference of measured data, on the one hand improves the accuracy of detection, has on the other hand saved the time of manual sampling, accelerates entire
UO2 2+The detection procedure of ion, at the same by parallel shift chain, it does not need to shift sample again, it can be by three SERS biology cores
Piece is detected to three parts of samples simultaneously, can directly be detected back-shaped from isocon, save detection time, added
Fast entire UO2 2+The detection speed and efficiency of ion.
(2) pipeline in S1 is long and narrow U-shaped microchannel.
(3) it is gone between U-shaped microchannel with the back-shaped transfer from conduit is connected between isocon in turn and with solenoid valve
Air bag, parallel shift chain are arranged back-shaped from isocon, remove air bag by conduit and transfer, can will be marked with splitting for RhB
Solution product is transferred to back-shaped from isocon, and three equal parts can be divided into automatically from isocon back-shaped, in
Turn to go air bag can be effectively ensured be transferred to it is back-shaped from the pyrolysis product that the label of isocon has into air, effectively keep away
Exempt to form bubble in sample, effectively avoids influence of the bubble to later period biochip test precision.
(4) it is back-shaped from isocon include left sample U-tube, middle sample U-tube, right sample U-tube and hydrorrhea pipe, and every phase
Adjacent is connected with therebetween transfer pipe and the pipe that laterally overflows, and the pipe that laterally overflows is located at below upper transfer pipe, three parallel shift chains
Be separately positioned in transfer pipe, when be marked with RhB pyrolysis product enter it is back-shaped from isocon after, when in left sample U-tube
When liquid level is reached at lateral excessive pipe, the right side can be entered using laterally excessive pipe later along in sample U-tube in laterally excessive pipe entrance
In sample U-tube, after finally flowing into hydrorrhea pipe, it can stop continuing to shift, it is more due to the setting of laterally overflow pipe and hydrorrhea pipe
Remaining sample can flow into hydrorrhea pipe, so that liquid level keeps one in left sample U-tube, middle sample U-tube and right sample U-tube
It causes, it is concordant at laterally excessive pipe lower edge.
(5) the excessive nozzle for managing close hydrorrhea pipe of the transverse direction of the rightmost side goes out to be fixedly connected with water conservancy diversion pillar, sets inside hydrorrhea pipe
It installs and is equipped with half web plate, digging in the middle part of half web plate has concave groove, and bottom end is equipped with the pressure connecting with solenoid signal in concave groove
Force snesor, water conservancy diversion pillar have the function of water conservancy diversion, and the pyrolysis product for flowing into hydrorrhea pipe can be along water conservancy diversion pillar and from leading
The end of stream pillar is dripped, rather than along the inner wall of hydrorrhea pipe to flowing down, so that pyrolysis product under the effect of gravity can be to pressure
Force snesor generates certain impact force, and pressure sensor feeds back information to solenoid valve, and solenoid valve is closed, and stops lysate sample
Continue shift.
(6) water conservancy diversion pillar is obliquely installed down to the right, and water conservancy diversion post tips are located in the middle part of hydrorrhea pipe, so that from water conservancy diversion pillar
The pyrolysis product of end drippage can accurately drip on pressure sensor, consequently facilitating the reception of pressure sensor signal, from
And it can effectively control the waste of pyrolysis product.
(7) and pressure sensor is located at the 5-8cm immediately below water conservancy diversion strut ends, and hypertelorism is made in acceleration of gravity
The active force being ultimately applied on pressure sensor under can be excessive, accelerates the damage of pressure sensor, apart from too small, active force
It is too small, cause the recognition effect of pressure sensor may be poor, the information that cannot accurately and timely close to solenoid valve transmitting causes
Lysate sample is taken excessively, causes to waste.
(8) half web plates include solid middle part and netted outer rim, and solid middle part is fixedly connected with netted outer rim, and solid middle part is used
Pressure sensor is carried in installation, netted outer rim is for being discharged downwards the pyrolysis product of drippage.
(9) parallel shift chain includes the arc rotating plate with rotation connection at the top of upper transfer pipe, and arc rotating plate upper end is fixed to be connected
It is connected to driving lever, two lower end of arc rotating plate or so is to have lead, and the lower end of two leads, which has been respectively fixedly connected with, is equipped with SERS
The loading plate and inverted round stage block of biochip, and the volume of inverted round stage block is greater than the loading plate for being equipped with SERS biochip, makes
Loading plate and inverted round stage block displacement after, liquid level may remain in laterally overflow pipe hereinafter, and by parallel shift chain can will
Three SERS biochips are detected to three parts of samples simultaneously, and do not need to shift sample again, can directly returned
Shape is detected from isocon, has saved detection time, accelerates the detection speed and efficiency of entire UO22+ ion.
(10) S3 before proceeding, first stirs driving lever, and control inverted round stage block is located at below laterally excessive pipe, and it is raw to be equipped with SERS
The loading plate of object chip is located at laterally excessive Guan Shangfang, by inverted round stage block, so that loading plate and inverted round stage block change when carrying out S4
Position, so that the liquid level of three parallel samples is below the pipe that laterally overflows, so that when being detected, sample will not be from laterally excessive pipe
Convection current occurs, and then guarantees that the sample detection in left sample U-tube, middle sample U-tube and right sample U-tube is independent progress
's.
Detailed description of the invention
Fig. 1 is main flow diagram of the invention;
Fig. 2 is the back-shaped structural schematic diagram from isocon of the invention;
Fig. 3 is the structural schematic diagram in Fig. 2 at A;
Fig. 4 is the structural schematic diagram in Fig. 2 at B;
Fig. 5 is the structural schematic diagram of part when the loading plate on parallel shift chain of the invention is located at sample liquid level or more;
Fig. 6 is the structural schematic diagram of part when the loading plate on parallel shift chain of the invention is located at sample liquid level or less.
Figure label explanation:
1 conduit, 21 left sample U-tubes, sample U-tube in 22,23 right sample U-tubes, 24 hydrorrhea pipes, transfer pipe, 3 transfers on 25
Remove air bag, 4 laterally excessive pipe, 5 water conservancy diversion pillars, 6 half web plates, 7 pressure sensors, 81 driving levers, 82 arc rotating plates, 9 leads, 10 roundings
Platform block.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention;Technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description;Obviously;Described embodiments are only a part of the embodiments of the present invention;Instead of all the embodiments, it is based on
Embodiment in the present invention;It is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment;It shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the instruction such as term " on ", "lower", "inner", "outside", " top/bottom end "
Orientation or positional relationship be based on the orientation or positional relationship shown in the drawings, be merely for convenience of description the present invention and simplification retouch
It states, rather than the device or element of indication or suggestion meaning must have a particular orientation, be constructed and operated in a specific orientation,
Therefore it is not considered as limiting the invention.In addition, term " first ", " second " are used for description purposes only, and cannot understand
For indication or suggestion relative importance.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " installation " " is set
Be equipped with ", " be arranged/connect ", " connection " etc., shall be understood in a broad sense, such as " connection ", may be a fixed connection, be also possible to removable
Connection is unloaded, or is integrally connected;It can be mechanical connection, be also possible to be electrically connected;It can be directly connected, it can also be in
Between medium be indirectly connected, can be the connection inside two elements.It for the ordinary skill in the art, can be specific
Situation understands the concrete meaning of above-mentioned term in the present invention.
Embodiment 1:
Referring to Fig. 1, it is a kind of based on SERS- it is micro-fluidic associated with UO2 2+The rapid detection method of ion, comprising the following steps:
S1, the double-stranded DNA and UO to be measured that 5 ends of substrate chain are marked to RhB2 2+Solion each leads into micro-fluidic chip
Pipeline in, pipeline be long and narrow U-shaped microchannel;
S2, DNA enzymatic cracking reaction is carried out in the channel, after reaction, the substrate chain of double-stranded DNA is broken in the site rA, is formed
It is marked with the pyrolysis product of RhB;
S3, then in the channel by the back-shaped pyrolysis product that will be marked with RhB from isocon, automatic shunt is at three parts of equal part
Parallel sample;
S4, pass through three parallel shift chains later, reduce by three parts of parallel sample liquid levels, while converting the position of SERS biochip
It sets, immerses it in sample liquid level, so that three pieces SERS biochip carries out high-flux parallel test to three parts of parallel samples.
Referring to Fig. 5, S3 is before proceeding, driving lever 81 is first stirred, control inverted round stage block 10 is located at laterally excessive 4 lower section of pipe,
The loading plate for being equipped with SERS biochip is located at laterally excessive 4 top of pipe, by inverted round stage block 10, so that holding when carrying out S4
Support plate and inverted round stage block 10 replace, so that the liquid level of three parallel samples is below laterally excessive pipe 4, so that when being detected,
From laterally excessive pipe 4 convection current will not occur for sample, and then guarantee left sample U-tube 21, middle sample U-tube 22 and right sample U-shaped
Sample detection in pipe 23 independently carries out, referring to Fig. 6, parallel shift chain includes being rotatablely connected at the top of upper transfer pipe 25
Arc rotating plate 82,82 upper end of arc rotating plate is fixedly connected with driving lever 81, and 82 or so two lower end of arc rotating plate is to have lead 9,
The lower end of two leads 9 is respectively fixedly connected with the loading plate for being equipped with SERS biochip and inverted round stage block 10, and inverted round stage
The volume of block 10 can stir driving lever 81 after obtaining three parallel samples greater than the loading plate for being equipped with SERS biochip
To opposite direction, so that loading plate is located at sample liquid level hereinafter, inverted round stage block 10 is located at sample liquid level or more, so that loading plate
After being shifted with inverted round stage block 10, sample liquid level may remain in laterally excessive pipe 4 hereinafter, and can will by parallel shift chain
Three SERS biochips are detected to three parts of samples simultaneously, and do not need to shift sample again, can directly returned
Shape is detected from isocon, has saved detection time, accelerates entire UO2 2+The detection speed and efficiency of ion.
Referring to Fig. 2, between U-shaped microchannel and back-shaped from being connected with conduit 1 between isocon in turn and with solenoid valve
Transfer remove air bag 3, parallel shift chain is arranged back-shaped from isocon, removes air bag 3 by conduit 1 and transfer, can will mark
It is back-shaped from isocon that note has the pyrolysis product of RhB to be transferred to, and can be divided into automatically three from isocon back-shaped
Equal portions go air bag 3 that can be effectively ensured to be transferred to and back-shaped enter from the pyrolysis product that the label of isocon has by transfer
Air effectively avoids forming bubble in sample, effectively avoids influence of the bubble to later period biochip test precision, it is back-shaped from
Isocon includes left sample U-tube 21, middle sample U-tube 22, right sample U-tube 23 and hydrorrhea pipe 24, and per adjacent the two
Between be connected with transfer pipe 25 and laterally excessive pipe 4, laterally excessive pipe 4 is located at upper 25 lower section of transfer pipe, three parallel shift chains point
Not She Zhi in upper transfer pipe 25, when be marked with RhB pyrolysis product enter it is back-shaped from isocon after, when left sample U-tube 21
When interior liquid level is reached at lateral excessive pipe 4, it can be managed later using laterally overflowing along in sample U-tube 22 in the lateral excessive entrance of pipe 4
4 enter in right sample U-tube 23, after finally flowing into hydrorrhea pipe 24, can stop continuing to shift, due to pipe 4 and the liquid of laterally overflowing
The setting of excessive pipe 24, extra sample can flow into hydrorrhea pipe 24, so that left sample U-tube 21, middle sample U-tube 22 and right sample
Liquid level is consistent in product U-tube 23, concordant at laterally excessive 4 lower edge of pipe.
Fig. 3-4 is please referred to, the excessive pipe 4 of the transverse direction of the rightmost side goes out to be fixedly connected with water conservancy diversion pillar 5 close to the nozzle of hydrorrhea pipe 24,
Half web plate 6 is provided with inside hydrorrhea pipe 24, digging in the middle part of half web plate 6 has concave groove, and bottom end is equipped with and electricity in concave groove
The pressure sensor 7 of magnet valve signal connection, water conservancy diversion pillar 5 have the function of water conservancy diversion, flow into the pyrolysis product of hydrorrhea pipe 24
It can drip along water conservancy diversion pillar 5 and from the end of water conservancy diversion pillar 5, rather than along the inner wall of hydrorrhea pipe 24 to flowing down, so that splitting
Solution product can generate certain impact force to pressure sensor 7 under the effect of gravity, and pressure sensor 7 feeds back information to electromagnetism
Valve, solenoid valve are closed, and are stopped lysate sample and are continued to shift, water conservancy diversion pillar 5 is obliquely installed down to the right, and 5 end of water conservancy diversion pillar
Positioned at 24 middle part of hydrorrhea pipe, the pyrolysis product dripped from 5 end of water conservancy diversion pillar is accurately dripped in pressure sensor 7
On, consequently facilitating the reception of 7 signal of pressure sensor, so as to effectively control the waste of pyrolysis product, and pressure sensor 7
5-8cm immediately below 5 end of water conservancy diversion pillar, hypertelorism are ultimately applied to pressure sensing under acceleration of gravity effect
Active force on device 7 can be excessive, accelerates the damage of pressure sensor 7, and apart from too small, active force is too small, leads to pressure sensor 7
Recognition effect may it is poor, cannot accurately and timely to solenoid valve transmitting close information, cause lysate sample to be taken excessively, make
It include solid middle part and netted outer rim at half web plate 6 of waste, solid middle part is fixedly connected with netted outer rim, and solid middle part is for pacifying
Dress carrying pressure sensor 7, netted outer rim is for being discharged downwards the pyrolysis product of drippage.
It may be implemented to may be implemented by the back-shaped setting from isocon to divide equally the pyrolysis product for being marked with RhB automatically
It at the parallel sample of three equal parts, does not need artificially to be sampled, effectively prevent because the amount of sample is different, and cause testing number
According to difference, on the one hand improve the accuracy of detection, on the other hand saved the time of manual sampling, accelerate entire UO2 2+
The detection procedure of ion, at the same by parallel shift chain, it does not need to shift sample again, it can be same by three SERS biochips
When pair detected with three parts of samples, can directly be detected back-shaped from isocon, save detection time, accelerated
Entire UO2 2+The detection speed and efficiency of ion.
It is described above;It is merely preferred embodiments of the present invention;But scope of protection of the present invention is not limited thereto;
Anyone skilled in the art is in the technical scope disclosed by the present invention;According to the technique and scheme of the present invention and its
It improves design and is subject to equivalent substitution or change;It should be covered by the scope of protection of the present invention.
Claims (10)
1. one kind based on SERS- it is micro-fluidic associated with UO2 2+The rapid detection method of ion, it is characterised in that: including following step
It is rapid:
S1, the double-stranded DNA and UO to be measured that 5 ends of substrate chain are marked to RhB2 2+Solion each leads into micro-fluidic chip
Pipeline in;
S2, DNA enzymatic cracking reaction is carried out in the channel, after reaction, the substrate chain of double-stranded DNA is broken in the site rA, is formed
It is marked with the pyrolysis product of RhB;
S3, then in the channel by the back-shaped pyrolysis product that will be marked with RhB from isocon, automatic shunt is at three parts of equal part
Parallel sample;
S4, pass through three parallel shift chains later, reduce by three parts of parallel sample liquid levels, while converting the position of SERS biochip
It sets, immerses it in sample liquid level, so that three pieces SERS biochip carries out high-flux parallel test to three parts of parallel samples.
2. it is according to claim 1 it is a kind of based on SERS- it is micro-fluidic associated with UO2 2+The rapid detection method of ion, it is special
Sign is: the pipeline in the S1 is long and narrow U-shaped microchannel.
3. it is according to claim 1 it is a kind of based on SERS- it is micro-fluidic associated with UO2 2+The rapid detection method of ion, it is special
Sign is: between the U-shaped microchannel and back-shaped from being connected with conduit (1) between isocon in turn and with the transfer of solenoid valve
It goes air bag (3), the parallel shift chain is arranged back-shaped from isocon.
4. it is according to claim 3 it is a kind of based on SERS- it is micro-fluidic associated with UO2 2+The rapid detection method of ion, it is special
Sign is: it is described it is back-shaped from isocon include left sample U-tube (21), middle sample U-tube (22), right sample U-tube (23) and
Hydrorrhea pipe (24), and it is connected with transfer pipe (25) therebetween and the pipe (4) that laterally overflows per adjacent, the excessive pipe (4) of the transverse direction
Below upper transfer pipe (25), three parallel shift chains are separately positioned on transfer pipe (25).
5. it is according to claim 1 it is a kind of based on SERS- it is micro-fluidic associated with UO2 2+The rapid detection method of ion, it is special
Sign is: the nozzle of the excessive pipe (4) of the transverse direction of the rightmost side close to hydrorrhea pipe (24) goes out to be fixedly connected with water conservancy diversion pillar (5), institute
It states and is provided with half web plate (6) inside hydrorrhea pipe (24), digging in the middle part of half web plate (6) has concave groove, the concave groove
Interior bottom end is equipped with the pressure sensor (7) connecting with solenoid signal.
6. it is according to claim 5 it is a kind of based on SERS- it is micro-fluidic associated with UO2 2+The rapid detection method of ion, it is special
Sign is: the water conservancy diversion pillar (5) is obliquely installed down to the right, and water conservancy diversion pillar (5) end is located in the middle part of hydrorrhea pipe (24).
7. it is according to claim 5 it is a kind of based on SERS- it is micro-fluidic associated with UO2 2+The rapid detection method of ion, it is special
Sign is: described and pressure sensor (7) is located at the 5-8cm immediately below water conservancy diversion pillar (5) end.
8. it is according to claim 1 it is a kind of based on SERS- it is micro-fluidic associated with UO2 2+The rapid detection method of ion, it is special
Sign is: half web plate (6) includes solid middle part and netted outer rim, and the solid middle part is fixedly connected with netted outer rim.
9. it is according to claim 1 it is a kind of based on SERS- it is micro-fluidic associated with UO2 2+The rapid detection method of ion, it is special
Sign is: the parallel shift chain includes the arc rotating plate (82) with rotation connection at the top of upper transfer pipe (25), and the arc turns
Plate (82) upper end is fixedly connected with driving lever (81), and two lower ends of arc rotating plate (82) left and right are to have lead (9), described in two
The lower end of lead (9) is respectively fixedly connected with the loading plate for being equipped with SERS biochip and inverted round stage block (10), and inverted round stage
The volume of block (10) is greater than the loading plate for being equipped with SERS biochip.
10. it is according to claim 8 it is a kind of based on SERS- it is micro-fluidic associated with UO2 2+The rapid detection method of ion,
Be characterized in that: the S3 before proceeding, first stirs the driving lever (81), controls the inverted round stage block (10) and is located at the pipe that laterally overflows
(4) lower section, the loading plate for being equipped with SERS biochip are located above the pipe (4) that laterally overflows.
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