CN103954736B - Real-time micro-fluidic chip biochemical detection device under water - Google Patents
Real-time micro-fluidic chip biochemical detection device under water Download PDFInfo
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Abstract
The invention discloses one real-time micro-fluidic chip biochemical detection device and detection method thereof under water, described device comprises micro-fluidic chip carrying platform, micro-fluidic chip, detection components, data handling component, underwater environment information acquisition assembly, automatically sampling component, underwater sealing cabin, data transfer components and data display component; Described micro-fluidic chip carrying platform, micro-fluidic chip, detection components, data handling component, underwater environment information acquisition assembly and automatic sampling component are all encapsulated in underwater sealing cabin.The present invention adopts micro-fluidic chip as detection platform, can sample preparation involved in the fields such as biological and chemical, biological with chemical reaction, be separated, the basic operation unit such as detection is integrated on the micro-fluidic chip of a piece several square centimeters, form network by microchannel, run through whole system with controlled fluid.Volume of the present invention is little, simple to operate, sample size is little, detection is quick, accurate, without the need to carrying out complex process to sample before detection.
Description
Technical field
The present invention relates to marine eco-environment detection technique, particularly one real-time micro-fluidic chip biochemical detection device and detection method thereof under water.
Background technology
Along with the continuous anxiety of land resources and the continuous deterioration of environment, exploitation ocean resources become the vital task of countries in the world marine cause with protecting the marine environment.Marine environment is the pacing items of sea life survival and development, and any change of marine environment all likely causes marine ecosystem to unify the change of living resources.At present, marine environmental pollution is on the rise, heavy metal, organism, nutritive salt and a large amount of radiomaterials etc. enter ocean, cause marine ecosystem to unify living resources and energy distribution state generation great variety, the disaster such as red tide, EI Nino, coast erosion, eustasy also occurs again and again.Therefore must develop actively marine eco-environment monitoring technology, the in time Succession Trend of understanding marine ecosystems.
Monitoring platform and the method for the current marine eco-environment mainly contain: laboratory measurement, the direct method of measurement and microscale experiment room method.
Laboratory measurement is a kind of method utilizing the equipment in laboratory to detect seawater sample under seawater sample being taken to the environment such as laboratory, the method parameter measurement is accurate, measurement parameter is more, but the impact of surrounding environment is vulnerable in transit at seawater sample, change the state that seawater sample is original, the reliability that its testing result is not so good as real-time online detecting instrument is under water high.
The direct method of measurement utilizes sensor technology directly to measure pollution parameters under water, the method have relatively simple for structure, can detect fast, the advantage such as power consumption is lower and easy to use.But due to the reason on sensor technology, sensor technology no matter its kind, measurement parameter, accuracy of measurement still rests in the conventional parameter measurement levels such as hydrometeorology, and the sensor that the water quality biological state of reflection marine environmental pollution degree and the physical-chemical parameters are measured needs development.
Microscale experiment room method is that analytical approach effective in current laboratory is moved under water, and development is similar to the instrument in underwater miniature laboratory, seawater is extracted in instrument and detects.The monitor etc. of the employing Flow Analysis Technique of the APP4004 type pressure balance type analyser of the infiltration type analyser developed as aquarium, Monterrey of U.S. gulf (MBARI), the development of German ME company, the pressure equilibrium NAS-2EN type analysis instrument of WIS company of Britain development, the development of Chelsea Instrument Ltd. of Britain, although these instruments can be monitored under water automatically, these apparatus structures are huge, complex structure, expensive, can not be integrated portable.
To sum up analyze, most of method of current marine environmental monitoring also exists can only be measured conventional parameters such as hydrometeorology and water quality, the problem that can not detect water quality biological state and the physical-chemical parameters.And equipment is huge, expensive price, time and effort consuming, unstable by the restriction of much external condition, identification of indicator.A kind of simple and convenient, real-time live water quality biological state and the physical-chemical parameters on-line checkingi the technology that simultaneously can detect the basic parameter such as the hydrology and water quality is the key issue being badly in need of in marine eco-environment detection method field solving under water.
Summary of the invention
For solving the problems referred to above that prior art exists, the present invention to design a kind ofly can realize on-the-spotly under water detecting in real time, equipment cost is cheap, workload is little and identification of indicator is stable micro-fluidic chip biochemical detection device and detection method thereof in real time under water.
To achieve these goals, technical scheme of the present invention is as follows: one is real-time micro-fluidic chip biochemical detection device under water, comprises micro-fluidic chip carrying platform, micro-fluidic chip, detection components, data handling component, underwater environment information acquisition assembly, automatically sampling component, underwater sealing cabin, data transfer components and data display component; Described micro-fluidic chip carrying platform is unglazed enclosed construction, and described micro-fluidic chip and detection components are fixed in micro-fluidic chip carrying platform; Described automatic sampling component is connected with micro-fluidic chip; Described detection components is connected with micro-fluidic chip, and described detection components is also connected with data handling component; Described data handling component is also connected with underwater environment information acquisition assembly and data transfer components respectively; Described data transfer components is also connected with data display component; Described micro-fluidic chip carrying platform, micro-fluidic chip, detection components, data handling component, underwater environment information acquisition assembly and automatic sampling component are all encapsulated in underwater sealing cabin;
Described micro-fluidic chip is made up of substrate and cover plate, described substrate is dimethyl silicone polymer or polymethylmethacrylate, described cover plate is glass sheet or polymethylmethacrylate, and the two is bonded together and forms liquid storage hole, waste liquid hole, microchannel and surveyed area;
Described detection components comprises photoelectricity testing part and electro-chemical detection device; Detection components signal output part is connected with data handling component;
Described data handling component comprises Weak Signal Detecting Circuit, power driving circuit, data acquisition circuit and microprocessor and interface circuit thereof;
Described automatic sampling component comprises operation valve, conduit A, conduit B, screen pack and operation valve driving circuit, the output terminal of described conduit A is connected with operation valve respectively with the input end of conduit B, the input end through-fall lower seal of conduit A out of my cabin shell is connected with seawater, and the output terminal of conduit B is connected with the liquid storage hole of micro-fluidic chip; Screen pack is provided with in conduit A;
Described underwater environment information acquisition assembly comprises temperature sensor, depth transducer, salinity sensor, alignment sensor and PH sensor, and described underwater environment information acquisition assembly is connected with data handling component.
A detection method for real-time micro-fluidic chip biochemical detection device, comprises the steps: under water
A, seawater object detection area is put in packaged underwater sealing cabin;
Seawater sample around pick-up unit is successively imported by conduit A and conduit B in the liquid storage hole A of micro-fluidic chip by B, automatically sampling component, and the sample in liquid storage hole flows to waste liquid hole along main channel through surveyed area;
C, when the sample in seawater is through the surveyed area of micro-fluidic chip, detection components is according to detecting the detection of being correlated with to sample of target difference, and testing result outputs to data handling component by detection components;
D, data handling component carry out signal extraction, signals collecting and signal processing operations to testing result;
The data display component that data after E, data handling component process are sent on ship or on the bank by data transfer components again shows relevant testing result.
Compared with prior art, the present invention has following beneficial effect:
1, the present invention adopts micro-fluidic chip as detection platform, can sample preparation involved in the fields such as biological and chemical, biology and chemical reaction, the basic operation units such as separation, detection are integrated on the micro-fluidic chip of a piece several square centimeters, network is formed by microchannel, whole system is run through with controlled fluid, in order to complete different biological or chemical courses of reaction, therefore detection platform volume is little, simple to operate, sample size is little, detection is quick, accurate, without the need to carrying out complex process to sample before detection.
2, detection components of the present invention carries out much information detection by photoelectricity testing part, electro-chemical detection device, temperature sensor, salinity sensor and PH sensor to marine environment, such as, in the seawater Size of phytoplankton and the detection etc. of ATP, and data handling component small volume, add micro-fluidic chip detection platform, this equipment is easy to carry, with low cost, overcome the shortcoming that at present automatic detection instrument is bulky under water.
3, present invention employs underwater sealing technology, this equipment can be worked long hours under water.Automatic sampling component of the present invention, can complete the seawater sample sampling in object detection area automatically.Therefore, the present invention can be transported to scene easily, puts into the scene that surveyed area under water carries out underwater sample and detects in real time.
4, the present invention contains data transmission and is data display component, can make the staff's Real Time Observation testing result on land or ship.
Accompanying drawing explanation
The present invention has 5, accompanying drawing, wherein:
Fig. 1 is real-time micro-fluidic chip biochemical detection device structural representation under water;
Fig. 2 is the wherein a kind of fluidic chip structural representation detecting marine environment;
Fig. 3 is automatic sampling component structural representation;
Fig. 4 is underwater information environment acquisition component structural representation;
Fig. 5 is the chlorophyll fluorescence and the RPS testing result figure that measure micro-algae.
In figure: 1, micro-fluidic chip, 2, detection components, 3, micro-fluidic chip carrying platform, 4, data handling component, 5, underwater environment information acquisition assembly, 6, automatic sampling component, 7, underwater sealing cabin, 8, data transfer components, 9, data display component, 10, microslide, 12, surveyed area, 13, main channel, 14, impedance pulse sensing upstream detection passage, 15, impedance pulse senses downstream sense channel, 16, operation valve, 17, conduit A, 18, conduit B, 19, screen pack, 20, temperature sensor, 21, depth transducer, 22, salinity sensor, 23, alignment sensor, 24, PH sensor, 25, waste liquid hole.
Embodiment
Below in conjunction with accompanying drawing and example, the invention will be further described.
Fig. 1 shows one-piece construction block diagram of the present invention, as seen from Figure 1, real-time micro-fluidic chip biochemical detection device under water, comprises micro-fluidic chip carrying platform 3, micro-fluidic chip 1, detection components 2, data handling component 4, underwater environment information acquisition assembly 5, automatically sampling component 6, underwater sealing cabin 7, data transfer components 8 and data display component 9; Described micro-fluidic chip carrying platform 3 is unglazed enclosed construction, and described micro-fluidic chip 1 and detection components 2 are fixed in micro-fluidic chip carrying platform 3; Described automatic sampling component 6 is connected with micro-fluidic chip 1; Described detection components 2 is connected with micro-fluidic chip 1, and described detection components 2 is also connected with data handling component 4; Described data handling component 4 is also connected with underwater environment information acquisition assembly 5 and data transfer components 8 respectively; Described data transfer components 8 is also connected with data display component 9; Described micro-fluidic chip carrying platform 3, micro-fluidic chip 1, detection components 2, data handling component 4, underwater environment information acquisition assembly 5, automatically sampling component 6 are encapsulated in underwater sealing cabin 7.
In Fig. 2, microfluidic chip structure is a kind of structure of micro-fluidic chip 1, comprise microslide 10, liquid storage hole A, waste liquid hole 25, liquid storage hole C, liquid storage hole D, main channel 13, surveyed area 12, impedance pulse sensing upstream detection passage 14 and impedance pulse senses downstream sense channel 15, liquid storage hole A is connected with main channel 13, waste liquid hole 25 is connected with main channel 13, liquid storage hole C is connected with impedance pulse sensing upstream detection passage 14, liquid storage hole D is connected 15 with impedance pulse senses downstream sense channel, impedance pulse sensing upstream detection passage 14 is connected with surveyed area 12 respectively with impedance pulse senses downstream sense channel 15.All insert platinum electrode in liquid storage hole A, waste liquid hole 25, liquid storage hole C and liquid storage hole D, the platinum electrode inserted in liquid storage hole A, waste liquid hole 25, liquid storage hole C and liquid storage hole D is connected with data handling component.
Described detection components 2 comprises photoelectricity testing part, electro-chemical detection device; The signal output part of detection components 2 is connected with data handling component 4.
Described data handling component 4 comprises Weak Signal Detecting Circuit, power driving circuit, data acquisition circuit and microprocessor and interface circuit composition thereof;
Described automatic sampling component 6 as shown in Figure 3, comprises operation valve 16, conduit A17, conduit B18 and screen pack 19 and forms.Operation valve and conduit A17 coordinate the seawater around collecting and detecting device, and seawater is also delivered in the relevant liquid storage hole of micro-fluidic chip 1 by conduit B18 under the control of a control valve through screen pack 19.
Described underwater environment information acquisition assembly 5 as shown in Figure 4, comprises temperature sensor 20, depth transducer 21, salinity sensor 22, alignment sensor 23 and PH sensor 24.Temperature sensor 20, depth transducer 21, salinity sensor 22, alignment sensor 23 and PH sensor 24 to transmit the temperature of the pick-up unit surrounding seawater collected with a certain frequency to data handling component 4, pick-up unit is positioned at the undersea degree of depth, the salinity of pick-up unit surrounding seawater and the geographic position of pick-up unit and seawater PH information.
Micro-fluidic chip carrying platform 3, micro-fluidic chip 1, detection components 2, data handling component 4, underwater environment information acquisition assembly 5 and automatic sampling component 6 is packaged with in described underwater sealing cabin 7.
Specific works step of the present invention is as follows:
Micro-fluidic chip 1 is put into micro-fluidic chip carrying platform 3, seawater object detection area is put in packaged underwater sealing cabin 7.By the automatic sampling component 6 of transmission assembly 8 opening device, detection components 2 and data handling component 4.Seawater sample around equipment is successively inputted by conduit A and conduit B in the liquid storage hole of micro-fluidic chip 1 by automatic sampling component 6, sample in liquid storage hole passes through along micro-fluidic chip 1 passage and detects detection zone, detection components 2 in underwater sealing cabin 7 is according to detecting the different detection of being correlated with to sample of target, and testing result outputs to data handling component 4 by detection components 2.Data handling component 4 pairs of testing results carry out the operations such as signal extraction, signals collecting and signal transacting.The data display component 9 that data after data handling component 4 processes are sent on the bank or on ship by data transfer components 8 again shows relevant testing result.
Claims (1)
1. a real-time micro-fluidic chip biochemical detection device under water, is characterized in that: comprise micro-fluidic chip carrying platform (3), micro-fluidic chip (1), detection components (2), data handling component (4), underwater environment information acquisition assembly (5), automatically sampling component (6), underwater sealing cabin (7), data transfer components (8) and data display component (9); Described micro-fluidic chip carrying platform (3) is unglazed enclosed construction, and described micro-fluidic chip (1) and detection components (2) are fixed in micro-fluidic chip carrying platform (3); Described automatic sampling component (6) is connected with micro-fluidic chip (1); Described detection components (2) is connected with micro-fluidic chip (1), and described detection components (2) is also connected with data handling component (4); Described data handling component (4) is also connected with underwater environment information acquisition assembly (5) and data transfer components (8) respectively; Described data transfer components (8) is also connected with data display component (9); Described micro-fluidic chip carrying platform (3), micro-fluidic chip (1), detection components (2), data handling component (4), underwater environment information acquisition assembly (5) and automatic sampling component (6) are all encapsulated in underwater sealing cabin (7);
Described micro-fluidic chip (1) is made up of substrate and cover plate, described substrate is dimethyl silicone polymer or polymethylmethacrylate, described cover plate is glass sheet or polymethylmethacrylate, and the two is bonded together and forms liquid storage hole, waste liquid hole, microchannel and surveyed area (12);
Described detection components (2) comprises photoelectricity testing part and electro-chemical detection device; Detection components (2) signal output part is connected with data handling component (4);
Described data handling component (4) comprises Weak Signal Detecting Circuit, power driving circuit, data acquisition circuit and microprocessor and interface circuit thereof;
Described automatic sampling component (6) comprises operation valve (16), conduit A (17), conduit B (18), screen pack (19) and operation valve (16) driving circuit, the output terminal of described conduit A is connected with operation valve (16) respectively with the input end of conduit B (18), input end through-fall lower seal cabin (7) shell of conduit A (17) is connected with seawater, and the output terminal of conduit B (18) is connected with the liquid storage hole of micro-fluidic chip (1); Screen pack (19) is provided with in conduit A (17);
Described underwater environment information acquisition assembly (5) comprises temperature sensor (20), depth transducer (21), salinity sensor (22), alignment sensor (23) and PH sensor (24), and described underwater environment information acquisition assembly (5) is connected with data handling component (4).
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| CN109269560A (en) * | 2018-09-30 | 2019-01-25 | 华侨大学 | A kind of underwater movable platform data collection and transfering system |
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| CN106931949A (en) * | 2015-12-31 | 2017-07-07 | 中国科学院沈阳自动化研究所 | A kind of electronic module system and method for subaqueous survey |
| CN105973823A (en) * | 2016-06-22 | 2016-09-28 | 苏州汶颢芯片科技有限公司 | UV-visible absorption detection system based on micro-fluidic chip and detection method thereof |
| CN106290515B (en) * | 2016-10-28 | 2019-06-14 | 国家海洋技术中心 | The self calibration ocean multi-parameter chemical sensor of micro-fluidic replaceable cavity body structure |
| CN106442920B (en) * | 2016-11-11 | 2018-08-31 | 大连海事大学 | A kind of ballast water for ship discharge real time monitoring apparatus and method |
| CN108614576A (en) * | 2018-04-12 | 2018-10-02 | 澳门培正中学 | A kind of control method and system of underwater detectoscope |
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