CN105973823A - UV-visible absorption detection system based on micro-fluidic chip and detection method thereof - Google Patents
UV-visible absorption detection system based on micro-fluidic chip and detection method thereof Download PDFInfo
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- PTFCDOFLOPIGGS-UHFFFAOYSA-N Zinc dication Chemical compound [Zn+2] PTFCDOFLOPIGGS-UHFFFAOYSA-N 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
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- 229910001385 heavy metal Inorganic materials 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 description 2
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 description 2
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- 238000001499 laser induced fluorescence spectroscopy Methods 0.000 description 1
- 229910052745 lead Inorganic materials 0.000 description 1
- 229960004194 lidocaine Drugs 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
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- 238000002834 transmittance Methods 0.000 description 1
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- FGQOOHJZONJGDT-UHFFFAOYSA-N vanillin Natural products COC1=CC(O)=CC(C=O)=C1 FGQOOHJZONJGDT-UHFFFAOYSA-N 0.000 description 1
- 235000012141 vanillin Nutrition 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N2021/3129—Determining multicomponents by multiwavelength light
- G01N2021/3133—Determining multicomponents by multiwavelength light with selection of wavelengths before the sample
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- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
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Abstract
The application discloses a UV-visible absorption detection system based on a micro-fluidic chip and a detection method thereof. The UV-visible absorption detection system comprises a chip substrate, a micro-channel formed on the chip substrate and a UV absorption detector integrated on the chip substrate, wherein the micro-channel comprises a detection channel, and rays generated by the UV absorption detector are parallel to the extending direction of the detection channel. A light source of the UV absorption detector emits in and out in the horizontal direction of the chip, namely, in the direction parallel to the channel of the chip, so that the problem of short absorption optical path is solved; a UV-visible absorption detection operation unit is simple, low in detection cost and strong in university; the UV-visible absorption detector is integrated into the micro-fluidic chip, so that detection convenience is realized, and the quantity of the consumed solution can reach several or dozens of micro-liters. Compared with a traditional UV-visible absorption detection system, the detection method provided by the invention is greatly lowered in sample consumption, and has significance on detection of ions, micromolecules and the like in the field of environmental water or food.
Description
Technical field
The application belongs to Microfluid based Lab on a chip detection field, particularly relates to a kind of detecting system that ultravioletvisible absorption device is integrated in micro-fluidic chip, and this chip may be used for environment or field of food intermediate ion or little Molecular Detection.
Background technology
Microfluid based Lab on a chip (Lab on Chip) or claim micro-total analysis system (
Micro Total Analysis System, or microTAS) refer to the sample involved in the fields such as biological and chemical to prepare, biological integrated with the basic operation unit such as chemical reaction, separation detection or be substantially integrated on the chip of a piece several square centimeters, in order to complete different biological or chemical courses of reaction, and a kind of technology that its product is analyzed.It is by analytical chemistry, micro electronmechanical processing (
MEMS), computer, electronics, material science are intersected with biology, medical science and engineering etc. and are realized chemical analysis detection and i.e. realize the overall miniaturization from sample pretreating to detection, automatization, integrated and this target of portability.To conventional chemistry or biology laboratory, detection is all an indispensable step, and the most also be unable to do without this basic process of detection in order to realize the Microfluid based Lab on a chip of conventional chemical or the various feature operation of biology laboratory.Usually occur in the micro structure of micron dimension size with Microfluid based Lab on a chip for various chemistry, biologically and separation etc. that platform is carried out, this has very big difference with traditional similar operations, to this end, the detector in micro-fluidic chip requires have advantage highly sensitive, that fast response time, volume are little.
Micro-fluidic chip detector is generally divided into fluorescence detector, electrochemical detector, mass detector etc..Wherein, laser-Induced Fluorescence Detection is highly sensitive, in being applicable to chip microchannel various materials detection, but a lot of materials do not have photoluminescent property, need fluorescent labeling, whether a lot of experiments change due to the character of the measured matter after can not guaranteeing labelling, the reliability of experimental result can be directly affected, application is restricted, mass detector assembles with micro-fluidic chip, its interface is difficult point, Comparatively speaking, ultravioletvisible absorption detection operating unit is simple, testing cost is low, highly versatile, the portable of detection can be realized if being integrated in micro-fluidic chip, Environmental Water or the heavy metal of field of food or little molecule etc. are detected and produces highly important meaning.
Ultravioletvisible absorption principle is langbobier law, absorbance is directly proportional to absorbing light path, chip enriched sample being tried out to normal size can meet detection demand, but the sample for low concentration then needs design and makes the chip of long light path, this adds increased the difficulty of processing of chip and increase the consumption of detection sample, it is seen that being difficulty with long light path detection being perpendicular to chip channel direction.It addition, the chip materials such as conventional glass, plastics all have bigger absorption to ultraviolet light, it is not suitable for ultraviolet detection, and the chip materials such as quartz, PDMS are little to uv absorption, are the ideal materials of uv absorption photometric detection.
Summary of the invention
It is an object of the invention to provide a kind of based on micro-fluidic chip ultravioletvisible absorption detecting system and detection method thereof, to overcome deficiency of the prior art.
For achieving the above object, the present invention provides following technical scheme:
The embodiment of the present application is open a kind of based on micro-fluidic chip ultravioletvisible absorption detecting system, the microchannel that including chip basal body, is formed in chip basal body and be integrated in the ultraviolet absorption detector in described chip basal body, described microchannel includes sense channel, and the light ray parallel that described ultraviolet absorption detector produces is in the bearing of trend of described sense channel.
Preferably, above-mentioned based in micro-fluidic chip ultravioletvisible absorption detecting system, described ultraviolet absorption detector includes light source, two sections of optical fiber, collimating lens and photomultiplier tube, described light source is through one section of optical fiber input, source parallel is injected one end of sense channel by collimated lens, and penetrate from the other end of sense channel, then project photomultiplier tube through another section of optical fiber.
Preferably, above-mentioned based in micro-fluidic chip ultravioletvisible absorption detecting system, described sense channel is linear type passage.
Preferably, above-mentioned based in micro-fluidic chip ultravioletvisible absorption detecting system, a length of more than the 0.5cm of described sense channel.
Preferably, above-mentioned based in micro-fluidic chip ultravioletvisible absorption detecting system, described light source, collimating lens and photomultiplier tube set gradually along the bearing of trend of described sense channel.
Preferably, above-mentioned based in micro-fluidic chip ultravioletvisible absorption detecting system, described microchannel includes sample feeding mouth and is communicated in the hybrid channel between sample feeding mouth and sense channel, is also disposed with buffer injection port and developer injection port between described sample feeding mouth and hybrid channel.
Preferably, above-mentioned based in micro-fluidic chip ultravioletvisible absorption detecting system, in described hybrid channel curve extension or hybrid channel, array distribution has multiple projection strengthening mixing.
Preferably, above-mentioned based in micro-fluidic chip ultravioletvisible absorption detecting system, the port of export of described sense channel and waste fluid channel connection, Z-shaped or U-shaped connection between the port of export of described sense channel, waste fluid channel and hybrid channel.
Preferably, above-mentioned based in micro-fluidic chip ultravioletvisible absorption detecting system, described chip basal body material is PMMA.
Accordingly, disclosed herein as well is a kind of detection method based on micro-fluidic chip ultravioletvisible absorption detecting system, being used for quantitatively detecting sample intermediate ion or little molecule content, it is in certain concentration range, is calculated according to Lambert-Beer's law Criterion curve.
Compared with prior art, it is an advantage of the current invention that:
1. in detector, fiber optic conduction is passed through in incidence and the outgoing of light source, it is achieved that parallel light is in sense channel direction;
2. absorb light path to be determined by the length of chip channel, solve the problem that in traditional microfluidic chip ultraviolet detection, light path is shorter;
3. ultravioletvisible absorption detector is integrated in micro-fluidic chip, it is achieved that detection portable;
4. ultravioletvisible absorption detection is simple to operate, testing cost is low, highly versatile;
5. the mixing of solution under present system achieves micro-meter scale, react, detect.
6. the amount of solution used by present system is up to several microlitres or tens microlitres, greatly reduces amount of samples.
7. present system can detect environment or Metal Ions in Foodstuffs or little molecule, and detection sensitivity is high.
Accompanying drawing explanation
In order to be illustrated more clearly that the embodiment of the present application or technical scheme of the prior art, the accompanying drawing used required in embodiment or description of the prior art will be briefly described below, apparently, accompanying drawing in describing below is only some embodiments described in the application, for those of ordinary skill in the art, on the premise of not paying creative work, it is also possible to obtain other accompanying drawing according to these accompanying drawings.
Fig. 1 show the structural representation of detecting system in the specific embodiment of the invention.
Detailed description of the invention
It is an object of the invention to provide a kind of micro-fluidic chip system with ultravioletvisible absorption detection function, this system is, under external motivating force effect, solution is injected micro-fluidic chip, operate with the chromogenic reaction that the micro-fluidic chip being machined with hybrid detection MCA realizes sample and developer for carrier, then, carrying out ultravioletvisible absorption long light path detection along sense channel horizontal direction, this system is capable of portable detection by quantitative sample intermediate ion or the content of little molecule.
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is described in detail, it is clear that described embodiment is only a part of embodiment of the present invention rather than whole embodiments.Based on the embodiment in the present invention, the every other embodiment that those of ordinary skill in the art are obtained on the premise of not making creative work, broadly fall into the scope of protection of the invention.
Shown in Fig. 1, based on micro-fluidic chip ultravioletvisible absorption detecting system, the microchannel including chip basal body 1, being formed in chip basal body and the ultraviolet absorption detector 2 being integrated in chip basal body, microchannel includes sense channel 3, and the light ray parallel that ultraviolet absorption detector 2 produces is in the bearing of trend of sense channel 3.
In this system, external motivating force is to be realized by micro-injection pump, constant pressure pump, Miniature diaphragm pump, peristaltic pump etc..
Ultraviolet absorption detector 2 includes light source 21, collimating lens 22 and photomultiplier tube 23, light source 21 is incident through optical fiber 24, source parallel is injected one end of sense channel 3 by collimated lens 22, and penetrates from the other end of sense channel 3, then projects photomultiplier tube 23 through optical fiber 25.
Preferably, sense channel 3 is linear type passage.A length of more than the 0.5cm of sense channel.Light source 21, collimating lens 22 and photomultiplier tube 23 set gradually along the bearing of trend of sense channel 3.
In this technical scheme, photomultiplier tube is connected with computer, carries out data process.Light source need to select according to the characteristic absorption wavelength of different detection materials.The mounting groove of detector module is processed in sense channel both sides, it addition, the detecting system of entirety needs lucifuge to process.
The center of incident illumination needs in same level line with photomultiplier tube, to obtain maximum signal value.
Microchannel includes sample feeding mouth 4 and the hybrid channel 5 being communicated between sample feeding mouth 4 and sense channel 3, is also disposed with buffer injection port 6 and developer injection port 7 between sample feeding mouth 4 and hybrid channel 5.
In this technical scheme, the quantity of injection port according to different application at least provided with 2.System is to promote sample introduction with external micro-injection pump, and sample flows through the microchannel of micro-fluidic chip, carries out with developer being sufficiently mixed, reacting and enter sense channel, detects in integrated ultravioletvisible absorption device part, and carries out quantitative analysis.
In hybrid channel 5 curve extension or hybrid channel 5, array distribution has multiple projection strengthening mixing.
In this technical scheme, hybrid channel purpose is to promote sample and developer to be sufficiently mixed, react, and is shaped as the curve extensions such as S type, Z-type or has the concaveconvex structure (internal diameter size is along bearing of trend alternate) strengthening mixing.
The port of export and a waste fluid channel 8 of sense channel 3 connect, Z-shaped or U-shaped connection between the port of export 51 of sense channel 3, waste fluid channel 8 and hybrid channel.
In this technical scheme, by Z-type and U-shaped shape, can increase absorption light path, additionally the end of sense channel is arranged by square, it is simple to the parallel entrance of the integrated and light of ultraviolet absorption detector, turnover.
Chip basal body 1 material is preferably the PMMA that light transmittance is higher.PDMS, quartz, silicon etc. can also be selected.
The present embodiment detecting system, the sample of detection by quantitative can include that the heavy metal ion (such as Cu, Pb, Cd, Zn) in the field such as environment and food, little molecule are (such as Cl2), food additive (such as vanillin, Pyrusussuriensis alkyd, cyclamate) etc..Its detection method refers in certain concentration range, is calculated according to Lambert-Beer's law Criterion curve.
In one embodiment, the system have been the test of such as zinc ion, the principle of zinc ion detection is zinc ion and 2-(5-Bromo-2-pyridylazo)-5-(lignocaine) phenol (5-Br-PADAP) reacting generating complex in Borax buffer medium, has absorption maximum at 556 nm.Now the corresponding light source of detector should be that the wavelength of the light launched is 556
About nm.
In the present embodiment, the detailed process of zinc ion detection is:
1. being tightly connected with chip injection port by micro-injection pump, take the sample containing zinc ion, developer 5-Br-PADAP and buffer, injected in chip injection port by three kinds of appropriate solution, the pH of solution needs to maintain 7.0 ~ 9.5.
2. carry out in hybrid channel being sufficiently mixed through the solution of chip injection port, chromogenic reaction.
3. the stream product through hybrid channel is by sense channel.
4. open light source and photomultiplier tube, make parallel light pass through sense channel, then utilize analysis software to carry out data record and analysis, draw testing result.
It should be noted that, in this article, term " includes ", " comprising " or its any other variant are intended to comprising of nonexcludability, so that include that the process of a series of key element, method, article or equipment not only include those key elements, but also include other key elements being not expressly set out, or also include the key element intrinsic for this process, method, article or equipment.
The above is only the detailed description of the invention of the application; it should be pointed out that, for those skilled in the art, on the premise of without departing from the application principle; can also make some improvements and modifications, these improvements and modifications also should be regarded as the protection domain of the application.
Claims (10)
1. one kind based on micro-fluidic chip ultravioletvisible absorption detecting system, it is characterized in that, the microchannel that including chip basal body, is formed in chip basal body and be integrated in the ultraviolet absorption detector in described chip basal body, described microchannel includes sense channel, and the light ray parallel that described ultraviolet absorption detector produces is in the bearing of trend of described sense channel.
The most according to claim 1 based on micro-fluidic chip ultravioletvisible absorption detecting system, it is characterized in that: described ultraviolet absorption detector includes light source, two sections of optical fiber, collimating lens and photomultiplier tube, described light source is through one section of optical fiber input, source parallel is injected one end of sense channel by collimated lens, and penetrate from the other end of sense channel, then project photomultiplier tube through another section of optical fiber.
It is the most according to claim 2 based on micro-fluidic chip ultravioletvisible absorption detecting system, it is characterised in that: described sense channel is linear type passage.
It is the most according to claim 3 based on micro-fluidic chip ultravioletvisible absorption detecting system, it is characterised in that: a length of more than the 0.5cm of described sense channel.
It is the most according to claim 3 based on micro-fluidic chip ultravioletvisible absorption detecting system, it is characterised in that: described light source, collimating lens and photomultiplier tube set gradually along the bearing of trend of described sense channel.
The most according to claim 1 based on micro-fluidic chip ultravioletvisible absorption detecting system, it is characterized in that: described microchannel includes sample feeding mouth and is communicated in the hybrid channel between sample feeding mouth and sense channel, is also disposed with buffer injection port and developer injection port between described sample feeding mouth and hybrid channel.
It is the most according to claim 6 based on micro-fluidic chip ultravioletvisible absorption detecting system, it is characterised in that: in described hybrid channel curve extension or hybrid channel, array distribution has multiple projection strengthening mixing.
The most according to claim 6 based on micro-fluidic chip ultravioletvisible absorption detecting system, it is characterized in that: the port of export of described sense channel and waste fluid channel connection, Z-shaped or U-shaped connection between the port of export of described sense channel, waste fluid channel and hybrid channel.
It is the most according to claim 1 based on micro-fluidic chip ultravioletvisible absorption detecting system, it is characterised in that: described chip basal body material is PMMA.
10. the arbitrary described detection method based on micro-fluidic chip ultravioletvisible absorption detecting system of claim 1 to 9, it is characterized in that, being used for quantitatively detecting sample intermediate ion or little molecule content, it is in certain concentration range, is calculated according to langbobier law Criterion curve.
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CN112858177A (en) * | 2019-11-26 | 2021-05-28 | 武汉理工大学 | Heavy metal ion on-line measuring chip based on micro-fluidic extraction technique |
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