CN206229378U - A kind of centrifugal routine urinalysis detection micro-fluidic chip - Google Patents
A kind of centrifugal routine urinalysis detection micro-fluidic chip Download PDFInfo
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- CN206229378U CN206229378U CN201620940803.XU CN201620940803U CN206229378U CN 206229378 U CN206229378 U CN 206229378U CN 201620940803 U CN201620940803 U CN 201620940803U CN 206229378 U CN206229378 U CN 206229378U
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Abstract
A kind of centrifugal routine urinalysis detection micro-fluidic chip, it is characterised in that：Described micro-fluidic chip includes chip basal body, center fixing hole, sample holes, liquid storage tank, main channel, interface channel, reaction detection pond, waste liquid pool, passage；Described chip basal body has three layers, including cover layer, center-aisle layer, backplane level；Described center fixed hole position is in chip basal body geometric center and through chip basal body；Described sample holes are located in cover layer with passage, and sample holes are connected with liquid storage tank, and passage is connected by interface channel with waste liquid pool；Described liquid storage tank is connected with main channel；Described reaction detection pond is connected by interface channel with main channel；Described waste liquid pool is connected by interface channel with last reaction detection pond.Micro-fluidic chip is detected using a kind of centrifugal routine urinalysis of the present utility model, multiple indexs can be detected simultaneously on a chip, and will not interfered between each Testing index, greatly facilitate detection process.
Description
Technical field
The utility model is related to micro-fluidic chip detection technique field, and more particularly to a kind of centrifugal routine urinalysis detection is micro-
Fluidic chip.
Background technology
Routine urianlysis is one of clinical three big routine inspections, is clinically a very important trial inspection.Urine
Diagnosis and observation of curative effect of the routine inspection to disease in the urological system, such as observation of inflammation, calculus, tumour, Post kidney transplantation；Its
The diagnosis of his systemic disease, such as diabetes, hepatitis, the diagnosis of acute pancreatitis；The poison of the monitoring of safe medication, such as sulfamido
Side reaction；The auxiliary diagnosis of occupational disease, such as lead, mercury heavy metal poisoning；Also have important to the assessment of human health status
Clinical meaning.
The quick detection of composition mainly uses dry chemical scrip method in current urine, commercialization ELISA test strip project from
8 to 14.Detection project include proportion, occult blood, leucocyte, nitrite, pH value, UBG, bilirubin, albumen
Matter, glucose, ketoboidies, ascorbic acid, urinary calcium, creatinine, uric acid, microalbumin etc..Test strips operation is more complicated, to collect
Urine, then urine is poured into test tube, by test strips insertion test tube, after soaking all test positions, test strips are taken out, blot
Unnecessary urine, is then placed in being detected in equipment, if adjacent detection in not dry the meeting contaminated equipment, and test strips of test strips suction
Color is easily interfered between block.Based on above-mentioned deficiency, market in urgent need one kind is portable, quick, accurate, easy to use, price
Cheap urine quick detection product, realizes having become the quick detection of urinary fractions one kind efficiently by micro-fluidic chip
Detection means, microfluidic chip technology can not only realize quantitative sample injection, and can guarantee that non-interference between Testing index,
It is simple to operate, it is adapted to basic unit and is used with family.
Utility model content
A kind of centrifugal routine urinalysis detection micro-fluidic chip, it is characterised in that：Described micro-fluidic chip includes chip base
Body, center fixing hole, sample holes, liquid storage tank, main channel, interface channel, reaction detection pond, waste liquid pool, passage；Described core
Sheet matrix has three layers, including cover layer, center-aisle layer, backplane level；Described center fixed hole position is in chip basal body geometry
The heart and through chip basal body；Described sample holes are located in cover layer with passage, and described sample holes are connected with liquid storage tank,
Described passage is connected by interface channel with waste liquid pool；Described liquid storage tank is connected with main channel；Described reaction detection
Pond is connected by interface channel with main channel；Described waste liquid pool is connected by interface channel with last reaction detection pond；
Described cover plate is to the sealing structure in addition to described sample holes and passage.
Described liquid storage tank is near chip center's fixing hole, and volume is total with interface channel volume more than all reaction detection ponds
With.
Described main channel is spirality, the outside of main channel, caudad uniform from spiral center and connect multiple reaction inspections
Pond is surveyed, the outside of described main channel is remote from chip center direction.
The number in described reaction detection pond is 5~20, is evenly distributed on the concentric circles of chip, each reaction detection
Pond is connected by an interface channel with main channel, and the volume in the reaction detection pond is 5~25 μ L, internal embedding reaction in advance
Detection reagent, the investment fashion includes following at least one, directly the immobilization detection reagent in the chip base, or
Person, the immobilization detection reagent on solid phase carrier, the solid phase carrier includes commercialization filter paper, perforated membrane, designed, designed gel
Film.
Last described reaction detection pond, side is connected by interface channel with spirality main channel tail end, separately
Side is connected by interface channel with waste liquid pool.
Described chip basal body is double-layer structure, including cover layer and channel layer.
Due to taking above technical scheme, it has advantages below to the utility model：1st, centrifugal routine urinalysis detection miniflow
Control chip is closed except sample holes and passage external structure, and sample is difficult to leak, and is difficult to pollute external equipment and environment；2nd, with centrifugation
The mode of sample introduction evenly distributes detected sample, and reaction detection pond is independent cell, can avoid in each reaction detection pond
Interfered between product；3rd, amount of samples is few, if liquid storage tank is filled it up with, and without using test tube, it is easy to operate；
4th, by setting waste liquid pool, buffered because of user's operation or the different caused sample injection volume differences of sample injector, so as to avoid
Cause indivedual reaction detection ponds discontented or excess liq from passage overflow the problems such as.
Brief description of the drawings
Fig. 1 is the top view that a kind of centrifugal routine urinalysis detects micro-fluidic chip.
In accompanying drawing：1. chip basal body, 2. center fixing hole, 3. sample holes, 4. liquid storage tank, 5. main channel, 6. interface channel,
7. reaction detection pond, 8. waste liquid pool, 9. passage.
Specific embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the utility model embodiment is carried out
Clearly it is fully described by.Obviously, described embodiment is only a part of embodiment of the utility model, rather than whole realities
Apply example.Based on the embodiment in the utility model, those of ordinary skill in the art institute under the premise of creative work is not made
The every other embodiment for obtaining, belongs to the scope of the utility model protection.
As shown in figure 1, described micro-fluidic chip includes chip basal body [1], center fixing hole [2], sample holes [3], storage
Liquid pool [4], main channel [5], interface channel [6], reaction detection pond [7], waste liquid pool [8], passage [9]；Described chip base
Body [1] has three layers, including cover layer, center-aisle layer, backplane level；Described center fixing hole [2] is positioned at chip basal body geometry
Center and through chip basal body [1]；Described sample holes [3] with passage [9] in cover layer, described sample holes [3]
It is connected with liquid storage tank [4], described passage [9] is connected by interface channel [6] with waste liquid pool [8]；Described liquid storage tank
[4] connected with main channel [5]；Described reaction detection pond [7] is connected by interface channel [6] with main channel [5]；Described is useless
Liquid pool [8] is connected by interface channel [6] with last reaction detection pond [7]；Described cover plate is to except described sample holes
[3] and the outer sealing structure of passage [9].
In above-described embodiment, liquid storage tank [4] is more than all reaction detection ponds [7] near chip center's fixing hole [2], volume
With interface channel [6] volume summation.
In above-described embodiment, main channel [5] are spirality, the outside of main channel [5], i.e., away from chip center direction, from
Spiral center is caudad uniform and connects multiple reaction detection ponds [7].
In above-described embodiment, the number of reaction detection pond [7] is 5~20, is evenly distributed on the concentric circles of chip, often
Individual reaction detection pond [7] is connected by an interface channel [6] with main channel [5], and the volume in the reaction detection pond is 5~25
μ L, internal embedding reaction detection reagent in advance, the investment fashion includes following at least one, directly in the chip base
Immobilization detection reagent, or, the immobilization detection reagent on solid phase carrier, the solid phase carrier includes commercialization filter paper, many
Pore membrane, designed, designed gel mould.
In above-described embodiment, last reaction detection pond [7], side is by interface channel [6] and spirality main channel
[5] tail end is connected, and opposite side is connected by interface channel [6] with waste liquid pool [8].
In above-described embodiment, chip basal body [1] is double-layer structure, including cover layer and channel layer.
Below with routine urinalysis 11 (proportion, occult blood, leucocyte, nitrite, pH value, UBG, bilirubin, protein,
Glucose, ketoboidies, ascorbic acid) detection as a example by, illustrate that it uses process.12 reaction detection ponds are set, wherein 1 is blank
Compare, in addition 11 reaction reagents for embedding routine urinalysis 11 respectively.When using, with sample injector from sample holes [3] to micro-fluidic
Add urine to be checked to being just full of in the liquid storage tank [4] of chip, chip is put into relevant device, be centrifuged, by liquid storage tank [4]
In urine be centrifuged to reaction detection pond [7], carry out the reaction of each index of routine urinalysis and detected with result, draw different Testing index
Result.The reaction detection pond of varying number can be set in micro-fluidic chip, the detection of varying number index is met.
In above-described embodiment, centrifugal routine urinalysis detection micro-fluidic chip is a kind of general chip, normal except that can make urine
Rule detection is outer, may also extend into the fields such as clinical biochemical, food security, environment measuring.
In above-described embodiment, the utility model is merely to illustrate, wherein the structure of each part, set location and its connection side
Formula etc. all can be what is be varied from, every equivalents carried out on the basis of technical solutions of the utility model and improvement,
Should not exclude outside protection domain of the present utility model.
Claims (6)
1. a kind of centrifugal routine urinalysis detects micro-fluidic chip, it is characterised in that：Described micro-fluidic chip include chip basal body,
Center fixing hole, sample holes, liquid storage tank, main channel, interface channel, reaction detection pond, waste liquid pool, passage；Described chip
Matrix has three layers, including cover layer, center-aisle layer, backplane level；Described center fixed hole position is in chip basal body geometric center
And through chip basal body；Described sample holes are located in cover layer with passage, and described sample holes are connected with liquid storage tank, institute
The passage stated is connected by interface channel with waste liquid pool；Described liquid storage tank is connected with main channel；Described reaction detection pond
Connected with main channel by interface channel；Described waste liquid pool is connected by interface channel with last reaction detection pond；Institute
The cover plate stated is to the sealing structure in addition to described sample holes and passage.
2. a kind of centrifugal routine urinalysis according to claim 1 detects micro-fluidic chip, it is characterised in that：Described liquid storage
Near chip center's fixing hole, volume is more than all reaction detection ponds and interface channel volume summation in pond.
3. a kind of centrifugal routine urinalysis according to claim 1 detects micro-fluidic chip, it is characterised in that：Described master is led to
Road is spirality, the outside of main channel, caudad uniform from spiral center and connect multiple reaction detection ponds, described main channel
Outside, is remote from chip center direction.
4. a kind of centrifugal routine urinalysis according to claim 1 detects micro-fluidic chip, it is characterised in that：Described reaction
The number of detection cell is 5~20, is evenly distributed on the concentric circles of chip, and each reaction detection pond passes through an interface channel
Connected with main channel, the volume in the reaction detection pond is 5~25 μ L, internal embedding reaction detection reagent, the embedding in advance
Mode includes following at least one, directly the immobilization detection reagent in the chip base, or, it is fixed on solid phase carrier
Change detection reagent, the solid phase carrier includes commercialization filter paper, perforated membrane, designed, designed gel mould.
5. a kind of centrifugal routine urinalysis according to claim 1 detects micro-fluidic chip, it is characterised in that：Described is last
One reaction detection pond, side is connected by interface channel with spirality main channel tail end, opposite side by interface channel with
Waste liquid pool is connected.
6. a kind of centrifugal routine urinalysis according to claim 1 detects micro-fluidic chip, it is characterised in that：Described chip
Matrix is double-layer structure, including cover layer and channel layer.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201620940803.XU CN206229378U (en) | 2016-08-24 | 2016-08-24 | A kind of centrifugal routine urinalysis detection micro-fluidic chip |
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| CN201620940803.XU CN206229378U (en) | 2016-08-24 | 2016-08-24 | A kind of centrifugal routine urinalysis detection micro-fluidic chip |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107389549A (en) * | 2017-08-17 | 2017-11-24 | 武汉璟泓万方堂医药科技股份有限公司 | Rotating disc type collaurum/fluorescent test paper chip |
| CN108051393A (en) * | 2018-01-18 | 2018-05-18 | 付豪 | A kind of intelligent closestool of full automatic urine detection device and the application device |
| CN109765391A (en) * | 2019-03-14 | 2019-05-17 | 杭州霆科生物科技有限公司 | A kind of centrifugal test strips chip of multiple determination |
| CN109894165A (en) * | 2019-03-14 | 2019-06-18 | 杭州霆科生物科技有限公司 | A kind of three joint inspection micrometer fluidic chip of clenbuterol hydrochloride |
| CN111077319A (en) * | 2019-12-18 | 2020-04-28 | 华南农业大学 | Micro-fluidic chip immunodetection kit and detection method thereof |
| CN111812085A (en) * | 2020-07-27 | 2020-10-23 | 重庆大学 | Fermented yellow water detection device |
| WO2022116758A1 (en) * | 2020-12-01 | 2022-06-09 | 南京岚煜生物科技有限公司 | Multi-functional microfluidic test chip |
-
2016
- 2016-08-24 CN CN201620940803.XU patent/CN206229378U/en active Active
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107389549A (en) * | 2017-08-17 | 2017-11-24 | 武汉璟泓万方堂医药科技股份有限公司 | Rotating disc type collaurum/fluorescent test paper chip |
| CN108051393A (en) * | 2018-01-18 | 2018-05-18 | 付豪 | A kind of intelligent closestool of full automatic urine detection device and the application device |
| CN108051393B (en) * | 2018-01-18 | 2023-10-20 | 付豪 | Full-automatic urine detection device and intelligent closestool using same |
| CN109765391A (en) * | 2019-03-14 | 2019-05-17 | 杭州霆科生物科技有限公司 | A kind of centrifugal test strips chip of multiple determination |
| CN109894165A (en) * | 2019-03-14 | 2019-06-18 | 杭州霆科生物科技有限公司 | A kind of three joint inspection micrometer fluidic chip of clenbuterol hydrochloride |
| CN109894165B (en) * | 2019-03-14 | 2021-05-18 | 杭州霆科生物科技有限公司 | Clenbuterol triple detection micro-fluidic chip |
| CN111077319A (en) * | 2019-12-18 | 2020-04-28 | 华南农业大学 | Micro-fluidic chip immunodetection kit and detection method thereof |
| CN111812085A (en) * | 2020-07-27 | 2020-10-23 | 重庆大学 | Fermented yellow water detection device |
| WO2022116758A1 (en) * | 2020-12-01 | 2022-06-09 | 南京岚煜生物科技有限公司 | Multi-functional microfluidic test chip |
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