CN103543279B - Portable quantitative detecting device for blood - Google Patents
Portable quantitative detecting device for blood Download PDFInfo
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- CN103543279B CN103543279B CN201310429422.6A CN201310429422A CN103543279B CN 103543279 B CN103543279 B CN 103543279B CN 201310429422 A CN201310429422 A CN 201310429422A CN 103543279 B CN103543279 B CN 103543279B
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- blood
- sample
- feeding pipe
- specimen collection
- needle tubing
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- 239000008280 blood Substances 0.000 title claims abstract description 76
- 210000004369 blood Anatomy 0.000 title claims abstract description 75
- 238000006243 chemical reaction Methods 0.000 claims abstract description 31
- 238000001514 detection method Methods 0.000 claims abstract description 10
- 230000002441 reversible effect Effects 0.000 claims abstract description 4
- 238000012360 testing method Methods 0.000 claims description 24
- 210000002381 plasma Anatomy 0.000 claims description 11
- 238000001914 filtration Methods 0.000 claims description 9
- 238000001228 spectrum Methods 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 5
- 239000003146 anticoagulant agent Substances 0.000 claims description 4
- 229940127219 anticoagulant drug Drugs 0.000 claims description 4
- 239000012780 transparent material Substances 0.000 claims description 4
- 239000011521 glass Substances 0.000 claims description 3
- 239000000523 sample Substances 0.000 description 70
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 18
- 239000003153 chemical reaction reagent Substances 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 4
- 235000012000 cholesterol Nutrition 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- 150000002632 lipids Chemical class 0.000 description 3
- HSINOMROUCMIEA-FGVHQWLLSA-N (2s,4r)-4-[(3r,5s,6r,7r,8s,9s,10s,13r,14s,17r)-6-ethyl-3,7-dihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1h-cyclopenta[a]phenanthren-17-yl]-2-methylpentanoic acid Chemical compound C([C@@]12C)C[C@@H](O)C[C@H]1[C@@H](CC)[C@@H](O)[C@@H]1[C@@H]2CC[C@]2(C)[C@@H]([C@H](C)C[C@H](C)C(O)=O)CC[C@H]21 HSINOMROUCMIEA-FGVHQWLLSA-N 0.000 description 2
- 239000000592 Artificial Cell Substances 0.000 description 2
- 230000003143 atherosclerotic effect Effects 0.000 description 2
- 239000003613 bile acid Substances 0.000 description 2
- 239000012472 biological sample Substances 0.000 description 2
- 238000009534 blood test Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000012113 quantitative test Methods 0.000 description 2
- 239000000376 reactant Substances 0.000 description 2
- 230000000630 rising effect Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000003270 steroid hormone Substances 0.000 description 2
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000004159 blood analysis Methods 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 208000014951 hematologic disease Diseases 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000003907 kidney function Effects 0.000 description 1
- 230000037356 lipid metabolism Effects 0.000 description 1
- 230000003908 liver function Effects 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 125000005457 triglyceride group Chemical group 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
Landscapes
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Hydrology & Water Resources (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a portable quantitative detecting device for blood. The device sequentially comprises a blood sample collecting part, a sample introduction part and a reaction tank from top to bottom, wherein the blood sample collecting part is composed of a blood sample collecting needle tube and a needle tube fixing stopper, the blood sample collecting needle tube passes through and is fixed on the needle tube fixing stopper, the lower part of the needle tube fixing stopper is provided with a cavity, and the side of the cavity is provided with a disconnecting/connecting hole; the sample introduction part is composed of a sample introduction tube, a horizontal disconnecting tube and a sample introduction tube fixing stopper, the sample introduction tube fixing stopper is sleeved with the needle tube fixing stopper, one end of the horizontal disconnecting tube is connected into a T shape together with the sample introduction tube and can wholly rotate along with the sample introduction tube fixing stopper, and the horizontal disconnecting tube is communicated with the disconnecting/connecting hole through rotation, so that the sample introduction tube and the horizontal disconnecting tube are communicated with the atmosphere; the wall of the reaction tank has certain elasticity, so that the deformation is reversible. The device disclosed by the invention is portable, simple in operation, low in sample consumption and capable of favorably meeting the requirement for quantitative detection of clinical blood samples.
Description
Technical field
The present invention relates to a kind of biological sample quantitative testing device, specifically a kind of portable blood quantitative testing device, belong to medical treatment and detect apparatus technical field.
Background technology
Blood test is one of modal chemical examination content of hospital, mainly through blood analysis, determines the situations such as human body anaemia, cholesterol, diabetes, various infection, renal function, calcium, liver function, hematologic disease and body exception.When biochemistry detection is carried out to blood sample, generally first need remove the cell in blood, then quantitative test be carried out to the various materials in blood plasma.Such as, lipid examination is mainly to a kind of method for quantitatively determining that lipid contained in blood plasma is carried out.Blood fat is extensively present in human body, is the basic metabolism material of human body cell.The Main Ingredients and Appearance of blood fat is triglyceride and cholesterol, and wherein, the metabolism of triglyceride participant energy i (in vivo), cholesterol is then mainly used in synthetic cell serous coat, steroid hormone and bile acid.Can the situation of antimer lipid metabolism to the quantitative testing result of blood fat.Because plasma cholesterol is relevant with atherosclerotic generation with the rising of triglyceride level, these two main projects having become lipid determination.Therefore, develop a kind of portable blood quantitative testing device to have very important significance.
Current existing blood sample quantitative testing device complicated operation, expensive, and volume is doted on greatly, consume sample many, be difficult to meet the demand of the particularly clinical field of each field instantly.
Summary of the invention
For existing blood test device in the weak point manufactured and exist in use procedure, the invention provides a kind of portable blood quantitative testing device, this device has easy to carry, simple to operate and that sample consumption is few advantage, can well meet the demand that clinical blood sample amounts detects.
The object of the present invention is achieved like this:
A kind of portable blood quantitative testing device, it comprises blood specimen collection part, sample introduction part and reaction tank from top to down according to this; Described blood specimen collection part is made up of blood specimen collection needle tubing, needle tubing fixed plug, and blood specimen collection needle tubing passes and is fixed in needle tubing fixed plug, and needle tubing fixed plug bottom is a cavity, and the sidepiece of described cavity is provided with blocking-up connector; Described sample introduction part is made up of sample feeding pipe, blocking-up transverse pipe, sample feeding pipe fixed plug, sample feeding pipe fixed plug is fixed plug sleeve by described needle tubing and is connected, the one end and the sample feeding pipe that block transverse pipe connect into T-shaped, and can with sample feeding pipe fixed plug integral-rotation, by rotating, blocking-up transverse pipe is connected with described blocking-up connector, thus sample feeding pipe is communicated with air with blocking-up transverse pipe; Described reaction pool wall is flexible, can reversible deformation.
Preferably, portable blood quantitative testing device as above, wherein said sample feeding pipe is transparent material, and end indicates scale.
Preferably, portable blood quantitative testing device as above, be provided with filtration members between wherein said blood specimen collection part and sample introduction part, this filtration members, by blood plasma, filters haemocyte.
Again further preferably, portable blood quantitative testing device as above, wherein said blood specimen collection needle tubing is glass or plastic material, and inwall applies anti-coagulants.
Again further preferably, portable blood quantitative testing device as above, wherein said blood specimen collection part is provided with over cap.
Again further preferably, portable blood quantitative testing device as above, wherein said blood specimen collection part stopped draw-in groove and was connected with over cap.
Portable blood quantitative testing device of the present invention, wherein said pick-up unit can also comprise the matching used spectrum detection instrument with reaction tank.
Compared with prior art, the portable blood quantitative testing device tool that the present invention relates to has the following advantages and marked improvement: blood sample pre-service, quantitative sample injection and reaction are integrated in one by the present invention, negative pressure is produced by extruding reaction pool wall, draw blood sample enters sample feeding pipe by filtration members, after a certain amount of sample enters, sample feeding pipe rotation being made it be connected with air can make sample enter reaction tank, blocks simultaneously and continues sample introduction, achieve quantitative sampling; After reagent in reaction tank and plasma sample complete reaction, apparatus of the present invention entirety can be put into the quantitative detection that matching used spectrum detection instrument carries out sample.As can be seen here, whole device volume of the present invention is little, it is convenient, simple to operate to carry, and can realize quantitative sampling, and sample is without waste.
Accompanying drawing explanation
Fig. 1 is apparatus of the present invention element composition diagram;
Fig. 2 is axial plane sectional structure chart in apparatus of the present invention;
Fig. 3 is the overall appearance schematic diagram of apparatus of the present invention;
In figure: 1-over cap, 2-blood specimen collection needle tubing, 3-needle tubing fixed plug, 4-filtration members, 5-sample feeding pipe fixed plug, 6-sample feeding pipe, 7-reaction tank, 8-reaction reagent, 9-sample feeding pipe scale mark, 10-blocks transverse pipe, and 11-sample feeding pipe blocks mouth.
Embodiment
Below in conjunction with accompanying drawing the present invention done and describe in detail further.
Portable blood quantitative testing device of the present invention, as shown in Figure 1 and Figure 2, it arranges blood specimen collection part, filtration members, sample introduction part and reaction tank from top to down according to this; Wherein:
Blood specimen collection part is made up of blood specimen collection needle tubing 2, needle tubing fixed plug 3, and blood specimen collection needle tubing 2 passes needle tubing fixed plug 3 and is fixed in needle tubing fixed plug 3, and needle tubing fixed plug 3 bottom is a cavity, and the sidepiece of this cavity is provided with and blocks connector 11; Blood specimen collection needle tubing 2 is glass or plastic material, and inwall applies anti-coagulants, in case blood sample solidifies.In addition, blood specimen collection part is connected with over cap 1 by draw-in groove.
Filtration members 4 has been sample pretreatment effects, and for the filtration of blood sample, haemocyte, not by this filter membrane, only has blood plasma to pass through, and optionally takes the filter membrane filtered in common biological sample solution, also optionally takes the filter membrane filtered in blood sample.
Sample introduction part is made up of sample feeding pipe 6, blocking-up transverse pipe 10, sample feeding pipe fixed plug 5, sample feeding pipe fixed plug 5 is overlapped company by needle tubing fixed plug 3, and (needle tubing fixed plug bottom is cavity, this cavity can entangle sample feeding pipe fixed plug, thus realize cover even), the one end and the sample feeding pipe 6 that block transverse pipe 10 connect into T-shaped, and with sample feeding pipe fixed plug 5 integral-rotation, by rotating, blocking-up transverse pipe 10 can be connected with blocking-up connector 11, thus sample feeding pipe 6 is communicated with air with blocking-up transverse pipe 10; Sample feeding pipe is transparent material, and end indicates scale.
Reaction tank 7, and can the soda acid of resistance to some strength for having the container of transparent material, and reaction pool wall is flexible, can reversible deformation, is preinstalled with the biochemical reagents 8 for reacting in reaction tank 7.
Further, matching used spectrum detection instrument can portable use, its sample storage unit and apparatus of the present invention in the same size, and can, according to the difference of detected object and biochemical reaction system, adopt different wave bands to detect.
Portable blood quantitative testing device of the present invention in use, negative pressure is produced by extruding reaction pool wall, draw blood sample enters sample feeding pipe by filter membrane, after a certain amount of sample enters, sample feeding pipe is rotated and is connected i.e. continuation sample introduction capable of blocking with air, after reaction reagent in blood sample reaction tube completes reaction, device entirety can be put into the quantitative detection that matching used spectrum detection instrument carries out sample.
Below by a concrete embodiment, the present invention is described further.
Embodiment 1: the content detecting patient's Blood Cholesterol.
Cholesterol in human body is mainly used in synthetic cell serous coat, steroid hormone and bile acid.Because the rising of plasma cholesterol is relevant with atherosclerotic generation, the content of Blood Cholesterol has become an important instruction of health.
Cholesterol level detects:
(1) reactant is added: before device assembling, can join in blood sample reaction tube with the reactant reagent of specific lentochol reaction.
(2) device assembling: device is assembled into shown in Fig. 3 by Fig. 1 order.
(3) sample: make its deformation with finger extruding blood sample reaction tube, use the negative pressure absorbing blood samples of patients produced when blood sample reaction tube recovers deformation, now, the anti-coagulants that blood and sample cell inwall apply reacts, and guarantee blood sample does not solidify.
(4) sample introduction: under suction function, blood sample is through membrane filtration cell, and blood plasma flows into sample feeding pipe; When estimating sample and flowing to the specific scale of sample feeding pipe end, by sample introduction part integral-rotation 180 degree, make blocking-up transverse pipe and sample introduction block connector and connect, now block transverse pipe and be directly communicated with air, blood plasma in longitudinal sample feeding pipe is injected reaction tank by air, and sample introduction stops.
(5) detect: flow into after the reaction reagent solution pre-installed in the quantitative test plasma sample of blood sample reaction tube and blood sample reaction tube fully reacts, whole device can be put into matching used spectrum detection instrument and quantitatively detect.
Those skilled in the art will readily understand; the foregoing is only preferred embodiment of the present invention; not in order to limit the present invention, all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.
Claims (7)
1. a portable blood quantitative testing device, it comprises blood specimen collection part, sample introduction part and reaction tank from top to down according to this; Described blood specimen collection part is made up of blood specimen collection needle tubing, needle tubing fixed plug, and blood specimen collection needle tubing passes and is fixed in needle tubing fixed plug, it is characterized in that, described needle tubing fixed plug bottom is a cavity, and the sidepiece of described cavity is provided with blocking-up connector; Described sample introduction part is made up of sample feeding pipe, blocking-up transverse pipe, sample feeding pipe fixed plug, sample feeding pipe fixed plug is fixed plug sleeve by described needle tubing and is connected, the one end and the sample feeding pipe that block transverse pipe connect into T-shaped, and can with sample feeding pipe fixed plug integral-rotation, by rotating, blocking-up transverse pipe is connected with described blocking-up connector, thus sample feeding pipe is communicated with air with blocking-up transverse pipe; Described reaction pool wall is flexible, can reversible deformation.
2. portable blood quantitative testing device according to claim 1, is characterized in that: described sample feeding pipe is transparent material, and end indicates scale.
3. portable blood quantitative testing device according to claim 1, is characterized in that: be provided with filtration members between described blood specimen collection part and sample introduction part, this filtration members, by blood plasma, filters haemocyte.
4. portable blood quantitative testing device according to claim 1, is characterized in that: described blood specimen collection needle tubing is glass or plastic material, and inwall applies anti-coagulants.
5. the portable blood quantitative testing device according to any one of claim 1-3, is characterized in that: described blood specimen collection part is provided with over cap.
6. portable blood quantitative testing device according to claim 5, is characterized in that: described blood specimen collection part stopped draw-in groove and is connected with over cap.
7. the portable blood quantitative testing device according to any one of claim 1-3, is characterized in that: described pick-up unit also comprises the matching used spectrum detection instrument with reaction tank.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310429422.6A CN103543279B (en) | 2013-09-22 | 2013-09-22 | Portable quantitative detecting device for blood |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310429422.6A CN103543279B (en) | 2013-09-22 | 2013-09-22 | Portable quantitative detecting device for blood |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103543279A CN103543279A (en) | 2014-01-29 |
| CN103543279B true CN103543279B (en) | 2015-05-13 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310429422.6A Active CN103543279B (en) | 2013-09-22 | 2013-09-22 | Portable quantitative detecting device for blood |
Country Status (1)
| Country | Link |
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| CN (1) | CN103543279B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015138818A1 (en) * | 2014-03-12 | 2015-09-17 | Theranos, Inc. | Systems, devices, and methods for bodily fluid sample collection |
| CN107764881A (en) * | 2017-11-27 | 2018-03-06 | 民康医疗科技（天津）有限公司 | A kind of portable blood test kit device based on electrochemical method |
| CN108872560A (en) * | 2018-08-14 | 2018-11-23 | 湖北伽诺美生物科技有限公司 | A kind of portable urine quantitative testing device |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU615292B2 (en) * | 1988-05-11 | 1991-09-26 | Du Pont Canada Inc. | Apparatus for separating phases of blood |
| CN2406614Y (en) * | 2000-01-05 | 2000-11-22 | 高炳源 | Disposable vacuum blood sampling syringe |
| CN1122500C (en) * | 2000-01-25 | 2003-10-01 | 高炳源 | Syringe type expendable negative-pressure (vacuum) hemostix |
| US7309468B2 (en) * | 2002-05-13 | 2007-12-18 | Becton, Dickinson And Company | Protease inhibitor sample collection system |
| US7378054B2 (en) * | 2004-04-16 | 2008-05-27 | Savvipharm Inc | Specimen collecting, processing and analytical assembly |
| GB201004072D0 (en) * | 2010-03-11 | 2010-04-28 | Turzi Antoine | Process, tube and device for the preparation of wound healant composition |
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2013
- 2013-09-22 CN CN201310429422.6A patent/CN103543279B/en active Active
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| Publication number | Publication date |
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| CN103543279A (en) | 2014-01-29 |
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Effective date of registration: 20170217 Address after: 430074 Wuhan high tech Avenue, No. 666 biological city innovation park, building C6, North building, building, No. 402, No. Patentee after: Wuhan Ganuo beauty Technology Co. Ltd. Address before: 430071 Hubei Province, Wuhan Province, No. 17 South Road, Hubei Province Department of water resources, building 2, unit 701, 7 Patentee before: Feng Xiaojun |
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