CN103543103B - A kind of Portable biological sample quantitative detection device - Google Patents
A kind of Portable biological sample quantitative detection device Download PDFInfo
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- CN103543103B CN103543103B CN201310429424.5A CN201310429424A CN103543103B CN 103543103 B CN103543103 B CN 103543103B CN 201310429424 A CN201310429424 A CN 201310429424A CN 103543103 B CN103543103 B CN 103543103B
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Abstract
The invention belongs to a kind of biological sample pick-up unit, specifically a kind of Portable biological sample quantitative detection device.The present invention includes sample storage part, filter membrane, sample introduction part, diaphragm seal, reaction tank.Sample to be detected adds in sample cell, after filtration after membrane filtration, by sample feeding pipe Quality control influx, last sample feeding pipe penetrates diaphragm seal makes sample flow into transparent reaction tank, react with solution existing in reaction tank, subsequently device being put into spectrum detection instrument quantitatively can detect sample.Apparatus of the present invention are portable, simple to operate, and sample consumption is few, can well meet the requirement that clinical sample detects or daily biological sample quantitatively detects.
Description
Technical field
The invention belongs to a kind of biological sample pick-up unit, specifically a kind of Portable biological sample quantitative detection device.
Background technology
Biochemical Lab or hospital often need carry out the quantitative detection of various biology or biological sample.Quantitative detection is most important for life science, such as enzyme activity determination, or the mensuration etc. of target protein or DNA content.In hospital various disease diagnostic procedure in, often need the various materials contained in blood quantitatively be detected, target sample detect before, first need remove the haemocyte in blood, then blood plasma is analyzed.Such as, tumor markers is the chemicals that reflection tumour exists, they or be not present in normal adult tissue and be detected in embryonic tissue, or the content in tumor tissues substantially exceeds the content in normal structure, their existence or quantitative change can point out the character of tumour, so as to understanding tissue generations of tumour, Cell Differentiation, cell function, to help the diagnosis of tumour, classification, Index for diagnosis and treatment guidance.And most of tumor markers is present in blood plasma, before quantitative measurement is carried out to it, pre-service need be carried out to blood sample, again some specific glycoprotein or glycolipid in blood plasma be detected after namely removing haemocyte.Therefore, develop a kind of portable device for fast detecting that can be used for biological sample to have very important significance.
Current existing biological sample quantitative testing device complicated operation, expensive, and volume is doted on greatly, consume sample many, be difficult to meet the demand of the particularly clinical field of each field instantly.
Summary of the invention
For the deficiency of background technology, the present invention includes sample storage part, filter membrane, sample introduction part, diaphragm seal, reaction tank.Sample to be detected adds in sample cell, after filtration after membrane filtration, by sample feeding pipe Quality control influx, last sample feeding pipe penetrates diaphragm seal makes sample flow into transparent reaction tank, react with solution existing in reaction tank, subsequently device being put into spectrum detection instrument quantitatively can detect sample.Apparatus of the present invention are portable, simple to operate, and sample consumption is few, can well meet the requirement that clinical sample detects or daily biological sample quantitatively detects.
Technical scheme of the present invention is: a kind of Portable biological sample quantitative detection device, comprise sample storage part respectively from top to bottom, filter membrane, sample introduction part, diaphragm seal, reaction tank, sample storage part is made up of enclosing cover and sample cell, it is characterized in that: when cover top portion slightly great strength pressing, the sample feeding pipe that diaphragm seal can be arranged on sample introduction part pierces through.
Pick-up unit as above, it is characterized in that: described sample introduction part by block connector, transverse pipe, sample feeding pipe, sample introduction tube wall shell fragment, spring and be arranged on the upper latch notch on outer wall, lower bayonet socket forms, sample feeding pipe lower end is acute taper, sample feeding pipe upper end and transverse pipe connect into T-shaped, and can integral-rotation, make blocking-up transverse pipe by rotating and block connector and be connected; Spring and outer wall are used for the fixing of sample feeding pipe, and sample introduction tube wall shell fragment is arranged between upper latch notch and lower bayonet socket.
Pick-up unit as above, is characterized in that: described sample feeding pipe is transparent material, which is provided with scale mark.
Pick-up unit as above, is characterized in that: described diaphragm seal adopts rubber membrane.
Pick-up unit as above, is characterized in that: it also comprises the draw-in groove for fixing enclosing cover.
Pick-up unit as above, is characterized in that: described sample cell inwall scribbles and covers anti-coagulants.
Technique effect of the present invention is embodied in: sample pretreatment, quantitative sample injection and reaction become one by the present invention; Utilize the negative pressure in sample cell, when sample feeding pipe punctures caulking gum film, make sample enter sample feeding pipe, after a certain amount of sample enters, sample feeding pipe is rotated and is connected can enters reaction tank by test agent with air, block simultaneously and continue sample introduction; After reactant in reaction tank completes reaction, device entirety can be put into the quantitative detection that matching used spectrum detection instrument carries out sample.
Accompanying drawing explanation
Fig. 1 is apparatus of the present invention element composition diagram;
Device one-piece construction figure before Fig. 2 (A) sample introduction;
Axial plane sectional view in device before Fig. 2 (B) sample introduction;
Fig. 3 (A) sample introduction timer one-piece construction figure;
Axial plane sectional view in Fig. 3 (B) sample introduction timer.
Embodiment
Description of reference numerals: 1-sample storage part, 2-filter membrane, 3-sample introduction part, 4-diaphragm seal, 5-reaction tank, 6-reactant, 7-enclosing cover, 8-sample cell, 9-enclosing cover draw-in groove, 10-blocking connector, 11-transverse pipe, (its end has scale to 12-longitudinal sample feeding pipe, before sample introduction, the taper mouth of pipe in sample feeding pipe lower end is positioned at above O-ring seal), 13-sample introduction tube wall shell fragment, 14-lower bayonet socket, 15-scale mark, 16-spring, 17-upper latch notch
Below in conjunction with accompanying drawing the present invention done and describe in detail further.
Portable biometric sample quantitative detection device of the present invention, as shown in Figure 1, this device arranges sample storage part 1 from top to bottom respectively, filter membrane 2, sample introduction part 3, diaphragm seal 4, reaction tank 5, wherein reactant 6 is the reactants joining reaction tank before sample introduction, and it and testing sample specific reaction can occur, and product can be measured by ultraviolet or fluorescence spectrophotometer.
Filter membrane 2 of the present invention is optional takes the filter membrane filtered in common biological sample solution, also optional filter membrane of taking in blood sample filtration.Diaphragm seal 4 in the present invention adopts rubber membrane, seals before its sample introduction, can be punctured during sample introduction by the sharp-pointed lower end of sample feeding pipe above it 12.
Accompanying drawing 2(A) be depicted as device appearance schematic diagram before sample introduction; Fig. 2 (B) is device diagrammatic cross-section before sample introduction, and wherein sample storage part 1 is made up of enclosing cover 7 and sample cell 8, and sample cell 8 inwall can apply anti-coagulants, solidify for preventing blood sample, for the ease of fixing enclosing cover 7, also can set up enclosing cover draw-in groove 9, for fixing enclosing cover.Enclosing cover 7 can directly be annotated after opening sample in sample cell 8.
Sample introduction part 3 of the present invention by block connector 10, transverse pipe 11, sample feeding pipe 12, sample introduction tube wall shell fragment 13, spring 16 and be arranged on upper latch notch 17 on outer wall, lower bayonet socket 14 forms.Wherein, sample feeding pipe 12 lower end is designed to an acute taper, and sample feeding pipe 12 upper end and transverse pipe 11 connect into T-shaped, and can integral-rotation, makes blocking-up transverse pipe 11 by rotating and blocks connector 10 and be connected, thus makes sample feeding pipe 12 and transverse pipe 11 and atmosphere; Sample feeding pipe 12 is transparent material, which is provided with scale mark 15; Spring 16 and outer wall are used for the fixing of sample feeding pipe 12.Sample introduction tube wall shell fragment 13 of the present invention is arranged between upper latch notch 17 and lower bayonet socket 14, and when the slightly great strength pressing of enclosing cover 7 top, sample introduction tube wall shell fragment 13 will move to bayonet socket 14, simultaneously longitudinal sample feeding pipe 12 puncture through seal film 4 under outer wall by outer wall upper latch notch 17.
Below by a concrete embodiment, the present invention is described further.
Example 1: the content detecting specific glycolipid in cancer patient's blood.
High compared with ordinary people times of some specific glycolipid content in cancer patient's blood plasma, after ocal resection, these glycolipid content can decline, and as risen, then can represent recurrence again.
Glycolipid content detects:
(1) reactant is added: before device assembling, the reactant solution that can react with specific glycolipid joins in reaction tank 5, then by outside air extractor, reaction tank 5 is pumped into-0.5 atmospheric pressure, and seals with diaphragm seal 4;
(2) device assembling: device is assembled into accompanying drawing 2(A by accompanying drawing 1 order) shown in;
(3) application of sample: the blood directly adding a patient in sample cell 8, lid mounted device enclosing cover 7, now, the anti-coagulants that blood and sample cell 8 inwall apply reacts, and ensures that blood sample does not solidify;
(4) sample introduction: at device enclosing cover 7 top with slightly great strength pressing, sample introduction tube wall shell fragment 13 will move to bayonet socket 14 under outer wall by outer wall upper latch notch 17, longitudinal sample feeding pipe 12 puncture through seal film 4 simultaneously, extend into the negative pressure space of reaction tank, due to pressure differential, blood sample makes that haemocyte filters and blood plasma slowly flows down from sample cell 8 through longitudinal sample feeding pipe 12 through the effect of filter membrane 2; When range estimation flow to sample feeding pipe 12 specific scale 15 to sample, by sample introduction part integral-rotation 180 degree, make sample introduction blocking-up transverse pipe 11 block connector 10 with sample introduction and connect.Because longitudinal inlet pipe upper end is filter membrane 2, its resistance is comparatively large, and sample introduction blocking-up transverse pipe 11 is direct and air is connected, and its resistance is less, so the sample in sample cell 8 no longer flows down, the blood plasma simultaneously in sample feeding pipe 12 is injected reaction tank 5 by air pressure, and sample introduction stops.
(5) detect: flow into after existing reactant 6 solution fully reacts in the quantitative test plasma sample of sample inlet pool and reaction tank, whole device can be put into matching used spectrum detection instrument and quantitatively detect.
Those skilled in the art will readily understand; the foregoing is only preferred embodiment of the present invention; not in order to limit the present invention, all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.
Claims (5)
1. a Portable biological sample quantitative detection device, comprise sample storage part respectively from top to bottom, filter membrane, sample introduction part, diaphragm seal, reaction tank, sample storage part is made up of enclosing cover and sample cell, when cover top portion slightly great strength pressing, the sample feeding pipe that diaphragm seal can be arranged on sample introduction part pierces through, it is characterized in that: described sample introduction part is by blocking connector, transverse pipe, sample feeding pipe, sample introduction tube wall shell fragment, spring and the upper latch notch be arranged on outer wall, lower bayonet socket composition, sample feeding pipe lower end is acute taper, sample feeding pipe upper end and transverse pipe connect into T-shaped, and can integral-rotation, by rotating, blocking-up transverse pipe is connected with blocking-up connector, spring and outer wall are used for the fixing of sample feeding pipe, and sample introduction tube wall shell fragment is arranged between upper latch notch and lower bayonet socket.
2. pick-up unit as claimed in claim 1, is characterized in that: described sample feeding pipe is transparent material, which is provided with scale mark.
3. pick-up unit as claimed in claim 1, is characterized in that: described diaphragm seal adopts rubber membrane.
4. pick-up unit as claimed in claim 1, is characterized in that: it also comprises the draw-in groove for fixing enclosing cover.
5. pick-up unit as claimed in claim 1, is characterized in that: described sample cell inwall scribbles and covers anti-coagulants.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310429424.5A CN103543103B (en) | 2013-09-22 | 2013-09-22 | A kind of Portable biological sample quantitative detection device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310429424.5A CN103543103B (en) | 2013-09-22 | 2013-09-22 | A kind of Portable biological sample quantitative detection device |
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| CN103543103A CN103543103A (en) | 2014-01-29 |
| CN103543103B true CN103543103B (en) | 2016-01-20 |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3105574A4 (en) | 2014-02-11 | 2017-08-30 | The Board of Trustees of The Leland Stanford Junior University | Rapid small volume detection of blood ammonia |
| CN104209154A (en) * | 2014-08-27 | 2014-12-17 | 广东中测食品化妆品安全评价中心有限公司 | Detection test pipe and use method thereof |
| CN106706625A (en) * | 2017-01-19 | 2017-05-24 | 丁葆兰 | Disposable intestinal tumor screening detection cannula |
| CN110470808A (en) * | 2018-05-11 | 2019-11-19 | 中国石油化工股份有限公司 | Water quality many reference amounts detection device and detection method based on manual injector |
| CN108872560A (en) * | 2018-08-14 | 2018-11-23 | 湖北伽诺美生物科技有限公司 | A kind of portable urine quantitative testing device |
| CN112924394B (en) * | 2021-01-24 | 2022-10-25 | 张苗苗 | Adjustable food safety inspection device |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0341586A2 (en) * | 1988-05-11 | 1989-11-15 | Dupont Canada Inc. | Apparatus for separating phases of blood |
| CN1804602A (en) * | 2005-12-15 | 2006-07-19 | 卢麟麟 | Apparatus and method for quick detection of surface cleanness degree and microbe contamination |
| CN102252871A (en) * | 2011-07-05 | 2011-11-23 | 浙江友利医学科技有限公司 | One-time conveying sampler |
-
2013
- 2013-09-22 CN CN201310429424.5A patent/CN103543103B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0341586A2 (en) * | 1988-05-11 | 1989-11-15 | Dupont Canada Inc. | Apparatus for separating phases of blood |
| CN1804602A (en) * | 2005-12-15 | 2006-07-19 | 卢麟麟 | Apparatus and method for quick detection of surface cleanness degree and microbe contamination |
| CN102252871A (en) * | 2011-07-05 | 2011-11-23 | 浙江友利医学科技有限公司 | One-time conveying sampler |
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