CN103543103A

CN103543103A - Portable biological sample quantitative detection device

Info

Publication number: CN103543103A
Application number: CN201310429424.5A
Authority: CN
Inventors: 冯晓均
Original assignee: Individual
Current assignee: Individual
Priority date: 2013-09-22
Filing date: 2013-09-22
Publication date: 2014-01-29
Anticipated expiration: 2033-09-22
Also published as: CN103543103B

Abstract

The invention belongs to a biological sample detection device and in particular relates to a portable biological sample quantitative detection device. The portable biological sample quantitative detection device comprises a sample storage piece, a filter film, a sample injection piece, a sealing film and a reaction pool, wherein a sample to be detected is added into a sample pool; after the sample to be detected is filtered through the filter film, the sample injection pipe is used for controlling the sample injection amount; finally the sample injection pipe penetrates through the sealing film to allow the sample to flow into the transparent reaction pool, and the sample generates reaction with an existing solution in the reaction pool; the device is put into a spectroscopic detector for quantitatively detecting the sample. The portable biological sample quantitative detection device is portable and is easy to operate; the consumption of the sample is low; a requirement on clinical sample detection or daily biochemical sample quantitative detection can be well met.

Description

A kind of portable biochemical sample quantitative testing device

Technical field

The invention belongs to a kind of biological sample pick-up unit, specifically a kind of portable biochemical sample quantitative testing device.

Background technology

Biochemical Lab or hospital often need carry out the quantitative detection of various biologies or biological sample.Quantitatively detection is most important for life science, such as enzyme activity determination, or the mensuration of target protein or DNA content etc.In hospital, in the diagnostic procedure of various diseases, the various materials that often need contain blood quantitatively detect, and before target sample detects, need first remove the haemocyte in blood, then blood plasma is analyzed.For example, tumor markers is the chemical classes material that reflection tumour exists, they or be not present in normal adult tissue and be detected in embryonic tissue, or the content in tumor tissues substantially exceeds the content in normal structure, their existence or quantitative change can be pointed out the character of tumour, so as to understanding tissue generation, Cell Differentiation, the cell function of tumour, to help diagnosis, classification, prognosis judgement and the treatment of tumour to instruct.And most of tumor markers is present in blood plasma, before it is carried out to quantitative measurement, need carry out pre-service to blood sample, again some specific glycoprotein or glycolipid in blood plasma are detected after removing haemocyte.Therefore, developing a kind of portable device for fast detecting that can be used for biological sample has very important significance.

Current existing biological sample quantitative testing device complicated operation, expensive, and volume dotes on greatly, consumes sample many, be difficult to meet instantly the particularly demand of clinical field of each field.

Summary of the invention

For the deficiency of background technology, the present invention includes sample storage member, filter membrane, sample introduction part, diaphragm seal, reaction tank.Sample to be detected adds in sample cell, after filtration after membrane filtration, by sample feeding pipe Quality control influx, last sample feeding pipe penetrates diaphragm seal makes sample flow into transparent reaction tank, react with existing solution in reaction tank, subsequently device is put into spectrum detection instrument and can quantitatively be detected sample.Apparatus of the present invention are portable, simple to operate, and sample consumption is few, can well meet the requirement that clinical sample detects or daily biological sample quantitatively detects.

Technical scheme of the present invention is: a kind of portable biochemical sample quantitative testing device, comprise respectively from top to bottom sample storage member, filter membrane, sample introduction part, diaphragm seal, reaction tank, sample storage member is comprised of enclosing cover and sample cell, it is characterized in that: when cover top portion, by great strength slightly, press, the sample feeding pipe that diaphragm seal can be arranged on sample introduction part pierces through.

Pick-up unit as above, it is characterized in that: described sample introduction part by block connector, transverse pipe, sample feeding pipe, sample introduction tube wall shell fragment, spring and be arranged on the upper latch notch on outer wall, lower bayonet socket forms, sample feeding pipe lower end is sharp-pointed taper, sample feeding pipe upper end and transverse pipe connect into T-shaped, and can integral-rotation, by rotation, make to block transverse pipe and being connected with blocking-up connector; Spring and outer wall are fixing for sample feeding pipe, and sample introduction tube wall shell fragment is arranged between upper latch notch and lower bayonet socket.

Pick-up unit as above, is characterized in that: described sample feeding pipe is transparent material, which is provided with scale mark.

Pick-up unit as above, is characterized in that: described diaphragm seal adopts rubber membrane.

Pick-up unit as above, is characterized in that: it also comprises for the fixing draw-in groove of enclosing cover.

Pick-up unit as above, is characterized in that: on described sample cell inwall, scribble and cover anti-coagulants.

Technique effect of the present invention is embodied in: the present invention becomes one sample pretreatment, quantitative sample injection and reaction; Utilize the negative pressure in sample cell, when sample feeding pipe punctures caulking gum film, make sample enter sample feeding pipe, after a certain amount of sample enters, sample feeding pipe rotation is connected with atmosphere and can enters reaction tank by test agent, blocking-up simultaneously continues sample introduction; Reactant in reaction tank completes after reaction, device integral body can be put into the quantitative detection that matching used spectrum detection instrument carries out sample.

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Accompanying drawing explanation

Fig. 1 is apparatus of the present invention element composition diagram;

Device one-piece construction figure before Fig. 2 (A) sample introduction;

Axial plane sectional view in device before Fig. 2 (B) sample introduction;

Fig. 3 (A) sample introduction timer one-piece construction figure;

Axial plane sectional view in Fig. 3 (B) sample introduction timer.

Embodiment

Description of reference numerals: 1-sample storage member, 2-filter membrane, 3-sample introduction part, 4-diaphragm seal, 5-reaction tank, 6-reactant, 7-enclosing cover, 8-sample cell, 9-enclosing cover draw-in groove, 10-blocking-up connector, 11-transverse pipe, 12-(its end has scale to longitudinal sample feeding pipe, before sample introduction, the sample feeding pipe lower end taper mouth of pipe is positioned at O-ring seal top), 13-sample introduction tube wall shell fragment, 14-lower bayonet socket, 15-scale mark, 16-spring, 17-upper latch notch

Below in conjunction with accompanying drawing, the present invention is done further and described in detail.

Portable biometric sample quantitative testing device of the present invention, as shown in Figure 1, this device arranges respectively sample storage member 1 from top to bottom, filter membrane 2, sample introduction part 3, diaphragm seal 4, reaction tank 5, wherein reactant 6 is before sample introduction, to join the reactant of reaction tank, and specific reaction can occur for it and testing sample, and product can be measured by ultraviolet or fluorescence spectrophotometer.

Filter membrane 2 of the present invention is optional takes in the filter membrane of ordinary student matter sample solution filter, also the optional filter membrane filtering in blood sample of taking.Diaphragm seal 4 in the present invention adopts rubber membrane, before its sample introduction, seals, and during sample introduction, can be punctured by the sharp-pointed lower end of its top sample feeding pipe 12.

Accompanying drawing 2(A) be depicted as the front device appearance schematic diagram of sample introduction; Fig. 2 (B) is device diagrammatic cross-section before sample introduction, and wherein sample storage member 1 is comprised of enclosing cover 7 and sample cell 8, on sample cell 8 inwalls, can apply anti-coagulants, be used for preventing that blood sample from solidifying, for the ease of fixing enclosing cover 7, also can set up enclosing cover draw-in groove 9, for fixing enclosing cover.Enclosing cover 7 can be directly after opening toward the sample of annotating in sample cell 8.

Sample introduction part 3 of the present invention is comprised of upper latch notch 17, the lower bayonet socket 14 of blocking connector 10, transverse pipe 11, sample feeding pipe 12, sample introduction tube wall shell fragment 13, spring 16 and be arranged on outer wall.Wherein, sample feeding pipe 12 lower ends are designed to a sharp-pointed taper, and sample feeding pipe 12 upper ends and transverse pipe 11 connect into T-shaped, and can integral-rotation, make to block transverse pipe 11 and being connected with blocking-up connector 10, thereby make sample feeding pipe 12 and transverse pipe 11Yu atmosphere UNICOM by rotation; Sample feeding pipe 12 is transparent material, which is provided with scale mark 15; Spring 16 and outer wall are fixing for sample feeding pipe 12.Sample introduction tube wall shell fragment 13 of the present invention is arranged between upper latch notch 17 and lower bayonet socket 14, when enclosing cover 7 tops, by great strength slightly, presses, and sample introduction tube wall shell fragment 13 will move to bayonet socket 14 under outer wall by outer wall upper latch notch 17, simultaneously longitudinal sample feeding pipe 12 puncture through seal films 4.

Below by a concrete embodiment, the present invention is described further.

Example 1: the content that detects specific glycolipid in cancer patient's blood.

In cancer patient's blood plasma, some specific glycolipid content is compared with high times of ordinary people, and after ocal resection, these glycolipid content can decline, and as risen, can represent recurrence again.

Glycolipid content detection:

(1) add reactant: before device assembling, can join with the reactant solution that specific glycolipid reacts in reaction tank 5, and then by outside air extractor, reaction tank 5 will be pumped into-0.5 atmospheric pressure, and seal with diaphragm seal 4;

(2) device assembling: device is assembled into accompanying drawing 2(A by accompanying drawing 1 order);

(3) application of sample: in sample cell 8, directly add a patient's blood, lid mounted device enclosing cover 7, now, blood reacts with the anti-coagulants applying on sample cell 8 inwalls, guarantees that blood sample does not solidify;

(4) sample introduction: press by great strength slightly at device enclosing cover 7 tops, sample introduction tube wall shell fragment 13 will move to bayonet socket 14 under outer wall by outer wall upper latch notch 17, simultaneously longitudinal sample feeding pipe 12 puncture through seal films 4, extend into the negative pressure space of reaction tank, due to pressure differential, blood sample filters haemocyte and blood plasma slowly flows down through longitudinal sample feeding pipe 12 from sample cell 8 through the effect of filter membrane 2; When range estimation flow to sample feeding pipe 12 specific scale 15 to sample, by sample introduction part integral-rotation 180 degree, sample introduction blocking-up transverse pipe 11 and sample introduction blocking-up connector 10 are connected.Because longitudinal inlet pipe upper end is filter membrane 2, its resistance is larger, and sample introduction blocking-up transverse pipe 11 is direct and atmosphere is connected, and its resistance is less, so the sample in sample cell 8 no longer flows down, the blood plasma in sample feeding pipe 12 is by air pressure injection reaction tank 5 simultaneously, and sample introduction stops.

(5) detect: the plasma sample quantitative to be measured that flows into sample inlet pool, with after existing reactant 6 solution fully react in reaction tank, can be put into matching used spectrum detection instrument by whole device and quantitatively detect.

Those skilled in the art will readily understand; the foregoing is only preferred embodiment of the present invention; not in order to limit the present invention, all any modifications of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in protection scope of the present invention.

Claims

1. a portable biochemical sample quantitative testing device, comprise respectively from top to bottom sample storage member, filter membrane, sample introduction part, diaphragm seal, reaction tank, sample storage member is comprised of enclosing cover and sample cell, it is characterized in that: when cover top portion, by great strength slightly, press, the sample feeding pipe that diaphragm seal can be arranged on sample introduction part pierces through.

2. pick-up unit as claimed in claim 1, it is characterized in that: described sample introduction part by block connector, transverse pipe, sample feeding pipe, sample introduction tube wall shell fragment, spring and be arranged on the upper latch notch on outer wall, lower bayonet socket forms, sample feeding pipe lower end is sharp-pointed taper, sample feeding pipe upper end and transverse pipe connect into T-shaped, and can integral-rotation, by rotation, make to block transverse pipe and being connected with blocking-up connector; Spring and outer wall are fixing for sample feeding pipe, and sample introduction tube wall shell fragment is arranged between upper latch notch and lower bayonet socket.

3. pick-up unit as claimed in claim 1 or 2, is characterized in that: described sample feeding pipe is transparent material, which is provided with scale mark.

4. pick-up unit as claimed in claim 1 or 2, is characterized in that: described diaphragm seal adopts rubber membrane.

5. pick-up unit as claimed in claim 1 or 2, is characterized in that: it also comprises for the fixing draw-in groove of enclosing cover.

6. pick-up unit as claimed in claim 1 or 2, is characterized in that: on described sample cell inwall, scribble and cover anti-coagulants.