CN202710509U - Biological reaction vessel - Google Patents
Biological reaction vessel Download PDFInfo
- Publication number
- CN202710509U CN202710509U CN 201220121931 CN201220121931U CN202710509U CN 202710509 U CN202710509 U CN 202710509U CN 201220121931 CN201220121931 CN 201220121931 CN 201220121931 U CN201220121931 U CN 201220121931U CN 202710509 U CN202710509 U CN 202710509U
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- China
- Prior art keywords
- biological reaction
- reaction container
- sensor
- plastic wall
- dissolved oxygen
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Abstract
Provided is a biological reaction vessel. The biological reaction vessel comprises a plastic wall. The plastic wall limits a biological reaction chamber in the plastic wall and is provided with a hole penetrating through the plastic wall. A membrane support is fixed on the inner surface of the plastic wall and is provided with a cylindrical part which penetrates through the hole. A sensor window membrane is connected to the membrane support next to the hole. The sensor window membrane has high oxygen permeability and forms waterproof sealing together with the membrane support.
Description
Technical field
The application belongs to the biological reaction container field.
Background technology
Oxygen is to be subject to the greatly gas of concern, is because its effect in the circulation of all living organisms simply.Measurement to oxygen concentration or dividing potential drop in numerous application is important.In some applications, directly measure gox concentration.In other application, measure the oxygen concentration that is dissolved in the liquid.Understand that importantly term " dissolved oxygen DO " refers to gox soluble in water, and should be with it and as at hydrone (H
2O) fixed oxygen (combined oxygen) of finding in is obscured.
A kind of promising application of measuring dissolved oxygen DO is in biological sample.These biological samples can be the vitro samples in the laboratory, or the vivo sample among the patient.The dissolved oxygen DO of measuring in the biological sample provides important diagnostic message for medical personnel, and/or the information of rendeing a service about particular treatment.
Frequently, biological sample is included in bio-reactor/mixer, and the measurement of dissolved oxygen DO provides the important information about the state that is included in biomass wherein.
The utility model content
Biological reaction container is provided.This biological reaction container comprises plastic wall, and described plastic wall limits biological respinse chamber wherein, and has the hole of passing.Membrane support is fixed to the inside surface of plastic wall.Membrane support has the cylindrical part that passes described hole.The sensor fenestrated membrane is connected to the membrane support in next-door neighbour hole.The sensor fenestrated membrane has high oxygen permeability, but forms waterproof sealing with membrane support.
Therefore, the application relates to the biological reaction container in following every middle definition.
1. biological reaction container, described biological reaction container comprises:
Plastic wall; And
Oxygen sensing film, described oxygen sensing film is arranged to be close to described plastic wall, and described oxygen sensing film has known oxygen permeability.
2. project 1 described biological reaction container it is characterized in that described plastic wall comprises the hole of passing described plastic wall, and described oxygen sensing film is arranged to be close to described hole.
3. project 2 described biological reaction containers, it is characterized in that described biological reaction container also comprises the membrane support with the cylindrical part that passes described hole and the flange portion that is fixed to the inside surface of described plastic wall, wherein said membrane support makes described oxygen sensing film be connected with the inside surface of described plastic wall.
4. project 3 described biological reaction containers is characterized in that described biological reaction container also comprises the sensor body that is connected to described cylindrical part.
5. project 4 described biological reaction containers is characterized in that described sensor body is connected removably with described cylindrical part.
6. project 4 described biological reaction containers is characterized in that described biological reaction container also comprises sensing electrode, and described sensing electrode is disposed in the indoor of the described sensing film of next-door neighbour.
7. project 6 described biological reaction containers is characterized in that described biological reaction container also comprises analyzer, and described analyzer and described electrode operationally link to each other with based on by the dissolved oxygen DO in the current measurement biological sample of described electrode.
8. biological reaction container, described biological reaction container comprises:
Plastic wall, described plastic wall define biological respinse chamber wherein, and have the hole of passing described plastic wall;
Be fixed to the membrane support of described plastic wall, described membrane support has the cylindrical part that passes described hole; And
With the sensor fenestrated membrane that the described membrane support in the described hole of next-door neighbour is connected, described sensor fenestrated membrane has high oxygen permeability, but forms waterproof sealing with described membrane support.
9. project 8 described biological reaction containers is characterized in that described biological reaction container also comprises the ammeter dissolved oxygen sensor, and described ammeter dissolved oxygen sensor is disposed in the described cylindrical part of described membrane support.
10. project 9 described biological reaction containers is characterized in that described ammeter dissolved oxygen sensor penetrates described cylindrical part.
11. project 10 described biological reaction containers is characterized in that described biological reaction container also comprises analyzer, described analyzer and described dissolved oxygen sensor link to each other to measure the dissolved oxygen DO of the biological sample in the described biological reaction container.
Description of drawings
Fig. 1 is the sketch of the ammeter sensor (amperometric sensor) of exemplary prior art, adopts the embodiment of the present utility model of described ammeter sensor to be particularly useful.
Fig. 2 is the sketch according to the ammeter dissolved oxygen sensor of the dissolved oxygen content that is used for the biological sample in the measurement single-use bio-reactor of embodiment of the present utility model.
Fig. 3 is the sketch according to the conventional dissolved oxygen sensor of the dissolved oxygen DO that is used for the measurement biological sample of embodiment of the present utility model.
Fig. 4 is the diagrammatic cross-sectional view according to the sensor window support that is connected with wall or plastic sheet single-use bio-reactor/mixer embodiment of the present utility model.
Fig. 5 is the sketch that is connected the sensing film that closes with single-use bio-reactor mixer/wall of a container according to embodiment of the present utility model.
Fig. 6 is the sketch according to the dissolved oxygen sensor that is connected with single-use bio-reactor/mixer of embodiment of the present utility model.
Embodiment
Fig. 1 is the sketch of typical ammeter sensor, adopts the embodiment of the present utility model of this ammeter sensor to be particularly useful.Sensor 1 comprises sensor body 10 and sensing film 16, and sensor body 10 and 16 cooperations of sensing film provide the chamber that wherein maintains supporting electrolyte 12.Sensing electrode 14 is disposed in the indoor of next-door neighbour's sensing film 16.Sensor 1 is considered to the ammeter dissolved oxygen sensor.From nineteen sixties, such sensor has been developed for many application.The principle of ammeter lambda sensor is presented among Fig. 1.Particularly, sensing film 16 has the oxygen permeability of regulation, and its control diffuses through the flowing of oxygen molecule 20 of film 16.In case oxygen molecule arrives the inboard of sensing film 16, then it is because sensed electrode 14 reduction and the generation current signals of electrode reaction (being presented among Fig. 1).The partial pressure of oxygen at the amplitude of current signal and sensing film 16 places is proportional.
Fig. 2 is the sketch according to the ammeter dissolved oxygen sensor of the dissolved oxygen content that is used for the biological sample in the measurement single-use bio-reactor of embodiment of the present utility model.Dissolved oxygen sensor 40 is installed in the single-use bio-reactor 50 and is arranged to the electric current indication of the dissolved oxygen content of sampling 52.Sensor 40 links to each other with the dissolved oxygen analyzer, 54 of operation sensor 40.Analyzer 54 uses sensor 40 to measure the dissolved oxygen DO of sample 52 and reading or other suitable indications of sampling 52 dissolved oxygen contents.
Deposit contradiction in design at single-use character and the routine operation of bio-reactor 50 and between being inserted into dissolved oxygen sensor in the bio-reactor.According to embodiment of the present utility model, dissolved oxygen sensor do not need with the single-use bio-reactor in medium direct physical contact is arranged.In this way, can insert or remove dissolved oxygen sensor and do not need sterilization process.
Fig. 3 is the sketch according to the conventional dissolved oxygen sensor of the dissolved oxygen DO that is used for measurement test(ing) medium or biological sample of embodiment of the present utility model.Dissolved oxygen sensor 1 can be any suitable dissolved oxygen sensor, comprises the above dissolved oxygen sensor of describing about Fig. 1.Sensor 1 is mounted or otherwise is fixed in the sensor window support 60.Sensor window support 60 is soldered, bonding or otherwise be mechanically fixed to the wall 62 of bio-reactor/mixer 50.Hole 68 is passed wall 62 and is formed, and has the approximately equalised diameter of external diameter with the part 64 of sensor window support 60.Sensor window support 60 is soldered, bonding or otherwise be mechanically fixed to wall at the interface 62 between interface 66 and/or hole 68 and external diameter 64.The sensor window support 60 of multiple single-use bio-reactor can remove and be reinstalled to from the sensor window support 60 of multiple single-use bio-reactor to conventional dissolved oxygen sensor 1.Sensor window support 60 also comprises sensor window 70, and sensor window 70 is arranged to contact with test(ing) medium or biological sample 72 and preferably provide high oxygen to permeate.When the sensing film of conventional lambda sensor contacts with induction window 70, can measure the oxygen content of the medium that passes window 70.In one embodiment, sensor fenestrated membrane 70 is made by the material with high oxygen infiltration.Preferably, the oxygen permeability of sensor fenestrated membrane 70 is defined as: to have diameter be on 0.275 inch the ammeter sensor of sensing electrode the time when being installed in, and will produce at least 20 microamperes (μ A) on the sea level at 25 ℃ of films 70 in air.This electric current defines the oxygen flow that passes with this understanding film.When needed, the embodiment that shows about Fig. 3 allows dissolved oxygen sensor 1 to separate with container 50.During steam sterilizing or the gamma-ray irradiation to container 50, can simply sensor 1 be removed from container 50.In addition, can at any time sensor 1 be removed for calibration the process of not interrupting container 50 inside from container 50.
Fig. 4 is the diagrammatic cross-sectional view according to the sensor window support 60 that is connected with wall 62 or plastic sheet single-use bio-reactor/mixer 50 embodiment of the present utility model.Aspect the embodiment that in Fig. 4, shows many with in Fig. 3, show those are similar, and similar assembly has same numbering.Particularly, the plastic sheet of container or wall 62 are set up porose, and the size in described hole is formed into the external diameter 64 that roughly surpasses cylindrical part 78.Cylindrical part 78 links to each other with flange member 74, and flange member 74 provides the face 76 that is sealed to single-use bio-reactor 50 inside.Can use any suitable method sealing flange member 74, described method comprises that heat bonding/tackifier is bonding, mechanical means such as ultra-sonic welded, or other appropriate method.In addition, sensing film 70 being sealed to flange 74 connects to form waterproof.Particularly, when sensing film 70 is fixed to flange member 74, and flange member 74 is when being fixed to the inside surface of wall 62 of single-use bio-reactor 50, and the test(ing) medium that is included in the biological sample in the single-use bio-reactor can not spill.
In Fig. 4, sensing film 70 is incorporated in the single-use bio-reactor by the assembly that becomes membrane support 60.The sensing film 70 of bio-reactor, membrane support 60 and the mechanical system that plastic sheet 62 is bonding by heat bonding/tackifier or any other is suitable all link together.In addition, can improve in order to strengthen sensing film 70.In this structure, sensing film 70 can be sterilized with single-use bio-reactor/container by gamma-ray irradiation or other suitable modes.During using the single-use bio-reactor, electrolyte and sensing electrode can be installed not need extra sterilization process as shown in FIG. 4 for oxygenation measurement.
In a lot of embodiments, the sensing film typically will be made by the teflon with relatively low oxygen permeability (PTFE) or similar polymkeric substance.Most of other polymkeric substance such as polypropylene or tygon have the oxygen permeability of high some orders of magnitude.According at least one embodiment of the present utility model, as graphic in Fig. 5, the wall of plastic sheet that can be by making simply sensing film and single-use bio-reactor is bonding and the sensing film is incorporated in the single-use bio-reactor.In Fig. 5, to compare with the plastic sheet of single-use bio-reactor, the sensing film has relatively low oxygen permeability.To be the sensing film be formed and the plastic sheet of single-use bio-reactor is formed by polypropylene or tygon structure by the teflon structure example of this situation.The remainder of sensor body and electrode can be disposed on the either side of plastic sheet or sensing film.
Fig. 6 is the sketch according to the dissolved oxygen sensor that is connected with single-use bio-reactor/mixer of embodiment of the present utility model.The embodiment that shows about Fig. 6 is similar with the embodiment that shows in Fig. 3, yet main difference is the dissolved oxygen sensor that shows in Fig. 6 sensing film 80 is bonding or otherwise be attached to the plastic wall of single-use bio-reactor by flange 74.By comparison, be presented at conventional dissolved oxygen sensor 1 among Fig. 3 and have the sensing film of oneself, this sensing film is in abutting connection with the sensing film of sensor window 70 so that oxygen penetration sensor window 70 and conventional dissolved oxygen sensor 1.As shown in FIG. 6, oxygen only by sensing film 80 and basically sensed electrode reduce immediately with the generation current signal.In this way, compare the embodiment of using conventional dissolved oxygen sensor, the embodiment that is presented among Fig. 6 can be reacted a little quickly.It will be appreciated by those skilled in the art that sensor body 82 and electrode 84 do not comprise complete dissolved oxygen sensor.Therefore, for the embodiment that helps to show about Fig. 6, sensor body 82, electrode 84 and supporting electrolyte 12 are provided as kit utility basically, and described tool set can be connected with comprising or otherwise use the single-use bio-reactor that membrane support 60 as shown in FIG. 6 is connected with the sensing film.Therefore, when the single-use bio-reactor was ready to be used, sensor body 82 penetrated or otherwise is detachably fixed to membrane support 60 with electrode 84 wherein.In case link to each other, sensor body 82 and membrane support 60 form liquid-tight chamber with sensing film 80.Then thereby supporting electrolyte 12 is imported to the dissolved oxygen sensor that telotism is provided in the chamber.In case finish or cessation reaction otherwise, can from membrane support 60, take out sensor body 82 so that it is used with another single-use bio-reactor, perhaps when same bio-reactor is sterilized or otherwise accepted gamma-ray irradiation, can remove sensor body 82 from that single-use bio-reactor simply.
Although described the utility model with reference to preferred embodiment, those skilled in the art will recognize that and to make change and not deviate from spirit and scope of the present utility model with the details aspect in form.
Claims (11)
1. biological reaction container, described biological reaction container comprises:
Plastic wall; And
Oxygen sensing film, described oxygen sensing film is arranged to be close to described plastic wall, and described oxygen sensing film has known oxygen permeability.
2. biological reaction container claimed in claim 1 it is characterized in that described plastic wall comprises the hole of passing described plastic wall, and described oxygen sensing film is arranged to be close to described hole.
3. biological reaction container claimed in claim 2, it is characterized in that described biological reaction container also comprises the membrane support with the cylindrical part that passes described hole and the flange portion that is fixed to the inside surface of described plastic wall, wherein said membrane support makes described oxygen sensing film be connected with the inside surface of described plastic wall.
4. biological reaction container claimed in claim 3 is characterized in that described biological reaction container also comprises the sensor body that is connected to described cylindrical part.
5. biological reaction container claimed in claim 4 is characterized in that described sensor body is connected removably with described cylindrical part.
6. biological reaction container claimed in claim 4 is characterized in that described biological reaction container also comprises sensing electrode, and described sensing electrode is disposed in the indoor of the described sensing film of next-door neighbour.
7. biological reaction container claimed in claim 6 is characterized in that described biological reaction container also comprises analyzer, and described analyzer and described electrode operationally link to each other with based on by the dissolved oxygen DO in the current measurement biological sample of described electrode.
8. biological reaction container, described biological reaction container comprises:
Plastic wall, described plastic wall define biological respinse chamber wherein, and have the hole of passing described plastic wall;
Be fixed to the membrane support of described plastic wall, described membrane support has the cylindrical part that passes described hole; And
With the sensor fenestrated membrane that the described membrane support in the described hole of next-door neighbour is connected, described sensor fenestrated membrane has high oxygen permeability, but forms waterproof sealing with described membrane support.
9. biological reaction container claimed in claim 8 is characterized in that described biological reaction container also comprises the ammeter dissolved oxygen sensor, and described ammeter dissolved oxygen sensor is disposed in the described cylindrical part of described membrane support.
10. biological reaction container claimed in claim 9 is characterized in that described ammeter dissolved oxygen sensor penetrates described cylindrical part.
11. biological reaction container claimed in claim 10 is characterized in that described biological reaction container also comprises analyzer, described analyzer and described dissolved oxygen sensor link to each other to measure the dissolved oxygen DO of the biological sample in the described biological reaction container.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13/325,260 | 2011-12-14 | ||
| US13/325,260 US8828202B2 (en) | 2010-12-17 | 2011-12-14 | Detachable dissolved oxygen sensor for single use bioreactor/mixer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202710509U true CN202710509U (en) | 2013-01-30 |
Family
ID=47595120
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210085580.XA Active CN103163190B (en) | 2011-12-14 | 2012-03-28 | Dismountable dissolved oxygen sensor for single-use bioreactor/blender |
| CN 201220121931 Expired - Fee Related CN202710509U (en) | 2011-12-14 | 2012-03-28 | Biological reaction vessel |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210085580.XA Active CN103163190B (en) | 2011-12-14 | 2012-03-28 | Dismountable dissolved oxygen sensor for single-use bioreactor/blender |
Country Status (1)
| Country | Link |
|---|---|
| CN (2) | CN103163190B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103163190A (en) * | 2011-12-14 | 2013-06-19 | 罗斯蒙德分析公司 | Single use bioreactor/container for use with detachable dissolved oxygen sensor |
| CN107868756A (en) * | 2016-09-28 | 2018-04-03 | 罗斯蒙特分析公司 | Disposable bioreactor sensor interface |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016172507A1 (en) * | 2015-04-24 | 2016-10-27 | Rosemount Analytical Inc. | Ph sensor for single use equipment |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4263115A (en) * | 1978-07-03 | 1981-04-21 | Max Planck Gesellschaft Zur Forderung Der Wissenschaften | Ion-selective electrode device for polarographic measurement of oxygen |
| US5046496A (en) * | 1989-04-26 | 1991-09-10 | Ppg Industries, Inc. | Sensor assembly for measuring analytes in fluids |
| US6773678B2 (en) * | 2000-03-20 | 2004-08-10 | Endress + Hauser Conducta Gesellschaft Fur Mess Und Regeltechnik Mbh + Co. | Mounting system and retractable sensor holder for analytical sensors |
| GB2364125B (en) * | 2000-05-31 | 2004-07-07 | Abb Instrumentation Ltd | Bio-Sensor |
| WO2002022773A2 (en) * | 2000-09-15 | 2002-03-21 | Shell Internationale Research Maatschappij B.V. | Bioreactor |
| US6602401B1 (en) * | 2000-11-01 | 2003-08-05 | Rosemount Analytical Inc. | Amperometric sensor for low level dissolved oxygen with self-depleting sensor design |
| US20040140211A1 (en) * | 2003-01-21 | 2004-07-22 | Broy Stephen H. | Modular interface and coupling system and method |
| AU2004229070A1 (en) * | 2003-11-13 | 2005-06-02 | Dale C. Gyure | Novel bioreactor |
| US20090148941A1 (en) * | 2007-07-30 | 2009-06-11 | Peter Florez | Disposable mini-bioreactor device and method |
| US7950264B2 (en) * | 2007-11-30 | 2011-05-31 | Endress + Hauser Conducta Gesellschaft für Mess-und Regeltechnik mbH + Co. KG | Disposable measurement arrangement and method of testing and/or calibrating it |
| CN103163190B (en) * | 2011-12-14 | 2016-12-21 | 罗斯蒙德分析公司 | Dismountable dissolved oxygen sensor for single-use bioreactor/blender |
-
2012
- 2012-03-28 CN CN201210085580.XA patent/CN103163190B/en active Active
- 2012-03-28 CN CN 201220121931 patent/CN202710509U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103163190A (en) * | 2011-12-14 | 2013-06-19 | 罗斯蒙德分析公司 | Single use bioreactor/container for use with detachable dissolved oxygen sensor |
| CN103163190B (en) * | 2011-12-14 | 2016-12-21 | 罗斯蒙德分析公司 | Dismountable dissolved oxygen sensor for single-use bioreactor/blender |
| CN107868756A (en) * | 2016-09-28 | 2018-04-03 | 罗斯蒙特分析公司 | Disposable bioreactor sensor interface |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103163190B (en) | 2016-12-21 |
| CN103163190A (en) | 2013-06-19 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20190115 Address after: Minnesota, USA Patentee after: Rosemount Inc. Address before: California, USA Patentee before: Rosemount Analytical Inc |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130130 Termination date: 20210328 |