CN105921185B - A kind of urine multiple determination micro fluidic device and preparation method thereof - Google Patents
A kind of urine multiple determination micro fluidic device and preparation method thereof Download PDFInfo
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
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Abstract
The invention discloses a kind of urine multiple determination micro fluidic devices, including sample intake passage, are located at the upstream of described device；Flow channel is two or more pieces, and is connected to sample intake passage, and is located at sample intake passage downstream；Reaction zone is located in the flow channel, and including analysis test paper；Liquid collecting room is connected to positioned at the downstream of the reaction zone with the downstream end of flow channel, and the upper layer access that sample intake passage, flow channel, reaction zone and liquid collecting room are formed is made of the first passage layers and middle layer.It can be cooperated with multilayer, multiple indexes can be detected simultaneously, the process for taking urine, reaction, detection and analysis can be completed in a short time, enormously simplify operating procedure, save the time and improve efficiency.In addition, the invention also discloses the preparation method of above-mentioned apparatus, using the method for three-dimensional drawing software design path model combination 3 D-printing path model mold, can quickly, prepare to save the cost the device.
Description
Technical field
The invention belongs to technical field of biological, it is related to a kind of urine detector more particularly to a kind of urine refers to more
Mark detection micro fluidic device.
Background technique
Routine urianlysis is one of current clinically used medical inspection project, can reflect the side such as kidney and urinary system
Face the severity of disease.Certain systemic lesions and other internal organs are influenced with the disease such as diabetes, blood of change of urine
The diagnosis such as liquid disease, hepatobiliary disease, Hemorrhagic fever have important reference value.In addition, the laboratory examination of urine is to some
The therapeutic effect and Index for diagnosis of systemic disease also have important reference value.
As the improvement of people's living standards, the disease incidence of the diseases such as diabetes, gout significantly rises, people has been seriously endangered
The health of class.Currently, in most cases fast and convenient and cheap uroscopy tentatively can be carried out using urinalysis test paper,
Illness is examined with preliminary screening.The mode of operation of existing urinalysis test paper is: midstream urine is connect with urine cup, then by urinalysis
Test paper, which immerses in urine, takes urine.It is this to take urine mode comparatively laborious, and in order to guarantee to exclude other pollution effects, it is connect with urine cup
It is very inconvenient when taking midstream urine, urine be easy to splash on hand or with.In addition, what the reaction zone of urinalysis test paper was detained
Urine can be generated and be interfered with each other, and influence testing result.In addition, due to the disposable characteristic of urinalysis test paper and storage item
The requirement of part, user's preservation is improper to be easy to make the failure of urinalysis test paper to lose clinical detection meaning.Moreover, single urine
The matrix of liquid analysis test paper is softer, be easy to cause urine to drip everywhere when drawing urine.
And micro-fluidic microflow control technique is by bases such as preparation, conveying, reaction, separation and the detections of biology or chemical example
The integrated technology of this operating unit.Since the advantage of its high-throughput low cost is fast in the fields such as biomedicine, chemistry, material science
Speed development.Traditional micro-fluidic chip is mainly realized by photoetching process, needs to clean silicon wafer, spin coating, uv-exposure etc.
Series of steps, process is tedious and higher cost, accordingly, it is difficult to realize quick and inexpensive preparation.
Therefore, those skilled in the art is dedicated to developing a kind of save using simple, hygienic and convenient and being easy and can be real
The urine detection micro fluidic device that existing multi objective detects simultaneously.
Summary of the invention
In view of the above drawbacks of the prior art, simple side is used technical problem to be solved by the invention is to provide a kind of
Just it and is easy production and saves, and the urine detector of multiple indexs can be detected simultaneously.
To achieve the above object, the present invention provides a kind of urine multiple determination micro fluidic devices.
In of the invention one preferably specific embodiment, which includes:
Sample intake passage, the sample intake passage are located at the upstream of described device；
Flow channel, which is two or more pieces, and is connected to above-mentioned sample intake passage, and it is logical to be located at above-mentioned sample introduction
Road downstream；
Reaction zone, the reaction zone are located in above-mentioned flow channel, and including analysis test paper；And liquid collecting room, the liquid collecting room
Positioned at the downstream of above-mentioned reaction zone, it is connected to the downstream end of above-mentioned flow channel；
Wherein, the upper layer access that sample intake passage, flow channel, reaction zone and liquid collecting room are formed is by the first passage layers and centre
Layer is constituted.
Further, which further includes alternate path layer, and alternate path layer and middle layer constitute lower layer's access, and upper layer is logical
Road and lower layer's access are located at the two sides of middle layer.
Optionally, access is multiple, is formed by multiple first passage layers and multiple middle layers.
Further, analysis test paper is selected from detection leucocyte, nitrite, protein, occult blood, urobilinogen, bilirubin, Portugal
Grape sugar, trunk, ascorbic acid, specific gravity of urine, creatinine, microalbumin, calcium ion and pH analysis test paper.
Further, which further includes clad, and the clad is coated on the outside of above-mentioned multiple accesses, and wraps
Coating further includes funnelform injection port, and injection port is configured as a part and is connected to the entrance in channel, so that a part of liquid
Enter in sample intake passage from the entrance in channel, the chamber of a part and clad layer-forming, so that remaining liquid enters cladding
In the chamber that layer is formed.
Preferably, Bibulous Silica Gel is filled in liquid collecting room.
Another aspect of the present invention also provides a kind of preparation method of above-mentioned apparatus.
In a preferred embodiment of the invention, this method comprises:
1) three-dimensional drawing software design path model is used；
2) mold of the path model is formed by 3 D-printing；
3) it is cast on the mold using dimethyl silicone polymer, and carries out being formed by curing passage layers；
4) passage layers are demoulded, and in the reaction zone Filling Analysi test paper；
5) passage layers are subjected to corona treatment, are then assembled with the middle layer.
This method still further comprises step 6) in the passage layers and the middle layer outer cladding clad.
Further, the path model in step 1), sample intake passage width are 1~2mm, a height of 0.5~1.5mm, and flowing is logical
Road width be 0.8~1.5mm, a height of 0.8~1.5mm, reaction zone diameter be 2~5mm, a height of 1~2mm, liquid collecting room's diameter be 8~
15mm, a height of 4~10mm.
Preferably, sample intake passage width is 1.5mm, a height of 1mm, and flow channel width is 1mm, a height of 1mm, and reaction zone diameter is
4mm, a height of 1.5mm, liquid collecting room's diameter are 10mm, a height of 5mm.
Further, dimethyl silicone polymer needs first to carry out with curing agent according to weight ratio 5:1~50:1 in step 3)
Proportion mixing.Preferably, the weight ratio of dimethyl silicone polymer and curing agent is 10:1.
Further, in step 3), need to be placed in standing solidification 5~10 hours in 50~90 DEG C of baking ovens after casting.It is excellent
Selection of land stands solidification 8 hours or more in 70 DEG C of baking ovens after casting.
The present invention combines urinalysis test paper and microflow control technique, therefore urine multiple determination of the invention is micro-fluidic
Device can detect multiple indexes simultaneously, can be completed in a short time the process for taking urine, reaction, detection and analysis, enormously simplify
Operating procedure saves the time and improves efficiency.
1) after opening injection port, user, which need to only pinch device rear end cavity part, can be completed sampling and detecting step.
This micro fluidic device be omitted urine cup take urine and etc., decrease urine and splash with user, health easy to operate.
2) urine can finally enter in liquid collecting room and clad, therefore, will not be detained in flow channel and reaction zone
Diuresis liquid and cause to interfere with each other, greatly improve the accuracy of urine detection, the storage of extra urine will not be made
At secondary pollution.
3) device also guarantees that test paper will not touch outside air, can also reach good guarantor in long-term moist region
Deposit effect.
Device provided by the invention, easy to operate, detection speed is fast, and accuracy is high, detects at any time suitable for daily life
The advantages that, it is a kind of urine detection tool of Cheap highly effective, there is market application value very much.
It is described further below with reference to technical effect of the attached drawing to design of the invention, specific structure and generation, with
It is fully understood from the purpose of the present invention, feature and effect.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the urine multiple determination micro fluidic device of a preferred embodiment of the invention.
Fig. 2 is the cross-sectional view of the urine multiple determination micro fluidic device of a preferred embodiment of the invention.
Fig. 3 is the schematic diagram of the upper layer access of the urine multiple determination micro fluidic device in Fig. 2.
Fig. 4 is the instance graph of the urine multiple determination micro fluidic device of a preferred embodiment of the invention.
Fig. 5 is the use result figure of the urine multiple determination micro fluidic device in Fig. 4.
Specific embodiment
In the present invention, " upstream " used refers to that, close to the side of injection port, " downstream " refers to close to the side of liquid collecting room.
In the present invention, analysis test paper is that the test paper that uses of analysis is carried out to urine, including but not limited to detection leucocyte, nitrite,
Protein, occult blood, urobilinogen, bilirubin, glucose, trunk, ascorbic acid, specific gravity of urine, creatinine, microalbumin, calcium ion
With the urinalysis test paper such as pH.
The urine multiple determination micro fluidic device of present embodiment, including injection port, sample intake passage 1, flowing are logical
Road 2, reaction zone 3, liquid collecting room 4, analysis test paper 5.Injection port is located at the middle at whole device edge, plays sealing to device
Effect.Sample intake passage 1 and flow channel 2 are gallery, are flowed through for urine.Liquid collecting room 4 is a cavity, to collect
Urine after the reaction was completed.Analysis test paper 5 is used to carry out selective mechanisms analysis to urine.
Sample intake passage 1 is located at the downstream of injection port at the injection port of micro fluidic device.Sample intake passage 1 and flowing
Channel 2 is connected to, and flow channel 2 is located at the downstream of sample intake passage 1.Reaction zone 3 is located in flow channel 2, usually interrupts flowing
Channel 2 includes analysis test paper 5 in reaction zone 3.Flow channel 2 is also connected with liquid collecting room 4, and liquid collecting room is located at reaction zone 3 and flowing
The downstream in channel 2.
When in use, it is first turned on injection port, pinch the liquid collecting room positioned at device downstream and starts to collect urine.Usually exist
It urinates after a period of time, starts to collect midstream urine.It after urine injection port enters, flows into sample intake passage 1, and constant flow enters
In the flow channel 2 being connected with flow channel 1, then flowing is by the reaction zone 3 in flow channel 2.In reaction zone 3
In, urine can be with 5 haptoreaction of analysis test paper therein.Then, urine is flowed out from reaction zone 3, and is continued along flow channel 2
Flowing, enters finally into liquid collecting room 4.The analysis test paper 5 for passing through reaction and standard color comparison card are compared, can be completed each
The semi-quantitative analysis of item index.
Embodiment 1
The present embodiment is related to a kind of urine multiple determination micro fluidic device, as shown in Figs. 1-5 comprising upper layer access 6,
Lower layer's access 7 and middle layer 8, the device further include 8 reaction zones 3,8 indexs can be detected simultaneously, wherein 4 are distributed in
In layer access 6, in addition 4 are distributed in lower layer's access 7.Specific instance graph and use figure are as shown in Figure 4 and Figure 5.
By taking upper layer access 6 as an example, referring to Fig. 1, injection port is located at the intermediate position of the upstream edge of whole device, and sample introduction is logical
Road 1 is located at the downstream of injection port, is located substantially on the longitudinal centre line of whole device.4 21,22,23 and of first flow channel
24 are located at the two sides of sample intake passage 1, each two of every side.First flow channel 21 and 22 is arranged in parallel, 23 He of first flow channel
24 are arranged in parallel, and first flow channel 21,22 and first flow channel 23,24 are interspersed, i.e., in first flow channel
21 and 23,22 and 24 are not on an axis in the transverse direction of whole device.
In another specific embodiment, flow channel can also be such as 2 in sample intake passage side with mal-distribution,
The other side is 3, or is entirely located in the side of sample intake passage.
Reaction zone 31,32,33 and 34 is located in first flow channel 21,22,23 and 24.31,32,33 and of reaction zone
34 be a border circular areas, and the central point of each of which reaction zone is respectively positioned on the axis of corresponding flow channel.5 quilt of analysis test paper
It places in the reaction region, also, each analysis test paper is different, to detect different indexs.
In another embodiment, reaction zone can also have other shapes, as long as analysis test paper 5 can be accommodated.Separately
Outside, reaction zone is also not necessarily located on the axis of flow channel, as long as the liquid in flow channel can flow into reaction zone i.e.
It can.
In reaction zone downstream, two first flow channels 21 and 22 positioned at 1 side of sample intake passage merge into one second
Flow channel 25, it is logical that two first flow channels 23 and 24 positioned at 1 other side of sample intake passage merge into another second flowing
Road 26, two second flow paths are merged into a third flow channel 27 again.The axis of third flow channel 27 substantially and dress
The longitudinal centre line set is coaxial.
Third flow channel 27 is connected with the liquid collecting room 4 locateding downstream.It is first-class in another specific embodiment
Dynamic channel can also individually can be connected with liquid collecting room 4 in the downstream of reaction zone with nonjoinder；Or second flow path
It can be individually connected with liquid collecting room 4 with nonjoinder.
Lower layer's access 7 is consistent with the arrangement of upper layer access 6, and details are not described herein.
Middle layer 8 is configured between upper layer access 6 and lower layer's access 7, as shown in Figure 2.Middle layer 8 is by upper layer access under
Layer passage portion separates, and upper layer access 6 and lower layer's access 7 are substantially symmetric.Wherein, middle layer 8 is by upper layer access 6 and lower layer's access
7 channel part is completely separated, in order to avoid causing cross influence when detection, reduces accuracy in detection.But it is right in middle layer 8
It should be provided with hole in middle layer 8 in the part of liquid collecting room 4, upper layer access 6 is connected to the liquid collecting room 4 of lower layer access 7, from
And increase the volume that injection port draws liquid.Hole in middle layer 8 can be one or more.
The device of the present embodiment is sealing before not opening use, ensure that the isolation of test paper and external environment.
In another embodiment, it in upper layer access 6, the outside of middle layer 8 and lower layer's access 7, can be coated by clad,
Further provide the internal environment of one with external environs.
Embodiment 2
The present embodiment is related to another urine multiple determination micro fluidic device comprising by the first upper layer access, first
The first unit of lower layer's access and the first middle layer composition, by the second upper layer access, second lower layer's access and the second middle layer group
At second unit.First unit and second unit are relatively independent, and their internal structure is similar, respectively with embodiment 1
In internal structure it is similar.Clad is coated on the outside of two units, make first unit and second unit and external environment every
From, while clad itself also forms a bag-like container.
In the inside of clad, close to the inlet of the sample intake passage of two units, being provided with to pull open to form funnel-form
Injection port, a part of the bottom of the injection port is connected to the feeder connection of two units, allow a part of liquid into
Enter funnel-form injection port, then smoothly flows into sample intake passage, the chamber of a part and clad layer-forming, so that remaining liquid
Into in the chamber of clad layer-forming.
In the urine multiple determination micro fluidic device of the present embodiment, realize while the detection to more multi objective.
Embodiment 3
The present embodiment is related to a kind of urine multiple determination micro fluidic device preparation method as in embodiment 1, specifically such as
Under:
1, the design of 3D mold: use three-dimensional drawing software design path model, wherein 1 width of sample intake passage be 1.5mm,
A height of 1mm, 2 width of flow channel be 1mm, a height of 1mm, reaction zone diameter 4mm, a height of 1.5mm, 4 diameter of liquid collecting room be 10mm,
A height of 5mm.
2, print die: using fusion sediment formula accumulation technology and hot-melt plastic, before printing is formed on a substrate
State 3D mold.
3, it is poured: PDMS (dimethyl silicone polymer) and curing agent being mixed according to weight ratio 10:1, and stirred and evenly mixed, is poured
Note is placed in 70 DEG C of baking ovens on mold and stands solidification 8 hours or more.Wherein, curing agent is curing agent commonly used in the art, packet
Include Sulfur, selenium, tellurium, sulfur-containing compound, metal oxide, peroxide, resin, quinones and amine etc..
4, demould: by after solidification PDMS demould, formed passage layers, and correspond to reaction zone position fill it is different
Analysis test paper.
5, it assembles: corona treatment being carried out to the passage layers of formation, makes its surface active, then by two paths layer
(i.e. upper layer passage layers and lower layer's passage layers) are assembled with middle layer by being bonded, as shown in Figure 3.Wherein, middle layer is one
Layer PDMS has multiple holes in the position for corresponding to liquid collecting room；Corona treatment is that channel layer is put into plasma surface
It in active instrument, is evacuated to form vacuum tightness environment, RF Level is adjusted to HI grades, timing 1-2min is with 1.5min
It is good, to realize surface active.
Embodiment 4
The present embodiment is related to a kind of urine multiple determination micro fluidic device preparation method, specific as follows:
1, the design of 3D mold: three-dimensional drawing software design path model is used, wherein 1 width of sample intake passage is 2mm, height
For 1.5mm, 2 width of flow channel is 1.5mm, a height of 1.5mm, reaction zone diameter 5mm, a height of 2mm, and 4 diameter of liquid collecting room is
15mm, a height of 10mm.
2, print die: using fusion sediment formula accumulation technology and hot-melt plastic, before printing is formed on a substrate
State 3D mold.
3, it is poured: PDMS and curing agent being mixed according to weight ratio 50:1, and stirred and evenly mixed, is cast on mold, is placed in
Solidification 10 hours are stood in 90 DEG C of baking ovens.
4, demould: by after solidification PDMS demould, formed passage layers, and correspond to reaction zone position fill it is different
Analysis test paper.
5, it assembles: corona treatment being carried out to the passage layers of formation, makes its surface active, then by two paths layer
(i.e. upper layer passage layers and lower layer's passage layers) are assembled with middle layer by being bonded.Wherein, middle layer is one layer of PDMS, right
There should be multiple holes in the position of liquid collecting room；Corona treatment is put into channel layer in plasma surface activity instrument, is taken out
RF Level is adjusted to HI grades, timing 1.5min, to realize surface active to vacuum tightness environment is formed by vacuum.
6, it coats: after channel layer and middle layer are completed, a clad is coated outside it.
Embodiment 5
The present embodiment is related to a kind of urine multiple determination micro fluidic device preparation method, specific as follows:
1, the design of 3D mold: three-dimensional drawing software design path model is used, wherein 1 width of sample intake passage is 1mm, height
For 0.8mm, 2 width of flow channel is 0.5mm, high 0.8mm, reaction zone diameter 2mm, a height of 1mm, 4 diameter of liquid collecting room be 8mm,
A height of 4mm.
2, print die: using fusion sediment formula accumulation technology and hot-melt plastic, before printing is formed on a substrate
State 3D mold.
3, it is poured: PDMS and curing agent being mixed according to weight ratio 5:1, and stirred and evenly mixed, is cast on mold, is placed in 50
Solidification 5 hours are stood in DEG C baking oven.
4, demould: by after solidification PDMS demould, formed passage layers, and correspond to reaction zone position fill it is different
Analysis test paper.
5, it assembles: corona treatment being carried out to the channel layer of formation, makes its surface active, then by two paths layer
(i.e. upper layer passage layers and lower layer's passage layers) are assembled with middle layer by being bonded.Wherein, middle layer is one layer of PDMS, right
There should be multiple holes in the position of liquid collecting room；Corona treatment is put into channel layer in plasma surface activity instrument, is taken out
RF Level is adjusted to HI grades, timing 1.5min, to realize surface active to vacuum tightness environment is formed by vacuum.
6, it coats: after passage layers and middle layer are completed, a clad is coated outside it.
Embodiment 6
The present embodiment is related to a kind of urine multiple determination micro fluidic device preparation, and structure is similar in embodiment 1.
But in the device of this implementation, a certain amount of Bibulous Silica Gel is filled in liquid collecting room.The Bibulous Silica Gel is in the logical of the device
Road is placed into device together with analysis test paper after casting demoulding.
On the one hand Bibulous Silica Gel can keep the device to maintain dry state during storage, so that analysis test paper is protected
More good state is held, to guarantee the accuracy of subsequent analysis.On the other hand, when using the device, Bibulous Silica Gel contacts
To liquid, and liquid is absorbed, to generate the effect of drainage to the liquid in channel, liquid can be made smoothly logical in flowing
It is flowed in road.In another aspect, absorption of the Bibulous Silica Gel to liquid, can prevent liquid holdup in reaction zone, avoid tying to measurement
Fruit has an impact.
The preferred embodiment of the present invention has been described in detail above.It should be appreciated that the ordinary skill of this field is without wound
The property made labour, which according to the present invention can conceive, makes many modifications and variations.Therefore, all technician in the art
Pass through the available technology of logical analysis, reasoning, or a limited experiment on the basis of existing technology under this invention's idea
Scheme, all should be within the scope of protection determined by the claims.
Claims (4)
1. a kind of urine multiple determination micro fluidic device, which is characterized in that including
Sample intake passage, the sample intake passage are located at the upstream of described device；
Flow channel, the flow channel are two or more pieces, are arranged in parallel, are connected to the sample intake passage, and are located at described
Sample intake passage downstream；
Reaction zone, the reaction zone are located in the flow channel, and including analysis test paper；And
Liquid collecting room, the liquid collecting room are located at the downstream of the reaction zone, are connected to the downstream end of the flow channel；
Wherein, the upper layer access that the sample intake passage, the flow channel, the reaction zone and the liquid collecting room are formed is by first
Passage layers and middle layer are constituted；The upper layer access be it is multiple, by multiple first passage layers and multiple middle layer shapes
At；Described device further includes clad, and the clad is coated on the outside of the multiple upper layer access, and the packet
Coating further includes funnelform injection port, and the injection port is configured as a part and is connected to the entrance of the sample intake passage, makes
It obtains a part of liquid to enter in the sample intake passage from the entrance of the sample intake passage, a part of chamber with the clad layer-forming
Room connection, so that remaining liquid enters in the chamber of the clad layer-forming；Bibulous Silica Gel is filled in the liquid collecting room；It is described
First passage layers are demoulded by the PDMS after solidifying and are formed；The middle layer is one layer of PDMS, and it is white that the analysis test paper is selected from detection
Cell, nitrite, protein, occult blood, urobilinogen, bilirubin, glucose, trunk, ascorbic acid, specific gravity of urine, creatinine, calcium from
The analysis test paper of son and pH；
The preparation method of the liquid multiple determination micro fluidic device the following steps are included:
1) three-dimensional drawing software design path model is used；
2) mold of the path model of the step 1) design is formed by 3 D-printing；
3) it is cast on the mold using dimethyl silicone polymer, and carries out being formed by curing passage layers；
4) passage layers are demoulded, and in the reaction zone Filling Analysi test paper；
5) passage layers are subjected to corona treatment, are then assembled with the middle layer,
Wherein, the path model in step 1), sample intake passage width are 1~2mm, a height of 0.8~1.5mm, and flow channel is wide
For 0.5~1.5mm, a height of 0.8~1.5mm, reaction zone diameter is 2~5mm, a height of 1~2mm, liquid collecting room's diameter is 8~
15mm, a height of 4~10mm.
2. urine multiple determination micro fluidic device as described in claim 1, which is characterized in that described device further includes second
Passage layers, the alternate path layer and middle layer constitute lower layer's access, and the upper layer access and lower layer's access are located at described
The two sides of middle layer.
3. a kind of device as described in claim 1, which is characterized in that dimethyl silicone polymer described in step 3) need first and
Curing agent carries out proportion mixing according to weight ratio 5:1~50:1.
4. a kind of device as described in claim 1, which is characterized in that in step 3), need to be placed in 50~90 DEG C after casting
Solidification 5~10 hours are stood in baking oven.
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| CN110736776A (en) * | 2019-09-25 | 2020-01-31 | 山东大学 | urine detection electrochemical sensor, paper diaper and preparation method |
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| CN107807162A (en) * | 2017-09-28 | 2018-03-16 | 珠海沃姆电子有限公司 | Urine parameters detection means and urine parameters monitoring system |
| CN107824232B (en) * | 2017-10-25 | 2019-12-20 | 中国科学院电子学研究所 | Microfluidic chip for creatinine detection, preparation method thereof and creatinine detection method |
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| CN103041876A (en) * | 2012-12-27 | 2013-04-17 | 济南大学 | Preparation of electrochemical three-dimensional microfluidic paper chip with high flux, low cost and simplicity in operation, and application of electrochemical three-dimensional microfluidic paper chip to field test |
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| CN102671729A (en) * | 2012-05-07 | 2012-09-19 | 博奥生物有限公司 | Micro-fluidic chip for multi-index biochemical detection |
| CN103041876A (en) * | 2012-12-27 | 2013-04-17 | 济南大学 | Preparation of electrochemical three-dimensional microfluidic paper chip with high flux, low cost and simplicity in operation, and application of electrochemical three-dimensional microfluidic paper chip to field test |
| CN104965082A (en) * | 2015-05-21 | 2015-10-07 | 大连理工大学 | Micro-fluidic chip used for simultaneous detection of bladder cancer cells, calculi, blood cells, and bacteria in urine |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110736776A (en) * | 2019-09-25 | 2020-01-31 | 山东大学 | urine detection electrochemical sensor, paper diaper and preparation method |
| CN110736776B (en) * | 2019-09-25 | 2020-10-13 | 山东大学 | Urine detection electrochemical sensor, paper diaper and preparation method |
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