CN206756858U - A kind of liquid sample guiding device, detection strip and detecting instrument - Google Patents
A kind of liquid sample guiding device, detection strip and detecting instrument Download PDFInfo
- Publication number
- CN206756858U CN206756858U CN201621113883.8U CN201621113883U CN206756858U CN 206756858 U CN206756858 U CN 206756858U CN 201621113883 U CN201621113883 U CN 201621113883U CN 206756858 U CN206756858 U CN 206756858U
- Authority
- CN
- China
- Prior art keywords
- sample
- detection strip
- channel plate
- guiding device
- strip
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/66—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood sugars, e.g. galactose
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Diabetes (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The utility model discloses a kind of liquid sample guiding device, detection strip and detecting instrument, the guiding device to include cover plate and channel plate, and one end is provided with sample holes on the cover plate, and the other end is provided with gas outlet；The channel plate is provided with sample cavity, fluid flow paths and opening, wherein, sample cavity is located at the lower section of the sample holes, and connect opening by fluid flow paths, opening is extended with extensional pipeline relative to the distal end of sample cavity in the channel plate, when cover plate closed assembly is on channel plate, channel plate limits a ventilation mouth communicated with the external world by gas outlet, the ventilation mouth is located on any point of the extensional pipeline, or the more far-end positioned at the extensional pipeline end relative to the sample cavity；The detection strip is formed by above-mentioned guiding device, substrate, above-mentioned cover plate and channel plate closed assembly；In addition, also disclose the detecting instrument containing above-mentioned detection strip.
Description
Technical field
A kind of instant detection means of blood is the utility model is related to, in particular it relates to a kind of blood guiding device and detection
Strip, and the detecting instrument containing the strip.
Background technology
Immediately detection (POCT, point-of-care testing) refers to the rapid clinical detection carried out beside patient,
Clinical examination teacher is generally not necessarily to carry out, but can be completed by nurse, doctor or even patient oneself.This is that one kind exists
Sampling location is analyzed at once, is saved complex process program of the sample in laboratory inspection, is quickly obtained assay
A kind of new method.By definition, POCT is to be carried out in sampling location, be quick using portable analysers and matched reagent
Obtain a kind of detection mode of testing result.
In recent years, POCT is widely used, including each section office of hospital, basic hospital, community clinic and family, such as
Vim and vigour, electrolyte balance, the instant detection of Applications of Cardiac Markers are progressively widely used in emergency ward, ICU and operating room；Family expenses blood
Sugared instrument widely uses the most successful example of even more POCT.
Another example is prothrombin time PT/INR quick detection, and INR-POCT systems provide one kind for patient
Simple, fast and easy, safe method monitoring INR values, take finger tip blood examination to survey, easily standardization simple to operate, 1 minute or so
Accurate testing result is can obtain, avoids from collection of specimens, be transported to the delay of detection.Existing INR-POCT systems are more
It is made up of an electrochemical analyser and disposable detection strip.
The utility model is applied to POCT detection strips, and it cannot be only used for the quick detection of blood, it can also be used to its
Its fluid sample such as urine.Chinese patent CN2788184Y discloses a kind of biological testing bar, and it is related to blood in detection whole blood
A kind of biological testing bar of ketone body concentration or concentration of glucose, it includes：The substrate of electrode is loaded with, is provided with reaction reagent
Reaction zone space, hydrophilic cover, the wall between the substrate and hydrophilic cover；Wherein, the electrode includes working electrode
And reference electrode；There is the groove of openings at one side on the wall；The reaction zone space is by having the fluted interval
Layer limits to be formed with the substrate, hydrophilic cover, and has sample holes and steam vent.Described steam vent is located at the hydrophilic lid
On plate.The utility model be clinically provide it is a kind of can the biological testing bar that increases of quick detection blood ketone body concentration, not only
Cost is low, simple in construction, and easy to operate, can carry out the quick detection of DKA.The knot of the biological testing bar
Structure is the usual way of existing coherent detection strip.However, because cover plate gas outlet is arranged on the surface of reaction chamber, cause sample
Turbulent flow and bubble are also easy to produce when this is flowed to during reaction chamber from sample cavity, so as to influence the repeatability of testing result.
Utility model content
One of the purpose of this utility model is to provide a kind of liquid sample guiding device, and it has more under atmospheric environment
Good liquid guide flow function.Further, there is provided a kind of liquid sample detection strip comprising the liquid sample guiding device.More
Further, there is provided a kind of detecting instrument for including aforesaid liquid pattern detection strip so that reliable during progress POCT detections
Property and repeatability are improved.
To solve above-mentioned first purpose, technical scheme is as follows used by the utility model：
A kind of liquid sample guiding device, including cover plate and the overlappable channel plate below cover plate, one on the cover plate
End is provided with sample holes, and the other end is provided with gas outlet；The channel plate is provided with sample cavity, fluid flow paths and opening, wherein,
When the cover plate closed assembly is on the channel plate, the sample cavity is located at the lower section of the sample holes, and passes through the liquid
Body circulation passage connects the opening, and the opening of the channel plate is also associated with extensional pipeline, and its bearing of trend is
Relative to the other side of the sample cavity and the liquid communication pipeline, when the cover plate closed assembly is on channel plate, institute
State channel plate and one ventilation mouth communicated with the external world is limited by gas outlet, the ventilation mouth is located at appointing for the extensional pipeline
Anticipate on a bit, or the ventilation mouth at least partly contacts with the extensional pipeline.
It should be noted that liquid sample can flow into sample cavity from sample holes, then liquid passes through fluid flow paths
It flow to opening.Channel plate limits a ventilation mouth communicated with the external world by the gas outlet of cover plate one end, for liquid flow
Liquid in circulation passage is breathed freely with the external world.The ventilation mouth can be located on the optional position of extensional pipeline, include the end of extensional pipeline
End, or ventilation mouth at least partly contact with extensional pipeline.It is logical in extension as one of which preferred embodiment, ventilation mouth
The end in road.
Preferably, ventilation mouth does not overlap with the opening.
It should be noted that ventilation mouth is located in above-mentioned extensional pipeline any position (rather than located immediately at conversion zone
On), it is that a segment distance (such as 1mm -10mm) is formed between ventilation mouth and described conversion zone in particular, may be such that liquid
Can more rapidly, uniformly, reliably full of conversion zone.
Preferably, a diameter of 0.1mm -5mm of the gas outlet.
Preferably, the cross-sectional area of described extensional pipeline is relative to the cross-sectional area ratio of the fluid flow paths
1:1–1:10。
Preferably, the length ratio of the length of the extensional pipeline and the fluid flow paths is 1:1–1:10.
Preferably, the tube wall of the fluid flow paths at least partially water wetted material, and the water wetted material position
Through the both ends of fluid connection passage, liquid is thus more beneficial for after sample holes contact guiding device, is led to along fluid connection
Road flows to opening and therewith into the conversion zone on the substrate of lower section.
It should be noted that by the way that the liquid communication pipeline is at least partly made with water wetted material, band is enabled to
The molecular line liquid sample of polarized group flows toward liquid communication pipe end direction；Preferably implement as one of which
Mode, at least wherein one whole section of the tube wall from the liquid communication pipeline head end to end is water wetted material, may be such that liquid
Sample preferably flows from sample cavity toward opening direction.As a more preferred embodiment, the liquid flow siphunculus
The all water wetted materials of tube wall in road, liquid flowing is better under this embodiment.
Preferably, the tube wall of the extensional pipeline at least partially hydrophobic material；It is thin to be preferably close to air outlet parts
Water material, and be water wetted material close to circulation bore portion, the length of water wetted material part is preferably formed with 1-2 mm.
It should be noted that the tube wall of the extensional pipeline is made with hydrophobic material at least in part, can prevent
Liquid sample is lost in caused by liquid sample flows into extensional pipeline；As one of which preferred embodiment, prolong described in
It is hydrophobic material to stretch tube wall at least wherein one whole section of the pipeline head end to end, can preferably prevent liquid sample from liquid flow
Thread a pipe and flowed toward extensional pipeline direction；As a more preferred embodiment, the tube wall of the extensional pipeline is all
Hydrophobic material, liquid is prevented to flow under this embodiment better.
Preferably, the sample cavity, fluid flow paths, opening, extensional pipeline and ventilation mouth are respectively positioned on same straight line
On.
Wherein, when the sample cavity, fluid flow paths, opening, extensional pipeline and ventilation mouth are respectively positioned on same straight line
When upper, on the one hand, liquid sample more can be flowed into promptly in opening from sample cavity, improve flow efficiency；On the other hand, flow
Through hole forms straight-through ventilative with ventilation mouth, it is possible to increase air permeable effect, is that the flowing of liquid improves efficiency.
Preferably, the sample cavity is located at the underface of the sample holes.
It should be noted that when sample cavity is located at the underface of the sample holes, liquid can be unhinderedly from sample holes
Flow into sample cavity.
Preferably, it is characterised in that distance of the described ventilation mouth away from the conversion zone end is 1mm -10 mm.
Preferably, the area of the ventilation mouth and the area ratio of the sample holes are 1:1–1:100.
Preferably, a diameter of 0.3mm -5mm of described ground ventilation mouth.
To realize above-mentioned second purpose, technical scheme is as follows used by the utility model：
A kind of liquid sample detects strip, and containing above-mentioned guiding device, the strip also contains substrate, on the substrate
Provided with conversion zone, when the substrate closed assembly is under the channel plate, the opening is located on the conversion zone
It is and least partially overlapped with it.
Wherein, the opening is used to accept liquid sample so that liquid sample enters reaction zone by the opening
Domain, the conversion zone provide reaction compartment for liquid sample.
It should be noted that the mounting means as one of which liquid sample detection strip, substrate, channel plate and cover plate
Closed assembly successively from the bottom to top.
Preferably, described fluid flow paths are groove, and the groove can be with right and wrong hollow out or hollow out, when described
Groove for hollow out when, when the upper and lower surface of channel plate distinguishes closed assembly cover plate and substrate, limited jointly by them Lai
Space forms circulation duct, and a side wall of pipeline is cover plate lower surface, and another side wall is upper surface of base plate.
It should be noted that fluid flow paths have a variety of enforceable modes.For the technical program, liquid communication
When passage can distinguish closed assembly cover plate and substrate by the upper and lower surface of groove or channel plate, limited jointly by them Lai
The pipeline that space is formed is realized.As one of which embodiment, the lower section of the fluid flow paths part on channel plate is simultaneously
Not hollow out, but the liquid flow groove to lower recess is formed, liquid is flowed into conversion zone by groove；Implement as another kind
Mode, when fluid flow paths have been upper and lower surface difference closed assembly cover plate and the substrate of channel plate, and limited jointly by them
During the pipeline that the space come is formed, fluid flow paths part on channel plate hollow out up and down, fluid flow paths by cover plate and
The common closed assembly of substrate simultaneously limits gained, and liquid is flowed into conversion zone by the restriction passage.
Preferably, the conversion zone includes water wetted material.
When the conversion zone includes water wetted material, liquid sample can be attracted to flow into conversion zone from sample cavity so that
Liquid sample flowing is more rapid, realizes react into conversion zone more quickly.
It is further preferred that the tube wall of the circulation duct at least partially water wetted material, and the water wetted material portion
The both ends of fluid connection passage are run through in position.
No matter the liquid communication pipeline is to limit to be formed by above-mentioned closed assembly in a manner of which kind of, in general embodiment,
The fluid flow paths have tube wall (such as two side walls of hollow out groove in itself and the lid that can be substantially expressed as on four direction
Plate, the part of substrate and the hollow out groove restriction, or be that non-hollow out groove in itself with described non-engrave by three tube walls and cover plate
The part of empty groove restriction).As one of which preferred embodiment, four tube walls at least one be with hydrophilic material
What material made, it may be such that liquid preferably flows from the sample cavity toward the opening direction.When whole four tube walls
When being made of water wetted material, aforesaid liquid flow effect is more preferably.
On the basis of above-mentioned embodiment, the embodiment being more highly preferred to is the extensional pipeline bag of the strip
Containing hydrophobic material, or partly it is made by hydrophobic material.
So, hydrophilic segment applies pulling force so as to pull sample to flow forward to sample liquids.When sample passes through the stream
During through hole, its hydrophobic effect of the extensional pipeline can prevent sample from continuing to flow.
Preferably, the hydrophobic material is plastics, can be specifically PET either ABS or PC etc., as wherein one
Kind preferred embodiment, the water wetted material can be by carrying out hydrophilic treated come real on the hydrophobic material to its surface
It is existing, such as physical treatment, chemical treatment, sided corona treatment or other processing methods.Preferably, described cover plate closed assembly is in channel plate
On, the closed assembly is movable closed assembly or fixed closed assembly；Or described substrate closed assembly is somebody's turn to do under channel plate
Closed assembly is movable closed assembly or fixed closed assembly.
The closed assembly between substrate and channel plate, can be movable closed assembly it should be noted that between cover plate and channel plate
Or fixed closed assembly, need to select closed assembly mode, the progress of convenient test according to test-types.The closed assembly can pass through
Various methods are realized, for example the objects such as buckle or screw can be used to bind together the part；Or it can pass through
Relevant part is sticked together using double faced adhesive tape；Or can be by heating (such as ultrasonic wave) by each parts surface portion
Divide fusing and then be sticked together.
Preferably, electrode is provided with described conversion zone；Preferably, the conversion zone is also loaded with reaction examination in advance
Agent；It is highly preferred that the reaction reagent is dry reagent or passes through pretreatment into dry reagent.
It should be noted that electrode can be provided with according to being actually needed for detection in conversion zone so that liquid flows into anti-
Answer when being contacted in region with the electrode, energization can be reacted.Further, can be preset in conversion zone required
Reaction reagent so that liquid is flowed into the conversion zone can be reacted when being contacted with reaction reagent.Reaction reagent is preferred
Be dry reagent or pretreatment into dry reagent, prevent reaction reagent from being overflowed in the storage process of detection strip.
Preferably, one padded is additionally provided between described channel plate and the substrate, in the conversion zone
Side limits the conversion zone wall body increased.
It should be noted that it can be that testing liquid and reaction reagent carry out reaction and carried to limit the conversion zone wall body increased
For certain reaction compartment, to improve the effect reacted and detected.As a kind of preferred scheme, padded is that double faced adhesive tape is padded
Piece, the fixation of padded is better achieved, prevents the dislocation of padded from moving, prevent testing liquid from can not smoothly flow into reaction zone
In domain.
Preferably, described detection strip is blood testing strip；It is highly preferred that detect strip for prothrombin time.
It should be noted that prothrombin time detection strip can be prothrombin time (PT), activated partial blood coagulation
Movable enzyme time (aPTT), and other blood parameters detection strip, such as blood sugar test strip.
To realize above-mentioned 3rd purpose, technical scheme is as follows used by the utility model：
A kind of detecting instrument containing above-mentioned detection strip, also containing reaction signal collecting device or signal processing and analysis
Equipment, for detection strip conversion zone reacted physics, chemistry or electric signal to be conducted to signal collecting device, or conduction
To signal processing and analysis equipment and be further analyzed analysis and result show.
Preferably, described detecting instrument is factor detector.
Compared with prior art, the beneficial effects of the utility model are：
1st, communicated by the fluid flow paths on the gas outlet and channel plate on cover plate and extension passage composition with the external world
Ventilation mouth, ventilation mouth is located at extensional pipeline or the more far-end located at extensional pipeline end directly over the opening,
It may be such that blood sample to be measured is evenly more reliably full of conversion zone from sample cavity through fluid course, to improve the weight of test result
Renaturation and reliability.
2nd, by setting fluid flow paths and extension passage, can avoid during liquid fills conversion zone anti-
Region is answered to produce bubble, in order to avoid influence reaction effect.
3rd, preferable extensional pipeline cross-sectional area, fluid course cross-sectional area, distance of the ventilation mouth away from conversion zone with
And under ventilative open area, it can more preferably realize liquid flow effect.
4th, by setting padded between channel plate and substrate so that limit the reaction increased above conversion zone
Region wall body can provide bigger reaction compartment for the reaction of blood sample to be measured and reaction reagent, ensure good reaction effect.
Described above is only the general introduction of technical solutions of the utility model, in order to better understand skill of the present utility model
Art means, and being practiced according to the content of specification, and in order to allow above and other purpose of the present utility model, feature
It can be become apparent with advantage, below especially exemplified by preferred embodiment, and coordinate accompanying drawing, described in detail as follows.
Brief description of the drawings
Fig. 1 is the biological testing bar structural representation of prior art；
Fig. 2 is the structural representation for the first structure that the utility model is applied to detection strip；
Fig. 3 is the structural representation for second of structure that the utility model is applied to detection strip；
Fig. 4 is the structural representation for the third structure that the utility model is applied to detection strip；
Fig. 5 is that the fluid flow paths of the utility model detection strip are the left view of groove；
Fig. 6 is the water conservancy diversion design sketch that experiment Distribution Test prior art detects strip A；
Fig. 7 is the water conservancy diversion design sketch that experiment Distribution Test the utility model detects strip B；
In figure, 1, cover plate；11st, sample holes；12nd, gas outlet；2nd, channel plate；21st, sample cavity；22nd, fluid flow paths；
23rd, opening；24th, extensional pipeline；3rd, substrate；31st, conversion zone；32nd, electrode；4th, padded；41st, padded through hole.
Embodiment
Further to illustrate that the utility model is to reach the technological means and effect that predetermined purpose of utility model taken,
Below in conjunction with accompanying drawing and preferred embodiment, to according to specific embodiment of the present utility model, structure, feature and its effect, in detail
Carefully it is described as follows：
The embodiment of prior art
Fig. 1 is the structural representation of prior art.The biological testing bar includes：The substrate 101 of electrode is loaded with, is provided with
Double faced adhesive tape between the reaction zone space of reaction reagent 104 and hydrophilic cover 109, in addition to substrate 101 and hydrophilic cover 109
Wall 105, the groove 108 formed with openings at one side on double faced adhesive tape wall 105, with the substrate 101 and hydrophilic cover
109, which limit formation one, has the affiliated reaction zone space of sample holes 401 and steam vent 107；Sample holes 401 are located at double faced adhesive tape on one side
Opening 106 at, steam vent 107 is located on hydrophilic cover 109.Reaction zone space is arranged on one end of substrate 101, so can be with
Directly from the finger of tester or other position blood-sample withdrawals from injection port 401 to the reaction zone space.Electrode includes work electricity
Pole 102 and reference electrode 103, testing liquid enter described reaction zone space through sample holes 401, entered with reaction reagent 104
Row reaction, electric signal caused by reaction are exported by working electrode and reference electrode through the connecting wire of electrode connection terminal 110.
Embodiment one
Fig. 2 is the structural representation of the first embodiment of the utility model, i.e. the knot for liquid sample detection strip
Structure.The composed component of liquid sample detection strip includes cover plate 1, channel plate 2 and substrate 3.Wherein, sample holes 11 are located at cover plate 1
Left end, gas outlet 12 be located at cover plate 1 right-hand member.Channel plate 2 be provided with sample cavity 21, the groove as fluid flow paths 22 and
Opening 23.Groove is hollow out, when the upper and lower surface of channel plate distinguishes closed assembly cover plate 1 and substrate 3, by their common limits
The space fixed forms circulation duct, and a side wall of pipeline is cover plate lower surface, and another side wall is the upper surface of substrate 3.Its
In, sample cavity 21 is located at the left end of channel plate 2, and when the closed assembly of cover plate 1 is on channel plate 2, sample cavity 21 is located at cover plate 1
In sample holes 11 underface.
In addition, sample cavity 21 and opening 23 are connected by fluid flow paths 22 so that liquid can be through liquid communication
Passage 22 is flowed into opening 23 from sample cavity 21.Opening 23 is extended with fluid flow paths relative to the distal end of sample cavity 21
22 extensional pipeline 24.Gas outlet 12 on cover plate 1 is located at the surface of the end of extensional pipeline 24, is limited with channel plate 2
Ventilation mouth (not marked in figure) is located at the end of extensional pipeline 24 so that extensional pipeline 24 by gas outlet 12 by being limited
Ventilation mouth is in communication with the outside.
In the present embodiment, fluid flow paths 22 are the passage of about one hollow out, specifically channel plate 2 is upper
When lower surface difference closed assembly cover plate 1 and substrate 3, limited jointly by them Lai the pipeline that is formed of space.
Substrate 3 is loaded with conversion zone 31 and electrode 32, has dry reaction reagent (not marked in figure) on conversion zone 31.
Conversion zone 31 is located at the underface of the opening 23 of channel plate 2.
Set cover plate 1, channel plate 2 and substrate 3 is by way of movable closed assembly as described above for part, from the bottom to top successively
Closed assembly, form liquid sample detection strip.
Conversion zone 31, which is more quickly flowed into, for guiding fluid sample realizes reaction, it is in preferred embodiments, described
Conversion zone 31 includes water wetted material, or the conversion zone 31 is made by water wetted material at least in part.
And in highly preferred embodiment, four tube wall (such as hollow out ditches possessed by the fluid flow paths 22
Two side walls of groove in itself and cover plate 1, substrate 3 and the part of the hollow out groove restriction, or be non-hollow out groove in itself three
Individual tube wall and cover plate 1 and the part of the non-hollow out groove restriction) at least one tube wall is made by water wetted material；
Certainly another alternative embodiment, at least described liquid communication pipeline include water wetted material, or portion at least in part
Ground is divided to be made by water wetted material；When four tube walls are all made up of water wetted material, the effect above is more preferably.
And in order to prevent liquid sample from flowing out the gas outlet 12 from the extensional pipeline 24, the quality of reaction is influenceed,
In preferred embodiment, the extensional pipeline 24 includes hydrophobic material, or is made up at least in part of hydrophobic material；Lean on
Nearly air outlet parts are hydrophobic material, and are water wetted material close to circulation bore portion.
In use, clicking and entering testing liquid sample in sample holes 11, liquid sample flows into sample cavity 21 from sample holes 11, covered
In the presence of the water wetted material of plate 1, liquid sample flows into conversion zone 31 through fluid flow paths 22, in the process, due to prolonging
The ventilation mouth for stretching the end of pipeline 24 is in communication with the outside by the gas outlet 12 on cover plate 1 so that liquid can be in liquid flow all
Flowed forward in road 22.After testing liquid sample is full of conversion zone 31, extensional pipeline 24 will be then flowed into forward, directly
Hydrophobic part to extensional pipeline 24 prevents to flow by its hydrophobic effect.If the viscosity of fluid sample is larger, fluid sample has
Flowing may just be stopped before the hydrophobic part of extensional pipeline 24 is not reached, because now being produced by the hydrophilic interaction of cover plate 1
Pulling force and the flow resistance of fluid sample reach balance.By in liquid sample detection strip insertion detecting instrument so that electrode
32 are powered.Now, liquid sample, reaction reagent are reacted in the electrode 32 of energization.
Embodiment two
Fig. 3 is the structural representation of second of embodiment of the utility model, and present embodiment is in above-described embodiment one
On the basis of add one padded 4, be similarly for liquid sample detection strip structure.Specifically, the pad
The radial dimension of the padded through hole 41 of high piece 4 thereon and the phase of conversion zone 31 on the opening 23 and substrate 3 on channel plate 2
Together.By way of stickup, it is fixed between channel plate 2 and substrate 3 for padded 4, and causes opening 23, padded through hole
41 and conversion zone 31 be located on same vertical plane.In preferred embodiments, except conversion zone 31 is made by water wetted material
Outside, the remainder of substrate 3 is made by hydrophobic material.Described padded 4 is used for the limitation to conversion zone, in order to try
Agent point sample, when required amount of reagent is larger, liquid is unlikely to overflow, and on the conversion zone 31 being limited on bottom plate 3.Separately
Outside, when sample flows into conversion zone 31, padded 4 can to participate in the sample size increase reacted.
Embodiment three
Fig. 4 is the structural representation of the third embodiment of the utility model, is similarly and detects strip for liquid sample
Structure.Difference with embodiment two is, the gas outlet 12 on cover plate 1 be not located at the end of extensional pipeline 24 just on
Side, but positioned at the surface at the midpoint of extensional pipeline 24, now, the ventilation mouth that gas outlet 12 limits with channel plate 2 is (in figure not
Mark) it is located at the midpoint of extensional pipeline 24 so that and the ventilation mouth that extensional pipeline 24 is limited by gas outlet 12 is in communication with the outside.
During the use of this embodiment, due to the midpoint of extensional pipeline 24 ventilation mouth by the gas outlet 12 on cover plate 1 with it is outer
Boundary connects.
Setting by this way, it may be such that blood sample to be measured is evenly more reliably full of from sample cavity 21 through fluid course
Conversion zone 31, preferably to improve the repeatability of test result and reliability, while it can avoid filling conversion zone in liquid
Bubble is produced in conversion zone 31 during 31, preferably to exempt to influence reaction effect.
Example IV
It is lower section not hollow out such as embodiment 1, but as the groove of fluid flow paths 22.Fig. 5 is shown containing non-
The left view of the channel plate 2 of hollow out groove.By the setting of non-hollow out groove, when liquid flows in fluid flow paths 22,
It can smoothly be flowed into using channel bottom to rely in conversion zone 31.
Embodiment five
Circulate experiment test
In order to verify the advantage of the utility model structure, the liquid flow by following experiment test after blood sample point sample is emotionally
There is probability of occurrence of liquid bubble or underfill etc. in condition, and device.
Method of testing：
Strip A and detection strip B (the concrete structure such as institutes of embodiment two of the present utility model will be detected existing for prior art
Set) Experimental comparison is carried out, wherein, structure differences of the detection strip A with detecting strip B essentially consists in, and detection strip A's is ventilative
Mouthful be arranged on opening, and the ventilation mouth for detecting strip B be arranged on extensional pipeline wherein on, both remaining structures
It is same to set, to form the effect of quantitative experiment.
In experimentation, to detection strip A and detect strip B sample cavity respectively and click and enter 10 μ l, blood sample, blood sample point
The fluid flow paths through strip are not flowed into conversion zone through opening.
Test result：
Fig. 6 and Fig. 7 shows the difference of this experiment detection strip A and detection strip B water conservancy diversion effect, in an experiment, such as
Shown in Fig. 6, the blood samples in strip A are detected during reaction chamber is filled, it can be clearly seen that vortex or turbulent flow, also
The discontented phenomenon of filling occurs.In contrast to this, as shown in fig. 7, blood sample in detection strip B can very uniformly, it is steady,
Reliably it is full of reaction chamber.
In addition, showing through experiment is repeated several times, detection strip B can ensure that blood sample 100% is equal during water conservancy diversion
It is even to be smoothly full of reaction chamber, and detect strip A and bubble or the phenomenon not being full of then occurs, its incidence up to 5% or with
On.
Also, when being detected using strip of the present utility model, blood sample is from point sample to full of needed for reaction zone
Time it is shorter, 4-5s can be full of reaction zone, can further improve signal quality.
Provable by above-mentioned circulation experiment test, liquid sample provided by the utility model detects strip, may be such that
Blood sample to be measured is evenly more reliably full of conversion zone from sample cavity through fluid course, with improve test result repeatability and
Reliability, while can avoid producing bubble in conversion zone during liquid fills conversion zone, reaching does not influence to react
The technical purpose of effect.
Above-mentioned embodiment is only preferred embodiment of the present utility model, it is impossible to is protected with this to limit the utility model
Scope, the change of any unsubstantiality that those skilled in the art is done on the basis of the utility model and replacing belongs to
In the utility model scope claimed.
Claims (26)
1. a kind of liquid sample guiding device, including cover plate and the overlappable channel plate below cover plate, one end on the cover plate
Provided with sample holes, the other end is provided with gas outlet；The channel plate is provided with sample cavity, fluid flow paths and opening, wherein, when
When the cover plate closed assembly is on the channel plate, the sample cavity is located at the lower section of the sample holes, and passes through the liquid
Circulation passage connects the opening, it is characterised in that the opening of the channel plate is also associated with extensional pipeline, and it prolongs
Stretch direction be relative to the sample cavity and the liquid communication pipeline other side, when the cover plate closed assembly in channel plate it
When upper, the channel plate limits a ventilation mouth communicated with the external world by gas outlet, and the ventilation mouth is located at the extension
On any point of pipeline, or the ventilation mouth at least partly contacts with the extensional pipeline.
2. guiding device as claimed in claim 1, it is characterised in that the aperture of the gas outlet is 0.1mm-5mm.
3. guiding device as claimed in claim 1, it is characterised in that the cross-sectional area of the extensional pipeline and the liquid communication
The ratio between cross-sectional area of passage is 1:1–1:10.
4. guiding device as claimed in claim 1, it is characterised in that the length of the extensional pipeline and the fluid flow paths
Length ratio be 1:1–1:10.
5. guiding device as claimed in claim 1, it is characterised in that the tube wall of the fluid flow paths is at least partially hydrophilic
Material, and the both ends of fluid connection passage are run through at the water wetted material position.
6. guiding device as claimed in claim 1, it is characterised in that at least partially hydrophobic material of the tube wall of the extensional pipeline
Material.
7. guiding device as claimed in claim 6, it is characterised in that the extensional pipeline is hydrophobic material close to air outlet parts
Material, and be water wetted material close to circulation bore portion.
8. guiding device as claimed in claim 7, it is characterised in that the length of the water wetted material part is 1-2mm.
9. guiding device as claimed in claim 1, it is characterised in that the sample cavity, the fluid flow paths, the circulation
Hole, the extensional pipeline and the ventilation mouth are respectively positioned on same straight line.
10. guiding device as claimed in claim 1, it is characterised in that the sample cavity is located at the underface of the sample holes.
11. the guiding device as described in claim any one of 1-10, it is characterised in that the area of the ventilation mouth with it is described enter
The area ratio in sample hole is 1:1–1:100.
12. a kind of liquid sample detects strip, it is characterised in that contains guiding device, institute as described in claim 1-10 is any
State strip and also contain substrate, the substrate is provided with conversion zone, described when the substrate closed assembly is under the channel plate
Opening is located on the conversion zone and least partially overlapped with it.
13. as claimed in claim 12 detection strip, it is characterised in that the ventilation mouth away from the conversion zone end away from
From for 1mm -10mm.
14. detection strip as claimed in claim 12, it is characterised in that the fluid flow paths are groove.
15. detection strip as claimed in claim 14, it is characterised in that the groove is non-hollow out or hollow out, when described
Groove for hollow out when, and when the upper and lower surface of channel plate distinguishes closed assembly cover plate and substrate, limited jointly by them
The space come forms circulation duct.
16. detection strip as claimed in claim 15, it is characterised in that at least partially hydrophilic material of the tube wall of the circulation duct
Material, and the both ends of fluid connection passage are run through at the water wetted material position.
17. detection strip as claimed in claim 12, it is characterised in that the conversion zone includes water wetted material.
18. detection strip as claimed in claim 12, it is characterised in that the cover plate closed assembly is described on the channel plate
Substrate closed assembly is under the channel plate.
19. detection strip as claimed in claim 12, it is characterised in that electrode is provided with the conversion zone.
20. detection strip as claimed in claim 19, it is characterised in that the conversion zone is also loaded with reaction reagent in advance.
21. as claimed in claim 20 detection strip, it is characterised in that the reaction reagent be dry reagent or by pretreatment into
Dry reagent.
22. detection strip as claimed in claim 14 a, it is characterised in that use is additionally provided between the channel plate and the substrate
In limiting padded of the conversion zone wall body increased above the conversion zone.
23. detection strip as claimed in claim 12, it is characterised in that the detection strip is blood testing strip.
24. detection strip as claimed in claim 23, it is characterised in that the blood testing strip detects for prothrombin time
Strip.
25. a kind of detecting instrument containing detection strip as claimed in claim 12, it is characterised in that also adopted containing reaction signal
Collect equipment or signal processing and analysis equipment, for the reacted physical/chemical of the conversion zone of the detection strip to be believed
Number conduction is to signal collecting device, or conduction to the signal processing and analysis equipment and is further analyzed analysis and result shows
Show.
26. detecting instrument as claimed in claim 25, it is characterised in that the detecting instrument is prothrombin time detector.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621113883.8U CN206756858U (en) | 2016-10-11 | 2016-10-11 | A kind of liquid sample guiding device, detection strip and detecting instrument |
| PCT/CN2016/109480 WO2018068377A1 (en) | 2016-10-11 | 2016-12-12 | Liquid sample guiding device and test apparatus comprising same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621113883.8U CN206756858U (en) | 2016-10-11 | 2016-10-11 | A kind of liquid sample guiding device, detection strip and detecting instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206756858U true CN206756858U (en) | 2017-12-15 |
Family
ID=60607446
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621113883.8U Active CN206756858U (en) | 2016-10-11 | 2016-10-11 | A kind of liquid sample guiding device, detection strip and detecting instrument |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN206756858U (en) |
| WO (1) | WO2018068377A1 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107918031A (en) * | 2016-10-11 | 2018-04-17 | 赵天贤 | A kind of liquid sample guiding device and the detection device containing the guiding device |
| CN109682960A (en) * | 2019-02-26 | 2019-04-26 | 苏州首通科技发展有限公司 | A kind of blomelicalbloodgasandelectrolrteanalyzers testing piece |
| WO2019237742A1 (en) * | 2018-06-12 | 2019-12-19 | 南京岚煜生物科技有限公司 | Microfluidic detection chip for multi-channel quick detecting |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9413525D0 (en) * | 1994-07-05 | 1994-08-24 | Unilever Plc | Improvements in or relating to electrochemical instruments |
| CN100451639C (en) * | 2002-10-25 | 2009-01-14 | 爱科来株式会社 | Analytical tool |
| CN2751307Y (en) * | 2004-12-09 | 2006-01-11 | 泰博科技股份有限公司 | Electrochemical biological detect test paper |
| CN2788184Y (en) * | 2005-04-22 | 2006-06-14 | 北京怡成生物电子技术有限公司 | Biological testing bar |
| KR100890988B1 (en) * | 2007-10-29 | 2009-03-31 | 주식회사 아이센스 | Electrochemical biosensor equipped with sampling port which enables uniform introduction of a small amount of sample |
| US20110079522A1 (en) * | 2009-10-02 | 2011-04-07 | Lifescan Scotland Limited | Multi-analyte test strip with inline working electrodes and shared opposing counter/reference electrode |
-
2016
- 2016-10-11 CN CN201621113883.8U patent/CN206756858U/en active Active
- 2016-12-12 WO PCT/CN2016/109480 patent/WO2018068377A1/en active Application Filing
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107918031A (en) * | 2016-10-11 | 2018-04-17 | 赵天贤 | A kind of liquid sample guiding device and the detection device containing the guiding device |
| WO2019237742A1 (en) * | 2018-06-12 | 2019-12-19 | 南京岚煜生物科技有限公司 | Microfluidic detection chip for multi-channel quick detecting |
| EP3698872A4 (en) * | 2018-06-12 | 2020-09-02 | Nanjing Lansion Biotechnology Co., Ltd. | Microfluidic detection chip for multi-channel quick detecting |
| US11440006B2 (en) | 2018-06-12 | 2022-09-13 | Lansion Biotechnology Co., Ltd | Microfluidic detection chip for multi-channel rapid detection |
| CN109682960A (en) * | 2019-02-26 | 2019-04-26 | 苏州首通科技发展有限公司 | A kind of blomelicalbloodgasandelectrolrteanalyzers testing piece |
| CN109682960B (en) * | 2019-02-26 | 2023-09-29 | 苏州首通科技发展有限公司 | Test piece for electrolyte analyzer |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2018068377A1 (en) | 2018-04-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Weng et al. | Recent advances in thread-based microfluidics for diagnostic applications | |
| CN103182334B (en) | Preparation method and application of electrochemical micro-fluidic sensing chip | |
| WO2020177773A1 (en) | Multi-channel microfluidic blood coagulation measurement chip having five-layer structure | |
| CN102803943B (en) | Breathe condensed fluid sampler and detecting device and breathing/breathing condensed fluid sampler and detecting device | |
| CN104042252B (en) | Rotatable fluid sample collection device | |
| CN107918031A (en) | A kind of liquid sample guiding device and the detection device containing the guiding device | |
| CN206756858U (en) | A kind of liquid sample guiding device, detection strip and detecting instrument | |
| JP4660662B2 (en) | cartridge | |
| CN217410812U (en) | Multi-channel thromboelastogram detection chip based on micro-fluidic | |
| JP2014515494A (en) | Capillary liquid flow measurement and capillary flow apparatus therefor | |
| CN105921185A (en) | Urine multi-index detection microfluidic device and preparation method thereof | |
| CN110146568A (en) | A kind of multi-parameter electrochemical detection electrode piece | |
| CN105802847A (en) | Cell analyzer | |
| WO2020244517A1 (en) | Microfluidic chip and manufacturing method therefor | |
| CN108169129A (en) | A kind of method for realizing microballoon single-row sample introduction | |
| CN208824517U (en) | Bed is other to diagnose micro-fluidic chip | |
| CN211577039U (en) | Micro-fluidic impedance type biological online detection device | |
| CN110249221A (en) | For detecting the vertical current dynamic formula test device of the concentration of glucose in fluid sample | |
| CN115327138A (en) | Portable fecal occult blood detection device | |
| CN204789248U (en) | With compatible microparticle detection device of process | |
| CN209640280U (en) | A kind of multi-channel fluid hemostasis examination chip with five-layer structure | |
| CN111024768A (en) | Micro-fluidic impedance type biological online detection device | |
| CN207472819U (en) | A kind of portable blood test kit device based on electrochemical method | |
| TW201500096A (en) | A blood plasma separating device | |
| SE503669C2 (en) | Procedure for analysis and apparatus for carrying out the procedure |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20200529 Address after: 510663 unit 901, zone C2, No. 182, science Avenue, Science City, Guangzhou high tech Industrial Development Zone, Guangzhou, Guangdong Province Patentee after: HELIXGEN (GUANGZHOU) Co.,Ltd. Address before: Unit 901, zone C2, innovation building, No. 182, science Avenue, Guangzhou Co-patentee before: HELIXGEN (GUANGZHOU) Co.,Ltd. Patentee before: Zhao Tianxian |
|
| TR01 | Transfer of patent right |