CN209640280U

CN209640280U - A kind of multi-channel fluid hemostasis examination chip with five-layer structure

Info

Publication number: CN209640280U
Application number: CN201920260135.XU
Authority: CN
Inventors: 许行尚; 杰弗瑞·陈
Original assignee: Nanjing Lanyu Biological Technology Co Ltd
Current assignee: Nanjing Lanyu Biological Technology Co Ltd
Priority date: 2019-03-01
Filing date: 2019-03-01
Publication date: 2019-11-15
Anticipated expiration: 2029-03-01

Abstract

The utility model discloses a kind of multi-channel fluid hemostasis examination chip with five-layer structure, including chip body, the chip body successively includes first layer chip, second layer chip, third layer chip, the 4th layer of chip and layer 5 chip from top to bottom, the first layer chip, second layer chip, third layer chip, the 4th layer of chip match with layer 5 chip and define closed fluid channel and multiple mutually independent sensing chamber, the first layer chip is equipped with well, and the well is connected by the fluid channel with the sensing chamber；The chip body further includes electrode, and indoor correspond of each detection is equipped with the electrode.Detection efficiency is improved by the quantity that five layers of chip design the sensing chamber that can be multiplied so as to disposably detect more coagulation indexes；The multi-channel fluid hemostasis examination chip design with five-layer structure is rationally, simple and compact for structure.

Description

A kind of multi-channel fluid hemostasis examination chip with five-layer structure

Technical field

The utility model belongs to technical field of medical equipment, more particularly, to a kind of multichannel miniflow with five-layer structure Body hemostasis examination chip.

Background technique

Microfluidics be across include engineering science, physics, chemistry, microtechnology and biotechnology diverse discipline apply Technology.Microfluidics be related to research to micro fluid and to how such as micro-fluid chip etc various microfluids The research of such a small amount of fluid is manipulated, controlled and used in system and equipment.Such as: microfluid biochip (referred to as " core Piece laboratory ") it is operated in molecular biology field for integrating chemical examination, to be used for such as enzyme analysis and DNA, detect bioid The purpose of learning toxin and pathogen, diagnosing the illness.

Micro-fluidic chip (microfluidic chip) is current micro-total analysis system (Miniaturized Total Analysis Systems) development hot fields.Microfluidic chip analysis is using chip as operating platform, while with analytical chemistry Based on, it is to rely on micro electro mechanical processing technology, is main application at present with life science using microchannel network as structure feature Object is the emphasis of current micro-total analysis system field development.Its target is the function entire laboratory, including sampling, Dilution, reagent adding, reaction, separation, detection etc. are integrated on microchip.Micro-fluidic chip is the main flat of microflow control technique realization Platform.Its device characteristic is mainly that its resulting structure (channel, sensing chamber and other certain functional components) for accommodating fluid at least exists It is micron order scale on one latitude.Due to micron-sized structure, fluid shows and produces wherein different from macro-scale Property.Therefore the performance that unique analysis generates is developed.The characteristics of micro-fluidic chip and advantages for development: micro-fluidic core Piece has the characteristics that liquid flowing controllable, consumption sample and reagent are few, improve to analysis speed tenfold hundreds of times, it can be with Analysis while carrying out a sample up to a hundred within a few minutes or even shorter time, and can be with the pretreatment of canbe used on line sample And analysis overall process.Its application purpose generated is to realize ultimate aim-chip lab of micro-total analysis system, at present work The focus on the application field for making to develop is life science.

Current international research status: innovation focuses mostly in terms of separation, detection architecture；To how introducing practical sample on chip The problems of product analysis, such as sample is introduced, is varied, the related research of pre-treatment is also very weak.Its development is dependent on more The development of subject crossing.

In Chinese patent literature CN108398470A, a kind of blood activated clotting time measurement bio-sensing is disclosed Device, bottom, middle layer and upper layer including stacking gradually connection；The upper layer is equipped with Loading channel and gas-permeable channels, described to add Sample channel and the equal through-thickness of the gas-permeable channels run through the upper layer；The middle layer is deposited equipped at least one sample Hole, at least one diffusion admittance and at least one detection cell, the detection cell are deposited by the diffusion admittance and the sample Hole connection, the detection cell through-thickness run through the middle layer；The upper surface of the bottom is equipped with working electrode and reference Electrode；The Loading channel is located at the top of the sample deposition hole and is connected to the sample deposition hole；The gas-permeable channels Positioned at the detection cell top and be connected to the detection cell；The bottom and upper layer direction and the corresponding detection cell Surface region and the detection cell pool wall with being encircled at least one sample detection chamber；The working electrode and the reference It is intracavitary that one end of electrode is respectively positioned on the sample detection, and the other end is all extended to be formed except the middle layer and the upper layer and be used In the connecting pin being connect with detecting instrument；The top cavity wall of the sample detection chamber and it is located at the working electrode and the ginseng The dry blood coagulation accelerant coatings than being equipped in the lower chamber wall between electrode.Blood activated clotting time measurement biology passes Though sensor devises multichannel and more sensing chamber while being detected, more sensing chamber use the same reference electrode, and All interference of the design between bottom, such design electrode can be very big for reference electrode and working electrode, in actually detected mistake Cheng Zhong will affect the accuracy of pattern detection result.

Chinese patent literature (application number: 201810599700.5) discloses a kind of multichannel and quickly detects microfluid detection Chip, including chip body are provided with chip thief hatch, multiple mutually independent sensing chamber and miniflow on the chip body Road, the chip thief hatch are connected to by fluid channel with the sensing chamber, and the chip body further includes electrode, the sensing chamber It is connected with the electrode；The fluid channel includes a mainstream channel and multiple points of microfluidic channels, the mainstream channel End distributes multiple point microfluidic channels, multiple described to divide microfluidic channel and multiple mutually independent sensing chamber one by one Corresponding connection；The other end of the mainstream channel is connected to the chip thief hatch.The multichannel quickly detects microfluid detection Chip is three layers of chip, and the size of chip body is about 8~10cm*2.4~2.8cm, thus the size of the chip is bigger than normal.

Therefore, it is necessary to develop a kind of big reasonable detection flux of design, detection efficiency and the equal high and volume of accuracy more The lower multi-channel fluid hemostasis examination chip with five-layer structure of small cost.

Utility model content

The technical problem to be solved by the present invention is to provide a kind of reasonable detection flux of design big, detection efficiency and standard True property height and the lower multi-channel fluid hemostasis examination chip with five-layer structure of the smaller cost of volume.

In order to solve the above technical problems, the technical solution adopted in the utility model is, this has the multichannel of five-layer structure Microfluid hemostasis examination chip, including chip body, the chip body successively include first layer chip, the second layer from top to bottom Chip, third layer chip, the 4th layer of chip and layer 5 chip, the first layer chip, second layer chip, third layer chip, 4th layer of chip is matched with layer 5 chip defines closed fluid channel and multiple mutually independent sensing chamber, and described first Layer chip is equipped with well, and the well is connected by the fluid channel with the sensing chamber；The chip body is also Including electrode, indoor correspond of each detection is equipped with the electrode.

By adopting the above technical scheme, micro-fluidic chip detection has precision high, and speed is fast, the lower feature of testing cost, It is appropriate for the detection that accurate medical rings carry out, defines closed fluid channel and multiple phases by five layers of chip cooperation of design Mutual independent sensing chamber, may be implemented to detect multiple sample index simultaneously in this way；It can be multiplied by five layers of chip design The quantity of sensing chamber improves detection efficiency so as to disposably detect more coagulation indexes；The design is reasonable, structure Simple and compact, the quantity for the sensing chamber that has been multiplied can reduce production cost；Each detection is indoor to be embedded with detection examination in advance Agent applies alternating voltage by electrode and receives the signal that reaction of blood generates in sensing chamber simultaneously；Chip structure is simple, operation side Just, detection efficiency is improved, and greatly reduces the consumption of resource；It realizes quickly detection, reduces costs.

The utility model further improvement lies in that, the second layer chip and the 4th layer of chip run through be equipped with it is described Fluid channel and sensing chamber；The fluid channel includes a mainstream channel and multiple points of microfluidic channels, the end of the mainstream channel End distributes multiple point microfluidic channels, multiple described to divide microfluidic channel and multiple mutually independent sensing chamber one a pair of It should be connected to；The front end of the mainstream channel is connected to the well.By designing the mainstream channel of specific structure form and more It is a to divide microfluidic channel, for the flowing of guide blood sample, so that by a mainstream channel and multiple points of microfluidic channels, Can sample and non-contaminated samples, easy sample introduction be injected to multiple sensing chamber simultaneously；After well sample introduction, by the mainstream channel same time-division Multiple points of microfluidic channels are not flowed to, enter back into multiple mutually independent sensing chamber, and each sensing chamber is by dividing microfluidic channel It is extended to form at its end to both ends and to extending out, i.e., the width of sensing chamber is greater than the coupled width for dividing microbody channel Degree, such setting makes detection sample be easier faster flow detection room, and detects interior and be embedded with detection reagent in advance, It may be implemented to detect multiple indexs simultaneously in this way, have the effect of multichannel；Chip structure is simple, easy to operate, improves inspection Efficiency is surveyed, and greatly reduces the consumption of resource；It realizes quickly detection, reduces costs.

The utility model further improvement lies in that, be equipped with multiple rows on the first layer chip and the layer 5 chip Stomata, multiple gas vents be arranged at one end of the first layer chip and the layer 5 chip and setting with it is described It is corresponded at the corresponding position of sensing chamber and with multiple sensing chamber.By running through in upper layer chip, multiple exhausts are set Hole, so that the flow resistance of fluid to be measured reduces, flowing more rapidly, realizes Fast Filling sensing chamber；The setting of gas vent is advantageous In the flowing of sample, facilitate sample introduction, if being not provided with gas vent, sample cannot flow into sensing chamber and be reacted, and detection is indoor Embedding detection reagent in advance.In addition, setting of the gas vent in layer 5, can't cause the leakage of whole blood sample, because of blood More sticky, flowing needs the cooperation of the hydrophilic interface on two sides, and the position of gas vent is (i.e. separate to divide miniflow in the end of sensing chamber The one end in body channel) tangent position, blood flow to herein when, the hollow out setting of gas vent makes it only contact unilateralis hydrosphere Face, so blood will not continue to flow forward and leak.

The utility model further improvement lies in that, the electrode is arranged on the third layer chip or setting is described the On three layers of chip and the layer 5 chip；The electrode includes reference electrode and working electrode, and each detection is indoor It corresponds and is equipped with the reference electrode and the working electrode.In each detection interior, reference electrode and work electricity are set simultaneously The consistency of the testing result of each sensing chamber can extremely be effectively ensured.

As the optimal technical scheme of the utility model, the working electrode and the reference electrode are arranged at described the On three layers of chip, or it is arranged on the third layer chip and the layer 5 chip；The working electrode and the reference One end of electrode is respectively positioned on that the detection is indoor, the other end all extend to the third layer chip end or the third layer core The end of piece and the layer 5 chip forms test side；Divide the corresponding multiple detections of microfluidic channel indoor with described It is independent to be equipped with a working electrode and independently lead to the test side, divide microfluidic channel corresponding multiple institutes with described Stating the indoor reference electrode of detection is that the test side is led to after being serially connected.By the way that each detection is indoor Reference electrode is serially connected, and further makes each reference electrode and each working electrode in the same position of each sensing chamber Resistance measurement is carried out, the consistency for guaranteeing the testing result of each sensing chamber is more advantageous to；Reference electrode and working electrode are being examined End is surveyed to be in rectangle and flush setting with the end of third layer chip or/and layer 5 chip.

The utility model further improvement lies in that, the reference electrode include the first reference electrode and the second reference electrode, First reference electrode and second reference electrode are separately positioned, and first reference electrode is arranged in the third layer core The back side of the third layer chip or the front of the layer 5 chip is arranged in the front of piece, second reference electrode.

The utility model further improvement lies in that, the working electrode include the first working electrode and the second working electrode, First working electrode and second working electrode are separately positioned, and first working electrode is arranged in the third layer core The back side of the third layer chip or the front of the layer 5 chip is arranged in the front of piece, second working electrode.

As the optimal technical scheme of the utility model, one end of first working electrode and first reference electrode It is indoor that the detection is respectively positioned in the front of the third layer chip；First working electrode and first reference electrode The other end all extends to the end of one end of the first layer chip and the second layer chip in the front of the third layer chip Except head and form the test side one being connected with detecting instrument.First working electrode and the first reference electrode and second layer chip On sensing chamber be arranged in a one-to-one correspondence, guarantee second layer chip on sensing chamber electrochemical signals acquisition and detection.

As the optimal technical scheme of the utility model, one end of second reference electrode and second working electrode It is indoor to be respectively positioned on the detection；Second reference electrode and the second working electrode other end are in the third layer chip The back side all extends to except the end of one end of the 4th layer of chip and the layer 5 chip and is formed and the detector The other end of test side two or second reference electrode and second working electrode that device is connected is in the layer 5 core The front of piece all extends to except the end of one end of the third layer chip and the 4th layer of chip and is formed and the inspection Survey the test side three that instrument is connected.Sensing chamber one on second working electrode and the second reference electrode and the 4th layer of chip is a pair of It should be arranged, guarantee the acquisition and detection of the electrochemical signals of the sensing chamber on the 4th layer of chip.

As the optimal technical scheme of the utility model, the exposed end in the test side one in the test side three it Outside.Such setting can be to avoid on the first working electrode and the first reference electrode and layer 5 chip on third layer chip Interference between second working electrode and the second reference electrode, and make the multi-channel fluid hemostasis examination with five-layer structure Test side one after chip insertion detecting instrument can directly be connected with detecting instrument simultaneously with test side three.

The utility model further improvement lies in that, it is corresponding with the mainstream channel multiple described to divide phase in microfluidic channel The length of point microfluidic channel described in adjacent two is all different, so that adjacent, multiple described to divide microfluidic channel to connect more A sensing chamber forms the setting that is dislocatedly distributed.Such setting can reduce the size of the chip body, reduce cost.

As the optimal technical scheme of the utility model, the second layer chip is equipped with liquid receiving port one, the third layer core Piece is equipped with liquid receiving port two, and the 4th layer of chip is equipped with liquid receiving port three, the liquid receiving port one, the liquid receiving port two and described connects liquid Mouth three is corresponding with the position of the well to be arranged and is connected with the well；The mainstream channel includes first Mainstream channel and the second mainstream channel, it is described that microfluidic channel is divided to include that first point of microfluidic channel and second point of microfluid are logical Road, first mainstream channel and first point of microfluidic channel, which run through, to be arranged on the second layer chip, and described second Mainstream channel and second point of microfluidic channel, which run through, to be arranged on the 4th layer of chip, and the one of first mainstream channel End is connected with the liquid receiving port one, and the other end is connected one to one multiple described by multiple first point of microfluidic channel Sensing chamber；One end of second mainstream channel is connected with the liquid receiving port three, and the other end is micro- by multiple described second points Fluid channel connects one to one multiple sensing chamber.After test sample enters chip by well, pass through liquid receiving port one The first mainstream channel and liquid receiving port two are flowed to respectively, liquid receiving port three is flowed to by liquid receiving port two, flow to second by liquid receiving port three Mainstream channel flows to each sensing chamber of second layer chip and each sensing chamber of the 4th layer of chip to realize simultaneously respectively.

As the optimal technical scheme of the utility model, first mainstream channel to far from the liquid receiving port one one end Horizontally extending to be equipped with first point of microfluidic channel, one connecting detection room one, first mainstream channel connects to far from described One end of liquid mouth one vertically extends to two sides respectively is equipped with first point of microfluidic channel two and first point of microfluidic channel Three, first point of microfluidic channel two extends to both ends respectively along the direction parallel with first mainstream channel is connected with inspection Room two and sensing chamber three are surveyed, first point of microbody channel three is along the direction parallel with first mainstream channel respectively to both ends Extension is connected with sensing chamber four and sensing chamber five.It is logical by the mainstream channel and multiple points of microfluids that design specific structure form Road, for the flowing of guide blood sample, so that a sample chamber, can inject sample to multiple reaction chambers simultaneously, make Faster, raising detection efficiency must be flowed；It is solidifying to reduce the multi-channel fluid with five-layer structure for such design simultaneously The multi-channel fluid blood coagulation for the three-decker applied before the size of blood detection chip, long and wide length and the applicant is examined Chip is surveyed compared to reducing 25~45%, reduces production cost, and keep chip more small and exquisite portable.

As the optimal technical scheme of the utility model, second mainstream channel to far from the liquid receiving port three one end Horizontally extending to be equipped with second point of microfluidic channel, one connecting detection room six, second mainstream channel connects to far from described One end of liquid mouth three vertically extends to two sides respectively is equipped with second point of microfluidic channel two and second point of microfluidic channel Three, second point of microfluidic channel two extends to both ends respectively along the direction parallel with second mainstream channel is connected with inspection Room seven and sensing chamber eight are surveyed, second point of microbody channel three is along the direction parallel with second mainstream channel respectively to both ends Extension is connected with sensing chamber nine and sensing chamber ten.It is logical by the mainstream channel and multiple points of microfluids that design specific structure form Road, for the flowing of guide blood sample, so that a sample chamber, can inject sample to multiple reaction chambers simultaneously, make Faster, raising detection efficiency must be flowed；It is solidifying to reduce the multi-channel fluid with five-layer structure for such design simultaneously The size of blood detection chip, long and wide length is compared with the multi-channel fluid hemostasis examination chip applied before the applicant 25~45% are reduced, production cost is reduced, and keeps chip more small and exquisite portable.

As the optimal technical scheme of the utility model, the liquid receiving port one, the liquid receiving port two and the liquid receiving port three Size be all larger than or equal to the well size；Multiple gas vents are arranged at the end with multiple sensing chamber Hold the top of tangent position；The quantity of the first layer chip and the gas vent on the layer 5 chip is 5. The end of sensing chamber is far from the one end for dividing microfluidic channel, each sensing chamber is provided with a gas vent.

As the optimal technical scheme of the utility model, each test side, test side one, test side two and test side Three shape is that the spacing between rectangle and each test side is equal.

As the optimal technical scheme of the utility model, the first layer chip, second layer chip, third layer chip, Four layers of chip are bonded by way of glued integral with layer 5 chip.Preferably, first layer chip, second layer chip, Third layer chip, the 4th layer of chip and layer 5 chip are bonded into one by double gluings.

As the optimal technical scheme of the utility model, the second layer chip and the 4th layer of chip are pressure sensitive adhesive tape, institute The material for stating first layer chip, third layer chip and layer 5 chip is any one of PMMA, PP, PE, PET, and described The surface of first layer chip and the layer 5 chip all has hydrophilic film, flows into sample quickly through the well Mainstream channel, then it is diverted to each point of microfluidic channel.It adopts this technical solution, material is easy to get, and the production of pressure sensitive adhesive tape Technique, so adopting this technical solution, can be accurately controlled the depth and size of fluid channel with its thickness of the control of precision, It is also convenient for the depth of control sensing chamber simultaneously, so that the thickness deviation of each sensing chamber of micro-fluid chip is small, consistency is high, mentions The high accuracy of detection；The surface of first layer chip and layer 5 chip is respectively provided with hydrophilic film, can make sample more quickly Mainstream channel is flowed by well and is diverted to each point of microfluidic channel, in this way quickening flowing velocity, and detection can be improved Efficiency.

As the optimal technical scheme of the utility model, the thickness of the second layer chip and the 4th layer of chip is 0.1 ~1.0mm；The first layer chip, the front of second layer chip and third layer chip and the 4th layer of chip, layer 5 chip with It is 0.1~1.0mm that the back side of third layer chip, which matches and defines the depth of closed fluid channel, matches the institute defined The width for stating sensing chamber is 1.0~2.0mm.

Divide microfluidic channel in the company with the sensing chamber as the optimal technical scheme of the utility model, described in each The place of connecing all has nozzle；The electrode with a thickness of 0.5mm.It is equipped with nozzle in the junction of point microfluidic channel and sensing chamber In order to make sample more easily flow into sensing chamber more quickly；The effect of electrode is to apply alternating voltage while receiving blood in sensing chamber The signal that liquid reaction generates.

Compared with prior art, closed fluid channel and multiple mutually indepedent should be defined by design five layers of chip cooperation Sensing chamber, the multiple indexs for detecting sample simultaneously may be implemented in this way；The detection that can be multiplied is designed by five layers of chip The quantity of room, so as to disposably detect more coagulation indexes；Five layers of chip are designed, 10 sensing chamber are shared, are cooperated Corresponding electrode, single injected sampling can detecte 10 coagulation indexes；The design is reasonable, simple and compact for structure, and be multiplied inspection The quantity for surveying room can reduce production cost；Each detection is indoor to be embedded with detection reagent in advance, applies alternating current by electrode Pressure while the signal for receiving reaction of blood generation in sensing chamber；Chip structure is simple, easy to operate, improves detection efficiency, and Greatly reduce the consumption of resource；It realizes quickly detection, reduces costs；Pass through the major flow of design specific structure form simultaneously Road and multiple points of microfluidic channels, for the flowing of guide blood sample, so that a sample chamber, it can be simultaneously to multiple anti- Chamber injection sample and non-contaminated samples, easy sample introduction are answered, the size of the multi-channel fluid chip, long and wide length are reduced 25~45% are reduced (before compared with the multi-channel fluid hemostasis examination chip for the three-decker applied before the applicant Length and width be 8~10cm*2.4~2.8cm, the length and width of the multi-channel fluid hemostasis examination chip be 5~7cm*1.6~ 2.0cm), production cost is reduced, and keeps chip more small and exquisite portable.

Detailed description of the invention

It is further described with reference to the accompanying drawing with the embodiments of the present invention:

Fig. 1 is the multi-channel fluid hemostasis examination chip perspective knot with five-layer structure of the utility model embodiment 1 Structure schematic diagram；

Fig. 2 is the chip of the multi-channel fluid hemostasis examination chip with five-layer structure of the utility model embodiment 1 Decomposition texture schematic diagram；

Fig. 3 is the back side of the multi-channel fluid hemostasis examination chip with five-layer structure of the utility model embodiment 1 Structural schematic diagram；

Fig. 4 is the third of the multi-channel fluid hemostasis examination chip with five-layer structure of the utility model embodiment 1 The decomposition texture schematic diagram of the front and back of layer chip；

Fig. 5 is the 5 of the multi-channel fluid hemostasis examination chip with five-layer structure of the utility model embodiment 1 The decomposition texture schematic diagram of the front and back of layer chip；

Fig. 6 is the multi-channel fluid hemostasis examination chip perspective knot with five-layer structure of the utility model embodiment 2 Structure schematic diagram；

Fig. 7 is the chip of the multi-channel fluid hemostasis examination chip with five-layer structure of the utility model embodiment 2 Decomposition texture schematic diagram；

Fig. 8 is the back side of the multi-channel fluid hemostasis examination chip with five-layer structure of the utility model embodiment 2 Structural schematic diagram；

Fig. 9 is the third of the multi-channel fluid hemostasis examination chip with five-layer structure of the utility model embodiment 2 The decomposition texture schematic diagram of the front and back of layer chip；

Figure 10 is the 5 of the multi-channel fluid hemostasis examination chip with five-layer structure of the utility model embodiment 2 The decomposition texture schematic diagram of the front and back of layer chip；

Wherein: 1- first layer chip；2- second layer chip；201- liquid receiving port one；3- third layer chip；301- liquid receiving port Two；The 4th layer of chip of 4-；401- liquid receiving port three；5- layer 5 chip；6- fluid channel；601- mainstream channel；The first mainstream of 6011- Channel；The second mainstream channel of 6012-；602- divides microfluidic channel；6021- first divides microfluidic channel；6022- second divides miniflow Body channel；7- gas vent；8- well；9- sensing chamber；10- reference electrode；The first reference electrode of 1001-；The second reference of 1002- Electrode；11- working electrode；The first working electrode of 1101-；The second working electrode of 1102-；The test side 12-；The test side 1201- one； The test side 1202- two；The test side 1203- three.

Specific embodiment

Embodiment 1: as shown in Fig. 1~5, this has the multi-channel fluid hemostasis examination chip of five-layer structure, including core Piece ontology, the chip body successively include first layer chip 1, second layer chip 2, third layer chip 3, the 4th layer from top to bottom Chip 4 and layer 5 chip 5, the first layer chip 1, second layer chip 2, third layer chip 3, the 4th layer of chip 4 and the 5th Layer chip 5, which matches, defines closed fluid channel and multiple mutually independent sensing chamber 9, and the first layer chip 1 is equipped with Well 8, the well 8 are connected by the fluid channel 6 with the sensing chamber 9；The chip body further includes electrode, It is corresponded in each sensing chamber 9 and is equipped with the electrode；The second layer chip 2 and the 4th layer of chip 4 pass through It is equipped with the fluid channel 6 and sensing chamber 9；The fluid channel 6 includes a mainstream channel 601 and multiple points of microfluidic channels 602, the end of the mainstream channel 601 distributes multiple point microfluidic channels 602, multiple described to divide microfluidic channel 602 are connected to multiple mutually independent one-to-one correspondence of sensing chamber 9；The front end of the mainstream channel 601 and the well 8 connect It is logical；Each sensing chamber 9 is extended to form to both ends and to extending out at its end from point microfluidic channel 602, i.e. the width of sensing chamber 9 Degree is greater than the coupled width for dividing microbody channel 602, and such setting flows to detection sample more easily more quickly Sensing chamber 9, and it is embedded with detection reagent in advance in sensing chamber 9；It is all provided on the first layer chip 1 and the layer 5 chip 5 Have multiple gas vents 7, multiple gas vents 7 be arranged at one end of the first layer chip 1 and the layer 5 chip 5 and It is arranged at position corresponding with the sensing chamber 9 and is corresponded with multiple sensing chamber 9；The electrode is arranged in institute It states on third layer chip 3；The electrode includes reference electrode 10 and working electrode 11, uniform a pair in each sensing chamber 9 The reference electrode 10 and the working electrode 11 should be equipped with；The working electrode 11 is arranged at institute with the reference electrode 10 It states on third layer chip 3；One end of the working electrode 11 and the reference electrode 10 is respectively positioned in the sensing chamber 9, another The end that end all extends to the third layer chip 3 forms test side 12；Divide microfluidic channel 602 corresponding multiple institutes with described It states and is independently equipped with a working electrode 11 in sensing chamber 9 and independently leads to the test side 12, divide miniflow with described The reference electrode 10 in the corresponding multiple sensing chamber 9 in body channel 602 is that the inspection is led to after being serially connected Survey end 12；Reference electrode 10 in rectangle and flushes setting with the end of third layer chip 3 in test side 12 with working electrode 11； The reference electrode 10 includes the first reference electrode 1001 and the second reference electrode 1002, first reference electrode 1001 and institute State that the second reference electrode 1002 is separately positioned, the front of the third layer chip 3, institute is arranged in first reference electrode 1001 State the back side that the third layer chip 3 is arranged in the second reference electrode 1002；The working electrode 11 includes the first working electrode 1101 and second working electrode 1102, first working electrode 1101 and second working electrode 1102 are separately positioned, institute The front that the third layer chip 3 is arranged in the first working electrode 1101 is stated, second working electrode 1102 is arranged described The back side of third layer chip 3；One end of first working electrode 1101 and first reference electrode 1001 is in the third The front of layer chip 3 is respectively positioned in the sensing chamber 9；First working electrode 1101 and first reference electrode 1001 The other end all extends to one end of the first layer chip 1 and the second layer chip 2 in the front of the third layer chip 3 Except end and form the test side 1 being connected with detecting instrument；Second reference electrode 1002 and second work The one end for making electrode 1102 is respectively positioned in the sensing chamber 9；Second reference electrode 1002 and second working electrode 1102 other ends all extend to the one of the 4th layer of chip 4 and the layer 5 chip 5 at the back side of the third layer chip 3 Except the end at end and form the test side 2 1202 being connected with the detecting instrument；The i.e. described first layer chip 1 with it is described The end of second layer chip 2 flushes and flushes with the end of the 4th layer of chip 4 and layer 5 chip 5, the third layer core The test side 1 and test side 2 1202 formed on piece 3 it is exposed the end of first layer chip 1 and second layer chip 2 with The end of 4th layer of chip 4 and layer 5 chip 5；It is corresponding with the mainstream channel 601 multiple described to divide microfluidic channel 602 In the length of point microfluidic channel 602 described in adjacent two be all different multiple described divide microfluidic channel so that adjacent Multiple sensing chamber 9 of 602 connections form the setting that is dislocatedly distributed；Such setting can reduce this with the more of five-layer structure The size of channel microfluid hemostasis examination chip, reduces cost；The second layer chip 2 is equipped with liquid receiving port 1, the third Layer chip 3 is equipped with liquid receiving port 2 301, and the 4th layer of chip 4 is equipped with liquid receiving port 3 401, and the liquid receiving port 1 described connects The setting corresponding with the position of the well 8 of liquid mouth 2 301 and the liquid receiving port 3 401 and with 8 phase of well Connection；The mainstream channel 601 includes the first mainstream channel 6011 and the second mainstream channel 6012, described to divide microfluidic channel 602 include first point of microfluidic channel 6021 and second point of microfluidic channel 6022, first mainstream channel 6011 and described First point of microfluidic channel 6021 is through being arranged on the second layer chip 2, second mainstream channel 6012 and described the Two points of microfluidic channels 6022, which run through, to be arranged on the 4th layer of chip 4, one end of first mainstream channel 6011 and institute It states liquid receiving port 1 to be connected, the other end is connected one to one multiple institutes by multiple first point of microfluidic channel 6021 State sensing chamber 9；One end of second mainstream channel 6012 is connected with the liquid receiving port 3 401, and the other end passes through multiple institutes Second point of microfluidic channel 6022 is stated to connect one to one multiple sensing chamber 9；Test blood sample is entered by well 8 After chip, the first mainstream channel 6011 and liquid receiving port 2 301 are flowed to by liquid receiving port 1 respectively, then pass through liquid receiving port 2 301 Liquid receiving port 3 401 is flowed to, the second mainstream channel 6012 is flowed to by liquid receiving port 3 401, flows to second simultaneously respectively to realize Layer each sensing chamber of chip 2 and each sensing chamber 9 of the 4th layer of chip 4；First mainstream channel 6011 is to far from described One end of liquid receiving port 1 is horizontally extending to be equipped with first point of microfluidic channel, one connecting detection room one, and described first is main Circulation road 6011 vertically extends to two sides respectively to one end far from the liquid receiving port 1 is equipped with first point of microfluid Channel two and first point of microfluidic channel three, first point of microfluidic channel two is along parallel with first mainstream channel 6011 Direction extend to both ends be connected with sensing chamber two and sensing chamber three respectively, first point of microbody channel three is along with described first The parallel direction of mainstream channel 6011 extends to both ends respectively is connected with sensing chamber four and sensing chamber five；Second mainstream channel 6012 are equipped with second point of microfluidic channel, one connecting detection room to one end far from the liquid receiving port 3 401 is horizontally extending Six, second mainstream channel 6012 vertically extends to two sides respectively to one end far from the liquid receiving port 3 401 to be set There are second point of microfluidic channel two and second point of microfluidic channel three, second point of microfluidic channel two is led along with described second The parallel direction of circulation road 6012 extends to both ends respectively is connected with sensing chamber seven and sensing chamber eight, second point of microbody channel Three are connected with sensing chamber nine and sensing chamber ten to both ends extension respectively along the direction parallel with second mainstream channel 6012；I.e. The quantity of the sensing chamber 9 of second layer chip 2 is 5, and the quantity of the sensing chamber 9 on the 4th layer of chip 4 is 5, this has five layers The quantity of the sensing chamber 9 of the multi-channel fluid hemostasis examination chip of structure is 10；The liquid receiving port 1 described connects liquid Mouthfuls 2 301 and the liquid receiving port 3 401 size be all larger than or equal to the well 8 size；Multiple gas vents 7 are The top of the position tangent with the end of multiple sensing chamber 9 is set；The first layer chip 1 and the layer 5 chip The quantity of the gas vent 7 on 5 is 5；The end of sensing chamber 9 is separate one end for dividing microfluidic channel 602, each Sensing chamber 9 is provided with a gas vent 7；The shape of each test side 12 is between rectangle and each test side 12 Spacing it is equal.

Embodiment 2: as shown in Fig. 6~10, the difference with embodiment 1 exists, second working electrode 1102 and described The front of the layer 5 chip 5 is arranged in two reference electrodes 1002；Specifically, the multi-channel fluid with five-layer structure Hemostasis examination chip, including chip body, the chip body successively include first layer chip 1, second layer chip from top to bottom 2, third layer chip 3, the 4th layer of chip 4 and layer 5 chip 5, the first layer chip 1, second layer chip 2, third layer chip 3, the 4th layer of chip 4 is matched with layer 5 chip 5 defines closed fluid channel and multiple mutually independent sensing chamber 9, institute First layer chip 1 is stated equipped with well 8, the well 8 is connected by the fluid channel 6 with the sensing chamber 9；It is described Chip body further includes electrode, corresponds in each sensing chamber 9 and is equipped with the electrode；2 He of second layer chip The 4th layer of chip 4 is through equipped with the fluid channel 6 and sensing chamber 9；The fluid channel 6 includes a mainstream channel 601 With multiple points of microfluidic channels 602, the end of the mainstream channel 601 distributes multiple point microfluidic channels 602, multiple Described point of microfluidic channel 602 is corresponded with multiple mutually independent sensing chamber 9 and is connected to；The front end of the mainstream channel 601 It is connected to the well 8；Each sensing chamber 9 is from point microfluidic channel 602 at its end to both ends and to extending out extension shape At that is, the width of sensing chamber 9 is greater than the coupled width for dividing microbody channel 602, and such setting makes to detect sample more It is easy flow detection room 9 more quickly, and is embedded with detection reagent in advance in sensing chamber 9；The first layer chip 1 and described Multiple gas vents 7 are equipped on five layers of chip 5, multiple gas vents 7 are arranged at the first layer chip 1 and the described 5th One end of layer chip 5 and it is arranged at position corresponding with the sensing chamber 9 and is corresponded with multiple sensing chamber 9； The electrode is arranged on the third layer chip 3 and the layer 5 chip 5；The electrode includes reference electrode 10 and work Electrode 11 corresponds in each sensing chamber 9 and is equipped with the reference electrode 10 and the working electrode 11；The work Electrode 11 and the reference electrode 10 are arranged on the third layer chip 3 and the layer 5 chip 5；The working electrode 11 are respectively positioned in the sensing chamber 9 with one end of the reference electrode 10, and the other end all extends to the third layer chip 3 and institute The end for stating layer 5 chip 5 forms test side 12；Divide in the corresponding multiple sensing chamber 9 of microfluidic channel 602 with described It is independent to be equipped with a working electrode 11 and independently lead to the test side 12, divide microfluidic channel 602 right with described The reference electrode 10 in multiple sensing chamber 9 answered is that the test side 12 is led to after being serially connected；Reference Electrode 10 is flushed in rectangle and with the end of third layer chip 3 or/and layer 5 chip 5 with working electrode 11 in test side 12 Setting；The reference electrode 10 includes the first reference electrode 1001 and the second reference electrode 1002, first reference electrode 1001 is separately positioned with second reference electrode 1002, and first reference electrode 1001 is arranged in the third layer chip 3 Front, the front of the layer 5 chip 5 is arranged in second reference electrode 1002；The working electrode 11 includes first Working electrode 1101 and the second working electrode 1102, first working electrode 1101 are separated with second working electrode 1102 The front of the third layer chip 3 is arranged in setting, first working electrode 1101, and second working electrode 1102 is arranged In the front of the layer 5 chip 5；One end of first working electrode 1101 and first reference electrode 1001 is in institute The front for stating third layer chip 3 is respectively positioned in the sensing chamber 9；First working electrode 1101 and first reference electrode 1001 other end all extends to the first layer chip 1 and the second layer chip 2 in the front of the third layer chip 3 Except the end of one end and form the test side 1 being connected with detecting instrument；Second reference electrode 1002 and described The other end of second working electrode 1102 all extends to the third layer chip 3 and described in the front of the layer 5 chip 5 Except the end of one end of the 4th layer of chip 4 and form the test side 3 1203 being connected with the detecting instrument；The detection Hold 3 1203 exposed except the end of the test side 1, i.e., first layer chip 1 is flushed with second layer chip 2, third Layer chip 3 flushed with the 4th layer of chip 4 and the test side 1 that is formed on third layer chip 3 it is exposed in first layer chip 1 and Except the end of second layer chip 2, the test side 3 1203 formed on layer 5 chip 5 is exposed in third layer chip 3 and the 4th Except the end of layer chip 4；Such layering setting can be to avoid the first working electrode 1101 and first on third layer chip 3 The interference between the second working electrode 1102 and the second reference electrode 1002 on reference electrode 1001 and layer 5 chip 5, and Test side 1 and detection after making the multi-channel fluid hemostasis examination chip insertion detecting instrument with five-layer structure 3 1203 are held can while to be directly connected with detecting instrument；It is corresponding with the mainstream channel 601 multiple described to divide microfluid logical The length of point microfluidic channel 602 described in adjacent two is all different in road 602, multiple described divides microfluid so that adjacent Multiple sensing chamber 9 that channel 602 connects form the setting that is dislocatedly distributed；Such setting can reduce the big of the chip body It is small, reduce cost；The second layer chip 2 is equipped with liquid receiving port 1, and the third layer chip 3 is equipped with liquid receiving port 2 301, institute The 4th layer of chip 4 is stated equipped with liquid receiving port 3 401, the liquid receiving port 1, the liquid receiving port 2 301 and the liquid receiving port 3 401 It is corresponding with the position of the well 8 to be arranged and be connected with the well 8；The mainstream channel 601 includes the One mainstream channel 6011 and the second mainstream channel 6012, it is described that microfluidic channel 602 is divided to include first point of microfluidic channel 6021 With second point of microfluidic channel 6022, first mainstream channel 6011 and first point of microfluidic channel 6021 are through setting On the second layer chip 2, second mainstream channel 6012 and second point of microfluidic channel 6022 exist through setting On the 4th layer of chip 4, one end of first mainstream channel 6011 is connected with the liquid receiving port 1, and the other end is logical Multiple first point of microfluidic channel 6021 are crossed to connect one to one multiple sensing chamber 9；Second mainstream channel 6012 one end is connected with the liquid receiving port 3 401, the other end by multiple second point of microfluidic channel 6022 one by one It is correspondingly connected with multiple sensing chamber 9；After test sample enters chip by well 8, flowed to respectively by liquid receiving port 1 First mainstream channel 6011 and liquid receiving port 2 301, then liquid receiving port 3 401 is flowed to by liquid receiving port 2 301, pass through liquid receiving port three 401 flow to the second mainstream channel 6012, to realize each sensing chamber for flowing to second layer chip 2 simultaneously respectively and the 4th layer of core Each sensing chamber 9 of piece 4；First mainstream channel 6011 prolongs in the horizontal direction to one end far from the liquid receiving port 1 It stretches and is equipped with first point of one connecting detection room one of microfluidic channel, first mainstream channel 6011 is to far from the liquid receiving port one 201 one end vertically extends to two sides respectively is equipped with first point of microfluidic channel two and first point of microfluidic channel three, First point of microfluidic channel two extends to both ends respectively along the direction parallel with first mainstream channel 6011 to be connected with Sensing chamber two and sensing chamber three, first point of microbody channel three are distinguished along the direction parallel with first mainstream channel 6011 Extend to both ends and is connected with sensing chamber four and sensing chamber five；Second mainstream channel 6012 is to far from the liquid receiving port 3 401 One end it is horizontally extending be equipped with second point of microfluidic channel, one connecting detection room six, second mainstream channel 6012 to One end far from the liquid receiving port 3 401 vertically extends to two sides respectively is equipped with second point of microfluidic channel two and second Point microfluidic channel three, second point of microfluidic channel two along the direction parallel with second mainstream channel 6012 respectively to Both ends, which extend, is connected with sensing chamber seven and sensing chamber eight, second point of microbody channel three along with second mainstream channel 6012 Parallel direction extends to both ends respectively is connected with sensing chamber nine and sensing chamber ten；That is the quantity of the sensing chamber 9 of second layer chip 2 It is 5, the quantity of the sensing chamber 9 on the 4th layer of chip 4 is 5, this has the multi-channel fluid hemostasis examination of five-layer structure The quantity of the sensing chamber 9 of chip is 10；The liquid receiving port 1, the liquid receiving port 2 301 and the liquid receiving port 3 401 Size be all larger than or equal to the well 8 size；Multiple gas vents 7 are arranged at and multiple sensing chamber 9 The top of the tangent position in end；The quantity of the first layer chip 1 and the gas vent 7 on the layer 5 chip 5 is equal It is 5；The end of sensing chamber 9 is far from the one end for dividing microfluidic channel 602, each sensing chamber 9 is provided with a gas vent 7；The shape of each test side 12 is that the spacing between rectangle and each test side 12 is equal.

Embodiment 3: this has the multi-channel fluid hemostasis examination chip of five-layer structure, including chip body, the core Piece ontology successively includes first layer chip 1, second layer chip 2, third layer chip 3, the 4th layer of chip 4 and layer 5 from top to bottom Chip 5, the first layer chip 1, second layer chip 2, third layer chip 3, the 4th layer of chip 4 are matched with layer 5 chip 5 Closed fluid channel and multiple mutually independent sensing chamber 9 are defined, the first layer chip 1 is equipped with well 8, described to add Sample hole 8 is connected by the fluid channel 6 with the sensing chamber 9；The chip body further includes electrode, each sensing chamber It is corresponded in 9 and is equipped with the electrode；The second layer chip 2 and the 4th layer of chip 4 are through equipped with the miniflow Road 6 and sensing chamber 9；The fluid channel 6 includes a mainstream channel 601 and multiple points of microfluidic channels 602, the mainstream channel 601 end distributes multiple point microfluidic channels 602, multiple microfluidic channels 602 and multiple mutually indepedent of dividing Sensing chamber 9 correspond connection；The front end of the mainstream channel 601 is connected to the well 8；Each sensing chamber 9 by Point microfluidic channel 602 is extended to form to both ends and to extending out at its end, i.e., the width of sensing chamber 9 is greater than coupled Dividing the width in microbody channel 602, such setting makes detection sample be easier faster flow detection room 9, and in sensing chamber 9 It is embedded with detection reagent in advance；Multiple gas vents 7, Duo Gesuo are equipped on the first layer chip 1 and the layer 5 chip 5 State gas vent 7 be arranged at the first layer chip 1 and the layer 5 chip 5 one end and setting with the sensing chamber 9 It is corresponded at corresponding position and with multiple sensing chamber 9；The electrode is arranged on the third layer chip 3；Institute Stating electrode includes reference electrode 10 and working electrode 11, corresponds in each sensing chamber 9 and is equipped with the reference electrode 10 and the working electrode 11；The working electrode 11 is arranged on the third layer chip 3 with the reference electrode 10；Institute The one end for stating working electrode 11 and the reference electrode 10 is respectively positioned in the sensing chamber 9, and the other end all extends to the third The end of layer chip 3 forms test side 12；With it is described divide it is independent in the corresponding multiple sensing chamber 9 of microfluidic channel 602 The test side 12 equipped with a working electrode 11 and independently is led to, divides microfluidic channel 602 corresponding more with described The reference electrode 10 in a sensing chamber 9 is that the test side 12 is led to after being serially connected；Reference electrode 10 In rectangle and setting is flushed with the end of third layer chip 3 in test side 12 with working electrode 11；The reference electrode 10 wraps Include the first reference electrode 1001 and the second reference electrode 1002, first reference electrode 1001 and second reference electrode 1002 is separately positioned, and the front of the third layer chip 3, second reference electrode is arranged in first reference electrode 1001 1002 are arranged in the back side of the third layer chip 3；The working electrode 11 includes the first working electrode 1101 and the second work Electrode 1102, first working electrode 1101 and second working electrode 1102 are separately positioned, first working electrode 1101 are arranged in the front of the third layer chip 3, and the back of the third layer chip 3 is arranged in second working electrode 1102 Face；One end of first working electrode 1101 and first reference electrode 1001 is equal in the front of the third layer chip 3 In the sensing chamber 9；The other end of first working electrode 1101 and first reference electrode 1001 is described the The front of three layers of chip 3 all extends to except the end of one end of the first layer chip 1 and the second layer chip 2 and is formed The test side 1 being connected with detecting instrument；The one of second reference electrode 1002 and second working electrode 1102 End is respectively positioned in the sensing chamber 9；Second reference electrode 1002 and 1102 other end of the second working electrode are described The back side of third layer chip 3 all extends to except the end of one end of the 4th layer of chip 4 and the layer 5 chip 5 and shape At the test side 2 1202 being connected with the detecting instrument；The end of i.e. described first layer chip 1 and the second layer chip 2 It flushes and is flushed with the end of the 4th layer of chip 4 and layer 5 chip 5, the test side formed on the third layer chip 3 1 and test side 2 1202 it is exposed in the end of first layer chip 1 and second layer chip 2 and the 4th layer of chip 4 and the 5th The end of layer chip 5；It is corresponding with the mainstream channel 601 it is multiple it is described divide in microfluidic channel 602 extend to the same direction Adjacent two described in the length of point microfluidic channel 602 be all different so that adjacent multiple described points of the same direction Multiple sensing chamber 9 that microfluidic channel 602 connects form the setting that is dislocatedly distributed；Such setting can reduce the chip sheet The size of body, reduces cost；The second layer chip 2 is equipped with liquid receiving port 1, and the third layer chip 3 is equipped with liquid receiving port two 301, the 4th layer of chip 4 is equipped with liquid receiving port 3 401, the liquid receiving port 1, the liquid receiving port 2 301 and described connects liquid Mouth 3 401 is corresponding with the position of the well 8 to be arranged and is connected with the well 8；The mainstream channel 601 It is described that microfluidic channel 602 is divided to include that first point of microfluid is logical including the first mainstream channel 6011 and the second mainstream channel 6012 Road 6021 and second point of microfluidic channel 6022, first mainstream channel 6011 and first point of microfluidic channel 6021 are passed through It wears and sets on the second layer chip 2, second mainstream channel 6012 and second point of microfluidic channel 6022 run through It is arranged on the 4th layer of chip 4, one end of first mainstream channel 6011 is connected with the liquid receiving port 1, separately One end is connected one to one multiple sensing chamber 9 by multiple first point of microfluidic channel 6021；Second mainstream The one end in channel 6012 is connected with the liquid receiving port 3 401, and the other end passes through multiple second point of microfluidic channel 6022 Connect one to one multiple sensing chamber 9；After test sample enters chip by well 8, distinguished by liquid receiving port 1 The first mainstream channel 6011 and liquid receiving port 2 301 are flowed to, then liquid receiving port 3 401 is flowed to by liquid receiving port 2 301, passes through liquid receiving port 3 401 flow to the second mainstream channel 6012, to realize each sensing chamber for flowing to second layer chip 2 simultaneously respectively and the 4th layer Each sensing chamber 9 of chip 4；First mainstream channel 6011 to far from the liquid receiving port 1 one end in the horizontal direction Extend and be equipped with first point of one connecting detection room one of microfluidic channel, first mainstream channel 6011 is to far from the liquid receiving port one 201 one end vertically extends to two sides respectively is equipped with first point of microfluidic channel two and first point of microfluidic channel three, First point of microfluidic channel two extends to both ends respectively along the direction parallel with first mainstream channel 6011 to be connected with Sensing chamber two and sensing chamber three, first point of microbody channel three are distinguished along the direction parallel with first mainstream channel 6011 Extend to both ends and is connected with sensing chamber four and sensing chamber five；Second mainstream channel 6012 is to far from the liquid receiving port 3 401 One end it is horizontally extending be equipped with second point of microfluidic channel, one connecting detection room six, second mainstream channel 6012 to One end far from the liquid receiving port 3 401 vertically extends to two sides respectively is equipped with second point of microfluidic channel two and second Point microfluidic channel three, second point of microfluidic channel two along the direction parallel with second mainstream channel 6012 respectively to Both ends, which extend, is connected with sensing chamber seven and sensing chamber eight, second point of microbody channel three along with second mainstream channel 6012 Parallel direction extends to both ends respectively is connected with sensing chamber nine and sensing chamber ten；That is the quantity of the sensing chamber 9 of second layer chip 2 It is 5, the quantity of the sensing chamber 9 on the 4th layer of chip 4 is 5, this has the multi-channel fluid hemostasis examination of five-layer structure The quantity of the sensing chamber 9 of chip is 10；The liquid receiving port 1, the liquid receiving port 2 301 and the liquid receiving port 3 401 Size be all larger than or equal to the well 8 size；Multiple gas vents 7 are arranged at and multiple sensing chamber 9 The top of the tangent position in end；The quantity of the first layer chip 1 and the gas vent 7 on the layer 5 chip 5 is equal It is 5；The end of sensing chamber 9 is far from the one end for dividing microfluidic channel 602, each sensing chamber 9 is provided with a gas vent 7；The shape of each test side 12 is that the spacing between rectangle and each test side 12 is equal；The first layer core Piece 1, second layer chip 2, third layer chip 3, the 4th layer of chip 4 are bonded by way of glued integral with layer 5 chip 5 's；The second layer chip 2 and the 4th layer of chip 4 are pressure sensitive adhesive tape, the first layer chip 1, third layer chip 3 and layer 5 The material of chip 5 is any one of PMMA, PP, PE, PET, and the first layer chip 1 and the layer 5 chip 5 Surface all has hydrophilic film, and sample is made to flow into mainstream channel quickly through the well 8, then be diverted to each point it is micro- Fluid channel 602；The thickness of the second layer chip 2 and the 4th layer of chip 4 is 0.1~1.0mm；The first layer chip 1, Second layer chip 2 and the front of third layer chip 3 and the back side phase of the 4th layer of chip 4, layer 5 chip 5 and third layer chip 3 It is 0.1~1.0mm that cooperation, which defines the depth of closed fluid channel 6, and the width for matching the sensing chamber 9 defined is equal For 1.0~2.0mm；Point microfluidic channel 602 described in each all has nozzle in the junction with the sensing chamber 9；It is described Electrode with a thickness of 0.5mm.

Embodiment 4: this has the multi-channel fluid hemostasis examination chip of five-layer structure, including chip body, the core Piece ontology successively includes first layer chip 1, second layer chip 2, third layer chip 3, the 4th layer of chip 4 and layer 5 from top to bottom Chip 5, the first layer chip 1, second layer chip 2, third layer chip 3, the 4th layer of chip 4 are matched with layer 5 chip 5 Closed fluid channel and multiple mutually independent sensing chamber 9 are defined, the first layer chip 1 is equipped with well 8, described to add Sample hole 8 is connected by the fluid channel 6 with the sensing chamber 9；The chip body further includes electrode, each sensing chamber It is corresponded in 9 and is equipped with the electrode；The second layer chip 2 and the 4th layer of chip 4 are through equipped with the miniflow Road 6 and sensing chamber 9；The fluid channel 6 includes a mainstream channel 601 and multiple points of microfluidic channels 602, the mainstream channel 601 end distributes multiple point microfluidic channels 602, multiple microfluidic channels 602 and multiple mutually indepedent of dividing Sensing chamber 9 correspond connection；The front end of the mainstream channel 601 is connected to the well 8；Each sensing chamber 9 by Point microfluidic channel 602 is extended to form to both ends and to extending out at its end, i.e., the width of sensing chamber 9 is greater than coupled Dividing the width in microbody channel 602, such setting makes detection sample be easier faster flow detection room 9, and in sensing chamber 9 It is embedded with detection reagent in advance；Multiple gas vents 7, Duo Gesuo are equipped on the first layer chip 1 and the layer 5 chip 5 State gas vent 7 be arranged at the first layer chip 1 and the layer 5 chip 5 one end and setting with the sensing chamber 9 It is corresponded at corresponding position and with multiple sensing chamber 9；Electrode setting is in the third layer chip 3 and described On layer 5 chip 5；The electrode includes reference electrode 10 and working electrode 11, is corresponded in each sensing chamber 9 Equipped with the reference electrode 10 and the working electrode 11；The working electrode 11 is arranged at described with the reference electrode 10 On third layer chip 3 and the layer 5 chip 5；One end of the working electrode 11 and the reference electrode 10 is respectively positioned on described In sensing chamber 9, the other end all extends to the third layer chip 3 and the end of the layer 5 chip 5 forms test side 12；With It is independent in the corresponding multiple sensing chamber 9 of described point of microfluidic channel 602 to be equipped with the working electrode 11 simultaneously independently The test side 12 is led to, with the reference electrode divided in the corresponding multiple sensing chamber 9 of microfluidic channel 602 10 be that the test side 12 is led to after being serially connected；Reference electrode 10 is in rectangle in test side 12 with working electrode 11 And setting is flushed with the end of third layer chip 3 or/and layer 5 chip 5；The reference electrode 10 includes the first reference electrode 1001 and second reference electrode 1002, first reference electrode 1001 and second reference electrode 1002 are separately positioned, institute The front that the third layer chip 3 is arranged in the first reference electrode 1001 is stated, second reference electrode 1002 is arranged described The front of layer 5 chip 5；The working electrode 11 includes the first working electrode 1101 and the second working electrode 1102, and described the One working electrode 1101 and second working electrode 1102 are separately positioned, and first working electrode 1101 setting is described the The front of the layer 5 chip 5 is arranged in the front of three layers of chip 3, second working electrode 1102；First work One end of electrode 1101 and first reference electrode 1001 is respectively positioned on the sensing chamber 9 in the front of the third layer chip 3 It is interior；The other end of first working electrode 1101 and first reference electrode 1001 is in the front of the third layer chip 3 It all extends to except the end of one end of the first layer chip 1 and the second layer chip 2 and is formed and is connected with detecting instrument The test side 1 connect；The other end of second reference electrode 1002 and second working electrode 1102 is the described 5th Except the end of front one end for all extending to the third layer chip 3 and the 4th layer of chip 4 of layer chip 5 and formed with The test side 3 1203 that the detecting instrument is connected；The exposed end in the test side 1 in the test side 3 1203 Except；I.e. first layer chip 1 is flushed with second layer chip 2, and third layer chip 3 is flushed with the 4th layer of chip 4 and third layer chip 3 The test side 1 of upper formation is exposed except the end of first layer chip 1 and second layer chip 2, shape on layer 5 chip 5 At test side 3 1203 it is exposed except the end of third layer chip 3 and the 4th layer of chip 4；Such setting can be to avoid The first working electrode 1101 and the first reference electrode 1001 on three layers of chip 3 and the second working electrode on layer 5 chip 5 1102 and the second interference between reference electrode 1002, and make the multi-channel fluid hemostasis examination chip with five-layer structure Test side 1 after insertion detecting instrument can directly be connected with detecting instrument simultaneously with test side 3 1203；With the master Divide miniflow described in corresponding multiple adjacent two being divided in microfluidic channel 602 to same direction extension of circulation road 601 The length in body channel 602 is all different, so that multiple described to divide microfluidic channel 602 to connect multiple for the same direction adjacent The sensing chamber 9 forms the setting that is dislocatedly distributed；Such setting can reduce the size of the chip body, reduce cost；It is described Second layer chip 2 is equipped with liquid receiving port 1, and the third layer chip 3 is equipped with liquid receiving port 2 301, and the 4th layer of chip 4 is equipped with Liquid receiving port 3 401, the liquid receiving port 1, the liquid receiving port 2 301 and the liquid receiving port 3 401 with the well 8 Position is corresponding to be arranged and is connected with the well 8；The mainstream channel 601 includes the first mainstream channel 6011 and the Two mainstream channels 6012, it is described that microfluidic channel 602 is divided to include first point of microfluidic channel 6021 and second point of microfluidic channel 6022, first mainstream channel 6011 and first point of microfluidic channel 6021 run through setting in the second layer chip 2 On, second mainstream channel 6012 and second point of microfluidic channel 6022 run through setting on the 4th layer of chip 4, One end of first mainstream channel 6011 is connected with the liquid receiving port 1, and the other end is micro- by multiple described first points Fluid channel 6021 connects one to one multiple sensing chamber 9；One end of second mainstream channel 6012 connects liquid with described Mouthfuls 3 401 are connected, and the other end is connected one to one multiple detections by multiple second point of microfluidic channel 6022 Room 9；After test sample enters chip by well 8, the first mainstream channel 6011 is flowed to by liquid receiving port 1 respectively and is connect Liquid mouth 2 301, then liquid receiving port 3 401 is flowed to by liquid receiving port 2 301, the second mainstream channel is flowed to by liquid receiving port 3 401 6012, each sensing chamber of second layer chip 2 and each sensing chamber 9 of the 4th layer of chip 4 are flowed to simultaneously respectively to realize；Institute Stating the first mainstream channel 6011, horizontally extending to be equipped with first point of microfluid logical to one end far from the liquid receiving port 1 One connecting detection room one of road, first mainstream channel 6011 vertically divide to one end far from the liquid receiving port 1 Do not extend to two sides and is equipped with first point of microfluidic channel two and first point of microfluidic channel three, first point of microfluidic channel two Extend respectively to both ends along the direction parallel with first mainstream channel 6011 and is connected with sensing chamber two and sensing chamber three, it is described First point of microbody channel three extends to both ends respectively along the direction parallel with first mainstream channel 6011 is connected with sensing chamber Four and sensing chamber five；Second mainstream channel 6012 is set to one end far from the liquid receiving port 3 401 is horizontally extending There is second point of microfluidic channel, an one connecting detection room six, second mainstream channel 6012 is to far from the liquid receiving port 3 401 One end vertically extends to two sides respectively is equipped with second point of microfluidic channel two and second point of microfluidic channel three, and described the Two points of microfluidic channels two extend to both ends respectively along the direction parallel with second mainstream channel 6012 is connected with sensing chamber Seven and sensing chamber eight, second point of microbody channel three is along the direction parallel with second mainstream channel 6012 respectively to both ends Extension is connected with sensing chamber nine and sensing chamber ten；I.e. the quantity of the sensing chamber 9 of second layer chip 2 is 5, on the 4th layer of chip 4 Sensing chamber 9 quantity be 5, this has the quantity of the sensing chamber 9 of the multi-channel fluid hemostasis examination chip of five-layer structure It is 10；The size of the liquid receiving port 1, the liquid receiving port 2 301 and the liquid receiving port 3 401 is all larger than or is equal to described The size of well 8；Multiple gas vents 7 are arranged at the top of the position tangent with the end of multiple sensing chamber 9； The quantity of the first layer chip 1 and the gas vent 7 on the layer 5 chip 5 is 5；The end of sensing chamber 9 is Far from the one end for dividing microfluidic channel 602, each sensing chamber 9 is provided with a gas vent 7；The shape of each test side 12 Shape is that the spacing between rectangle and each test side 12 is equal；The first layer chip 1, second layer chip 2, third layer Chip 3, the 4th layer of chip 4 are bonded by way of glued integral with layer 5 chip 5；The second layer chip 2 and Four layers of chip 4 are pressure sensitive adhesive tape, the material of the first layer chip 1, third layer chip 3 and layer 5 chip 5 be PMMA, Any one of PP, PE, PET, and the surface of the first layer chip 1 and the layer 5 chip 5 all has hydrophilic film, makes sample This flows into mainstream channel quickly through the well 8, then is diverted to each point of microfluidic channel 602；Described second The thickness of layer chip 2 and the 4th layer of chip 4 is 0.1~1.0mm；The first layer chip 1, second layer chip 2 and third layer The front of chip 3 and the 4th layer of chip 4, layer 5 chip 5 match with the back side of third layer chip 3 and define closed miniflow The depth in road 6 is 0.1~1.0mm, and the width for matching the sensing chamber 9 defined is 1.0~2.0mm；Each Described point of microfluidic channel 602 all has nozzle in the junction with the sensing chamber 9；The electrode with a thickness of 0.5mm.

When specifically used:

Test sample, which is injected, after test sample enters chip by well 8 to well 8 passes through one 201 points of liquid receiving port The first mainstream channel 6011 and liquid receiving port 2 301 are not flowed to, and liquid receiving port 3 401 is flowed to by liquid receiving port 2 301, passes through liquid receiving port 3 401 flow to the second mainstream channel 6012, to realize each sensing chamber for flowing to second layer chip 2 simultaneously respectively and the 4th layer Each sensing chamber 9 of chip 4；The detection reagent embedded in advance in sample and sensing chamber 9 is reacted, while in the first work electricity Under pole 1101 and the effect of the first reference electrode 1001 and the second working electrode 1102 and the second reference electrode 1002, using alternating current Resistance method, the electrochemical signals variation for cooperating mating detecting instrument monitoring reaction to generate, obtains testing result；In this way can once into Sample detects 10 coagulation indexes simultaneously, improves detection efficiency.

The basic principles and main features and advantage of the utility model have been shown and described above.The technical staff of the industry It should be appreciated that the present utility model is not limited to the above embodiments, the above embodiments and description only describe this The principle of utility model, on the premise of not departing from the spirit and scope of the utility model, the utility model also has various change And improvement, such as well shape and structure do some other micro- summaries adjustment or each sensing chamber arrangement mode and Quantity does some adjustment, and these various changes and improvements fall within the scope of the claimed invention.The requires of the utility model Protection scope is defined by the appending claims and its equivalent thereof.

Claims

1. a kind of multi-channel fluid hemostasis examination chip with five-layer structure, including chip body, which is characterized in that described Chip body successively includes first layer chip, second layer chip, third layer chip, the 4th layer of chip and layer 5 core from top to bottom Piece, the first layer chip, second layer chip, third layer chip, the 4th layer of chip match with layer 5 chip and define envelope The fluid channel closed and multiple mutually independent sensing chamber, the first layer chip are equipped with well, and the well passes through institute Fluid channel is stated to be connected with the sensing chamber；The chip body further includes electrode, and each detection is indoor to be corresponded Equipped with the electrode.

2. the multi-channel fluid hemostasis examination chip according to claim 1 with five-layer structure, which is characterized in that institute It states second layer chip and the 4th layer of chip runs through equipped with the fluid channel and sensing chamber；The fluid channel includes a master Circulation road and multiple points of microfluidic channels, the end of the mainstream channel distribute multiple point microfluidic channels, Duo Gesuo Point microfluidic channel is stated to be connected to multiple mutually independent sensing chamber's one-to-one correspondence；The front end of the mainstream channel and the sample-adding Hole connection.

3. the multi-channel fluid hemostasis examination chip according to claim 2 with five-layer structure, which is characterized in that institute It states and is equipped with multiple gas vents on first layer chip and the layer 5 chip, multiple gas vents are arranged at described first Layer chip and the layer 5 chip one end and be arranged at position corresponding with the sensing chamber and with multiple inspections Room is surveyed to correspond.

4. the multi-channel fluid hemostasis examination chip according to claim 3 with five-layer structure, which is characterized in that institute Electrode is stated to be arranged on the third layer chip or be arranged on the third layer chip and the layer 5 chip；The electrode Including reference electrode and working electrode, indoor correspond of each detection is equipped with the reference electrode and work electricity Pole.

5. the multi-channel fluid hemostasis examination chip according to claim 4 with five-layer structure, which is characterized in that institute It states working electrode and the reference electrode is arranged on the third layer chip, or be arranged at the third layer chip and institute It states on layer 5 chip；One end of the working electrode and the reference electrode is respectively positioned on the detection interior, and the other end prolongs Extend to the end of the third layer chip or the end formation test side of the third layer chip and the layer 5 chip；With institute The corresponding multiple detections interiors of point microfluidic channel are stated independently to be equipped with a working electrode and independently lead to institute Test side is stated, multiple indoor reference electrodes of detection corresponding with described point of microfluidic channel are to be serially connected After lead to the test side.

6. the multi-channel fluid hemostasis examination chip according to claim 5 with five-layer structure, which is characterized in that institute Stating reference electrode includes the first reference electrode and the second reference electrode, and first reference electrode and second reference electrode divide From setting, the front of the third layer chip is arranged in first reference electrode, and second reference electrode is arranged described The back side of third layer chip or the front of the layer 5 chip.

7. the multi-channel fluid hemostasis examination chip according to claim 6 with five-layer structure, which is characterized in that institute Stating working electrode includes the first working electrode and the second working electrode, and first working electrode and second working electrode divide From setting, the front of the third layer chip is arranged in first working electrode, and second working electrode is arranged described The back side of third layer chip or the front of the layer 5 chip.

8. the multi-channel fluid hemostasis examination chip according to claim 7 with five-layer structure, which is characterized in that institute The one end for stating the first working electrode and first reference electrode is respectively positioned on the sensing chamber in the front of the third layer chip It is interior；The other end of first working electrode and first reference electrode all extends to institute in the front of the third layer chip It states except the end of one end of first layer chip and the second layer chip and forms the test side one being connected with detecting instrument.

9. the multi-channel fluid hemostasis examination chip according to claim 8 with five-layer structure, which is characterized in that institute The one end for stating the second reference electrode and second working electrode is respectively positioned on the detection interior；Second reference electrode and institute It states the second working electrode other end and all extends to the 4th layer of chip and the layer 5 at the back side of the third layer chip Except the end of one end of chip and formed the test side two that is connected with the detecting instrument or second reference electrode and The other end of second working electrode all extends to the third layer chip and described in the front of the layer 5 chip Except the end of one end of four layers of chip and form the test side three being connected with the detecting instrument.

10. the multi-channel fluid hemostasis examination chip according to claim 9 with five-layer structure, which is characterized in that The test side three is exposed except the end of the test side one.

11. the multi-channel fluid hemostasis examination chip according to claim 2 with five-layer structure, which is characterized in that It is corresponding with the mainstream channel multiple described to be divided to the length of point microfluidic channel described in adjacent in microfluidic channel two equal It is not identical, so that multiple sensing chamber of adjacent multiple described point of microfluidic channel connections form the setting that is dislocatedly distributed.

12. the multi-channel fluid hemostasis examination chip according to claim 9 with five-layer structure, which is characterized in that The second layer chip is equipped with liquid receiving port one, and the third layer chip is equipped with liquid receiving port two, and the 4th layer of chip is equipped with and connects liquid Mouthfuls three, the setting corresponding with the position of the well of the liquid receiving port one, the liquid receiving port two and the liquid receiving port three and It is connected with the well；The mainstream channel includes the first mainstream channel and the second mainstream channel, described to divide microfluid Channel includes first point of microfluidic channel and second point of microfluidic channel, first mainstream channel and first point of microfluid Channel is through being arranged on the second layer chip, and second mainstream channel and second point of microfluidic channel are through setting On the 4th layer of chip, one end of first mainstream channel is connected with the liquid receiving port one, and the other end passes through multiple First point of microfluidic channel connects one to one multiple sensing chamber；One end of second mainstream channel connects with described Liquid mouth three is connected, and the other end is connected one to one multiple sensing chamber by multiple second point of microfluidic channel.

13. the multi-channel fluid hemostasis examination chip according to claim 12 with five-layer structure, which is characterized in that First mainstream channel is equipped with first point of microfluidic channel one to one end far from the liquid receiving port one is horizontally extending Connecting detection room one, first mainstream channel vertically extend to two sides respectively to one end far from the liquid receiving port one Equipped with first point of microfluidic channel two and first point of microfluidic channel three, first point of microfluidic channel two is along with described first The parallel direction of mainstream channel extends to both ends respectively is connected with sensing chamber two and sensing chamber three, first point of microbody channel three Extend respectively to both ends along the direction parallel with first mainstream channel and is connected with sensing chamber four and sensing chamber five.

14. the multi-channel fluid hemostasis examination chip according to claim 12 with five-layer structure, which is characterized in that Second mainstream channel is equipped with second point of microfluidic channel one to one end far from the liquid receiving port three is horizontally extending Connecting detection room six, second mainstream channel vertically extend to two sides respectively to one end far from the liquid receiving port three Equipped with second point of microfluidic channel two and second point of microfluidic channel three, second point of microfluidic channel two is along with described second The parallel direction of mainstream channel extends to both ends respectively is connected with sensing chamber seven and sensing chamber eight, second point of microbody channel three Extend respectively to both ends along the direction parallel with second mainstream channel and is connected with sensing chamber nine and sensing chamber ten.

15. according to claim 1 with the multi-channel fluid hemostasis examination chip of five-layer structure, feature described in 3 or 14 It is, the size of the liquid receiving port one, the liquid receiving port two and the liquid receiving port three is all larger than or big equal to the well It is small；Multiple gas vents are arranged at the top of the position tangent with the end of multiple sensing chamber；The first layer core The quantity of piece and the gas vent on the layer 5 chip is 5.