CN208554242U - A kind of multichannel quickly detects microfluid detection chip - Google Patents
A kind of multichannel quickly detects microfluid detection chip Download PDFInfo
- Publication number
- CN208554242U CN208554242U CN201820904218.3U CN201820904218U CN208554242U CN 208554242 U CN208554242 U CN 208554242U CN 201820904218 U CN201820904218 U CN 201820904218U CN 208554242 U CN208554242 U CN 208554242U
- Authority
- CN
- China
- Prior art keywords
- chip
- sensing chamber
- channel
- electrode
- middle layer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The utility model discloses a kind of multichannels quickly to detect microfluid detection chip, including chip body, chip thief hatch, multiple mutually independent sensing chamber and fluid channel are provided on chip body, chip thief hatch is connected to by fluid channel with sensing chamber, chip body further includes electrode, and sensing chamber is connected with electrode；Fluid channel includes a mainstream channel and multiple points of microfluidic channels, and the end of mainstream channel distributes multiple points of microfluidic channels, and multiple points of microfluidic channels are corresponded with multiple mutually independent sensing chamber and are connected to；The other end of mainstream channel is connected to chip thief hatch.By design mainstream channel and multiple points of microfluidic channels, for the flowing of guide blood sample, so that a sample chamber, can inject sample and non-contaminated samples, easy sample introduction to multiple reaction chambers simultaneously, while detecting multiple samples, have multichannel effect；Chip structure is simple, easy to operate, improves detection efficiency, reduces the consumption of resource, reduces costs.
Description
Technical field
The utility model belongs to technical field of medical equipment, quickly detects microfluid detection more particularly, to a kind of multichannel
Chip.
Background technique
Microfluidics be across include engineering science, physics, chemistry, microtechnology and biotechnology diverse discipline apply
Technology.Microfluidics be related to research to micro fluid and to how such as micro-fluid chip etc various microfluids
The research of such a small amount of fluid is manipulated, controlled and used in system and equipment.Such as: microfluid biochip (referred to as " core
Piece laboratory ") it is operated in molecular biology field for integrating chemical examination, to be used for such as enzyme analysis and DNA, detect bioid
The purpose of learning toxin and pathogen, diagnosing the illness.
Micro-fluidic chip (microfluidic chip) is current micro-total analysis system (Miniaturized Total
Analysis Systems) development hot fields.Microfluidic chip analysis is using chip as operating platform, while with analytical chemistry
Based on, it is to rely on micro electro mechanical processing technology, is main application at present with life science using microchannel network as structure feature
Object is the emphasis of current micro-total analysis system field development.Its target is the function entire laboratory, including sampling,
Dilution, reagent adding, reaction, separation, detection etc. are integrated on microchip.Micro-fluidic chip is the main flat of microflow control technique realization
Platform.Its device characteristic is mainly that its resulting structure (channel, sensing chamber and other certain functional components) for accommodating fluid at least exists
It is micron order scale on one latitude.Due to micron-sized structure, fluid shows and produces wherein different from macro-scale
Property.Therefore the performance that unique analysis generates is developed.The characteristics of micro-fluidic chip and advantages for development: micro-fluidic core
Piece has the characteristics that liquid flowing controllable, consumption sample and reagent are few, improve to analysis speed tenfold hundreds of times, it can be with
Analysis while carrying out a sample up to a hundred within a few minutes or even shorter time, and can be with the pretreatment of canbe used on line sample
And analysis overall process.Its application purpose generated is to realize ultimate aim-chip lab of micro-total analysis system, at present work
The focus on the application field for making to develop is life science.
Current international research status: innovation focuses mostly in terms of separation, detection architecture；To how introducing practical sample on chip
The problems of product analysis, such as sample is introduced, is varied, the related research of pre-treatment is also very weak.Its development is dependent on more
The development of subject crossing.
In Chinese patent literature CN205361375U, a kind of micro-fluid chip is disclosed, is successively repeatedly connected to from the bottom up
Glass substrate layer, middle layer and upper cap rock, the glass substrate layer, middle layer are matched with upper cap rock defines closed annular
Fluid channel and sensing chamber, the fluid channel be located at the outside of sensing chamber, and be connected to sensing chamber, the upper cap rock side is equipped with
The fluid-infusing port being connected to fluid channel, the upper cap rock are equipped with multiple gas vents in the other end of fluid channel.But above-mentioned technology
Scheme detection flux is small, and it is at high cost that structure is complicated, and injection port designs unreasonable easy cause sample contamination.
Therefore, it is necessary to which the injection port for developing a kind of rational design avoids sample contaminated and detection flux is big, detects effect
Rate and the high multichannel of accuracy quickly detect microfluid detection chip.
Utility model content
Avoid sample contaminated the technical problem to be solved by the present invention is to provide a kind of injection port of rational design and
The multichannel that detection flux is big, detection efficiency and accuracy are high quickly detects microfluid detection chip.
In order to solve the above technical problems, the technical solution adopted in the utility model is, which quickly detects microfluid
Detection chip, including chip body are provided with chip thief hatch, multiple mutually independent sensing chamber and micro- on the chip body
Runner, the chip thief hatch are connected to by fluid channel with the sensing chamber, and the chip body further includes electrode, the detection
Room is connected with the electrode；The fluid channel includes a mainstream channel and multiple points of microfluidic channels, the mainstream channel
End distribute multiple point microfluidic channels, it is multiple described to divide microfluidic channel and multiple mutually independent sensing chamber one
One corresponding connection；The other end of the mainstream channel is connected to the chip thief hatch.
By adopting the above technical scheme, micro-fluidic chip detection has precision high, and speed is fast, the lower feature of testing cost,
It is appropriate for the detection that accurate medical rings carry out, it is logical by the mainstream channel and multiple points of microfluids that design specific structure form
Road so that a sample chamber, can inject sample and not to multiple reaction chambers simultaneously for the flowing of guide blood sample
Contaminated samples, easy sample introduction；After the sampling of chip thief hatch, multiple points of microfluidic channels are flowed to by mainstream channel while respectively, then into
Enter multiple mutually independent sensing chamber, detection interior is embedded with detection reagent in advance, may be implemented to detect multiple samples simultaneously in this way
This, has the effect of multichannel；Chip structure is simple, easy to operate, improves detection efficiency, and greatly reduces disappearing for resource
Consumption；It realizes quickly detection, reduces costs.
The utility model further improvement lies in that, the chip body from the bottom up successively include backplane level, middle layer and
Upper cap rock, the backplane level, middle layer are matched with upper cap rock defines closed fluid channel and multiple mutually independent detections
Room；The fluid channel and sensing chamber are in the middle layer；The upper cap rock is equipped with liquid injection port and multiple gas vents, described more
A gas vent is arranged in the side of the upper cap rock and is arranged at position corresponding with the end of the fluid channel, the liquid
Body inlet is connected with the front end of the fluid channel；The backplane level is equipped with electrode, the sensing chamber and the electrode phase
Connection.Using the chip of the three-decker of backplane level, middle layer and upper cap rock, design is rationally, simple and compact for structure, and reduces
Cost, the chip thief hatch with easy sample introduction；By the way that multiple gas vents are arranged in upper cover, so that the flow resistance of fluid to be measured subtracts
Small, flowing more rapidly, realizes Fast Filling sensing chamber；The flowing of gas vent being provided with conducive to sample, facilitates sample introduction, if not having
Gas vent is set, then sample cannot flow into sensing chamber and be reacted, the indoor embedding detection reagent in advance of chip detection.
The utility model further improvement lies in that, multiple mutually independent fan-shaped distributions of sensing chamber, by the major flow
The end in road is split into multiple points of microfluidic channels and is connected to multiple mutually independent sensing chamber again.By designing specific structure shape
The mainstream channel of formula and multiple points of microfluidic channels, for the flowing of guide blood sample, so that a sample chamber, Neng Goutong
When to multiple reaction chambers inject sample so that flowing is faster, improve detection efficiency.
The utility model further improvement lies in that, the chip thief hatch is made of liquid injection port, chip sampling
Mouth is connected to the mainstream channel, and one end of the mainstream channel is provided at position corresponding with the liquid injection port
Liquid receiving port；The other end of the mainstream channel connects all point microfluidic channels.It is easy using the chip thief hatch of this structure
It is contaminated in sampling, and not, structure is simple, at low cost.
The utility model further improvement lies in that, the backplane level, middle layer matched with upper cap rock define it is closed
Fluid channel, sensing chamber and funnel area；The side of the lower end of the backplane level is equipped with a notch, the liquid injection port, funnel
Region and notch are separately positioned at corresponding position and of different sizes on cap rock, middle layer and backplane level；The chip
Thief hatch is made of liquid injection port, funnel area and notch together and by the bottom phase of the fluid channel and the sensing chamber
Connection.Chip thief hatch is arranged to funnel-like, base areas is big, and top cover area is small, middle layer funnel, such structure setting
Rationally and structure is simple, makes sample be easier to enter, and be not contaminated, improves the efficiency of detection.
The utility model further improvement lies in that, the liquid injection port, funnel area and notch are arc and radian
It is different；The liquid injection port, funnel area are semicircular arc, and the radius of the funnel area is infused not less than the liquid
The arc radius of entrance；The funnel area by curved mainstream channel disperse out multiple points of microfluidic channels with it is the multiple
Mutually independent sensing chamber is connected to one by one；The area of the notch is less than the area of the funnel area；
Or the mainstream channel is funnel area, the liquid injection port is arc, with funnel area part area
Domain overlapping；It is horn-like that the funnel area has opening toe-in to be formed, and the funnel area is inwardly divided at its tail end
Shed multiple points of microfluidic channels, is connected one to one multiple mutually independent detections by multiple described point of microfluidic channels
Room.Wherein liquid injection port is semicircular arc, area under the same conditions, the sample size of this structure setting injection is most,
And the radius of funnel area is not less than the arc radius of the liquid injection port, infuses so that funnel area can be accommodated all from liquid
The sample liquid of entrance injection, will not there is loss；Setting curved channel is unlikely to cause so that sample flows slowly into sensing chamber
Sensing chamber's atmospheric pressure is uprushed.
It wherein injects liquid into mouth and is set as arc, it is Chong Die with the funnel area partial region；The funnel area
There is opening toe-in to be formed horn-like so that sample gradually flows inward into, is not stopped in opening, avoid sample from being lost, example
It is fast to realize blood sample at 1 second or so for the speed for such as entering thief hatch using the funnel area blood sample of this structure
In fast inspiration thief hatch；The effect that notch is arranged is fitted with finger tripe, and sampling is facilitated.
The utility model further improvement lies in that, the backplane level, middle layer and upper cap rock are double by passing through middle layer
The mode of face gluing is bonded integrally.
As the optimal technical scheme of the utility model, the middle layer is pressure sensitive adhesive tape, the upper cap rock and/or described
The material of backplane level is any one of PMMA, PP, PE, PET, and the surface of the upper cap rock and the backplane level all has parent
Moisture film makes sample flow into mainstream channel quickly through the chip thief hatch, then is diverted to each point of microfluidic channel.
It adopts this technical solution, material is easy to get, and the manufacture craft of pressure sensitive adhesive tape can be with its thickness of the control of precision, so using this skill
Art scheme, can be accurately controlled the depth and size of fluid channel, while be also convenient for the depth of control sensing chamber, so that microfluid
The thickness deviation of each sensing chamber of chip is small, and consistency is high, improves the accuracy of detection；The surface of upper cap rock and backplane level
It is respectively provided with hydrophilic film, sample can be made to pass through chip thief hatch more quickly and flow into mainstream channel and be diverted to each point of microfluid
Channel, in this way quickening flowing velocity, can be improved detection efficiency.
As the optimal technical scheme of the utility model, the middle layer with a thickness of 0.1~1.0mm；The backplane level
Surface be it is flat, the backplane level, middle layer matched with upper cap rock define closed fluid channel depth be 0.1~
1.0mm, the width for matching the sensing chamber defined is 1.0~2.0mm.
Divide microfluidic channel in the company with the sensing chamber as the optimal technical scheme of the utility model, described in each
The place of connecing all has nozzle, and a point microfluidic channel described in each has corresponding electrode, and each electrode includes that input is high
Lateral electrode and input downside electrode, the electrode with a thickness of 50um.It is equipped in the junction of point microfluidic channel and sensing chamber
Nozzle is to make sample more easily flow into sensing chamber more quickly；The effect of electrode is to apply pulse voltage while receiving detection
The signal that reaction of blood generates in room；Electrode end is inserted into detecting instrument, and the electrochemical signals generated by detection reaction are matched
It closes mating detecting instrument and obtains testing result；Electrode end is that the backplane level, middle layer and upper cap rock are bonded to phase after one
For the upper cap rock and the exposed part of middle layer, electrode end is easier and is easily inserted into detector
In device.
Compared with prior art, which quickly detects the master that microfluid detection chip passes through design specific structure form
Circulation road and multiple points of microfluidic channels, for the flowing of guide blood sample, so that a sample chamber, it can be simultaneously to more
A reaction chamber injects sample and non-contaminated samples, easy sample introduction；After the sampling of chip thief hatch, flowed to respectively simultaneously by mainstream channel
Multiple points of microfluidic channels enter back into multiple mutually independent sensing chamber, may be implemented to detect multiple samples simultaneously in this way, have
The effect of multichannel；Chip structure is simple, easy to operate, improves detection efficiency and precision, and greatly reduces disappearing for resource
Consumption；It realizes quickly detection, reduces costs.
Detailed description of the invention
It is further described with reference to the accompanying drawing with the embodiments of the present invention:
Fig. 1 is the planar structure schematic diagram that the utility model multichannel quickly detects microfluid detection chip embodiment 1；
Fig. 2 is the schematic perspective view that the utility model multichannel quickly detects microfluid detection chip embodiment 1；
Fig. 3 is the overall structure figure that the utility model multichannel quickly detects microfluid detection chip embodiment 1；
Fig. 4 is the planar structure schematic diagram that the utility model multichannel quickly detects microfluid detection chip embodiment 2；
Fig. 5 is the schematic perspective view that the utility model multichannel quickly detects microfluid detection chip embodiment 2；
Fig. 6 is the overall structure figure that the utility model multichannel quickly detects microfluid detection chip embodiment 2；
Fig. 7 is the planar structure schematic diagram that the utility model multichannel quickly detects microfluid detection chip embodiment 3；
Fig. 8 is the schematic perspective view that the utility model multichannel quickly detects microfluid detection chip embodiment 3；
Fig. 9 is the overall structure figure that the utility model multichannel quickly detects microfluid detection chip embodiment 3；
Wherein: 1- backplane level；2- middle layer；The upper cap rock of 3-；4- electrode；401- electrode end；5- fluid channel；501- mainstream
Channel；502- divides microfluidic channel；6- gas vent；7- chip thief hatch；701- liquid injection port；702- liquid receiving port；8- detection
Room；9- funnel area；10- notch.
Specific embodiment
Embodiment 1: the multichannel quickly detects microfluid detection chip, including chip body, sets on the chip body
It is equipped with chip thief hatch 7, multiple mutually independent sensing chamber 8 and fluid channel 5, the chip thief hatch 7 passes through fluid channel 5 and institute
The connection of sensing chamber 8 is stated, the chip body further includes electrode 4, and the sensing chamber 8 is connected with the electrode 4；The fluid channel
5 include a mainstream channel 501 and 5 points of microfluidic channels 502, the end of the mainstream channel 501 distribute 5 described in point
Divide the mutually independent sensing chamber 8 of microfluidic channel 502 and 5 to correspond described in microfluidic channel 502,5 to be connected to；The master
The other end of circulation road 501 is connected to the chip thief hatch 7；The chip body from the bottom up successively include backplane level 1, in
Interbed 2 and upper cap rock 3, the backplane level 1, middle layer 2 are matched with upper cap rock 3 defines closed fluid channel 5 and multiple phases
Mutual independent sensing chamber 8；The fluid channel 5 and sensing chamber 8 are in the middle layer 2；The upper cap rock 3 is equipped with liquid injection port
701 and 5 gas vents 6,5 gas vents 6 are arranged in the side of the upper cap rock and the end with the fluid channel 5 are arranged in
It holds at corresponding position, the liquid injection port 701 is connected with the front end of the fluid channel 5；The backplane level 1 is equipped with
Electrode 4, the sensing chamber 8 are connected with the electrode 4；The flowing of gas vent 6 being provided with conducive to sample, facilitates sample introduction, if
It is not provided with 6 sample of gas vent and cannot flow into sensing chamber 8 and reacted, embed detection reagent in advance in the sensing chamber 8 of chip；5
The fan-shaped distribution of a mutually independent sensing chamber 8 is split into 5 points of microfluidic channels 502 by the end of the mainstream channel 501
It is connected to 5 mutually independent sensing chamber 8 again；The backplane level 1, middle layer 2 and upper cap rock 3 pass through 2 Double-faced binded of middle layer
Mode be bonded integrally；The middle layer 2 is pressure sensitive adhesive tape, and the material of the upper cap rock 3 and/or the backplane level 1 is
Any one of PMMA, PP, PE, PET, and the surface of upper cap rock 3 and backplane level 1 has hydrophilic film, makes sample quickly through institute
It states chip thief hatch 7 and flows into mainstream channel 501, then be diverted to each point of microfluidic channel 502；The middle layer 2
With a thickness of 0.1~1.0mm；The surface of the backplane level 1 be it is flat, the backplane level 1, middle layer 2 match boundary with upper cap rock 3
Make closed fluid channel 5 depth be 0.1~1.0mm, match the sensing chamber 8 defined width be 1.0~
2.0mm；Point microfluidic channel 502 described in each all has nozzle, and each institute in the junction with the sensing chamber 8
Stating point microfluidic channel 502 all has corresponding electrode 4, and each electrode 4 includes inputting high lateral electrode and input downside electrode, institute
State electrode 4 with a thickness of 50um；The effect of electrode 4 is to apply pulse voltage while receiving the letter that reaction of blood generates in sensing chamber
Number；Electrode end 401 is inserted into detecting instrument, and the electrochemical signals generated by detection reaction cooperate mating detecting instrument to obtain
Testing result out；Electrode end 401 is after the backplane level 1, middle layer 2 are bonded integrally with upper cap rock 3 relative on described
Cap rock 3 and the exposed part of middle layer 2 enable electrode end 401 to be easier and are easily inserted into detecting instrument,
Testing result out；Wherein as shown in Figures 1 to 3, the chip thief hatch 7 is liquid injection port 701, the chip thief hatch 7
It is connected to the mainstream channel 501, one end of the mainstream channel 501 is in position corresponding with the liquid injection port 701
Place is provided with liquid receiving port 702；The other end of the mainstream channel 501 connects all point microfluidic channels 502.
Embodiment 2: difference from Example 1 is that the structure of chip thief hatch 7 is different, the backplane level 1, middle layer
2 match with upper cap rock 3 and define closed fluid channel 5, sensing chamber 8 and funnel area 9；The one of the lower end of the backplane level 1
Side is equipped with a notch 10, and the liquid injection port 701, funnel area 9 and notch 10 are separately positioned on cap rock 3,2 and of middle layer
It is at corresponding position and of different sizes on backplane level 1；The chip thief hatch 7 is by liquid injection port 701,9 and of funnel area
Notch 10 constitutes together and is connected by the fluid channel 5 with the bottom of the sensing chamber 8；Specifically: the multichannel is quick
Detect microfluid detection chip, including chip body is provided with chip thief hatch 7, multiple mutually indepedent on the chip body
Sensing chamber 8 and fluid channel 5, the chip thief hatch 7 be connected to the sensing chamber 8 by fluid channel 5, the chip body is also
Including electrode 4, the sensing chamber 8 is connected with the electrode 4；The fluid channel 5 includes a mainstream channel 501 and 5 points
Microfluidic channel 502, the end of the mainstream channel 501 distribute 5 described in divide miniflow described in point microfluidic channel 502,5
The mutually independent sensing chamber 8 in body channel 502 and 5, which corresponds, to be connected to；The other end and the chip of the mainstream channel 501
Thief hatch 7 is connected to；The chip body from the bottom up successively include backplane level 1, middle layer 2 and upper cap rock 3, the backplane level 1,
Middle layer 2 matches with upper cap rock 3 and defines closed fluid channel 5 and multiple mutually independent sensing chamber 8；The fluid channel 5
With sensing chamber 8 in the middle layer 2；The upper cap rock 3 is equipped with liquid injection port 701 and 5 gas vent 6,5 gas vents
6 are arranged in the side of the upper cap rock and are arranged at position corresponding with the end of the fluid channel 5, the liquid injection
Mouth 701 is connected with the front end of the fluid channel 5；The backplane level 1 is equipped with electrode 4, the sensing chamber 8 and the electrode 4
It is connected；The flowing of gas vent 6 being provided with conducive to sample, facilitates sample introduction, if inspection cannot be flowed by being not provided with 6 sample of gas vent
It surveys room 8 to be reacted, embeds detection reagent in advance in the sensing chamber 8 of chip；5 fan-shaped distributions of mutually independent sensing chamber 8,
5 points of microfluidic channels 502 are split by the end of the mainstream channel 501 and are connected to 5 mutually independent sensing chamber 8 again；
The backplane level 1, middle layer 2 are bonded by way of 2 Double-faced binded of middle layer integrally with upper cap rock 3；The middle layer 2 is
The material of pressure sensitive adhesive tape, the upper cap rock 3 and/or the backplane level 1 is any one of PMMA, PP, PE, PET, and upper cap rock
3 and backplane level 1 surface have hydrophilic film, so that sample is flowed into mainstream channel 501 quickly through the chip thief hatch 7,
It is diverted to each point of microfluidic channel 502 again；The middle layer 2 with a thickness of 0.1~1.0mm；The surface of the backplane level 1
Be it is flat, the backplane level 1, middle layer 2 matched with upper cap rock 3 define closed fluid channel 5 depth be 0.1~
1.0mm, the width for matching the sensing chamber 8 defined is 1.0~2.0mm；Microfluidic channel 502 is divided to exist described in each
Nozzle is all had with the junction of the sensing chamber 8, and divides microfluidic channel 502 that there is corresponding electrode described in each
4, each electrode 4 includes inputting high lateral electrode and input downside electrode, the electrode 4 with a thickness of 50um；The effect of electrode 4 is
Apply pulse voltage and receives the signal that reaction of blood generates in sensing chamber simultaneously；Electrode end 401 is inserted into detecting instrument, is passed through
The electrochemical signals that detection reaction generates, cooperate mating detecting instrument to obtain testing result；Electrode end 401 is the backplane level
1, relative to the upper cap rock 3 and the exposed part of middle layer 2 after middle layer 2 is bonded integrally with upper cap rock 3, so that electric
Pole end 401 can be easier and easily be inserted into detecting instrument, out testing result；As shown in figures 4-6, the bottom plate
Layer 1, middle layer 2 match with upper cap rock 3 and define closed fluid channel 5, sensing chamber 8 and funnel area 9；The backplane level 1
Lower end side be equipped with a notch 10, the liquid injection port 701, funnel area 9 and notch 10 are separately positioned on cap rock
3, at corresponding position and of different sizes on middle layer 2 and backplane level 1；The chip thief hatch 7 by liquid injection port 701,
Funnel area 9 and notch 10 constitute together and are connected by the fluid channel 5 with the bottom of the sensing chamber 8；The mainstream
Channel 501 is funnel area 9, and the liquid injection port 701 is arc, Chong Die with 9 partial region of funnel area；It is described
It is horn-like that funnel area 9 has opening toe-in to be formed, and the funnel area 9 inwardly disperses 5 points out at its tail end
Microfluidic channel 502 is connected one to one 5 mutually independent sensing chamber 8 by point microfluidic channel 502 described in 5；Wherein
It injects liquid into mouth 701 and is set as arc, it is Chong Die with 9 partial region of funnel area；The funnel area 9 has opening
Toe-in is formed horn-like so that sample gradually flows inward into, and does not stop in opening, sample is avoided to be lost.
Embodiment 3: difference from Example 1 is that the structure of chip thief hatch is different, the backplane level 1, middle layer 2
It is matched with upper cap rock 3 and defines closed fluid channel 5, sensing chamber 8 and funnel area 9；The side of the lower end of the backplane level 1
Equipped with a notch 10, the liquid injection port 701, funnel area 9 and notch 10 are separately positioned on cap rock 3, middle layer 2 and bottom
It is at corresponding position and of different sizes on plate layer 1；The chip thief hatch 7 is by liquid injection port 701, funnel area 9 and lacks
Mouth 10 constitutes together and is connected by the fluid channel 5 with the bottom of the sensing chamber 8；Specifically: the multichannel is quickly examined
Micrometer fluid detection chip, including chip body are provided with chip thief hatch 7, multiple mutually independent on the chip body
Sensing chamber 8 and fluid channel 5, the chip thief hatch 7 are connected to by fluid channel 5 with the sensing chamber 8, and the chip body also wraps
Electrode 4 is included, the sensing chamber 8 is connected with the electrode 4；The fluid channel 5 includes that a mainstream channel 501 and 5 points are micro-
Fluid channel 502, the end of the mainstream channel 501 distribute 5 described in divide microfluid described in point microfluidic channel 502,5
The mutually independent sensing chamber 8 in channel 502 and 5, which corresponds, to be connected to；The other end of the mainstream channel 501 is adopted with the chip
Sample mouth 7 is connected to；The chip body from the bottom up successively include backplane level 1, middle layer 2 and upper cap rock 3, the backplane level 1, in
Interbed 2 matches with upper cap rock 3 and defines closed fluid channel 5 and multiple mutually independent sensing chamber 8；5 He of fluid channel
Sensing chamber 8 is in the middle layer 2；The upper cap rock 3 is equipped with liquid injection port 701 and 5 gas vent 6,5 gas vents 6
The side of the upper cap rock is set and is arranged at position corresponding with the end of the fluid channel 5, the liquid injection
Mouth 701 is connected with the front end of the fluid channel 5；The backplane level 1 is equipped with electrode 4, the sensing chamber 8 and the electrode 4
It is connected；The flowing of gas vent 6 being provided with conducive to sample, facilitates sample introduction, if inspection cannot be flowed by being not provided with 6 sample of gas vent
It surveys room 8 to be reacted, embeds detection reagent in advance in the sensing chamber 8 of chip；5 fan-shaped distributions of mutually independent sensing chamber 8,
5 points of microfluidic channels 502 are split by the end of the mainstream channel 501 and are connected to 5 mutually independent sensing chamber 8 again；
The backplane level 1, middle layer 2 are bonded by way of 2 Double-faced binded of middle layer integrally with upper cap rock 3；The middle layer 2 is
The material of pressure sensitive adhesive tape, the upper cap rock 3 and/or the backplane level 1 is any one of PMMA, PP, PE, PET, and upper cap rock
3 and backplane level 1 surface have hydrophilic film, so that sample is flowed into mainstream channel 501 quickly through the chip thief hatch 7,
It is diverted to each point of microfluidic channel 502 again；The middle layer 2 with a thickness of 0.1~1.0mm；The surface of the backplane level 1
Be it is flat, the backplane level 1, middle layer 2 matched with upper cap rock 3 define closed fluid channel 5 depth be 0.1~
1.0mm, the width for matching the sensing chamber 8 defined is 1.0~2.0mm；Microfluidic channel 502 is divided to exist described in each
Nozzle is all had with the junction of the sensing chamber 8, and divides microfluidic channel 502 that there is corresponding electrode described in each
4, each electrode 4 includes inputting high lateral electrode and input downside electrode, the electrode 4 with a thickness of 50um；The effect of electrode 4 is
Apply pulse voltage and receives the signal that reaction of blood generates in sensing chamber simultaneously；Electrode end 401 is inserted into detecting instrument, is passed through
The electrochemical signals that detection reaction generates, cooperate mating detecting instrument to obtain testing result；Electrode end 401 is the backplane level
1, relative to the upper cap rock 3 and the exposed part of middle layer 2 after middle layer 2 is bonded integrally with upper cap rock 3, so that electric
Pole end 401 can be easier and easily be inserted into detecting instrument, out testing result；As shown in figs. 7-9, the bottom plate
Layer 1, middle layer 2 match with upper cap rock 3 and define closed fluid channel 5, sensing chamber 8 and funnel area 9；The backplane level 1
Lower end side be equipped with a notch 10, the liquid injection port 701, funnel area 9 and notch 10 are separately positioned on cap rock
3, at corresponding position and of different sizes on middle layer 2 and backplane level 1；The chip thief hatch 7 by liquid injection port 701,
Funnel area 9 and notch 10 constitute together and are connected by the fluid channel 5 with the bottom of the sensing chamber 8；It is wherein described
Liquid injection port 701, funnel area 9 and notch 10 are arc and radian is different；The liquid injection port 701, funnel area 9
It is semicircular arc, and the radius of the funnel area 9 is not less than the arc radius of the liquid injection port 701；The funnel
Region 9 disperses 5 points of microfluidic channels 502 and described 5 mutually independent sensing chamber 8 out by curved mainstream channel 501
It is connected to one by one；The area of the notch 10 is less than the area of the funnel area 9.Wherein liquid injection port 701 is semicircular arc,
Area under the same conditions, the sample size of this structure setting injection is most, and the radius of funnel area 9 is not less than the liquid
The arc radius of body inlet 701, so that funnel area 9 can all accommodate the sample liquid injected from liquid injection port, it will not
There is loss；Setting curved channel is unlikely to cause uprushing for 8 atmospheric pressure of sensing chamber so that sample flows slowly into sensing chamber 8.
When specifically used:
To 7 injecting sample of chip thief hatch, sample is led to by flowing to multiple points of microfluids respectively simultaneously by mainstream channel 501
Road 502 enters back into multiple mutually independent sensing chamber 8, and the detection reagent embedded in advance in sample and sensing chamber 8 is reacted,
And the multichannel is quickly detected into microfluid detection chip and is inserted into detecting instrument by electrode end 401, it is reacted by detection
The electrochemical signals of generation cooperate mating detecting instrument to obtain testing result；It may be implemented to detect multiple samples simultaneously in this way, have
There is the effect of multichannel；Improve detection efficiency.
The basic principles and main features and advantage of the utility model have been shown and described above.The technical staff of the industry
It should be appreciated that the present utility model is not limited to the above embodiments, the above embodiments and description only describe this
The principle of utility model, on the premise of not departing from the spirit and scope of the utility model, the utility model also has various change
And improvement, such as the shape and structure of chip thief hatch do the adjustment of some other micro- summaries, these changes and improvements, which are both fallen within, to be wanted
It asks in the scope of the utility model of protection.The utility model requires protection scope is by appended claims and its equivalent circle
It is fixed.
Claims (10)
1. a kind of multichannel quickly detects microfluid detection chip, including chip body, chip is provided on the chip body
Thief hatch, multiple mutually independent sensing chamber and fluid channel, the chip thief hatch are connected to by fluid channel with the sensing chamber,
It is characterized in that, the chip body further includes electrode, the sensing chamber is connected with the electrode；The fluid channel includes one
Mainstream channel and multiple points of microfluidic channels, the end of the mainstream channel distributes multiple point microfluidic channels, more
Divide microfluidic channel to correspond with multiple mutually independent sensing chamber described in a to be connected to；The other end of the mainstream channel and institute
State the connection of chip thief hatch.
2. multichannel according to claim 1 quickly detects microfluid detection chip, which is characterized in that the chip body
It include successively from the bottom up backplane level, middle layer and upper cap rock, the backplane level, middle layer are matched with upper cap rock defines envelope
The fluid channel closed and multiple mutually independent sensing chamber；The fluid channel and sensing chamber are in the middle layer；The upper cap rock is set
Have a liquid injection port and multiple gas vents, the multiple gas vent the side of the upper cap rock is set and setting with it is described micro-
At the corresponding position in the end of runner, the liquid injection port is connected with the front end of the fluid channel；On the backplane level
Equipped with electrode, the sensing chamber is connected with the electrode.
3. multichannel according to claim 2 quickly detects microfluid detection chip, which is characterized in that multiple mutually indepedent
The fan-shaped distribution of sensing chamber, by the end of the mainstream channel be split into multiple points of microfluidic channels be connected to again it is multiple mutually
Independent sensing chamber.
4. multichannel according to claim 3 quickly detects microfluid detection chip, which is characterized in that the chip sampling
Mouthful be made of liquid injection port, the chip thief hatch is connected to the mainstream channel, one end of the mainstream channel with institute
It states and is provided with liquid receiving port at the corresponding position of liquid injection port；The other end of the mainstream channel, which connects, all divides microfluid
Channel.
5. multichannel according to claim 3 quickly detects microfluid detection chip, which is characterized in that the backplane level,
Middle layer matches with upper cap rock and defines closed fluid channel, sensing chamber and funnel area；The one of the lower end of the backplane level
Side is equipped with a notch, and the liquid injection port, funnel area and notch be separately positioned on cap rock, phase on middle layer and backplane level
It is at corresponding position and of different sizes；The chip thief hatch is constituted and is led to together by liquid injection port, funnel area and notch
The fluid channel is crossed to be connected with the bottom of the sensing chamber.
6. multichannel according to claim 5 quickly detects microfluid detection chip, which is characterized in that the liquid injection
Mouth, funnel area and notch are arc and radian is different；The liquid injection port, funnel area are semicircular arc, and institute
The radius for stating funnel area is not less than the arc radius of the liquid injection port；The funnel area passes through curved mainstream channel
Disperse multiple points of microfluidic channels out to be connected to one by one with the multiple mutually independent sensing chamber；The area of the notch is less than institute
State the area of funnel area；
Or the mainstream channel is funnel area, the liquid injection port is arc, with funnel area partial region weight
It is folded；It is horn-like that the funnel area has opening toe-in to be formed, so that sample gradually flows inward into, does not stop in opening
It stays, sample is avoided to be lost；And the funnel area inwardly disperses multiple points of microfluidic channels out at its tail end, passes through multiple institutes
A point microfluidic channel is stated to connect one to one multiple mutually independent sensing chamber.
7. multichannel according to claim 5 quickly detects microfluid detection chip, which is characterized in that the backplane level,
Middle layer is integral by being bonded by way of middle layer Double-faced binded with upper cap rock.
8. quickly detecting microfluid detection chip according to the described in any item multichannels of claim 3-7, which is characterized in that described
Middle layer is pressure sensitive adhesive tape, and the material of the upper cap rock and/or the backplane level is any one of PMMA, PP, PE, PET, and
The surface of the upper cap rock and the backplane level all has hydrophilic film, flows into sample quickly through the chip thief hatch
Mainstream channel, then it is diverted to each point of microfluidic channel.
9. multichannel according to claim 8 quickly detects microfluid detection chip, which is characterized in that the middle layer
With a thickness of 0.1~1.0mm；The surface of the backplane level be it is flat, the backplane level, middle layer are matched with upper cap rock and are defined
The depth of closed fluid channel is 0.1~1.0mm, and the width for matching the sensing chamber defined is 1.0~2.0mm.
10. multichannel according to claim 8 quickly detects microfluid detection chip, which is characterized in that described in each
Point microfluidic channel all has nozzle in the junction with the sensing chamber, and a point microfluidic channel described in each has
Corresponding electrode, each electrode include inputting high lateral electrode and input downside electrode, the electrode with a thickness of 50um.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820904218.3U CN208554242U (en) | 2018-06-12 | 2018-06-12 | A kind of multichannel quickly detects microfluid detection chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820904218.3U CN208554242U (en) | 2018-06-12 | 2018-06-12 | A kind of multichannel quickly detects microfluid detection chip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208554242U true CN208554242U (en) | 2019-03-01 |
Family
ID=65485785
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820904218.3U Active CN208554242U (en) | 2018-06-12 | 2018-06-12 | A kind of multichannel quickly detects microfluid detection chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208554242U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108745429A (en) * | 2018-06-12 | 2018-11-06 | 南京岚煜生物科技有限公司 | A kind of multichannel quickly detects microfluid detection chip |
| CN113278494A (en) * | 2021-05-07 | 2021-08-20 | 深圳市第二人民医院(深圳市转化医学研究院) | Digital PCR microdroplet generation chip |
-
2018
- 2018-06-12 CN CN201820904218.3U patent/CN208554242U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108745429A (en) * | 2018-06-12 | 2018-11-06 | 南京岚煜生物科技有限公司 | A kind of multichannel quickly detects microfluid detection chip |
| CN108745429B (en) * | 2018-06-12 | 2023-11-24 | 南京岚煜生物科技有限公司 | Multichannel rapid detection microfluid detection chip |
| CN113278494A (en) * | 2021-05-07 | 2021-08-20 | 深圳市第二人民医院(深圳市转化医学研究院) | Digital PCR microdroplet generation chip |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108745429A (en) | A kind of multichannel quickly detects microfluid detection chip | |
| CN109682878A (en) | A kind of multi-channel fluid hemostasis examination chip with five-layer structure | |
| EP3932554A1 (en) | Multi-channel microfluidic blood coagulation measurement chip | |
| US11833508B2 (en) | Multi-dimensional double spiral device and methods of use thereof | |
| EP1613433A2 (en) | Fluid partitioning in multiple microchannels | |
| JP5137012B2 (en) | Microchip | |
| CN207786624U (en) | Chemiluminescence testing microfluid control chip and chemiluminescence testing microfluid control chip system | |
| CN107737615A (en) | A kind of microfluidic device for biochemistry detection | |
| CN208554242U (en) | A kind of multichannel quickly detects microfluid detection chip | |
| CN106179545B (en) | Micro-fluidic chip equipment and preparation method thereof for bioanalysis | |
| CN108344876A (en) | Microfluidic assay devices and use its assay method | |
| CN112980677A (en) | Micro-fluidic chip for analyzing and sorting tumor cell migration capacity and preparation process | |
| Saeed et al. | Microfluidic approaches for cancer cell separation | |
| CN209640280U (en) | A kind of multi-channel fluid hemostasis examination chip with five-layer structure | |
| CN109939751B (en) | Microfluidic chip, detection device and detection method for whole blood detection | |
| Kong et al. | Label-free counting of affinity-enriched circulating tumor cells (CTCs) using a thermoplastic micro-C oulter counter (μCC) | |
| CN210230002U (en) | Micro flow channel chip and micro flow channel structure | |
| CN211586658U (en) | Microfluid chip | |
| CN207533259U (en) | ELISA detects micro-fluidic chip and ELISA detection micro-fluidic chip systems | |
| CN111729696A (en) | Microfluid detection chip adopting immune electrode | |
| CN205643133U (en) | Optical profile type biosensor and little fluid means's structure of integrating | |
| CN104062445A (en) | Microfluid chip detection technique based on photonic crystal encoded microsphere | |
| CN210115073U (en) | Micro flow channel chip and micro flow channel structure | |
| TWM581591U (en) | Microchannel chip having slack flow block with small aperture, and microchannel structure | |
| CN215655207U (en) | Micro-fluidic chip |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |