CN215655207U

CN215655207U - Micro-fluidic chip

Info

Publication number: CN215655207U
Application number: CN202121714823.2U
Authority: CN
Inventors: 许行尚; 杰弗瑞·陈
Original assignee: Nanjing Lanyu Biological Technology Co Ltd
Current assignee: Nanjing Lanyu Biological Technology Co Ltd
Priority date: 2021-07-27
Filing date: 2021-07-27
Publication date: 2022-01-28
Anticipated expiration: 2031-07-27

Abstract

The utility model discloses a micro-fluidic chip which comprises a chip body, wherein the chip body at least comprises a lower chip, a middle chip and an upper chip from bottom to top in sequence; the chip body comprises a sample injection cavity, a plurality of cavities and a micro-channel, wherein the sample injection cavity is communicated with the plurality of cavities through the micro-channel; the plurality of chambers at least comprise two reaction chambers, the micro-channel is provided with a main input point, and the flow paths of the sample fluid to be detected from the main input point to each reaction chamber along the micro-channel are equal. The micro-fluidic chip can realize the simultaneous measurement of a plurality of coagulation indexes of a sample fluid to be detected, and has a multi-channel effect; the design is reasonable, the structure is simple and compact, and the production cost is reduced; the chip has simple structure and convenient operation, improves the detection efficiency and greatly reduces the consumption of resources; and the rapid detection is realized, and the cost is reduced.

Description

Micro-fluidic chip

Technical Field

The utility model belongs to the technical field of medical equipment, and particularly relates to a micro-fluidic chip.

Background

Microfluidics is a technology applied across various disciplines including engineering, physics, chemistry, microtechnology, and biotechnology. Microfluidics involves the study of minute quantities of fluids and how to manipulate, control and use such small quantities of fluids in various microfluidic systems and devices, such as microfluidic chips. For example: microfluidic biochips (known as "lab-on-a-chip") are used in the field of molecular biology to integrate assay operations for purposes such as analyzing enzymes and DNA, detecting biochemical toxins and pathogens, diagnosing diseases, and the like.

In order to realize rapid and efficient blood coagulation detection and analysis, a microfluidic blood coagulation detection chip is designed and manufactured on the basis of the traditional blood coagulation detection method, a chip motherboard is processed by utilizing the traditional photoetching and wet etching method, and then the required chip is obtained by PDMS copying and molding; when the coagulation is detected, a trace amount of blood sample and coagulant are respectively added into the sample holes at the two sides. By utilizing the laminar flow diffusion characteristic of the reagents in the micro-channel, the two reagents are gradually solidified after being mutually diffused and mixed in the main channel. The distance from the start of mixing to the freezing point represents the coagulation characteristics of the blood sample. Microfluidic chips (microfluidic chips) are a hot spot area for the development of current micro Total Analysis Systems (minidesigned Total Analysis Systems). The micro-fluidic chip analysis takes a chip as an operation platform, simultaneously takes analytical chemistry as a basis, takes a micro-electromechanical processing technology as a support, takes a micro-pipeline network as a structural characteristic, takes life science as a main application object at present, and is the key point of the development of the field of the current micro total analysis system. Its goal is to integrate the functions of the entire laboratory, including sampling, dilution, reagent addition, reaction, separation, detection, etc., on a microchip. The micro-fluidic chip is a main platform for realizing the micro-fluidic technology. The device is characterized in that the effective structure (channels, reaction chambers and other functional components) for containing the fluid is at least in one latitude in micron scale. Due to the micro-scale structure, the fluid exhibits and develops specific properties therein that differ from those of the macro-scale. Thus developing unique assay-generated properties. The characteristics and development advantages of the micro-fluidic chip are as follows: the micro-fluidic chip has the characteristics of controllable liquid flow, extremely less consumption of samples and reagents, ten-fold or hundred-fold improvement of analysis speed and the like, can simultaneously analyze hundreds of samples in a few minutes or even shorter time, and can realize the whole processes of pretreatment and analysis of the samples on line. The application of the micro total analysis system aims to realize the ultimate goal of the micro total analysis system, namely a lab-on-a-chip, and the key application field of the current work development is the field of life science. Compared with the traditional blood coagulation detection method by using a glass test tube, the blood coagulation detection method based on the microfluidic chip is simple and efficient, has high detection speed, less reagent consumption, low cost and good repeatability of detection results, and can be used for simultaneously detecting multiple reagents. Moreover, the detection device based on the blood coagulation chip can be miniaturized and is convenient to carry, thereby being beneficial to clinical popularization. However, when the current microfluidic chip detects a plurality of detection indexes at the same time, the flow of each index is difficult to control accurately, and synchronous reaction is difficult to realize.

SUMMERY OF THE UTILITY MODEL

The utility model aims to solve the technical problem of providing a microfluidic chip which can accurately control the flow of a sample fluid to be detected in a plurality of reaction cavities, realize independent and synchronous reaction of the reaction cavities, realize simultaneous measurement of a plurality of coagulation indexes of the sample fluid to be detected, has a multi-channel effect, has a simple chip structure, is convenient to operate, improves the detection efficiency and greatly reduces the consumption of resources; and the rapid detection is realized, and the cost is reduced.

In order to solve the technical problems, the utility model adopts the technical scheme that the micro-fluidic chip comprises a chip body, wherein the chip body at least comprises a lower chip, a middle chip and an upper chip from bottom to top in sequence; the chip body comprises a sample injection cavity, a plurality of cavities and a micro-channel, wherein the sample injection cavity is communicated with the plurality of cavities through the micro-channel; the plurality of chambers at least comprise two reaction chambers, the micro-channel is provided with a main input point, and the flow paths of the sample fluid to be detected from the main input point to each reaction chamber along the micro-channel are equal.

By adopting the technical scheme, the microfluid detection chip has the characteristics of high precision, high speed and low detection cost, and is suitable for detection in a precise medical link. According to the design, the magnetic beads and the detection reagent mixture are embedded in each reaction cavity in advance, so that multiple coagulation indexes of one sample fluid to be detected can be measured simultaneously, and the multichannel effect is achieved; the design is reasonable, the structure is simple and compact, and the production cost is reduced; the chip has simple structure and convenient operation, improves the detection efficiency and greatly reduces the consumption of resources; and the rapid detection is realized, and the cost is reduced.

As the preferred technical scheme of the utility model, the middle-layer chip and the lower-layer chip are matched to define a micro-channel and a plurality of chambers; the reaction cavities which are independent from each other are respectively arranged on two sides of the micro flow channel. The reaction chambers are respectively arranged at two sides of the micro-channel, namely the reaction chambers are designed at two sides of the chip body, and the arrangement ensures that the magnetic field loaded on the side surface of the micro-fluidic chip is more accurate when the micro-fluidic chip is used specifically.

As a preferred technical scheme, the sample injection cavity comprises a first sample injection cavity, a second sample injection cavity and a third sample injection cavity, the first sample injection cavity is used for adding a sample to be detected, the second sample injection cavity is used for adding a dissociation agent, the third sample injection cavity is used for adding a cleaning solution, and the first sample injection cavity, the second sample injection cavity and the third sample injection cavity are communicated with each other through the micro-channel.

As a preferable technical scheme, the cavity comprises a waste liquid cavity, the micro flow channel comprises a reaction input flow channel and a reaction output flow channel, the reaction input flow channel is arranged at one end of the reaction cavity, the reaction output flow channel is arranged at the other end of the reaction cavity, and the reaction output flow channel is communicated with the waste liquid cavity.

As a preferred technical solution of the present invention, the reaction chamber includes a reaction chamber through hole disposed in the middle chip and a reaction chamber disposed in the lower chip, and the position of the reaction chamber through hole in the middle chip corresponds to the position of the reaction chamber in the lower chip; the waste liquid cavity is composed of a lower waste liquid cavity arranged on the front surface of the lower chip, a middle waste liquid cavity through hole arranged on the middle chip in a penetrating manner and an upper waste liquid cavity arranged on the back surface of the upper chip.

As a preferable technical proposal of the utility model, the micro flow channel also comprises a first input flow channel, a second input flow channel, a third input flow channel, a fourth input flow channel and a fifth input flow channel, one end of the first sample introduction cavity is connected with the first input flow channel, one end of the second sample introduction cavity is connected with the second input flow channel, one end of the third sample introduction cavity is connected with the third input flow channel, the other ends of the first input flow channel, the second input flow channel and the third input flow channel are communicated and converged at a first input point, the first input flow channel, the second input flow channel, the third input flow channel and the fifth input flow channel are all arranged on the lower layer chip, the fourth input flow channel is arranged on the middle-layer chip, the sample fluid to be detected sequentially passes through the fourth input flow channel and the fifth input flow channel from the first input point and then flows to the main input point, and the main input point is connected with the reaction input flow channel. Specifically, the number of the reaction chambers is 4, and the reaction input flow channels include a first reaction input flow channel, a first reaction input flow channel a, a first reaction input flow channel b, a second reaction input flow channel a and a second reaction input flow channel b, the sample fluid to be detected sequentially flows from the main input point to the first reaction chamber through the first reaction input flow channel and the first reaction input flow channel a, the sample fluid to be detected sequentially flows from the main input point to the second reaction chamber through the first reaction input flow channel and the first reaction input flow channel b, the sample fluid to be detected sequentially flows from the main input point to the third reaction chamber through the second reaction input flow channel and the second reaction input flow channel a, and the sample fluid to be detected sequentially flows from the main input point to the second reaction input flow channel, The second reaction input flow channel b flows to the fourth reaction cavity; the flows of the sample fluid to be detected from the main input point to the first reaction cavity, the second reaction cavity, the third reaction cavity and the fourth reaction cavity are equal, so that the synchronous reaction of the sample fluid to be detected in the first reaction cavity, the second reaction cavity, the third reaction cavity and the fourth reaction cavity is realized. By designing the micro-channel with a specific structural form and guiding the flow of the blood sample, the samples can be injected into the reaction cavities simultaneously through one sample injection cavity without polluting the samples, and the sample injection is easy; the chip has simple structure and convenient operation, improves the detection efficiency and greatly reduces the consumption of resources; and the rapid detection is realized, and the cost is reduced.

As a preferred technical scheme of the present invention, each reaction chamber is provided with a rubber conductive valve, the reaction output channel comprises a first reaction output channel, a second reaction output channel and a waste liquid output channel, the reaction chamber is provided with two output paths, and the first output path is that the reaction chamber is communicated with the waste liquid output channel through the first reaction output channel and the rubber conductive valve; the second output path is that the reaction chamber is communicated with the waste liquid output channel through the second reaction output channel and the switch valve; the waste liquid output flow channel is communicated with the waste liquid cavity. The first reaction output flow channel comprises a first reaction cavity output flow channel, a first connecting flow channel a and a first connecting flow channel b, and the reaction cavity sequentially passes through the first reaction cavity output flow channel, the first connecting flow channel a, the rubber conductive valve and the first connecting flow channel b and then is communicated with the waste liquid output flow channel; the switch valve comprises a switch flow passage a, a switch flow passage b and a switch hole, wherein the switch flow passage a and the switch flow passage b are arranged on the middle-layer chip, and the switch hole is arranged on the lower-layer chip; the first reaction cavity output flow channel is arranged on the reaction cavity through hole of the middle chip, the first connecting flow channel a and the first connecting flow channel b are arranged on the lower chip, the second reaction output flow channel is arranged on the reaction cavity of the lower chip, and the first connecting flow channel a and the first connecting flow channel b are arranged on the middle chip. The method specifically comprises the following steps: after the sample fluid to be detected is added into the first sample introduction cavity, the sample fluid to be detected flows through the fourth input flow channel from the first input flow channel and then flows through the fifth input flow channel to enter the main flow point, the sample fluid to be detected simultaneously flows to a plurality of mutually independent reaction cavities through the main flow point, a mixture of magnetic beads and a detection reagent is embedded in the reaction cavities in advance, after the reaction cavities are filled with the sample fluid to be detected, the rubber conductive valves matched with the reaction cavities are closed, the sample fluid to be detected reacts in the reaction cavities, after the reaction is finished, open with the supporting ooff valve of each reaction chamber, liquid gets into the waste liquid chamber through ooff valve, waste liquid output runner, and rethread advances appearance chamber three and adds the washing liquid and wash miniflow channel and reaction chamber, adds the dissociation agent from advancing appearance chamber two again after the washing, and the dissociation agent gets into and reacts after each reaction chamber, discharges to the waste liquid chamber after the reaction.

As a preferred technical solution of the present invention, the rubber conductive valve includes an upper layer conductive rubber valve structure disposed on the upper layer chip and a middle layer conductive rubber valve structure disposed at a position corresponding to the middle layer chip.

As a preferred technical scheme of the utility model, the sample injection cavity comprises a sample injection port penetrating through the upper layer chip, a middle layer liquid injection through hole penetrating through the corresponding position of the middle layer chip and a sample injection cavity body arranged on the front surface of the lower layer chip, and a middle layer exhaust hole penetrates through the upper part of the middle layer liquid injection through hole. The upper chip is provided with the plurality of exhaust holes in a penetrating manner, so that the flow resistance of the fluid to be detected is reduced, the flow is quicker, and the reaction cavity is quickly filled; the exhaust hole is arranged to facilitate the flow of the sample, the sample introduction is convenient, and if the exhaust hole is not arranged, the sample cannot flow into the reaction cavity to react.

As the preferred technical scheme of the utility model, the middle layer liquid adding through hole is fan-shaped, and a middle layer exhaust hole is arranged above the middle layer liquid adding through hole in a penetrating manner; the appearance cavity of advancing is fan-shaped for the banana, including straining appearance pond and water conservancy diversion district, the liquid outlet setting in the narrow limit lateral wall in straining appearance pond, the water conservancy diversion district sets up strain the bottom in appearance pond, the top of advance appearance cavity is equipped with a plurality of bleeder vent, strain the position department of the bottom of the pool in appearance pond near wide lateral wall, correspond each the bleeder vent all is provided with a guide slot, guide slot with be provided with gaseous gathering area between the water conservancy diversion district.

As a preferred technical solution of the present invention, the flow guide area is at least divided into two flow guide areas at the bottom of the sample filtering pool according to the fluid flow direction, the two flow guide areas are capable of communicating with each other, each flow guide area is provided with a plurality of flow guide strips distributed in a gathering manner, and the distribution density of the flow guide strips in the flow guide area at the front end of the fluid flow direction is less than the distribution density of the flow guide strips in the flow guide area at the rear end of the fluid flow direction.

As the preferable technical scheme of the utility model, the bottom of the sample filtering pool is sequentially provided with a first flow guide area and a second flow guide area according to the flow direction of fluid; the first diversion area comprises a plurality of first-level diversion bodies and a plurality of secondary diversion bodies, the first-level diversion bodies and the plurality of secondary diversion bodies are both ridge-shaped bulges, the cross section size of each first-level diversion body is larger than that of each secondary diversion body, the length of each first-level diversion body is consistent with that of each secondary diversion body, and the plurality of secondary diversion bodies are uniformly distributed between every two adjacent first-level diversion bodies.

As a preferred technical solution of the present invention, the second diversion area is disposed at a position corresponding to the first-stage diversion body, and a diversion strip is disposed along a length extending direction of the first-stage diversion body, the diversion strip of the second diversion area is a ridge, and a cross-sectional dimension of the ridge of the second diversion area is not greater than a cross-sectional dimension of the first-stage diversion body.

As a preferred technical scheme of the utility model, the number of the air guide grooves is 3, one end of each air guide groove, which is close to the side wall of the wide side of the sample filtering pool, is communicated with the air holes in a one-to-one correspondence manner, and a notch at the other end of each air guide groove is arranged and communicated with the gas polymer area.

As a preferred technical solution of the present invention, the number of the first flow guiding bodies in the first flow guiding zone is 3, and correspondingly, the number of the flow guiding bodies in the second flow guiding zone is 3, and the cross-sectional size of the ridge protrusion of the second flow guiding zone is the same as the cross-sectional size of the first flow guiding body.

The utility model is further improved in that the upper chip, the middle chip and the lower chip are bonded into a whole in a double-sided gluing mode through the middle chip.

As a preferred technical solution of the present invention, the middle chip is a pressure sensitive adhesive tape, the material of the upper chip and/or the lower chip is any one of PMMA, PP, PE, and PET, and the surfaces of the upper chip and the lower chip are both provided with hydrophilic films, so that the sample rapidly flows through the sampling port into the main flow channel and then flows into each sub-microfluidic channel. By adopting the technical scheme, the materials are easy to obtain, and the thickness of the pressure-sensitive adhesive tape can be precisely controlled by the manufacturing process of the pressure-sensitive adhesive tape, so that by adopting the technical scheme, the depth and the size of the micro-channel can be precisely controlled, and meanwhile, the depth of the reaction cavity is convenient to control, so that the thickness deviation of each reaction cavity of the micro-fluid chip is small, the consistency is high, and the detection accuracy is improved.

As the preferred technical scheme of the utility model, the thickness of the middle layer chip is 0.1-1.0 mm; the surface of the lower chip is flat, the depth of a closed micro-channel defined by the lower chip, the middle chip and the upper chip in a matched manner is 0.1-1.0 mm, and the width of the reaction cavity defined by the lower chip, the middle chip and the upper chip in a matched manner is 1.0-2.0 mm.

Compared with the prior art, the micro-fluidic chip has the advantages of high detection speed, miniaturization, low cost, good repeatability, synchronous detection and the like. The micro-fluidic chip realizes that the flow paths of the sample fluid to be detected from the main input point to each reaction cavity along the micro-channel are equal by arranging the main input point on the micro-channel, so that the sample fluid to be detected in the reaction cavities independently and synchronously reacts. According to the design, the magnetic beads and the detection reagent mixture are embedded in each reaction cavity in advance, so that multiple coagulation indexes of one sample fluid to be detected can be measured simultaneously, and the multichannel effect is achieved; the design is reasonable, the structure is simple and compact, and the production cost is reduced; the chip has simple structure and convenient operation, improves the detection efficiency and greatly reduces the consumption of resources; and the rapid detection is realized, and the cost is reduced.

Drawings

The following further detailed description of embodiments of the utility model is made with reference to the accompanying drawings:

FIG. 1 is a schematic diagram of the front structure of a microfluidic chip according to the present invention;

FIG. 2 is a schematic diagram of the back structure of the microfluidic chip of the present invention;

FIG. 3 is an exploded schematic view of the front side of a microfluidic chip according to the present invention;

FIG. 4 is an exploded perspective view of the front face of the microfluidic chip of the present invention;

FIG. 5 is an exploded schematic plan view of the front side of a microfluidic chip according to the present invention;

FIG. 6 is an exploded schematic plan view of the back side of a microfluidic chip according to the present invention;

wherein: 1-lower chip; 2-middle layer chip; 3-upper chip; 4-sample introduction cavity; 401-sample introduction cavity one; 4011-sample introduction cavity; 4012-middle layer liquid adding through hole; 4013-an upper sample entry port; 4014 — upper vent; 4015-middle layer exhaust vent; 402-sample introduction cavity II; 403-sample introduction chamber III; 5-a chamber; 6-micro flow channel; 601-a primary input point; 602-a reaction input flow channel; 6021-a first reaction input flow channel; 6022-first reaction input flow channel a; 6023-first reaction input flow channel b; 6024-second reaction input flow channel; 6025-second reaction input flow channel a; 6026-second reaction input flow channel b; 603-a reaction output flow channel; 6031-first reaction output flow channel; 60311-first reaction chamber output flow channel; 60312-first connecting flow path a; 60313-first connecting flow path b; 6032-second reaction output flow channel; 6033-waste liquid output flow channel; 604-a first input flow channel; 605-a second input flow channel; 606-a third input flow channel; 607-a fourth input channel; 608-a fifth input flow channel; 609 — a first input point; 7-waste liquid chamber; 701-lower waste liquid chamber; 702-a middle layer waste liquid cavity through hole; 703-upper waste liquid chamber; 704-waste liquid connection switch valve; 8-a reaction chamber; 801-a reaction chamber; 802-reaction chamber through hole; 8-1-a first reaction chamber; 8-2-a second reaction chamber; 8-3-a third reaction chamber; 8-4-a fourth reaction chamber; 9-a rubber conductive valve; 901-upper conductive rubber valve structure; 902-middle layer conductive rubber valve structure; 10-a switch valve; 1001-switching flow channel a; 1002-switching flow channel b; 1003-switch hole.

Detailed Description

Example (b): as shown in fig. 1 to 6, the microfluidic chip comprises a chip body, wherein the chip body at least comprises a lower chip 1, a middle chip 2 and an upper chip 3 from bottom to top in sequence; the chip body comprises a sample injection cavity 4, a plurality of cavities 5 and a micro-channel 6, wherein the sample injection cavity 4 is communicated with the plurality of cavities 5 through the micro-channel 6; the plurality of chambers 5 comprise 4 reaction chambers 8, the microchannel is provided with a main input point 601, and the flow paths of the sample fluid to be detected from the main input point 601 to each reaction chamber along the microchannel are equal; the middle chip 2 and the lower chip 1 are matched to define a micro-channel and a plurality of chambers 5; the 4 mutually independent reaction cavities are respectively arranged at two sides of the micro-channel 6; 4 reaction cavities are respectively arranged at two sides of a micro-channel 6, namely the reaction cavities are arranged at two sides of a chip body, and the arrangement ensures that a magnetic field loaded on the micro-fluidic chip is more accurate when the micro-fluidic chip is used specifically; the sample injection cavity 4 comprises a first sample injection cavity 401, a second sample injection cavity 402 and a third sample injection cavity 403, wherein the first sample injection cavity 401 is used for adding a sample to be detected, the second sample injection cavity 402 is used for adding a dissociation agent, and the second sample injection cavity 402 comprises a dissociation agent adding through hole arranged on the upper chip 3, a dissociation agent adding hole arranged on the middle chip 2 and a dissociation agent cavity arranged on the lower chip 1; the sample feeding cavity III 403 is used for adding cleaning fluid, and the sample feeding cavity I401, the sample feeding cavity II 402 and the sample feeding cavity III 403 are communicated with the micro flow channel 6; the cavity 5 comprises a waste liquid cavity 7, and the waste liquid cavity 7 is composed of a lower waste liquid cavity 701 arranged on the front surface of the lower chip 1, a middle waste liquid cavity through hole 702 penetrating the middle chip 2 and an upper waste liquid cavity 703 arranged on the back surface of the upper chip 3; the positions of the lower waste liquid cavity 701, the middle waste liquid cavity through hole 702 and the upper waste liquid cavity 703 correspond to each other; the micro flow channel 6 comprises a reaction input flow channel 602 and a reaction output flow channel 603, the reaction input flow channel 602 is arranged at one end of the reaction cavity 8, the reaction output flow channel 603 is arranged at the other end of the reaction cavity 8, and the reaction output flow channel 603 is communicated with the waste liquid cavity 7; the reaction cavity 8 comprises a reaction cavity through hole 802 arranged in the middle chip 2 and a reaction cavity 801 arranged in the lower chip 1, and the position of the reaction cavity through hole 802 in the middle chip 2 corresponds to the position of the reaction cavity 801 in the lower chip 1; the microchannel 6 further includes a first input channel 604, a second input channel 605, a third input channel 606, a fourth input channel 607 and a fifth input channel 608, one end of the first sample introduction cavity 401 is connected to the first input channel 604, one end of the second sample introduction cavity 402 is connected to the second input channel 605, one end of the third sample introduction cavity 403 is connected to the third input channel 606, the other ends of the first input channel 604, the second input channel 605 and the third input channel 606 are communicated and converged at a first input point 609, the first input channel 604, the second input channel 605, the third input channel 606 and the fifth input channel 608 are all arranged on the lower chip 1, the fourth input channel 607 is arranged on the middle chip 2, a sample fluid to be detected sequentially passes through the fourth input channel 607 and the fifth input channel 608 from the first input point 609 and then flows to the main input point 601, the main input point 601 is connected to the reaction input runner 602; the tail end of the fifth input flow channel 608 is communicated with the waste liquid cavity 7 through a waste liquid connection switch valve 704; specifically, the number of the reaction chambers 8 is 4, and the reaction input flow channel 602 includes a first reaction input flow channel 6021, a first reaction input flow channel a6022, a first reaction input flow channel b6023, a second reaction input flow channel 6024, a second reaction input flow channel a6025, and a second reaction input flow channel b6026, the sample fluid to be detected flows from the main input point 601 to the first reaction chamber 8-1 through the first reaction input flow channel 6021 and the first reaction input flow channel a6022 in sequence, the sample fluid to be detected flows from the main input point 601 to the second reaction chamber 8-2 through the first reaction input flow channel 6021 and the first reaction input flow channel b6023 in sequence, and the sample fluid to be detected flows from the main input point 601 to the second reaction input flow channel 6024 and the second reaction input flow channel 6024 in sequence, The second reaction input flow channel a6025 flows to the third reaction chamber 8-3, and the sample fluid to be detected flows from the main input point 601 to the fourth reaction chamber 8-4 through the second reaction input flow channel 6024 and the second reaction input flow channel b6026 in sequence; the flows of the sample fluid to be detected from the main input point 601 to the first reaction cavity 8-1, the second reaction cavity 8-2, the third reaction cavity 8-3 and the fourth reaction cavity 8-4 are equal, so that the synchronous reaction of the sample fluid to be detected in the first reaction cavity 8-1, the second reaction cavity 8-2, the third reaction cavity 8-3 and the fourth reaction cavity 8-4 is realized; by designing the micro-channel with a specific structural form and guiding the flow of the blood sample, the samples can be injected into the reaction cavities simultaneously through one sample injection cavity without polluting the samples, and the sample injection is easy; the chip has simple structure and convenient operation, improves the detection efficiency and greatly reduces the consumption of resources; the rapid detection is realized, and the cost is reduced; each reaction cavity 8 is provided with a rubber conductive valve 9, the reaction output channel 603 comprises a first reaction output channel 6031, a second reaction output channel 6032 and a waste liquid output channel 6033, the reaction cavity 8 is provided with two output paths, and the first output path is that the reaction cavity 8 is communicated with the waste liquid output channel 6033 through the first reaction output channel 6031 and the rubber conductive valve 9; the second output path is that the reaction chamber 8 is communicated with the waste liquid output channel 6033 through the second reaction output channel 6032 and the switch valve 10; the waste liquid output channel 6033 is communicated with the waste liquid cavity 7; the first reaction output channel 6031 comprises a first reaction cavity output channel 60311, a first connection channel a60312 and a first connection channel b60313, and the reaction cavity 8 sequentially passes through the first reaction cavity output channel 60311, the first connection channel a60312, the rubber conductive valve 9 and the first connection channel b60313 and then is communicated with the waste liquid output channel 6033; the switch valve 10 comprises a switch flow channel a1001, a switch flow channel b1002 and a switch hole 1003, wherein the switch flow channel a1001 and the switch flow channel b1002 are arranged on the middle chip 2, and the switch hole 1003 is arranged on the lower chip 1; the structure of the waste liquid switch connecting valve 704 is the same as that of the switch valve 10; the first reaction cavity output flow passage 60311 is arranged on the reaction cavity through hole 802 of the middle chip 2, the first connection flow passage a60312 and the first connection flow passage b60313 are both arranged on the lower chip 1, the second reaction output flow passage 6032 is arranged on the reaction cavity 801 of the lower chip 1, and the first connection flow passage a60312 and the first connection flow passage b60313 are both arranged on the lower chip 1; the rubber conductive valve 9 comprises an upper layer conductive rubber valve structure 901 arranged on the upper layer chip 3 and a middle layer conductive rubber valve structure 902 arranged at a position corresponding to the middle layer chip 2; the first sample injection cavity 401 comprises a sample injection port 4013 which penetrates through the upper chip, a middle layer liquid adding through hole 4012 which penetrates through the middle layer chip 2 and is arranged at the corresponding position, and a sample injection cavity 4011 which is arranged on the front surface of the lower chip 1, wherein an upper layer vent 4014 penetrates through the upper part of the upper sample injection port 4013, and a plurality of vent holes 4014 penetrate through the upper chip 3, so that the flow resistance of the fluid to be detected is reduced, the flow is quicker, and the reaction cavity 8 is quickly filled; the arrangement of the vent hole is beneficial to the flow of the sample and is convenient for sample introduction, and if the vent hole is not arranged, the sample cannot flow into the reaction cavity 8 for reaction; the middle layer liquid adding through hole 4012 is fan-shaped, and a middle layer exhaust hole 4015 penetrates above the middle layer liquid adding through hole 4012.

In addition, the sample introduction cavity 4011 is fan-shaped, and comprises a sample filtering pool and a flow guide area, wherein a liquid outlet of the sample filtering pool is arranged on the narrow side wall, the flow guide area is arranged at the bottom of the sample filtering pool, a plurality of air holes are formed in the top of the sample introduction cavity, an air guide groove is formed in the position, close to the wide side wall, of the bottom of the sample filtering pool and corresponds to each air hole, and a gas gathering area is arranged between each air guide groove and the flow guide area; the flow guide area is at least divided into two flow guide areas in which fluids can be mutually communicated at the bottom of the sample filtering pool according to the flow direction of the fluids, each flow guide area is provided with a plurality of flow guide strips which are distributed in a gathering shape, and the distribution density of the flow guide strips in the flow guide area at the front end of the flow direction of the fluids is smaller than that of the flow guide strips in the flow guide area at the rear end of the flow direction of the fluids; the bottom of the sample filtering pool is sequentially provided with a first flow guide area and a second flow guide area according to the flow direction of the fluid; the first diversion area comprises a plurality of first-stage diversion bodies and a plurality of second-stage diversion bodies, the first-stage diversion bodies and the second-stage diversion bodies are all rib protrusions, the size of the cross section of each first-stage diversion body is larger than that of the cross section of each second-stage diversion body, the length of each first-stage diversion body is consistent with that of each second-stage diversion body, and the plurality of second-stage diversion bodies are uniformly distributed between every two adjacent first-stage diversion bodies; the second diversion area is arranged at a position corresponding to the first-stage diversion body and is provided with a diversion strip along the length extension direction of the first-stage diversion body, the diversion strip of the second diversion area is a rib protrusion, and the cross section size of the rib protrusion of the second diversion area is not larger than that of the first-stage diversion body; the number of the gas guide grooves is 3, one end of each gas guide groove, which is close to the side wall of the wide edge of the sample filtering pool, is communicated with the air holes in a one-to-one correspondence manner, and a notch at the other end of each gas guide groove is arranged and communicated with the gas polymer area; the number of the first flow guiding bodies in the first flow guiding area is 3, correspondingly, the number of the flow guiding bodies in the second flow guiding area is 3, and the size of the cross section of the ridge of the second flow guiding area is the same as that of the cross section of the first flow guiding body; the upper layer chip, the middle layer chip and the lower layer chip are bonded into a whole in a double-sided gluing mode through the middle layer chip; the middle chip is a pressure-sensitive adhesive tape, the upper chip and/or the lower chip is made of any one of PMMA, PP, PE and PET, and the surfaces of the upper chip and the lower chip are respectively provided with a hydrophilic film, so that a sample can rapidly flow through the sampling port to enter the main flow channel and then flow to each sub-microfluidic channel. By adopting the technical scheme, materials are easy to obtain, and the thickness of the pressure-sensitive adhesive tape can be precisely controlled by the manufacturing process of the pressure-sensitive adhesive tape, so that the depth and the size of the micro-channel can be precisely controlled, the depth of the reaction cavity can be conveniently controlled, the thickness deviation of each reaction cavity of the micro-fluid chip is small, the consistency is high, and the detection accuracy is improved; the thickness of the middle layer chip is 0.1-1.0 mm; the surface of the lower chip is flat, the depth of a closed micro-channel defined by the lower chip, the middle chip and the upper chip in a matched manner is 0.1-1.0 mm, and the width of the reaction cavity defined by the lower chip, the middle chip and the upper chip in a matched manner is 1.0-2.0 mm.

The method specifically comprises the following steps: after the sample fluid to be detected is added into the first sample injection cavity 401, the sample fluid to be detected flows through the fourth input flow channel 607 from the first input flow channel 604 and then flows through the fifth input flow channel 608 to enter the main flow point 601, the sample fluid to be detected simultaneously flows through the main flow point 601 to the plurality of mutually independent reaction cavities 8, a mixture of magnetic beads and a detection reagent is embedded in each reaction cavity 8 in advance, after the sample fluid to be detected is filled in each reaction cavity 8, the rubber conductive valve 9 matched with each reaction cavity 8 is closed, the sample fluid to be detected reacts in the reaction cavities 8, after the reaction is finished, the switch valve 10 matched with each reaction cavity 8 is opened, liquid enters the waste liquid cavity 7 through the switch valve 10 and the waste liquid output flow channel 6033, cleaning liquid is added into the third sample injection cavity 403 and flows through the third input flow channel 606 to clean the micro flow channel and the reaction cavities, after the cleaning is finished, a dissociation agent is added into the second sample injection cavity 402 and flows through the second input flow channel 605 to enable the dissociation agent to enter each reaction cavity 8 and then flows through the second input flow channel 605 And (4) reacting, and discharging to a waste liquid cavity 7 after the reaction is finished.

The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are only illustrative of the principles of the present invention, but that various changes and modifications, such as the number of layers of chips, the shape and number of reaction chambers, etc., may be made without departing from the spirit and scope of the utility model, and these changes and modifications are within the scope of the utility model as claimed. The scope of the utility model is defined by the appended claims and equivalents thereof.

Claims

1. A micro-fluidic chip comprises a chip body, wherein the chip body at least comprises a lower chip, a middle chip and an upper chip from bottom to top in sequence; the chip body comprises a sample injection cavity, a plurality of cavities and a micro-channel, wherein the sample injection cavity is communicated with the plurality of cavities through the micro-channel; the device is characterized in that the plurality of chambers at least comprise two reaction chambers, the micro-channel is provided with a main input point, and the flow paths of the sample fluid to be detected from the main input point to each reaction chamber along the micro-channel are equal.

2. The microfluidic chip according to claim 1, wherein the middle chip and the lower chip cooperate to define a microchannel and a plurality of chambers; the reaction cavities which are independent from each other are respectively arranged on two sides of the micro flow channel.

3. The microfluidic chip according to claim 1, wherein the sample injection cavity comprises a first sample injection cavity, a second sample injection cavity and a third sample injection cavity, the first sample injection cavity is used for adding a sample to be tested, the second sample injection cavity is used for adding a dissociation agent, the third sample injection cavity is used for adding a cleaning solution, and the first sample injection cavity, the second sample injection cavity and the third sample injection cavity are all communicated with the micro flow channel.

4. The microfluidic chip according to claim 3, wherein the chamber comprises a waste liquid chamber, the microchannel comprises a reaction input channel and a reaction output channel, the reaction input channel is disposed at one end of the reaction chamber, the reaction output channel is disposed at the other end of the reaction chamber, and the reaction output channel is communicated with the waste liquid chamber.

5. The microfluidic chip according to claim 4, wherein the reaction chamber comprises a reaction chamber through hole disposed on the middle chip and a reaction chamber disposed on the lower chip, and the reaction chamber through hole is disposed at the middle chip corresponding to the reaction chamber at the lower chip; the waste liquid cavity is composed of a lower waste liquid cavity arranged on the front surface of the lower chip, a middle waste liquid cavity through hole arranged on the middle chip in a penetrating manner and an upper waste liquid cavity arranged on the back surface of the upper chip.

6. The microfluidic chip according to claim 5, wherein the micro flow channel further comprises a first input flow channel, a second input flow channel, a third input flow channel, a fourth input flow channel and a fifth input flow channel, one end of the first sample chamber is connected to the first input flow channel, one end of the second sample chamber is connected to the second input flow channel, one end of the third sample chamber is connected to the third input flow channel, the other ends of the first input flow channel, the second input flow channel and the third input flow channel are connected to each other and collected at a first input point, the first input flow channel, the second input flow channel, the third input flow channel and the fifth input flow channel are all arranged on the lower chip, the fourth input flow channel is arranged on the middle chip, and the sample fluid to be detected sequentially passes through the fourth input flow channel and the fifth input flow channel from the first input point and then flows to the main input point, the main input point is connected with the reaction input flow channel.

7. The microfluidic chip according to claim 5, wherein each of the reaction chambers is provided with a rubber conductive valve, the reaction output channel comprises a first reaction output channel, a second reaction output channel and a waste liquid output channel, the reaction chamber is provided with two output paths, and the first output path is that the reaction chamber is communicated with the waste liquid output channel through the first reaction output channel and the rubber conductive valve; the second output path is that the reaction chamber is communicated with the waste liquid output channel through the second reaction output channel and the switch valve; the waste liquid output flow channel is communicated with the waste liquid cavity.

8. The microfluidic chip according to claim 7, wherein the rubber conductive valve comprises an upper layer conductive rubber valve structure disposed on the upper layer chip and a middle layer conductive rubber valve structure disposed at a corresponding position of the middle layer chip.

9. The microfluidic chip according to claim 8, wherein the sample injection cavity comprises a sample injection port penetrating the upper chip, a middle liquid injection through hole penetrating the middle chip and disposed at a corresponding position of the middle chip, and a sample injection cavity disposed on the front surface of the lower chip, and a middle exhaust hole penetrates the middle liquid injection through hole.

10. The microfluidic chip according to claim 9, wherein the middle layer liquid filling through hole is fan-shaped, and a middle layer vent hole is formed above the middle layer liquid filling through hole in a penetrating manner; the appearance cavity of advancing is fan-shaped for the banana, including straining appearance pond and water conservancy diversion district, the liquid outlet setting in the narrow limit lateral wall in straining appearance pond, the water conservancy diversion district sets up strain the bottom in appearance pond, the top of advance appearance cavity is equipped with a plurality of bleeder vent, strain the position department of the bottom of the pool in appearance pond near wide lateral wall, correspond each the bleeder vent all is provided with a guide slot, guide slot with be provided with gaseous gathering area between the water conservancy diversion district.