CN107737615A - A kind of microfluidic device for biochemistry detection - Google Patents
A kind of microfluidic device for biochemistry detection Download PDFInfo
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- CN107737615A CN107737615A CN201710855345.9A CN201710855345A CN107737615A CN 107737615 A CN107737615 A CN 107737615A CN 201710855345 A CN201710855345 A CN 201710855345A CN 107737615 A CN107737615 A CN 107737615A
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- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
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Abstract
The present invention relates to a kind of microfluidic device for biochemistry detection, the equipment includes fluid passage chip and detection zone；The fluid passage chip is used for flow injecting, mixing, and fluid passage chip is provided with the first micropore；Mixed liquid to be detected is detected by the first micropore into detection zone；Detection plot structure includes cover plate, the first substrate, the second substrate and the 3rd substrate successively from top to bottom；Cover plate, the first substrate, the second substrate and the 3rd substrate are bonded successively, are provided with and are used for the micropore that fluid comes in and goes out, and biomolecule film is fixed between the micropore of the second substrate and the 3rd substrate；Mixed fluid measured to be checked sequentially passes through the first micropore, the 3rd micropore, biomolecule film, the second micropore, the fluid channel of the first substrate micro-channel fluid entrance into the first substrate and reacted.The microfluidic device of the present invention, detection zone run through advection after biomolecule film, realize the abundant contact and reaction of fluid and biomolecule film independently of fluid channel, fluid.
Description
Technical field
The present invention relates to microfluidic art, and in particular to a kind of microfluidic device for biochemistry detection.
Background technology
Micro-fluidic chip is one important science and technology of this century, and micro-fluidic chip is that a kind of manipulation minute yardstick fluid exists
The system flowed in minim channel or structure, it can integrate a life on a small thin slice to only several square centimeters
Thing or chemical laboratory.Micro-fluidic chip can realize being greatly decreased for sample consumption, i.e. cost is greatly reduced, and reduces sample
Product processing time, detection resolution/sensitivity is improved, the development for modern science and technology has far-reaching influence and meaning.It is micro-fluidic
The applicable field of chip is quite varied, has in fields such as medical diagnosis on disease, drug screening, environment measuring, food securities at present
It is related to.
Existing micro-fluidic chip, fixing biological molecules are as reaction zone, fluid mostly on carrier (such as substrate, film)
Reacted after flowing through reaction zone in detection zone, but the contact of such reacting fluid is not abundant enough, it is likely that influence detection effect
Fruit.
The content of the invention
To solve problems of the prior art, it is an object of the invention to provide a kind of miniflow for biochemistry detection
Equipment is controlled, microfluidic device of the invention can more fully react, and testing result is more accurate, and can realize the quantitative of fluid
System and combination assay.
To realize above-mentioned technical purpose, the present invention adopts the following technical scheme that：
A kind of microfluidic device for biochemistry detection, it is characterised in that including fluid passage chip and detection zone；It is described
Fluid passage chip is used for flow injecting, mixing, and the fluid passage chip is provided with the first micropore；Mixed liquid to be detected
Body is detected by the first micropore into detection zone；The detection plot structure includes n detection unit, n >=2；One detection
Unit includes cover plate, the first substrate, the second substrate and the 3rd substrate successively from top to bottom；First substrate is provided with fluid channel
With the second substrate while be bonded；Second substrate is provided with the second micropore；3rd substrate and the second substrate are another
Simultaneously it is bonded, and the 3rd micropore is correspondingly provided with the second micropore of the second substrate；Second substrate and the 3rd substrate it is micro-
Biomolecule film is fixed between hole；The mixed fluid measured to be checked sequentially passes through the first micropore, first detection unit
The 3rd micropore, the second micropore, the first substrate micro-channel fluid entrance enter the first substrate fluid channel；After sequentially pass through it is last
The second micropore, the 3rd micropore, the first micropore of one detection unit return to fluid passage chip and enter waste liquid pool；Complete detection.
As a further improvement on the present invention, the detection zone is made up of in parallel or series at least three detection unit；It is described
The detection plot structure of series connection, mixed fluid measured to be checked enter first detection unit through the first micropore, passed through successively afterwards
(n-1)th detection unit of crossing the 2nd ..., eventually pass through n-th of detection unit micropore return to fluid passage chip enter it is useless
Liquid pool, complete detection；The multiple detection unit detection plot structure in parallel, fluid channel is additionally provided with the 3rd substrate lower surface,
After mixed fluid measured to be checked passes through the first micropore, shunted in the fluid channel of the 3rd substrate, n-1 detection before respectively enteing
Unit, the fluid into preceding n-1 detection unit converge in the first substrate fluid channel, eventually pass through n-th detection unit
Micropore returns to fluid passage chip and enters waste liquid pool, completes detection.Using the series parallel structure of multiple detection units, it is possible to achieve
The joint inspection of many index, it can also be realized by depositing multiple biomolecule on biomolecule film.
The fluid passage chip can be conventional microfluidic control chip, including substrate, cover plate, and miniflow is etched on substrate
Road, micropore is set on cover plate, fluid-mixing enters from micropore into detection zone after the mixing of sample, buffer solution is carried out in fluid channel
Row reaction.The invention provides a kind of preferable fluid passage chip structure, it can be controlled by pressure valve and realize more accurately to enter
Sample, concrete technical scheme are as follows：
The fluid passage chip structure includes successively from top to bottom：Fixed for the control of flowpath pressure valve and detection zone
Key-course；For sample introduction and the sample introduction layer of Stress control；Miniflow channel layer for fluid control；With for sealing the close of fluid channel
Sealing；Some pressure valve, the sample introduction layer and key-course and fluid channel stressor layer are provided with fluid channel in the miniflow channel layer
Valve corresponding position is provided with through hole, and key-course controls flow injecting and mixing by through hole control pressure threshold switch break-make；It is described
Window is offered on key-course, fixed test area, the sample introduction layer and the second micropore of detection zone corresponding position are provided with first at window
Micropore, fluid after being mixed in fluid channel fluid measured to be checked by the first micropore enter detection zone in detect.Chips of the present invention
Material can be thermoplastic, elastic caoutchouc, silicon or glass.
As a further improvement on the present invention, the sample introduction layer connects provided with sample inlet pool, buffer pool, waste liquid pool and outside
Mouthful；The sample inlet pool, buffer pool, waste liquid pool and external interface connect the fluid channel of miniflow channel layer；
The fluid channel of miniflow channel layer extends first, second Liang Tiao fluid channels branch from sample inlet pool and buffer pool, so
After converge, after the fluid channel after converging extends a distance into, be separated into the three, the 4th Liang Tiao fluid channels branches once again, finally exist
Converge in exit；Described 3rd, the 5th fluid channel branch extended in the 4th fluid channel branch and external interface converges for the
Six fluid channel branches；Described first, second, third, fourth and the 6th is respectively equipped with pressure valve in fluid channel branch.Pass through control
The break-make combination of five pressure valve, realizes the control of sample size according to demand.
As a further improvement on the present invention, the pressure valve arrangement is：The one of fluid channel is not set in miniflow channel layer chip
Face sets cavity, and the cavity is connected by the 4th micropore with fluid channel, and the cavity is closed using elastic film, by bullet
Property film on apply external force, block the 4th micropore, block micro-channel fluid flowing.The material selection elastomer silicone of valve, rubber
Glue, thermoplastic elastomer (TPE) or any plastic sheeting；External force is applied through key-course Mechanical course and realizes that pressure valve is opened and closed, Ke Yijing
Really the now quantitative control of fluid.
As a further improvement on the present invention, the preparation method step of the detection zone is as follows：
(1) routine techniques is used, fluid channel is set in the one side of the first substrate；Set on the second substrate and the 3rd substrate
Micropore；
(2) fixing biological molecules on large area film, then large area film is cut into the film unit for securing biomolecule,
As biomolecule film；
(3) cover plate, the first substrate, the second substrate and the 3rd substrate are bonded successively from bottom to up, biomolecule film with
Embedded form is fixed between the second substrate and the 3rd substrate；Assembling is fixed to key-course afterwards.
As a further improvement on the present invention, multiple biomolecule are fixed on the biomolecule film.Described biology
Molecule can be DNA, enzyme, antibody etc., and the joint inspection of many index can be achieved.
As a further improvement on the present invention, the biomolecule film is perforated membrane；Its material is liquid film such as water-setting
Glue, or solid film such as nitrocellulose filter, cellular glass, apertured polymeric film, porous silicon or tunica fibrosa (such as glass fibre, paper
Open, fabric).
The microfluidic device of the present invention has the following advantages that：
(1) microfluidic device of the invention, detection zone are separately provided detection zone chip, are easy to basis independently of fluid channel
Need to change detection zone, realize fluid control and the separation of reaction zone controls.
(2) microfluidic device of the invention, detection zone fluid run through advection after biomolecule film, and this structure design can
The abundant contact and mixing of fluid and biomolecule film are realized, is reacted more thorough.It can also pass through multiple detection unit strings simultaneously
Mode in parallel, realizes the joint inspection of many index.
(3) microfluidic device of the invention, avoid and complicated surface modification process is carried out to micro-fluidic chip, and use
Perforated membrane carries out biomolecule fixation, to solve the problems, such as that micro-fluidic chip surface biomolecules binding ability itself is low.
(4) microfluidic device of the invention, valve break-make can be utilized, controls sample injection volume, make accurate, reappearance
Good analysis, and effective clinical decision is made accordingly.
Brief description of the drawings
Fig. 1 is the microfluidic device fractionation structural representation of the embodiment of the present invention 1；
Fig. 2 is the microfluidic device plan cross-section structural representation of the embodiment of the present invention 1；
Fig. 3 is the detection zone fractionation structural representation of the embodiment of the present invention 1；
Fig. 4 a-b are detection unit series connection and the parallel-connection structure schematic diagram of the embodiment of the present invention 2；
Fig. 5 a-e are the use state figure of the microfluidic device of the embodiment of the present invention 1；
Embodiment
Explanation and embodiment are further described to technical scheme below in conjunction with the accompanying drawings.
Embodiment 1
The present embodiment illustrates the structure of microfluidic device of the present invention.
As Figure 1-3, microfluidic device of the invention includes fluid passage chip and detection zone 1；The fluid passage
Chip is used for flow injecting, mixing, and the fluid passage chip is provided with the first micropore 31；Mixed liquid to be detected passes through
First micropore 31 detects into detection zone 1.
As Figure 1-3, plot structure is detected described in the present embodiment includes two detection units.
Fig. 3 is that the detection plot structure for including two detection units splits schematic diagram, the detection plot structure from top to bottom according to
It is secondary including cover plate 11, the first substrate 12, the second substrate 13 and the 3rd substrate 14；First substrate 12 is provided with the one side of fluid channel
It is bonded with the one side of the second substrate 13；Second substrate 13 is provided with the second micropore 131；3rd substrate 14 and the second base
The another side of piece 13 is bonded, and is correspondingly provided with the 3rd micropore 141 at the second micropore 131 of the second substrate 13；Second substrate
13 and the 3rd substrate 14 micropore between be fixed with biomolecule film 15；The mixed fluid measured to be checked sequentially passes through first
The 3rd micropore 131, biomolecule film 15, the second micropore 131, the miniflow of the first substrate 12 of 31, first detection units of micropore
Road fluid intake enters the fluid channel of the first substrate 12；The second micropore 132, the biology of last detection unit are sequentially passed through afterwards
Molecule film 15, the 3rd micropore 142, the first micropore 31 return to fluid passage chip and enter waste liquid pool；Complete detection；
It is perforated membrane on the biomolecule film；Its material is liquid film or solid film, on a biomolecule film
Multiple biomolecule can be fixed.
The fluid passage chip can be conventional microfluidic control chip, including substrate, cover plate, and miniflow is etched on substrate
Road, micropore is set on cover plate, fluid-mixing enters from micropore into detection zone after the mixing of sample, buffer solution is carried out in fluid channel
Row reaction.A kind of preferable fluid passage chip structure is present embodiments provided, by pressure valve realization can be controlled more accurate
Sample introduction, concrete technical scheme are as follows：
The fluid passage chip structure includes successively from top to bottom：Fixed for the control of flowpath pressure valve and detection zone 1
Key-course 2；For sample introduction and the sample introduction layer 3 of Stress control；Miniflow channel layer 4 for fluid control；With for sealing miniflow
The sealant 5 in road；Be provided with some pressure valve in fluid channel in the miniflow channel layer 4, the sample introduction layer 3 and key-course 2 with it is micro-
Flow channel layer pressure valve corresponding position is provided with through hole, and key-course 2 is by through hole control pressure threshold switch break-make, to control fluid to enter
Sample and mixing；Offer window on the key-course 2, fixed test area 1 at window, the sample introduction layer 3 and detection zone 1 second are micro-
The corresponding position of hole 131 is provided with the first micropore 31, fluid after being mixed in fluid channel fluid measured to be checked pass through the first micropore 31 and enter inspection
Survey in area 1 and detect.
The sample introduction layer 3 is provided with sample inlet pool 32, buffer pool 33, waste liquid pool 34 and external interface 35；The sample inlet pool 32,
Buffer pool 33, waste liquid pool 34 and external interface 35 connect the fluid channel of miniflow channel layer 4；
The fluid channel of miniflow channel layer 4 extend from sample inlet pool and buffer pool first, second Liang Tiao fluid channels branch 41,
42, then converge, after the fluid channel after converging extends a distance into, be separated into the three, the 4th two articles of 43,44 points of fluid channels once again
Branch, finally converges in exit；Three, the 4th fluid channel branch 43,44 and the 5th miniflow extended in external interface
Road branch 45 converges for the 6th fluid channel branch 46；Described first, second, third, fourth and the 6th fluid channel branch 41-44,
Pressure valve 61-65 is respectively equipped with 46.By controlling five pressure valve 61-65 break-make to combine, sample size is realized according to demand
Control.
The pressure valve arrangement is：The one side for not setting fluid channel in the chip of miniflow channel layer 4 sets cavity, and the cavity passes through
4th micropore is connected with fluid channel, and the cavity is closed using elastic film, by applying external force on elastic film, blocks the
Four micropores, block micro-channel fluid flowing.
Embodiment 2
The present embodiment the difference is that only that detection zone includes at least three detection unit, multiple detections with embodiment 1
Unit is connected by way of serial or parallel connection.
As shown in figure 4, Fig. 4 (a) is the detection plot structure of the series connection, mixed fluid measured to be checked passes through the first micropore
Into first detection unit, pass through the 2nd successively afterwards ... and (n-1)th detection unit, eventually pass through n-th of detection unit
Micropore return to fluid passage chip and enter waste liquid pool, complete detection；
Fig. 4 (b) is the multiple detection unit detection plot structure in parallel, and miniflow is additionally provided with the 3rd substrate lower surface
Road, after mixed fluid measured to be checked passes through the first micropore, shunted in the fluid channel of the 3rd substrate, n-1 before respectively enteing
Detection unit, the fluid into preceding n-1 detection unit converge in the first substrate fluid channel, eventually pass through n-th of detection list
The micropore of member returns to fluid passage chip and enters waste liquid pool, completes detection.
Embodiment 3
This example demonstrates that the preparation method of detection zone 1 described in embodiment 1, its step are as follows：
(1) routine techniques is used, fluid channel is set in the one side of the first substrate；Set on the second substrate and the 3rd substrate
Micropore；
(2) fixing biological molecules on large area film, then large area film is cut into the film unit for securing biomolecule,
As biomolecule film；
(3) cover plate, the first substrate, the second substrate and the 3rd substrate are bonded successively from bottom to up, biomolecule film with
Embedded form is fixed between the second substrate and the 3rd substrate, and assembling afterwards is fixed to key-course.
Embodiment 4
A kind of this example demonstrates that concrete mode that the micro-fluidic chip of embodiment 1 is applied in immunoassay.
It is testing sample as shown in Fig. 5 a-b, in sample inlet pool 32, is buffer solution in buffer pool 33, passes through in the present embodiment
External interface pressure and fluid velocity are controlled, ensures that molecular screen membrane sealing is non-leakage, setting pressure is 0.01~1PSL, and flow velocity is
1~50 μ L/min.Valve 62,64 is opened, closes valve 61,63,65, in the presence of pump at external interface 35, sample flows into
One branch of fluid channel.As shown in Figure 5 c, close valve 62,64, Open valve 61,63, pump reversal effect power, by air and
Sample flow is pumped into buffer pool 33 from another branch.As fig 5d, treat that buffer solution mixes in sample flow and buffer pool, uses
Pump draws back mixed liquor, and fluid-mixing enters fluid channel branch 45；As depicted in fig. 5e, closing valve 61-64, Open valve 65,
Pump reversal effect power, fluid-mixing is pushed into detection zone, fluid is reacted by the first micropore 31 into detection zone 1.Such as figure
Shown in 5e, after having reacted, unnecessary sample waste enters miniflow channel layer 4 from the first micropore 31, into waste liquid pool 34, avoids polluting
Chipset.Being provided for of valve controls sample size, can according to sample introduction needs, by fluid channel different designs between valve,
Control the amount of response sample.
Claims (9)
1. a kind of microfluidic device for biochemistry detection, it is characterised in that including fluid passage chip and detection zone；The stream
Body channel chip is used for flow injecting, mixing, and the fluid passage chip is provided with the first micropore；Mixed liquid to be detected
Detected by the first micropore into detection zone；
The detection plot structure includes n detection unit, n >=2；
One detection unit includes cover plate, the first substrate, the second substrate and the 3rd substrate successively from top to bottom；
First substrate be provided with fluid channel with the second substrate while be bonded；It is micro- that second substrate is provided with second
Hole；
3rd substrate is bonded with the second substrate another side, and it is micro- at the second micropore of the second substrate to be correspondingly provided with the 3rd
Hole；
Biomolecule film is fixed between the micropore of second substrate and the 3rd substrate；
The mixed fluid measured to be checked sequentially pass through the first micropore, the 3rd micropore of first detection unit, the second micropore,
Into the fluid channel of the first substrate；The second micropore, the 3rd micropore, the first micropore for sequentially passing through last detection unit afterwards return
Enter waste liquid pool to fluid passage chip；Complete detection.
2. microfluidic device according to claim 1, it is characterised in that the detection zone by least three detection unit simultaneously
Join or be composed in series；
The detection plot structure of the series connection, mixed fluid measured to be checked enter first detection unit through the first micropore, it
Passing through the 2nd successively afterwards ..., (n-1)th detection unit, the micropore for eventually passing through n-th of detection unit return to fluid passage core
Piece enters waste liquid pool, completes detection；
The multiple detection unit detection plot structure in parallel, fluid channel is additionally provided with the 3rd substrate lower surface, mixed to treat
After detection fluid passes through the first micropore, shunt, n-1 detection unit before respectively enteing, enter in the fluid channel of the 3rd substrate
The fluid of preceding n-1 detection unit converges in the first substrate fluid channel, and the micropore for eventually passing through n-th of detection unit returns to stream
Body channel chip enters waste liquid pool, completes detection.
3. microfluidic device according to claim 1, it is characterised in that the fluid passage chip structure from top to bottom according to
It is secondary including：
The key-course fixed for the control of flowpath pressure valve and detection zone；
For sample introduction and the sample introduction layer of Stress control；
Miniflow channel layer for fluid control；
With the sealant for sealing fluid channel；
Some pressure valve, the sample introduction layer and key-course and fluid channel stressor layer valve are provided with fluid channel in the miniflow channel layer
Corresponding position is provided with through hole, and key-course controls flow injecting and mixing by through hole control pressure threshold switch break-make；
Offer window on the key-course, fixed test area at window, the sample introduction layer and the second micropore of detection zone corresponding position
Provided with the first micropore, fluid after being mixed in fluid channel fluid measured to be checked by the first micropore enter detection zone in detect.
4. microfluidic device according to claim 3, it is characterised in that the sample introduction layer be provided with sample inlet pool, buffer pool,
Waste liquid pool and external interface；The sample inlet pool, buffer pool, waste liquid pool and external interface connect the fluid channel of miniflow channel layer；
The fluid channel of miniflow channel layer extends first, second Liang Tiao fluid channels branch from sample inlet pool and buffer pool, then converges
Close, after the fluid channel after converging extends a distance into, be separated into the three, the 4th Liang Tiao fluid channels branches once again, finally exporting
Converge at place；Three, the 4th fluid channel branch converges micro- for the 6th with the 5th fluid channel branch extended in external interface
Runner branches；Described first, second, third, fourth and the 6th is respectively equipped with pressure valve in fluid channel branch.
5. the microfluidic device according to claim 3 or 4, it is characterised in that the pressure valve arrangement is：In miniflow channel layer
The one side that chip does not set fluid channel sets cavity, and the cavity is connected by the 4th micropore with fluid channel, and the cavity uses bullet
Property film closing, by applying external force on elastic film, block the 4th micropore, block micro-channel fluid flowing.
6. micro-fluidic chip equipment according to claim 1, it is characterised in that the preparation method step of the detection zone is such as
Under：
(1) routine techniques is used, fluid channel is set in the one side of the first substrate；Set on the second substrate and the 3rd substrate micro-
Hole；
(2) fixing biological molecules on large area film, then large area film is cut into the film unit for securing biomolecule, as
Biomolecule film；
(3) cover plate, the first substrate, the second substrate and the 3rd substrate are bonded successively from bottom to up, biomolecule film is with insertion
Form be fixed between the second substrate and the 3rd substrate；Assembling is fixed to key-course afterwards.
7. microfluidic device according to claim 1, it is characterised in that fix multiple biologies on the biomolecule film
Molecule.
8. microfluidic device according to claim 1, it is characterised in that the biomolecule film is perforated membrane.
9. application of any one of the claim 1-8 microfluidic devices in biochemistry detection.
Priority Applications (1)
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108587860A (en) * | 2018-04-24 | 2018-09-28 | 齐齐哈尔医学院 | Micro-fluidic chip combination unit and its preparation method and application for screening breast cancer cell |
| CN109234157A (en) * | 2018-09-28 | 2019-01-18 | 杨帆 | A kind of biological reaction container |
| CN109967138A (en) * | 2018-12-11 | 2019-07-05 | 安博特纳米生物科技有限公司 | Biological detection platform |
| CN111036317A (en) * | 2019-12-26 | 2020-04-21 | 厦门天马微电子有限公司 | Microfluidic chip and driving method thereof |
| CN112403539A (en) * | 2019-08-23 | 2021-02-26 | 无锡源清天木生物科技有限公司 | Micro-fluidic chip |
| CN112657565A (en) * | 2020-12-17 | 2021-04-16 | 京东方科技集团股份有限公司 | Microfluidic channel, control method thereof, microfluidic chip and analysis device |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH1164279A (en) * | 1997-08-27 | 1999-03-05 | Shimadzu Corp | Microchip electrophoresis apparatus |
| CN1932497A (en) * | 2006-10-13 | 2007-03-21 | 中山大学 | Micro-total analysis system non-contact electric conductivity detecting method and apparatus |
| CN101623660A (en) * | 2008-07-09 | 2010-01-13 | 微点生物技术有限公司 | Analysis spraying canister having liquid stream control |
| US20130096031A1 (en) * | 2011-10-13 | 2013-04-18 | Chin-Feng Wan | Microfluidic chip |
| CN106179545A (en) * | 2016-07-14 | 2016-12-07 | 南京爱思唯志生物科技有限公司 | Micro-fluidic chip equipment for bioanalysis and preparation method thereof |
| WO2017061986A1 (en) * | 2015-10-06 | 2017-04-13 | Halliburton Energy Services, Inc. | Methods and systems using micro-photomultiplier tubes and microfluidics with integrated computational elements |
-
2017
- 2017-09-20 CN CN201710855345.9A patent/CN107737615B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH1164279A (en) * | 1997-08-27 | 1999-03-05 | Shimadzu Corp | Microchip electrophoresis apparatus |
| CN1932497A (en) * | 2006-10-13 | 2007-03-21 | 中山大学 | Micro-total analysis system non-contact electric conductivity detecting method and apparatus |
| CN101623660A (en) * | 2008-07-09 | 2010-01-13 | 微点生物技术有限公司 | Analysis spraying canister having liquid stream control |
| US20130096031A1 (en) * | 2011-10-13 | 2013-04-18 | Chin-Feng Wan | Microfluidic chip |
| WO2017061986A1 (en) * | 2015-10-06 | 2017-04-13 | Halliburton Energy Services, Inc. | Methods and systems using micro-photomultiplier tubes and microfluidics with integrated computational elements |
| CN106179545A (en) * | 2016-07-14 | 2016-12-07 | 南京爱思唯志生物科技有限公司 | Micro-fluidic chip equipment for bioanalysis and preparation method thereof |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108587860A (en) * | 2018-04-24 | 2018-09-28 | 齐齐哈尔医学院 | Micro-fluidic chip combination unit and its preparation method and application for screening breast cancer cell |
| CN109234157A (en) * | 2018-09-28 | 2019-01-18 | 杨帆 | A kind of biological reaction container |
| CN109234157B (en) * | 2018-09-28 | 2021-08-27 | 杭州莱约科技有限公司 | Biological reaction container |
| CN109967138A (en) * | 2018-12-11 | 2019-07-05 | 安博特纳米生物科技有限公司 | Biological detection platform |
| CN109967138B (en) * | 2018-12-11 | 2021-11-05 | 上海微瑞博生物科技有限公司 | Biological detection platform |
| CN112403539A (en) * | 2019-08-23 | 2021-02-26 | 无锡源清天木生物科技有限公司 | Micro-fluidic chip |
| CN111036317A (en) * | 2019-12-26 | 2020-04-21 | 厦门天马微电子有限公司 | Microfluidic chip and driving method thereof |
| CN111036317B (en) * | 2019-12-26 | 2021-09-17 | 厦门天马微电子有限公司 | Microfluidic chip and driving method thereof |
| CN112657565A (en) * | 2020-12-17 | 2021-04-16 | 京东方科技集团股份有限公司 | Microfluidic channel, control method thereof, microfluidic chip and analysis device |
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