CN109967138B - Biological detection platform - Google Patents
Biological detection platform Download PDFInfo
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- CN109967138B CN109967138B CN201811512802.5A CN201811512802A CN109967138B CN 109967138 B CN109967138 B CN 109967138B CN 201811512802 A CN201811512802 A CN 201811512802A CN 109967138 B CN109967138 B CN 109967138B
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- 238000001514 detection method Methods 0.000 title claims abstract description 38
- 238000012360 testing method Methods 0.000 claims abstract description 82
- 239000007853 buffer solution Substances 0.000 claims abstract description 41
- 239000000872 buffer Substances 0.000 claims description 40
- 239000007788 liquid Substances 0.000 claims description 27
- 239000002699 waste material Substances 0.000 claims description 9
- 239000002245 particle Substances 0.000 claims description 6
- 208000035143 Bacterial infection Diseases 0.000 abstract description 4
- 208000036142 Viral infection Diseases 0.000 abstract description 4
- 230000001580 bacterial effect Effects 0.000 abstract description 4
- 208000022362 bacterial infectious disease Diseases 0.000 abstract description 4
- 230000009385 viral infection Effects 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 238000000926 separation method Methods 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 75
- 238000010586 diagram Methods 0.000 description 12
- 239000008280 blood Substances 0.000 description 9
- 210000004369 blood Anatomy 0.000 description 7
- 239000012530 fluid Substances 0.000 description 6
- 239000012472 biological sample Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 210000000601 blood cell Anatomy 0.000 description 3
- 238000011109 contamination Methods 0.000 description 3
- 239000003292 glue Substances 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000000502 dialysis Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 210000003296 saliva Anatomy 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000090 biomarker Substances 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000013013 elastic material Substances 0.000 description 1
- -1 for example Substances 0.000 description 1
- 210000004051 gastric juice Anatomy 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000012113 quantitative test Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention provides a biological detection platform, which comprises an upper cover assembly, a lower cover assembly and a buffer solution inlet, wherein the upper cover assembly is provided with an inlet for a sample and a buffer solution to flow in; the flow channel assembly is provided with a flow channel for flowing of a sample and a buffer solution; the test board is communicated with the flow channel and is used for detecting a sample; the bottom plate is detachably connected with the upper cover assembly; wherein, the upper cover component, the flow channel component, the test board and the bottom board are sequentially stacked. The biological detection platform provided by the invention has the advantages of simple structure, low production cost and convenience in disassembly and assembly, so that the upper cover assembly, the bottom plate and the test plate after disassembly and separation can not pollute the environment when being discarded, thereby avoiding causing bacterial and viral infection before incineration treatment and protecting the environment.
Description
Technical Field
The invention belongs to the technical field of biological detection devices, and particularly relates to a biological detection platform.
Background
In order to rapidly screen diseases, diagnose causes of diseases, and forensic identification, a detection instrument is required to detect biological samples such as blood, saliva, gastric juice, etc., and most quantitative tests still require trained technicians to use the biological samples in a laboratory environment. In order to facilitate patients to know their own physical condition in real time, some of the testing instruments have been reduced to simple test cartridges for patients to use at home.
However, the conventional cartridge has a complicated internal structure, and a large amount of biological samples such as blood and urine remain inside the cartridge after use, which is inconvenient to recover, and may cause bacterial and viral infections, thereby polluting the environment.
Disclosure of Invention
The invention aims to provide a biological detection platform, which aims to solve the technical problems that a biological sample is remained in a detection box, the recovery is inconvenient, and bacterial and viral infection is possibly caused in the prior art.
In order to achieve the purpose, the invention adopts the technical scheme that: providing a biological testing platform comprising:
an upper cover component which is provided with an inlet for the inflow of the sample and the buffer solution;
the flow channel assembly is provided with a flow channel for flowing of a sample and a buffer solution;
the test plate is communicated with the flow channel and is used for detecting a sample;
the bottom plate is detachably connected with the upper cover assembly;
the upper cover assembly, the runner assembly, the test plate and the bottom plate are sequentially stacked.
Further, the upper cover subassembly includes upper cover plate and anti-blood dirty stopper, the entry is seted up in the upper cover plate, anti-blood dirty stopper is located in the entry.
Furthermore, a buckle is arranged on one side, facing the bottom plate, of the upper cover plate, and a clamping groove for clamping the buckle is formed in the bottom plate.
Furthermore, the number of the inlets of the upper cover plate is two, the inlets are respectively a sample inlet and a buffer solution inlet, the flow channel assembly comprises an overflow plate, the buffer solution flow channel is formed in the overflow plate, and the buffer solution inlet is communicated with the buffer solution flow channel.
Furthermore, the buffer solution flow channel comprises a buffer solution cavity opposite to the buffer solution inlet, a micro-channel communicated with the buffer solution cavity, and a waste liquid cavity communicated with the micro-channel, wherein the bottom wall of the micro-channel is provided with a reaction port for the buffer solution to flow into the test board.
Furthermore, one side of the upper cover plate, which faces the bottom plate, is also provided with a flow guide part extending into the reaction port.
Furthermore, one side of the overflow plate, which faces the test plate, is provided with an annular rib which is annularly arranged on the reaction port, and the annular rib is abutted against the test plate.
Furthermore, the flow channel assembly also comprises a filter plate for dialyzing the sample, a sample flow channel for the sample to flow is arranged on the filter plate, the inlet of the sample flow channel is communicated with the sample inlet, and the outlet of the sample flow channel is communicated with the test plate.
Further, the surface of the upper cover plate is provided with anti-skid particles.
Further, one side of the bottom plate is provided with a handheld part.
The biological detection platform provided by the invention has the beneficial effects that: compared with the prior art, the biological detection platform comprises the flow channel assembly for the inflow of the sample and the buffer solution and the test board for testing the sample, the flow channel assembly and the test board are fixed in the biological detection platform by the upper cover assembly and the bottom board, and the upper cover assembly and the bottom board are detachably connected.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the embodiments or the prior art descriptions will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without inventive exercise.
FIG. 1 is a block diagram of a biological testing platform provided in an embodiment of the present invention;
FIG. 2 is an exploded view of a biological testing platform provided in accordance with an embodiment of the present invention;
FIG. 3 is a block diagram of an anti-bloodfouling plug according to an embodiment of the present invention;
FIG. 4 is a first structural diagram of an upper cover plate according to an embodiment of the present invention;
fig. 5 is a second structural diagram of the upper cover plate according to the embodiment of the present invention;
FIG. 6 is a first block diagram of an overflow plate according to an embodiment of the present invention;
FIG. 7 is a second block diagram of an overflow plate according to an embodiment of the present invention;
FIG. 8 is a block diagram of a first filter board according to an embodiment of the present invention;
FIG. 9 is a block diagram of a second filter board according to an embodiment of the present invention;
FIG. 10 is a block diagram of a third filter board according to an embodiment of the present invention;
FIG. 11 is a block diagram of a test board according to an embodiment of the present invention;
FIG. 12 is a first block diagram of a base plate according to an embodiment of the present invention;
fig. 13 is a second structural diagram of a base plate according to an embodiment of the present invention.
Wherein, in the figures, the respective reference numerals:
100-an upper cover assembly; 1-anti-blood-stain plug; 11-a plug; 12-a hand-pulling part; 2-upper cover plate; 201-buffer inlet; 202-a sample inlet; 203-vent hole; 204-avoidance slot; 205-a first snap hole; 21-buckling; 22-anti-slip particles; 23-a flow guide part; 300-a flow channel assembly; 3-an overflow plate; 301-avoiding holes; 302-a second snap hole; 31-buffer chamber; 311-a push port; 312-flow guiding micro-column; 32-micro flow channel; 3201-reaction port; 3202-sample inflow well; 321-a first flow blocking boss; 322-a non-return valve; 323-a second flow-impeding boss; 33-waste liquid chamber; 34-an annular rib; 4-a filter plate; 401-sample flow channel; 5-a test board; 51-a detection part; 52-signal contacts; 6-a bottom plate; 601-a card slot; 602-a handpiece; 603-positioning grooves; 61-a limiting part.
Detailed Description
In order to make the technical problems, technical solutions and advantageous effects to be solved by the present invention more clearly apparent, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or be indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or be indirectly connected to the other element.
It will be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like, as used herein, refer to an orientation or positional relationship indicated in the drawings that is solely for the purpose of facilitating the description and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and is therefore not to be construed as limiting the invention.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
Referring to fig. 1 and 2, a biological testing platform according to the present invention will now be described. The biological detection platform comprises an upper cover assembly 100, a flow channel assembly 300, a test plate 5 and a bottom plate 6, wherein the upper cover assembly 100, the flow channel assembly 300, the test plate 5 and the bottom plate 6 are sequentially stacked. The top cover assembly 100 has an inlet for the sample and the buffer solution to flow into, the flow channel assembly 300 has a flow channel for the sample and the buffer solution to flow through, the test board 5 is connected to the flow channel, and the test board 5 is used for detecting the sample. The top cover assembly 100 abuts the flow channel assembly 300 to form a seal for the flow channel. The sample and the buffer solution enter the flow channel assembly 300 through the inlet, the buffer solution cleans the test board 5 when flowing into the test board 5, and the test board 5 tests the sample when the sample flows into the test board 5. Wherein the sample can be blood, urine, saliva, etc. The test plate 5 can be selected according to the specific detection function required, and can detect proteins, small molecules, nucleic acids, biomarkers, etc. in the biological sample. The upper cover assembly 100 is detachably connected with the bottom plate 6, the flow channel assembly 300 and the test plate 5 are clamped between the upper cover assembly 100 and the bottom plate 6, before testing, a buffer solution enters the flow channel assembly 300 through an inlet of the upper cover assembly 100 and then enters the test plate 5 to wash the test plate 5, after washing is completed, a sample enters the test plate 5, the test plate 5 detects the sample, and after detection is completed, the buffer solution is used for washing the test plate 5. In this embodiment, the cover assembly 100 and the base plate 6 are detachable, and after the cover assembly 100, the flow channel assembly 300, the test board 5, the base plate 6, etc. are detached, they can be separately collected and stored.
Compared with the prior art, the biological detection platform provided by the invention comprises the runner assembly 300 for inflow of samples and buffer solution and the test board 5 for testing the samples, the runner assembly 300 and the test board 5 are fixed in the biological detection platform by the upper cover assembly 100 and the bottom board 6, the upper cover assembly 100 and the bottom board 6 are detachably connected, after the biological detection platform is used, the upper cover assembly 100 and the bottom board 6 can be detached, and the biological detection platform has the advantages of simple structure, low production cost and convenient disassembly and assembly, so that the upper cover assembly 100, the bottom board 6 and the test board 5 which are detached cannot pollute the environment when being discarded, thereby avoiding bacterial and viral infection before incineration treatment and protecting the environment.
In one embodiment, referring to fig. 2 to 4, as an embodiment of the biological detection platform provided by the present invention, the upper cover assembly 100 includes an upper cover plate 2 and an anti-blood-contamination plug 1, the inlet is disposed on the upper cover plate 2, and the anti-blood-contamination plug 1 is disposed in the inlet. The anti-blood-contamination plug 1 is made of elastic materials such as soft glue, and can seal an inlet when being plugged into the inlet, so that the sample is prevented from polluting other parts during sample injection, and gas leakage during gas injection is prevented. The side of the anti-blood-stain plug 1 is provided with a hand-pulling part 12, so that the anti-blood-stain plug is convenient to pull or plug, and is convenient for user operation and user experience improvement. The upper cover plate 2 is provided with the inlet, the buffer solution and the sample directly enter the flow channel of the flow channel assembly 300 from the inlet, and the upper cover plate 2 is abutted against the flow channel assembly 300, so that the top of the flow channel is sealed by the upper cover plate 2.
In one embodiment, referring to fig. 3 to 7, as a specific implementation manner of the biological detection platform provided by the present invention, the number of the inlets of the upper cover plate 2 is two, which are respectively a sample inlet 202 and a buffer inlet 201, the flow channel assembly 300 includes an overflow plate 3, the overflow plate 3 is provided with a buffer flow channel, and the buffer inlet 201 is communicated with the buffer flow channel. The overflow plate 3 may be made of soft glue. In this embodiment, the anti-blood-fouling plug 1 has two plugs 11, which are plugged into the sample inlet 202 and the buffer inlet 201, respectively. The sample inlet 202 is used for sample, the buffer inlet 201 is used for buffer, the buffer inlet 201 is communicated with the buffer flow channel, the buffer can flow into the buffer flow channel through the buffer inlet 201, and then the test plate 5 is washed, etc.
In one embodiment, referring to fig. 2 and 8, as an embodiment of the biological detection platform provided by the present invention, the flow channel assembly 300 further includes a filter plate 4 for dialyzing the sample, the filter plate 4 is provided with a sample flow channel 401 for flowing the sample, an inlet of the sample flow channel 401 is communicated with the sample inlet 202, and an outlet of the sample flow channel 401 is communicated with the test plate 5. The buffer flow channel and the sample flow channel 401 together constitute the flow channel described above. The filter plate 4 functions to accumulate blood cells in the sample flow channel 401 and dialyze cells irrelevant to the item to be detected when the sample is a dialysis sample, for example, blood. The sample enters the filter plate 4 through the inlet, enters the test board 5 after being dialyzed by the filter plate 4, and is tested.
Optionally, the overflow plate 3 of the flow channel assembly 300 is abutted to the upper cover plate 2, the filter plate 4 of the flow channel assembly 300 is abutted to the overflow plate 3, the filter plate 4 and the test plate 5 are disposed on the same side of the overflow plate 3, and the overflow plate 3 is disposed between the upper cover plate 2 and the test plate 5. So, when the sample got into filter 4, need pass through sample entry 202, pass overflow plate 3, seted up on the overflow plate 3 and dodged hole 301, dodged hole 301 and sample entry 202 and sample runner 401's entry just right mutually, and the sample gets into in the sample runner 401 through this dodge hole 301.
Referring to fig. 8, the filter plate 4 has a sample flow channel 401, and the sample flow channel 401 may be S-shaped. Further, the sample flow channel 401 may have an inverted structure, i.e., the width of the sample flow channel 401 gradually increases from the inlet to the outlet thereof, so as to increase the sedimentation rate of the blood cells. The filter 4 is wholly selected to be made by transparent or translucent material's material, conveniently observes the condition in the filter 4. In another embodiment, referring to fig. 9, the filter plate 4a has a sample flow channel 401a and further includes a storage chamber 402 communicating with the flow channel for storing residual accumulated blood cells and the like. In another embodiment, referring to fig. 10, the filter plate 4b has a sample channel 401b and a sample channel 401c, the inlets and outlets of the sample channel 401b and the sample channel 401c are the same, and the design of the plurality of sample channels 401 can improve the efficiency and effect of sample dialysis. The structure and number of the sample flow paths 401 are not limited herein, and include, but are not limited to, the three cases described above. Alternatively, the sample flow channel 401 may be quantitatively stored in a volume of 40 μ l to 60 μ l.
In one embodiment, referring to fig. 5 to 13, as a specific implementation of the biological detection platform provided by the present invention, a buckle 21 is disposed on a side of the upper cover plate 2 facing the bottom plate 6, and a slot 601 for the buckle 21 to be buckled is disposed on the bottom plate 6. Buckle 21 of upper cover plate 2 passes this biological detection platform's inner space and bottom plate 6's draw-in groove 601 joint, and when upper cover plate 2 and bottom plate 6 after needs are dismantled, stir buckle 21 and can make upper cover plate 2 and bottom plate 6 break away from, demolish overflow plate 3, filter 4 and survey test panel 5. Specifically, the slot 601 is a through slot, so that a user can pull the buckle 21 outside the bottom plate 6. Alternatively, the extension direction of the catch 21 is perpendicular to the plane of the upper cover plate 2, so that the catch 21 can bear stronger acting force in the vertical direction. In one embodiment, the overflow plate 3 is provided with a first fastening hole 205, the test board 5 is provided with a second fastening hole 302, and the fastener 21 passes through the first fastening hole 205 of the overflow plate 3 and the second fastening hole 302 of the test board 5 to be fastened with the fastening slot 601; in another embodiment, the buckle 21 passes through the overflow plate 3 and the filter plate 4 to be clamped with the buckle 21; in another embodiment, the overflow plate 3 is not provided with the first snap-in hole 205, and the snap 21 passes through the edge of the overflow plate 3 to be snapped into the bottom plate 6. The number of the catches 21 may be selected to be plural, and the distribution of the catches 21 is not limited herein.
Optionally, the bottom plate 6 has a plurality of limiting portions 61 on one side facing the upper cover plate 2, the limiting portions 61 are used for positioning the filter plate 4, the test plate 5 and the overflow plate 3, and each limiting portion 61 is respectively penetrated through the filter plate 4, the test plate 5 and the overflow plate 3. For example, part of the limiting part 61 sequentially passes through the filter plate 4 and the overflow plate 3, and part of the limiting part 61 sequentially passes through the test plate 5 and the overflow plate 3, so that the filter plate 4, the test plate 5 and the overflow plate 3 are positioned. The stopper 61 may be cylindrical, and the number thereof is not limited herein.
In one embodiment, referring to fig. 6 and 7, as an embodiment of the biological detection platform provided by the present invention, the buffer fluid channel includes a buffer fluid chamber 31 opposite to the buffer fluid inlet 201, a micro-channel 32 communicating with the buffer fluid chamber 31, and a waste fluid chamber 33 communicating with the micro-channel 32, wherein a reaction port 3201 for allowing the buffer fluid to flow into the test board 5 is formed on a bottom wall of the micro-channel 32. After entering the buffer chamber 31, the buffer solution flows into the micro flow channel 32, flows into the test board 5 through the reaction port 3201, washes the test board 5, and flows into the waste chamber 33. Optionally, the buffer chamber 31 has a liquid pushing port 311 at the inlet thereof, which is adapted to the buffer inlet 201, so that the side wall of the buffer chamber 31 is hermetically connected to the side wall of the buffer inlet 201. Alternatively, the number of the reaction ports 3201 is plural, and the reaction ports are distributed in order along the length direction of the micro flow channel 32.
Optionally, the inlet and the outlet of the buffer liquid cavity 31 are provided with a plurality of flow guiding micro-pillars 312, the flow guiding micro-pillars 312 are distributed at intervals, and the flow guiding micro-pillars 312 serve to increase the uniformity of the liquid flow and have a certain guiding effect on the liquid. The flow guide micro-column 312 may have a cylindrical shape with a depth less than or equal to the depth of the buffer chamber 31. When the flow guide micro-pillars 312 are cylindrical, the ratio of the height of the flow guide micro-pillars 312 to the diameter thereof is 1:1 to 1: 5, the ratio of the diameter of the flow guiding micro-pillars 312 to the distance between the adjacent flow guiding micro-pillars 312 is 1: 0.5-1: 1, and the ratio of the cross-sectional area of the flow guide micro-column 312 to the area of the buffer liquid cavity 31 is 3-15%. More specifically, the width of the inlet and the outlet of the buffer liquid chamber 31 are smaller than the width of the middle chamber, so that the capacity of the buffer liquid chamber 31 can be ensured, and the flow rate can be controlled more conveniently.
Optionally, the overflow plate 3 is provided with a sample inflow hole 3202 opposite to the outlet of the sample channel 401, and the sample inflow hole 3202 is disposed such that when the sample flows to the sample outlet, the sample enters the micro channel 32 through the sample inflow hole 3202, and then enters the test plate 5 from the reaction port 3201 at the bottom of the micro channel 32. More specifically, the sample inflow hole 3202 is provided at the inlet of the micro flow channel 32.
Optionally, the depth of the micro flow channel 32 is smaller than the depth of the buffer chamber 31, so that when the buffer chamber 31 is filled with the buffer solution, the buffer solution flows into the micro flow channel 32, firstly, the side wall of the micro flow channel 32 is washed as much as possible, secondly, the flow of the buffer solution can be accurately controlled, and the quantitative detection is convenient. The depth of the micro-channel 32 is smaller than that of the waste liquid cavity 33, so that the liquid in the waste liquid cavity 33 can not flow back towards the micro-channel 32, and the accuracy of the detection result is ensured.
Optionally, a first flow-blocking boss 321 is disposed at an inlet of the micro flow channel 32 to prevent liquid in the micro flow channel 32 from flowing back to the buffer chamber 31, the first flow-blocking boss 321 may be a protrusion in a shape of a perfect circular arc, an elliptical arc, or an arc with corners, and the height of the protrusion is higher than the depth of the micro flow channel 32, the first flow-blocking boss 321 spans two sides of the micro flow channel 32, and a ratio of the height of the first flow-blocking boss 321 to the overall height of the micro flow channel 32 is 5/10 to 9/10. The second flow-blocking boss 323 is arranged at the outlet of the micro-channel 32 to prevent the liquid in the waste liquid cavity 33 from flowing back to the micro-channel 32, the second flow-blocking boss 323 can be a protrusion with a perfect circular arc shape, an elliptical arc shape or an arc shape at the corner, the height of the protrusion is higher than the depth of the micro-channel 32, the second flow-blocking boss 323 also spans two sides of the micro-channel 32, and the ratio of the height of the second flow-blocking boss 323 to the overall height of the micro-channel 32 is 5/10-9/10.
Optionally, a check valve 322 is further disposed between the micro flow channel 32 and the buffer chamber 31, and the width of the check valve 322 is smaller than the width of the micro flow channel 32, so that the liquid in the micro flow channel 32 cannot flow back. Optionally, the ratio of the width of the non-return valve to the width of the microchannel 32 is between 1/12 and 6/12. In one embodiment, the overflow plate 3 has both the check valve 322 and the first flow-blocking protrusion 321, which can ensure that the liquid in the micro flow channel 32 does not flow back into the buffer chamber 31. Of course, in other embodiments, the overflow plate 3 may also comprise only the first flow-blocking projection 321 or the non-return valve 322.
In one embodiment, referring to fig. 7, as a specific implementation of the biological detection platform provided by the present invention, the side of the overflow plate 3 facing the test plate 5 has an annular rib 34 annularly disposed on the reaction opening 3201, the annular rib 34 abuts against the test plate 5, and the annular rib 34 functions to: and the liquid enters the channel of the test board 5 from the reaction port 3201 and is sealed by abutting against the test board 5, so that the leakage of buffer liquid is prevented, and the overflow board 3 and the test board 5 are connected in a melting way without using chemical reagents such as glue and the like during assembly, so that the disassembly is convenient. Specifically, one annular rib 34 is provided around each reaction port 3201, or one annular rib 34 is provided on the overflow plate 3, and the annular rib 34 surrounds all the reaction ports 3201. Optionally, the ratio of the height to the width of the annular rib 34 is in the range of 1:1 to 1: 5, or more. The shape of the annular rib 34 is not limited here, and may surround the reaction port 3201.
In one embodiment, referring to fig. 5, as a specific implementation of the biological detection platform provided by the present invention, the upper cover plate 2 further has a flow guiding portion 23 extending into the reaction port 3201 on a side facing the bottom plate 6, so that when the buffer solution flows into the test plate 5 through the reaction port 3201, the flow direction of the buffer solution is changed, the buffer solution is guided to flow into the test plate 5, and the cleaning effect on the test plate 5 is enhanced. The outer dimension of the flow guide 23 is smaller than the dimension of the reaction port 3201, so that the buffer solution flows into the test plate 5 from between the inner wall of the reaction port 3201 and the outer wall of the flow guide 23.
Alternatively, the inner wall of the sample inlet 202 in the upper cover plate 2 is chamfered to guide the sample into the flow channel, and more specifically, when the sample is blood, the blood can be injected into the flow channel by using an injection member such as finger tip blood and a dropper.
Optionally, the upper cover plate 2 is further provided with an exhaust hole 203, the exhaust hole 203 is opposite to the waste liquid cavity 33, so that when liquid enters the overflow plate 3, the air pressure in the buffer liquid flow channel is too high, and the liquid cannot be injected.
In one embodiment, referring to fig. 5, as a specific implementation of the biological detection platform provided by the present invention, the surface of the upper cover plate 2 has anti-slip particles 22, and the anti-slip particles 22 are composed of a plurality of protrusions. The function of the anti-slip particles 22 is: when pressing this department, can prevent that whole biological detection platform from rocking, guarantee its stability.
Optionally, the portion of the upper cover plate 2 opposite to the outlet of the sample flow channel 401 is transparent or semitransparent, so as to facilitate observation of whether the sample is fully filled in the sample flow channel 401 from the outside, and more specifically, the whole filter plate 4 may be made of a transparent or semitransparent material, so as to facilitate observation of the conditions inside the filter plate 4.
Alternatively, the test plate 5 includes the detecting parts 51 facing the reaction ports 3201, and the number of the detecting parts 51 is the same as the number of the reaction ports 3201. The test board 5 further includes a signal contact 52, and the signal contact 52 converts the detected biological signal into an electrical signal and transmits the electrical signal to an external display lamp or display for reading the detection result.
Alternatively, the signal contacts 52 are provided at the edge of the test board 5. The upper cover plate 2 is provided with an avoiding groove 204 at the opposite position of the signal contact 53, so that the signal contact 52 is arranged on the outer surface of the biological detection platform, and the signal contact 52 is convenient to be electrically connected with an external power supply or a detection device.
In one embodiment, referring to fig. 12 and 13, a hand-held portion 602 is disposed on one side of the bottom plate 6, and the hand-held portion 602 is convenient for a user to hold, thereby improving user experience. More specifically, the surface of the hand-held portion 602 is curved to fit the hand.
Optionally, referring to fig. 13, a positioning groove 603 is formed in an outer wall of the bottom plate 6, the positioning groove 603 is engaged with other components, when the entire biological detection platform is slid until the positioning groove 603 is engaged with other components, that is, a predetermined position is reached, and the positioning groove 603 is provided to facilitate a user to position the biological detection platform. The number and distribution of the positioning slots 603 are not limited herein.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (5)
1. A biological testing platform, comprising:
an upper cover component which is provided with an inlet for the inflow of the sample and the buffer solution;
the flow channel assembly is provided with a flow channel for flowing of a sample and a buffer solution;
the test plate is communicated with the flow channel and is used for detecting a sample;
the bottom plate is detachably connected with the upper cover assembly;
the upper cover assembly, the runner assembly, the test board and the bottom plate are sequentially stacked, and the runner assembly and the test board are clamped between the upper cover assembly and the bottom plate; the runner assembly comprises an overflow plate, a first clamping hole is formed in the overflow plate, a second clamping hole is formed in the test plate, and the clamp passes through the first clamping hole of the overflow plate and the second clamping hole of the test plate to be clamped with the clamping groove;
the flow channel assembly comprises an overflow plate, a buffer solution flow channel is formed in the overflow plate, and the buffer solution inlet is communicated with the buffer solution flow channel;
the flow channel assembly further comprises a filter plate for dialyzing a sample, a sample flow channel for the sample to flow is formed in the filter plate, an inlet of the sample flow channel is communicated with the sample inlet, an outlet of the sample flow channel is communicated with the test plate, and the width of the sample flow channel from the inlet to the outlet is gradually increased;
the buffer solution flow channel comprises a buffer solution cavity opposite to the buffer solution inlet, a micro-flow channel communicated with the buffer solution cavity and a waste liquid cavity communicated with the micro-flow channel, and the bottom wall of the micro-flow channel is provided with a reaction port through which the buffer solution flows into the test board; the inlet and the outlet of the buffer liquid cavity are provided with flow guide micro-columns, the number of the flow guide micro-columns is a plurality of and distributed at intervals, and the ratio of the height of each flow guide micro-column to the diameter of each flow guide micro-column is 1:1 to 1: 5, the ratio of the diameter of the flow guide micro-column to the distance between the adjacent flow guide micro-columns is 1: the ratio of the cross section area of the flow guide micro-column to the area of the buffer liquid cavity is between 3% and 15% and is between 0.5 and 1: 1; the depth of the micro-channel is less than the depth of the buffer liquid cavity.
2. The biological detection platform of claim 1, wherein the side of the upper cover plate facing the bottom plate further comprises a flow guide portion extending into the reaction port.
3. The biological detection platform of claim 1, wherein the side of the overflow plate facing the test plate has an annular rib surrounding the reaction port, the annular rib abutting against the test plate.
4. The biological testing platform of claim 1, wherein a surface of the upper deck has anti-slip particles.
5. The biological testing platform of claim 1, wherein one side of the base plate has a hand-held portion.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106119085A (en) * | 2016-08-23 | 2016-11-16 | 闫维新 | A kind of real-time fluorescence PCR mixing microchannel chip |
| CN205720234U (en) * | 2016-03-16 | 2016-11-23 | 杭州安旭科技有限公司 | A kind of collection and detection device of sample |
| CN107126753A (en) * | 2017-05-25 | 2017-09-05 | 苏州岚轩生物科技有限公司 | Active micro-fluidic chip and its Whole Blood Filtration device |
| CN107737615A (en) * | 2017-09-20 | 2018-02-27 | 南京肯辛顿诊断科技有限公司 | A kind of microfluidic device for biochemistry detection |
| CN207908375U (en) * | 2018-02-09 | 2018-09-25 | 广东达元绿洲食品安全科技股份有限公司 | A kind of cover buckle formula organophosphate and carbamate pesticide residual rapidly measuring device |
| CN209680120U (en) * | 2018-12-11 | 2019-11-26 | 上海微瑞博生物科技有限公司 | Biological detection platform |
-
2018
- 2018-12-11 CN CN201811512802.5A patent/CN109967138B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN205720234U (en) * | 2016-03-16 | 2016-11-23 | 杭州安旭科技有限公司 | A kind of collection and detection device of sample |
| CN106119085A (en) * | 2016-08-23 | 2016-11-16 | 闫维新 | A kind of real-time fluorescence PCR mixing microchannel chip |
| CN107126753A (en) * | 2017-05-25 | 2017-09-05 | 苏州岚轩生物科技有限公司 | Active micro-fluidic chip and its Whole Blood Filtration device |
| CN107737615A (en) * | 2017-09-20 | 2018-02-27 | 南京肯辛顿诊断科技有限公司 | A kind of microfluidic device for biochemistry detection |
| CN207908375U (en) * | 2018-02-09 | 2018-09-25 | 广东达元绿洲食品安全科技股份有限公司 | A kind of cover buckle formula organophosphate and carbamate pesticide residual rapidly measuring device |
| CN209680120U (en) * | 2018-12-11 | 2019-11-26 | 上海微瑞博生物科技有限公司 | Biological detection platform |
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