CN217385513U

CN217385513U - Microfluidic chip card for fluorescence immunoassay analyzer

Info

Publication number: CN217385513U
Application number: CN202122639702.2U
Authority: CN
Inventors: 王建
Original assignee: Weihai Prius Precision Electronics Co ltd
Current assignee: Weihai Prius Precision Electronics Co ltd
Priority date: 2021-11-01
Filing date: 2021-11-01
Publication date: 2022-09-06
Anticipated expiration: 2031-11-01

Abstract

The utility model relates to the technical field of biomedical detection, and discloses a micro-fluidic chip card for a fluorescence immunoassay analyzer, which solves the problems that the buffer effect of the structure in the buffer slot of the prior micro-fluidic chip card for the fluorescence immunoassay analyzer on a sample is poor, bubbles are difficult to be effectively treated and the practicability is not enough, and comprises a lower substrate and an upper substrate, wherein the upper substrate is arranged at the upper end of the lower substrate, the buffer slot is arranged at one side of the upper end of the lower substrate positioned below the upper substrate, a sample adding hole is arranged at the position on the upper substrate, which is just opposite to the buffer slot, a buffer grid is arranged on the lower substrate positioned in the buffer slot, and the micro-fluidic chip card has better buffer effect compared with the structures such as a buffer column in the prior micro-fluidic chip card by arranging the buffer grid, can ensure that the sample and the buffer agent are more fully combined, and the buffer grid can cut the bubbles generated by viscous protein, preventing air bubbles generated by viscous proteins in the sample from blocking the microfluidic channel.

Description

Microfluidic chip card for fluorescence immunoassay analyzer

Technical Field

The utility model belongs to the technical field of the biomedical detects, specifically be a micro-fluidic chip card for fluorescence immunoassay appearance.

Background

The micro-fluidic chip technology integrates basic operation units of sample preparation, reaction, separation, detection and the like in the biological, chemical and medical analysis process into a micron-scale chip, and automatically completes the whole analysis process. Due to its great potential in the fields of biology, chemistry, medicine and the like, the method has been developed into a new research field crossing the disciplines of biology, chemistry, medicine, fluid, electronics, materials, machinery and the like.

However, the existing structure in the buffer groove of the microfluidic chip card for the fluorescence immunoassay analyzer has poor buffer effect on the sample, is difficult to effectively process bubbles, and has insufficient practicability.

Disclosure of Invention

To the above situation, for overcoming prior art's defect, the utility model provides a micro-fluidic chip card for fluorescence immunoassay appearance, the effectual current structure that is used for fluorescence immunoassay appearance's micro-fluidic chip card dashpot of having solved is relatively poor to the buffering effect of sample, is difficult to the problem of effective processing bubble, practicality not enough.

In order to achieve the above object, the utility model provides a following technical scheme: a micro-fluidic chip card for fluorescence immunoassay appearance, including bottom end chip and last substrate, the bottom end chip upper end is provided with go up the substrate, is located go up the substrate below bottom end chip upper end one side is provided with the dashpot, go up the substrate on just be provided with the application of sample hole to the position of dashpot, be located in the dashpot be provided with the buffering grid on the bottom end chip, dashpot one side is provided with the mark groove, in the mark groove with be provided with the mark point on the bottom end chip of dashpot hookup location, the mark groove is kept away from dashpot one side is provided with the reaction tank.

Preferably, the lower substrate upper end with the lower extreme of the upper substrate is glued joint, the dashpot shaping in the lower substrate upper end, the shaping of application of sample hole in on the upper substrate.

Preferably, the buffer grid is formed on the lower substrate in the buffer groove, and the mark groove is formed on the lower substrate.

Preferably, the mark points are formed on the lower substrate in the mark groove, and the reaction groove is formed on the lower substrate.

Preferably, a first detection point is formed on the lower substrate located in the reaction tank, a waste liquid tank is formed on the lower substrate on the side of the reaction tank away from the marking tank, and a second detection point is formed on the lower substrate located in the waste liquid tank.

Preferably, a lower through hole is formed in the lower substrate on the side, away from the reaction tank, of the waste liquid tank, and an upper through hole is formed in the position, facing the lower through hole, of the upper substrate.

Compared with the prior art, the beneficial effects of the utility model are that:

1) in the micro-fluidic chip card for the fluorescence immunoassay instrument, the buffering grating is arranged, so that the micro-fluidic chip card has a better buffering effect compared with structures such as a buffering column in the conventional micro-fluidic chip card, a sample and a buffering agent can be combined more sufficiently, meanwhile, the buffering grating can cut bubbles generated by viscous protein, the situation that the bubbles generated by the viscous protein in the sample block a micro-fluidic channel is prevented, and the problems that the buffering effect of the structure in the buffering groove of the conventional micro-fluidic chip card for the fluorescence immunoassay instrument on the sample is poor, the bubbles are difficult to treat effectively and the practicability is insufficient are solved;

2) in the micro-fluidic chip card for the fluorescence immunoassay analyzer, the reaction tank and the waste liquid tank which are contacted in a large area, and the dense first detection point and the dense second detection point are arranged, so that the reaction state of a sample can be visually observed through the color change of the large-area first detection point and the large-area second detection point when the sample reacts, and the problems that the contact area of the reaction tank and the waste liquid tank of the existing micro-fluidic chip for the fluorescence immunoassay analyzer is small, the sample flows fast in the reaction tank, the reaction time is short, and the reaction state of the sample is difficult to visually observe are solved.

Drawings

The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention, and together with the description serve to explain the invention and not to limit the invention. In the drawings:

FIG. 1 is a cross-sectional view of the present invention;

fig. 2 is a bottom view of an upper substrate of the present invention;

FIG. 3 is a top view of the middle and bottom substrates according to the present invention;

in the figure: 1. a lower substrate; 2. an upper substrate; 3. a buffer tank; 4. a sample application hole; 5. a buffer grid; 6. marking a groove; 7. marking points; 8. a reaction tank; 9. a first detection point; 10. a waste liquid tank; 11. a second detection point; 12. a lower through hole; 13. and an upper through hole.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments; based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts all belong to the protection scope of the present invention.

This embodiment, given by fig. 1-3, the utility model discloses a lower substrate 1 and upper substrate 2, lower substrate 1 upper end is provided with upper substrate 2, lower substrate 1 upper end one side that is located upper substrate 2 below is provided with dashpot 3, upper substrate 2 goes up and just is provided with application of sample hole 4 to the position of dashpot 3, be provided with buffering grid 5 on the lower substrate 1 that is located dashpot 3, dashpot 3 one side is provided with mark groove 6, be provided with mark point 7 in the mark groove 6 with the lower substrate 1 of dashpot 3 hookup location, mark groove 6 is far away from dashpot 3 one side and is provided with reaction tank 8.

Wherein, lower substrate 1 upper end and the lower extreme cementing of upper substrate 2, buffer slot 3 shaping in lower substrate 1 upper end, and the shaping of application of sample hole 4 is on upper substrate 2, can be to dripping the sample in buffer slot 3 through application of sample hole 4.

The buffer grids 5 are formed on the lower substrate 1 in the buffer groove 3, the marking groove 6 is formed on the lower substrate 1, the buffer grids 5 are densely distributed in the buffer groove 3, a sample added into the buffer groove 3 can be buffered, the sample and a buffer in a buffer area can be fully mixed, meanwhile, the buffer grids 5 can cut bubbles generated by viscous protein, the micro-fluidic channel is prevented from being blocked by the bubbles generated by the viscous protein in the sample, and then the sample mixed liquid in the buffer groove 3 flows to the marking groove 6 under the driving of a micro-pump outside the micro-fluidic chip card.

The marking point 7 is formed on the lower substrate 1 in the marking groove 6, the reaction groove 8 is formed on the lower substrate 1, and the color indicator and the antigen or antibody with immunological activity are arranged in the marking point 7, so that the flowing condition of the sample in the micro-channel can be conveniently observed after the sample is adhered with the color.

Wherein, the lower substrate 1 located in the reaction tank 8 is formed with the first detecting point 9, the lower substrate 1 of the reaction tank 8 far away from the marking slot 6 is formed with the waste liquid slot 10, the lower substrate 1 located in the waste liquid slot 10 is formed with the second detecting point 11, the first detecting point 9 and the second detecting point 11 are dried with water-soluble color indicators, the sample enters the reaction tank 8 and then undergoes biochemical reaction, the volume of the reaction tank 8 is large, the flow rate of the sample can be greatly reduced, the reaction time of the sample can be greatly prolonged, the detection sensitivity of the chip can be ensured, the sample can flow into the waste liquid slot 10 after the reaction is completed, the second detecting points 11 in the waste liquid slot 10 are more dense, the color change of the second detecting points 11 can be conveniently observed through the upper substrate 2, and then whether the sample completely reacts can be determined.

Wherein, a lower through hole 12 is formed on the lower substrate 1 which is positioned on one side of the waste liquid groove 10 far away from the reaction groove 8, an upper through hole 13 is formed on the position of the upper substrate 2 facing the lower through hole 12, the upper through hole 13 and the lower through hole 12 form a complete through hole, and waste liquid in the waste liquid groove 10 can be discharged through the complete through hole.

The working principle is as follows: the sample can be dripped into the buffer groove 3 through the sample adding hole 4, the buffer grids 5 are densely distributed in the buffer groove 3, the sample added into the buffer groove 3 can be buffered, the sample and the buffer in the buffer area can be fully mixed, meanwhile, the buffer grids 5 can cut bubbles generated by viscous protein, the micro-fluidic channel can be prevented from being blocked by the bubbles generated by the viscous protein in the sample, then, the sample mixed liquid in the buffer groove 3 flows to the marking groove 6 under the driving of a micro-fluidic chip card micro-pump outside the micro-fluidic chip card, the marking point 7 is internally provided with a color indicator and an antigen or an antibody with immune activity, thus, the flowing condition of the sample in the micro-channel can be conveniently observed after the sample is attached with color, the sample generates biochemical reaction after entering the reaction groove 8, the volume of the reaction groove 8 is larger, the flow rate of the sample can be greatly reduced, the reaction time of the sample is greatly prolonged, the detection sensitivity of the chip can be favorably ensured, and after the reaction of the sample is finished, the sample flows into the waste liquid tank 10, the second detection points 11 in the waste liquid tank 10 are more dense, the reaction condition can be observed through the color change of the dried water-soluble color indicators on the first detection point 9 and the second detection points 11, so that whether the sample is completely reacted or not is determined, and the reacted waste liquid can be discharged through the upper through hole 13 and the lower through hole 12.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. Microfluidic chip card for a fluorescence immunoassay analyzer, comprising a lower substrate (1) and an upper substrate (2), characterized in that: the lower substrate (1) upper end is provided with go up substrate (2), is located go up substrate (2) below lower substrate (1) upper end one side is provided with dashpot (3), go up substrate (2) and just be provided with application of sample hole (4) to the position of dashpot (3) on, be located in dashpot (3) be provided with buffering grid (5) on lower substrate (1), dashpot (3) one side is provided with mark groove (6), in mark groove (6) with be provided with mark point (7) on dashpot (3) hookup location's lower substrate (1), mark groove (6) are kept away from dashpot (3) one side is provided with reaction trough (8).

2. Microfluidic chip card for a fluoroimmunoassay instrument according to claim 1, characterized in that: lower substrate (1) upper end with go up substrate (2) lower extreme cementing, dashpot (3) shaping in lower substrate (1) upper end, application of sample hole (4) shaping in go up on substrate (2).

3. Microfluidic chip card for a fluoroimmunoassay instrument according to claim 1, characterized in that: the buffer grid (5) is formed on the lower substrate (1) in the buffer groove (3), and the mark groove (6) is formed on the lower substrate (1).

4. Microfluidic chip card for a fluoroimmunoassay analyzer according to claim 1, characterized in that: the marking points (7) are formed on the lower substrate (1) in the marking groove (6), and the reaction groove (8) is formed on the lower substrate (1).

5. Microfluidic chip card for a fluoroimmunoassay instrument according to claim 1, characterized in that: a first detection point (9) is formed on the lower substrate (1) in the reaction tank (8), a waste liquid tank (10) is formed on the lower substrate (1) on one side, away from the marking tank (6), of the reaction tank (8), and a second detection point (11) is formed on the lower substrate (1) in the waste liquid tank (10).

6. Microfluidic chip card for a fluoroimmunoassay instrument according to claim 5, characterized in that: a lower through hole (12) is formed in the lower substrate (1) on one side, far away from the reaction tank (8), of the waste liquid tank (10), and an upper through hole (13) is formed in the position, facing the lower through hole (12), of the upper substrate (2).