CN107199057A - Dismounting brief classifiable tumor mark joint inspection chip apparatus convenient to both - Google Patents
Dismounting brief classifiable tumor mark joint inspection chip apparatus convenient to both Download PDFInfo
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- CN107199057A CN107199057A CN201610189113.XA CN201610189113A CN107199057A CN 107199057 A CN107199057 A CN 107199057A CN 201610189113 A CN201610189113 A CN 201610189113A CN 107199057 A CN107199057 A CN 107199057A
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
- B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
Abstract
Brief classifiable tumor mark joint inspection chip apparatus convenient to both is dismounted the present invention relates to a kind of, belongs to analysis testing field.Dimethyl silicone polymer be PDMS its be used for making the substrate of the multichannel combined detection micro-fluidic chip of classifiable tumor mark, advantageous, the also serial problem of solution in need；This case is directed to the serial problem.This case main points are, the selected PDMS with ecosystem surface of substrate, and fastening is positioned at the test liquid stream terminal of the micro-fluidic chip its neighbor positions manually by the hinge-type fixture for being attached to miniature ultrasonic transducer units, simultaneously, ultrasonic wave damper is installed at the sample introduction end of the micro-fluidic chip, reach ultrasonic intensity rapid decrement in short distance, so as to form interfacial tension difference at the two ends of the chip, thereby facilitate test liquid stream to be flowed along originally hydrophobic capillary channel to terminal direction.The device is without Micropump.
Description
Technical field
Brief classifiable tumor mark joint inspection chip apparatus convenient to both is dismounted the present invention relates to a kind of, belongs to analysis test neck
Domain.
Background technology
Tumor markers (tumor marker, TM) refers in the generation and breeding of tumour, is produced in itself by tumour cell
Raw either reacted by body tumour cell and produce, reflection tumour exist and growth a class material, including protein,
Hormone, enzyme (isodynamic enzyme) and oncoprotein etc..The tumor markers in blood samples of patients or body fluid is chemically examined, can be in cancer screening
Early detection tumour, and observe the curative effect of oncotherapy and judge patient's prognosis.The tumor markers clinically commonly used at present has：
(1) alpha-fetoprotein (AFP) is the mark of the tumours such as primary carcinoma of liver, carcinoma of testis, oophoroma；(2) carcinomebryonic antigen (CEA) is digestion
The mark of the tumours such as system tumor, lung cancer, breast cancer；(3) CA125 (CA125) is the mark of the tumours such as oophoroma；
(4) CA153 (CA153) is the mark of the tumours such as breast cancer；(5) CA19-9 (CA19-9) is digestive system tumor
Mark；(6) CA724 (CA724) is the mark of the tumours such as stomach cancer, oophoroma；(7) carbohydrate antigen 242 (CA242)
For the mark of digestive system tumor；(8) CA50 (CA50) is the mark of the tumours such as digestive system tumor, breast cancer, lung cancer
Thing；(9) CYFRA21-1 (Cy211) is the mark of the tumours such as non-small cell lung cancer；(10) neuronspecific enolase (NSE)
For the mark of the tumours such as ED-SCLC, neuroendocrine tumor；(11) PSA (PSA) is prostate cancer
Tumor markers；(12) human chorionic gonadotrophin (HCG) is that embryonic cell carcinoma, trophoblastic tumor (suede cancer, vesicular mole) etc. are swollen
The mark of knurl；(13) thyroglobulin (TG) is the mark of thyroid cancer；(14) ferritin (SF) is digestive system tumor, liver
The mark of the tumours such as cancer, breast cancer, lung cancer；(15) B2M (β 2-MG) is in chronic lymphocytic leukemia, lymph
Raised in the patient body fluids such as knurl, myeloma, lung cancer, thyroid cancer, nasopharyngeal carcinoma；(16) squamous cell antigen (SCC) be cervical carcinoma,
The tumor markerses such as lung squamous cancer, the cancer of the esophagus.The tumor markers overwhelming majority clinically detected at present is not only present in malignant tumour
In, exist in benign tumour, embryonic tissue, even in normal structure.Therefore, tumor markers has dynamic chek and multinomial
Joint inspection is more valuable.So for numerous tumor markerses, clinically how to selectDifferent tumours understands some phases
To special tumor markers, such as CA153 often appears in breast cancer；CEA often appears in intestinal cancer, stomach cancer；CA19-9 is often appeared in
Intestinal cancer, cancer of pancreas；CA125 often appears in oophoroma etc..Clinician can be according to the different mark of different tumor examinations.
Same tumour or different types of tumour can have one or more of tumor markerses abnormal；Same tumor markers can be in difference
Tumour in occur.To improve the additive diagnostic value of tumor markers and determining which kind of mark can be supervised as the follow-up after treatment
Index is surveyed, tumor markers joint-detection, the complementary tumor markers group of several sensitivity of reasonable selection, specific performance can be carried out
Into best of breed, joint-detection is carried out.In general the joint-detection of tumor markers can improve the accuracy to diagnosing tumor.
Only with regard to Diagnostic Value of Several Serum Tumor Markers its joint-detection background of related itself general picture or overview for, may refer to
Lower Chinese invention patent application case：CN200410041175.3、CN200510026780.8、CN200610040051.2、
CN200910064647.X。
Only for the microfluidic chip technology overall general picture of itself, famous micro-fluidic expert Mr. Lin Ping Cheng may refer to soon
Before the monograph " diagram Microfluid based Lab on a chip " that goes out, the monograph is published via Science Press, and the monograph is for micro-fluidic
Past of technology, now, and, in terms of vision of the future etc., suffer from detail, be deep into long discussion of detail.
So, the focal issue that this case that to have a talk below is paid special attention to.
The basic framework of micro-fluidic chip, including it is etched with the substrate of small fluid course and the cover plate fit together therewith,
Small fluid course on the substrate, assembling upper cover plate before, it is apparent on see to be exactly some micro-channels, wait until thereon
After covering cover plate, just real closure forms the small fluid course, and the conduit inner surface of the micro-channel is together with surround this
The part cover plate of micro-channel constitutes described small fluid course together；So, it is clear that this after assembling is completed is small
Fluid course, the major part of its inner surface area is the inner surface area of that micro-channel, in other words, in the micro-channel
The state or property on surface substantially determine the integrality or property of the small fluid course；Therefore say, this is built in base
The inner surface state or inner surface property of micro-channel on piece are key factors；In principle, it is any to keep or protecting substantially
The material of its solid forms is held, can be used to make substrate and cover plate, such as, the material that can act as substrate and cover plate can be with
It is monocrystalline silicon piece, quartz plate, sheet glass, high polymer such as dimethyl silicone polymer, polymethyl methacrylate, makrolon etc.
Deng；Certainly, the selection of substrate and the selection of cover plate be able to can also be differed with identical；From material consumption, manufacture difficulty and
From the point of view of in terms of application popularization prospect etc., between these materials exist not small difference, the especially selection of that substrate, influence compared with
Greatly.
In various substrate making materials, dimethyl silicone polymer, i.e. PDMS, comparatively very easily shaping, so
Substrate on make that micro-channel is extremely simple, and the lower cost for material makes substrate with the polydimethyl siloxane material,
Micro-channel is being suppressed or etched thereon, and is being engaged with the cover plate that the cheap material such as glass or polypropylene or other plastic sheets makes,
It is a kind of more satisfactory selection seemingly；Certainly, patch material can also select to use cheap polydimethyl siloxane material：
So, this substrate selection is the scheme of polydimethyl siloxane material, and material is extremely cheap, makes extremely simple, seems and should also be as
It is extremely easy to popularize, promotes.
But, thing is really not so simple.
First, this polydimethyl siloxane material, that is, the material that the letter PDMS that abridges is referred to, itself are a kind of strong
Hydrophobic material, micro-channel is built on this material, if without being operated for the modified of the micro-channel surface, then,
After overall assembling is completed, that is, cover after cover plate, because the micro-channel its inner surface in structure occupies most liquid circulation
The inner surface in road, then, its strong hydrophobic property of the PDMS micro-channels inner surface, is deciding factor, it can cause class
Be similar to the aqueous solution the fine liquid stream of polar liquid by becoming very difficult, its flow resistance is big, or even general Micropump is all
It is difficult to promote, certainly, if cover plate also selects to use the PDMS material, then, problem is substantially identical, similar；
Therefore, among prior art, modification is modified particular for the micro-channel inner surface on the PDMS material, is necessary
Operation；So, this is pretty troublesome for the modified operation of PDMS micro-channel inner surfacesThat falls nor this problem, structure
Perplex into serious technical, be another problem：PDMS polymer molecules tool inside its body phase of this PDMS material substrate
There are PDMS polymer molecules inside the characteristic for diffusing to the surface, migrating automatically, this substrate body phase to diffuse to the surface, move automatically
The characteristic of shifting, will cause the state after modification by its inner surface of that micro-channel of surface modifying and decorating can not maintain foot
Enough long time, the holding time for micro-channel its inner surface state after that is surface-modified is substantially only sufficient to complete in laboratory
The time of portion's test experiments needs；In other words, by surface modification or the PDMS micro-channel inner surfaces of surface modification, its
The surface state that is formed can not be lasting after modification or after saying modification, but soon automatically tend to or say and become surface again and change
Surface state before property, returns to the strong hydrophobic surface state of that script in the shorter time, then, just think,
Such micro-fluidic chip can largely make, mass storage, be widely popularized, and answer is it is obvious that is, impossible.
Micro-channel on this PDMS material, can not pump similar to the fine liquid stream of polar solvent of the aqueous solution if not doing surface modification
Send and pass through, chip also cannot just be used；And if having done surface modification, the state after its modification can not be persistently kept again, also
Being equally can not popularization and application.
So, how to accomplish that substrate can either be made using cheap PDMS material, and table in the micro-channel can be released
Face decorating state can not persistently, chip can not largely make, largely lay in so that be widely popularized it is such a make this area it is numerous specially
The puzzlement that industry personnel are entangled with for a long time, the highly difficult problem that exactly one its obvious technology barrier can not despise.
Be present many year in the highly difficult problem, so far, not yet properly settled.
Second, the PDMS material of non-surface modification, above it is stated that, its surface is strongly hydrophobic, this strong hydrophobic
Material surface and also another problem, that is, this strong hydrophobic PDMS surfaces can adsorb large biological molecule, and
And, these adsorbed large biological molecules can also further depression further on PDMS surfaces, gradually fall into gradually deep, directly
It is trapped in heavy within the body phase of PDMS substrates, in fact, this process is partly also due to inside PDMS material body phase
Polymer molecule, which has, to be diffused to the surface, caused by travel motion；Such case, can also be explained from another angle, i.e.
Continuously from inside PDMS body phases to its diffusion into the surface, migration those polymer molecules, its result moved, be by
Gradually those are involved within the body phase of PDMS substrates by the large biological molecule of adsorption, briefly, these are inhaled
Attached large biological molecule is exactly to be swallowed up by PDMS substrate body phases；So, this PDMS substrates body phase swallows up large biological molecule
Phenomenon, the influence caused by it necessarily causes the severe deviations of all kinds of test data of experiment for being related to large biological molecule.
As described above, be the problem of PDMS substrates, its not only adsorption large biological molecule, and swallow up large biological molecule,
So, as the large biological molecule of experiment test object, its disappearance will not stop because the absorption of surface saturation, but, no
It is disconnected adsorbed, also constantly swallowed up.
On PDMS substrates in related experiment test process its body phase constantly swallow up test associated biomolecule macromolecular phenomenon, separately
It is to say that one kind, which is explained, there are substantial amounts of Minute pores in PDMS body phases, and associated biomolecule macromolecular is by after adsorption, depression
Into these Minute pores, and then swallowed up；However, inventor thinks, those can allow the air point of miniature scale
Son squeezes into the Minute pores therebetween, and being not equal to them also can directly allow that the large biological molecule of relative large scale enters, and two
Person's difference on yardstick is huge, must not make sweeping generalizations.Explanation is bypassed, in any case, the biology of object is analyzed as dependence test
Macromolecular is adsorbed by PDMS substrate micro-channels inner surface, and then is constantly swallowed up by PDMS substrate body phases, and this is known objective
The phenomenon of presence.
, can be from containment PDMS surfaces to life in order to prevent this PDMS substrate bodies relative to the effect of swallowing up of large biological molecule
The absorption of thing macromolecular is addressed, and method is chemically modified modification aiming at the PDMS material surface, for
For PDMS is the situation of substrate material, modification exactly is chemically modified to the surface of described micro-channel part, by changing
The micro-channel inner surface of modification is learned, its absorption to large biological molecule can be contained, and then avoid large biological molecule quilt
PDMS substrate body phases are swallowed up；But, or that old problem, that is, the chemical modification on PDMS material surface changes
Surface state after property can not persistently be kept, the polymer molecule inside the PDMS substrate body phases its diffuse to the surface automatically,
The process of migration, soon can become that micro-channel inner surface state being modified by surface chemical modification again script strong and dredge
Water and the state of strong adsorption large biological molecule, in other words, no matter how professionals in the field turn from side to side, the PDMS
Its micro-channel inner surface of substrate is always rapidly to strong hydrophobic surface state evolution.
So, how can either obtain that PDMS material price is extremely cheap, substrate makes extremely easy benefit, can reach again
Growth stage contains absorption process of the PDMS substrate micro-channel inner surfaces to large biological molecule, and then prevents PDMS substrate body phases
The effect of swallowing up to large biological molecule so that related chip manufactured goods are able to maintain that a prolonged enough, rational shelf-life,
It is exactly a very intractable problem.The problem makes the numerous specialties in this area as another problem addressed above, equally
Personnel are entangled with, perplexed for a long time, and the problem is equally the highly difficult problem that its obvious technology barrier can not despise.The hardly possible
Also be present many year in topic, so far, also not yet properly settled.
The content of the invention
The technical problems to be solved by the invention are to provide a package solution, solve what is addressed above totally
Two problems, also, by the solution be applied to build it is a kind of it is new can be for six kinds than more typical primary tumor mark
Will thing carries out examination simultaneously, while the micro flow control chip device of detection.
The present invention solves the technical problem by following scheme, the device that the program is provided be it is a kind of dismount it is convenient to both brief
Classifiable tumor mark joint inspection chip apparatus, the program is characterized in that the structure of the device includes multichannel micro-fluidic chip,
The structure of the micro-fluidic chip includes installing substrate and cover plate together bonded to each other, the substrate and cover plate be plate object or
Contain the channel structure via mould pressing process or etching technics formation, the base in tablet, that face towards the cover plate of the substrate
Piece also contains and is connected and pierces being formed via mould pressing process, etching technics or simple drilling technology for the substrate with the channel structure
Window structure, substrate bonded to each other being installed together and the cover plate have been built into containing pipeline configuration and phase therewith jointly
The micro-fluidic chip of liquid pool structure even, the locations of structures of the pipeline is located at the substrate and cover plate interface zone bonded to each other,
Its side of the window is blocked by the cover plate and opposite side is opened, and the locations of structures of the window is exactly the locations of structures of the liquid pool, institute
Stating liquid pool has two kinds, and two kinds of liquid pools are the sample introduction end liquid pool and terminal liquid pool positioned at different structure position respectively, and this is micro-fluidic
The sample introduction end position of chip has one or more sample introduction end liquid pool, and the sample introduction end refers to that the micro-fluidic chip is real
The injection end position of detected solution during the sample introduction of border, then has a terminal liquid pool in the terminal location of the micro-fluidic chip, described
Terminal refers to the terminal location of liquid flowing in its chip when the actual sample introduction of the micro-fluidic chip is tested, the terminal and the sample introduction end
It is located remotely from each other, one end of the pipeline and the sample introduction end liquid pool UNICOM positioned at sample introduction end, the other end of the pipeline are micro- with being located at this
The terminal liquid pool UNICOM of the terminal of fluidic chip, and, sequentially or backward be respectively installed in the pipeline it is different
Working electrode on position and to electrode and reference electrode, the order refers to that its locations of structures of the reference electrode is more leaned on
The nearly terminal location, the backward refers to the reference electrode locations of structures closer to the sample introduction end position, the work
Electrode is made up of conductive electrode and the gold size sensitive membrane for having embedded tumor markers antibody being attached on the conductive electrode,
Parallel construction is presented in the construction of the pipeline, and the pipeline in parallel construction is made up of six lateral parallel connections, and the presentation is simultaneously
Its appearance profile of the pipeline of connection construction is similar to the profile of parallel circuit, and the quantity of the working electrode is six, this six
The installation position of working electrode is located in six laterals respectively, and, its top layer gold size of six working electrodes is sensitive
Tumor markers antibody in membrane structure is the six kinds of tumor markers antibody that can be specifically bound to tumor markers antigen respectively
Material, six kinds of antibody materials are tumor markers antibody A FP, CEA, PSA, CA125, CA19-9 and CA15-3 respectively,
The antigen is the antigen of broad sense, and the antibody is the antibody of broad sense, and its material of the working electrode is argent material, gold
Column, sheet or thread is presented in material, carbon material or thermal decomposition conducting polymer material, its pattern of the working electrode, should
Its material of substrate is dimethyl silicone polymer material, and its surface of the substrate is the surface of primary form, the surface of the primary form its
It is intended to refer to not by any surface chemical modification or the surface of the primary form of the material of any surface chemical modification, should
The structure of device also includes hinge-type fixture, hinge-type fixture its appearance profile likeness in form hinge, and the hinge-type fixture is by mutually cutting with scissors
Two hinges being connected together and matched through a fastening screw of two hinges and one with the fastening screw and with
The nut for being used for fastening and hand break loose manually that the fastening screw connects together is constituted, and two hinges of the hinge-type fixture are each
Mutually drawn close with its tip and clamp the micro-fluidic chip, the tip is positioned at the knot of the neighbouring terminal of the micro-fluidic chip
Fixation, which is attached, on structure position, in a hinge at least in is equiped with miniature ultrasonic transducer units, and, high frequency vibrating
Electric signal transmission cable is swung, one end and the miniature ultrasonic transducer units of the higher-order of oscillation electric signal transmission cable link together；
The hinge-type fixture facilitates the function that the device is disassembled there is provided one；Its major function of the miniature ultrasonic transducer units is in miniflow
When controlling the actual sample introduction of chip and testing, the ultrasonic wave launched using it reduces the boundary between sample solution and the inwall of the pipeline
Face tension force, can be compatible, also, installs position with the miniature ultrasonic transducer units using the sample introduction end and the terminal
The distance between put the difference on difference and its ultrasonic intensity experienced, its interfacial tension of sample introduction end described in induced synthesis
With the difference between the terminal its interfacial tension, the interfacial tension difference between the micro-fluidic chip two ends can be micro-fluidic at this
Pressure gap is formed between the two ends of chip, the pressure gap can drive sample solution to the end flow；The miniature ultrasonic
Wave transducer its function also include with its ultrasonic wave launched check large biological molecule contained in sample its in the pipeline
Absorption on inner surface, and then check swallow up work of its body phase of the substrate of the dimethyl silicone polymer material to the large biological molecule
With；The substrate of the dimethyl silicone polymer material its function include with cover plate and working electrode and to electrode and reference electrode together
Build the micro-fluidic chip, it is soft and have the substrate of the dimethyl silicone polymer material of elasticity its function and also include with it to ultrasound
The property that ripple absorbs strongly, is absorbed strongly to ultrasonic wave, and thereby in the micro-fluidic chip terminal between the sample introduction end
Limited short distance within realize the rapid decrement of ultrasonic intensity；And, ultrasonic wave damper, the ultrasonic wave damper its
Profile is in block, tabular or bar-shaped；Its material of the ultrasonic wave damper is microcellular rubber, sponge rubber or microporous polyurethane elasticity
Body；And, elastic clip, the elastic force accompanies two clamping limbs, the medial surface of each clamping limb and the medial surface of another clamping limb
Relatively, the medial surface of one of clamping limb is close to the sample introduction end of the micro-fluidic chip, and the medial surface of another clamping limb is close to
The ultrasonic wave damper, the elastic clip is mutual by the sample introduction end of the micro-fluidic chip and the ultrasonic wave damper with its gripping strength
Snugly clip together, the strong consumption to ultrasonic wave that the ultrasonic wave damper is brought with its material, further support
The rapid decrement of ultrasonic intensity is realized within micro-fluidic chip terminal to the limited short distance between the sample introduction end, and
Thereby further expand the difference between described its interfacial tension of sample introduction end and the terminal its interfacial tension.
The tumor markers antibody is the antibody of broad sense, and the antibody of the broad sense refers to possessing antibody function or functionally similar
Can occur to combine and formed immune complex or immune compound with the various involved tumor markerses of corresponding clinic in antibody
The material of the analog of thing；The tumor markers antigen is the antigen of broad sense, and the antigen of the broad sense is referred to can be using accordingly
Antibody or be functionally similar to antibody material carry out enzyme mark detection it is various it is clinical involved the need for differentiate, the tumor-marker of detection
Thing.
Described miniature ultrasonic transducer units can certainly be all installed in two hinges；But, only installing one is micro-
Type ultrasonic transducer is dealt with enough to be used.
The word of hinge one art-recognized meanings of itself are known.
The gold size sensitive membrane is to be sufficiently mixed chitosan gold size solution and tumor markers antibody-solutions uniformly, uses point sample instrument
Point sample is coated on specified structure position, and forms its drying and forming-film.Tumor markers antibody in the gold size sensitive membrane is equal
The tumor markers antibody marked for horseradish peroxidase or glucose oxidase, the gold size sensitive membrane has been included as fixing
Above-mentioned each tumor markers antibody and introduce complementary medium therein, the complementary medium for example chitosan, cellulose acetate,
Gelatin is therein a kind of or their mixture.
The pipeline in the microfluidic chip structure includes the lateral, and its internal diameter size may each be arbitrarily selected
Size, still, for less with the consideration in terms of prepare liquid sample and reduction reagent loss, the pipeline includes described as far as possible
The passage of the preferred capillary level of lateral, the passage of the capillary level implies that the interior of its internal diameter and the capillary on ordinary meaning
The suitable passage in footpath.The shape of cross section of its inner passage of capillary can be arbitrary shape, the shape of cross section example
Such as circular, oval, square, rectangle, bar shaped, naturally it is also possible to be arbitrarily the presence of the linear of bending, also, the hair
The interior shape of tubule is with the extension of pipeline, and the shape of cross section of different parts can also allow to be different shapes.Only with regard to hair
For the word of tubule one, its art-recognized meanings is known.
What is be related in structure is the electrode of microsize to electrode and reference electrode, and its electrode shape may each be any choosing
Fixed shape, the arbitrarily selected shape such as column, sheet, strip or thread etc..It is described to electrode and the ginseng
Art-recognized meanings than the electrode vocabulary of itself are known.
It is related to several liquid pools in this case microfluidic chip structure, the liquid pool is the pond shape or scrotiform structure for transitional liquid storage
Make, its shape of the inner chamber of each liquid pool may each be arbitrarily selected shape, the cavity shape such as cylindrical empty
Cavity-like, square column type cavity-like, oblong cavity shape or spherical hollow space shape etc..
It is public only for professional of the word of ultrasonic transducer one art-recognized meanings of itself for ultrasonic technology field
Know.
Various sizes, variously-shaped ultrasonic transducer are commercially available；Commercially available miniature ultrasonic transducer units its sizes can be with
It is small to the magnitude only calculated with millimeter.
Only with regard to miniature ultrasonic transducer units its technique for fixing on general industry application solid body surface itself and
Speech, is known general technology for the professional in ultrasonic technology field.This case is not to this expansion superfluous words.
Only with regard to naked PDMS substrates itself micro-channel molding or lithographic technique for, be open-and-shut known technology；Together
Sample, the technology of hole-opening is even more known simple technique on naked PDMS substrates.This case is not also to this expansion superfluous words.
The industrial products market of involved its all size of higher-order of oscillation electric signal transmission cable is on sale.
The structure of the device can also include higher-order of oscillation electric signal generator；Its is another for the higher-order of oscillation electric signal transmission cable
One end can be connected with the higher-order of oscillation electric signal generator.
The involved higher-order of oscillation electric signal generator technology of itself, for the professional in ultrasonic technology field, be
It is simple and known；The higher-order of oscillation electric signal generator can be customized to ultrasonic instrument specialized factory.
The preferred scope of its specified ultrasonic wave transmission power of the miniature ultrasonic transducer units be between 5 milliwatts and 9000 milliwatts it
Between；The preferred scope of the frequency of its ultrasonic wave operationally launched of the miniature ultrasonic transducer units be between 100KHz with
Between 12MHz.
This case device can further include some annexes, the annex such as multiple tracks electrochemical workstation etc., institute certainly
The art-recognized meanings for stating multiple tracks electrochemical workstation are known.Each working electrode for being related in this case microfluidic chip structure and
, can be respectively via corresponding special the corresponding interface got lines crossed with the multiple tracks electrochemical workstation to electrode and reference electrode etc.
Coupled.It is described that special to get lines crossed be for each the corresponding interface of each electrode and the multiple tracks electrochemical workstation is carried out into phase
The private cable being mutually coupled with.The micro-fluidic chip in this case device, its structure can also include micro-valve, the number of the micro-valve
Amount is not limited, according to actual needs, and the micro-valve can be installed in any required position installed in the microfluidic chip structure；
The word of micro-valve one is for the professional of micro fluidic chip technical field, and the art-recognized meanings of itself are known；The micro-valve
The manufacturing technology of itself and the use of technology is also known；The component that the micro-valve is not required.
The diameter of the working electrode can allow to be that any setting is easily installed the suitable diameter used, it is however recommended to
Or say preferred its scope of the diameter between 0.1 micron to 2000 microns；The length of the working electrode can permit
Permitted to be that any setting is easily installed the length used, it is however recommended to or to say preferred its scope of the length be at 1 micron
To between 15000 microns.
The gold size for being installed in the working electrode surface layer by spraying or point sample instrument point sample or the coating of other appropriate process is quick
Feel film, its thicknesses of layers can allow be any setting treat sample measuring liquid occur electrical signals response thickness, still, push away
Preferred thickness is between 10 nanometers and 200 nanometers in other words for the thickness recommended.
The cover plate in chip structure, its material can allow to be any electrical insulating property material, for example：Polypropylene, glass
Glass, polymethyl methacrylate, dimethyl silicone polymer, etc., in order to make smaller size of micro-fluidic chip, such as do
Realized into the micro-fluidic chip of only 2.0 centimetres to 3.0 centimetres of super-small of length, and in the extremely short distance to ultrasonic wave
Extremely fast decay, can preferably dimethyl silicone polymer be used as cover plate.Certainly, selected on large-sized micro-fluidic chip
It is used as the cover plate using dimethyl silicone polymer, is also that this case technical scheme is allowed.
The preferred scope of the distance between the terminal and the sample introduction end is between 3 centimetres and 10 centimetres.
Described its thickness of cover plate and substrate can allow be any setting be easy to assembling thickness, the thickness of recommendation or say preferably
Thickness be between 1.0 millimeters and 5.0 millimeters.Less thickness is conducive to saving material.
Described its size of ultrasonic wave damper is not limited, and described its length of ultrasonic wave damper, width, thickness may each be basis
Need the arbitrary size of setting.
It is known in field of rubber technology only for the word of microcellular rubber one art-recognized meanings of itself.
The microcellular rubber is commercially available.
It is known in field of rubber technology only for the word of sponge rubber one art-recognized meanings of itself.
The sponge rubber is commercially available.
It is known in technical field of macromolecules only for the word of microporous polyurethane elastomer one art-recognized meanings of itself.
The polyurethane micropore elastomer material is commercially available.
The microcellular rubber preferred microporous silicon rubber or micropore fluorubber.
It is known in field of rubber technology only for the word of micropore silicon rubber one art-recognized meanings of itself.
The microporous silicon Rubber market is on sale.
It is known in field of rubber technology only for the word of micropore fluorubber one art-recognized meanings of itself.
The micropore fluorubber is commercially available.
The word of elastic clip one art-recognized meanings of itself are known.
The elastic clip is commercially available.
The application method of this case micro-fluidic chip：
Proposed first based on this case and the first public New stream driving principle, its application running of this case micro-fluidic chip
In, the New stream driving method is determined completely without involving any additional Micropump.
The interfacial tension difference of this case to be formed between micro-fluidic chip two ends caused by the ultrasonic wave, drives liquid
Stream flows in the capillary channel of the six-channel microfluidic chip, using multi-channel electrochemical analyzer device respectively to six kinds of typical cases
Tumor markers antigen carries out joint-detection.
The specific detection of this case micro-fluidic chip is as follows using step：
1st, blood serum sample liquid is added in micro-pipe road, under ultrasonic wave driving, various tumor markers antigen molecules are each
In passage on electrode surface gold size sensitive membrane embed corresponding horseradish peroxidase-labeled tumor markers antibody capture.
2nd, the tumor markers antigen in the tumor markers antibody and blood serum sample of horseradish peroxidase-labeled forms immune multiple
Compound.
3rd, using multi-channel electrochemical analyzer, the electron mediators such as catechol are added, using the above-mentioned reaction of amperometric detection
Caused curent change, is derived from the species and content of various analytes.
4th, result is subjected to comprehensive analysis, comprehensive diagnos is carried out to tumor markers antigen.
It is an advantage of the invention that its close position positions the hinge-type fixture in the terminal of the micro-fluidic chip, with
The miniature ultrasonic transducer units installed, the low-power launched using it, high-frequency band are attached in its hinge of the hinge-type fixture
Ultrasonic wave so that without surface chemical modification or surface chemical modification strong hydrophobic micro-fluidic chip internal pipeline its
Compatibility between tube wall and the test object aqueous solution is significantly increased, and this passes through possible there is provided a reality for test liquid stream
Property；Meanwhile, using its strong absorbability to ultrasonic wave of dimethyl silicone polymer substrate, in shorter distance, also
Be, from the terminal to the very short distance of only several centimeters yardsticks the sample introduction end in, reach the quick of ultrasonic intensity
Successively decrease, the difference of the interfacial tension is thereby caused at the two ends of the micro-fluidic chip, and then, using between the two ends
Pressure gap between its two ends for being formed of the difference of interfacial tension, driving test liquid stream is strongly hydrophobic in such a script
Capillary channel in the terminal direction flow.By this case liquid stream drive scheme, entirely without must be to the polydimethylsiloxanes
The substrate and its internal pipeline of alkane material carry out any surface chemical modification or chemical modification, have altogether dispensed with the surface chemistry and have repaiied
The laborious procedures of decorations or chemical modification；And altogether dispense with the equipment of traditional Micropump etc；On the other hand, this is low
The ultrasonic wave of power, high-frequency band, additionally it is possible to contain the large biological molecule in sample in the literalness naked polydimethylsiloxanes
Absorption on its inner surface of pipeline of alkyl piece, and then contain its body phase of the dimethyl silicone polymer substrate to the large biological molecule
The effect of swallowing up；The Reversible binding thing of the antigen, antibody and antigen and antibody is all to belong to described large biological molecule certainly
Type；Because described suction-operated and the described effect of swallowing up effectively are contained that therefore, dependence test result will more
Actual conditions can objectively be reflected；The effect of the low-power, high-frequency band ultrasonic wave, also includes facilitating antigen, antibody certainly
Between Reversible binding reaction quick reaching, this causes dependence test operation can be to be completed than faster speed.
Due to the surface chemical modification for the dimethyl silicone polymer substrate its relevant surfaces or chemical modification behaviour need not be carried out
Make, therefore, this surface chemical modification layer or chemically modified layer not need to exist, then, the dimethyl silicone polymer
Its body phase interior polymer molecule of substrate constantly diffuses to the surface automatically, migrate caused by it to surface chemical modification layer or
The damaging influence of chemically modified layer is also just not present.
This case its installation position is located at the ultrasonic wave damper at the sample introduction end, from its terminal of this case micro-fluidic chip to
Said on the sport technique segment that ultrasonic intensity rapid decrement is realized in the such short distance in sample introduction end, its effect of the ultrasonic wave damper is suitable
In the size expansion device of micro-fluidic chip, in other words, the ultrasonic wave damper its function as and significantly extend miniflow
Its terminal of chip is controlled the distance between to sample introduction end, is the equal of from several centimetres of extension of script to more than ten centimetres of length
Or twenties centimetres of length or longer length, so so that described terminal to the ultrasonic wave between sample introduction end in short distance
The sport technique segment of intensity rapid decrement can relatively easily be reached, also, the size expansion device namely the ultrasonic wave damper
Its installation and removal is all very convenient, it is important that it can also be reused.
The technical scheme of this case has dissolved its application related one to dimethyl silicone polymer substrate addressed above totally
Row technical barrier.Based on this case scheme, this kind very cheap polydimethyl siloxane material is just possible in the micro-fluidic chip
Prepare, production, using etc. field play bigger effect.
The miniature ultrasonic transducer units have been installed in fixation in the hinge-type fixture its hinge in this case structure, and the structure is provided
One facilitates the function that the device is disassembled, in this way, the hinge-type fixture is together with appended miniature ultrasonic transducer units in its hinge
Just easily it can be mutually disengaged with the micro-fluidic chip, then, the component that the part can freely depart from just can circulate benignly ground
Reuse many times；The architectural feature is conducive to saving the use cost of the device.
The framework of its large-scale integrated of this case micro-fluidic chip, determines it among actual test application, to Serum samples
The need for measure smaller, this helps to reduce the body and mind damage of related subject.
Brief description of the drawings
Fig. 1 is its rough outside side view of this case device.
In figure, 1 is hinge-type fixture, and 2 indicate linkwork positions, and 3 be fastening screw, and 4 be higher-order of oscillation telecommunication
Number transmission cable, 5 be miniature ultrasonic transducer units, and 6,11 be two different hinges of locations of structures respectively, and 7 be cover plate, 8
It is substrate, 9 be sample introduction end, and 10 be terminal, and 12 be nut；The hinge-type clamp structure in legend is only the legend of signal
Structure, actual its structure of the hinge-type fixture is not limited to the legend hinge-type clamp structure；It is micro- that arrow in legend indicates this
Fluidic chip its in actual motion, by pressure at two ends difference drive, the flow direction of its test liquid stream.
It is depicted without being also depicted without the elastic clip in the ultrasonic wave damper, legend in legend.
Embodiment
In this case that Fig. 1 is shown embodiment, its main points of the example are that the structure of the device includes multichannel micro-fluidic core
Piece, the structure of the micro-fluidic chip includes the substrate 8 and cover plate 7 of installing bonded to each other together, the substrate 8 and cover plate 7
It is plate object or tablet, that face towards the cover plate 7 of the substrate 8 is contained to be formed via mould pressing process or etching technics
Channel structure, the substrate 8 also containing be connected with the channel structure and pierce the substrate 8 via mould pressing process, etch work
Skill or the window structure of simple drilling technology formation, substrate 8 bonded to each other being installed together are built into jointly with the cover plate 7
Micro-fluidic chip containing pipeline configuration and the liquid pool structure being attached thereto, the locations of structures of the pipeline be located at the substrate 8 with
The cover plate 7 interface zone bonded to each other, its side of the window is blocked by the cover plate 7 and opposite side is opened, the structure of the window
Position is exactly the locations of structures of the liquid pool, and the liquid pool has two kinds, and two kinds of liquid pools are entering positioned at different structure position respectively
There is the one or more sample introduction end liquid sample end liquid pool and terminal liquid pool, the position of sample introduction end 9 of the micro-fluidic chip
Pond, the sample introduction end 9 refers to the injection end position of detected solution during the micro-fluidic chip actual sample introduction, in the micro-fluidic chip
The position of terminal 10 then have a terminal liquid pool, the terminal 10 refers to during the actual sample introduction test of the micro-fluidic chip it
The terminal location that liquid flows in chip, the terminal 10 is located remotely from each other with the sample introduction end 9, and one end of the pipeline is with being located at sample introduction
The sample introduction end liquid pool UNICOM at end 9, the other end of the pipeline and the end of the terminal 10 positioned at the micro-fluidic chip
Hold liquid pool UNICOM, and, sequentially or backward be respectively installed in working electrode in the pipeline on diverse location and to electrode
And reference electrode, the order refers to its locations of structures of the reference electrode closer to the position of terminal 10, described inverse
Sequence refers to the reference electrode locations of structures closer to the position of sample introduction end 9, the working electrode by conductive electrode and
The gold size sensitive membrane for having embedded tumor markers antibody being attached on the conductive electrode is constituted, and the construction of the pipeline is presented in parallel
Construction, the pipeline in parallel construction is made up of six lateral parallel connections, and the pipeline of the presentation parallel construction is outside it
Shape profile is similar to the profile of parallel circuit, and the quantity of the working electrode is six, the installation position point of six working electrodes
Not Wei Yu in six laterals, and, the tumor markers in the sensitive membrane structure of its top layer gold size of six working electrodes
Antibody is the six kinds of tumor markers antibody materials that can be specifically bound to tumor markers antigen, six kinds of antibody materials point respectively
It is not tumor markers antibody A FP, CEA, PSA, CA125, CA19-9 and CA15-3, the antigen is the antigen of broad sense,
The antibody is the antibody of broad sense, and its material of the working electrode is argent material, gold material, carbon material or thermal decomposition
Column, sheet or thread is presented in conducting polymer material, the working electrode its pattern, and its material of the substrate 8 is poly dimethyl
Siloxanes material, its surface of substrate 8 is the surface of primary form, the surface of the primary form its be intended to refer to not pass through
The surface of the primary form of any surface chemical modification or the material of any surface chemical modification, the structure of the device also includes closing
Page fixture 1, the hinge-type fixture 1 its appearance profile likeness in form hinge, the hinge-type fixture 1 by be mutually hinged two
Individual hinge 6,11 and a fastening screw 3 through two hinges 6,11 and one match with the fastening screw 3
And the nut 12 for being used for fastening and hand break loose manually connected together with the fastening screw 3 is constituted, the hinge-type fixture 1
Two hinges 6,11 respectively mutually drawn close with its tip and clamp the micro-fluidic chip, the tip is positioned at the micro-fluidic chip
The neighbouring terminal 10 locations of structures on, a hinge at least in for example attaches fixed installing in hinge 6
There are miniature ultrasonic transducer units 5, and, higher-order of oscillation electric signal transmission cable 4, the higher-order of oscillation electric signal transmission cable 4
One end linked together with the miniature ultrasonic transducer units 5；The hinge-type fixture 1 facilitates what the device was disassembled there is provided one
Function；Its major function of the miniature ultrasonic transducer units 5 is, in the actual sample introduction test of micro-fluidic chip, to be launched using it
Ultrasonic wave reduces the interfacial tension between sample solution and the inwall of the pipeline, can be compatible, also, using described
The distance between sample introduction end 9 and the terminal 10 and the installation position of miniature ultrasonic transducer units 5 difference and its experienced
To ultrasonic intensity on difference, its interfacial tension of sample introduction end 9 described in induced synthesis and described its interfacial tension of terminal 10 it
Between difference, interfacial tension difference between the micro-fluidic chip two ends 9,10 can the two ends 9 of the micro-fluidic chip,
Pressure gap is formed between 10, the pressure gap can drive sample solution to be flowed to the terminal 10；The miniature ultrasonic transducing
Device 5 its function also includes checking contained its table in the pipeline of large biological molecule in sample with its ultrasonic wave launched
Absorption on face, and then check swallow up effect of its body phase of the substrate 8 of the dimethyl silicone polymer material to the large biological molecule；
The substrate 8 of the dimethyl silicone polymer material its function include with cover plate 7 and working electrode and to electrode and reference electrode together
Build the micro-fluidic chip, it is soft and have the substrate 8 of the dimethyl silicone polymer material of elasticity its function and also include with it to super
The property that sound wave absorbs strongly, is absorbed strongly to ultrasonic wave, and thereby in the micro-fluidic chip, the terminal 10 arrives the sample introduction
The rapid decrement of ultrasonic intensity is realized within limited short distance between end 9；And, ultrasonic wave damper, the ultrasonic wave
Its profile of damper is in block, tabular or bar-shaped；Its material of the ultrasonic wave damper is microcellular rubber, sponge rubber or polyurethane
Micro-pore elastomer；And, elastic clip, the elastic force accompanies two clamping limbs, the medial surface of each clamping limb and another clamping limb
Medial surface it is relative, the medial surface of one of clamping limb is close to the sample introduction end 9 of the micro-fluidic chip, another clamping limb
Medial surface is close to the ultrasonic wave damper, and the elastic clip is with its gripping strength by the sample introduction end 9 of the micro-fluidic chip and the ultrasound
Ripple damper is clipped together bonded to each otherly, and the strong abatement to ultrasonic wave that the ultrasonic wave damper is brought with its material is made
With further in the micro-fluidic chip, the terminal 10 arrives realization ultrasound within the limited short distance between the sample introduction end 9 for support
The rapid decrement of intensity of wave, and thereby further expand described its interfacial tension of sample introduction end 9 and described its interfacial tension of terminal 10
Between difference.
It is depicted without being also depicted without the elastic clip in the ultrasonic wave damper, legend in legend.
Arrow in legend indicate the micro-fluidic chip its in actual motion, by pressure at two ends difference drive, its test liquid
The flow direction of stream.
The associate members such as the higher-order of oscillation electric signal generator and multiple tracks electrochemical workstation are depicted without in Fig. 1.
Involved hinge-type fixture 1 can be carried out simply cutting using commercially available hinge, changed a social system；The miniature ultrasonic transducing
Device 5 is commercially available；Commercially available miniature ultrasonic transducer units are various in style, can select to use as needed；The miniature ultrasonic
Transducer 5 can also be customized to ultrasonic transducer specialized factory.
Involved higher-order of oscillation electric signal transmission cable 4 is commercially available；Can also be to ultrasonic transducer producer or cable special manufacturer
Family's customization.
Involved higher-order of oscillation electric signal generator in the market has the product close to needs commercially available；It can also be customized to relevant manufacturers.
Each working electrode in this example structure and electrode and reference electrode via each special cable or can be said respectively
Get lines crossed respectively with the corresponding cable interface of the multiple tracks electrochemical workstation as annex or saying interface connection of getting lines crossed.
The multi-channel electrochemical work station is commercially available；The multi-channel electrochemical work station can also be as needed to specialty
Producer customizes.
In view of this case pipeline of the presentation parallel construction its form that related text expresses that it is described above is clear enough
It is clear, the concrete form of the pipeline in this kind of micro-fluidic chip of this case is no longer specifically illustrating in this case embodiment.
Antibody described in this case refers to the antibody of broad sense；Antigen described in this case refers to the antigen of broad sense；It is immunized described in this case multiple
Compound refers to the immune complex of broad sense.
Claims (10)
1. dismounting brief classifiable tumor mark joint inspection chip apparatus convenient to both, it is characterised in that the structure of the device includes
Multichannel micro-fluidic chip, the structure of the micro-fluidic chip includes the substrate and cover plate of installing bonded to each other together, the substrate
It is plate object or tablet with cover plate, that face towards the cover plate of the substrate is contained via mould pressing process or etching technics shape
Into channel structure, the substrate also containing be connected with the channel structure and pierce the substrate via mould pressing process, etching technics
Or the window structure of simple drilling technology formation, substrate bonded to each other being installed together has been built into jointly with the cover plate to be contained
The micro-fluidic chip of pipeline configuration and the liquid pool structure being attached thereto, the locations of structures of the pipeline is located at the substrate and the cover plate phase
The interface zone mutually fitted, its side of the window is blocked by the cover plate and opposite side is opened, and the locations of structures of the window is exactly described
The locations of structures of liquid pool, the liquid pool has two kinds, two kinds of liquid pools respectively be positioned at different structure position sample introduction end liquid pool and
Terminal liquid pool, the sample introduction end position of the micro-fluidic chip has one or more sample introduction end liquid pool, and the sample introduction end refers to
Be the micro-fluidic chip actual sample introduction when detected solution injection end position, then have one in the terminal location of the micro-fluidic chip
The terminal liquid pool, the terminal refers to the terminal position of liquid flowing in its chip when the actual sample introduction of the micro-fluidic chip is tested
Put, the terminal is located remotely from each other with the sample introduction end, one end of the pipeline and the sample introduction end liquid pool UNICOM positioned at sample introduction end, the pipe
The other end in road with positioned at the micro-fluidic chip the terminal the terminal liquid pool UNICOM, and, sequentially or backward point
The working electrode that is not installed in the pipeline on diverse location and to electrode and reference electrode, the order refers to the ginseng
Than its locations of structures of electrode closer to the terminal location, the backward refers to the reference electrode locations of structures closer to described
Sample introduction end position, the working electrode is resisted by conductive electrode and the tumor markers that embedded being attached on the conductive electrode
The gold size sensitive membrane of body is constituted, and parallel construction is presented in the construction of the pipeline, and the pipeline in parallel construction is by six laterals
Parallel connection is constituted, and its appearance profile of the pipeline of the presentation parallel construction is similar to the profile of parallel circuit, the working electrode
Quantity be six, the installation positions of six working electrodes is located in six laterals respectively, and, six works
The tumor markers antibody made in the sensitive membrane structure of its top layer gold size of electrode is that tumor markers antigen can be specifically bound respectively
Six kinds of tumor markers antibody materials, six kinds of antibody materials be respectively tumor markers antibody A FP, CEA, PSA, CA125,
CA19-9 and CA15-3, the antigen is the antigen of broad sense, and the antibody is the antibody of broad sense, its material of the working electrode
It is argent material, gold material, carbon material or thermal decomposition conducting polymer material, post is presented in its pattern of the working electrode
Shape, sheet or thread, its material of the substrate are dimethyl silicone polymer materials, and its surface of the substrate is the surface of primary form,
The surface of the primary form its be intended to refer to the material not no by any surface chemical modification or any surface chemical modification
Primary form surface, the structure of the device also includes hinge-type fixture, hinge-type fixture its appearance profile likeness in form hinge,
The hinge-type fixture is by two hinges being mutually hinged and through a fastening screw of two hinges and one
The nut for being used for fastening and hand break loose manually for matching and being connected together with the fastening screw with the fastening screw is constituted, the conjunction
Two hinges of page fixture are respectively mutually drawn close with its tip and clamp the micro-fluidic chip, and the tip is positioned at the micro-fluidic core
Fixation, which is attached, in the locations of structures of the neighbouring terminal of piece, in a hinge at least in is equiped with miniature ultrasonic
Transducer, and, higher-order of oscillation electric signal transmission cable, one end and the miniature ultrasonic of the higher-order of oscillation electric signal transmission cable
Wave transducer links together；The hinge-type fixture facilitates the function that the device is disassembled there is provided one；The miniature ultrasonic transducing
Its major function of device is that in the test of micro-fluidic chip actual sample introduction, the ultrasonic wave launched using it reduces sample solution and institute
The interfacial tension between the inwall of pipeline is stated, can be compatible, also, it is micro- with this using the sample introduction end and the terminal
Difference in the distance between type ultrasonic transducer installation position difference and its ultrasonic intensity experienced, induced synthesis
Interface between difference between described its interfacial tension of sample introduction end and the terminal its interfacial tension, the micro-fluidic chip two ends
Tension difference can form pressure gap between the two ends of the micro-fluidic chip, and the pressure gap can drive sample solution to described
End flow；Its function of the miniature ultrasonic transducer units also includes checking biology contained in sample with its ultrasonic wave launched
Its absorption on the inner surface of pipeline of macromolecular, and then check the substrate of the dimethyl silicone polymer material its body phase to the life
The effect of swallowing up of thing macromolecular；Its function of the substrate of the dimethyl silicone polymer material is included with cover plate and working electrode and to electricity
Pole and reference electrode together build the micro-fluidic chip, soft and have the substrate of the dimethyl silicone polymer material of elasticity its function
Also include with its property to the strong absorption of ultrasonic wave, ultrasonic wave is absorbed strongly, and thereby at the micro-fluidic chip end
Hold the rapid decrement that ultrasonic intensity is realized within the limited short distance between the sample introduction end；And, ultrasonic wave damper,
Its profile of ultrasonic wave damper is in block, tabular or bar-shaped；Its material of the ultrasonic wave damper is microcellular rubber, sponge rubber
Or microporous polyurethane elastomer；And, elastic clip, the elastic force accompanies two clamping limbs, the medial surface of each clamping limb with it is another
The medial surface of individual clamping limb is relative, and the medial surface of one of clamping limb is close to the sample introduction end of the micro-fluidic chip, another folder
The medial surface of gripping arm is close to the ultrasonic wave damper, and the elastic clip is with its gripping strength by the sample introduction end of the micro-fluidic chip with being somebody's turn to do
Ultrasonic wave damper is clipped together bonded to each otherly, the strong abatement to ultrasonic wave that the ultrasonic wave damper is brought with its material
Effect, further supports to realize that ultrasonic wave is strong within micro-fluidic chip terminal to the limited short distance between the sample introduction end
The rapid decrement of degree, and thereby further expand the difference between described its interfacial tension of sample introduction end and the terminal its interfacial tension
It is different.
2. dismounting according to claim 1 brief classifiable tumor mark joint inspection chip apparatus convenient to both, its feature exists
In it is capillary channel that the pipeline, which includes the lateral,.
3. dismounting according to claim 1 brief classifiable tumor mark joint inspection chip apparatus convenient to both, its feature exists
In, the thermal decomposition conducting polymer be by polyimides or polyacrylonitrile be heat-treated through anoxybiotic after the conductive material that is formed.
4. dismounting according to claim 1 brief classifiable tumor mark joint inspection chip apparatus convenient to both, its feature exists
In, the width or diameter of the working electrode between 0.1 micron to 2000 microns, and, the length of the working electrode
Degree is between 1 micron to 15000 microns.
5. dismounting according to claim 1 brief classifiable tumor mark joint inspection chip apparatus convenient to both, its feature exists
In the thickness of the gold size sensitive membrane is between 10 nanometers and 200 nanometers.
6. dismounting according to claim 1 brief classifiable tumor mark joint inspection chip apparatus convenient to both, its feature exists
In its material of the cover plate in structure is dimethyl silicone polymer material.
7. dismounting according to claim 1 brief classifiable tumor mark joint inspection chip apparatus convenient to both, its feature exists
In the distance between the terminal and the sample introduction end are between 3 centimetres and 10 centimetres, described its thickness of cover plate and substrate
Between 1.0 millimeters and 5.0 millimeters.
8. dismounting according to claim 1 brief classifiable tumor mark joint inspection chip apparatus convenient to both, its feature exists
In the microcellular rubber is micropore silicon rubber or micropore fluorubber.
9. dismounting according to claim 1 brief classifiable tumor mark joint inspection chip apparatus convenient to both, its feature exists
Also include higher-order of oscillation electric signal generator in the structure of, the micro fluidic device, its is another for the higher-order of oscillation electric signal transmission cable
One end is connected with the higher-order of oscillation electric signal generator.
10. dismounting according to claim 1 brief classifiable tumor mark joint inspection chip apparatus convenient to both, its feature exists
In its specified ultrasonic wave transmission power of the miniature ultrasonic transducer units is between 5 milliwatts and 9000 milliwatts, the miniature ultrasonic
The frequency of its ultrasonic wave operationally launched of wave transducer is between 100KHz and 12MHz.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610189113.XA CN107199057A (en) | 2016-03-18 | 2016-03-18 | Dismounting brief classifiable tumor mark joint inspection chip apparatus convenient to both |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610189113.XA CN107199057A (en) | 2016-03-18 | 2016-03-18 | Dismounting brief classifiable tumor mark joint inspection chip apparatus convenient to both |
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| CN107199057A true CN107199057A (en) | 2017-09-26 |
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| CN201610189113.XA Pending CN107199057A (en) | 2016-03-18 | 2016-03-18 | Dismounting brief classifiable tumor mark joint inspection chip apparatus convenient to both |
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