CN107918031A - A kind of liquid sample guiding device and the detection device containing the guiding device - Google Patents
A kind of liquid sample guiding device and the detection device containing the guiding device Download PDFInfo
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- CN107918031A CN107918031A CN201610887868.7A CN201610887868A CN107918031A CN 107918031 A CN107918031 A CN 107918031A CN 201610887868 A CN201610887868 A CN 201610887868A CN 107918031 A CN107918031 A CN 107918031A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/66—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood sugars, e.g. galactose
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
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Abstract
A kind of detection device the invention discloses liquid sample guiding device and containing the guiding device, the guiding device include cover board and channel plate, and one end is equipped with sample holes on the cover board, and the other end is equipped with gas outlet；The channel plate is equipped with sample cavity, fluid flow paths and recirculation hole, wherein, sample cavity is located at the lower section of the sample holes, and connect recirculation hole by fluid flow paths, recirculation hole is extended with extensional pipeline relative to the distal end of sample cavity in the channel plate, when cover board closed assembly is on channel plate, channel plate limits a ventilation mouth communicated with the external world by gas outlet, the ventilation mouth is located on any point of the extensional pipeline, or the more far-end positioned at the extensional pipeline end relative to the sample cavity.The detection device also contains substrate, detection strip is formed with above-mentioned cover board, channel plate closed assembly, in addition, further including the detecting instrument containing detection strip.
Description
Technical field
The present invention relates to a kind of instant detection device of blood, and in particular, to a kind of blood guiding device and detection strip,
And the detecting system containing the strip.
Background technology
Immediately detection (POCT, point-of-care testing) refers to the rapid clinical detection carried out beside patient,
Clinical examination teacher is usually not necessarily to carry out, but can be completed by nurse, doctor or even patient oneself.This is that one kind exists
Sampling location is analyzed at once, is saved complex process program of the sample in laboratory inspection, is quickly obtained inspection result
A kind of new method.By definition, POCT is to be carried out in sampling location, is quick using portable analysers and matched reagent
Obtain a kind of detection mode of testing result.
In recent years, POCT is widely used, including each section office of hospital, basic hospital, community clinic and family, such as
Vim and vigour, electrolyte balance, the instant detection of Applications of Cardiac Markers are progressively widely used in emergency ward, ICU and operating room；Family expenses blood
Sugared instrument widely uses the most successful example of even more POCT.
Another example is the quick detection of prothrombin time PT/INR, and INR-POCT systems provide one kind for patient
Simple, fast and easy, safe method monitoring INR values, take finger tip blood examination to survey, easily standardization easy to operate, 1 minute or so
Accurate testing result is can obtain, avoids from collection of specimens, be transported to the delay of detection.Existing INR-POCT systems are more
It is made of an electrochemical analyser and disposable detection strip.
The present invention is suitable for POCT detection strips, it cannot be only used for the quick detection of blood, it can also be used to other liquid
Body sample such as urine.Chinese patent CN2788184Y discloses a kind of biological testing bar, it is related to blood ketone body in detection whole blood
A kind of biological testing bar of concentration or concentration of glucose, it includes：The substrate of electrode is loaded with, is provided with the reaction of reaction reagent
Area space, hydrophilic cover, the wall between the substrate and hydrophilic cover；Wherein, the electrode includes working electrode and ginseng
Compare electrode；There is the groove of openings at one side on the wall；The reaction zone space by have the fluted wall with
The substrate, hydrophilic cover limit to be formed, and have sample holes and exhausting-gas hole.The exhausting-gas hole is located at the hydrophilic cover
On.The utility model for clinically provide it is a kind of can quickly detect the biological testing bar that blood ketone body concentration increases, not only into
This is low, simple in structure and easy to operate, can carry out the quick detection of ketoacidosis.The structure of the biological testing bar
For the usual way of existing coherent detection strip.However, since cover board gas outlet is arranged on the surface of reaction chamber, cause sample
Turbulent flow and bubble are also easy to produce when being flowed to from sample cavity during reaction chamber, so as to influence the repeatability of testing result.
The content of the invention
It is an object of the present invention to providing a kind of liquid sample guiding device, it has more preferable under atmospheric environment
Liquid guide flow function.Further, there is provided a kind of liquid sample detection strip comprising the liquid sample guiding device.More into one
Step ground, there is provided a kind of detecting instrument for include aforesaid liquid pattern detection strip so that carry out POCT detections when reliability with
Repeatability is improved.
To solve above-mentioned first purpose, the technical solution adopted in the present invention is as follows：
A kind of liquid sample guiding device, including cover board and the overlappable channel plate below cover board, one on the cover board
End is equipped with sample holes, and the other end is equipped with gas outlet；The channel plate is equipped with sample cavity, fluid flow paths and recirculation hole, wherein,
When the cover board closed assembly is on the channel plate, the sample cavity is located at the lower section of the sample holes, and passes through the liquid
Body circulation passage connects the recirculation hole, and the recirculation hole of the channel plate is also associated with extensional pipeline, its extending direction is
Relative to the other side of the sample cavity and the liquid communication pipeline, when the cover board closed assembly is on channel plate, institute
State channel plate and one ventilation mouth communicated with the external world is limited by gas outlet, the ventilation mouth is located at appointing for the extensional pipeline
Anticipate on a bit, or the ventilation mouth is at least partly contacted with the extensional pipeline.
It should be noted that liquid sample can flow into sample cavity from sample holes, then liquid passes through fluid flow paths
It flow to recirculation hole.Channel plate limits a ventilation mouth communicated with the external world by the gas outlet of cover board one end, for liquid flow
Liquid in circulation passage is breathed freely with the external world.The ventilation mouth can be located on the optional position of extensional pipeline, include the end of extensional pipeline
End, or ventilation mouth are at least partly contacted with extensional pipeline.It is logical in extension as one of which preferred embodiment, ventilation mouth
The end in road.
Preferably, ventilation mouth is not overlapped with the recirculation hole.
It should be noted that ventilation mouth is located in above-mentioned extensional pipeline any position (rather than located immediately at conversion zone
On), it is that a distance (such as 1mm -10mm) is formed between ventilation mouth and the conversion zone in particular, may be such that liquid energy
More rapidly, uniformly, reliably full of conversion zone.
Preferably, a diameter of 0.1mm -5mm of the gas outlet.
Preferably, the cross-sectional area of the extensional pipeline is relative to the cross-sectional area ratio of the fluid flow paths
1:1–1:10。
Preferably, the length ratio of the length of the extensional pipeline and the fluid flow paths is 1:1–1:10.
Preferably, the tube wall of the fluid flow paths at least partially water wetted material, and the water wetted material position
Through the both ends of fluid connection passage, liquid is thus more advantageous to after sample holes contact guiding device, is led to along fluid connection
Road flows to recirculation hole and therewith into the conversion zone on the substrate of lower section.
It should be noted that by the way that the liquid communication pipeline is at least partly made with water wetted material, band is enabled to
The molecular line liquid sample of polarized group is flowed toward liquid communication pipe end direction；Preferably implement as one of which
Mode, at least wherein one whole section of the tube wall from the liquid communication pipeline head end to end is water wetted material, may be such that liquid
Sample is preferably flowed from sample cavity toward recirculation hole direction.As a more preferred embodiment, the liquid flow siphunculus
The all water wetted materials of tube wall in road, liquid flowing is better under this embodiment.
Preferably, the tube wall of the extensional pipeline at least partially hydrophobic material；It is thin to be preferably close to air outlet parts
Water material, and be water wetted material close to circulation bore portion, the length of water wetted material part is preferably formed with 1-2mm..
It should be noted that the tube wall of the extensional pipeline is made with hydrophobic material at least in part, can prevent
Liquid sample is lost in caused by liquid sample flows into extensional pipeline；As one of which preferred embodiment, prolong described in
At least wherein one whole section of tube wall for stretching pipeline head end to end is hydrophobic material, can preferably prevent liquid sample from liquid flow
Thread a pipe and flowed toward extensional pipeline direction；As a more preferred embodiment, the tube wall of the extensional pipeline is all
Hydrophobic material, prevents liquid from flowing better under this embodiment.
Preferably, the sample cavity, fluid flow paths, recirculation hole, extensional pipeline and ventilation mouth are respectively positioned on same straight line
On.
Wherein, when the sample cavity, fluid flow paths, recirculation hole, extensional pipeline and ventilation mouth are respectively positioned on same straight line
When upper, on the one hand, liquid sample more can be flowed into promptly in recirculation hole from sample cavity, improve flow efficiency；On the other hand, flow
Through hole forms straight-through ventilative with ventilation mouth, it is possible to increase air permeability effect, is that the flowing of liquid improves efficiency.
Preferably, the sample cavity is located at the underface of the sample holes.
It should be noted that when sample cavity is located at the underface of the sample holes, liquid can be unhinderedly from sample holes
Flow into sample cavity.
Preferably, it is characterised in that distance of the ventilation mouth away from the conversion zone end is 1mm -10mm.
Preferably, the area of the ventilation mouth and the area ratio of the sample holes are 1:1–1:100.
Preferably, a diameter of 0.3mm -5mm of described ground ventilation mouth.
To realize above-mentioned second purpose, the technical solution adopted in the present invention is as follows：
A kind of liquid sample detects strip, and containing above-mentioned guiding device, the strip also contains substrate, on the substrate
Equipped with conversion zone, when the substrate closed assembly is under the channel plate, the recirculation hole is located on the conversion zone
It is and least partially overlapped with it.
Wherein, the recirculation hole is used to accept liquid sample so that liquid sample enters reaction zone by the recirculation hole
Domain, the conversion zone provide reaction compartment for liquid sample.
It should be noted that the mounting means as one of which liquid sample detection strip, substrate, channel plate and cover board
Closed assembly successively from the bottom to top.
Preferably, the fluid flow paths are groove, and the groove can be with right and wrong hollow out or hollow out, when described
Groove for hollow out when, when the upper and lower surface difference closed assembly cover board and substrate of channel plate, limited jointly by them Lai
Space forms circulation duct, and the one side wall of pipeline is cover board lower surface, and another side wall is upper surface of base plate.
It should be noted that fluid flow paths have a variety of enforceable modes.For the technical program, liquid communication
When passage can distinguish closed assembly cover board and substrate by the upper and lower surface of groove or channel plate, limited jointly by them Lai
The pipeline that space is formed is realized.As one of which embodiment, the lower section of the fluid flow paths part on channel plate is simultaneously
Not hollow out, but concave liquid flow straight slot downwards is formed, liquid is flowed into conversion zone by groove；Implement as another kind
Mode, when fluid flow paths have been upper and lower surface difference closed assembly cover board and the substrate of channel plate, and is limited by them jointly
During the pipeline that the space come is formed, fluid flow paths part on channel plate hollow out up and down, fluid flow paths by cover board and
The common closed assembly of substrate simultaneously limits gained, and liquid is flowed into conversion zone by the restriction passage.
Preferably, the conversion zone includes water wetted material.
When the conversion zone includes water wetted material, liquid sample can be attracted to flow into conversion zone from sample cavity so that
Liquid sample flowing is more rapid, realizes react into conversion zone more quickly.
It is further preferred that the tube wall of the circulation duct at least partially water wetted material, and the water wetted material portion
The both ends of fluid connection passage are run through in position.
No matter the liquid communication pipeline is to limit to be formed by above-mentioned closed assembly in a manner of which kind of, in general embodiment,
The fluid flow paths have tube wall (such as hollow out groove two side walls in itself and the lid that can be substantially expressed as on four direction
Plate, the part of substrate and the hollow out groove restriction, or be that non-hollow out groove in itself with described non-engrave by three tube walls and cover board
The part of empty groove restriction).As one of which preferred embodiment, four tube walls at least one be with hydrophilic material
What material made, it may be such that liquid is preferably flowed from the sample cavity toward the recirculation hole direction.When whole four tube walls
When being made of water wetted material, aforesaid liquid flow effect is more preferably.
On the basis of the above embodiment, the embodiment being more highly preferred to is the extensional pipeline bag of the strip
Containing hydrophobic material, or partly it is made by hydrophobic material.
In this way, hydrophilic segment applies pulling force so as to pull sample to flow forward to sample liquids.When sample passes through the stream
During through hole, its hydrophobic effect of the extensional pipeline can prevent sample from continuing to flow.
Preferably, the hydrophobic material is plastics, can be specifically PET either ABS or PC etc., as wherein one
Kind preferred embodiment, the water wetted material can be by carrying out hydrophilic treated come real on the hydrophobic material to its surface
It is existing, such as physical treatment, chemical treatment, sided corona treatment or other processing methods.Preferably, the cover board closed assembly is in channel plate
On, which is movable closed assembly or fixed closed assembly；Alternatively, the substrate closed assembly under channel plate, is somebody's turn to do
Closed assembly is movable closed assembly or fixed closed assembly.
It should be noted that between cover board and channel plate, the closed assembly between substrate and channel plate, can be movable closed assembly
Or fixed closed assembly, need to select closed assembly mode, the progress of convenient test according to test-types.The closed assembly can pass through
Various methods are realized, for example the objects such as buckle or screw can be used to bind together the part；Alternatively, it can pass through
Related component is sticked together using double faced adhesive tape；Or can be by heating (such as ultrasonic wave) by each parts surface portion
Divide fusing and then be sticked together.
Preferably, electrode is equipped with the conversion zone；Preferably, the conversion zone is also loaded with reaction examination in advance
Agent；It is highly preferred that the reaction reagent is dry reagent or passes through pretreatment into dry reagent.
It should be noted that electrode can be provided with according to the actual needs of detection in conversion zone so that liquid flows into anti-
Answer when being contacted in region with the electrode, energization can be reacted.Further, can be preset in conversion zone required
Reaction reagent so that liquid is flowed into the conversion zone can be reacted when being contacted with reaction reagent.Reaction reagent is preferred
Be dry reagent or pretreatment into dry reagent, prevent reaction reagent from being overflowed in the storage process of detection strip.
Preferably, one padded is additionally provided between the channel plate and the substrate, in the conversion zone
Side limits the conversion zone wall body increased.
It should be noted that it can be that testing liquid and reaction reagent carry out reaction and carry to limit the conversion zone wall body increased
For certain reaction compartment, to improve the effect reacted and detected.As a preferred embodiment, padded is that double faced adhesive tape is padded
Piece, is better achieved the fixation of padded, prevents the dislocation of padded from moving, prevents testing liquid to can not be successfully inflow reaction zone
In domain.
Preferably, the detection strip is blood testing strip；It is highly preferred that detect strip for prothrombin time.
It should be noted that prothrombin time detection strip can be prothrombin time (PT), activated partial blood coagulation
Movable enzyme time (aPTT), and other blood parameters detection strip, such as blood sugar test strip.
To realize above-mentioned 3rd purpose, the technical solution adopted in the present invention is as follows：
A kind of detecting instrument containing above-mentioned detection strip, also containing reaction signal collecting device or signal processing and analysis
Equipment, conducts to signal collecting device, or conduction for will detect physics, chemistry or electric signal after strip conversion zone reacts
To signal processing and analysis equipment and it is further analyzed analysis and the results show.
Preferably, the detecting instrument is factor detector.
Compared with prior art, the beneficial effects of the present invention are：
1st, communicated by the fluid flow paths on the gas outlet and channel plate on cover board and extension passage composition with the external world
Ventilation mouth, ventilation mouth is not arranged on directly over recirculation hole, and is arranged on extensional pipeline or the more far-end arranged on extensional pipeline end,
It may be such that blood sample to be measured is evenly more reliably full of conversion zone from sample cavity through fluid course, to improve the weight of test result
Renaturation and reliability.
2nd, by setting fluid flow paths and extension passage, can avoid during liquid fills conversion zone anti-
Region is answered to produce bubble, in order to avoid influence reaction effect.
3rd, preferable extensional pipeline cross-sectional area, fluid course cross-sectional area, distance of the ventilation mouth away from conversion zone with
And under ventilative open area, it can more preferably realize liquid flow effect.
4th, by setting padded between channel plate and substrate so that limit the reaction increased above conversion zone
Region wall body can provide the reaction compartment of bigger for the reaction of blood sample to be measured and reaction reagent, ensure good reaction effect.
Described above is only the general introduction of technical solution of the present invention, in order to better understand the technological means of the present invention,
And can be practiced according to the content of specification, and in order to allow the above and other objects, features and advantages of the present invention can
Become apparent, below especially exemplified by preferred embodiment, and coordinate attached drawing, describe in detail as follows.
Brief description of the drawings
Fig. 1 is the biological testing bar structure diagram of the prior art；
Fig. 2 is the structure diagram for the first structure that the present invention is applied to detection strip；
Fig. 3 is the structure diagram for second of structure that the present invention is applied to detection strip；
Fig. 4 is the structure diagram for the third structure that the present invention is applied to detection strip；
Fig. 5 is that the fluid flow paths of present invention detection strip are the left view of groove；
Fig. 6 is the water conservancy diversion design sketch that the experiment Distribution Test prior art detects strip A；
Fig. 7 is the water conservancy diversion design sketch of experiment Distribution Test present invention detection strip B；
In figure, 1, cover board；11st, sample holes；12nd, gas outlet；2nd, channel plate；21st, sample cavity；22nd, fluid flow paths；
23rd, recirculation hole；24th, extensional pipeline；3rd, substrate；31st, conversion zone；32nd, electrode；4th, padded；41st, padded through hole.
Embodiment
Further to illustrate the present invention to reach the technological means and effect that predetermined goal of the invention is taken, below in conjunction with
Attached drawing and preferred embodiment, to embodiment, structure, feature and its effect according to the present invention, describe in detail as follows：
The embodiment of the prior art
Fig. 1 is the structure diagram of the prior art.The biological testing bar includes：The substrate 101 of electrode is loaded with, is provided with
The reaction zone space of reaction reagent 104 and hydrophilic cover 109, further include between the double faced adhesive tape between substrate 101 and hydrophilic cover 109
Interlayer 105, the groove 108 formed with openings at one side on double faced adhesive tape wall 105, with the substrate 101 and hydrophilic cover 109
Limit and form an affiliated reaction zone space with sample holes 401 and exhausting-gas hole 107；Sample holes 401 are located at double faced adhesive tape one side
It is open at 106, exhausting-gas hole 107 is located on hydrophilic cover 109.Reaction zone space is arranged on one end of substrate 101, so can be straight
Connect from the finger of tester or other position blood-sample withdrawals from injection port 401 to the reaction zone space.Electrode includes working electrode
102 and reference electrode 103, testing liquid enters the reaction zone space through sample holes 401, is carried out with reaction reagent 104
Reaction, the electric signal for reacting generation are exported by working electrode and reference electrode through 110 connecting wire of electrode connection terminal.
Embodiment one
Fig. 2 is the structure diagram of the first embodiment of the invention, i.e. the structure for liquid sample detection strip.Liquid
The composed component of body pattern detection strip includes cover board 1, channel plate 2 and substrate 3.Wherein, sample holes 11 are arranged on a left side for cover board 1
End, gas outlet 12 are arranged on the right end of cover board 1.Channel plate 2 is equipped with sample cavity 21, the groove as fluid flow paths 22 and circulation
Hole 23.Groove is hollow out, when the upper and lower surface of channel plate distinguishes closed assembly cover board 1 and substrate 3, is limited jointly by them
The space come forms circulation duct, and the one side wall of pipeline is cover board lower surface, and another side wall is 3 upper surface of substrate.Wherein, into
Sample chamber 21 is arranged on the left end of channel plate 2, and when 1 closed assembly of cover board is on channel plate 2, sample cavity 21 be located in cover board 1 into
The underface in sample hole 11.
In addition, sample cavity 21 and recirculation hole 23 are connected by fluid flow paths 22 so that liquid can be through liquid communication
Passage 22 is flowed into recirculation hole 23 from sample cavity 21.Recirculation hole 23 is extended with fluid flow paths relative to the distal end of sample cavity 21
22 extensional pipeline 24.Gas outlet 12 on cover board 1 is located at the surface of the end of extensional pipeline 24, is limited with channel plate 2
Ventilation mouth (not marked in figure) is located at the end of extensional pipeline 24 so that extensional pipeline 24 is saturating by being limited by gas outlet 12
Gas port is in communication with the outside.
In the present embodiment, fluid flow paths 22 are the passage of about one hollow out, specifically channel plate 2 is upper
When lower surface difference closed assembly cover board 1 and substrate 3, limited jointly by them Lai the pipeline that is formed of space.
Substrate 3 is loaded with conversion zone 31 and electrode 32, has dry reaction reagent (not marked in figure) on conversion zone 31.
Conversion zone 31 is located at the underface of the recirculation hole 23 of channel plate 2.
Component for example above-mentioned set cover board 1, channel plate 2 and substrate 3 are by way of movable closed assembly, from the bottom to top successively
Closed assembly, forms liquid sample detection strip.
Conversion zone 31, which is more quickly flowed into, for guiding fluid sample realizes reaction, it is in preferred embodiments, described
Conversion zone 31 includes water wetted material, or the conversion zone 31 is made by water wetted material at least in part.
And in highly preferred embodiment, four tube wall (such as hollow out ditches possessed by the fluid flow paths 22
The two side walls of groove in itself and cover board 1, substrate 3 and the part of the hollow out groove restriction, or be non-hollow out groove in itself three
A tube wall and cover board 1 and the part of the non-hollow out groove restriction) at least one tube wall is made by water wetted material；
Certainly another alternative embodiment, at least described liquid communication pipeline include water wetted material, or portion at least in part
Ground is divided to be made by water wetted material；When four tube walls are all made of water wetted material, the effect above is more preferably.
And liquid sample flows out the gas outlet 12 from the extensional pipeline 24 in order to prevent, the quality of reaction is influenced,
In preferred embodiment, the extensional pipeline 24 includes hydrophobic material, or is made at least in part of hydrophobic material；Lean on
Nearly air outlet parts are hydrophobic material, and are water wetted material close to circulation bore portion.
In use, clicking and entering testing liquid sample in sample holes 11, liquid sample flows into sample cavity 21 from sample holes 11, is covering
Under the action of 1 water wetted material of plate, liquid sample flows into conversion zone 31 through fluid flow paths 22, in the process, due to prolonging
The ventilation mouth for stretching 24 end of pipeline is in communication with the outside by the gas outlet 12 on cover board 1 so that liquid can be in liquid flow all
Flowed forward in road 22.After testing liquid sample is full of conversion zone 31, extensional pipeline 24 will be then flowed into forward, directly
Hydrophobic part to extensional pipeline 24 prevents to flow by its hydrophobic effect.If the viscosity of fluid sample is larger, fluid sample has
Flowing may just be stopped before the hydrophobic part of extensional pipeline 24 is not reached, because being produced at this time by the hydrophilic interaction of cover board 1
Pulling force and the flow resistance of fluid sample reach balance.By in liquid sample detection strip insertion detecting instrument so that electrode
32 are powered.At this time, liquid sample, reaction reagent are reacted in the electrode 32 of energization.
Embodiment two
Fig. 3 is the structure diagram of second of embodiment of the invention, and present embodiment is the base in above-described embodiment one
One padded 4 is added on plinth, is similarly the structure for liquid sample detection strip.Specifically, described padded 4
The radial dimension of padded through hole 41 thereon is identical with the recirculation hole 23 on channel plate 2 and the conversion zone 31 on substrate 3.Pad
High piece 4 is fixed between channel plate 2 and substrate 3 by way of stickup, and causes recirculation hole 23, padded 41 and of through hole
Conversion zone 31 is located on same vertical plane.In preferred embodiments, in addition to conversion zone 31 is made by water wetted material,
3 remainder of substrate is made by hydrophobic material.Described padded 4 is used for limitation to conversion zone, in order to reagent point sample,
When required amount of reagent is larger, liquid is unlikely to overflow, and on the conversion zone 31 being limited on bottom plate 3.In addition, when tested
When sample flows into conversion zone 31, padded 4 can to participate in the sample size increase reacted.
Embodiment three
Fig. 4 is the structure diagram of the third embodiment of the invention, is similarly the knot for liquid sample detection strip
Structure.With embodiment two difference lies in, the gas outlet 12 on cover board 1 is not located at the surface of the end of extensional pipeline 24, and
It is that at this time, the ventilation mouth that gas outlet 12 is limited with channel plate 2 (does not mark) position in figure positioned at the surface at 24 midpoint of extensional pipeline
In the midpoint of extensional pipeline 24 so that extensional pipeline 24 is in communication with the outside by the ventilation mouth that gas outlet 12 limits.It is real herein
During the use for applying mode, since the ventilation mouth at 24 midpoint of extensional pipeline is in communication with the outside by the gas outlet 12 on cover board 1.
Setting by this way, may be such that blood sample to be measured is evenly more reliably full of from sample cavity 21 through fluid course
Conversion zone 31, preferably to improve the repeatability of test result and reliability, while can avoid filling conversion zone in liquid
Bubble is produced in conversion zone 31 during 31, preferably to exempt to influence reaction effect.
Example IV
It is lower section not hollow out such as embodiment 1, but as the groove of fluid flow paths 22.Fig. 5 is shown containing non-
The left view of the channel plate 2 of hollow out groove.By the setting of non-hollow out groove, when liquid flows in fluid flow paths 22,
It can smoothly be flowed into using channel bottom to rely in conversion zone 31.
Embodiment five
Circulate experiment test
In order to verify the advantage of structure of the present invention, the liquid mobility status by following experiment test after blood sample point sample,
And there is probability of occurrence of liquid bubble or underfill etc. in device.
Test method：
By detection strip A existing in the prior art and detection strip B of the invention, (concrete structure is as set by embodiment two
Put) Experimental comparison is carried out, wherein, structure differences of the detection strip A with detecting strip B essentially consists in, the ventilation mouth of detection strip A
Be arranged on recirculation hole, and the ventilation mouth for detecting strip B be arranged on extensional pipeline wherein on, remaining structure both of which
It is same to set, to form the effect of quantitative experiment.
In experimentation, to detection strip A and detect the sample cavity of strip B respectively and click and enter 10 μ l, blood sample, blood sample point
The fluid flow paths through strip are not flowed into conversion zone through recirculation hole.
Test result：
Fig. 6 and Fig. 7 shows the difference of the water conservancy diversion effect of this experiment detection strip A and detection strip B, in an experiment, such as
Shown in Fig. 6, the blood samples in strip A are detected during reaction chamber is filled, it can be clearly seen that vortex or turbulent flow, also
The discontented phenomenon of filling occurs.In contrast to this, as shown in fig. 7, blood sample in detection strip B can very uniformly, it is steady,
Reliably it is full of reaction chamber.
In addition, showing through experiment is repeated several times, detection strip B can ensure that blood sample 100% is equal during water conservancy diversion
It is even to be smoothly full of reaction chamber, and detect strip A and bubble or the phenomenon not being full of then occurs, its incidence up to 5% or with
On.
Also, when strip using the present invention is detected, blood sample from point sample to full of needed for reaction zone when
Between it is shorter, 4-5s can be full of reaction zone, can further improve signal quality.
Provable by above-mentioned circulation experiment test, liquid sample provided by the present invention detects strip, may be such that to be measured
Blood sample is evenly more reliably full of conversion zone from sample cavity through fluid course, to improve the repeated and reliable of test result
Property, while can avoid producing bubble in conversion zone during liquid fills conversion zone, reaching does not influence reaction effect
Technical purpose.
The above embodiment is only the preferred embodiment of the present invention, it is impossible to the scope of protection of the invention is limited with this,
The change and replacement for any unsubstantiality that those skilled in the art is done on the basis of the present invention belong to institute of the present invention
Claimed scope.
Claims (15)
1. a kind of liquid sample guiding device, including cover board and the overlappable channel plate below cover board, one end on the cover board
Equipped with sample holes, the other end is equipped with gas outlet；The channel plate is equipped with sample cavity, fluid flow paths and recirculation hole, wherein, when
When the cover board closed assembly is on the channel plate, the sample cavity is located at the lower section of the sample holes, and passes through the liquid
Circulation passage connects the recirculation hole, it is characterised in that the recirculation hole of the channel plate is also associated with extensional pipeline, it prolongs
Stretch direction be relative to the sample cavity and the liquid communication pipeline other side, when the cover board closed assembly in channel plate it
When upper, the channel plate limits a ventilation mouth communicated with the external world by gas outlet, and the ventilation mouth is located at the extension
On any point of pipeline, or the ventilation mouth is at least partly contacted with the extensional pipeline.
2. guiding device as claimed in claim 1, it is characterised in that the aperture of the gas outlet is 0.1mm-5mm；Preferably,
The ratio between cross-sectional area of the cross-sectional area of the extensional pipeline and the fluid flow paths is 1:1–1:10.
3. guiding device as claimed in claim 1, it is characterised in that the length of the extensional pipeline and the fluid flow paths
Length ratio be 1:1–1:10.
4. guiding device as claimed in claim 1, it is characterised in that the tube wall of the fluid flow paths is at least partially hydrophilic
Material, and the both ends of fluid connection passage are run through at the water wetted material position.
5. guiding device as claimed in claim 1, it is characterised in that at least partially hydrophobic material of the tube wall of the extensional pipeline
Material；It is hydrophobic material to be preferably close to air outlet parts, and is water wetted material close to circulation bore portion；Preferably, water wetted material
Partial length is 1-2mm..
6. guiding device as claimed in claim 1, it is characterised in that the sample cavity, the fluid flow paths, the circulation
Hole, the extensional pipeline and the ventilation mouth are respectively positioned on same straight line.
7. guiding device as claimed in claim 1, it is characterised in that the sample cavity is located at the underface of the sample holes.
8. the guiding device as described in claim 1-7 is any, it is characterised in that distance of the ventilation mouth away from the end be
1mm–10mm；Preferably, the area of the ventilation mouth and the area ratio of the sample holes are 1:1–1:100.
9. a kind of liquid sample detects strip, it is characterised in that described containing the guiding device as described in claim 1-7 is any
Strip also contains substrate, and the substrate is equipped with conversion zone, when the substrate closed assembly is under the channel plate, the stream
Through hole is located on the conversion zone and least partially overlapped with it.
10. detection strip as claimed in claim 9, it is characterised in that the fluid flow paths are groove；Preferably, it is described
Groove is non-hollow out or hollow out, when the groove is hollow out, and when the upper and lower surface difference closed assembly of channel plate
When cover board and substrate, limited jointly by them Lai space form circulation duct；Preferably, the conversion zone includes parent
Water material；It is further preferred that the tube wall of the circulation duct at least partially water wetted material, and the water wetted material position
Through the both ends of fluid connection passage.
11. detection strip as claimed in claim 9, it is characterised in that the cover board closed assembly is on the channel plate, the base
Plate closed assembly is under the channel plate.
12. detection strip as claimed in claim 9, it is characterised in that electrode is equipped with the conversion zone；Preferably, it is described
Conversion zone is also loaded with reaction reagent in advance；It is highly preferred that the reaction reagent is dry reagent or passes through pretreatment into dry examination
Agent.
13. detection strip as claimed in claim 10 a, it is characterised in that use is additionally provided between the channel plate and the substrate
In limiting padded of the conversion zone wall body increased above the conversion zone.
14. detection strip as claimed in claim 9, it is characterised in that the detection strip is blood testing strip；Preferably,
The blood testing strip detects strip for prothrombin time.
15. a kind of detecting instrument containing detection strip as claimed in claim 9, it is characterised in that also adopted containing reaction signal
Collect equipment or signal processing and analysis equipment, for the physical/chemical after the conversion zone reaction of the detection strip to be believed
Number conduction is to signal collecting device, or conduction to the signal processing and analysis equipment and is further analyzed analysis and result is shown
Show；Preferably, the detecting instrument is prothrombin time detector.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610887868.7A CN107918031A (en) | 2016-10-11 | 2016-10-11 | A kind of liquid sample guiding device and the detection device containing the guiding device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610887868.7A CN107918031A (en) | 2016-10-11 | 2016-10-11 | A kind of liquid sample guiding device and the detection device containing the guiding device |
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| CN107918031A true CN107918031A (en) | 2018-04-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610887868.7A Pending CN107918031A (en) | 2016-10-11 | 2016-10-11 | A kind of liquid sample guiding device and the detection device containing the guiding device |
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| CN109540881A (en) * | 2018-12-06 | 2019-03-29 | 桂林优利特医疗电子有限公司 | A kind of analoids |
| CN112442440A (en) * | 2019-08-30 | 2021-03-05 | 台达电子工业股份有限公司 | Biological detection cassette and method |
| CN113008952A (en) * | 2019-12-20 | 2021-06-22 | 利多(香港)有限公司 | Biosensor for sample detection |
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Effective date of registration: 20200603 Address after: 510663 unit 901, zone C2, No. 182, science Avenue, Science City, Guangzhou high tech Industrial Development Zone, Guangzhou, Guangdong Province Applicant after: HELIXGEN (GUANGZHOU) Co.,Ltd. Address before: Unit 901, zone C2, innovation building, No. 182, science Avenue, Guangzhou Applicant before: Zhao Tianxian Applicant before: HELIXGEN (GUANGZHOU) Co.,Ltd. |
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Application publication date: 20180417 |