CN105921187A - Micro-fluidic chip for rapid detection of pesticide residues - Google Patents
Micro-fluidic chip for rapid detection of pesticide residues Download PDFInfo
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- CN105921187A CN105921187A CN201610355514.8A CN201610355514A CN105921187A CN 105921187 A CN105921187 A CN 105921187A CN 201610355514 A CN201610355514 A CN 201610355514A CN 105921187 A CN105921187 A CN 105921187A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502723—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by venting arrangements
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
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Abstract
The invention provides a micro-fluidic chip for rapid detection of pesticide residues. The micro-fluidic chip is composed of a cover plate layer, a middle layer and a bottom plate layer. A cover plate comprises a sample inlet and a vent hole. The middle layer comprises a sample injection channel, an enzyme reaction pond, a connection channel, a developing pond, a ventilation channel, a cholinesterase paper sheet and an indophenols acetate paper sheet. The sample injection channel is connected with the sample inlet and the enzyme reaction pond, the connection channel is connected with the enzyme reaction pond and the developing pond, the ventilation channel is connected with the developing pond and the vent hole, the cholinesterase paper sheet is located in the enzyme reaction pond, the indophenols acetate paper sheet is located in the developing pond, and driving force of liquid in the chip is air pressure. During use, the liquid firstly reacts with the cholinesterase paper sheet and then reacts with the indophenols acetate paper sheet, and finally the concentration of pesticide in the liquid is determined through the color change of the indophenols acetate paper sheet. The micro-fluidic chip is characterized in that a traditional pesticide residue rapid detection card is combined with the micro-fluidic technological chip, and the accuracy of the detection result of the traditional rapid detection card is remarkably improved.
Description
Technical field
The present invention relates to micro fluidic chip technical field, a kind of micro-fluidic chip for Fast Determination of Pesticide Residue.
Background technology
Organophosphorus and carbamate pesticides class pesticide acetylcholine esterase is had inhibitory action, therefore, pesticide residues quickly detect main base
In inhibiting AChE, including quick measuring card method (dry chemical method) and enzyme inhibition rate method (wet chemistry method) based on spectrophotography.Based on
Although the enzyme inhibition rate method testing result of spectrophotography is more accurate, but owing to detection needs possess spectrophotometer, unfavorable
In field quick detection.Although card method is without specialized equipment, but simple to operate quickly, be directly incorporated into scraps of paper two
Two kinds of tablets carry out doubling pressing reaction solution, frequently result in testing result false positive or false negative is too high.
Therefore, in the field quick detection of pesticide residues, need a kind of operation i.e. simple result detection method the most accurately.
Summary of the invention
It is an object of the invention to by microfluidic chip technology platform, in conjunction with tradition thick salt method, by dry chemical method and humidifying
Be combined with each other, and finally realizes the quick and precisely detection of pesticide residues.
In order to realize object above, the invention provides a kind of micro-fluidic chip for Fast Determination of Pesticide Residue, chip utilizes
The change of air pressure drives liquid to flow in chip, it is achieved the quick and precisely detection of Organophosphorus and carbamate pesticides class pesticide.
A kind of micro-fluidic chip for Fast Determination of Pesticide Residue that the present invention provides, by cover plate layer, intermediate layer and backsheet layer group
Becoming, described cover plate includes that injection port and blow vent, described intermediate layer include sample intake passage, enzyme reaction pond, interface channel, colour developing
Pond, venting channels, the acetylcholine esterase scraps of paper and the indophenols acetas scraps of paper, described sample intake passage connects injection port and enzyme reaction pond, even
Connecting road ligase reaction tank and connect colour developing pond and blow vent with colour developing pond, venting channels, the acetylcholine esterase scraps of paper are positioned at enzyme reaction pond,
The indophenols acetas scraps of paper are positioned at colour developing pond.
Described cover plate layer is any of the high molecular polymers such as glass, resin and polyethylene, polypropylene, polymethyl methacrylate
One or more transparent materials form, intermediate layer and backsheet layer be pottery, glass, resin, metal and polyethylene, polypropylene,
One or more materials composition of the high molecular polymers such as polymethyl methacrylate.
The described acetylcholine esterase scraps of paper and the indophenols acetas scraps of paper be filter paper, absorbent paper, microporous sponge, polymeric membrane etc. all have
The sheeting of absorbability is combined obtains with acetylcholine esterase and indophenols acetas respectively, and manufacturing process can be by described sheeting
First it is fixed in chip respectively in connection with after also cutting with acetylcholine esterase and indophenols acetas, it is possible to sheeting is first fixed on chip
Dropping acetylcholine esterase and indophenols acetas are combined the most respectively.
In chip, the driving force of liquid flowing is air pressure, can be malleation, it is possible to be negative pressure, it is achieved the instrument that air pressure changes is permissible
It is suction pipe, pipettor or other air Micropumps.
During use, liquid to be checked arrives enzyme reaction pond from injection port by sample intake passage and infiltrates the acetylcholine esterase scraps of paper and react, then pump
The pond that enters to develop the color is reacted with the indophenols acetas scraps of paper, and whole detection course of reaction can be static, it is also possible to shakes, rock, finally leads to
The color change crossing the indophenols acetas scraps of paper determines the concentration range of liquid Pesticides to be checked, the wherein color of the indophenols acetas scraps of paper
Change can perusal, may also be combined with digital image processing techniques read.
The innovative point of the present invention is: combined with microfluidic chip technology by the pesticide residue fast detection card using dry chemical method, it is achieved
Dry chemical method and effective combination of wet chemistry method, significantly improve the accuracy of tradition quick measuring card testing result, it is achieved that pesticide
The scene of residual is quick and precisely detected.
Accompanying drawing explanation
Fig. 1 is a kind of microfluidic chip structure schematic diagram for Fast Determination of Pesticide Residue provided by the present invention.Wherein 1:
Cover plate layer；2: intermediate layer；3: backsheet layer；4: blow vent；5: injection port.
A kind of Fast Determination of Pesticide Residue micro-fluidic chip knot combining dry chemical method and wet chemistry method that Fig. 2 provides for the present invention
Structure floor map.6: enzyme reaction pond；7: colour developing pond；8: the indophenols acetas scraps of paper；9: the acetylcholine esterase scraps of paper；10: logical
Gas passage；11: interface channel；12: sample intake passage.
Detailed description of the invention
Below in conjunction with Fig. 1 and Fig. 2, a kind of micro-fluidic chip for Fast Determination of Pesticide Residue that the present invention is provided make into
One step explanation.
Embodiment 1
A kind of micro-fluidic chip for Fast Determination of Pesticide Residue, is made up of cover plate layer, intermediate layer and backsheet layer, described cover plate
Including injection port and blow vent, described intermediate layer include sample intake passage, enzyme reaction pond, interface channel, colour developing pond, venting channels,
The acetylcholine esterase scraps of paper and the indophenols acetas scraps of paper, described sample intake passage connects injection port and enzyme reaction pond, and interface channel ligase is anti-
Ying Chiyu develops the color pond, and venting channels connects colour developing pond and blow vent, and the acetylcholine esterase scraps of paper are positioned at enzyme reaction pond, indophenols acetas paper
Sheet is positioned at colour developing pond.
Described cover plate layer is any of the high molecular polymers such as glass, resin and polyethylene, polypropylene, polymethyl methacrylate
One or more transparent materials form, intermediate layer and backsheet layer be pottery, glass, resin, metal and polyethylene, polypropylene,
One or more materials composition of the high molecular polymers such as polymethyl methacrylate.
The described acetylcholine esterase scraps of paper and the indophenols acetas scraps of paper be filter paper, absorbent paper, microporous sponge, polymeric membrane etc. all have
The sheeting of absorbability is combined obtains with acetylcholine esterase and indophenols acetas respectively, manufacturing process first by described sheeting with
Acetylcholine esterase and indophenols acetas respectively in connection with and cutting after be fixed in chip, it is possible to sheeting is first fixed in chip again
Dropping acetylcholine esterase and indophenols acetas are combined respectively.
During application, first soak agricultural product certain time acquisition extracting solution with buffer, then with pipettor or suction pipe, extracting solution is led to
Crossing injection port to add in chip, extracting solution, in sample intake passage enters enzyme reaction pond, then use hand wobble chip, make extracting solution and
The acetylcholine esterase scraps of paper fully infiltrate and react, and after 10 minutes, are directed at injection port with pipettor or suction pipe and pump into air, by liquid
Pump into colour developing pond by interface channel fully infiltrate with the indophenols acetas scraps of paper and complete chromogenic reaction, again use hand wobble chip,
Make liquid and the indophenols acetas scraps of paper fully infiltrate and react 3 minutes, then observing shade and directly determine whether that pesticide is residual
Stay.
Embodiment 2
A kind of micro-fluidic chip for Fast Determination of Pesticide Residue, is made up of cover plate layer, intermediate layer and backsheet layer, described cover plate
Including injection port and blow vent, described intermediate layer include sample intake passage, enzyme reaction pond, interface channel, colour developing pond, venting channels,
The acetylcholine esterase scraps of paper and the indophenols acetas scraps of paper, described sample intake passage connects injection port and enzyme reaction pond, and interface channel ligase is anti-
Ying Chiyu develops the color pond, and venting channels connects colour developing pond and blow vent, and the acetylcholine esterase scraps of paper are positioned at enzyme reaction pond, indophenols acetas paper
Sheet is positioned at colour developing pond.
Described cover plate layer is any of the high molecular polymers such as glass, resin and polyethylene, polypropylene, polymethyl methacrylate
One or more transparent materials form, intermediate layer and backsheet layer be pottery, glass, resin, metal and polyethylene, polypropylene,
One or more materials composition of the high molecular polymers such as polymethyl methacrylate.
The described acetylcholine esterase scraps of paper and the indophenols acetas scraps of paper be filter paper, absorbent paper, microporous sponge, polymeric membrane etc. all have
The sheeting of absorbability is combined obtains with acetylcholine esterase and indophenols acetas respectively, and manufacturing process can be by described sheeting
First it is fixed in chip respectively in connection with after also cutting with acetylcholine esterase and indophenols acetas, it is possible to sheeting is first fixed on chip
Dropping acetylcholine esterase and indophenols acetas are combined the most respectively.
During application, first soak agricultural product certain time acquisition extracting solution with buffer, then with pipettor or suction pipe, extracting solution is led to
Crossing injection port to add in chip, extracting solution, in sample intake passage enters enzyme reaction pond, then use hand wobble chip, make extracting solution and
The acetylcholine esterase scraps of paper fully infiltrate and react, and after 10 minutes, are directed at blow vent with pipettor or suction pipe and take air in colour developing pond away
Making colour developing pond form negative pressure drives liquid to complete chromogenic reaction from entrance colour developing pond, enzyme reaction pond with indophenols acetas sheet, again uses
Hand wobble chip, makes liquid and the indophenols acetas scraps of paper fully infiltrate and reacts 3 minutes, then using the hands with camera function
Machine is taken pictures and is combined the amount of the preassembled software analysis pesticide residues with Digital Image Processing function of mobile phone.
These are only two kinds of embodiments of the present invention, in this area, other embodiments obtained without innovation belong to this
Bright protection domain.
Claims (4)
1. the micro-fluidic chip for Fast Determination of Pesticide Residue, it is characterised in that: described micro-fluidic chip by cover plate layer,
Intermediate layer and backsheet layer composition, described cover plate include injection port and blow vent, described intermediate layer include sample intake passage, enzyme reaction pond,
Interface channel, colour developing pond, venting channels, the acetylcholine esterase scraps of paper and the indophenols acetas scraps of paper, described sample intake passage connects injection port
With enzyme reaction pond, interface channel ligase reaction tank connects colour developing pond and blow vent, acetylcholine esterase paper with colour developing pond, venting channels
Sheet is positioned at enzyme reaction pond, and the indophenols acetas scraps of paper are positioned at colour developing pond.
Micro-fluidic chip the most according to claim 1, it is characterised in that: described cover plate layer be glass, resin and polyethylene,
Any one or more transparent material composition of the high molecular polymer such as polypropylene, polymethyl methacrylate, intermediate layer and egative film
Layer is the one of the high molecular polymers such as pottery, glass, resin, metal and polyethylene, polypropylene, polymethyl methacrylate
Or multiple material composition.
Micro-fluidic chip the most according to claim 1, it is characterised in that: the described acetylcholine esterase scraps of paper and indophenols acetas paper
Sheet be filter paper, absorbent paper, microporous sponge, polymeric membrane etc. all have the sheeting of absorbability respectively with acetylcholine esterase and
Indophenols acetas combines and obtains.
Micro-fluidic chip the most according to claim 1, it is characterised in that: in chip, the driving force of liquid flowing is air pressure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610355514.8A CN105921187A (en) | 2016-05-23 | 2016-05-23 | Micro-fluidic chip for rapid detection of pesticide residues |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610355514.8A CN105921187A (en) | 2016-05-23 | 2016-05-23 | Micro-fluidic chip for rapid detection of pesticide residues |
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| CN105921187A true CN105921187A (en) | 2016-09-07 |
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| CN201610355514.8A Pending CN105921187A (en) | 2016-05-23 | 2016-05-23 | Micro-fluidic chip for rapid detection of pesticide residues |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107144559A (en) * | 2017-04-20 | 2017-09-08 | 江苏大学 | Pesticide residue detection device and method based on multilayer papery micro-fluidic chip |
| CN107478648A (en) * | 2017-08-25 | 2017-12-15 | 东莞市恒度检测科技有限公司 | A kind of red of pesticide residue fast detection card and preparation method thereof |
| CN107597219A (en) * | 2017-09-13 | 2018-01-19 | 北京理工大学 | A kind of micro-fluidic chip sample introduction and its bonding techniques |
| CN107918031A (en) * | 2016-10-11 | 2018-04-17 | 赵天贤 | A kind of liquid sample guiding device and the detection device containing the guiding device |
| WO2018196265A1 (en) * | 2017-04-27 | 2018-11-01 | 京东方科技集团股份有限公司 | Genetic testing chip, testing method and manufacturing method therefor, and microfluidic chip system |
| CN109847818A (en) * | 2019-03-08 | 2019-06-07 | 北京理工大学 | A kind of high throughput microarray detection chip and preparation method thereof, application method |
| CN110196249A (en) * | 2018-02-27 | 2019-09-03 | 浙江清华长三角研究院 | A kind of paper chip of integration standard colour band |
| CN110470856A (en) * | 2018-05-11 | 2019-11-19 | 中国石油化工股份有限公司 | The manual injector and sample injection method of controllable sampling volume and its application |
| CN110907445A (en) * | 2019-12-06 | 2020-03-24 | 西安外事学院 | Microfluidic biochip detection device, preparation method and detection method |
| CN118048236A (en) * | 2024-02-05 | 2024-05-17 | 中国人民解放军军事科学院军事医学研究院 | Cholinesterase inhibitor detection chip and preparation and detection method thereof |
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| CN205056043U (en) * | 2015-08-24 | 2016-03-02 | 杭州霆科生物科技有限公司 | A disc micro -fluidic chip for in batches quick examination of pesticide residue |
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| CN103865754A (en) * | 2014-03-12 | 2014-06-18 | 杭州霆科生物科技有限公司 | Microfluidic-based enzyme inhibition reaction platform and analysis method |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107918031A (en) * | 2016-10-11 | 2018-04-17 | 赵天贤 | A kind of liquid sample guiding device and the detection device containing the guiding device |
| CN107144559B (en) * | 2017-04-20 | 2020-06-09 | 江苏大学 | Pesticide residue detection device and method based on multilayer paper microfluidic chip |
| CN107144559A (en) * | 2017-04-20 | 2017-09-08 | 江苏大学 | Pesticide residue detection device and method based on multilayer papery micro-fluidic chip |
| WO2018196265A1 (en) * | 2017-04-27 | 2018-11-01 | 京东方科技集团股份有限公司 | Genetic testing chip, testing method and manufacturing method therefor, and microfluidic chip system |
| CN107478648B (en) * | 2017-08-25 | 2019-11-15 | 东莞市恒度检测科技有限公司 | A kind of pesticide residue fast detection card red and preparation method thereof |
| CN107478648A (en) * | 2017-08-25 | 2017-12-15 | 东莞市恒度检测科技有限公司 | A kind of red of pesticide residue fast detection card and preparation method thereof |
| CN107597219A (en) * | 2017-09-13 | 2018-01-19 | 北京理工大学 | A kind of micro-fluidic chip sample introduction and its bonding techniques |
| CN110196249A (en) * | 2018-02-27 | 2019-09-03 | 浙江清华长三角研究院 | A kind of paper chip of integration standard colour band |
| CN110470856A (en) * | 2018-05-11 | 2019-11-19 | 中国石油化工股份有限公司 | The manual injector and sample injection method of controllable sampling volume and its application |
| CN109847818A (en) * | 2019-03-08 | 2019-06-07 | 北京理工大学 | A kind of high throughput microarray detection chip and preparation method thereof, application method |
| CN110907445A (en) * | 2019-12-06 | 2020-03-24 | 西安外事学院 | Microfluidic biochip detection device, preparation method and detection method |
| CN118048236A (en) * | 2024-02-05 | 2024-05-17 | 中国人民解放军军事科学院军事医学研究院 | Cholinesterase inhibitor detection chip and preparation and detection method thereof |
| CN118048236B (en) * | 2024-02-05 | 2024-07-23 | 中国人民解放军军事科学院军事医学研究院 | Cholinesterase inhibitor detection chip and preparation and detection method thereof |
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