CN104793004A - Pesticide residue detection micro-fluidic chip with pre-stored reaction reagent - Google Patents
Pesticide residue detection micro-fluidic chip with pre-stored reaction reagent Download PDFInfo
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Abstract
The invention provides a pesticide residue detection micro-fluidic chip with a pre-stored reaction reagent. The pesticide residue detection micro-fluidic chip is characterized by comprising a micro-fluidic channel, a liquid sac pool, an extracting solution sac, a sample pool, a sample pool cover, a plurality of reagent pools and bio-reagents stored in the reagent pools. During detection, a to-be-detected sample just needs to be put into the sample pool of the chip, a reagent solution such as an extracting solution, an enzyme liquid, a color developing agent solution or a primer solution does not need to be prepared. The chip is concise to operate and suitable for field detection of pesticide residues and has an extremely important application value.
Description
Technical field
The present invention relates to microfluidic chip analysis field, particularly a kind of micro-fluidic chip for residues of pesticides Site Detection.
Background technology
At present, microfluidic chip analysis technology, because having microminiaturization, integrated, the remarkable advantage such as high flux, low cost, is applied to the quick detection of Organic pesticide residues.But when the residual Site Detection of agriculture, still need to carry or configure various reaction reagent solution as extract, enzyme liquid, chromogenic reagent solution, substrate solution, be transferred in micro-fluidic chip by manual for all kinds of solution again, increase analyst's manipulation strength, and solution carry inconvenience; Layoutprocedure is tediously long, can not meet the demand of Fast Determination of Pesticide Residue, becomes the bottleneck that microfluidic analysis technology is applied on agricultural product speed survey field.
Summary of the invention
The object of the invention is to the above-mentioned shortcoming for current Detecting Pesticide micro-fluidic chip, a kind of Detecting Pesticide micro-fluidic chip of pre-stored reaction reagent is provided, structure is simple, cost is low, be beneficial to batch production, reaction biochemical reagents have prestored and have been stored among chip, are applicable to the Site Detection of residues of pesticides.
For achieving the above object, the invention provides a kind of Detecting Pesticide micro-fluidic chip of pre-stored reaction reagent, it is characterized in that, this chip comprises the biochemical reagents stored in microfluidic channel, liquid Nang Chi, extract liquid capsule, sample cell, sample cell lid, some reagent and reagent, only testing sample need be put into chip sample pond during detection, without the need to configuring reagent solution as extract, enzyme liquid, chromogenic reagent solution, substrate solution.
The invention provides a kind of Detecting Pesticide micro-fluidic chip of pre-stored reaction reagent, the biochemical reagents that described micro-fluidic chip inside prestores are enzyme, substrate and developer etc.; Described extract is phosphate buffer.
The invention provides a kind of Detecting Pesticide micro-fluidic chip of pre-stored reaction reagent, described reagent is the reaction tank of biochemical reaction simultaneously.
The invention provides a kind of Detecting Pesticide micro-fluidic chip of pre-stored reaction reagent, described biochemical reagents can be the solid reagents of Powdered, film-form or bulk; Described storage mode can be finishing, gel embedding, block encapsulation etc.
The invention provides a kind of Detecting Pesticide micro-fluidic chip of pre-stored reaction reagent, described extract adopts liquid to be encapsulated dress, and liquid capsule can be glass material, also can be organic polymer film material.
The invention provides a kind of Detecting Pesticide micro-fluidic chip of pre-stored reaction reagent, nadel structure is equipped with in the front end of described sample cell lid, and in sealed sample pond simultaneously, the broken liquid capsule of nadel structure realizes the release that body gets liquid.
The invention provides a kind of Detecting Pesticide micro-fluidic chip of pre-stored reaction reagent, in sample cell, sample is added during detection, fastening sample cell lid, extract in release liquid capsule, extract flows into sample cell and mixes with testing sample and extract, mixed liquor mixes with pre-stored reagent respectively, reacts and develops the color subsequently, calculates the residual inhibiting rate of agriculture finally by measurement solution absorbance value.
The invention provides a kind of Detecting Pesticide micro-fluidic chip of pre-stored reaction reagent, the pressure differential that the flowing of described liquid in chip can be produced by chip internal chamber and air drives, and also drives by external mechanical force.
The invention provides a kind of Detecting Pesticide micro-fluidic chip of pre-stored reaction reagent, only need testing sample to add chip during detection, avoid solution preparation work loaded down with trivial details in a large number, operation is succinct, be applicable to the Site Detection of residues of pesticides, there is extremely important using value.
Accompanying drawing explanation
Fig. 1. be the Detecting Pesticide microfluidic chip structure schematic diagram of a kind of pre-stored reaction reagent provided by the invention.In figure, each mark is as follows: 1 liquid Nang Chi, 2 fluid passages, 3 sample cells, 4 reagent 1,5 reagent 2, the 6 liquid capsule, 7 be stored in the liquid capsule pond enzymes, 8 be stored in reagent are stored in substrate in reagent and developer potpourri, 9 air holes.
Fig. 2. be the Detecting Pesticide micro-fluidic chip cross-sectional view of a kind of pre-stored reaction reagent provided by the invention.In figure, each mark is as follows: 1 liquid Nang Chi, 2 fluid passages, 3 sample cells, 4 reagent 1,5 reagent 2, the 6 liquid capsule, 7 be stored in the liquid capsule pond enzymes, 8 be stored in reagent are stored in substrate in reagent and developer potpourri, 10 with the sample cell lid of nadel.
Fig. 3 is the Detecting Pesticide microfluidic chip structure schematic diagram of another kind of pre-stored reaction reagent provided by the invention.In figure, each mark is as follows: 1 liquid Nang Chi, 2 fluid passages, 3 sample cells, 4 reagent 1,5 reagent 2, the 6 liquid capsule, 7 be stored in the liquid capsule pond enzymes, 8 be stored in reagent are stored in substrate in reagent and developer potpourri, 9 air holes.
Specific embodiments
The following examples will be further described the present invention, but not thereby limiting the invention.
As shown in Figure 1, the Detecting Pesticide micro-fluidic chip of pre-stored reaction reagent provided by the invention comprises liquid Nang Chi, liquid capsule, sample cell, microfluidic channel, 2 reagent and reagent.
Only quantitative testing sample need be put into chip sample pond 3 during detection, close sample chamber gland bonnet 10, extract 6 in release liquid capsule, air hole 9 connects the flowing that pressure differential that external pressure variable valve makes chip internal chamber and air produce drives liquid, extract enters sample cell 3 and mixes with testing sample and extract, mixed liquor enters in reagent 4 subsequently, enter in reagent 5 with the abundant hybrid reaction of pre-stored reagent 7, there is chromogenic reaction with the mix reagent 8 of pre-stored, finally measure solution absorbance value and calculate the residual inhibiting rate of agriculture.
As shown in Figure 3, the Detecting Pesticide micro-fluidic chip of pre-stored reaction reagent provided by the invention comprises liquid Nang Chi, liquid capsule, sample cell, microfluidic channel, 2 reagent and reagent.
Only quantitative testing sample need be put into chip sample pond 3 ' during detection, close sample chamber gland bonnet 10 ', extract 6 ' in release liquid capsule, chip is fixed on hydro-extractor, centrifugal force drives liquid flow, extract enters sample cell 3 ' and mixes with testing sample and extract, mixed liquor enters in reagent 4 ' subsequently, enter in reagent 5 ' with the abundant hybrid reaction of pre-stored reagent 7 ', there is chromogenic reaction with the mix reagent 8 ' of pre-stored, finally measure solution absorbance value and calculate the residual inhibiting rate of agriculture.
Claims (9)
1. the Detecting Pesticide micro-fluidic chip of a pre-stored reaction reagent, it is characterized in that, this chip comprises the biochemical reagents stored in microfluidic channel, liquid Nang Chi, extract liquid capsule, sample cell, sample cell lid, some reagent and reagent, only testing sample need be put into chip sample pond during detection, without the need to configuring reagent solution as extract, enzyme liquid, chromogenic reagent solution, substrate solution.
2. the Detecting Pesticide micro-fluidic chip of a kind of pre-stored reaction reagent as claimed in claim 1, it is characterized in that, in sample cell, sample is added during detection, fastening sample cell lid, extract in release liquid capsule, extract mixes with testing sample and extracts, and mixed liquor mixes with pre-stored reagent respectively, reacts and develops the color subsequently, calculates the residual inhibiting rate of agriculture finally by measurement solution absorbance value.
3. the Detecting Pesticide micro-fluidic chip of a kind of pre-stored reaction reagent as claimed in claim 1, is characterized in that, the biochemical reagents that described micro-fluidic chip inside prestores are enzyme, substrate and developer etc.; Extract in described liquid capsule is phosphate buffer.
4. the Detecting Pesticide micro-fluidic chip of a kind of pre-stored reaction reagent as claimed in claim 1, is characterized in that, biochemical reagents can be the solid reagents of Powdered, film-form or bulk; Described storage mode can be finishing, gel embedding, block encapsulation etc.
5. the Detecting Pesticide micro-fluidic chip of a kind of pre-stored reaction reagent as claimed in claim 1, is characterized in that, liquid capsule can be glass material, also can be organic polymer film material.
6. the Detecting Pesticide micro-fluidic chip of a kind of pre-stored reaction reagent as claimed in claim 1, is characterized in that, described reagent is the reaction tank of biochemical reagent reacting simultaneously.
7. the Detecting Pesticide micro-fluidic chip of a kind of pre-stored reaction reagent as claimed in claim 1, it is characterized in that, nadel structure is equipped with in the front end of described sample cell lid, and in sealed sample pond simultaneously, the broken liquid capsule of nadel structure realizes the release that body gets liquid.
8. the Detecting Pesticide micro-fluidic chip of a kind of pre-stored reaction reagent as claimed in claim 1, is characterized in that, the pressure differential that the flowing of liquid in chip can be produced by chip internal chamber and air drives, and also drives by external mechanical force.
9. the Detecting Pesticide micro-fluidic chip of a kind of pre-stored reaction reagent as claimed in claim 1, it is characterized in that, only need testing sample to add chip during detection, avoid solution preparation work loaded down with trivial details in a large number, operation is succinct, is applicable to the Site Detection of residues of pesticides.
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| CN201510208714.6A CN104793004A (en) | 2015-04-24 | 2015-04-24 | Pesticide residue detection micro-fluidic chip with pre-stored reaction reagent |
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Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105242053A (en) * | 2015-09-30 | 2016-01-13 | 江苏农林职业技术学院 | Micro-fluidic chip, and intelligent experiment system and method used for micro-fluidic chip |
| CN105353144A (en) * | 2015-10-14 | 2016-02-24 | 江苏理工学院 | Micro-fluidic intelligent experiment apparatus and working method thereof |
| CN105921187A (en) * | 2016-05-23 | 2016-09-07 | 浙江清华长三角研究院 | Micro-fluidic chip for rapid detection of pesticide residues |
| CN106053859A (en) * | 2016-08-02 | 2016-10-26 | 杭州霆科生物科技有限公司 | Centrifugal type glycosylated hemoglobin detection micro-fluidic chip |
| CN106119085A (en) * | 2016-08-23 | 2016-11-16 | 闫维新 | A kind of real-time fluorescence PCR mixing microchannel chip |
| CN106526178A (en) * | 2016-12-08 | 2017-03-22 | 刘宏川 | Continuous quantitative readable detection device adopting immunochromatography |
| CN106525724A (en) * | 2016-10-26 | 2017-03-22 | 杭州霆科生物科技有限公司 | Centrifugal fully integrated detection system of methanal and pH value |
| CN107129930A (en) * | 2017-06-09 | 2017-09-05 | 北京百康芯生物科技有限公司 | A kind of fully integrated detection of nucleic acids micro-fluidic chip and its application method |
| CN107398307A (en) * | 2016-05-18 | 2017-11-28 | 博奥生物集团有限公司 | A kind of integrated micro-flow control chip |
| CN109142299A (en) * | 2018-09-08 | 2019-01-04 | 重庆科技学院 | A kind of petroleum wastewater heavy metal ion detection chip application method |
| CN109633115A (en) * | 2019-01-08 | 2019-04-16 | 杭州霆科生物科技有限公司 | A kind of water quality comprehensive toxicity detection paper chip |
| CN109752231A (en) * | 2017-11-02 | 2019-05-14 | 浙江清华长三角研究院 | One kind being used for Cr(VI) it is enriched with the micro-fluidic chip of detection |
| CN110508337A (en) * | 2019-03-27 | 2019-11-29 | 广州万孚生物技术股份有限公司 | Vitro detection device and its loading mechanism |
| CN110856821A (en) * | 2018-08-22 | 2020-03-03 | 厦门大学 | Reagent storage module, combination of reagent storage module and communicating vessel and microfluidic chip |
| CN114308144A (en) * | 2020-09-28 | 2022-04-12 | 北京京东方健康科技有限公司 | Micro-fluidic chip |
| CN115561474A (en) * | 2022-12-08 | 2023-01-03 | 深圳市赛特罗生物医疗技术有限公司 | Integrated pesticide residue standardized rapid detection device and method |
| CN115656531A (en) * | 2022-12-08 | 2023-01-31 | 深圳市赛特罗生物医疗技术有限公司 | Integrated pesticide residue standardized rapid detection box and detection method |
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Cited By (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105242053A (en) * | 2015-09-30 | 2016-01-13 | 江苏农林职业技术学院 | Micro-fluidic chip, and intelligent experiment system and method used for micro-fluidic chip |
| CN105353144A (en) * | 2015-10-14 | 2016-02-24 | 江苏理工学院 | Micro-fluidic intelligent experiment apparatus and working method thereof |
| CN107398307B (en) * | 2016-05-18 | 2023-07-25 | 博奥生物集团有限公司 | Integrated micro-fluidic chip |
| CN107398307A (en) * | 2016-05-18 | 2017-11-28 | 博奥生物集团有限公司 | A kind of integrated micro-flow control chip |
| CN105921187A (en) * | 2016-05-23 | 2016-09-07 | 浙江清华长三角研究院 | Micro-fluidic chip for rapid detection of pesticide residues |
| CN106053859A (en) * | 2016-08-02 | 2016-10-26 | 杭州霆科生物科技有限公司 | Centrifugal type glycosylated hemoglobin detection micro-fluidic chip |
| CN106053859B (en) * | 2016-08-02 | 2018-07-10 | 杭州霆科生物科技有限公司 | A kind of centrifugal glycosylated hemoglobin detection micro-fluidic chip |
| CN106119085B (en) * | 2016-08-23 | 2018-12-11 | 闫维新 | A kind of real-time fluorescence PCR mixing microchannel chip |
| CN106119085A (en) * | 2016-08-23 | 2016-11-16 | 闫维新 | A kind of real-time fluorescence PCR mixing microchannel chip |
| CN106525724A (en) * | 2016-10-26 | 2017-03-22 | 杭州霆科生物科技有限公司 | Centrifugal fully integrated detection system of methanal and pH value |
| CN106526178A (en) * | 2016-12-08 | 2017-03-22 | 刘宏川 | Continuous quantitative readable detection device adopting immunochromatography |
| CN107129930B (en) * | 2017-06-09 | 2019-11-26 | 北京百康芯生物科技有限公司 | A kind of fully integrated detection of nucleic acids micro-fluidic chip and its application method |
| CN107129930A (en) * | 2017-06-09 | 2017-09-05 | 北京百康芯生物科技有限公司 | A kind of fully integrated detection of nucleic acids micro-fluidic chip and its application method |
| CN109752231A (en) * | 2017-11-02 | 2019-05-14 | 浙江清华长三角研究院 | One kind being used for Cr(VI) it is enriched with the micro-fluidic chip of detection |
| CN110856821A (en) * | 2018-08-22 | 2020-03-03 | 厦门大学 | Reagent storage module, combination of reagent storage module and communicating vessel and microfluidic chip |
| CN109142299A (en) * | 2018-09-08 | 2019-01-04 | 重庆科技学院 | A kind of petroleum wastewater heavy metal ion detection chip application method |
| CN109142299B (en) * | 2018-09-08 | 2023-07-18 | 重庆科技学院 | Application method of petroleum wastewater heavy metal ion detection chip |
| CN109633115A (en) * | 2019-01-08 | 2019-04-16 | 杭州霆科生物科技有限公司 | A kind of water quality comprehensive toxicity detection paper chip |
| CN110508337A (en) * | 2019-03-27 | 2019-11-29 | 广州万孚生物技术股份有限公司 | Vitro detection device and its loading mechanism |
| CN114308144A (en) * | 2020-09-28 | 2022-04-12 | 北京京东方健康科技有限公司 | Micro-fluidic chip |
| CN115561474A (en) * | 2022-12-08 | 2023-01-03 | 深圳市赛特罗生物医疗技术有限公司 | Integrated pesticide residue standardized rapid detection device and method |
| CN115656531A (en) * | 2022-12-08 | 2023-01-31 | 深圳市赛特罗生物医疗技术有限公司 | Integrated pesticide residue standardized rapid detection box and detection method |
| CN115561474B (en) * | 2022-12-08 | 2024-01-02 | 深圳市赛特罗生物医疗技术有限公司 | Integrated pesticide residue standardized rapid detection device and method |
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