CN103865754A - Microfluidic-based enzyme inhibition reaction platform and analysis method - Google Patents
Microfluidic-based enzyme inhibition reaction platform and analysis method Download PDFInfo
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/16—Microfluidic devices; Capillary tubes
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
Abstract
The invention provides a microfluidic-based enzyme inhibition reaction platform and an analysis method. The negative pressure which is spontaneously generated in a micro-fluidic chip serves as driving force to promote liquid flow to sequentially enter an enzyme inhibition reaction chamber and a detection chamber, and enzyme inhibition reaction and activity analysis are realized. The microfluidic-based enzyme inhibition reaction platform consists of an upper layer and a lower layer, wherein the upper layer is a blank cover plate; the lower layer is a micro-fluidic chip for fixing biochemical reagents; a fluid channel or cavity is formed between the upper and lower layers through sealing; the micro-fluidic chip consists of enzyme inhibition reaction and analysis functional units; and each enzyme inhibition reaction and analysis functional unit consists of a sampling port, an enzyme inhibition reaction chamber, a detection chamber and an air chamber. According to the micro-fluidic chip-based enzyme inhibition reaction analysis method provided in the invention, rapid and accurate field detection of toxic and harmful substances such as heavy metal ions and pesticide residues based on the enzyme inhibition reaction and analysis principle is expected to be realized, and the market prospect is huge.
Description
Technical field
The present invention relates to microfluidic chip analysis field, particularly a kind of enzyme inhibited reaction platform and analytical procedure based on micro-fluidic.
Background technology
Enzyme is to maintain the metabolic important catalyst of organism, some components such as heavy metal ion, the agricultural chemicals etc. that enter in organism all have strong especially avidity with the active centre of some enzyme, there is enzyme inhibited reaction and enzyme is lost activity, there are a series of variations in the neural system of finally bringing out in organism, finally causes organism ill or dead.Based on above-mentioned principle, enzyme inhibited reaction and analytical procedure have extremely deep research and application extremely widely at the detection field of the hazardous and noxious substances such as heavy metal ion, organophosphorus and carbamate chemicals for agriculture.For example, at present, the Fast Determination of Pesticide Residue method and the instrument (mainly comprising the residual tacheometer of agriculture and the residual quick measuring card of agriculture) that suppress method principle based on enzyme have been put into national standard and industry standard (GB/T5009.199-2003), and be widely used in production base, the market of farm produce, supermarket and the superintendent offices such as vegetables, fruit, tealeaves, becoming the mainstream technology of China's Fast Determination of Pesticide Residue, is current and one of the prefered method of Practice for Pesticide Residue in Agricultural Products rapid detection in one period from now on.
But the existing Fast Determination of Pesticide Residue product often biochemical analysis laboratory detection method based on traditional is realized enzyme inhibited reaction analyzing and testing.For example, the residual tacheometer of existing agriculture mainly relies on a large amount of annex such as pipettor, beaker, cuvette and analyzes vessel and completes enzyme inhibition analysis, operation steps comprises that solution preparation (comprising extracting solution, enzyme, developer, substrate), enzyme inhibited reaction, color reaction process are loaded down with trivial details, all dependence manually completes, length consuming time, and operator need to possess certain expertise, can only in laboratory, carry out, be difficult to realize field quick detection; In order to realize field quick detection, people have also developed the residual quick measuring card of agriculture based on enzyme inhibited reaction principle, although this method is simple and easy to do, but there is the shortcomings such as reaction homogeneity is poor, analysis accuracy rate is low in this method, this is because the method relies on the how empty solid dielectric such as film, test paper surface immobilized enzyme, then solution sample drop is added on to test paper surface and completes enzyme inhibited reaction process, the enzyme inhibited reaction that belongs to a kind of " dry chemical method " is analyzed, and is subject to the factor impacts such as the fixing heterogeneity of reagent, solution evaporation larger.
Micro-fluidic chip refers to a kind of biological or chemical laboratory building on the chip of more than square centimeters.It makes the basic operation units such as reaction related in biological and chemical field, separation, cultivation, sorting, detection respectively the member of micro-/ nano magnitude, be integrated on a very little chip, form network by microchannel, run through whole system with controlled fluid, in order to realize the various functions in conventional biological or chemical laboratory.Because micro-fluidic chip technology has, sample size is little, integrated level is high, easily realize automatization control and high throughput analysis, makes to carry out the more conventional analytic sample pre-treatment of biochemical reaction operation more convenient, quick, with low cost on micro-fluidic chip.And what the biochemical reaction of high specific was powerful with micro-fluidic chip separate, detectivity combines and will demonstrate more significant advantage.
Therefore, if can be on micro-fluidic chip technology platform, develop a kind of platform for enzyme inhibited reaction and analytical procedure, not only will make the significantly shortening of sample preparation time, reagent and significantly reduction of instrument cost, detection resolution and sensitivity significantly improve, and the residual quick measuring card technology of enzyme inhibited reaction based on solution reaction contrast agriculture will greatly improve reaction homogeneity and controllability.Particularly biosensor or pocket detecting instrument are further integrated with micro-fluidic chip, will greatly be dwindled the volume of detection system, make the scene based on enzyme inhibited reaction, easy, quick, accurate detection become possibility.
Summary of the invention
The object of the invention is to enzyme inhibited reaction and analytical integration, to micro-fluidic chip platform, to utilize the fluid operated technology of micro-fluidic chip, realize enzyme inhibited reaction fast, evenly, efficiently carry out, finally realize biochemical enzyme analysis fast and accurately.
For achieving the above object, the invention provides a kind of enzyme inhibited reaction platform and analytical procedure based on micro-fluidic, negative pressure using spontaneous generation in micro-fluidic chip impels liquid stream to enter successively enzyme inhibited reaction chamber and sensing chamber as motivating force, completes enzyme inhibited reaction and activation analysis.
The invention provides a kind of enzyme inhibited reaction platform based on micro-fluidic chip, this platform is by upper and lower two-layer composition, and upper strata is blank cover plate, and lower floor is the micro-fluidic chip of fixing biochemical reagents, upper and lower two-layerly forms fluid channel or chamber by sealing-in.
Enzyme inhibited reaction platform based on micro-fluidic chip provided by the invention, described micro-fluidic chip is made up of enzyme inhibited reaction and analytic function unit; Each enzyme inhibited reaction and analytic function unit are made up of injection port, enzyme inhibited reaction chamber, sensing chamber, wind box.
Enzyme inhibited reaction platform based on micro-fluidic chip provided by the invention, the material of described blank cover plate has following characteristics: the material of cover plate can be a kind of or two kinds of combinations during glass, polydimethylsiloxane, polymethylmethacrylate, polycarbonate, polyethylene, polyvinyl chloride, polystyrene, tetrafluoroethylene or cycloolefin analog copolymer gather; At least guarantee that wind box top is elastopolymer thin-film material, specifically comprises polydimethylsiloxane, polyvinyl chloride, polystyrene, tetrafluoroethylene, polyethylene terephthalate; Described elastic thin polymer film thickness is 0.1～1 millimeter, has masterpiece used time generation deformation outside, complete restorable character during without External Force Acting.
Enzyme inhibited reaction platform based on micro-fluidic chip provided by the invention, described wind box is closed cavity body structure, top is elastopolymer film, in the time having pressed by external force Elastic Film, Elastic Film distortion, gas discharging in wind box, the inner normal pressure that produces of micro-fluidic chip; Otherwise in the time that external force is cancelled, Elastic Film will resile, gas backstreaming in wind box, the inner negative pressure that produces of micro-fluidic chip.
Enzyme inhibited reaction platform based on micro-fluidic chip provided by the invention, the material of described micro-fluidic chip is glass, polydimethylsiloxane, polymethylmethacrylate, polycarbonate, polyvinyl chloride, polystyrene, tetrafluoroethylene or cycloolefin analog copolymer.
Enzyme inhibited reaction platform based on micro-fluidic chip provided by the invention, the number of described enzyme inhibited reaction and analytic function unit is 1～100.
Enzyme inhibited reaction platform based on micro-fluidic chip provided by the invention, that the bottom of described enzyme inhibited reaction chamber is fixed with is Powdered, film like or block enzyme reagent; Described fixing means comprises finishing, gel embedding or block encapsulation.Described enzyme comprises acetylcholinesterase, butyrylcholine esterase, than the animal ferments such as sub-enzyme or plant enzyme
Enzyme inhibited reaction platform based on micro-fluidic chip provided by the invention, the bottom of described sensing chamber is fixed with biosensor, specifically comprise that Enzyme sensor, substrate and developer etc. can be used in senser element or the biochemical reagents of discriminance analysis enzymic activity, for example enzyme electrodes.Described substrate and developer preferentially use acetyl cholinesterase iodide, indophenols acetic ester, 5,5 '-dithio two (2-nitrobenzoic acid).
The invention provides a kind of enzyme inhibited reaction analytical procedure based on micro-fluidic chip, the negative pressure providing using wind box impels liquid stream to enter successively enzyme inhibited reaction chamber and sensing chamber as motivating force: first press wind box discharge section or whole air, sample port place is full of to sample solution; After removing the appropriate pressure of wind box, keep constant pressure, the certain negative pressure of the inner generation of micro-fluidic chip, sample solution enters enzyme inhibited reaction chamber under this pressure action; After enzyme inhibited reaction finishes, continue to remove appropriate or whole pressure, the negative pressure of generation impels sample solution to break through valve, enters sensing chamber and complete the analyzing and testing of enzymic activity.
The invention provides a kind of enzyme inhibited reaction analytical procedure based on micro-fluidic chip, described enzyme inhibited reaction can be both static carrying out in reaction chamber, also can be by repeatedly pressing and the Elastic Film of release air chamber, alternately property ground produces normal pressure and negative pressure, thereby impel sample solution to-and-fro movement in enzyme inhibited reaction chamber, realize a kind of dynamic response of reciprocal streaming, can greatly improve reaction efficiency and homogeneity.
The invention provides a kind of enzyme inhibited reaction analytical procedure based on micro-fluidic chip, in described sensing chamber, the analysing and detecting method of enzymic activity comprises Electrochemical Detection and optical detection, can coordinate portable Electrochemical Detection instrument or optical detector to complete the analysis of enzyme inhibited reaction and measure.
The invention has the advantages that: 1) at the inner immobilized enzyme reagent of micro-fluidic chip and biosensor, without using remaining annex and avoiding a large amount of loaded down with trivial details solution preparation work; 2) utilize the wind box of particular design in micro-fluidic chip that a kind of easy flow injecting method is provided, automatically complete enzyme inhibited reaction; Further combined with reversingcurrent control, improve enzyme inhibited reaction homogeneity and reaction efficiency thereby can significantly accelerate material mass transfer simultaneously, shorten the enzyme inhibited reaction time; 3) reagent and sample consumption are reduced to microlitre or even receive upgrading not from traditional milliliter rank, significantly reduce testing cost; 4) can carry out parallel analysis to multiple samples simultaneously; 5) easy and simple to handle, non-specialised staff also can carry out scene, Fast Measurement.
Enzyme inhibited reaction analytical procedure based on micro-fluidic chip provided by the present invention, is expected to realize the scene of the hazardous and noxious substances such as heavy metal ion, pesticide residue based on enzyme inhibited reaction and analysis principle, fast, accurately detect, and market outlook are huge.
Accompanying drawing explanation
Fig. 1. (1) a kind of enzyme inhibited reaction and analysis platform schematic diagram based on micro-fluidic chip, wherein, A is cover plate, B is the micro-fluidic chip of fixing biochemical reagents; (2) single enzyme inhibited reaction and analytic function unit composition schematic diagram, wherein, a is injection port, and b is enzyme inhibited reaction chamber, and c is sensing chamber, and d is wind box.
Fig. 2. a kind of micro-fluidic static enzym inhibited reaction and analysis process schematic diagram based on single wind box.(1) press wind box, form negative pressure; (2) discharge appropriate pressure, Ngatively pressurized sampling, solution enters to enzyme inhibited reaction chamber from sample port; (3) after enzyme inhibited reaction finishes, continue to discharge whole pressure, negative pressure control solution enters sensing chamber, realizes enzyme activity assay and detects.
Fig. 3. a kind of micro-fluidic static enzym inhibited reaction and analysis process schematic diagram based on two wind boxs.(1) press two wind boxs, form negative pressure; (2) discharge a wind box, Ngatively pressurized sampling, solution enters to enzyme inhibited reaction chamber from sample port; (3) after enzyme inhibited reaction finishes, discharge another wind box, negative pressure control solution enters sensing chamber, realizes enzyme activity assay and detects.
Fig. 4. based on micro-fluidic enzyme inhibited reaction and the analysis process schematic diagram of reversingcurrent dynamic response pattern.(1) press wind box, form negative pressure; (2) discharge appropriate pressure, Ngatively pressurized sampling, solution enters to enzyme inhibited reaction chamber from sample port; (3) continue to press wind box, form normal pressure, solution moves to sample port from enzyme inhibited reaction chamber; (4) repeat 2,3 steps, the enzyme of realizing based on reversingcurrent suppresses dynamic response; (5) after enzyme inhibited reaction finishes, discharge whole pressure, negative pressure control solution enters sensing chamber, realizes enzyme activity assay and detects.
Specific embodiments
The following examples will be further described the present invention, but not thereby limiting the invention.
1 one kinds of embodiment micro-fluidic static enzym inhibited reaction platform and the analytical procedure based on single wind box
As shown in Figure 1, based on micro-fluidic enzyme inhibited reaction and the analysis platform of single wind box, this platform is by upper and lower two-layer composition, the blank cover plate that A is upper strata, B is the micro-fluidic chip of the fixing biochemical reagents of lower floor, upper and lower two-layerly forms fluid channel or chamber by sealing-in; Micro-fluidic chip is made up of enzyme inhibited reaction and analytic function unit; Each enzyme inhibited reaction and analytic function unit are made up of injection port a, enzyme inhibited reaction chamber b, the c of sensing chamber, wind box d.Wherein, wind box d is closed cavity body structure, and top is elastopolymer film, in the time having pressed by external force Elastic Film, and Elastic Film distortion, gas discharging in wind box, the inner normal pressure that produces of micro-fluidic chip; Otherwise in the time that external force is cancelled, Elastic Film will resile, gas backstreaming in wind box, the inner negative pressure that produces of micro-fluidic chip.Rely on above-mentioned normal pressure and negative pressure cavity, can realize the operation of micro-fluidic chip internal solution, thereby realize the regulation and control of enzyme inhibited reaction.
More specifically, a kind of micro-fluidic static enzym inhibited reaction based on single wind box and analysis process are as shown in Figure 2: (1) presses the Elastic Film at wind box top, the air in exhausted air chamber, the inner negative pressure that forms of micro-fluidic chip; (2) injection port of micro-fluidic chip is submerged in sample solution, or sample solution is added in injection port, then after the Elastic Film at wind box top being discharged to appropriate pressure, keep constant voltage, form Ngatively pressurized sampling, solution enters to enzyme inhibited reaction chamber from sample port; (3) maintain wind box top pressure, after enzyme inhibited reaction finishes (about 3-10min), further continue whole pressure at top, release air chamber, the negative pressure control solution producing enters sensing chamber, finally in sensing chamber, utilize electrochemical analysis or optical analysis principle to realize enzyme activity assay and detect, thereby obtain the measurement result of heavy metal in sample or pesticide residue.
2 one kinds of embodiment micro-fluidic static enzym inhibited reaction platform and the analytical procedure based on two wind boxs
Micro-fluidic static enzym inhibited reaction based on two wind boxs and analysis process be as shown in Figure 3: (1) presses the Elastic Film at two wind box tops, the air in exhausted air chamber, the inner negative pressure that forms of micro-fluidic chip; (2) injection port of micro-fluidic chip is submerged in sample solution, or sample solution is added in injection port, maintain another wind box top pressure, the Elastic Film pressure at one of them wind box top is discharged completely simultaneously, form Ngatively pressurized sampling, solution enters to enzyme inhibited reaction chamber from sample port; (3) after enzyme inhibited reaction finishes (about 3-10min), further discharge whole pressure at another wind box top, the negative pressure control solution producing enters sensing chamber, finally in sensing chamber, utilize electrochemical analysis or optical analysis principle to realize enzyme activity assay and detect, thereby obtain the measurement result of heavy metal in sample or pesticide residue.
3 one kinds of embodiment micro-fluidic enzyme inhibited reaction platform and the analytical procedure based on reversingcurrent dynamic response pattern
In the present embodiment, a kind of micro-fluidic enzyme inhibited reaction platform based on reversingcurrent dynamic response pattern and analytical procedure be as shown in Figure 4: (1) presses the Elastic Film at wind box top, air in exhausted air chamber, the inner negative pressure that forms of micro-fluidic chip; (2) injection port of micro-fluidic chip is submerged in sample solution, or sample solution is added in injection port, then after the Elastic Film at wind box top being discharged to appropriate pressure, keep constant voltage, form Ngatively pressurized sampling, solution enters to enzyme inhibited reaction chamber from sample port; (3) continue to press wind box, form normal pressure, under this pressure action, solution will move to sample port from enzyme inhibited reaction chamber; (4) repeat 2,3 steps, sample solution will back and forth flow in enzyme inhibited reaction chamber, and the enzyme of realizing based on reversingcurrent suppresses dynamic response; (5) after enzyme inhibited reaction finishes, discharge whole pressure, negative pressure control solution enters sensing chamber, realizes enzyme activity assay and detects.
Comparative example 1 and embodiment 2, use micro-fluidic enzyme inhibited reaction platform and the analytical procedure based on reversingcurrent dynamic response pattern described in the present embodiment, the reversingcurrent dynamic response of enzyme inhibited reaction be can realize, thereby homogeneity and the speed of reaction of enzyme inhibited reaction improved significantly.
Use enzyme inhibited reaction and the analytical procedure based on micro-fluidic chip provided by the present invention, be expected to realize the scene of the hazardous and noxious substances such as heavy metal ion, pesticide residue based on enzyme inhibited reaction and analysis principle, fast, accurately detect, market outlook are huge.
Claims (10)
1. the enzyme inhibited reaction platform based on micro-fluidic, is characterized in that: this platform is by upper and lower two-layer composition, and upper strata is blank cover plate, and lower floor is the micro-fluidic chip of fixing biochemical reagents, upper and lower two-layerly forms fluid channel or chamber by sealing-in; Described micro-fluidic chip is made up of enzyme inhibited reaction and analytic function unit; Each enzyme inhibited reaction and analytic function unit are made up of injection port, enzyme inhibited reaction chamber, sensing chamber, wind box.
2. according to the enzyme inhibited reaction platform based on micro-fluidic described in claim 1, it is characterized in that: the material of described blank cover plate has following characteristics: the material of cover plate can be a kind of or two kinds of combinations during glass, polydimethylsiloxane, polymethylmethacrylate, polycarbonate, polyethylene, polyvinyl chloride, polystyrene, tetrafluoroethylene or cycloolefin analog copolymer gather; At least guarantee that wind box top is elastopolymer thin-film material, specifically comprises polydimethylsiloxane, polyvinyl chloride, polystyrene, tetrafluoroethylene, polyethylene terephthalate; Described elastic thin polymer film thickness is 0.1～1 millimeter, has masterpiece used time generation deformation outside, complete restorable character during without External Force Acting.
3. according to the enzyme inhibited reaction platform based on micro-fluidic described in claim 1 and 2, it is characterized in that: described wind box is closed cavity body structure, top is elastopolymer film, in the time having pressed by external force Elastic Film, Elastic Film distortion, gas discharging in wind box, the inner normal pressure that produces of micro-fluidic chip; Otherwise in the time that external force is cancelled, Elastic Film will resile, gas backstreaming in wind box, the inner negative pressure that produces of micro-fluidic chip.
4. according to the enzyme inhibited reaction platform based on micro-fluidic described in claim 1, it is characterized in that: the material of described micro-fluidic chip is glass, polydimethylsiloxane, polymethylmethacrylate, polycarbonate, polyvinyl chloride, polystyrene, tetrafluoroethylene or cycloolefin analog copolymer.
5. according to the enzyme inhibited reaction platform based on micro-fluidic described in claim 1, it is characterized in that: the number of described enzyme inhibited reaction and analytic function unit is 1～100.
6. according to the enzyme inhibited reaction platform based on micro-fluidic described in claim 1, it is characterized in that: the bottom of described enzyme inhibited reaction chamber is fixed with Powdered, film like or block enzyme reagent; Described fixing means comprises finishing, gel embedding or block encapsulation.
7. according to the enzyme inhibited reaction platform based on micro-fluidic described in claim 1, it is characterized in that: the bottom of described sensing chamber is fixed with biosensor, specifically comprise that Enzyme sensor, developer etc. can be used in senser element or the reagent of discriminance analysis enzymic activity, for example enzyme electrodes.
8. according to the enzyme inhibition analysis method based on micro-fluidic described in claim 1, it is characterized in that: the negative pressure providing using wind box impels liquid stream to enter successively enzyme inhibited reaction chamber and sensing chamber as motivating force: first press wind box discharge section or whole air, sample port place is full of to sample solution; After removing the appropriate pressure of wind box, keep constant pressure, the certain negative pressure of the inner generation of micro-fluidic chip, sample solution enters enzyme inhibited reaction chamber under this pressure action; After enzyme inhibited reaction finishes, continue to remove appropriate or whole pressure, the negative pressure of generation impels sample solution to break through valve, enters sensing chamber and complete the analyzing and testing of enzymic activity.
9. according to the enzyme inhibition analysis method based on micro-fluidic described in claim 1 and 9, it is characterized in that: described enzyme inhibited reaction can be both static reaction in reaction chamber, also can be by repeatedly pressing and the Elastic Film of release air chamber, alternately property ground produces normal pressure and negative pressure, thereby impel sample solution to-and-fro movement in enzyme inhibited reaction chamber, realize a kind of reversingcurrent dynamic response, can greatly improve reaction efficiency and homogeneity.
10. according to the enzyme inhibition analysis method based on micro-fluidic described in claim 1 and 9, it is characterized in that: in described sensing chamber, the analysing and detecting method of enzymic activity comprises Electrochemical Detection and optical detection.
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| CN104849477A (en) * | 2015-01-23 | 2015-08-19 | 江苏大学 | Portable microfluidic detection apparatus and method for organophosphorus pesticide |
| CN105115911A (en) * | 2015-09-18 | 2015-12-02 | 清华大学 | Water quality detecting method and micro-fluidic chip special for water quality detecting method |
| CN105921187A (en) * | 2016-05-23 | 2016-09-07 | 浙江清华长三角研究院 | Micro-fluidic chip for rapid detection of pesticide residues |
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| CN105964315A (en) * | 2016-05-23 | 2016-09-28 | 杭州霆科生物科技有限公司 | Multi-stage self-control micro-fluidic chip |
| CN106940351A (en) * | 2017-03-01 | 2017-07-11 | 浙江大学 | A kind of method of detection traditional Chinese medicine quality and its bioactive ingredients based on micro-fluidic chip |
| CN108872081A (en) * | 2018-09-04 | 2018-11-23 | 重庆科技学院 | A kind of multi-layer micro-fluidic chips detecting heavy metal ion |
| CN108872081B (en) * | 2018-09-04 | 2023-06-23 | 重庆科技学院 | Multilayer microfluidic chip for detecting heavy metal ions |
| CN109499634A (en) * | 2018-12-13 | 2019-03-22 | 迪亚莱博（张家港）生物科技有限公司 | A kind of micro-fluidic chip and preparation method thereof and detection method |
| CN109499633A (en) * | 2018-12-13 | 2019-03-22 | 迪亚莱博（张家港）生物科技有限公司 | Other diagnosis micro-fluidic chip of bed and preparation method thereof and detection method |
| CN109499634B (en) * | 2018-12-13 | 2023-11-03 | 迪亚莱博（张家港）生物科技有限公司 | Microfluidic chip and preparation method and detection method thereof |
| CN109499633B (en) * | 2018-12-13 | 2024-02-09 | 迪亚莱博（张家港）生物科技有限公司 | Bedside diagnosis micro-fluidic chip and preparation method and detection method thereof |
| CN110343604A (en) * | 2019-07-15 | 2019-10-18 | 浙江大学 | A kind of residual detection chip of micro-fluidic agriculture and application |
| WO2021215996A1 (en) * | 2020-04-24 | 2021-10-28 | Lauschke Volker Martin | Device |
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