CN205056043U - A disc micro -fluidic chip for in batches quick examination of pesticide residue - Google Patents

Abstract

The utility model discloses a disc micro -fluidic chip for in batches quick examination of pesticide residue, a serial communication port, including draw -in groove and two at least detecting element, single detecting element includes that introduction port, fluid passage, enzyme repression reaction tank, color development detect pond, waste liquid pool, air vent, save biochemical reagents in the enzyme repression reaction tank, save the substrate in the color development detects the pond, examining the time measuring chip and putting into the centrifugal separator platform, add the chip with quantitative need checking sample extract from the introduction port, centrifugal force drive solution is flowed through the enzyme repression reaction tank in proper order and is detected the pond with the color development along advancing kind passageway, accomplishes solution phase's enzyme repression reaction and color development reaction respectively, measures the agricultural incomplete inhibiting rate of solution absorbance value calculation at last.

Description

A kind of disk micro-fluidic chip for residues of pesticides batch rapid screening

Technical field

The invention relates to a kind of disk micro-fluidic chip for residues of pesticides batch rapid screening, belongs to micro fluidic chip technical field.

Background technology

Microfluidic chip analysis technology, because having microminiaturization, integrated, the remarkable advantage such as high flux, low cost, is applied to the quick detection of Organic pesticide residues.Chinese patent CN102784617A adopts centrifugal force controlled fluid to flow in chip, completes the detection of residues of pesticides.This chip needs to bury the capillary covering different-diameter and coordinates corresponding centrifuge speed, adds preparation difficulty and the production cost of chip; Packaged chip again after simultaneously need adding reagent when residual detections of agriculture, complex steps, can not meet the demand of the residual speed survey of agriculture.The purpose of this utility model is to provide a kind of disk micro-fluidic chip for residues of pesticides batch rapid screening, all kinds of reaction reagent has prestored and has been stored among chip, structure is simple, cost is low, is beneficial to batch production, is applicable to the batch rapid screening of residues of pesticides.

Utility model content

The technical solution of the utility model is: micro-fluidic chip, it is characterized in that, comprises draw-in groove and at least two detecting units;

Described detecting unit comprises injection port, fluid passage, enzyme level reaction tank, color developing detection pond, waste liquid pool, passage, the biochemical reagents be stored in enzyme level reaction tank, the substrate be stored in color developing detection pond;

Described enzyme level reaction tank, to be sequentially connected in series by fluid passage between color developing detection pond and waste liquid pool, described draw-in groove is positioned at the circle centre position of disk micro-fluidic chip, described injection port is positioned at the position near the center of circle, and described passage is positioned at waste liquid pool end, is communicated with waste liquid pool.

During detection, chip puts into centrifuge platform, quantitative testing sample extract is added chip from injection port, centrifugal force drives solution to flow through enzyme level reaction tank and color developing detection pond successively along sample intake passage, complete enzyme level reaction and the chromogenic reaction of solution phase respectively, finally measure solution absorbance value and calculate the residual inhibiting rate of agriculture.

The utility model has the advantages that: the utility model simplicity of design, cost are low, are beneficial to batch production, chip internal pre-stored reagent, without the need to situ configuration solution, can be used for the batch rapid screening of residues of pesticides.

Accompanying drawing explanation

Fig. 1 is the disk microfluidic chip structure schematic diagram of an embodiment;

Fig. 2 is the structural representation of chip detection unit.

Detailed description of the invention

Below in conjunction with accompanying drawing, the utility model is described further:

With reference to Fig. 1, for the disk micro-fluidic chip of residues of pesticides batch examination, it is characterized in that, comprise draw-in groove (10) and detecting unit.Detecting unit can be two, three or more, specifically in the present embodiment, detecting unit is 12.

With reference to Fig. 2, the structural representation of chip detection unit, comprises injection port (1), fluid passage (2), enzyme level reaction tank (3), color developing detection pond (4), waste liquid pool (5), passage (6), the biochemical reagents (7) be stored in enzyme level reaction tank, the substrate (8) be stored in detection cell.During detection, chip puts into centrifuge platform, quantitative testing sample extract is added chip from injection port, centrifugal force drives solution to flow through enzyme level reaction tank and color developing detection pond successively along sample intake passage, complete enzyme level reaction and the chromogenic reaction of solution phase respectively, finally measure solution absorbance value and calculate the residual inhibiting rate of agriculture.

Claims (1)

1., for a disk micro-fluidic chip for residues of pesticides batch rapid screening, it is characterized in that, comprise draw-in groove (10) and at least two detecting units;

Described detecting unit comprises injection port (1), fluid passage (2), enzyme level reaction tank (3), color developing detection pond (4), waste liquid pool (5), passage (6), the biochemical reagents (7) be stored in enzyme level reaction tank, the substrate (8) be stored in detection cell;

Described enzyme level reaction tank, to be sequentially connected in series by fluid passage between color developing detection pond and waste liquid pool, described draw-in groove is positioned at the circle centre position of disk micro-fluidic chip, described injection port is positioned at the position near the center of circle, and described passage is positioned at waste liquid pool end, is communicated with waste liquid pool.