CN107102000B - Nucleic acid detection device - Google Patents
Nucleic acid detection device Download PDFInfo
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- CN107102000B CN107102000B CN201710395680.5A CN201710395680A CN107102000B CN 107102000 B CN107102000 B CN 107102000B CN 201710395680 A CN201710395680 A CN 201710395680A CN 107102000 B CN107102000 B CN 107102000B
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- nucleic acid
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- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 34
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 34
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 33
- 238000001514 detection method Methods 0.000 title claims abstract description 24
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 73
- 239000007788 liquid Substances 0.000 claims abstract description 62
- 238000012360 testing method Methods 0.000 claims abstract description 49
- 238000007789 sealing Methods 0.000 claims description 8
- 238000000926 separation method Methods 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 4
- 239000012780 transparent material Substances 0.000 claims description 3
- 230000003321 amplification Effects 0.000 abstract description 3
- 238000003199 nucleic acid amplification method Methods 0.000 abstract description 3
- 238000004587 chromatography analysis Methods 0.000 abstract description 2
- 238000012123 point-of-care testing Methods 0.000 description 6
- 238000011161 development Methods 0.000 description 4
- 125000006850 spacer group Chemical group 0.000 description 4
- 230000007613 environmental effect Effects 0.000 description 2
- 239000007769 metal material Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000011330 nucleic acid test Methods 0.000 description 2
- 239000000443 aerosol Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011841 epidemiological investigation Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a nucleic acid detection device which comprises a bottle cap 1, a fixed liquid storage tank 2, test paper 3, a standard sample tube 4, a reaction reagent tube 5, a puncture nail 6 and liquid guide paper 7. The device can realize the disposable rapid detection of various nucleic acid amplified, is more convenient and faster to operate than the traditional nucleic acid amplified chromatography detection, has good tightness and can effectively prevent the pollution of amplified products to the environment. The device reduces the hardness requirement of the existing nucleic acid amplification detection method on the experimental field, simplifies the operation difficulty and the technical level requirement on operators, and can realize the nucleic acid detection in the field or a non-professional laboratory.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a nucleic acid detection device.
Background
Point-of-care testing (POCT) primarily refers to rapid testing performed off-site from the laboratory. In recent years, point of care testing (POCT) has become one of the fastest growing areas of current inspection medicine, and the need for faster, cheaper, technologies, instruments and devices that provide more reliable results has driven the continued development of this market.
The on-site instant detection of the nucleic acid has good application prospect for epidemic prevention and control and epidemiological investigation, and is also very suitable for the fields of biological anti-terrorism, agriculture, food safety and the like, so the on-site instant detection of the nucleic acid develops rapidly. Nucleic acid POCT has achieved integration of a multi-step nucleic acid detection method into a system where the user only needs to add a sample to the system, and the remaining steps are all automatically performed by the system. Nucleic acid POCT adopts a closed disposable detection card or detection tube, so that cross and carrying pollution are well controlled. However, these test cards or test tubes often detect only one nucleic acid at a time, and therefore require a large number of tools, which is inconvenient for field operation.
Disclosure of Invention
Aiming at the problems, the invention aims to provide a nucleic acid detection device which can detect various nucleic acids at one time, is convenient to operate, has good sealing performance and does not pollute the outside.
The technical scheme of the invention is as follows: a nucleic acid detection device comprises a bottle cap 1, a fixed liquid storage tank 2, test paper 3, a standard sample tube 4, a reaction reagent tube 5, a puncture nail 6 and liquid guide paper 7; wherein the fixed liquid storage tank is provided with a preassembled reagent tank 21, a supporting rod 22 and a sample fixing clamping groove 23 for placing a standard sample tube 4; wherein the reaction reagent tube 5 is provided with a test paper fixing groove 51, a middle liquid separation plate 52, a liquid guide paper fixing groove 53 and a puncture nail fixing groove 54; the test paper 3, the puncture nails 6 and the liquid guide paper 7 can be placed in the corresponding fixing grooves of the reaction reagent tube 5; the fixed liquid storage tank (2) can be accommodated in the reaction reagent tube (5), and the fixed liquid storage tank is covered with the bottle cap (1) to form a closed whole.
Wherein, the bottle cap 1 is provided with a sealing part 11 inside. When the detection device is used, once the bottle cap is screwed, the detection device cannot be easily opened, and pollution caused by leakage of nucleic acid amplification products is avoided.
Wherein the positions of the puncture nail fixing grooves 54 respectively correspond to the positions of the pre-filling reagent groove 21 and the sample fixing clamping groove 23. When the sample fixing device is used, the fixing liquid storage tank is screwed into the reaction reagent tube, and the standard sample tubes in the preassembled reagent tank and the sample fixing clamping tank can be respectively pierced by piercing nails at corresponding positions.
Wherein, after the standard sample tube 4 is clamped into the sample fixing clamping groove 23 of the fixing liquid storage groove, the bottom of the standard sample tube is lower than the bottom of the pre-filling reagent groove 21. That is, the tail of the standard sample tube is closer to the reagent tube 5 than the tail of the pre-filled reagent tank is to the reagent tube 5 after being fixed to the sample fixing groove, so that the standard sample tube is contacted with the puncture nail and is punctured by the puncture nail during screwing.
Wherein, in the fixed liquid storage tank 2, the height of the supporting rod 22 is higher than that of the pre-filling reagent tank 21.
Wherein the piercing pin 6 is made of a metallic material.
Wherein the pre-filled reagent vessel 21 and the standard sample tube 4 are made of a material that can be pierced by a piercing pin.
Wherein the number of lances is the sum of the number of standard sample tubes and pre-filled reagent tanks.
In specific implementation, the bottle cap 1 of the testing device is opened, the fixed liquid storage tank 2 is taken out, the prepared reagent is filled into the pre-filled reagent tank 21, and the standard sample tube 4 filled with the standard sample is clamped into the standard sample clamping groove 23; fixing the test paper 3 in a test paper fixing groove 51 of the reaction reagent tube 5, and fixing the liquid guide paper 7 in a liquid guide paper fixing groove 53; then, the fixed liquid storage tank 2 with the standard sample is placed into the reaction reagent tube 5, the bottle cap 1 is slowly screwed on the reaction reagent tube 5, the bottle cap 1 props against the support rod 22 to push the fixed liquid storage tank 2 into the reaction reagent tube 5 due to the fact that the height of the support rod 22 is higher than that of the preassembling reagent tank 21, and the standard sample tube 4 is firstly punctured under the action of the puncture nail 6 and the downward rotation force of the bottle cap 1 due to the fact that the bottom of the standard sample tube 4 is lower than that of the preassembling reagent tank 21, and the sample in the standard sample tube flows to the liquid guide paper 7 along the puncture nail 6; then, the pre-filled reagent tank 21 is also broken by the puncture nails 6 at the corresponding positions, and the reagent in the pre-filled reagent tank flows to the liquid guide paper 7 as well, is mixed and reacts with the sample, and simultaneously is guided to the test paper 3 under the action of the liquid guide paper; finally, the reaction of the test paper is observed to obtain a required test result; the whole reaction reagent tube 5 is transparent or transparent material is arranged at the test paper 3, so that an operator can observe the color development result through the transparent tube wall without opening the device and judge whether the nucleic acid to be detected exists.
The reagent tube 5 is provided with a middle spacer plate 52, the middle spacer plate 52 divides the internal space of the reagent tube into two halves, and each side space is provided with a test paper fixing groove 51, a liquid guide paper fixing groove 53 and a puncture nail fixing groove 54, respectively. The liquid confluence on two sides can be prevented by the middle liquid separation plate, so that the spaces on two sides of the middle liquid separation plate are respectively a reaction reagent space, the reaction is carried out without mutual influence, and the disposable detection of various nucleic acids can be realized.
Further, the intermediate liquid-barrier may be provided in two or more.
The middle spacer liquid plate is arranged in the reaction reagent tube, so that two (or more) nucleic acid amplification products can be led through liquid guide paper, chromatography is respectively carried out on two (or more) test papers, and color development is carried out through immune reaction, thus multiple nucleic acid results can be detected at one time, and the detection efficiency is greatly improved.
The beneficial effects of this device include:
(1) The nucleic acid testing device simplifies the operation flow of operators, and can complete the testing work without professional experimenters;
(2) The device is provided with the pre-filling reagent tank, so that the reaction reagent can be pre-filled, and the probability of false positive in the experimental process is greatly reduced;
(3) The standard sample fixing clamping groove is arranged in the fixing liquid storage groove, so that the installation is simple and convenient;
(4) The bottle cap of the testing device is provided with a sealing component, so that the sealing component can play a role in sealing during the reaction, and the aerosol in the testing device is prevented from evaporating, so that the pollution of reagents and nucleic acid to the environment during the test can be avoided;
(5) The reaction reagent tube is provided with a liquid guide paper fixing groove, so that the directional flow of liquid is ensured, and the sensitivity and accuracy of the test are improved;
(6) Multiple pre-filled reagent reservoirs and standard sample tubes may be provided and multiple or multicomponent nucleic acids may be tested at one time.
The device is a rapid detection device for nucleic acid, compared with the prior market testing device, can realize rapid detection of various nucleic acids at one time, has more convenient operation, good tightness, small pollution to the outside, eliminates environmental interference, reduces the testing cost, realizes application in a field or non-professional laboratory, highly simplifies the operation difficulty and the technical level requirement on operators, and realizes operation foolproof. In addition, the device has low cost and can meet the requirement of a wider nucleic acid test market.
Drawings
FIG. 1 is a schematic diagram showing the components of a nucleic acid detecting apparatus according to example 1. Wherein, 1, bottle cap; 2. a fixed liquid storage tank; 3. test paper; 4. a standard sample tube; 5. a reagent tube; 6. puncturing the nail; 7. and (5) liquid guiding paper.
Fig. 2 is a cross-sectional view of a bottle cap of the testing device. Wherein, 11, sealing part; 12. bottle cap threads.
FIG. 3 is a schematic illustration of a fixed reservoir configuration. Wherein, 21, preassemble the reagent tank; 22. a support rod; 23. sample fixing clamping groove.
FIG. 4 is a schematic cross-sectional view of a reagent tube. 51, a test paper fixing groove; 52. a middle spacer fluid plate; 53. a liquid guiding paper fixing groove; 54. the nail fixing groove is pierced.
Detailed Description
The invention will be further illustrated with reference to specific examples. It is to be understood that these examples are illustrative of the present invention and are not intended to limit the scope of the present invention. Further, it is understood that various changes and modifications of the invention may be made by those skilled in the art after reading the disclosure of the invention, and such equivalents are intended to fall within the scope of the invention as defined by the claims.
In the case of example 1,
taking fig. 1 as an example, the device for arranging 2 pre-assembled reagent tanks and 2 standard sample tubes is composed of a test tube bottle cap 1, a fixed liquid storage tank 2, test paper 3, a standard sample tube 4, a reaction reagent tube 5, a puncture nail 6 and liquid guide paper 7; as shown in fig. 3, the fixed liquid storage tank is provided with a pre-filling reagent tank 21, a supporting rod 22 and a sample fixing clamping groove 23; as shown in fig. 4, the reaction reagent tube 5 is provided with a test paper fixing groove 51, a middle liquid separation plate 52, a liquid guide paper fixing groove 53 and a puncture nail fixing groove 54; two puncture nail fixing grooves are respectively formed in two sides of the middle liquid separation plate; the test paper 3 can be placed in the test paper fixing groove 51, the puncture nail 6 can be placed in the puncture nail fixing groove 54, and the liquid guide paper 7 can be placed in the liquid guide paper fixing groove 53; as shown in fig. 2, the bottle cap 1 is provided with a sealing member 11 inside the bottle cap. After the standard sample tube is clamped into the sample fixing clamping groove of the fixing liquid storage groove, the bottom of the standard sample tube is lower than the bottom of the pre-loading reagent groove. In the fixed liquid storage tank, the height of the supporting rod is higher than that of the pre-filling reagent tank.
The piercing pin is made of a metallic material. The pre-filled reagent tank and the standard sample tube are made of materials which can be punctured by a puncture nail, such as PE or PP materials.
The positions of the puncture nail fixing grooves correspond to the positions of the pre-loading reagent grooves and the sample fixing clamping grooves. That is, the lancet in the lancet holding slot can pierce the standard sample tube in the pre-filled reagent slot, sample holding card slot, from the bottom. The number of piercing pins is the sum of the number of pre-filled reagent reservoirs and standard sample tubes.
In the specific implementation, the testing device is opened, the fixed liquid storage tank 2 is taken out, the prepared reagent is filled into the pre-filling reagent tank 21, and the standard sample tube 4 filled with the standard sample is clamped into the standard sample clamping groove 23; fixing the test paper 3 in a test paper fixing groove 51 of the reaction reagent tube 5, and fixing the liquid guide paper 7 in a liquid guide paper fixing groove 53; then the fixed liquid storage tank 2 with the standard sample is placed into the reaction reagent tube 5, the bottle cap 1 is slowly screwed on the reaction reagent tube 5, the bottle cap 1 props against the supporting rod 22 to push the fixed liquid storage tank 2 into the reaction reagent tube 5, and as the bottom of the standard sample tube is lower than the bottom of the preassembled reagent tank, the standard sample tube 4 is pierced under the action of the piercing pin 6 and the downward rotation force of the bottle cap, and the sample in the standard sample tube flows to the liquid guide paper 7 along the piercing pin 6; then, the pre-filled reagent tank 21 is also broken by the puncture nails 6 at the corresponding positions, and the reagent in the pre-filled reagent tank flows to the liquid guide paper 7 as well, is mixed and reacts with the sample, and simultaneously is guided to the test paper 3 under the action of the liquid guide paper; finally, the reaction of the test paper is observed to obtain a required test result; the whole reaction reagent tube 5 is transparent or transparent material is arranged at the test paper 3, so that an operator can observe the color development result through the transparent tube wall without opening the device and judge whether the nucleic acid to be detected exists.
In actual production and testing, the number of the pre-assembled reagent tanks and the standard sample tubes can be determined as required, but is not limited to 1 or 2, and the corresponding piercing nails, liquid guide paper and test paper are provided.
The device is a rapid detection device for nucleic acid, compared with the prior market testing device, can realize rapid detection of various nucleic acids at one time, has more convenient operation, good tightness, small pollution to the outside, eliminates environmental interference, reduces the testing cost, realizes application in a field or non-professional laboratory, highly simplifies the operation difficulty and the technical level requirement on operators, and realizes operation foolproof. In addition, the device has low cost and can meet the requirement of a wider nucleic acid test market.
The foregoing is merely a preferred embodiment of the present invention, and it should be noted that modifications and improvements could be made by those skilled in the art without departing from the inventive concept, which falls within the scope of the present invention.
Claims (3)
1. The nucleic acid detection device is characterized by comprising a bottle cap (1), a fixed liquid storage tank (2), test paper (3), a standard sample tube (4), a reaction reagent tube (5), a puncture nail (6) and liquid guide paper (7);
wherein the fixed liquid storage tank is provided with a preassembled reagent tank (21), a supporting rod (22) and a sample fixing clamping groove (23) for placing a standard sample tube (4);
wherein the reaction reagent tube (5) is provided with a test paper fixing groove (51), a middle liquid separation plate (52), a liquid guide paper fixing groove (53) and a puncture nail fixing groove (54); the test paper (3), the puncture nails (6) and the liquid guide paper (7) can be placed in corresponding fixing grooves of the reaction reagent tube (5);
the fixed liquid storage tank (2) can be accommodated in the reaction reagent tube (5), and the fixed liquid storage tank is covered with the bottle cap (1) to form a closed whole;
the bottle cap (1) is internally provided with a sealing part (11);
the positions of the puncture nail fixing grooves (54) respectively correspond to the positions of the pre-loading reagent groove (21) and the sample fixing clamping groove (23);
after the standard sample tube (4) is clamped into a sample fixing clamping groove (23) of the fixed liquid storage groove (2), the bottom of the standard sample tube is lower than the bottom of the preassembled reagent groove (21);
in the fixed liquid storage tank (2), the height of the supporting rod (22) is higher than that of the pre-filling reagent tank (21);
the whole reaction reagent tube (5) is transparent or transparent material is arranged at the test paper (3).
2. The nucleic acid testing device of claim 1, wherein the pre-filled reagent reservoir and the standard sample tube are made of a material that is penetrable by a spike.
3. The nucleic acid testing device of claim 1, wherein the number of lances is the sum of the number of standard sample tubes and pre-filled reagent tanks.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710395680.5A CN107102000B (en) | 2017-05-31 | 2017-05-31 | Nucleic acid detection device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710395680.5A CN107102000B (en) | 2017-05-31 | 2017-05-31 | Nucleic acid detection device |
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| Publication Number | Publication Date |
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| CN107102000A CN107102000A (en) | 2017-08-29 |
| CN107102000B true CN107102000B (en) | 2024-03-08 |
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| CN201710395680.5A Active CN107102000B (en) | 2017-05-31 | 2017-05-31 | Nucleic acid detection device |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111876317A (en) * | 2020-07-07 | 2020-11-03 | 上海市浦东新区人民医院 | Nucleic acid amplification detection device |
| CN114058472A (en) * | 2020-07-30 | 2022-02-18 | 苏州中加康美科技有限公司 | Nucleic acid reagent reaction unit |
| CN112345516A (en) * | 2020-11-16 | 2021-02-09 | 苏州瀚诺馨生物科技有限公司 | Infectious disease detection box |
| CN116496878A (en) * | 2022-01-19 | 2023-07-28 | 江苏为真生物医药技术股份有限公司 | Sealing device applied to rapid detection and use method and application thereof |
| CN118311245B (en) * | 2024-06-11 | 2024-10-15 | 连云港市质量技术综合检验检测中心 | Nucleic acid test paper detection device and detection method thereof |
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| CN107102000A (en) | 2017-08-29 |
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