CN106525724A - Centrifugal fully integrated detection system of methanal and pH value - Google Patents
Centrifugal fully integrated detection system of methanal and pH value Download PDFInfo
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
- B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0631—Purification arrangements, e.g. solid phase extraction [SPE]
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0887—Laminated structure
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/14—Means for pressure control
Abstract
The invention discloses a centrifugal fully integrated detection micro-fluidic chip of methanal and pH value. The micro-fluidic chip comprises a channel layer, the channel layer comprises a chip basic body and at least one sample detection unit, and the sample detection unit is arranged on the chip basic body. The sample detection unit comprises a liquid storage pool, a sample pool, a microfluid channel, a first detection pool, a second detection pool, a waste-liquid pool and a ventilation hole, wherein the liquid storage pool is communicated with the sample pool through the microfluid channel, the sample pool is communicated with the first detection pool and the second detection pool through the microfluid channel respectively, the second detection pool is communicated with the waste-liquid pool through the microfluid channel, and the waste-liquid pool is communicated with the ventilation hole through the microfluid channel. By adopting the centrifugal fully integrated detection micro-fluidic chip of methanal and pH value, extra detection agents do not need to be added during the detection process, professional personnel are not necessary, the operation is easy, and the methanal and pH value of multiple textile samples can be detected quickly and simultaneously with one sample adding.
Description
Technical field
The present invention relates to the detection field of formaldehyde and pH value, more particularly to a kind of centrifugal fully integrated formaldehyde and pH value
Detecting system.
Background technology
Conventional crosslinking agent of the formaldehyde as cellulose fibre resin finishing, being widely used in pure spinning or blending product (includes
Part true silk products), so as to give the functions such as shrinkproof, crease-resistant, the non-ironing and easy decontamination of textile.But, content of formaldehyde is exceeded
During dress or use, the free formaldehyde that part is uncrosslinked or hydrolysis is produced can be discharged textile, serious to damage
Health.At present, there are mandatory provisions in China to content of formaldehyde on textile, detects the method standard of formaldehyde content of textiles
It is GB/T2912.1 2009《The measure part 1 of textile formaldehyde：Formaldehyde (aqueous extraction method) that is free and hydrolyzing》, using purple
The analysis method of outward-visible absorption spectrophotometer (UV VIS).
The soda acid content that textile pH value is remained in referring to fabric, typically requires all in neutral range.PH value is detected also very
It is necessary, pH value is exceeded, its harm is no less than formaldehyde.The surface of human body skin is in faintly acid, so desirably prevents germ
Invade, if the acid-base value in clothes is exceeded, the faintly acid of human skin will be destroyed, so that the immunity of human body skin
Power is reduced.It is that the sensitive colony of partial skin, if wearing the exceeded clothes of pH value, has skin red that one intuitively shows
Swollen or itch etc. is uncomfortable.At present, also there are mandatory provisions in China to textile pH value, the method for detecting textile pH value
Standard is GB/T 7573-2009《The measure of textile aqueous extract liquor pH value》, pH value in textile is determined using PH meters.
Micro-fluidic chip (Microfluidics), be the sample preparation of biological, chemical laboratory analysis process, reaction,
The operating units such as separation, detection are integrated on one several square centimeters of chip, by the manipulation to fluid in microchannel network
And control, it is automatically performed analysis process.Its target is to realize the miniaturization of analytical equipment, integrated and automation, final to realize
The chip in laboratory, i.e., so-called " micro-total analysis system ".Compare with traditional chemistry, bioanalytical method, micro-fluidic core
Piece analysis and detection technology has miniaturization, integrated, high flux, the remarkable advantage of low cost, therefore, microfluidic chip technology exists
The fields such as clinical examination, drug screening, food security, environmental monitoring, judicial expertise, Chemical Manufacture, grind with great basis
Study carefully value, and wide industrialization prospect.Be developed so far, micro-fluidic chip become the current Environment Science field in the whole world it
One.
At present, the method to textile inspection of national regulations has very in terms of analysis limit, the degree of accuracy and reappearance
Big superiority, but detecting step is cumbersome, it is professional to have high demands, and formaldehyde and pH value can not be by the inspections simultaneously of once sample-adding
Survey, it is difficult to realize the purpose of field quick detection.Therefore, how to provide one kind to be once loaded, you can while the multiple weavings of detection
In product sample, the detecting system of formaldehyde and pH value two indices is the technical problem of those skilled in the art's urgent need to resolve.
The content of the invention
It is an object of the invention to provide the micro-fluidic chip of a kind of centrifugal fully integrated formaldehyde and pH value, once sample-adding is
Can simultaneously, formaldehyde and pH value two indices in the multiple testing samples of quick detection.
For achieving the above object, the invention provides following scheme：
A kind of centrifugal fully integrated formaldehyde and the micro-fluidic chip of pH value detection, the micro-fluidic chip include passage
Layer, the channel layer include chip basal body and at least one sample detection unit,
The sample detection unit is located in the chip basal body, and the sample detection unit includes：Liquid storage tank, sample
Pond, microfluidic channel, the first detection cell, the second detection cell, waste liquid pool and passage,
Wherein, the liquid storage tank is connected with the sample cell by the microfluidic channel, and the sample cell is by described
Microfluidic channel is connected with first detection cell and second detection cell respectively, and second detection cell passes through the miniflow
Body passage is connected with the waste liquid pool, and the waste liquid pool is connected with the passage by the microfluidic channel.
Optionally, the micro-fluidic chip also includes cover layer, and the cover layer is located above the channel layer, the lid
Flaggy is provided with opening, for throw in testing sample to the sample cell by described opening.
Optionally, the micro-fluidic chip also includes and the sample cell lid for being open and matching that the sample cell lid is located at
In the cover layer.
Optionally, the micro-fluidic chip also includes chip fixing hole, and the chip fixing hole is used for will be described micro-fluidic
Chip is fixed on the centrifugation apparatus.
Optionally, the liquid storage tank is internally provided with extraction liquid capsule, and the extract in the intracapsular portion of the extract is used for dissolution
Formaldehyde inside testing sample.
Optionally, the extraction liquid capsule is rupturable under external force, and the external force effect includes：Centrifugation extruding, machinery
Pressing and Manual press at least one.
Optionally, formaldehyde testing reagent is previously stored with first detection cell, is deposited in second detection cell in advance
Contain and in pH value detection reagent, or first detection cell, be previously stored with pH value detection reagent, in second detection cell
It is previously stored with formaldehyde testing reagent.
Optionally, the region material of cover layer correspondence first detection cell and second detection cell is transparent material
Material.
Optionally, the micro-fluidic includes：It is glass, dimethyl silicone polymer, polymethyl methacrylate, poly-
At least one in ethylene glycol terephthalate, polyethylene, polyvinyl chloride, polystyrene, polytetrafluoroethylene (PTFE) or polyester.
According to the specific embodiment that the present invention is provided, the invention discloses following technique effect：The centrifugation that the present invention is provided
The micro-fluidic chip of the fully integrated formaldehyde of formula and pH value, micro-fluidic chip liquid storage tank storage inside have extraction liquid capsule, inside liquid capsule
The formaldehyde that is used for inside dissolution testing sample of extract, be fixed with detection reagent in detection cell in advance, therefore add and treat test sample
It is after product, without the need for separately adding detection reagent, easy to operate, operating process is simplified, shortens detection time, can be complete without the need for professional
Into operation, the formaldehyde and pH value of multiple textile samples once by sample-adding, are detected simultaneously.
It is an object of the invention to provide the detecting system of a kind of centrifugal fully integrated formaldehyde and pH value, is once loaded
Simultaneously, formaldehyde and pH value two indices in the multiple textile samples of quick detection.
For achieving the above object, the invention provides following scheme：
The detecting system of a kind of centrifugal fully integrated formaldehyde and pH value, the detecting system include：
Described centrifugal fully integrated formaldehyde and the micro-fluidic chip of pH value detection；
Centrifugation apparatus, are fixedly installed the micro-fluidic chip on the centrifugation apparatus, for moving in the centrifugation apparatus
When drive the micro-fluidic chip to rotate, make liquid flowing in the micro-fluidic chip, and in first detection cell and described
Corresponding product is produced in second detection cell；And
Photoelectric detector, for detecting the anti-of first detection cell and second detection cell of the micro-fluidic chip
Product is answered, content of formaldehyde and pH value is determined respectively.
According to the specific embodiment that the present invention is provided, the invention discloses following technique effect：The centrifugation that the present invention is provided
The detecting system of the fully integrated formaldehyde of formula and pH value, it is after adding testing sample, without the need for separately adding detection reagent, easy to operate, once
Sample-adding can detect the formaldehyde and pH value of multiple testing samples simultaneously, be capable of achieving the purpose of field quick detection.
Description of the drawings
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below will be to institute in embodiment
The accompanying drawing that needs are used is briefly described, it should be apparent that, drawings in the following description are only some enforcements of the present invention
Example, for those of ordinary skill in the art, without having to pay creative labor, can be with according to these accompanying drawings
Obtain other accompanying drawings.
Fig. 1 is the channel layer structural representation for including a sample detection unit；
Fig. 2 is sample cell lid structure chart；
Fig. 3 is the cover layer structure chart matched with Fig. 1；
Fig. 4 is the channel layer structural representation for including three sample detection units；
Fig. 5 is the channel layer structural representation for including six sample detection units；
Fig. 6 is overhaul flow chart.
Symbol description：
1 sample detection unit of chip fixing hole, 2 chip basal body 3
Liquid storage tank 201 extracts 202 sample cell of liquid capsule, 203 microfluidic channel 204
First 205 second detection cell of detection cell, 206 waste liquid pool, 207 passage 208.
Specific embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than the embodiment of whole.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
It is an object of the invention to provide the microfluidic system of a kind of centrifugal fully integrated formaldehyde and pH value detection, micro-fluidic
Chip liquid storage tank storage inside has extraction liquid capsule, the formaldehyde that the extract inside liquid capsule is used for inside dissolution testing sample, detection
It is fixed with detection reagent in pond in advance, thus after adding testing sample, it is without the need for separately adding detection reagent, easy to operate, simplify behaviour
Make flow process, shorten detection time, operation can be completed without the need for professional, once sample-adding can detect multiple textile samples simultaneously
Formaldehyde and pH value, realize the purpose of field quick detection formaldehyde and pH value.
It is understandable to enable the above objects, features and advantages of the present invention to become apparent from, it is below in conjunction with the accompanying drawings and concrete real
The present invention is further detailed explanation to apply mode.
Embodiment 1：The centrifugal fully integrated formaldehyde and the micro-fluidic chip of pH value detection that the present invention is provided includes passage
Layer, cover layer, sample cell lid and chip fixing hole are illustrated in figure 1 the passage Rotating fields including a sample detection unit 2 and show
It is intended to, the sample cell lid is as shown in Figure 2.The chip fixing hole 1 is the through hole through the channel layer and the cover layer,
Its effect is to fix micro-fluidic chip, the micro-fluidic chip is fixed on centrifugation apparatus by the chip fixing hole 1, is protected
Card chip is firmly combined with not shifting in centrifugal process.The sample detection unit 2 is located in the chip basal body 3, institute
Stating sample detection unit 2 includes：Liquid storage tank 201, extraction liquid capsule 202, sample cell 203, microfluidic channel 204, the first detection cell
205th, the second detection cell 206, waste liquid pool 207 and passage 208, wherein, the liquid storage tank 201 is by the microfluidic channel
204 are connected with the sample cell 203, the sample cell 203 by the microfluidic channel 204 respectively with first detection cell
205 connect with second detection cell 206, and second detection cell 206 is by the microfluidic channel 204 and the waste liquid pool
207 connections, the waste liquid pool 207 are connected with the passage 208 by the microfluidic channel 204.
The structure chart of cover layer is illustrated in figure 3, the cover layer is located above the channel layer, with the channel layer
Match somebody with somebody.The cover layer is provided with opening, the open amount and 203 quantity Matching of sample cell, and just to each sample pond 203, uses
Testing sample is thrown in the sample cell 203 in described opening is passed through.The sample cell lid be located at the cover layer on, and with institute
Opening matching is stated, the sample cell lid can be the quantity of flip or swivelling cover etc., sample cell lid quantity and sample detection unit 2
Matching；The region material of corresponding first detection cell 205 of the cover layer and second detection cell 206 is transparent material,
It is penetrating to detection light to ensure.
The liquid storage tank is internally provided with extraction liquid capsule 202, and the extract extracted inside liquid capsule 202 is treated for dissolution
Survey the chemical composition of sample interior, such as formaldehyde.Extraction 202 face seal of liquid capsule, it can be various shapes to extract liquid capsule 202
Shape, the material for extracting liquid capsule 202 can be organic polymer film, aluminium foil, glass etc..Liquid capsule 202 is extracted with outer masterpiece
With the ability of lower rupture, external force effect can be centrifugation extruding, mechanical compression, Manual press etc..In first detection cell 205
Formaldehyde testing reagent is previously stored with, in second detection cell 206, pH value detection reagent is previously stored with.
The micro-fluidic includes：It is glass, dimethyl silicone polymer, polymethyl methacrylate, poly- to benzene two
At least one in formic acid glycol ester, polyethylene, polyvinyl chloride, polystyrene, polytetrafluoroethylene (PTFE) or polyester, chip material can
Being single, or the combination of above-mentioned material.
In addition, in above-described embodiment 1, the micro-fluidic chip also includes backplane level, below channel layer and with it is logical
Channel layer matches, and provides substrate for channel layer, with the effect for reinforcing sealing channel layer bottom.The chip fixing hole 1 is to pass through
The through hole of break-through channel layer, cover layer and backplane level.
In addition, in above-described embodiment 1, the channel layer can also include multiple sample detection units 2, can detect simultaneously
Multiple textile samples, the channel layer shown in Fig. 4 include three sample detection units 2, can detect three samples, Fig. 5 simultaneously
Shown channel layer includes 6 sample detection units 2, can detect 6 samples simultaneously.
The centrifugal fully integrated micro-fluidic chip that can detect textile formaldehyde and pH value simultaneously that the present invention is provided, chip storage
Liquid pool storage inside has extraction liquid capsule, the chemical composition that the extract inside liquid capsule is used for inside dissolution testing sample, detection cell
Detection reagent is inside fixed with advance, therefore after adding testing sample, detection reagent need not be configured during operation, simplify operation stream
Journey, without the need for professional, is used in conjunction with supporting portable set, achievable scene simultaneously, in the multiple samples of quick detection formaldehyde and
The purpose of pH value.
Embodiment 2：The present invention also provides the detecting system of a kind of centrifugal fully integrated formaldehyde and pH value, the detection system
System includes：Above-mentioned centrifugal fully integrated formaldehyde and the micro-fluidic chip of pH value detection；
Centrifugation apparatus, are fixedly installed the micro-fluidic chip on the centrifugation apparatus, for moving in the centrifugation apparatus
When drive the micro-fluidic chip to rotate, make liquid flowing in the micro-fluidic chip, and in first detection cell and described
Corresponding product is produced in second detection cell；And
Photoelectric detector, for detecting the anti-of first detection cell and second detection cell of the micro-fluidic chip
Product is answered, content of formaldehyde and pH value is determined respectively.
The chip manipulates fluid operation by being centrifuged, and can detect formaldehyde and pH value simultaneously.Liquid when being illustrated in figure 6 detection
The flow process of body flowing, during use, opens sample cell lid, and the textile to be measured of the suitable size of clip is put into sample cell, covers sample
Then chip is put into supporting detector by Chi Gai, broken to extract the extract that liquid capsule release chip internal is prestored, and is centrifuged,
Extract is contacted into sample cell with textile to be measured, the formaldehyde in the tested textile of dissolution, is centrifuged, makes prepare liquid after several minutes
Body respectively enters formaldehyde and pH value detection cell, is reacted with the reagent being fixed in detection cell, and product passes through light electric-examination
Instrument detection is surveyed, it is final to realize simplicity, quickly detect.Wherein, formaldehyde and pH value inspection are prestored in formaldehyde and pH value detection cell
Test agent, storage mode are included directly in chip base or (commercialization filter paper, perforated membrane, designed, designed coagulate in solid phase carrier
Glued membrane etc.) on immobilization.
Centrifugal fully integrated formaldehyde and the detecting system of pH value that the present invention is provided, after adding testing sample, without the need for another
Outer reagent adding again, it is easy to operate, operating process is simplified, detection time is shortened, without the need for professional, you can realize that scene is fast
The purpose of formaldehyde and pH value in the multiple samples of speed detection.
In this specification, each embodiment is described by the way of progressive, and what each embodiment was stressed is and other
The difference of embodiment, between each embodiment identical similar portion mutually referring to.
Specific case used herein is set forth to the principle and embodiment of the present invention, and above example is said
It is bright to be only intended to help and understand the method for the present invention and its core concept；Simultaneously for one of ordinary skill in the art, foundation
The thought of the present invention, will change in specific embodiments and applications.In sum, this specification content is not
It is interpreted as limitation of the present invention.
Claims (10)
1. a kind of micro-fluidic chip that centrifugal fully integrated formaldehyde and pH value are detected, it is characterised in that the micro-fluidic chip
Including channel layer, the channel layer includes chip basal body and at least one sample detection unit,
The sample detection unit is located in the chip basal body, and the sample detection unit includes：It is liquid storage tank, sample cell, micro-
Fluid passage, the first detection cell, the second detection cell, waste liquid pool and passage,
Wherein, the liquid storage tank is connected with the sample cell by the microfluidic channel, and the sample cell passes through the miniflow
Body passage is connected with first detection cell and second detection cell respectively, and second detection cell is logical by the microfluid
Road is connected with the waste liquid pool, and the waste liquid pool is connected with the passage by the microfluidic channel.
2. the micro-fluidic chip that centrifugal fully integrated formaldehyde according to claim 1 and pH value are detected, it is characterised in that
The micro-fluidic chip also includes cover layer, and the cover layer is located above the channel layer, and the cover layer is provided with opening,
For throw in testing sample to the sample cell by described opening.
3. the micro-fluidic chip that centrifugal fully integrated formaldehyde according to claim 2 and pH value are detected, it is characterised in that
The micro-fluidic chip also includes and the sample cell lid for being open and matching that the sample cell lid is located in the cover layer.
4. the micro-fluidic chip that centrifugal fully integrated formaldehyde according to claim 1 and pH value are detected, it is characterised in that
The micro-fluidic chip also includes chip fixing hole, the chip fixing hole for by the micro-fluidic chip be fixed on it is described from
On heart equipment.
5. the micro-fluidic chip that centrifugal fully integrated formaldehyde according to claim 1 and pH value are detected, it is characterised in that
The liquid storage tank is internally provided with extraction liquid capsule, the first that the extract in the intracapsular portion of the extract is used for inside dissolution testing sample
Aldehyde.
6. the micro-fluidic chip that centrifugal fully integrated formaldehyde according to claim 5 and pH value are detected, it is characterised in that
The extraction liquid capsule is rupturable under external force, and the external force effect includes：Centrifugation extruding, in mechanical compression and Manual press
At least one.
7. the micro-fluidic chip that centrifugal fully integrated formaldehyde according to claim 1 and pH value are detected, it is characterised in that
Formaldehyde testing reagent is previously stored with first detection cell, pH value detection examination in second detection cell, is previously stored with
PH value detection reagent is previously stored with agent, or first detection cell, in second detection cell, formaldehyde is previously stored with
Detection reagent.
8. the micro-fluidic chip that centrifugal fully integrated formaldehyde according to claim 1 and pH value are detected, it is characterised in that
The region material of cover layer correspondence first detection cell and second detection cell is transparent material.
9. centrifugal fully integrated formaldehyde according to any one of claim 1-8 and the micro-fluidic chip of pH value detection,
Characterized in that, the micro-fluidic includes：It is glass, dimethyl silicone polymer, polymethyl methacrylate, poly- to benzene
At least one in naphthalate, polyethylene, polyvinyl chloride, polystyrene, polytetrafluoroethylene (PTFE) or polyester.
10. the detecting system of a kind of centrifugal fully integrated formaldehyde and pH value, it is characterised in that the detecting system includes：
The micro-fluidic chip of the centrifugal fully integrated formaldehyde and pH value detection any one of claim 1-9；
Centrifugation apparatus, are fixedly installed the micro-fluidic chip on the centrifugation apparatus, for the band when the centrifugation apparatus are moved
The dynamic micro-fluidic chip is rotated, and makes liquid flowing in the micro-fluidic chip, and in first detection cell and described second
Corresponding product is produced in detection cell；And
Photoelectric detector, for detecting that the reaction of first detection cell and second detection cell of the micro-fluidic chip is produced
Thing, determines content of formaldehyde and pH value respectively.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2016109437520 | 2016-10-26 | ||
| CN201610943752 | 2016-10-26 |
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| CN106525724A true CN106525724A (en) | 2017-03-22 |
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| CN201610979904.2A Pending CN106525724A (en) | 2016-10-26 | 2016-11-08 | Centrifugal fully integrated detection system of methanal and pH value |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108732339A (en) * | 2017-04-19 | 2018-11-02 | 光宝电子(广州)有限公司 | Runner design and its detection method for multiple reaction biological detection |
| CN109536382A (en) * | 2019-01-08 | 2019-03-29 | 杭州霆科生物科技有限公司 | A kind of water quality comprehensive toxicity detection chip |
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| CN109536381A (en) * | 2019-01-08 | 2019-03-29 | 杭州霆科生物科技有限公司 | A kind of centrifugal water quality comprehensive toxicity detection chip |
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| CN111545261A (en) * | 2020-05-14 | 2020-08-18 | 杭州霆科生物科技有限公司 | Textile formaldehyde and pH value detection device and method |
| CN111545261B (en) * | 2020-05-14 | 2021-10-15 | 杭州霆科生物科技有限公司 | Textile formaldehyde and pH value detection device and method |
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