CN110196249A - A kind of paper chip of integration standard colour band - Google Patents
A kind of paper chip of integration standard colour band Download PDFInfo
- Publication number
- CN110196249A CN110196249A CN201810164046.5A CN201810164046A CN110196249A CN 110196249 A CN110196249 A CN 110196249A CN 201810164046 A CN201810164046 A CN 201810164046A CN 110196249 A CN110196249 A CN 110196249A
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- Prior art keywords
- colour
- colour band
- color
- paper chip
- developing area
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/7756—Sensor type
- G01N2021/7759—Dipstick; Test strip
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- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The present invention provides a kind of paper chip of integration standard colour band, including reference colour zone and detection zone.Reference colour zone is made of colour band and label character, the color lump that each colour band has several linearly aligned color intensities to change in gradient, and marks concentration beside corresponding color lump.Detection zone includes colour developing area.Paper chip provided by the invention, from integration standard colour band, detection carries out colour developing area every time and reference colour band compares, it is used in conjunction with mating portable device, individual difference and ambient light when reducing colorimetric detection influence, it is able to achieve and quick and precisely detects, but also being capable of visual colorimetric determination observation experiment result.
Description
Technical field
The present invention relates to paper chip technical fields, more particularly to a kind of paper chip of integration standard colour band.
Background technique
Paper chip (Paper-based Microfluidics) is the brand new technical just occurred in 2007, the technology
Chip manufacturing material and biochemical analysis are used as using paper (sensu lato paper material such as filter paper, chromatographic paper and nitrocellulose filter)
Platform.Paper chip has that production method is easy, low in cost, small in size, light-weight, convenient for storage and transport, biocompatibility
Good, sample/reagent consumption is less, analysis speed is fast, subsequent processing is simple, itself porous structure can realize sample transport, without outer
The advantages such as boundary's driving force.There is good application prospect in fields such as medical diagnosis, environmental monitoring, food hygienes.
Colorimetric detection method is one of the most common type detection method in current paper chip analysis and research.Usual chromogenic reaction, makes
Component to be detected is converted into coloured substance, using the correlation of color depth and concentration of component to be measured, carries out quantitative or half
Quantitative analysis.This detection can directly be distinguished by naked eyes using ocular estimate, carried out semi-quantitative analysis, can also be used number
Then code camera or taking photograph of intelligent mobile phone scanner scanning are handled at image using image processing software.By color of image
Gray value is converted to, quantitative analysis is carried out according to the relationship between gray value and testing concentration.Ocular estimate by observer's subjectivity because
Element is affected, and detects after scanning of taking pictures and has no reference colour band at present while carrying out scanning comparison of taking pictures, there are chip individual differences
It is different to influence error with ambient light.
Summary of the invention
In view of the above-mentioned problems of the prior art, the object of the present invention is to provide a kind of cores of integration standard colour band
Piece.
To achieve the above object, the present invention provides following schemes:
The present invention provides a kind of paper chips of integration standard colour band, which is characterized in that the paper chip includes substrate, the base
Reference colour zone and detection zone are set on material；
The reference colour zone is made of colour band and label character, and each colour band has several linearly aligned color intensities in gradient
The color lump of variation, and concentration is marked beside corresponding color lump.
Optionally, the color lump color of the colour band and homochromy, the color lump quantity root after corresponding index chromogenic reaction to be measured
It is adjusted according to index concentration range to be measured with precision, is 3~20.
Optionally, the pattern that each color lump is formed on the substrate is band-like or dotted, and each color lump
Pattern width or diameter be 0.1~10 millimeter.
Optionally, the detection zone includes colour developing area, and the colour developing area, which is fixed with, chromogenic reaction occurs with determinand
Color developing agent.
Optionally, the detection zone further includes adding mouth and microchannel, and the adding mouth and the colour developing area are by miniflow
Channel connection.
Optionally, the adding mouth, microchannel and colour developing area are hydrophilic area, are around water delivery area.
Optionally, detection when colour developing area be added prepare liquid, alternatively, detection when adding mouth be added prepare liquid, it is described to
It surveys liquid and flows into colour developing area through microchannel；Chromogenic reaction occurs for fixed color developing agent in the prepare liquid and colour developing area, after reaction
The reference colour band comparison carried in the color and chip of display, by necessary instrument or visually determines prepare liquid concentration.
The specific embodiment provided according to the present invention, the invention has the benefit that
Paper chip provided by the invention, from integration standard colour band, detection carries out colour developing area every time and reference colour band compares, and matches
Set portable device is used in conjunction, and the chip individual difference and ambient light when reducing colorimetric detection influence, and is able to achieve quick and precisely
Detection, but also being capable of visual colorimetric determination observation experiment result.
Detailed description of the invention
It in order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, below will be to institute in embodiment
Attached drawing to be used is needed to be briefly described, it should be apparent that, the accompanying drawings in the following description is only some implementations of the invention
Example, for those of ordinary skill in the art, without any creative labor, can also be according to these attached drawings
Obtain other attached drawings.
Fig. 1 is that the single index of one of embodiment of the present invention integration standard colour band detects paper chip schematic diagram；
Fig. 2 is that the Two indices of one of embodiment of the present invention integration standard colour band detect paper chip schematic diagram；
Fig. 3 is that the single index of one of embodiment of the present invention integration standard colour band detects micro-fluidic paper chip schematic diagram；
Fig. 4 is the micro-fluidic paper chip schematic diagram of three Indexs measures of one of embodiment of the present invention integration standard colour band.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
A kind of embodiment 1: single index detection paper chip of integration standard colour band
As shown in Figure 1, a kind of single index of integration standard colour band detects paper chip, including substrate 1, standard is set on the substrate
Colour band area 2 and detection zone 3.The reference colour zone 2 is made of colour band 21 and label character 23, and colour band 21 has several linear arrays
The color lump 22 that changes in gradient of color intensity, and mark concentration beside corresponding color lump 22.22 face of color lump of the colour band 21
Color is homochromy with after corresponding index chromogenic reaction to be measured, and 22 quantity of color lump is adjusted according to index concentration range to be measured with precision
It is whole, it is 3~20.The pattern that each color lump 22 is formed on the substrate 1 is band-like or dotted, and the pattern of each color lump 22
Width or diameter are 0.1~10 millimeter.The detection zone 3 includes colour developing area 31, and the colour developing area 31 is fixed with energy and determinand
The color developing agent of chromogenic reaction occurs.The colour developing area 31 is hydrophilic area, is around water delivery area.
Prepare liquid is added in colour developing area 31 when detection, the prepare liquid develops the color with color developing agent fixed in colour developing area 31
It reacts, the reference colour zone 2 carried in the color and chip shown after reaction compares, and by necessary instrument or visually determines to be measured
Liquid concentration.
A kind of embodiment 2: Two indices detection paper chip of integration standard colour band
As shown in Fig. 2, a kind of Two indices of integration standard colour band detect paper chip, including substrate 1, standard is set on the substrate
Colour band area 2 and detection zone 3.The reference colour zone 2 is made of two colour bands 21 and label character 23, and each colour band 21 has several
The color lump 22 that linearly aligned color intensity changes in gradient, and concentration is marked beside corresponding color lump 22, by each colour band 21
Side marks index name.22 color of color lump of the colour band 21 and homochromy, the color lump 22 after corresponding index chromogenic reaction to be measured
Quantity is adjusted according to index concentration range to be measured with precision, is 3~20.What each color lump 22 was formed on the substrate 1
Pattern is band-like or dotted, and the pattern width of each color lump 22 or diameter are 0.1~10 millimeter.The detection zone 3 includes
Two colour developing areas 31, and corresponding Testing index title is marked beside each colour developing area 31.The colour developing area 31 is fixed with can be with
The color developing agent of determinand generation chromogenic reaction.The colour developing area 31 is hydrophilic area, is around water delivery area.
Prepare liquid is added in colour developing area 31 when detection, the prepare liquid develops the color with color developing agent fixed in colour developing area 31
Reaction, the color shown after reaction are compared with the corresponding reference colour zone 2 carried on chip, by necessary instrument or are visually determined
Prepare liquid concentration.
A kind of embodiment 3: micro-fluidic paper chip of single index detection of integration standard colour band
As shown in figure 3, a kind of single index of integration standard colour band detects micro-fluidic paper chip, including substrate 1, set on the substrate
Set reference colour zone 2 and detection zone 3.The reference colour zone 2 is made of colour band 21 and label character 23, if colour band 21 has main line
Property arrangement the color lump 22 that changes in gradient of color intensity, and mark concentration beside corresponding color lump 22.The color of the colour band 21
22 color of block is homochromy with after corresponding index chromogenic reaction to be measured, and 22 quantity of color lump is according to index concentration range to be measured and precision
It is adjusted, is 3~20.The pattern that each color lump 22 is formed on the substrate is band-like or dotted, and each color lump 22
Pattern width or diameter be 0.1~10 millimeter.The detection zone 3 includes colour developing area 31, and the colour developing area 31 is fixed with can be with
The color developing agent of determinand generation chromogenic reaction.The detection zone 3 further includes adding mouth 33 and microchannel 32, the adding mouth 33
It is connected to the colour developing area 31 by microchannel 32.The adding mouth 33, microchannel 32 and colour developing area 31 are hydrophilic area, surrounding
For water delivery area.
Prepare liquid is added in adding mouth 33 when detection, the prepare liquid flows into colour developing area 31 through microchannel 32, passes through paper
Chip channel removes partial impurities；Chromogenic reaction occurs for fixed color developing agent in the prepare liquid and colour developing area 31, shows after reaction
The reference colour zone 2 carried in the color and chip shown compares, and by necessary instrument or visually determines prepare liquid concentration.
A kind of embodiment 4: micro-fluidic paper chip of three Indexs measures of integration standard colour band
As shown in figure 4, a kind of micro-fluidic paper chip of three Indexs measures of integration standard colour band, including substrate 1, set on the substrate
Set reference colour zone 2 and detection zone 3.The reference colour zone 2 is made of three colour bands 21 and label character 23, if colour band 21 has
The color lump 22 that linearly aligned color intensity changes in gradient is done, and marks concentration, each colour band 21 beside corresponding color lump 22
Side marks index name.22 color of color lump of the colour band 21 and homochromy, the color lump after corresponding index chromogenic reaction to be measured
22 quantity are adjusted according to index concentration range to be measured with precision, are 3~20.What each color lump 22 was formed on the substrate
Pattern is band-like or dotted, and the pattern width of each color lump 22 or diameter are 0.1~10 millimeter.The detection zone 3 includes
Three colour developing areas 31, and corresponding Testing index title is marked beside each colour developing area 31.Each colour developing area 31 is fixed with can be with
The color developing agent of chromogenic reaction occurs for determinand, can be carried out three different index inspections of same sample according to fixed color developing agent type
It surveys, also can be carried out the same index Parallel testing of same sample.The detection zone 3 further includes adding mouth 33 and microchannel 32,
The adding mouth 33 is connected to the colour developing area 31 by microchannel 32.The adding mouth 33, microchannel 32 and colour developing area 31
It is around water delivery area for hydrophilic area.
Prepare liquid is added in adding mouth 33 when detection, the prepare liquid flows into colour developing area 31 through microchannel 32, passes through paper
Chip channel removes partial impurities；Chromogenic reaction occurs for fixed color developing agent in the prepare liquid and colour developing area 31, shows after reaction
The color shown is compared with the corresponding reference colour zone 2 carried on chip, by necessary instrument or visually determines prepare liquid concentration.
Used herein a specific example illustrates the principle and implementation of the invention, and above embodiments are said
It is bright to be merely used to help understand method and its core concept of the invention；At the same time, for those skilled in the art, foundation
Thought of the invention, there will be changes in the specific implementation manner and application range.In conclusion the content of the present specification is not
It is interpreted as limitation of the present invention.
Claims (7)
1. a kind of paper chip of integration standard colour band, which is characterized in that the paper chip includes substrate, and mark is arranged on the substrate
Quasi- colour band area and detection zone；
The reference colour zone is made of colour band and label character, and each colour band has several linearly aligned color intensities in gradient
The color lump of variation, and concentration is marked beside corresponding color lump.
2. a kind of paper chip of integration standard colour band according to claim 1, which is characterized in that the color lump color of the colour band
Homochromy with after corresponding index chromogenic reaction to be measured, the color lump quantity is adjusted according to each index concentration range to be measured with precision
It is whole, it is 3~20.
3. a kind of paper chip of integration standard colour band according to claim 1, which is characterized in that each color lump is in the base
The pattern formed on material is band-like or dotted, and the pattern width of each color lump or diameter are 0.1~10 millimeter.
4. a kind of paper chip of integration standard colour band according to claim 1, which is characterized in that the detection zone includes colour developing
Area, the colour developing area are fixed with the color developing agent that chromogenic reaction can occur with determinand.
5. a kind of paper chip of integration standard colour band according to claim 1, which is characterized in that the detection zone further includes adding
Sample mouth and microchannel, the adding mouth and the colour developing area are connected to by the microchannel.
6. a kind of paper chip of integration standard colour band according to claim 1, which is characterized in that the adding mouth, miniflow are logical
Road and colour developing area are hydrophilic area, are around water delivery area.
7. a kind of paper chip of integration standard colour band according to claim 1, which is characterized in that be added when detection in colour developing area
Prepare liquid, alternatively, prepare liquid is added in adding mouth when detection, the prepare liquid flows into colour developing area through microchannel；It is described to be measured
Chromogenic reaction, the reference colour band pair carried in the color shown after reaction and chip occur for fixed color developing agent in liquid and colour developing area
Than by necessary instrument or visually determining prepare liquid concentration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810164046.5A CN110196249A (en) | 2018-02-27 | 2018-02-27 | A kind of paper chip of integration standard colour band |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810164046.5A CN110196249A (en) | 2018-02-27 | 2018-02-27 | A kind of paper chip of integration standard colour band |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110196249A true CN110196249A (en) | 2019-09-03 |
Family
ID=67751059
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810164046.5A Pending CN110196249A (en) | 2018-02-27 | 2018-02-27 | A kind of paper chip of integration standard colour band |
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| Country | Link |
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| CN (1) | CN110196249A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114317678A (en) * | 2021-12-31 | 2022-04-12 | 港龙生物技术（深圳）有限公司 | Biological paper chip, high-flux multi-connection detection microporous plate device, preparation method and kit for multi-connection detection of vaginitis |
| US11498069B2 (en) | 2020-08-31 | 2022-11-15 | International Business Machines Corporation | Rapid testing device for wastewater analysis |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201852790U (en) * | 2010-11-24 | 2011-06-01 | 胡平安 | Semi-quantitative colorimetric blood sugar test paper |
| CN105921187A (en) * | 2016-05-23 | 2016-09-07 | 浙江清华长三角研究院 | Micro-fluidic chip for rapid detection of pesticide residues |
| CN106000487A (en) * | 2016-05-16 | 2016-10-12 | 南京工业大学 | Preparingmethod for hydrophobic and oleophobicmicrofluidics paper chip based on fluorine material |
-
2018
- 2018-02-27 CN CN201810164046.5A patent/CN110196249A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201852790U (en) * | 2010-11-24 | 2011-06-01 | 胡平安 | Semi-quantitative colorimetric blood sugar test paper |
| CN106000487A (en) * | 2016-05-16 | 2016-10-12 | 南京工业大学 | Preparingmethod for hydrophobic and oleophobicmicrofluidics paper chip based on fluorine material |
| CN105921187A (en) * | 2016-05-23 | 2016-09-07 | 浙江清华长三角研究院 | Micro-fluidic chip for rapid detection of pesticide residues |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11498069B2 (en) | 2020-08-31 | 2022-11-15 | International Business Machines Corporation | Rapid testing device for wastewater analysis |
| CN114317678A (en) * | 2021-12-31 | 2022-04-12 | 港龙生物技术（深圳）有限公司 | Biological paper chip, high-flux multi-connection detection microporous plate device, preparation method and kit for multi-connection detection of vaginitis |
| CN114317678B (en) * | 2021-12-31 | 2023-11-10 | 港龙生物技术（深圳）有限公司 | Biological paper chip, high-throughput multi-detection microplate device, preparation method and kit for vaginal inflammation multi-detection |
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Application publication date: 20190903 |
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