CN110470856A - The manual injector and sample injection method of controllable sampling volume and its application - Google Patents
The manual injector and sample injection method of controllable sampling volume and its application Download PDFInfo
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- CN110470856A CN110470856A CN201810448106.6A CN201810448106A CN110470856A CN 110470856 A CN110470856 A CN 110470856A CN 201810448106 A CN201810448106 A CN 201810448106A CN 110470856 A CN110470856 A CN 110470856A
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- 238000005070 sampling Methods 0.000 title claims abstract description 33
- 238000002347 injection Methods 0.000 title claims abstract description 25
- 239000007924 injection Substances 0.000 title claims abstract description 25
- 238000012545 processing Methods 0.000 claims description 11
- 238000007789 sealing Methods 0.000 claims description 4
- 238000001514 detection method Methods 0.000 claims description 3
- 238000003745 diagnosis Methods 0.000 claims description 3
- 238000003912 environmental pollution Methods 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 238000000338 in vitro Methods 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- 239000012491 analyte Substances 0.000 claims description 2
- 230000006835 compression Effects 0.000 abstract description 2
- 238000007906 compression Methods 0.000 abstract description 2
- 238000006243 chemical reaction Methods 0.000 description 18
- 230000000694 effects Effects 0.000 description 7
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- 239000000047 product Substances 0.000 description 3
- 230000006641 stabilisation Effects 0.000 description 3
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- 229920002319 Poly(methyl acrylate) Polymers 0.000 description 2
- 239000000463 material Substances 0.000 description 2
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- 229920005989 resin Polymers 0.000 description 2
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- 229910002027 silica gel Inorganic materials 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
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- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000003729 cation exchange resin Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
- G01N35/1016—Control of the volume dispensed or introduced
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0689—Sealing
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
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- General Health & Medical Sciences (AREA)
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Abstract
The present invention relates to sample injector field, manual injector and sample injection method and its application of a kind of controllable sampling volume are disclosed.The manual injector includes sample cell (2), sample cell lid (1) and sample portion (3) out；The sample cell lid (1) seals the sample cell (2) and forms sample cavity, described sample portion (3) out are located at the side opposite with the sample cell lid (1) of the sample cell (2), it can change the volume of the sample cavity by the movement of the sample cell lid (1), and the sample in the sample cavity extruded by going out sample portion (3).Manual injector of the invention increases the method sample introduction of pressure by manual compression air, pressure change is mildly controllable, the unstable phenomenon of sample introduction caused by fluctuation of pressure is avoided, and avoids the use of external sample introduction equipment, greatly reduces the volume and system complexity of sampling device.
Description
Technical field
The present invention relates to sample injector fields, and in particular to the manual injector and sample injection method of a kind of controllable sampling volume and
It is applied.
Background technique
While world economy and industrial expansion are that the mankind bring huge convenient, but also pollutant is emitted on quantity
It is continuously increased in type, environmental issue is on the rise.Greatly developing all kinds of high-precision analysis instruments and detection technique
Meanwhile the analytical technology of small portable, simple and fast also becomes a big research hotspot, it is numerous because operating to supplement large-scale instrument
It is trivial, maintenance workload is larger, it is high to require laboratory environment, it is difficult to the deficiency for on-site test.
Micro-total analysis system is also known as chip lab, be intended to by laboratory functional one be integrated on a chip
Kind analytical technology, brilliant prospect is shown in terms of the miniaturization of analytical technology, portability, automation, is become in recent years
One big research hotspot.
The process that sample introduces micro-total analysis system has large effect to entire analytic process and result, and micro- at present complete point
The sample introduction of analysis system is carried out typically by means of pressure difference, such as reduces stream using Micropump increase injection end pressure or using centrifugal pump
Enter end pressure, introduces process and often generate fluctuation because pressure difference is larger, and these equipment volumes are much larger than micro-total analysis chip
Volume, thus considerably increase the volume and complexity of micro-total analysis system.
Summary of the invention
The purpose of the invention is to overcome the problems such as sample injector device of the prior art is complicated, volume is big, provide controllable
The manual injector and sample injection method of sampling volume and its application, the manual injector increase pressure by manual compression air
Strong method sample introduction, pressure change is mildly controllable, avoids the unstable phenomenon of sample introduction caused by fluctuation of pressure, and avoid outside
The use of sample introduction equipment greatly reduces the volume and system complexity of sampling device.
To achieve the goals above, one aspect of the present invention provides a kind of manual injector of controllable sampling volume, this is manually
Sample injector includes sample cell, sample cell lid and sample portion out；The sample cell lid seals the sample cell and forms sample cavity, it is described go out
Sample portion is located at the side opposite with the sample cell lid of the sample cell, can change institute by the movement of the sample cell lid
The volume of sample cavity is stated, and the sample in the sample cavity is extruded by going out sample portion.
Preferably, scale is provided on the side wall of the sample cell, the scale is for indicating sample cell lid and the sample
The relative position in product pond.
Preferably, the sample cell lid and the sample cell are detachably connected.
Preferably, the sample cell and the sample cell lid are cylindricality.
Preferably, the sample cell lid is screwed on the sample cell.
Preferably, the middle part of the side opposite with the sample cell lid of the sample cell is arranged in the sample portion out.
Preferably, the sample portion out has helicitic texture or flexible seal member；It is highly preferred that the helicitic texture is interior
Helicitic texture or external thread structure；It is highly preferred that the flexible seal member is rubber stopper.
Preferably, the sample portion out includes sample output passage.
Preferably, the sample cell is transparent or semitransparent sample cell, and the sample cell lid is transparent or semitransparent sample
Chi Gai.
Preferably, the bottom of the sample cell is provided with sample processing element.
It is highly preferred that the sample processing element is in sample filtering component, example enrichment component and sample separation unit
It is one or more.
Second aspect of the present invention provides a kind of micro-fluidic chip, and the micro-fluidic chip has injection port, the injection port
Helicitic texture is set, and the helicitic texture of the helicitic texture and above-mentioned manual injector matches.
Third aspect present invention provides a kind of hand sampling method of controllable sampling volume, mobile above-mentioned manual injector
Sample cell lid extrudes the sample in the sample cavity by going out sample portion.
Preferably, the sample cell lid is screwed on the sample cell, and the mobile sample cell lid is by rotating the sample
Chi Gai is carried out.
Preferably, this method comprises: being determined by the scale being arranged on the side wall of the sample cell through the sample out
The volume for the sample that portion extrudes.
Preferably, this method comprises: the manual injector and micro-fluidic chip as claimed in claim 9 are screwed on, make
Sample in the manual injector enters the micro-fluidic chip.
Fourth aspect present invention also provides above-mentioned manual injector, above-mentioned micro-fluidic chip or above-mentioned hand sampling method and exists
The application in the fields such as environmental pollution analyte detection, in-vitro diagnosis, micro-total analysis chip.
Through the above technical solutions, compared with prior art, the manual injector of controllable sampling volume provided by the invention
It has the following technical effect that and 1) manually completes sample introduction process, avoid greatly reducing micro-total analysis using external equipments such as Micropumps
The volume and complexity of system；2) by can be manually rotated the position change sample introduction pressure of helicitic texture, pressure change is made mildly may be used
Control；3) rotatable helicitic texture can effectively fix position, prevent pressure increase in chip from making that nut is caused to rise, cause to press
Fluctuation influences sample introduction effect；4) manual injector bottom increases the convenience and two of handling with helicitic texture access chip
The air-tightness of person junction effectively prevent the generation that sample overflows when sample introduction.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the manual injector loading state of controllable sampling volume of the invention；
Fig. 2 is the structural schematic diagram of the manual injector use state of controllable sampling volume of the invention；
Fig. 3 is the structural schematic diagram of micro-fluidic chip of the invention.
Description of symbols
1, sample cell lid 2, sample cell 3, out sample portion
4, external screw thread 5, injection port 6, the first reaction tank
7, the second reaction tank 8, sample output passage
Specific embodiment
The endpoint of disclosed range and any value are not limited to the accurate range or value herein, these ranges or
Value should be understood as comprising the value close to these ranges or value.For numberical range, between the endpoint value of each range, respectively
It can be combined with each other between the endpoint value of a range and individual point value, and individually between point value and obtain one or more
New numberical range, these numberical ranges should be considered as specific open herein.
In the present invention, in the absence of explanation to the contrary, the noun of locality used such as " upper and lower, left and right " typically refers to
Upper and lower, left and right in attached drawing.
The manual injector of controllable sampling volume according to the present invention, as shown in Figs. 1-2, which includes sample
Pond 2, sample cell lid 1 and sample portion 3 out；The sample cell lid 1 seals the sample cell 2 and forms sample cavity, and the sample portion 3 out is located at
The side opposite with the sample cell lid 1 of the sample cell 2 can change the sample by the movement of the sample cell lid 1
The volume of product chamber, and the sample in the sample cavity is extruded by going out sample portion 3.
In the present invention, sample cell lid 1 is opened as shown in Figure 1, sample is placed in sample cell, and mobile example pond lid 1,
As shown in Fig. 2, sample is under the effect of the pressure, it can be forced out from manual injector, so as to complete sample introduction process.Pass through
The movement sample cell lid appropriate can achieve the purpose that control sampling volume.The sample portion 3 out can be set to from institute
The protrusion of sample cell 2 is stated, also can be set inside the sample cell, such as 3 bottom of sample portion is flushed with sample bottom of pond portion out.
Specific sampling volume is intuitively determined for convenience, it is preferable that is provided with quarter on the side wall of the sample cell 2
Degree, the scale are used to indicate the relative position of sample cell lid 1 Yu the sample cell 2.That is, the scale indicates the sample
The volume of chamber.Sample cell lid 1 and 2 relative position of sample cell are shown by setting scale, it can be intuitive during sample-adding
Ground judges sample-adding amount, may thereby determine that sampling volume.For more accurate sample introduction, the sample cell 2 and the sample cell lid 1
The sampling volume that size can according to need is selected, so that the scale more accurately shows the sample cell lid 1
Position, that is, selection small cross sections are long-pending when needing less sample volume and the sample cell 2 of more big height.
In the present invention, the amount that the scale can according to need the sample of addition is configured, in order to guarantee scale standard
Really show the volume of addition sample, scale can unevenly be set according to the volume of sample and air inside sample cavity
It sets.
According to the present invention, for the ease of being added sample into the manual injector, the sample cell lid 1 and the sample
Pond 2 is detachably connected.When needing that sample is added, sample cell lid 1 is opened, as shown in Figure 1, closing institute again after sample is added
State sample cell 2.
A preferred embodiment according to the present invention, for the ease of the movement sample cell lid 1, the sample cell 2
It is cylindricality with the sample cell lid 1, is more preferably cylinder.Specifically, the sample cell 2 and the sample cell lid 1 can
To be formed as tube-in-tube structure, wherein the sample cell lid 1 can be used as outer cylinder or inner cylinder.From the point of view of convenient for sample-adding,
It is preferred that the sample cell lid 1 is used as outer cylinder, the sample cell 2 is used as inner cylinder.
In the present invention, there is no particular limitation for the connection type of the sample cell lid 1 and the sample cell 2, such as can
So that the sample cell 2 and the sample cell lid 1 are formed as tube-in-tube structure, and in inner cylindrical at external screw thread, outer cylinder be formed with
Matched internal screw thread, by above-mentioned helicitic texture the sample cell lid 1 is screwed on the sample cell 2.At this point it is possible to logical
It crosses and rotates the sample cell lid 1 volume of sample cavity is changed.It is screwed on by making to state sample cell lid 1 with the sample cell 2, it can
So that the process of rotating sample pool lid 1 is more easier to control, to be easier accurate control loading process.It is revolved in sample cell lid 1
During, sample cell lid 1 can be fixed at an arbitrary position, and even if loosing one's grip, the position of sample cell lid 1 also can be by screw thread
It blocks, without guaranteeing the stabilization of pressure in sample cell there is a phenomenon where retracting, so that the liquid in micro-total analysis chip is not yet
It can flow back.
In the present invention, soft seal component can also be set (such as on the sample cell lid 1 or the sample cell 2
Rubber seal etc.) achieve the purpose that sealing forms sample cavity, at this point it is possible to make sample by sample cell lid 1 described in pull
The volume of chamber changes, and completes sample introduction process.
According to the present invention, there is no particular limitation for the position that sample portion 3 is arranged described out, is preferably provided at the sample cell 2
The side opposite with the sample cell lid 1 middle part.
According to the present invention, device to be loaded (such as micro-fluidic core is fully entered to guarantee sample during sample-adding
Piece) in, it is preferably tightly connected the sample-adding portion and device to be loaded, specifically, can make the sample portion 3 out that there is screw thread
Structure or flexible seal member, it is close so as to be screwed on by the sample-adding portion with device to be loaded or pass through seal member
Seal the interface section.
Above-mentioned helicitic texture can be internal thread structure or external thread structure, as long as being with device sealed connection to be loaded
It can.The flexible seal member can be rubber stopper etc., can specifically use sample portion and be arranged inside the rubber stopper, complete close
Envelope connection makes the injection port that device to be loaded is sealed by rubber stopper.
A preferred embodiment according to the present invention, the sample portion 3 out includes sample output passage 8, the sample output passage 8
Connect the sample export end of the sample cell and the sample portion 3 out.The sample output passage 8 is preferably arranged to tubulose, more preferably
Transparent tubulose.By the way that sample output passage 8 is arranged, sample feeding situation can be more easily observed.
According to the present invention, for the ease of the sample introduction situation of observation sample, the sample cell 2 is transparent or semitransparent sample
Pond, the sample cell lid 1 are transparent or semitransparent sample cell lid.As the sample cell 2 and the sample cell 1 material,
Such as one of glass, polypropylene, quartz, polymethyl acrylate and polystyrene or a variety of can be selected from.Wherein it is preferably
Glass, polymethyl acrylate or polypropylene.It, can be at any time by setting transparent or semitransparent for sample cell lid 1 and sample cell 2
The liquids and gases state in sample cell is grasped in observation, convenient for control sample-adding process.
It for the ease of the processing of sample, can be pre-processed during sample introduction, such as the bottom for stating sample cell 2 can be made
Portion is provided with sample processing element.Preferably, the sample processing element is sample filtering component, example enrichment component and sample
One of separation unit is a variety of, is specifically as follows one of filter disc, functional resin, silica gel and aluminium oxide or a variety of.Make
It can be hydrophobicity filter disc, such as polypropylene fibre, glass fibre, active carbon, gravel etc. for the filter disc.As the function
Resin, such as cation exchange resin, 18 alkyl silica gel etc..The setting quantity and set-up mode of above-mentioned sample processing element do not have
Have special restriction, can be one or more, and can according to need that the setting between each sample processing component needs every
The segmenting structures such as plate, to guarantee successively to carry out variety of processes.It, can be in sample introduction mistake by the way that above-mentioned sample processing element is arranged
The pretreatment that sample is carried out while journey, easily facilitates the progress of sample introduction and subsequent reactions.
Second aspect of the present invention provides a kind of micro-fluidic chip, and the micro-fluidic chip has injection port 5, the injection port
5 setting helicitic textures, the helicitic texture of the helicitic texture and above-mentioned manual injector match.Micro-fluidic chip of the invention
It can be used cooperatively with the manual injector of above-mentioned controllable sampling volume, the screw thread knot of manual injector is set during sample introduction
Structure is screwed on the helicitic texture on the injection port 5 that micro-fluidic chip is arranged in, to realize that the two is tightly connected, prevents sample introduction mistake
Pressure influence sampling volume in journey inside micro-fluidic chip.
In the present invention, there is no particular limitation for the structure of the micro-fluidic chip, preferably has reaction tank, and can have
There are the structures such as runner.The quantity of the reaction tank can be arranged according to quantity, can be more than one, such as 1-10, preferably
It is 1-2.The structure of the runner is configured according to the needs of reaction.
A preferred embodiment according to the present invention, such as micro-fluidic chip shown in Fig. 3, with injection port 5,
First reaction tank 6 and the second reaction tank 7, and between the injection port 5 and first reaction tank 6, first reaction
Be provided with runner between pond 6 and second reaction tank 7, be provided with internal screw thread at the injection port 5, so as to sample portion out
It cooperates with externally threaded hand sampling device and carries out sample introduction.
Third aspect present invention provides a kind of hand sampling method of controllable sampling volume, this method comprises: movement is above-mentioned
The sample cell lid 1 of manual injector extrudes the sample in the sample cavity by going out sample portion 3.The mobile sample cell lid 1
Mode can be rotation or pull etc., be carried out according to the structure of the manual injector.Pass through the movement sample cell
Lid 1, can be with the volume-diminished of sample cavity, and sample therein is discharged from manual injector under the effect of the pressure, to complete
Sample introduction process.
, according to the invention it is preferred to which the sample cell lid 1 is screwed on the sample cell 2, the mobile sample cell lid 1 passes through rotation
Turn the sample cell lid 1 to carry out.As above-mentioned, the process for rotating the sample cell lid 1 can be accurately controlled, so as to more
Accurately control injection volume.In order to guarantee the sealing between sample cell lid 1 and sample cell 2, such as can be in 1 He of sample cell lid
The threaded portion that sample cell 2 is screwed on carries out frosted processing.
A preferred embodiment according to the present invention, this method comprises: passing through the side wall that the sample cell 2 is arranged in
On scale determine by it is described go out the volume of sample that extrudes of sample portion 3.Sample volume during sample introduction can use the scale
It directly reads, thus the sample of volume needed for being more accurately loaded.
A preferred embodiment according to the present invention, this method comprises: by the manual injector with it is above-mentioned micro-
Fluidic chip is screwed on, and the sample in the manual injector is made to enter the micro-fluidic chip.By by manual injector with it is micro-
Fluidic chip is screwed on, and sample introduction process can be made more accurate and reliable.
The present invention also provides above-mentioned manual injector, micro-fluidic chip or hand sampling methods in environmental pollution quality testing
The application in the fields such as survey, in-vitro diagnosis, micro-total analysis chip.
The present invention will be described in detail by way of examples below.
Embodiment 1
The present embodiment is used to illustrate the sample injection of known volume.
Using the manual injector of controllable sampling volume as shown in Figs. 1-2, which includes sample cell 2, sample
Product pond lid 1 and out sample portion 3；The sample cell lid 1 is cylindrical, transparent glass material with the sample cell 2, and detachable
Ground be screwed on to be formed tube-in-tube structure to sealing formed volume be 100 μ L sample cavity, out sample portion 3 be located at the sample cell 2 with
The opposite side of the sample cell lid 1 can change the volume of the sample cavity by the movement of the sample cell lid 1, and will
Sample in the sample cavity is extruded by going out sample portion 3, and sample portion 3 includes straight tube-like sample output passage 8, the connection of sample output passage one end out
Sample cell, the other end are provided with external screw thread as outlet, and at the sample export end of outlet.It is set on the side wall of sample cell 2
It is equipped with 0~5 scale.
Using the micro-fluidic chip as shown in Figure 3 matched with above-mentioned manual injector, the micro-fluidic chip have into
Sample mouth 5, the first reaction tank 6 and the second reaction tank 7, and between the injection port 5 and first reaction tank 6, described
Be provided with runner between one reaction tank 6 and second reaction tank 7, be provided with internal screw thread at injection port 5, so as to sample out
The externally threaded mutual cooperation in portion 3 carries out sample introduction.
Before carry out process starts, it is first added the sample of 65 μ L in manual injector, then with 1 sealed sample pond of sample cell lid
2.Then the internal screw thread at the injection port 5 of the external screw thread and micro-fluidic chip in sample portion 3 out is screwed on, makes manual injector and miniflow
Chip connection is controlled, rotating sample pool lid 1 moves down sample cell lid 1, and sample cavity volume reduces, pressure increases, and sample is in the pressure
Effect is lower to fully enter micro-fluidic chip by sample output passage 8, can be just full of 6 He of the first reaction tank in micro-fluidic chip
Second reaction tank 7.During sample introduction, the stream of internal liquid is able to observe that by sample cell 2, sample cell lid 1 and sample output passage 8
Emotionally condition.
During sample cell 1 precession of lid, regardless of which position sample cell lid 1 reaches, it can suspend or stop at any time
Only sample introduction process, even if loosing one's grip, the position of sample cell lid can also be blocked by helicitic texture, without protecting there is a phenomenon where retracting
The stabilization for demonstrate,proving pressure in sample cell, so that the liquid stabilising in micro-fluidic chip, will not flow back.
Embodiment 2
The present embodiment is used to illustrate the sample injection of unknown volume.
Use manual injector and micro-fluidic chip same as Example 1.
Before carry out process starts, first it is added sample in manual injector, then with 1 sealed sample pond 2 of sample cell lid, and makes
Sample cell lid lower end is overlapped with 0 graduation mark of sample cell.Then by the injection port 5 of the external screw thread and micro-fluidic chip in sample portion 3 out
The internal screw thread at place is screwed on, and connect manual injector with micro-fluidic chip, and rotating sample pool lid 1 moves down sample cell lid 1, sample
Chamber volume reduces, pressure increases, and sample enters micro-fluidic chip by sample output passage 8 under pressure effect, sees in sample introduction
The reading for examining scale stops sample introduction when sample cell lid is overlapped with the scale 2 of sample cell, so as to add into micro-fluidic chip
Enter the sample of 65 μ L.During sample introduction, internal liquid is able to observe that by sample cell 2, sample cell lid 1 and sample output passage 8
Mobility status.
During sample cell 1 precession of lid, regardless of which position sample cell lid 1 reaches, it can suspend or stop at any time
Only sample introduction process, even if loosing one's grip, the position of sample cell lid can also be blocked by helicitic texture, without protecting there is a phenomenon where retracting
The stabilization for demonstrate,proving pressure in sample cell, so that the liquid stabilising in micro-fluidic chip, will not flow back.
The preferred embodiment of the present invention has been described above in detail, and still, the present invention is not limited thereto.In skill of the invention
In art conception range, can with various simple variants of the technical solution of the present invention are made, including each technical characteristic with it is any its
Its suitable method is combined, and it should also be regarded as the disclosure of the present invention for these simple variants and combination, is belonged to
Protection scope of the present invention.
Claims (14)
1. a kind of manual injector of controllable sampling volume, which is characterized in that the manual injector includes sample cell (2), sample
Chi Gai (1) and out sample portion (3)；
The sample cell lid (1) seals the sample cell (2) and forms sample cavity, and described sample portion (3) out are located at the sample cell (2)
The side opposite with the sample cell lid (1), can change the appearance of the sample cavity by the movement of the sample cell lid (1)
Product, and the sample in the sample cavity is extruded by going out sample portion (3).
2. manual injector according to claim 1, wherein it is provided with scale on the side wall of the sample cell (2), it is described
Scale is used to indicate the relative position of sample cell lid (1) Yu the sample cell (2).
3. manual injector according to claim 1, wherein the sample cell lid (1) and the sample cell (2) are detachable
Ground connection；
Preferably, the sample cell (2) and the sample cell lid (1) are cylindricality；
Preferably, the sample cell lid (1) is screwed on the sample cell (2).
4. manual injector according to claim 1, wherein it is described go out sample portion (3) setting the sample cell (2) with
The middle part of the opposite side of the sample cell lid (1).
5. manual injector according to claim 1, wherein described sample portion (3) out have helicitic texture or flexible sealing
Component；
Preferably, the helicitic texture is internal thread structure or external thread structure；
Preferably, the flexible seal member is rubber stopper.
6. manual injector according to claim 1, wherein sample portion (3) out include sample output passage (8).
7. manual injector described in any one of -6 according to claim 1, wherein the sample cell (2) is transparent or half
Transparent sample pond, the sample cell lid (1) are transparent or semitransparent sample cell lid.
8. manual injector described in any one of -6 according to claim 1, wherein the bottom of the sample cell (2) is arranged
There is sample processing element；
Preferably, the sample processing element is one of sample filtering component, example enrichment component and sample separation unit
Or it is a variety of.
9. a kind of micro-fluidic chip, which is characterized in that the micro-fluidic chip has injection port (5), injection port (5) setting
The helicitic texture of helicitic texture, manual injector described in any one of the helicitic texture and claim 1-8 matches.
10. a kind of hand sampling method of controllable sampling volume, which is characterized in that any one of mobile claim 1-8 institute
The sample cell lid (1) for the manual injector stated extrudes the sample in the sample cavity by going out sample portion (3).
11. hand sampling method according to claim 10, wherein the sample cell lid (1) and the sample cell (2) are revolved
It connects, the mobile sample cell lid (1) is carried out by rotating the sample cell lid (1).
12. hand sampling method according to claim 10, wherein this method comprises: by being arranged in the sample cell
(2) scale on side wall determines the volume by the sample that sample portion (3) extrude described out.
13. hand sampling method described in any one of 0-12 according to claim 1, wherein this method comprises: by the hand
Dynamic sample injector is screwed on micro-fluidic chip as claimed in claim 9, and the sample in the manual injector is made to enter the miniflow
Control chip.
14. manual injector described in any one of -9, the micro-fluidic chip in claim 9 or power according to claim 1
Benefit requires hand sampling method described in any one of 10-13 in environmental pollution analyte detection, in-vitro diagnosis or micro-total analysis core
The application in piece field.
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| CN201810448106.6A CN110470856A (en) | 2018-05-11 | 2018-05-11 | The manual injector and sample injection method of controllable sampling volume and its application |
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| CN201810448106.6A CN110470856A (en) | 2018-05-11 | 2018-05-11 | The manual injector and sample injection method of controllable sampling volume and its application |
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