CN207793273U - Multi-functional nucleic acid detection apparatus - Google Patents
Multi-functional nucleic acid detection apparatus Download PDFInfo
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- CN207793273U CN207793273U CN201721921013.8U CN201721921013U CN207793273U CN 207793273 U CN207793273 U CN 207793273U CN 201721921013 U CN201721921013 U CN 201721921013U CN 207793273 U CN207793273 U CN 207793273U
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Abstract
The utility model discloses a kind of multi-functional nucleic acid detection apparatus comprising pedestal, transmission mechanism, liquid sucting mechanism, aspirating mechanism and the shell with pedestal sealing.By the way that multiple liquid baths are arranged on pedestal, multiple functions can be gathered in one, such as can be used for the extraction, amplification and detection of nucleic acid, or the heavy target template of at least one extracted amplification and detection etc..The multi-functional nucleic acid detection apparatus is coordinated by pedestal and body seal, form an independent cavity, liquid sucting mechanism may be implemented between different liquid baths by transmission mechanism, liquid sucting mechanism and the aspirating mechanism entrained by manipulation device to switch, and imbibition or drain in different liquid baths may be implemented.The multi-functional nucleic acid structure of the detecting device is simple, small size can be achieved, multiple functions can be gathered in detection of nucleic acids in one, it is easy to operate, detection of nucleic acids can be made to be used in the medical environment for more lacking professional technician and experiment condition, be conducive to the application and popularization of detection of nucleic acids.
Description
Technical field
The utility model is related to molecular Biological Detection fields, more particularly, to a kind of multi-functional nucleic acid detection apparatus.
Background technology
Nucleic acid amplification detection has been widely used in the fields such as biological medicine.It is traditional in nucleic acid amplification detection process
In, the cross contamination of generally existing gene-amplification product and the case where cause the testing result of false positive.Also, traditional nucleic acid expands
Increase detection method used in template be required for greatly by independent sample extraction step, and the step for also easily cause template friendship
Fork pollution, influences the judgement of pattern detection result.There are some integrated nucleic acid detection apparatus, such as GeneXpert currently on the market
System etc. can realize extraction, amplification and detection to determined nucleic acid in a closed system, largely avoid
Pollution of the sample in extraction and amplification procedure effectively raises the accuracy and operability of detection of nucleic acids, but these are examined
Survey that device generally existing volume is big, construction is complicated, is both needed to using fluorescence probe and the high problem of testing cost, and this kind of detection dress
It is external development ＆ production to set, and equipment price is expensive；There are also the nucleic acid detection apparatus of miniaturization for other, but in detection process
In again there is a problem of that accuracy of detection is not high.
Utility model content
Based on this, it is necessary to provide it is a kind of it is simple in structure, occupy it is small and be conducive to improve accuracy of detection it is multi-functional
Nucleic acid detection apparatus.
The technical solution that the utility model solves above-mentioned technical problem is as follows.
A kind of multi-functional nucleic acid detection apparatus, including pedestal, transmission mechanism, liquid sucting mechanism, aspirating mechanism and with the bottom
The shell that seat is tightly connected；
Wherein, the pedestal is equipped with multiple liquid baths, and the pedestal matches to form airtight space with the shell, described
The open end of liquid bath is located in the airtight space；
The transmission mechanism is located on the top of the shell, and one end stretches out the shell and drives dress to external rotation
It sets, the transmission mechanism includes that transmission axis and bottom bracket axle, the bottom bracket axle are sleeved on the transmission axis, the transmission axis
It can be driven, and can be independently rotated by the rotating driving device with the bottom bracket axle, the transmission axis and described
Bottom bracket axle is respectively positioned in the airtight space；
The liquid sucting mechanism is located in the airtight space, and the liquid sucting mechanism is connect with the transmission mechanism, the biography
Dynamic central axis and bottom bracket axle rotation are respectively used for driving the liquid sucting mechanism and move up and down and rotate in vertical direction
Movement between different liquid baths to switch over；
The aspirating mechanism is connected to the liquid sucting mechanism, for making the liquid sucting mechanism draw liquid from the liquid bath
Or liquid is discharged to the liquid bath.
The liquid bath protrudes from the bottom of the pedestal backwards to the bottom of the shell in one of the embodiments, with
For being inserted into external equipment.
The liquid bath includes at least amplification slot and detect tank in one of the embodiments, and multiple liquid baths surround institute
State transmission mechanism setting.
The multi-functional nucleic acid detection apparatus further includes the survey being located in the airtight space in one of the embodiments,
Strip, the test-strips absorb the liquid in the detect tank close to the detect tank.
The transmission mechanism further includes central axis connector and axle sleeve rotation connection in one of the embodiments,
Head；The transmission axis is screw structure, and there is gap, the bottom bracket axle to be equipped between the bottom bracket axle and the transmission axis
Along the axially extending limited mouth of the transmission axis；The central axis connector is fixedly connected with the transmission axis, institute
Central axis connector is stated for being connect with rotating driving device to drive the transmission central axis；The axle sleeve rotation connection
Head is fixedly connected with the bottom bracket axle, and the axle sleeve rotation connection head with rotating driving device for connecting to drive the axis
Set rotation；
One end of the liquid sucting mechanism is sleeved on the transmission axis across the limited mouth and is passed with the transmission axis
Dynamic connection；When the transmission central axis, the liquid sucting mechanism can be driven to rise or fall；When the bottom bracket axle rotates
When, the inner wall of the bottom bracket axle can be abutted against with the liquid sucting mechanism to drive the liquid sucting mechanism to rotate.
The liquid sucting mechanism includes linking arm, imbibition cavity and imbibition tip in one of the embodiments,；The connection
One end of arm is connect across the limited mouth with the transmission middle axle driven, and the imbibition chamber body is located at the another of the linking arm
End, the imbibition cavity are connected to the imbibition tip, and the imbibition cavity is also connected to the aspirating mechanism.
Imbibition cavity described in the multi-functional nucleic acid detection apparatus includes imbibition slot and slot in one of the embodiments,
Lid, the capping sealing cover are located on the imbibition slot, and the capping is equipped with imbibition connecting pole, and the aspirating mechanism, which is equipped with, to be taken out
Inhale connecting pole, between the aspirating mechanism and the liquid sucting mechanism by both ends respectively with the suction connecting pole and the imbibition
The hose connection of connecting pole connection.
The aspirating mechanism has multiple pulsometers in one of the embodiments, and the volume of multiple pulsometers is not
With to form multistage suction system.
The shell is equipped with well in one of the embodiments, and the well corresponds at least one liquid bath,
The well is equipped with port lid.
The multi-functional nucleic acid detection apparatus further includes sealing plate in one of the embodiments, and the shell is close
The position of the well is equipped with sliding slot, and the sealing plate is nested on the sliding slot and can be along the sliding slot to the sample-adding
It unidirectionally moves in hole.
Above-mentioned multi-functional nucleic acid detection apparatus can contain various reactions by the way that multiple liquid baths are arranged on pedestal in liquid bath
Required reagent or container etc. as reaction or detection, thus can gather multiple functions in one, such as can be used for nucleic acid
Extraction, amplification and detection, or the amplification of at least one heavy target template and detection etc. that extract.The multi-functional detection of nucleic acids
Device is coordinated by pedestal and body seal, is formed an independent airtight space, is passed through the driver entrained by manipulation device
Structure, liquid sucting mechanism and aspirating mechanism may be implemented liquid sucting mechanism and switch between different liquid baths, and may be implemented different
Imbibition or drain, simple to operate in liquid bath.The multi-functional nucleic acid detection apparatus at least has the advantages that：
1. each imbibition, drain operation can automatically be carried out by external machinery equipment, can avoid artificially grasping when detection of nucleic acids
Make the operating error brought, is conducive to the stabilization of testing result；
2. being in direct contact clinical sample when avoidable manual operation, there is protection to make the life security of experiment operator
With；
3. can avoid the cross contamination between sample to be checked, be conducive to the confidence level for increasing pattern detection result；
4. whole operation process is substantially without manual operation, it is possible to reduce the direct participation of people is reduced to experimenter's skill
The requirement of art level；
5. the amplification and detection of nucleic acid can be completed directly in airtight space, avoids detection of nucleic acids and need in standard
The necessary condition that PCR Lab is completed, reduces the threshold and cost of detection of nucleic acids.
The utility model by nucleic acid extraction and detection reaction process in each influence factor carry out researching and analysing discovery,
How to ensure that the stable gas pressure in airtight space has important influence, above-mentioned multifunctional core for the precision of imbibition and drain
The transmission axis and bottom bracket axle and liquid sucting mechanism of the transmission mechanism of sour detection device are all located in airtight space, in liquid sucting mechanism
When rotation and upper and lower movement, since airtight space volume does not change substantially, air pressure in airtight space will not be caused
Variation, thereby may be ensured that the stability of air pressure in airtight space, and then liquid sucting mechanism can be ensured in imbibition or drain
Precision is conducive to the accuracy and reliability for improving testing result not by effects of air pressure.
Liquid sucting mechanism is driven to move above and below respectively by the rotation of transmission axis and bottom bracket axle inside airtight space
And rotation can be to avoid using the sealing structures such as many sealing rings in transmission mechanism and shell contact site when device is assembled
To form the trouble effectively sealed, structure design is simpler, and external rotating driving device driving effect is more preferable, rotary motion
Precision is easier control.
The multi-functional nucleic acid detection apparatus is mainly disposable product, as disposable consumptive material, structure
It is simple to gather multiple functions in detection of nucleic acids in one, it can be achieved that small size, it is easy to operate, detection of nucleic acids can be made
It is used in the case where more lacking the medical environment of professional technician and experiment condition, is conducive to the application and popularization of detection of nucleic acids.
Description of the drawings
Fig. 1 is the structural schematic diagram of the multi-functional nucleic acid detection apparatus of an embodiment；
Fig. 2 is the structural schematic diagram at another visual angle of multi-functional nucleic acid detection apparatus shown in Fig. 1；
Fig. 3 is the decomposed schematic diagram of multi-functional nucleic acid detection apparatus shown in Fig. 1；
Fig. 4 is the structural schematic diagram of pedestal in Fig. 3；
Fig. 5 is the structural schematic diagram of shell in Fig. 3；
Fig. 6 is the assembling schematic diagram of the other structures in addition to decapsidate in Fig. 3；
Fig. 7 is the structural schematic diagram of transmission mechanism in Fig. 6；
Fig. 8 is the structural schematic diagram of liquid sucting mechanism in Fig. 6；
Fig. 9 is the structural schematic diagram of aspirating mechanism in Fig. 6.
Specific implementation mode
The utility model is more fully retouched below with reference to relevant drawings for the ease of understanding the utility model,
It states.The preferred embodiment of the utility model is given in attached drawing.But the utility model can in many different forms come in fact
It is existing, however it is not limited to embodiment described herein.Make public affairs to the utility model on the contrary, purpose of providing these embodiments is
The understanding for opening content is more thorough and comprehensive.
It should be noted that when element is referred to as " being fixed on " another element, it can be directly on another element
Or there may also be elements placed in the middle.When an element is considered as " connection " another element, it can be directly connected to
To another element or it may be simultaneously present centering elements.
Unless otherwise defined, all of technologies and scientific terms used here by the article is led with the technology for belonging to the utility model
The normally understood meaning of technical staff in domain is identical.Terminology used in the description of the utility model herein only be
The purpose of description specific embodiment, it is not intended that in limitation the utility model.
Incorporated by reference to Fig. 1, Fig. 2 and Fig. 3, the multi-functional nucleic acid detection apparatus 10 of an embodiment includes pedestal 100, shell
200, transmission mechanism 300, liquid sucting mechanism 400 and aspirating mechanism 500.Pedestal 100 is tightly connected with shell 200, and the two matches
Form airtight space.
Incorporated by reference to Fig. 3, Fig. 4 and Fig. 5, in the present embodiment, pedestal 100 is equipped with multiple liquid baths, and the opening of liquid bath
End is located in the airtight space.Multiple liquid baths can be used for a variety of different functions, reservoir can be such as used as, to store nucleic acid
Reagent (such as lysate and eluent etc.) needed for extraction, the reagent needed for PCR amplification (such as primer kit, PCR buffer solutions, enzyme,
DNTPs etc.) or detection needed for reagent (such as probe, enzyme, fluorescent reagent) etc.；It can be for another example used as extraction tank, for carrying
Take or elute sample of nucleic acid；It can be for another example used as amplification slot, for PCR amplification；It can be for another example used as detect tank, for containing
Reaction solution etc. of the dress for detection；It can be for another example used as waste liquid tank, to contain waste liquid in reaction process etc..The number of all kinds of liquid baths
Measure it is either one or more, such as in a specific embodiment, the liquid bath includes reservoir 101, extraction tank
102, slot 103, detect tank 104 and waste liquid tank 105 are expanded；For another example in another specific embodiment, the liquid bath only includes
Expand slot 103 and detect tank 104.
Pedestal 100 is substantially in the form of annular discs.Each liquid bath may be designed to different size and shape, while arrange it as needed
Position, such as in one embodiment, reservoir 101 can be designed to different shape and depth according to actual needs.One
In a specific embodiment, multiple liquid baths are arranged around the edge of pedestal 100, such as can form a circle in arcuation.Further,
When storage has reagent in liquid bath, the notch of liquid bath is sealed with the diaphragm seal that can be punctured.Diaphragm seal can be but not limited to aluminium foil
Paper etc., in order to the storage of reagent and the transport of whole device.
In one embodiment, liquid bath protrudes from the bottom of pedestal 100 backwards to the bottom of shell 200, for insertion into outer
It connects in equipment.Correspondingly, the base unit of external equipment has multiple slot positions, for for different liquid bath insertions.It is external to set
Heating module, the supplementary module of detection or magnet module can be arranged (as being used for magnetic in each slot position of correspondence in standby base unit
Power stirs) etc., to coordinate each function liquid bath to execute corresponding function.
Incorporated by reference to Fig. 3, Fig. 4 and Fig. 5, shell 200 is substantially in hood-like structure.Match with the sealing of pedestal 100 200 bottom of shell
It closes, the mode that can be such as connected through a screw thread is connected, can also be connected by way of clamping.Between shell 200 and pedestal 100
It can be through but not limited to sealing structures such as sealing rings.The top of shell 200 has multiple operative positions, such as transmission position 201 and pumping
Inhale position 202 etc., wherein transmission position 201 corresponds to transmission mechanism 300, and suction position 202 corresponds to aspirating mechanism 500.
In one embodiment, it is additionally provided with well 203 on shell 200.Well 203 corresponds at least one liquid of lower section
Slot, for sample to be tested is added into the liquid bath, such as in a specific embodiment, well 203 can be located at extraction tank
102 surface or positioned at amplification slot 103 surface, with the sample that nucleic acid to be extracted is added or extracted sample of nucleic acid
Deng.Further, it is correspondingly provided with port lid 204 at the well 203.Port lid 204 is outer to ensure to make for sealing well 203
The space-closed that shell 200 is formed with pedestal 100.
Further, in a specific embodiment, which further includes sealing plate 205.Outside
Shell 200 is being equipped with sliding slot 206 close to the position of the well 203, and sealing plate 205 is nested on sliding slot 206 and can be along cunning
Slot 206 is unidirectionally mobile to well 203, and port lid 204 is pressed on well 203, prevent when operation port lid 204 unclamp or
It falls off, is further ensured that the air-tightness of inner space.The unidirectional mobile sealing performance that may further ensure that sealing plate 205, with
It prevents maloperation or occurs the case where the disengaging of sealing plate 205 sealing station caused by the extraneous factors such as vibration.
As shown in Figure 6 and Figure 7, transmission mechanism 300 is located at the top of shell 200, and one end is stretched out other than the use of shell 200
Connect rotating driving device.Specifically, in one embodiment, one end of transmission mechanism 300 corresponds to the transmission position on shell 200
201 settings.
In diagrammatically shown specific embodiment, transmission mechanism 300 be jacket type structure, including transmission axis 310, in
Axle sleeve 320, central axis connector 330 and axle sleeve are rotatablely connected head 340.Transmission axis 310 is screw structure, bottom bracket axle
320 are sleeved on transmission axis 310 and have gap between transmission axis 310.Bottom bracket axle 320 is equipped with along transmission axis 310
Axially extending limited mouth 322.It is driven axis 310 and independent rotation is driven by independent connector with bottom bracket axle 320, specifically,
Central axis connector 330 is fixedly connected with transmission axis 310, and central axis connector 330 is used to connect with rotating driving device
It connects to drive transmission axis 310 to rotate；Axle sleeve rotation connection head 340 is fixedly connected with bottom bracket axle 320, and axle sleeve is rotatablely connected head
340 with rotating driving device for connecting to drive bottom bracket axle 320 to rotate.
Incorporated by reference to Fig. 6, Fig. 7 and Fig. 8, liquid sucting mechanism 400 is connect with transmission mechanism 300, and liquid sucting mechanism 400 is located at described
In airtight space, and rotation can be driven to be switched between different liquid baths by transmission mechanism 300, or in vertical direction
On move up and down.
Specifically, in one embodiment, one end of liquid sucting mechanism 400 is sleeved on across limited mouth 322 on transmission axis 310
And it is sequentially connected (being threadedly coupled) with transmission axis 310.When being driven the rotation of axis 310, it can drive and be arranged on it
Liquid sucting mechanism 400 rises or falls；When bottom bracket axle 320 rotates, since the inner wall of bottom bracket axle 320 can be with liquid sucting mechanism 400
It abuts against, and then being capable of the rotation of synchronous drive liquid sucting mechanism 400.
More specifically, in diagrammatically shown embodiment, liquid sucting mechanism 400 includes linking arm 410, imbibition cavity 420 and inhales
Liquid tip 430.One end of linking arm 410 passes through limited mouth 322 to be sequentially connected with transmission axis 310.Imbibition cavity 420, which is located at, to be connected
The other end of arm 410 is connect, imbibition cavity 420 is connected to imbibition tip 430.
Incorporated by reference to Fig. 6, Fig. 8 and Fig. 9, aspirating mechanism 500 is connected to imbibition cavity 420, with by aspirating mechanism 500 drive from
Liquid bath draws liquid or liquid is discharged to liquid bath.
Specifically, in one embodiment, aspirating mechanism 500 has multiple pulsometers 510.Pulsometer 510 is plunger type
Pump body structure, by aspirate or push action imbibition or drain may be implemented.The volume difference of multiple pulsometers 510 is with shape
Ensure precision when absorption or drain at multistage suction system to draw or exclude different amounts of liquid.
Further, in diagrammatically shown embodiment, imbibition cavity 420 includes imbibition slot 421 and capping 422.Capping 422 is close
Capping is located on imbibition slot 421.Capping 422 is equipped with imbibition connecting pole 423, and imbibition connecting pole 423 connects with imbibition cavity 420
It is logical.Correspondingly, aspirating mechanism 500 is equipped with suction connecting pole 520.Multiple pulsometers 510 are located on the suction connecting pole 520 and each
It is connected to from suction connecting pole 520.Between aspirating mechanism 500 and liquid sucting mechanism 400 by both ends respectively with suction connecting pole
520 and imbibition connecting pole 423 be connected to hose (not shown) connection.Hose has certain length, to ensure liquid sucting mechanism 400
In rotation or lifting with certain freedom of movement.
It please join Fig. 3 and Fig. 4, in a specific embodiment, which further includes being located at gas
Test-strips 600 in close space.Test-strips 600 absorb the liquid in the liquid bath close to the liquid bath of detection.To inspection
After liquid is added in the liquid bath of survey, with the generation of chromatography phenomenon, component and 600 corresponding site of test-strips in amplified production
Group split-phase hybridization, and then form coloured band.The band can be detected by the detection module in external equipment, and then be formed
Sentence read result.It is understood that in other embodiments, which can also use other detection modes,
Fluoroscopic examination is such as carried out, correspondingly, the bottom of 100 corresponding detect tank 104 of pedestal is set as transparent.
In diagrammatically shown specific embodiment, when in use, user can be first for the multi-functional nucleic acid detection apparatus 10
Port lid 204 is opened, the sample or pre- of certain volume is added into the extraction tank 102 for be stored with sample lysate using sample needle
Sample is handled, is then covered port lid 204 on well 203, and sealing plate 205 is made to slide on well 203.By more work(
Energy nucleic acid detection apparatus 10 is put into the base unit of external equipment.After multi-functional nucleic acid detection apparatus 10 is installed, transmission mechanism
300, aspirating mechanism 500 etc. matches machinery corresponding with external equipment, and adding for nucleic acid amplification is useful in base unit
The structures such as thermal modules, the supplementary module of detection and transportable magnet.Now by taking the sample extracted as an example, to more work(
Specifically amplification and detecting step are illustrated 10 internal operation of energy nucleic acid detection apparatus, specific as follows：
1) imbibition tip 430 is gone to the surface of extraction tank 102 by rotating middle shaft set 320, and by base unit
Alignment sensor verifies the movement；
2) air in the push rod discharge imbibition tip 430 of pressure pulsometer 510, while transmission axis 310 is rotated, lead to
Screw structure is crossed to drive imbibition tip 430 to be moved downwardly into extraction tank 102 and make the tip of imbibition tip 430 to extraction tank
Below 102 liquid level；
3) push rod is suitably unclamped, the recovery spring in pulsometer 510 increases, and sample solution is made to flow into imbibition tip 430；
4) rotation transmission axis 310, drives imbibition tip 430 to move upwardly off extraction tank 102；
5) imbibition tip 430 is gone to amplification slot 103 by rotating middle shaft set 320, using the alignment sensor in base unit
Verify the movement；
6) rotation transmission axis 310 drives imbibition tip 430 to move down the sealing at the notch for puncturing amplification slot 103
Film into filling the amplification slot 103 of amplification system, and makes the tip to liquid level of imbibition tip 430 or less；
7) liquid in pressure push rod discharge imbibition tip 430；
8) rotation transmission axis 310 drives imbibition tip 430 to move upwardly off amplification slot 103；
9) rotating middle shaft set 320 rotates imbibition tip 430 to the top for the reservoir 101 for storing the mineral oil such as glycerine
The movement is verified in position using the alignment sensor of base unit；
10) rotation transmission axis 310, drives imbibition tip 430 to move down the sealing at the notch for puncturing reservoir 101
Film, into and make the tip to liquid level of imbibition tip 430 or less；
11) push rod is suitably unclamped, restoring spring raising makes mineral oil flow into imbibition tip 430；
12) rotation transmission axis 310, drives imbibition tip 430 to move upwardly off reservoir 101；
13) 320 rotation imbibition tip 430 of rotating middle shaft set is to the top position of amplification slot 103, using determining for base unit
Level sensor verifies the movement；
14) rotation transmission axis 310 drives imbibition tip 430 to be moved downwardly into amplification slot 103 and makes imbibition tip
Below 430 tip to liquid level.
15) liquid of pressure push rod discharge imbibition tip 430；
16) rotation transmission axis 310 drives imbibition tip 430 to move upwardly off amplification slot 103.
17) base unit carries out the program of nucleic acid amplification；
18) it is room temperature that base unit, which keeps the temperature of amplification slot 103,；
19) rotation transmission axis 310 drives imbibition tip 430 to be moved downwardly into amplification slot 103 and makes tip to liquid level
Below；
20) push rod is suitably unclamped, restoring spring raising makes amplified production flow into imbibition tip 430；
21) rotation transmission axis 310 drives imbibition tip 430 to move upwardly off amplification slot 103；
22) 320 rotation imbibition tip 430 of rotating middle shaft set is to the top position of detect tank 104, using determining for base unit
Level sensor verifies the movement；
23) rotation transmission axis 310, drives imbibition tip 430 to move down the sealing at the notch for puncturing detect tank 104
Film, into and make tip to liquid level or less；
24) liquid of pressure push rod discharge imbibition tip 430；
25) rotation transmission axis 310, drives imbibition tip 430 to move upwardly off detect tank 104；
26) test-strips 600 of the detect tank 104 equipped with detection amplified production, after the liquid addition, with the hair of chromatography phenomenon
Raw, component hybridizes with the group split-phase of test-strips corresponding site in amplified production, and then forms coloured band, which can
It is captured by base unit detection module, forms sentence read result.
Some above-mentioned steps can carry out more than primary.In addition, it is appreciated that in other embodiments, be not limited to according to upper
State step execution.
Above-mentioned multi-functional nucleic acid detection apparatus 10 can be contained each by the way that multiple liquid baths are arranged on pedestal 100 in liquid bath
Reagent or the container etc. as reaction or detection needed for kind reaction, thus multiple functions can be gathered in one, it can such as use
In the extraction, amplification and detection of nucleic acid, or the amplification of at least one heavy target template and detection etc. that extract.The multifunctional core
Sour detection device 10 is coordinated by pedestal 100 and the sealing of shell 200, is formed an independent cavity, is taken by manipulation device
Liquid sucting mechanism 400 may be implemented between different liquid baths in transmission mechanism 300, liquid sucting mechanism 400 and the aspirating mechanism 500 of band
Switching, and imbibition or drain in different liquid baths may be implemented, it is simple to operate.
The transmission axis 310 and bottom bracket axle 320 and imbibition machine of the transmission mechanism 300 of the multi-functional nucleic acid detection apparatus 10
Structure 400 is all located in airtight space, in the rotation of liquid sucting mechanism 400 and upper and lower movement, since airtight space volume does not have substantially
Variation, therefore, will not lead to the variation of air pressure in airtight space, thereby may be ensured that the stability of air pressure in airtight space, into
And can ensure precision of the liquid sucting mechanism 400 in imbibition or drain not by effects of air pressure, be conducive to the standard for improving testing result
True property and reliability.And imbibition is driven by the rotation of transmission axis 310 and bottom bracket axle 320 inside airtight space respectively
Movement and the rotation above and below of mechanism 400, when device assembles, can be saved in transmission mechanism 300 and 200 contact site of shell needs
Using sealing structures such as many sealing rings to form the trouble effectively sealed, structure design is simpler, and external rotation driving dress
It sets and drives effect more preferable, the precision of rotary motion is easier control.
The multi-functional nucleic acid detection apparatus 10 is mainly disposable product, as disposable consumptive material, knot
Structure is simple, it can be achieved that small size, multiple functions can be gathered in detection of nucleic acids in one, easy to operate, nucleic acid can be made to examine
It surveys and is used in the medical environment for more lacking professional technician and experiment condition, be conducive to the application and popularization of detection of nucleic acids.
Each technical characteristic of embodiment described above can be combined arbitrarily, to keep description succinct, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, it is all considered to be the range of this specification record.
Above-described embodiments merely represent several embodiments of the utility model, the description thereof is more specific and detailed,
But therefore it can not be interpreted as the limitation to utility model patent range.It should be pointed out that for the common skill of this field
For art personnel, without departing from the concept of the premise utility, various modifications and improvements can be made, these are belonged to
The scope of protection of the utility model.Therefore, the protection domain of the utility model patent should be determined by the appended claims.
Claims (10)
1. a kind of multi-functional nucleic acid detection apparatus, which is characterized in that including pedestal, transmission mechanism, liquid sucting mechanism, aspirating mechanism and
The shell being tightly connected with the pedestal；
Wherein, the pedestal is equipped with multiple liquid baths, and the pedestal matches to form airtight space with the shell, the liquid bath
Open end be located in the airtight space；
The transmission mechanism is located on the top of the shell, and the shell is stretched out to external rotating driving device in one end,
The transmission mechanism include transmission axis and bottom bracket axle, the bottom bracket axle be sleeved on the transmission axis, the transmission axis and
The bottom bracket axle can be driven by the rotating driving device, and can independently be rotated, the transmission axis and it is described in
Axle sleeve is respectively positioned in the airtight space；
The liquid sucting mechanism is located in the airtight space, and the liquid sucting mechanism is connect with the transmission mechanism, in the transmission
Shaft rotation is dynamic and bottom bracket axle rotation is respectively used for driving the liquid sucting mechanism and is moved up and down and rotary motion in vertical direction
To be switched between different liquid baths；
The aspirating mechanism is connected to the liquid sucting mechanism, for make the liquid sucting mechanism from the liquid bath draw liquid or to
Liquid is discharged in the liquid bath.
2. multi-functional nucleic acid detection apparatus as described in claim 1, which is characterized in that the liquid bath is backwards to the bottom of the shell
Portion protrudes from the bottom of the pedestal, for insertion into external equipment.
3. multi-functional nucleic acid detection apparatus as described in claim 1, which is characterized in that the liquid bath include at least amplification slot and
Detect tank, multiple liquid baths are arranged around the transmission mechanism.
4. multi-functional nucleic acid detection apparatus as claimed in claim 3, which is characterized in that further include being located in the airtight space
Test-strips, the test-strips absorb the liquid in the detect tank close to the detect tank.
5. multi-functional nucleic acid detection apparatus as described in claim 1, which is characterized in that the transmission mechanism further includes middle shaft rotation
Dynamic connector and axle sleeve are rotatablely connected head；The transmission axis is screw structure, the bottom bracket axle and the transmission axis it
Between have gap, the bottom bracket axle be equipped with along it is described transmission axis axially extending limited mouth；The central axis connector
It is fixedly connected with the transmission axis, the central axis connector with rotating driving device for connecting to drive the transmission
Central axis；The axle sleeve rotation connection head is fixedly connected with the bottom bracket axle, and the axle sleeve rotation connection head is used for and rotation
Driving device connection is to drive the bottom bracket axle to rotate；
One end of the liquid sucting mechanism is sleeved on the transmission axis across the limited mouth and connects with the transmission middle axle driven
It connects；When the transmission central axis, the liquid sucting mechanism can be driven to rise or fall；When the bottom bracket axle rotates, institute
Stating the inner wall of bottom bracket axle can abut against with the liquid sucting mechanism to drive the liquid sucting mechanism to rotate.
6. multi-functional nucleic acid detection apparatus as claimed in claim 5, which is characterized in that the liquid sucting mechanism include linking arm,
Imbibition cavity and imbibition tip；One end of the linking arm is connect across the limited mouth with the transmission middle axle driven, described
Imbibition chamber body is located at the other end of the linking arm, and the imbibition cavity is connected to the imbibition tip, and the imbibition cavity is also
It is connected to the aspirating mechanism.
7. multi-functional nucleic acid detection apparatus as claimed in claim 6, which is characterized in that the imbibition cavity include imbibition slot and
Capping, the capping sealing cover are located on the imbibition slot, and the capping is equipped with imbibition connecting pole, and the aspirating mechanism is equipped with
Aspirate connecting pole, between the aspirating mechanism and the liquid sucting mechanism by both ends respectively with the suction connecting pole and the suction
The hose connection of liquid connecting pole connection.
8. such as multi-functional nucleic acid detection apparatus according to any one of claims 1 to 7, which is characterized in that the aspirating mechanism
With multiple pulsometers, the volume difference of multiple pulsometers is to form multistage suction system.
9. such as multi-functional nucleic acid detection apparatus according to any one of claims 1 to 7, which is characterized in that the shell is equipped with
Well, the well correspond at least one liquid bath, and the well is equipped with port lid.
10. multi-functional nucleic acid detection apparatus as claimed in claim 9, which is characterized in that further include sealing plate, the shell exists
It is equipped with sliding slot close to the position of the well, the sealing plate is nested on the sliding slot and can be along the sliding slot to described
Well unidirectionally moves.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107964505A (en) * | 2017-12-28 | 2018-04-27 | 广州市宝创生物技术有限公司 | Multi-functional nucleic acid detection apparatus |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107964505A (en) * | 2017-12-28 | 2018-04-27 | 广州市宝创生物技术有限公司 | Multi-functional nucleic acid detection apparatus |
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