CN107964505A - Multi-functional nucleic acid detection apparatus - Google Patents
Multi-functional nucleic acid detection apparatus Download PDFInfo
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- 238000001514 detection method Methods 0.000 title claims abstract description 80
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 67
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 66
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 66
- 239000007788 liquid Substances 0.000 claims abstract description 124
- 230000007246 mechanism Effects 0.000 claims abstract description 102
- 230000005540 biological transmission Effects 0.000 claims abstract description 83
- 238000005213 imbibition Methods 0.000 claims abstract description 71
- 230000003321 amplification Effects 0.000 claims abstract description 29
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 29
- 238000007789 sealing Methods 0.000 claims abstract description 25
- 230000033001 locomotion Effects 0.000 claims description 12
- 238000003780 insertion Methods 0.000 claims description 2
- 230000037431 insertion Effects 0.000 claims description 2
- 238000000605 extraction Methods 0.000 abstract description 16
- 238000002474 experimental method Methods 0.000 abstract description 4
- 239000000523 sample Substances 0.000 description 15
- 238000010586 diagram Methods 0.000 description 9
- 238000006243 chemical reaction Methods 0.000 description 7
- 239000003153 chemical reaction reagent Substances 0.000 description 7
- 230000008859 change Effects 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 230000000994 depressogenic effect Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 206010061688 Barotrauma Diseases 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 238000012864 cross contamination Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 239000006166 lysate Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000002480 mineral oil Substances 0.000 description 2
- 235000010446 mineral oil Nutrition 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 230000004544 DNA amplification Effects 0.000 description 1
- 239000012445 acidic reagent Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000005030 aluminium foil Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of multi-functional nucleic acid detection apparatus, it includes base, transmission mechanism, liquid sucting mechanism, aspirating mechanism and the shell with base sealing.By setting multiple liquid baths on base, multiple functions can be gathered in one, such as can be used for the extraction, amplification and detection of nucleic acid, or the amplification and detection etc. of the heavy target template of at least one extracted.The multi-functional nucleic acid detection apparatus is coordinated by base and body seal, form an independent cavity, it can realize that liquid sucting mechanism switches between different liquid baths by the transmission mechanism entrained by manipulation device, liquid sucting mechanism and aspirating mechanism, and can realize imbibition or discharge opeing in different liquid baths.The multi-functional nucleic acid structure of the detecting device is simple, small size can be achieved, multiple functions can be gathered in detection of nucleic acids in one, it is easy to operate, detection of nucleic acids can be made to be used in the medical environment for more lacking professional technician and experiment condition, be conducive to the application and popularization of detection of nucleic acids.
Description
Technical field
The present invention relates to molecular Biological Detection field, more particularly, to a kind of multi-functional nucleic acid detection apparatus.
Background technology
Nucleic acid amplification detection is had been widely used in the fields such as biological medicine.It is traditional in nucleic acid amplification detection process
In, the cross contamination of generally existing gene-amplification product and cause the situation of the testing result of false positive.Also, traditional nucleic acid expands
Increase detection method used in template be required for greatly by independent sample extraction step, and the step for also easily cause template friendship
Fork pollution, influences the judgement of pattern detection result.There are some integrated nucleic acid detection apparatus, such as GeneXpert currently on the market
System etc., it can realize extraction, amplification and the detection to determined nucleic acid in the system of a closing, largely avoid
Pollution of the sample in extraction and amplification procedure, effectively raises the accuracy and operability of detection of nucleic acids, but these are examined
The problem of surveying big device generally existing volume, construction complexity, being both needed to use fluorescence probe and testing cost high, and this kind of detection dress
It is external development ＆ production to put, and equipment price is expensive；Other also have some nucleic acid detection apparatus minimized, but in detection process
In again there are accuracy of detection it is not high the problem of.
The content of the invention
Based on this, it is necessary to provide it is a kind of it is simple in structure, take it is small and be conducive to improve accuracy of detection it is multi-functional
Nucleic acid detection apparatus.
The technical solution that the present invention solves above-mentioned technical problem is as follows.
A kind of multi-functional nucleic acid detection apparatus, including base, transmission mechanism, liquid sucting mechanism, aspirating mechanism and with the bottom
The shell that seat is tightly connected；
Wherein, the base is equipped with multiple liquid baths, and the base is engaged to form airtight space with the shell, described
The openend of liquid bath is located in the airtight space；
The transmission mechanism is located on the top of the shell, and one end is stretched out the shell and filled to external rotation driving
Put, the transmission mechanism includes transmission axis and bottom bracket axle, and the bottom bracket axle is sleeved on the transmission axis, the transmission axis
It can be driven, and can be independently rotated by the rotating driving device with the bottom bracket axle, the transmission axis and described
Bottom bracket axle is respectively positioned in the airtight space；
The liquid sucting mechanism is located in the airtight space, and the liquid sucting mechanism is connected with the transmission mechanism, the biography
Dynamic central axis and bottom bracket axle rotation are respectively used for driving the liquid sucting mechanism and move up and down and rotate in vertical direction
Move to be switched between different liquid baths；
The aspirating mechanism is connected with the liquid sucting mechanism, for making the liquid sucting mechanism draw liquid from the liquid bath
Or discharge liquid to the liquid bath.
In one of the embodiments, the liquid bath protrudes from the bottom of the base backwards to the bottom of the shell, with
For being inserted into external equipment.
In one of the embodiments, the liquid bath includes at least amplification groove and detect tank, multiple liquid baths surround institute
State transmission mechanism setting.
In one of the embodiments, the multi-functional nucleic acid detection apparatus further includes the survey being located in the airtight space
Strip, the test-strips are close to the detect tank for the liquid in the absorption detect tank.
In one of the embodiments, the transmission mechanism further includes central axis connector and axle sleeve rotation connection
Head；The transmission axis is screw structure, has gap between the bottom bracket axle and the transmission axis, the bottom bracket axle is equipped with
Along the axially extending limited mouth of the transmission axis；The central axis connector is fixedly connected with the transmission axis, institute
Central axis connector is stated to be used to be connected with rotating driving device to drive the transmission central axis；The axle sleeve rotation connection
Head is fixedly connected with the bottom bracket axle, and the axle sleeve rotation connection head is used to be connected with rotating driving device to drive the axis
Set rotates；
One end of the liquid sucting mechanism is sleeved on the transmission axis through the limited mouth and is passed with the transmission axis
Dynamic connection；When the transmission central axis, the liquid sucting mechanism can be driven to rise or fall；When the bottom bracket axle rotates
When, the inner wall of the bottom bracket axle can be abutted against with the liquid sucting mechanism to drive the liquid sucting mechanism to rotate.
In one of the embodiments, the liquid sucting mechanism includes linking arm, imbibition cavity and imbibition tip；The connection
One end of arm is connected through the limited mouth with the transmission middle axle driven, and the imbibition chamber body is located at the another of the linking arm
End, the imbibition cavity are connected with the imbibition tip, and the imbibition cavity is also connected with the aspirating mechanism.
In one of the embodiments, imbibition cavity described in the multi-functional nucleic acid detection apparatus includes imbibition groove and groove
Lid, the capping sealing cover are located on the imbibition groove, and the capping is equipped with imbibition connecting pole, and the aspirating mechanism, which is equipped with, to be taken out
Connecting pole is inhaled, connecting pole and the imbibition are aspirated with described respectively by both ends between the aspirating mechanism and the liquid sucting mechanism
The hose connection of connecting pole connection.
In one of the embodiments, the aspirating mechanism has multiple pulsometers, and the volume of multiple pulsometers is not
With with the multistage suction system of formation.
In one of the embodiments, the shell is equipped with well, and the well corresponds at least one liquid bath,
The well is equipped with port lid.
In one of the embodiments, the multi-functional nucleic acid detection apparatus further includes sealing plate, and the shell is close
The position of the well is equipped with sliding slot, and the sealing plate is nested on the sliding slot and can be along the sliding slot to the sample-adding
Unidirectionally move in hole.
Above-mentioned multi-functional nucleic acid detection apparatus can contain various reactions by setting multiple liquid baths on base in liquid bath
Required reagent or container etc. as reaction or detection, thus multiple functions can be gathered in one, it such as can be used for nucleic acid
Extraction, amplification and detection, or the amplification and detection etc. of at least one heavy target template extracted.The multi-functional detection of nucleic acids
Device is coordinated by base and body seal, is formed an independent airtight space, is passed through the driver entrained by manipulation device
Structure, liquid sucting mechanism and aspirating mechanism can realize that liquid sucting mechanism switches between different liquid baths, and can realize different
Imbibition or discharge opeing, simple to operate in liquid bath.The multi-functional nucleic acid detection apparatus at least has the advantages that：
1. each imbibition, discharge opeing operation can be carried out automatically by external machinery equipment, can avoid artificially grasping during detection of nucleic acids
Make the operating error brought, beneficial to the stabilization of testing result；
2. can avoid directly contacting clinical sample during manual operation, there is protection to make the life security of experiment operator
With；
3. the cross contamination between sample to be checked can be avoided, be conducive to increase the confidence level of pattern detection result；
4. whole operation process is substantially without manual operation, it is possible to reduce people's directly participates in, and reduces to experimenter's skill
The requirement of art level；
5. the amplification and detection of nucleic acid can be completed directly in airtight space, avoiding detection of nucleic acids needs in standard
The necessary condition that PCR Lab is completed, reduces the threshold and cost of detection of nucleic acids.
The present invention by nucleic acid extraction and detection reaction process in each influence factor carry out researching and analysing discovery, how
Ensure that the stable gas pressure in airtight space has important influence, above-mentioned multi-functional nucleic acid inspection for the precision of imbibition and discharge opeing
The transmission axis and bottom bracket axle and liquid sucting mechanism of the transmission mechanism of survey device are all located in airtight space, are rotated in liquid sucting mechanism
During with upper and lower movement, since airtight space volume does not change substantially, the change of air pressure in airtight space will not be caused,
It thereby may be ensured that the stability of air pressure in airtight space, and then can ensure precision of the liquid sucting mechanism in imbibition or discharge opeing not
By effects of air pressure, be conducive to improve the accuracy and reliability of testing result.
The upper and lower movement of liquid sucting mechanism is driven by the rotation of transmission axis and bottom bracket axle inside airtight space respectively
And rotation, can be to avoid sealing structures such as many sealing rings of use in transmission mechanism and shell contact site when device assembles
To form the trouble effectively sealed, structure design is simpler, and external rotating driving device driving effect is more preferable, rotary motion
Precision is easier control.
The multi-functional nucleic acid detection apparatus is mainly disposable product, as disposable consumptive material, its structure
It is simple to gather multiple functions in detection of nucleic acids in one, it can be achieved that small size, it is easy to operate, detection of nucleic acids can be made
Used in the case where more lacking the medical environment of professional technician and experiment condition, be conducive to the application and popularization of detection of nucleic acids.
Brief description of the drawings
Fig. 1 is the structure diagram of the multi-functional nucleic acid detection apparatus of an embodiment；
Fig. 2 is the structure diagram at another visual angle of multi-functional nucleic acid detection apparatus shown in Fig. 1；
Fig. 3 is the decomposed schematic diagram of multi-functional nucleic acid detection apparatus shown in Fig. 1；
Fig. 4 is the structure diagram of base in Fig. 3；
Fig. 5 is the structure diagram of shell in Fig. 3；
Fig. 6 is the assembling schematic diagram of the other structures in addition to decapsidate in Fig. 3；
Fig. 7 is the structure diagram of transmission mechanism in Fig. 6；
Fig. 8 is the structure diagram of liquid sucting mechanism in Fig. 6；
Fig. 9 is the structure diagram of aspirating mechanism in Fig. 6.
Embodiment
For the ease of understanding the present invention, the present invention is described more fully below with reference to relevant drawings.In attached drawing
Give presently preferred embodiments of the present invention.But the present invention can realize in many different forms, however it is not limited to this paper institutes
The embodiment of description.On the contrary, the purpose for providing these embodiments is to make the understanding to the disclosure more thorough
Comprehensively.
It should be noted that when element is referred to as " being fixed on " another element, it can be directly on another element
Or there may also be element placed in the middle.When an element is considered as " connection " another element, it can be directly connected to
To another element or it may be simultaneously present centering elements.
Unless otherwise defined, all of technologies and scientific terms used here by the article is with belonging to technical field of the invention
The normally understood implication of technical staff is identical.Term used in the description of the invention herein is intended merely to description tool
The purpose of the embodiment of body, it is not intended that in the limitation present invention.
Incorporated by reference to Fig. 1, Fig. 2 and Fig. 3, the multi-functional nucleic acid detection apparatus 10 of an embodiment includes base 100, shell
200th, transmission mechanism 300, liquid sucting mechanism 400 and aspirating mechanism 500.Base 100 is tightly connected with shell 200, the two is engaged
Form airtight space.
Incorporated by reference to Fig. 3, Fig. 4 and Fig. 5, in the present embodiment, base 100 is equipped with multiple liquid baths, and the opening of liquid bath
End is in the airtight space.Multiple liquid baths can be used for a variety of different functions, reservoir can be such as used as, to store nucleic acid
Reagent (such as lysate and eluent etc.) needed for extraction, the reagent needed for PCR amplification (such as primer kit, PCR buffer solutions, enzyme,
DNTPs etc.) or detection needed for reagent (such as probe, enzyme, fluorometric reagent) etc.；Extraction tank can be and for example used as, for carrying
Take or elute sample of nucleic acid；Amplification groove can be and for example used as, for PCR amplification；Detect tank can be and for example used as, for containing
The reaction solution of dress for detection etc.；Waste liquid tank can be and for example used as, to contain waste liquid in reaction process etc..The number of all kinds of liquid baths
It can also be multiple that amount, which can be one, and such as in a specific embodiment, the liquid bath includes reservoir 101, extraction tank
102nd, groove 103, detect tank 104 and waste liquid tank 105 are expanded；And for example in another specific embodiment, the liquid bath only includes
Expand groove 103 and detect tank 104.
Base 100 is substantially in the form of annular discs.Each liquid bath may be designed to different size and dimensions, while arrange it as needed
Position, such as in one embodiment, reservoir 101 can be designed to different shape and depth according to actual needs.One
In a specific embodiment, multiple liquid baths are set around the edge of base 100, can such as be formed a circle in arcuation.Further,
When storage has reagent in liquid bath, the notch of liquid bath is sealed with the diaphragm seal that can be punctured.Diaphragm seal can be but not limited to aluminium foil
Paper etc., in order to the storage of reagent and the transport of whole device.
In one embodiment, liquid bath protrudes from the bottom of base 100 backwards to the bottom of shell 200, for insertion into outer
Connect in equipment.Correspondingly, the base unit of external equipment has multiple groove positions, for being inserted into for different liquid baths.It is external to set
Standby base unit can set heating module, the supplementary module of detection or magnet module in each groove position of correspondence (as being used for magnetic
Power stirs) etc., to coordinate each function liquid bath to perform corresponding function.
Incorporated by reference to Fig. 3, Fig. 4 and Fig. 5, shell 200 is substantially in hood-like structure.Match somebody with somebody with the sealing of base 100 200 bottom of shell
Close, the mode that can be such as connected through a screw thread is connected, can also connected by way of clamping.Between shell 200 and base 100
Can be through but not limited to sealing structures such as sealing rings.The top of shell 200 has multiple operative positions, such as transmission position 201 and pumping
Position 202 etc. is inhaled, wherein, transmission position 201 corresponds to transmission mechanism 300, and suction position 202 corresponds to aspirating mechanism 500.
In one embodiment, well 203 is additionally provided with shell 200.Well 203 corresponds at least one liquid of lower section
Groove, for adding sample to be tested into the liquid bath, such as in a specific embodiment, well 203 can be located at extraction tank
102 surface or positioned at amplification groove 103 surface, to add the sample of nucleic acid to be extracted or extracted sample of nucleic acid
Deng.Further, it is correspondingly provided with port lid 204 at the well 203.Port lid 204 is used to seal well 203, outer to ensure to make
The space-closed that shell 200 is formed with base 100.
Further, in a specific embodiment, which further includes sealing plate 205.Outside
Shell 200 is equipped with sliding slot 206 in the position close to the well 203, and sealing plate 205 is nested on sliding slot 206 and can be along cunning
Groove 206 is unidirectionally moved to well 203, and port lid 204 is pressed on well 203, prevent operation when port lid 204 unclamp or
Come off, be further ensured that the air-tightness of inner space.The unidirectional mobile sealing performance that may further ensure that sealing plate 205, with
Prevent maloperation or sealing plate 205 departs from the situation generation of sealing station caused by the extraneous factors such as vibration.
As shown in Figure 6 and Figure 7, transmission mechanism 300 is located at the top of shell 200, and shell 200 is stretched out to external in one end
Rotating driving device.Specifically, in one embodiment, one end of transmission mechanism 300 corresponds to the transmission position 201 on shell 200
Set.
In diagrammatically shown specific embodiment, transmission mechanism 300 is jacket type structure, including transmission axis 310, axis
Set 320, central axis connector 330 and axle sleeve rotation connection first 340.Transmission axis 310 is screw structure, bottom bracket axle 320
It is sleeved on transmission axis 310 and there is gap between transmission axis 310.Bottom bracket axle 320 is equipped with the axial direction along transmission axis 310
The limited mouth 322 of extension.It is driven axis 310 and independent rotation, specifically, axis is driven by independent connector with bottom bracket axle 320
Rotation connection first 330 with transmission axis 310 be fixedly connected, central axis connector 330 for be connected with rotating driving device with
Transmission axis 310 is driven to rotate；Axle sleeve rotation connection first 340 is fixedly connected with bottom bracket axle 320, and axle sleeve rotation connection first 340 is used
In being connected with rotating driving device to drive bottom bracket axle 320 to rotate.
Incorporated by reference to Fig. 6, Fig. 7 and Fig. 8, liquid sucting mechanism 400 is connected with transmission mechanism 300, and liquid sucting mechanism 400 is located at the gas
In close space, and rotation can be driven to be switched between different liquid baths by transmission mechanism 300, or in the vertical direction
Move up and down.
Specifically, in one embodiment, one end of liquid sucting mechanism 400 is sleeved on transmission axis 310 through limited mouth 322
And it is sequentially connected and (is threadedly coupled) with transmission axis 310.When being driven the rotation of axis 310, it can drive and be arranged on it
Liquid sucting mechanism 400 rises or falls；When bottom bracket axle 320 rotates, since the inner wall of bottom bracket axle 320 can be with liquid sucting mechanism 400
Abut against, so can synchronous drive liquid sucting mechanism 400 rotate.
More specifically, in diagrammatically shown embodiment, liquid sucting mechanism 400 includes linking arm 410, imbibition cavity 420 and inhales
Liquid tip 430.One end of linking arm 410 is sequentially connected through limited mouth 322 and transmission axis 310.Imbibition cavity 420 is positioned at company
The other end of arm 410 is connect, imbibition cavity 420 is connected with imbibition tip 430.
Incorporated by reference to Fig. 6, Fig. 8 and Fig. 9, aspirating mechanism 500 is connected with imbibition cavity 420, with by aspirating mechanism 500 drive from
Liquid bath draws liquid or discharges liquid to liquid bath.
Specifically, in one embodiment, aspirating mechanism 500 has multiple pulsometers 510.Pulsometer 510 is plunger type
Pump body structure, by aspirate or push action can realize imbibition or discharge opeing.The volume difference of multiple pulsometers 510 is to form
Multistage suction system, to draw or exclude different amounts of liquid, ensures precision when absorption or discharge opeing.
Further, in diagrammatically shown embodiment, imbibition cavity 420 includes imbibition groove 421 and capping 422.Capping 422 is close
Capping is located on imbibition groove 421.Capping 422 is equipped with imbibition connecting pole 423, and imbibition connecting pole 423 connects with imbibition cavity 420
It is logical.Correspondingly, aspirating mechanism 500 is equipped with suction connecting pole 520.Multiple pulsometers 510 are located on the suction connecting pole 520 and each
Connected from suction connecting pole 520.By both ends respectively with aspirating connecting pole 520 between aspirating mechanism 500 and liquid sucting mechanism 400
And the hose (not shown) connection that imbibition connecting pole 423 connects.Hose has certain length, to ensure that liquid sucting mechanism 400 exists
With certain freedom of movement when rotating or lifting.
It please join Fig. 3 and Fig. 4, in a specific embodiment, which, which further includes, is located at gas
Test-strips 600 in close space.Test-strips 600 are close to the liquid bath of detection for absorbing the liquid in the liquid bath.To inspection
After liquid being added in the liquid bath of survey, with the generation of chromatography phenomenon, component and 600 corresponding site of test-strips in amplified production
Component mutually hybridizes, and then forms coloured band.The band can be detected by the detection module in external equipment, and then be formed
Sentence read result.It is understood that in other embodiments, which can also use other detection modes,
Fluoroscopic examination is such as carried out, correspondingly, the bottom of the corresponding detect tank 104 of base 100 is arranged to transparent.
In diagrammatically shown specific embodiment, when in use, user can be first for the multi-functional nucleic acid detection apparatus 10
Port lid 204 is opened, adds the sample or pre- of certain volume into the extraction tank 102 for be stored with sample lysate using sample needle
Sample is handled, is then covered port lid 204 on well 203, and sealing plate 205 is slided on well 203.This is multi-functional
Nucleic acid detection apparatus 10 is put into the base unit of external equipment.After multi-functional nucleic acid detection apparatus 10 is installed, transmission mechanism
300th, aspirating mechanism 500 etc. matches machinery corresponding with external equipment, and adding for nucleic acid amplification is useful in base unit
The structures such as thermal modules, the supplementary module of detection and transportable magnet.Now by taking the sample extracted as an example, to more work(
Specifically amplification and detecting step are illustrated 10 internal operation of energy nucleic acid detection apparatus, specific as follows：
1) imbibition tip 430 is gone to the surface of extraction tank 102 by rotating middle shaft set 320, and by base unit
Alignment sensor verifies the movement；
2) air in the push rod discharge imbibition tip 430 of pulsometer 510 is depressed, while rotates transmission axis 310, is passed through
Screw structure drives imbibition tip 430 to be moved downwardly into extraction tank 102 and makes the tip of imbibition tip 430 to extraction tank 102
Liquid level below；
3) push rod is suitably unclamped, the recovery spring rise in pulsometer 510, makes sample solution flow into imbibition tip 430；
4) transmission axis 310 is rotated, drives imbibition tip 430 to move upwardly off extraction tank 102；
5) imbibition tip 430 is gone to amplification groove 103 by rotating middle shaft set 320, using the alignment sensor in base unit
Verify the movement；
6) transmission axis 310 is rotated, drives imbibition tip 430 to move down the sealing at the notch for puncturing amplification groove 103
Film, into filling the amplification groove 103 of amplification system, and makes below tip to the liquid level of imbibition tip 430；
7) liquid in push rod discharge imbibition tip 430 is depressed；
8) transmission axis 310 is rotated, drives imbibition tip 430 to move upwardly off amplification groove 103；
9) rotating middle shaft set 320 rotates imbibition tip 430 to the upper position for the reservoir 101 for storing the mineral oil such as glycerine
Put, which is verified using the alignment sensor of base unit；
10) transmission axis 310 is rotated, drives imbibition tip 430 to move down the sealing at the notch for puncturing reservoir 101
Film, into and make below tip to the liquid level of imbibition tip 430；
11) push rod is suitably unclamped, recovering spring rise makes mineral oil flow into imbibition tip 430；
12) transmission axis 310 is rotated, drives imbibition tip 430 to move upwardly off reservoir 101；
13) rotating middle shaft set 320 rotates imbibition tip 430 to the top position of amplification groove 103, using determining for base unit
Level sensor verifies the movement；
14) transmission axis 310 is rotated, drives imbibition tip 430 to be moved downwardly into amplification groove 103 and makes imbibition tip
Below 430 tip to liquid level.；
15) liquid of push rod discharge imbibition tip 430 is depressed；
16) transmission axis 310 is rotated, drives imbibition tip 430 to move upwardly off amplification groove 103.
17) base unit carries out the program of nucleic acid amplification；
18) it is room temperature that base unit, which keeps the temperature of amplification groove 103,；
19) transmission axis 310 is rotated, drives imbibition tip 430 to be moved downwardly into amplification groove 103 and makes tip to liquid level
Below；
20) push rod is suitably unclamped, recovering spring rise makes amplified production flow into imbibition tip 430；
21) transmission axis 310 is rotated, drives imbibition tip 430 to move upwardly off amplification groove 103；
22) rotating middle shaft set 320 rotates imbibition tip 430 to the top position of detect tank 104, using determining for base unit
Level sensor verifies the movement；
23) transmission axis 310 is rotated, drives imbibition tip 430 to move down the sealing at the notch for puncturing detect tank 104
Film, into and make below tip to liquid level；
24) liquid of push rod discharge imbibition tip 430 is depressed；
25) transmission axis 310 is rotated, drives imbibition tip 430 to move upwardly off detect tank 104；
26) test-strips 600 of the detect tank 104 equipped with detection amplified production, after the liquid addition, with the hair of chromatography phenomenon
Raw, component and the component of test-strips corresponding site mutually hybridize in amplified production, and then form coloured band, which can
Captured by base unit detection module, form sentence read result.
Some above-mentioned steps can be carried out more than once.In addition, it is appreciated that in other embodiments, be not limited to according to upper
State step execution.
Above-mentioned multi-functional nucleic acid detection apparatus 10 can be contained each by setting multiple liquid baths on base 100 in liquid bath
Plant reagent needed for reaction or as container of reaction or detection etc., thus multiple functions can be gathered in one, can such as use
In the extraction, amplification and detection of nucleic acid, or the amplification and detection etc. of at least one heavy target template extracted.The multifunctional core
Sour detection device 10 is coordinated by base 100 and the sealing of shell 200, is formed an independent cavity, is taken by manipulation device
Transmission mechanism 300, liquid sucting mechanism 400 and the aspirating mechanism 500 of band can realize that liquid sucting mechanism 400 is cut between different liquid baths
Change, and can realize imbibition or discharge opeing in different liquid baths, it is simple to operate.
The transmission axis 310 and bottom bracket axle 320 and imbibition machine of the transmission mechanism 300 of the multi-functional nucleic acid detection apparatus 10
Structure 400 is all located in airtight space, when liquid sucting mechanism 400 is rotated with upper and lower movement, since airtight space volume does not have substantially
Change, therefore, will not cause the change of air pressure in airtight space, thereby may be ensured that the stability of air pressure in airtight space, into
And can ensure precision of the liquid sucting mechanism 400 in imbibition or discharge opeing from effects of air pressure, be conducive to improve the standard of testing result
True property and reliability.And imbibition is driven by the rotation of transmission axis 310 and bottom bracket axle 320 inside airtight space respectively
400 upper and lower movement of mechanism and rotation, when device assembles, can be saved in transmission mechanism 300 and 200 contact site of shell needs
The trouble effectively sealed using sealing structures such as many sealing rings with being formed, structure design is simpler, and external rotation driving fills
Put driving effect it is more preferable, the precision of rotary motion is easier control.
The multi-functional nucleic acid detection apparatus 10 is mainly disposable product, and as disposable consumptive material, it is tied
Structure is simple, it can be achieved that small size, and multiple functions can be gathered in detection of nucleic acids in one, easy to operate, can examine nucleic acid
Survey and used in the medical environment for more lacking professional technician and experiment condition, be conducive to the application and popularization of detection of nucleic acids.
Each technical characteristic of embodiment described above can be combined arbitrarily, to make description succinct, not to above-mentioned reality
Apply all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, the scope that this specification is recorded all is considered to be.
Embodiment described above only expresses the several embodiments of the present invention, its description is more specific and detailed, but simultaneously
Cannot therefore it be construed as limiting the scope of the patent.It should be pointed out that come for those of ordinary skill in the art
Say, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection of the present invention
Scope.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (10)
- A kind of 1. multi-functional nucleic acid detection apparatus, it is characterised in that including base, transmission mechanism, liquid sucting mechanism, aspirating mechanism and The shell being tightly connected with the base；Wherein, the base is equipped with multiple liquid baths, and the base is engaged to form airtight space with the shell, the liquid bath Openend be located in the airtight space；The transmission mechanism is located on the top of the shell, and the shell is stretched out to external rotating driving device in one end, The transmission mechanism includes transmission axis and bottom bracket axle, and the bottom bracket axle is sleeved on the transmission axis, the transmission axis with The bottom bracket axle can be driven by the rotating driving device, and can independently be rotated, it is described transmission axis and it is described in Axle sleeve is respectively positioned in the airtight space；The liquid sucting mechanism is located in the airtight space, and the liquid sucting mechanism is connected with the transmission mechanism, in the transmission Axis rotates and the bottom bracket axle rotates and is respectively used for driving the liquid sucting mechanism and moved up and down and rotary motion in vertical direction To be switched between different liquid baths；The aspirating mechanism is connected with the liquid sucting mechanism, for make the liquid sucting mechanism from the liquid bath draw liquid or to The liquid bath discharges liquid.
- 2. multi-functional nucleic acid detection apparatus as claimed in claim 1, it is characterised in that the liquid bath is backwards to the bottom of the shell Portion protrudes from the bottom of the base, for insertion into external equipment.
- 3. multi-functional nucleic acid detection apparatus as claimed in claim 1, it is characterised in that the liquid bath include at least amplification groove and Detect tank, multiple liquid baths are set around the transmission mechanism.
- 4. multi-functional nucleic acid detection apparatus as claimed in claim 3, it is characterised in that further include and be located in the airtight space Test-strips, the test-strips are close to the detect tank for absorbing the liquid in the detect tank.
- 5. multi-functional nucleic acid detection apparatus as claimed in claim 1, it is characterised in that the transmission mechanism further includes axis and turns Dynamic connector and axle sleeve rotation connection head；The transmission axis be screw structure, the bottom bracket axle with it is described be driven axis it Between there is gap, the bottom bracket axle is equipped with the axially extending limited mouth along the transmission axis；The central axis connector It is fixedly connected with the transmission axis, the central axis connector is used to be connected with rotating driving device to drive the transmission Central axis；The axle sleeve rotation connection head is fixedly connected with the bottom bracket axle, and the axle sleeve rotation connection head is used for and rotation Driving device is connected to drive the bottom bracket axle to rotate；One end of the liquid sucting mechanism is sleeved on the transmission axis through the limited mouth and connects with the transmission middle axle driven Connect；When the transmission central axis, the liquid sucting mechanism can be driven to rise or fall；When the bottom bracket axle rotates, institute Stating the inner wall of bottom bracket axle can abut against with the liquid sucting mechanism to drive the liquid sucting mechanism to rotate.
- 6. multi-functional nucleic acid detection apparatus as claimed in claim 5, it is characterised in that the liquid sucting mechanism include linking arm, Imbibition cavity and imbibition tip；One end of the linking arm is connected through the limited mouth with the transmission middle axle driven, described Imbibition chamber body is located at the other end of the linking arm, and the imbibition cavity is connected with the imbibition tip, and the imbibition cavity is also Connected with the aspirating mechanism.
- 7. multi-functional nucleic acid detection apparatus as claimed in claim 6, it is characterised in that the imbibition cavity include imbibition groove and Capping, the capping sealing cover are located on the imbibition groove, and the capping is equipped with imbibition connecting pole, and the aspirating mechanism is equipped with Connecting pole is aspirated, connecting pole and the suction are aspirated with described respectively by both ends between the aspirating mechanism and the liquid sucting mechanism The hose connection of liquid connecting pole connection.
- 8. such as multi-functional nucleic acid detection apparatus according to any one of claims 1 to 7, it is characterised in that the aspirating mechanism With multiple pulsometers, the volume differences of multiple pulsometers is to form multistage suction system.
- 9. such as multi-functional nucleic acid detection apparatus according to any one of claims 1 to 7, it is characterised in that the shell is equipped with Well, the well correspond at least one liquid bath, and the well is equipped with port lid.
- 10. multi-functional nucleic acid detection apparatus as claimed in claim 9, it is characterised in that further include sealing plate, the shell exists Sliding slot is equipped with close to the position of the well, and the sealing plate is nested on the sliding slot and can be along the sliding slot to described Well unidirectionally moves.
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| CN201711461882.1A CN107964505A (en) | 2017-12-28 | 2017-12-28 | Multi-functional nucleic acid detection apparatus |
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| CN201711461882.1A CN107964505A (en) | 2017-12-28 | 2017-12-28 | Multi-functional nucleic acid detection apparatus |
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Inventor after: Wang Jianping Inventor before: Zou Bingjie Inventor before: Wang Jianping |