CN111808731A - A nucleic acid amplification tube and a fully enclosed nucleic acid detection system - Google Patents

Abstract

The present invention provides a nucleic acid amplification tube and a fully enclosed nucleic acid detection system. The nucleic acid amplification tube is provided with a temperature display area. With this technical solution, when you are in some poor or backward areas and cannot obtain constant temperature equipment for nucleic acid amplification reaction in time, you can directly place the nucleic acid amplification tube in the heat preservation container, add hot water and cold water, and display the temperature through the temperature display. The temperature displayed in the area, adjust the amount of hot or cold water, and adjust to the appropriate reaction temperature, without the need for additional constant temperature water bath equipment, the nucleic acid amplification reaction can be carried out on the spot for instant detection.

Description

A nucleic acid amplification tube and a fully enclosed nucleic acid detection system

technical field

The invention relates to the field of detection, in particular to a nucleic acid amplification tube and a fully enclosed nucleic acid detection system.

Background technique

Nucleic acid diagnosis is one of the most dynamic segments in the future IVD (in vitro diagnostic) industry. The increase in the prevention and control of infectious diseases in my country, the promotion of nucleic acid detection by blood screening, and the development of individualized medicine are the main driving forces for the development of domestic nucleic acid diagnosis. Driven by these factors, the future growth rate of domestic nucleic acid diagnosis will be between 25-30%, significantly exceeding the average growth rate of the domestic IVD industry. On the one hand, nucleic acid diagnosis benefits large medical centers and realizes early, rapid, specific, and high-throughput detection of pathogens and genetic diseases.

POCT (point-of-care testing), Chinese for "point-of-care testing", is an emerging sub-industry of in vitro diagnosis (IVD). , a new class of methods to get test results quickly. The main criteria of POCT are that no fixed testing site is required, reagents and instruments are portable, and can be operated in a timely manner. POCT undertakes the functions of a laboratory but does not require traditional hospital laboratory equipment, and can serve patients 24 hours a day regardless of time and location.

Especially for some poor or backward areas, due to poverty, poor sanitary conditions, low hygiene awareness, malnutrition and other reasons, they have always been the hardest hit areas by infectious diseases. The incidence of infectious diseases is high, the mortality rate is high, and the high cost of treatment is unaffordable for ordinary families. However, in these areas, advanced infectious disease testing methods cannot be popularized, mainly due to the difficulty of power supply in most areas and the inability to operate large-scale instruments; the cost of large-scale medical equipment and the corresponding maintenance equipment costs cannot be afforded; site restrictions; patients cannot afford the high cost inspection fees, etc. The amplification reaction in the nucleic acid amplification tube needs to be carried out in a specific temperature range, and it needs to be carried out in a specific closed laboratory to avoid sample leakage and pollution of the environment. The closed laboratory cannot detect on the spot, which limits the immediacy of nucleic acid detection.

Therefore, it is an urgent problem to be solved in the art to provide a method that can perform nucleic acid detection in real time.

SUMMARY OF THE INVENTION

The purpose of the present invention is to solve the problem that nucleic acid detection cannot be performed on the spot in the prior art. In order to solve the above problems, the present invention discloses a nucleic acid amplification tube with a new structure and a fully enclosed nucleic acid detection system, through which nucleic acid amplification tube and detection system can be used for nucleic acid detection at any time, which greatly promotes nucleic acid detection in poverty or backwardness. Regional application.

The invention discloses a nucleic acid amplification tube, comprising a nucleic acid amplification tube body and a nucleic acid amplification tube cover. The nucleic acid amplification tube body is sealed at the lower end and opened at the upper end. The nucleic acid amplification tube cover seals the opening of the nucleic acid amplification tube body. A closed nucleic acid solution storage cavity is formed, and a temperature display area is arranged on the nucleic acid amplification tube.

With this technical solution, when you are in some poor or backward areas and cannot obtain constant temperature equipment for nucleic acid amplification reaction in time, you can directly place the nucleic acid amplification tube in the container, add hot and cold water, and the temperature display area will display The current temperature of the nucleic acid amplification tube, through the temperature displayed in the temperature display area, adjust the amount of hot or cold water to the appropriate nucleic acid amplification reaction temperature, without the need for additional constant temperature water bath equipment, the nucleic acid solution in the nucleic acid amplification tube can be carried out on the spot The nucleic acid amplification reaction can be detected immediately after the reaction is completed.

According to another specific embodiment of the present invention, the temperature display area includes a reversible temperature test paper.

According to another specific embodiment of the present invention, the temperature display area includes a first temperature display area and a second temperature display area, and the first temperature display area and the second temperature display area are respectively coated with reversible thermochromic materials with different color changing temperatures .

According to another specific embodiment of the present invention, the temperature display area is arranged on the tube cover, or the temperature display area is arranged on the side wall of the tube.

The invention also discloses a fully enclosed nucleic acid detection system, comprising a casing and any of the aforementioned nucleic acid amplification tubes, a cavity is arranged in the casing, and a nucleic acid solution adding area is arranged on the upper surface of the casing,

The cavity below the nucleic acid solution addition area is provided with a chromatography test paper and a puncturing mechanism in sequence from bottom to top, and the nucleic acid solution addition area is set for the insertion of the nucleic acid amplification tube, and the nucleic acid solution is inserted into the nucleic acid amplification tube to add After the area, the cavity is sealed, the piercing mechanism pierces the nucleic acid amplification tube, and the nucleic acid solution flows into the cavity.

According to another specific embodiment of the present invention, a destruction liquid storage part is further provided in the cavity, and the destruction liquid storage part can be opened to allow the destruction liquid to flow into the cavity.

Description of drawings

Below in conjunction with the accompanying drawings and specific embodiments, the present invention will be described in further detail:

1 is a cross-sectional view of a nucleic acid amplification tube provided by the present invention;

2 is a schematic structural diagram of a nucleic acid amplification tube provided by the present invention;

3 is a cross-sectional view of the nucleic acid amplification tube provided by the present invention and the detection system in a matched state;

4 is a cross-sectional view of the nucleic acid amplification tube and the detection system provided by the present invention in another state of cooperation;

5 is a schematic structural diagram of the nucleic acid amplification tube provided by the present invention and the detection system in a coordinated state;

6 is a schematic diagram of an explosion of a nucleic acid amplification tube and a detection system provided by the present invention;

Fig. 7 is the structural representation of the puncturing mechanism provided by the present invention;

FIG. 8 is a schematic structural diagram of the puncturing mechanism provided by the present invention.

Reference number:

Nucleic acid amplification tubes 100

side wall 110

first protrusion 111

Down Tube Wall 120

Recess 121

Tube cap 130

Nucleic acid solution storage chamber 140

seal ring 150

Temperature display area 160

Shell 200

cavity 210

Destruction Fluid Storage 220

Liquid Seals 230

Chromatography test paper 240

Piercing Mechanism 250

Positioning Section 251

Fourth limit part 2511

The third protrusion 2512

Pierced 252

fluid channel 253

Nucleic acid solution addition area 260

first groove 261

The third limit part 262

Test result observation area 270

Elastic press down structure 280

Detailed ways

The embodiments of the present invention are described below by specific embodiments, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. Although the description of the invention will be presented in conjunction with the preferred embodiment, this does not mean that the features of the invention are limited to this embodiment. On the contrary, the purpose of introducing the invention in conjunction with the embodiments is to cover other options or modifications that may be extended based on the claims of the invention. The following description will contain numerous specific details in order to provide a thorough understanding of the present invention. The invention may also be practiced without these details. Furthermore, some specific details will be omitted from the description in order to avoid obscuring or obscuring the gist of the present invention. It should be noted that the embodiments of the present invention and the features of the embodiments may be combined with each other under the condition of no conflict.

In the description of this embodiment, it should be noted that the orientations or positional relationships indicated by the terms "upper", "lower", "inside", "bottom", etc. are based on the orientations or positional relationships shown in the drawings, or are The orientation or positional relationship that the product of the invention is usually placed in use is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, Therefore, it should not be construed as a limitation of the present invention.

The terms "first", "second", "third", etc. are only used to differentiate the description and should not be construed as indicating or implying relative importance.

In the description of this embodiment, it should also be noted that, unless otherwise expressly specified and limited, the terms "arranged", "connected" and "connected" should be understood in a broad sense, for example, it may be a fixed connection or a Removable connection, or integral connection; can be mechanical connection, can also be electrical connection; can be directly connected, can also be indirectly connected through an intermediate medium, can be internal communication between two components. For those of ordinary skill in the art, the specific meanings of the above terms in this embodiment can be understood in specific situations.

In order to make the objectives, technical solutions and advantages of the present invention clearer, the embodiments of the present invention will be further described in detail below with reference to the accompanying drawings.

As shown in FIG. 1 and FIG. 2, the present invention discloses a nucleic acid amplification tube 100, which includes a nucleic acid amplification tube body and a nucleic acid amplification tube cover 130. The lower end of the nucleic acid amplification tube body is sealed and the upper end is open for nucleic acid amplification. The tube body includes a side tube wall 110 and a lower tube wall 120. The nucleic acid amplification tube cover 130 seals the opening of the nucleic acid amplification tube body to form a closed nucleic acid solution storage cavity 140, and the nucleic acid amplification tube body is also provided with a temperature display area. 160.

With this technical solution, when you are in some poor or backward areas and cannot obtain constant temperature equipment for nucleic acid amplification reaction in time, you can directly place the nucleic acid amplification tube in the heat preservation container, add hot water and cold water, and display the temperature through the temperature display. The temperature displayed in the area 160 can be adjusted to a suitable reaction temperature by adjusting the amount of hot or cold water, and the nucleic acid amplification reaction can be performed on the spot without additional constant temperature water bath equipment.

According to another specific embodiment of the present invention, the nucleic acid amplification tube 100 in the present invention is a specially-made nucleic acid amplification tube, and the side wall 110 of the nucleic acid amplification tube 100 can be composed of a series of materials, which preferably have good thermal conductivity, Made of high strength and good fluidity materials, such as metals, alloys, thermally conductive plastics and organic composite materials, the height is 1-3cm, preferably 2cm, and the approximate shape can be similar to ordinary nucleic acid amplification tubes, but there are differences.

According to another specific embodiment of the present invention, the temperature display area 160 may be a reversible temperature test paper. A suitable reversible temperature test paper can be selected according to the temperature of the nucleic acid amplification reaction. For example, when the nucleic acid amplification reaction temperature is 38 degrees, you can choose THERMAX reversible temperature test paper of 0 to 50 °C; if the nucleic acid amplification reaction requires 63 degrees, you can choose THERMAX reversible temperature test paper of 50 to 100 °C . In addition, special temperature test strips can also be customized to suppliers according to the actual situation.

According to another specific embodiment of the present invention, the temperature display area 160 includes a first temperature display area and a second temperature display area, and the first temperature display area and the second temperature display area are respectively coated with reversible temperature-sensitive discoloration with different discoloration temperatures Material. The discoloration temperature of the thermochromic material can be set according to actual needs, and the specific reversible thermochromic material can be a commercially available product. For a certain amplification reaction, if the reaction temperature needs to be between the first temperature T1 and the second temperature T2, two kinds of temperature-sensitive color-changing materials can be coated on the surface of the nucleic acid amplification tube 100, and the first temperature-sensitive color-changing material can be selected. The discoloration temperature of the material is the first temperature T1, and the discoloration temperature of the second thermochromic material is the second temperature T2. In this way, when the first temperature-sensitive material changes color while the second temperature-sensitive material does not change color during the amplification reaction, it indicates that the temperature is suitable for the amplification reaction in the nucleic acid amplification tube 100 to be performed. In this way, the nucleic acid amplification tube 100 can be directly put into the thermos, and the amount of hot and cold water can be adjusted to control the temperature of the water in the thermos, so that the amplification reaction can be maintained. No constant temperature equipment is required, and the samples can be analyzed anytime, anywhere. detection.

For example, if the optimal reaction temperature is around 38 degrees, two temperature-sensitive coatings can be used, which are greater than 38 and less than 38 degrees, preferably 37 and 39 degrees. If the optimal reaction temperature is 63 degrees, then the temperature The temperature-sensitive paint can be selected as 62 and 64, and the shape of the temperature-sensitive paint can be arbitrary, but it is preferably an Arabic numeral corresponding to the temperature, such as a temperature-sensitive material with a color change of 38 degrees, which is displayed as "38". In this way, the temperature of the nucleic acid amplification tube 100 can be more directly reflected.

Further, if the nucleic acid amplification tube 100 is used in various amplification reaction systems with different temperatures, various temperature-sensitive color-changing materials can be provided.

According to another specific embodiment of the present invention, in order to facilitate the nucleic acid amplification tube 100 to be pierced by the piercing mechanism 250 after being inserted into the fully enclosed nucleic acid detection system, a cut can be provided on the lower tube wall 120 of the nucleic acid amplification tube 100 , when the piercing mechanism 250 pierces the lower tube wall 120 of the nucleic acid amplification tube 100, the lower tube wall 120 of the nucleic acid amplification tube 100 is broken along the position of the incision; The lower tube wall 120 is set thinner, so that the piercing mechanism 250 can easily pierce the nucleic acid amplification tube 100 .

Further, since the lower tube wall 120 of the nucleic acid amplification tube 100 is provided with incisions or the lower tube wall 120 is set to be thinner, the nucleic acid amplification tube 100 is not inserted into the fully enclosed nucleic acid detection system before being inserted into the fully enclosed nucleic acid detection system. May break along cuts or thin spots. Therefore, according to another specific embodiment of the present invention, the lower tube wall 120 of the nucleic acid amplification tube 100 is provided with a concave portion 121 recessed toward the tube cover 130, and the incision is provided in the concave portion 121; or only the concave portion 121 is provided. The inner nucleic acid amplification tube 100 has a relatively thin tube wall, preferably at least the central position of the lower tube wall 120 of the nucleic acid amplification tube 100 is thinner, which can effectively prevent the nucleic acid amplification tube 100 from being damaged during the picking and placing process.

According to another specific embodiment of the present invention, the general nucleic acid amplification tube 100 has an open-cap structure, that is, the tube cap 130 is a cap that can be opened and closed, and the sample that needs to be amplified and the reagents related to the amplification reaction system After placing the nucleic acid amplification tube 100, the lid is closed to achieve sealing. In addition, the upper part of the nucleic acid amplification tube 100 can also be directly a closed structure, that is, the tube cover 130 and the side tube wall 110 are fixedly connected, or even directly integrally formed and cannot be opened. When in use, a syringe with a fine needle pierces the cap 130 of the nucleic acid amplification tube 100, injects the reaction system, and then seals the breach with a parafilm or a wax drop with a higher melting point, so that the nucleic acid amplification tube 100 can be better realized. seal.

According to another specific embodiment of the present invention, from the viewpoint of easy observation, the temperature display area 160 is arranged on the tube cover, or the temperature display area 160 is arranged on the side wall of the tube.

According to another specific embodiment of the present invention, one or more first protrusions 111 are provided on the outer surface of the side pipe wall 110 , if there are multiple first protrusions 111 , the multiple first protrusions 111 may be located at On the same cross section of the side pipe wall 110, at least part of the side pipe wall 110 is a cylindrical side pipe wall, the outer surface of the cylindrical side pipe wall is provided with an external thread (not shown in the figure), and the external thread is located on the first convex surface. below the section 111.

The structure of the first convex portion 111 is not particularly limited, as long as it can fit the first groove 261 to limit the position of the nucleic acid amplification tube 100 relative to the cylindrical channel in the axial direction, and the first convex portion 111 can It is a convex point, it can also be a convex strip extending in the circumferential direction, or a complete circular convex ring. From the perspective of more effective and more stable restriction of the position along the axial direction of the nucleic acid amplification tube 100 , preferably the first convex portion 111 is a complete circular convex ring, or a plurality of them are uniform along the circumferential direction of the side tube wall 110 . Distributed bumps or ridges.

According to another specific embodiment of the present invention, as shown in FIG. 1 and FIG. 2 , the first convex portion 111 on the side wall 110 of the nucleic acid amplification tube 100 is a circular convex ring, and the nucleic acid amplification portion under the first convex portion 111 is a circular convex ring. The side pipe wall 110 of the extension pipe 100 is a cylindrical side pipe wall, and the cylindrical side pipe wall is provided with an external thread matched with the internal thread of the positioning portion 251 of the puncturing mechanism 250 .

Further, as shown in FIG. 3 to FIG. 6 , a fully enclosed nucleic acid detection system is also disclosed in the present invention, which includes a housing 200 and any of the aforementioned nucleic acid amplification tubes 100 . The housing 200 is provided with a cavity 210 and the housing The upper surface of 200 is provided with a nucleic acid solution adding area 260,

The chromatographic test paper 240 and the puncturing mechanism 250 are arranged in the cavity below the nucleic acid solution adding area 260 in sequence from bottom to top. After the tube 100 is inserted into the nucleic acid solution adding area 260 , the cavity 210 is sealed, the piercing mechanism 250 pierces the lower tube wall 130 of the nucleic acid amplification tube 100 , and the nucleic acid solution flows into the cavity 210 .

In some poor or backward areas, using the nucleic acid amplification tube 100 provided by the present invention, the nucleic acid amplification reaction can be completed immediately, and then the nucleic acid amplification tube 100 can be inserted into the nucleic acid solution adding area 260 to quickly complete the nucleic acid amplification detection, and since the nucleic acid amplification tube 100 and the nucleic acid solution adding area 260 are sealed connection, it can effectively prevent the sample from volatilizing into the air and pollute the environment, and there is no need to transport the nucleic acid sample to be tested to a special closed laboratory for detection. , can be detected immediately.

According to another specific embodiment of the present invention, the cavity 210 is further provided with a destruction liquid storage part 220 , and the destruction liquid storage part 220 can be opened to allow the destruction liquid to flow into the cavity 210 . The nucleic acid destruction reagent is used to remove the residual nucleic acid in the fully enclosed nucleic acid detection system to further avoid possible contamination in the subsequent process.

According to another specific embodiment of the present invention, the casing 200 is provided with a cavity 210, and the cavity 210 is provided with a destruction liquid storage part 220, a chromatography test paper 240 and a puncturing mechanism 250 in sequence from bottom to top, and the destruction liquid storage part The upper surface of 220 is provided with an opening, and the opening is sealed by the liquid seal 230, the liquid seal 230 can be opened, and the destruction liquid storage part 220 can store nucleic acid destruction reagents, such as sodium hypochlorite solution or commercial DNA detergents.

The upper surface of the housing 200 is provided with a nucleic acid solution addition area 260 and a detection result observation area 270. The nucleic acid solution addition area 260 includes a cylindrical channel, the cylindrical channel includes an insertion end and a non-insertion end, and the insertion end is configured for nucleic acid amplification. The tube 100 is inserted, and the shape of the insertion end matches the nucleic acid amplification tube 100 to seal the cavity 210. The insertion end of the cylindrical channel is provided with an annular first groove 261. When the nucleic acid amplification tube 100 is inserted into the cylindrical channel , the first convex portion 111 and the first groove 261 cooperate with each other to limit the axial movement of the nucleic acid amplification tube 100 relative to the cylindrical channel,

As shown in FIG. 7 and FIG. 8 , the piercing mechanism 250 includes a cylindrical positioning portion 251 and a piercing portion 252 located on the central axis of the positioning portion 251. The piercing mechanism 250 is further provided with a fluid channel 253. The non-insertion end is provided with a third limiting portion 262 , and the positioning portion 251 is provided with a fourth limiting portion 2511 . The central axis is the rotation axis, the inner surface of the positioning portion 251 is provided with an inner thread (not shown in the figure), and the outer surface of the nucleic acid amplification tube 100 is provided with an outer thread matched with the inner thread.

When the nucleic acid amplification tube 100 is inserted into the cylindrical channel and the nucleic acid amplification tube 100 is rotated, as shown in FIG. 3 , since the first convex portion 111 fits with the annular first groove 261 , the nucleic acid amplification tube 100 can only rotate without being able to insert inward relative to the cylindrical channel. And as the nucleic acid amplification tube 100 rotates, since the outer thread on the side wall 110 of the nucleic acid amplification tube 100 is matched with the inner thread on the positioning part 251 , the positioning part 251 will drive the piercing part 252 to the nucleic acid amplification tube 100 . Move to the position shown in FIG. 4 (in FIG. 4 , the liquid seal 230 has been opened, but the liquid seal 230 cannot be opened before the detection is completed, and only the piercing mechanism 250 and the nucleic acid amplification tube 100 are shown here in FIG. 4 . relative positional relationship), the nucleic acid amplification tube 100 is punctured, and the nucleic acid solution flows into the cavity 210 from the fluid channel 253 . The chromatography test paper 240 in the cavity 210 can detect the sample, and the testing personnel can observe the testing result through the testing result observation area 270 .

By adopting the above technical solution, during the detection process, the cavity 210 is always kept sealed to prevent the amplification product from leaking to the outside. Further, after recording the detection results, as shown in FIG. 4 , the liquid seal 230 can be opened, and the chromatography test paper 240 can react with the nucleic acid destroying reagent to completely remove the residual nucleic acid in the fully enclosed nucleic acid detection system. In this way, even if the nucleic acid amplification tube 100 accidentally falls off in the subsequent process, or the fully enclosed nucleic acid detection system is damaged, resulting in internal exposure, it will not cause any pollution.

In addition, in the prior art in which the nucleic acid amplification tube 100 is inserted downward while the puncturing mechanism 250 remains stationary, since the gas in the casing 200 is compressed, amplification products may be amplified from the nucleic acid. There is leakage in the gap where the tube 100 and the casing 200 are connected, causing contamination. With the completely closed nucleic acid detection system provided by the present invention, the device allows the nucleic acid amplification tube 100 to remain stationary in the axial direction, but is rotated to move the piercing mechanism 250 upward to pierce the nucleic acid amplification During the puncturing process of the tube 100, the air pressure in the casing 200 remains unchanged, and the aerosol of the amplification product will not leak to the outside, which can better avoid contamination. In addition, the nucleic acid amplification tube 100 and the housing 200 are connected by threads, the connection structure is more stable, and it is not easy to fall off, which further avoids the exposure of the amplification product and the possibility of contamination. And finally, the nucleic acid destruction reagent is used to remove the residual nucleic acid in the fully enclosed nucleic acid detection system to further avoid possible contamination in the subsequent process.

According to another specific embodiment of the present invention, the third limiting portion 262 is a second groove extending in the axial direction, and the fourth limiting portion 2511 is a second convex portion. The second convex portion may be a convex point or a convex strip extending in the axial direction; and the length of the second groove should allow the piercing mechanism 250 to move upward to the position where the nucleic acid amplification tube 100 is pierced. Before the nucleic acid amplification tube 100 is inserted into the positioning portion 251 and starts to rotate, the second convex portion is located at the lowermost end of the second groove. Move upward along the second groove until the piercing mechanism 250 pierces the lower tube wall 120 of the nucleic acid amplification tube 100 .

Further, from a more reliable point of view, a plurality of second protrusions and a second groove extending along the axial direction can be provided. Preferably, a plurality of second protrusions and a matching groove along the axis The second extending grooves are evenly distributed along the circumference of the cylindrical channel.

It is easily conceivable that the third limiting portion 262 can also be set as a second convex portion, and the fourth limiting portion 2511 can be set as a second groove extending in the axial direction.

According to another specific embodiment of the present invention, as shown in FIG. 3 to FIG. 6 , the casing 200 is provided with a through hole, and the liquid seal 230 is penetrated on the casing 200 through the through hole. The operating part and the sealing part located in the housing 200, the sealing part seals and destroys the upper surface of the liquid storage part 220, and the liquid sealing member 230 has a first position and a second position. When the detection system is not in use, the liquid sealing member 230 is located in Fig. In the first position shown in FIG. 3, the liquid seal 230 can be moved to the second position shown in FIG. 4 through the operation part, and the destruction liquid storage part 220 can be opened. During the whole process, the liquid seal 230 and the casing 200 are always sealed Connected, the cavity 210 is always in a sealed state, and the amplification product will not leak.

According to another specific embodiment of the present invention, the lower end of the positioning portion 251 is provided with a third convex portion 2512, and the liquid sealing member 230 is provided with a corresponding third groove. Before the piercing mechanism 250 moves upward, the third protrusion 2512 is disposed in the third groove. Due to the cooperation between the third convex portion 2512 and the third groove, the liquid seal 230 is always in the first position and cannot be moved to the second position. Insert the positioning portion 251 into the nucleic acid amplification tube 100 and rotate to lift the puncturing mechanism 250 Afterwards, the third protruding portion 2512 also moves upwards and disengages from the third groove. At this time, the inspector can move the liquid seal 230 from the first position to the second position through the operation part, open the destruction liquid storage part 220 , and the chromatography test paper 240 can be in contact with the destruction liquid in the destruction liquid storage part 220 .

According to another specific embodiment of the present invention, an elastic depression structure 280 is disposed above the chromatography test paper 240 . When the liquid seal 230 is opened, the elastic depression structure 280 presses at least part of the chromatography test paper 240 into the destruction liquid storage part 220 . . The elastic pressing structure 280 is not particularly limited, and may be a spring or an elastic sheet.

Further, the specific structure for realizing the sealing of the cavity 210 can refer to any existing method in the prior art, which is not repeated in the present invention. For example, the nucleic acid solution adding area 260 can be set to match the nucleic acid amplification tube 100 by setting the shape. After the nucleic acid amplification tube 100 is inserted into the nucleic acid solution adding area 260 , the surfaces of the two are abutted, and the cavity 210 is sealed. According to another specific embodiment of the present invention, in order to more effectively prevent the expansion product from leaking into the air, a sealing ring 140 made of elastomer can also be provided at the insertion end of the cylindrical channel, or the side of the nucleic acid amplification tube 100 A sealing ring 140 made of elastomer is provided outside the pipe wall 110 .

According to another specific embodiment of the present invention, the detection result observation area 270 is made of transparent material.

Although the present invention has been illustrated and described by referring to some preferred embodiments of the present invention, those of ordinary skill in the art should understand that the above content is a further detailed description of the present invention in conjunction with specific embodiments, and it cannot be assumed that Embodiments of the present invention are limited only by these descriptions. Those skilled in the art may make various changes in form and details, including making several simple deductions or substitutions, without departing from the spirit and scope of the present invention.

Claims (7)

1. A nucleic acid amplification tube, characterized in that, comprising a nucleic acid amplification tube body and a nucleic acid amplification tube cover, the lower end of the nucleic acid amplification tube body is sealed, and the upper end is open, and the nucleic acid amplification tube cover seals the nucleic acid The opening of the amplification tube body forms a closed nucleic acid solution storage cavity, and the nucleic acid amplification tube is provided with a temperature display area. 2 . The nucleic acid amplification tube according to claim 1 , wherein the temperature display area comprises a reversible temperature test paper. 3 . 3. The nucleic acid amplification tube according to claim 1, wherein the temperature display area comprises a first temperature display area and a second temperature display area, the first temperature display area and the second temperature display area The regions are respectively coated with reversible thermochromic materials with different discoloration temperatures. 4 . The nucleic acid amplification tube according to claim 1 , wherein the temperature display area is provided on the nucleic acid amplification tube cover. 5 . 5 . The nucleic acid amplification tube according to claim 1 , wherein the temperature display area is provided on the side wall of the nucleic acid amplification tube body. 6 . 6. A fully enclosed nucleic acid detection system, characterized in that it comprises a housing and the nucleic acid amplification tube according to any one of claims 1-5, wherein a cavity is provided in the housing, and an upper surface of the housing is provided with a cavity. Nucleic acid solution addition area, A chromatographic test paper and a puncturing mechanism are arranged in the cavity below the nucleic acid solution addition area in sequence from bottom to top, and the nucleic acid solution addition area is set for insertion of a nucleic acid amplification tube, and the nucleic acid amplification tube is inserted in the nucleic acid amplification tube. After the extension tube is inserted into the nucleic acid solution addition area, the cavity is sealed, the puncturing mechanism pierces the nucleic acid amplification tube, and the nucleic acid solution flows into the cavity. 7 . The fully enclosed nucleic acid detection system according to claim 6 , wherein the cavity is further provided with a destruction liquid storage part, and the destruction liquid storage part can be opened to allow the destruction liquid to flow into the cavity. 8 . .

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