CN111808731A - Nucleic acid amplification tube and totally-enclosed nucleic acid detection system - Google Patents
Nucleic acid amplification tube and totally-enclosed nucleic acid detection system Download PDFInfo
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- CN111808731A CN111808731A CN202010647413.4A CN202010647413A CN111808731A CN 111808731 A CN111808731 A CN 111808731A CN 202010647413 A CN202010647413 A CN 202010647413A CN 111808731 A CN111808731 A CN 111808731A
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5082—Test tubes per se
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
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- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0672—Integrated piercing tool
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
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Abstract
The invention provides a nucleic acid amplification tube and a totally-enclosed nucleic acid detection system. By adopting the technical scheme, when the nucleic acid amplification reaction is carried out in certain poverty or laggard areas and constant-temperature equipment cannot be obtained in time, the nucleic acid amplification tube can be directly placed in a heat-insulating container, hot water and cold water are added, the amount of the hot water or the cold water is adjusted to be an appropriate reaction temperature through the temperature displayed by the temperature display area, and the nucleic acid amplification reaction can be carried out on site without additional constant-temperature water bath equipment for instant detection.
Description
Technical Field
The invention relates to the field of detection, in particular to a nucleic acid amplification tube and a totally-enclosed nucleic acid detection system.
Background
Nucleic acid diagnostics is one of the most active segments of the future IVD (in vitro diagnostics) industry. The increasing of the prevention and treatment of infectious diseases, the popularization of blood screening nucleic acid detection and the development of individualized medical treatment in China are the main motivations for the development of nucleic acid diagnosis in China. Under the promotion of the factors, the future speed increase of domestic nucleic acid diagnosis is 25-30%, and obviously exceeds the average speed increase of domestic IVD industry. On one hand, the nucleic acid diagnosis benefits large medical centers and realizes early, rapid, specific and high-throughput detection of pathogens, genetic diseases and the like.
POCT (Point-of-care testing), which is a new subdivision industry of In Vitro Diagnosis (IVD), is a new method for analyzing samples immediately on a sampling site, saving complex processing procedures of samples during laboratory testing and quickly obtaining testing results. The main criteria for POCT are that no fixed detection site is required, that the reagents and instruments are portable and that they can be operated in a timely manner. POCT plays the role of a laboratory, does not need traditional hospital laboratory equipment, and can serve patients in all directions within 24 hours without the limitation of time and place.
Particularly, for some poor or laggard areas, the traditional Chinese medicine preparation is a serious disaster area abused by infectious diseases due to poor quality, poor sanitary conditions, low sanitary consciousness, malnutrition and the like. The infectious diseases have high incidence and death rate, and the high treatment cost is difficult to be borne by the ordinary families. However, in these areas, advanced infectious disease detection methods are not widespread, mainly because of the difficulty in supplying power in most areas and the inability to operate large instruments; the cost of large-scale medical equipment and the corresponding cost of maintenance equipment cannot be borne; site restriction; the patient cannot afford the high examination cost, etc. The amplification reaction in the nucleic acid amplification tube needs to be carried out in a specific temperature range and in a specific closed laboratory, so that sample leakage and environmental pollution are avoided, and in the region, detection personnel can hardly obtain temperature constant-temperature equipment and the closed laboratory, so that the on-site detection is not possible, and the instantaneity of nucleic acid detection is limited.
Therefore, it is an urgent problem in the art to provide a method for detecting nucleic acid in real time.
Disclosure of Invention
The invention aims to solve the problem that the nucleic acid detection cannot be carried out on site in the prior art. In order to solve the problems, the invention discloses a nucleic acid amplification tube with a brand-new structure and a totally-enclosed nucleic acid detection system, which can be used for detecting nucleic acid at any time and greatly promote the application of nucleic acid detection in poor or laggard areas.
The invention discloses a nucleic acid amplification tube, which comprises a nucleic acid amplification tube body and a nucleic acid amplification tube cover, wherein the lower end of the nucleic acid amplification tube body is sealed, the upper end of the nucleic acid amplification tube body is opened, the nucleic acid amplification tube cover seals the opening of the nucleic acid amplification tube body to form a closed nucleic acid solution storage cavity, and a temperature display area is arranged on the nucleic acid amplification tube.
By adopting the technical scheme, when the nucleic acid amplification reaction is carried out in certain poor or laggard areas and constant temperature equipment cannot be obtained in time, the nucleic acid amplification tube can be directly placed in a container, hot water and cold water are added, the temperature display area can display the current temperature of the nucleic acid amplification tube, the amount of the hot water or the cold water is adjusted to the proper temperature of the nucleic acid amplification reaction through the temperature displayed by the temperature display area, no additional constant temperature water bath equipment is needed, the nucleic acid solution in the nucleic acid amplification tube can carry out the nucleic acid amplification reaction on the spot, and the detection can be carried out immediately after the reaction is finished.
According to another embodiment of the present invention, the temperature display area comprises a reversible temperature test paper.
According to another specific embodiment of the present invention, the temperature display area includes a first temperature display area and a second temperature display area, and the first temperature display area and the second temperature display area are respectively coated with reversible temperature-sensitive color-changing materials having different color-changing temperatures.
According to another embodiment of the invention, the temperature display area is arranged on the tube cover or the temperature display area is arranged on the side tube wall.
The invention also discloses a totally-enclosed nucleic acid detection system, which comprises a shell and any one of the nucleic acid amplification tubes, wherein a cavity is arranged in the shell, a nucleic acid solution adding area is arranged on the upper surface of the shell,
the nucleic acid solution adding area is arranged for inserting the nucleic acid amplification tube, the cavity is sealed after the nucleic acid amplification tube is inserted into the nucleic acid solution adding area, the puncturing mechanism punctures the nucleic acid amplification tube, and the nucleic acid solution flows into the cavity.
According to another embodiment of the present invention, a destructive liquid reservoir is provided in the cavity, and the destructive liquid reservoir is openable to allow the destructive liquid to flow into the cavity.
Drawings
The invention will be described in further detail with reference to the following figures and detailed description:
FIG. 1 is a sectional view of a nucleic acid amplification tube provided by the present invention;
FIG. 2 is a schematic structural view of a nucleic acid amplification tube according to the present invention;
FIG. 3 is a sectional view of the nucleic acid amplification tube and the detection system of the present invention in a state of being coupled to each other;
FIG. 4 is a sectional view of the nucleic acid amplification tube and the detection system according to another embodiment of the present invention;
FIG. 5 is a schematic structural view of a nucleic acid amplification tube and a detection system provided by the present invention in a state of being mated with each other;
FIG. 6 is an exploded view of a nucleic acid amplification tube and detection system provided by the present invention;
FIG. 7 is a schematic diagram of the lancing mechanism provided by the present invention;
FIG. 8 is a schematic diagram of the lancing mechanism provided by the present invention.
Reference numerals:
nucleic acid amplification tube 100
First convex part 111
Pipe cap 130
Nucleic acid solution storage chamber 140
Seal ring 150
Destructive liquid storage part 220
Positioning part 251
Nucleic acid solution addition region 260
Third position-limiting part 262
Test result observation area 270
Elastic pressing structure 280
Detailed Description
The following description of the embodiments of the present invention is provided for illustrative purposes, and other advantages and effects of the present invention will become apparent to those skilled in the art from the present disclosure. While the invention will be described in conjunction with the preferred embodiments, it is not intended that features of the invention be limited to these embodiments. On the contrary, the invention is described in connection with the embodiments for the purpose of covering alternatives or modifications that may be extended based on the claims of the present invention. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. The invention may be practiced without these particulars. Moreover, some of the specific details have been left out of the description in order to avoid obscuring or obscuring the focus of the present invention. It should be noted that the embodiments and features of the embodiments may be combined with each other without conflict.
In the description of the present embodiment, it should be noted that the terms "upper", "lower", "inner", "bottom", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings or orientations or positional relationships that are conventionally placed when the products of the present invention are used, and are only used for convenience in describing the present invention and simplifying the description, but do not indicate or imply that the devices or elements indicated must have specific orientations, be configured in specific orientations, and operate, and thus, should not be construed as limiting the present invention.
The terms "first," "second," "third," and the like are used solely to distinguish one from another and are not to be construed as indicating or implying relative importance.
In the description of the present embodiment, it should be further noted that, unless explicitly stated or limited otherwise, the terms "disposed," "connected," and "connected" are to be interpreted broadly, e.g., as a fixed connection, a detachable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present embodiment can be understood in specific cases by those of ordinary skill in the art.
In order to make the objects, technical solutions and advantages of the present invention more apparent, embodiments of the present invention will be described in detail with reference to the accompanying drawings.
As shown in FIGS. 1 and 2, the present invention discloses a nucleic acid amplification tube 100, which comprises a nucleic acid amplification tube body and a nucleic acid amplification tube cap 130, wherein the lower end of the nucleic acid amplification tube body is sealed, the upper end of the nucleic acid amplification tube body is open, the nucleic acid amplification tube body comprises a side tube wall 110 and a lower tube wall 120, the nucleic acid amplification tube cap 130 seals the opening of the nucleic acid amplification tube body to form a closed nucleic acid solution storage cavity 140, and the nucleic acid amplification tube body is further provided with a temperature display region 160.
By adopting the technical scheme, when the nucleic acid amplification reaction is carried out in certain poverty or laggard areas and constant-temperature equipment cannot be obtained in time, the nucleic acid amplification tube can be directly placed in a heat-insulating container, hot water and cold water are added, the amount of the hot water or the cold water is adjusted to be an appropriate reaction temperature through the temperature displayed by the temperature display area 160, and the nucleic acid amplification reaction can be carried out on site without additional constant-temperature water bath equipment.
According to another embodiment of the present invention, the nucleic acid amplification tube 100 of the present invention is a special nucleic acid amplification tube, and the side wall 110 of the nucleic acid amplification tube 100 may be made of a series of materials, preferably a material with good thermal conductivity, high strength and good fluidity, such as metal, alloy, thermal conductive plastic and organic composite material, and has a height of 1-3cm, preferably 2cm, and a general shape similar to that of a general nucleic acid amplification tube, but with some differences.
According to another embodiment of the present invention, the temperature display area 160 may be a reversible temperature test paper. The reversible temperature test strip may be selected appropriately according to the temperature of the nucleic acid amplification reaction. For example, when the temperature of the nucleic acid amplification reaction is 38 ℃, a reversible temperature test paper of 0-50 ℃ of THERMAX can be selected; if 63 ℃ is required for the nucleic acid amplification reaction, a reversible temperature test strip of THERMAX at 50-100 ℃ may be selected. In addition, the special temperature test paper can be customized to suppliers according to actual conditions.
According to another embodiment of the present invention, the temperature display region 160 includes a first temperature display region and a second temperature display region, and the first temperature display region and the second temperature display region are respectively coated with reversible temperature-sensitive color-changing materials having different color-changing temperatures. The color-changing temperature of the temperature-sensitive color-changing material can be set according to the actual situation, and the specific reversible temperature-sensitive color-changing material can be a commercially available product. Aiming at a certain amplification reaction, if the reaction temperature is required to be between a first temperature T1 and a second temperature T2, two temperature-sensitive color-changing materials can be selectively coated on the surface of the nucleic acid amplification tube 100, wherein the color-changing temperature of the first temperature-sensitive color-changing material is the first temperature T1, and the color-changing temperature of the second temperature-sensitive color-changing material is the second temperature T2. Thus, when the first temperature sensitive material is discolored and the second temperature sensitive material is not discolored during the amplification reaction, it is indicated that the temperature is suitable for the amplification reaction in the nucleic acid amplification tube 100. In this way, the nucleic acid amplification tube 100 can be directly placed in a thermos flask, and the temperature of water in the thermos flask can be controlled by adjusting the amount of cold and hot water, so that the amplification reaction can be maintained, and the sample can be detected at any time and any place without using a thermostat.
For example, if the optimal reaction temperature is about 38 ℃, the temperature-sensitive paint can be selected from 2 temperatures, which are respectively greater than 38 and less than 38, preferably 37 and 39 ℃, if the optimal reaction temperature is 63 ℃, the temperature-sensitive paint can be selected from 62 and 64, the shape of the temperature-sensitive paint can be any, but preferably arabic numerals corresponding to the temperature, such as 38-degree discolored temperature-sensitive material, shown as "38". This allows the temperature of the nucleic acid amplification tube 100 to be more directly reacted.
Further, when the nucleic acid amplification tube 100 is used in amplification reaction systems having a plurality of different temperatures, a plurality of temperature-sensitive color-changing materials may be provided.
According to another embodiment of the present invention, in order to facilitate the nucleic acid amplification tube 100 to be punctured by the puncturing mechanism 250 after being inserted into the totally enclosed nucleic acid detecting system, a cut mark may be provided on the lower tube wall 120 of the nucleic acid amplification tube 100, and when the puncturing mechanism 250 punctures the lower tube wall 120 of the nucleic acid amplification tube 100, the lower tube wall 120 of the nucleic acid amplification tube 100 is ruptured along the position of the cut mark; or the lower tube wall 120 may be made thinner than other portions of the nucleic acid amplification tube 100, so that the puncturing mechanism 250 punctures the nucleic acid amplification tube 100.
Further, since the lower tube wall 120 of the nucleic acid amplification tube 100 is provided with a cut or the lower tube wall 120 is made thinner, the nucleic acid amplification tube 100 may be damaged along the cut or the thinner portion during the picking and placing process before being inserted into the totally enclosed nucleic acid detecting system. Therefore, according to another embodiment of the present invention, the lower tube wall 120 of the nucleic acid amplification tube 100 is provided with a concave portion 121 recessed toward the tube cover 130, and the cut is provided in the concave portion 121; alternatively, only the wall of the nucleic acid amplification tube 100 in the recess 121 may be thin, and preferably at least the central portion of the lower wall 120 of the nucleic acid amplification tube 100 is thin, so that the damage of the nucleic acid amplification tube 100 during the taking and placing process can be effectively avoided.
According to another embodiment of the present invention, the general nucleic acid amplification tube 100 is an open-lid structure, that is, the tube lid 130 is an openable lid, and after the sample to be amplified and the reagents related to the amplification reaction system are placed in the nucleic acid amplification tube 100, the lid is closed to achieve sealing. In addition, the upper portion of the nucleic acid amplification tube 100 may also be directly a closed structure, i.e., the tube cover 130 and the side tube wall 110 are fixedly connected, or even directly integrally formed, and cannot be opened. When in use, the syringe with the fine needle pierces the cap 130 of the nucleic acid amplification tube 100, injects the reaction system, and then seals the breach with a sealing film or a wax droplet having a higher melting point, thereby achieving better sealing of the nucleic acid amplification tube 100.
According to another embodiment of the present invention, the temperature display area 160 is provided on the tube cover or the temperature display area 160 is provided on the side tube wall from the viewpoint of easy observation.
According to another embodiment of the present invention, one or more first protrusions 111 are disposed on the outer surface of the side tube wall 110, and if there are a plurality of first protrusions 111, the plurality of first protrusions 111 may be disposed on the same cross section of the side tube wall 110, at least a portion of the side tube wall 110 is a cylindrical side tube wall, and an external thread (not shown) is disposed on the outer surface of the cylindrical side tube wall and is disposed below the first protrusions 111.
The first protrusion 111 is not particularly limited as long as it can fit into the first groove 261 to limit the position of the nucleic acid amplification tube 100 relative to the cylindrical channel in the axial direction, and the first protrusion 111 may be a protrusion, a protrusion extending in the circumferential direction, or a complete circular protrusion ring. From the viewpoint of more effectively and stably restricting the position of the nucleic acid amplification tube 100 in the axial direction, the first convex portion 111 is preferably a complete circular convex ring, or a plurality of convex points or convex strips uniformly distributed along the circumferential direction of the side tube wall 110.
According to another embodiment of the present invention, as shown in fig. 1 and 2, the first protrusion 111 of the nucleic acid amplification tube 100 side tube wall 110 is a circular protrusion ring, and the nucleic acid amplification tube 100 side tube wall 110 under the first protrusion 111 is a cylindrical side tube wall provided with an external thread that engages with the internal thread of the positioning portion 251 of the puncturing mechanism 250.
Further, as shown in FIGS. 3 to 6, the present invention also discloses a totally enclosed nucleic acid detecting system, which comprises a housing 200 and any one of the nucleic acid amplification tubes 100, wherein a cavity 210 is provided in the housing 200, a nucleic acid solution adding region 260 is provided on the upper surface of the housing 200,
the chromatography test paper 240 and the puncturing mechanism 250 are sequentially arranged in the cavity below the nucleic acid solution adding region 260 from bottom to top, the nucleic acid solution adding region 260 is provided for inserting the nucleic acid amplification tube 100, after the nucleic acid amplification tube 100 is inserted into the nucleic acid solution adding region 260, the cavity 210 is sealed, the puncturing mechanism 250 punctures the lower tube wall 130 of the nucleic acid amplification tube 100, and the nucleic acid solution flows into the cavity 210.
In some poor or laggard areas, the nucleic acid amplification tube 100 provided by the invention can be used for immediately completing the nucleic acid amplification reaction, and then the nucleic acid amplification tube 100 can be inserted into the nucleic acid solution adding area 260, so that the nucleic acid detection can be quickly completed, and because the nucleic acid amplification tube 100 and the nucleic acid solution adding area 260 are in sealed connection, the sample can be effectively prevented from volatilizing into the air and polluting the environment, and the nucleic acid sample to be detected can be immediately detected without being conveyed to a special closed laboratory for detection.
According to another embodiment of the present invention, a destructive liquid storage part 220 is further provided in the cavity 210, and the destructive liquid storage part 220 is opened to allow the destructive liquid to flow into the cavity 210. The nucleic acid destroying reagent is adopted to remove the residual nucleic acid in the totally closed nucleic acid detection system, thereby further avoiding possible pollution in the subsequent process.
According to another embodiment of the present invention, a cavity 210 is formed in the housing 200, a destructive liquid storage unit 220, a chromatography test paper 240 and a puncturing mechanism 250 are sequentially disposed in the cavity 210 from bottom to top, an opening is formed on the upper surface of the destructive liquid storage unit 220, the opening is sealed by a liquid sealing member 230, the liquid sealing member 230 can be opened, and a nucleic acid damaging reagent, such as a sodium hypochlorite solution or a commercial DNA detergent, can be stored in the destructive liquid storage unit 220.
The upper surface of the housing 200 is provided with a nucleic acid solution adding region 260 and a detection result observing region 270, the nucleic acid solution adding region 260 comprises a cylindrical channel including an insertion end and a non-insertion end, the insertion end is configured to be inserted with the nucleic acid amplification tube 100, the insertion end is shaped to fit with the nucleic acid amplification tube 100 to seal the cavity 210, the insertion end of the cylindrical channel is provided with a first groove 261 having a ring shape, when the nucleic acid amplification tube 100 is inserted into the cylindrical channel, the first protrusion 111 and the first groove 261 cooperate with each other to restrict the nucleic acid amplification tube 100 from moving in the axial direction with respect to the cylindrical channel,
as shown in fig. 7 and 8, the puncturing mechanism 250 includes a cylindrical positioning portion 251, a puncturing portion 252 located on the central axis of the positioning portion 251, the puncturing mechanism 250 further includes a fluid channel 253, a third position-limiting portion 262 is provided at the non-insertion end of the cylindrical channel, a fourth position-limiting portion 2511 is provided at the positioning portion 251, the third position-limiting portion 262 and the fourth position-limiting portion 2511 cooperate with each other to limit the puncturing mechanism 250 from rotating around the central axis of the positioning portion 251, an internal thread (not shown) is provided on the inner surface of the positioning portion 251, and an external thread cooperating with the internal thread is provided on the outer surface of the nucleic acid amplification tube 100.
When the nucleic acid amplification tube 100 is inserted into the cylindrical passage and the nucleic acid amplification tube 100 is rotated, as shown in FIG. 3, since the first protrusion 111 is engaged with the annular first groove 261, the nucleic acid amplification tube 100 can only be rotated and cannot be inserted inwardly with respect to the cylindrical passage. And as the nucleic acid amplification tube 100 rotates, because the external thread on the tube wall 110 of the nucleic acid amplification tube 100 side matches with the internal thread on the positioning part 251, the positioning part 251 will drive the piercing part 252 to move to the position shown in fig. 4 toward the nucleic acid amplification tube 100 (the liquid seal 230 is already opened in fig. 4, but the liquid seal 230 cannot be opened before detection is not completed, here, only fig. 4 is used to show the relative position relationship between the piercing mechanism 250 and the nucleic acid amplification tube 100), so as to pierce the nucleic acid amplification tube 100, and the nucleic acid solution flows into the cavity 210 from the fluid channel 253. The chromatographic test paper 240 in the cavity 210 can detect the sample, and the inspector can observe the detection result through the detection result observation area 270.
By adopting the technical scheme, the cavity 210 is always sealed in the detection process, so that the amplification product is prevented from leaking outwards. Further, after recording the detection result, as shown in FIG. 4, the liquid seal 230 may be opened, and the chromatographic test paper 240 may react with the nucleic acid-disrupting reagent to completely remove the residual nucleic acid in the totally enclosed nucleic acid detection system. Thus, even if the nucleic acid amplification tube 100 is inadvertently detached or the totally enclosed nucleic acid detection system is damaged during the subsequent process, the inside is exposed and no contamination is caused.
In addition, in the prior art in which the nucleic acid amplification tube 100 is inserted downward while the piercing mechanism 250 is kept stationary, since the gas inside the housing 200 is compressed, the amplification product may leak from the gap where the nucleic acid amplification tube 100 and the housing 200 are connected, thereby causing contamination. By the totally-enclosed nucleic acid detection system with a brand-new structure provided by the invention, the device allows the nucleic acid amplification tube 100 to be kept still in the axial direction, but the puncture mechanism 250 is moved upwards by rotating to puncture the nucleic acid amplification tube 100, the air pressure in the shell 200 is unchanged during the puncturing process, the aerosol of the amplification product does not leak to the outside, and the pollution can be better avoided. And the nucleic acid amplification tube 100 is connected with the shell 200 through threads, so that the connection structure is more stable and is not easy to fall off, and the possibility of pollution caused by exposure of amplification products is further avoided. And finally, a nucleic acid destroying reagent is adopted to remove the residual nucleic acid in the totally closed nucleic acid detection system, thereby further avoiding possible pollution in the subsequent process.
According to another embodiment of the present invention, the third position-limiting portion 262 is a second groove extending along the axial direction, and the fourth position-limiting portion 2511 is a second protrusion. The second convex part can be a convex point or a convex strip extending along the axial direction; and the second groove has a length that allows the puncturing mechanism 250 to move upward to a position where the nucleic acid amplification tube 100 is punctured. Before the nucleic acid amplification tube 100 is inserted into the positioning part 251 and starts to rotate, the second convex part is located at the lowermost end of the second groove, and as the nucleic acid amplification tube 100 rotates, the entire puncturing mechanism 250 moves upward, and the second convex part moves upward along the second groove until the puncturing mechanism 250 punctures the lower tube wall 120 of the nucleic acid amplification tube 100.
Further, from the viewpoint of more reliability, a plurality of second protrusions and second grooves extending in the axial direction in cooperation therewith may be further provided, and preferably, the plurality of second protrusions and the second grooves extending in the axial direction in cooperation therewith are uniformly distributed in the circumferential direction of the cylindrical passage.
It is easily conceivable that the third stopper portion 262 may be provided as a second convex portion and the fourth stopper portion 2511 may be provided as a second concave portion extending in the axial direction.
According to another embodiment of the present invention, as shown in fig. 3 to 6, a through hole is provided on the housing 200, the liquid sealing member 230 is disposed on the housing 200 through the through hole, the liquid sealing member 230 includes an operation portion located outside the housing 200 and a sealing portion located inside the housing 200, the sealing portion seals the upper surface of the destructive liquid storage portion 220, and the liquid sealing member 230 has a first position and a second position, when the detection system is not used, the liquid sealing member 230 is located at the first position shown in fig. 3, the liquid sealing member 230 can be moved to the second position shown in fig. 4 by the operation portion, the destructive liquid storage portion 220 is opened, the liquid sealing member 230 and the housing 200 are always in a sealing connection, the cavity 210 is always in a sealing state, and the amplification product does not leak.
According to another embodiment of the present invention, a third protrusion 2512 is disposed at the lower end of the positioning part 251, and a corresponding third groove is disposed on the liquid sealing member 230. Third protrusion 2512 is disposed within third recess before lancing mechanism 250 is not moved upward. Due to the cooperation between the third protrusion 2512 and the third groove, the liquid seal 230 is always in the first position and cannot be moved to the second position, and after the nucleic acid amplification tube 100 is inserted into the positioning part 251 and the lift-and-puncture mechanism 250 is rotated, the third protrusion 2512 is also moved upward and separated from the third groove. At this time, the operator may move the liquid seal 230 from the first position to the second position by the operation portion, open the destructive liquid reservoir 220, and contact the chromatographic test paper 240 with the destructive liquid in the destructive liquid reservoir 220.
According to another embodiment of the present invention, an elastic pressing structure 280 is disposed above the chromatographic test strip 240, and when the liquid sealing member 230 is opened, the elastic pressing structure 280 presses at least a portion of the chromatographic test strip 240 into the destructive liquid storage portion 220. The elastic pressing structure 280 is not particularly limited, and may be a spring or a leaf spring.
Further, the specific structure for sealing the cavity 210 may refer to any manner known in the art, and is not described herein, for example, the nucleic acid solution adding region 260 may be configured to match the nucleic acid amplification tube 100 by configuring the shape thereof, and after the nucleic acid amplification tube 100 is inserted into the nucleic acid solution adding region 260, the surfaces of the two are attached to each other, so as to seal the cavity 210. According to another embodiment of the present invention, in order to more effectively prevent the expansion product from leaking into the air, a sealing ring 140 made of an elastomer may be further provided at the insertion end of the cylindrical channel, or a sealing ring 140 made of an elastomer may be provided outside the side tube wall 110 of the nucleic acid amplification tube 100.
According to another embodiment of the present invention, the test result observation region 270 is made of a transparent material.
While the invention has been shown and described with reference to certain preferred embodiments thereof, it will be understood by those skilled in the art that the foregoing is a more detailed description of the invention, taken in conjunction with the specific embodiments thereof, and that no limitation of the invention is intended thereby. Various changes in form and detail, including simple deductions or substitutions, may be made by those skilled in the art without departing from the spirit and scope of the invention.
Claims (7)
1. A nucleic acid amplification tube is characterized by comprising a nucleic acid amplification tube body and a nucleic acid amplification tube cover, wherein the lower end of the nucleic acid amplification tube body is sealed, the upper end of the nucleic acid amplification tube body is opened, the opening of the nucleic acid amplification tube body is sealed by the nucleic acid amplification tube cover to form a closed nucleic acid solution storage cavity, and a temperature display area is arranged on the nucleic acid amplification tube.
2. The nucleic acid amplification tube of claim 1, wherein the temperature display region comprises a reversible temperature test paper.
3. The nucleic acid amplification tube of claim 1, wherein the temperature display region comprises a first temperature display region and a second temperature display region, and the first temperature display region and the second temperature display region are coated with reversible temperature-sensitive color-changing materials having different color-changing temperatures, respectively.
4. The nucleic acid amplification tube of claim 1, wherein the temperature display region is provided on the nucleic acid amplification tube cover.
5. The nucleic acid amplification tube according to claim 1, wherein the temperature display region is provided on a side tube wall of the nucleic acid amplification tube body.
6. A totally enclosed nucleic acid detecting system comprising a housing and the nucleic acid amplification tube according to any one of claims 1 to 5, wherein the housing has a cavity therein, the upper surface of the housing has a nucleic acid solution addition region,
the nucleic acid solution adding device is characterized in that a chromatography test paper and a puncturing mechanism are sequentially arranged in a cavity below the nucleic acid solution adding area from bottom to top, the nucleic acid solution adding area is set to be used for inserting a nucleic acid amplification tube, the cavity is sealed after the nucleic acid amplification tube is inserted into the nucleic acid solution adding area, the puncturing mechanism punctures the nucleic acid amplification tube, and nucleic acid solution flows into the cavity.
7. The totally enclosed nucleic acid detecting system according to claim 6, wherein a destructive liquid reservoir is further provided in the cavity, and the destructive liquid reservoir is openable to allow the destructive liquid to flow into the cavity.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113846004A (en) * | 2021-10-29 | 2021-12-28 | 广东粤港澳大湾区国家纳米科技创新研究院 | Rotary type nucleic acid amplification product detection device and detection method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113846004A (en) * | 2021-10-29 | 2021-12-28 | 广东粤港澳大湾区国家纳米科技创新研究院 | Rotary type nucleic acid amplification product detection device and detection method |
| CN113846004B (en) * | 2021-10-29 | 2024-03-15 | 武汉纳达康生物科技有限公司 | Rotary nucleic acid amplification product detection device and detection method |
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