CN111876320A - Chromatography test paper detection device - Google Patents
Chromatography test paper detection device Download PDFInfo
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- CN111876320A CN111876320A CN202010648508.8A CN202010648508A CN111876320A CN 111876320 A CN111876320 A CN 111876320A CN 202010648508 A CN202010648508 A CN 202010648508A CN 111876320 A CN111876320 A CN 111876320A
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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Abstract
The invention provides a chromatographic test paper detection device, which comprises a shell, a destruction operating part, chromatographic test paper, a puncture mechanism and a sample solution pipe, wherein the destruction operating part comprises an operating part positioned outside the shell and a sealing part positioned in the shell, when the detection device is not used, the sealing part seals the upper surface of a liquid storage tank, the liquid storage tank is opened through the operating part, and the destruction operating part is always in sealing connection with the shell. By adopting the technical scheme, after the chromatographic test paper is detected, the operating part is pulled from the outside of the shell, the damage operating part moves to the second position, the upper surface of the damage liquid storage tank is opened, so that the damage liquid in the damage liquid storage tank reacts with the sample solution on the chromatographic test paper and in the sample solution pipe, and the residual nucleic acid in the chromatographic test paper detection device is completely removed. Therefore, even if the sample solution pipe is accidentally dropped in the subsequent process or the chromatography test paper detection device is damaged, the internal exposure is caused, and no pollution is caused.
Description
Technical Field
The invention relates to the field of detection, in particular to a chromatographic test paper detection device.
Background
Nucleic acid diagnostics is one of the most active segments of the future IVD (in vitro diagnostics) industry. The increasing of the prevention and treatment of infectious diseases, the popularization of blood screening nucleic acid detection and the development of individualized medical treatment in China are the main motivations for the development of nucleic acid diagnosis in China. Under the promotion of the factors, the future speed increase of domestic nucleic acid diagnosis is 25-30%, and obviously exceeds the average speed increase of domestic IVD industry. On one hand, the nucleic acid diagnosis benefits large medical centers and realizes early, rapid, specific and high-throughput detection of pathogens, genetic diseases and the like.
POCT (Point-of-care testing), which is a new subdivision industry of In Vitro Diagnosis (IVD), is a new method for analyzing samples immediately on a sampling site, saving complex processing procedures of samples during laboratory testing and quickly obtaining testing results. The main criteria for POCT are that no fixed detection site is required, that the reagents and instruments are portable and that they can be operated in a timely manner. POCT plays the role of a laboratory, does not need traditional hospital laboratory equipment, and can serve patients in all directions within 24 hours without the limitation of time and place.
However, these nucleic acid amplification methods have a problem that the amplification products are easily cross-contaminated, and false positive signals generated by product contamination may cause erroneous interpretation of the detection results. Cross-contamination between samples is often seen during target nucleic acid amplification procedures, and contamination may result from known or unknown positive species introduced during negative sample processing, which causes false positive reactions through air contamination or aerosols.
In the prior art, a series of methods have been developed to prevent cross contamination of amplification products, and for example, reference 1(CN105199940A) discloses a portable anti-contamination gene detection method and apparatus, by which a nucleic acid amplification tube containing an amplification product can be sealed in an apparatus and then the nucleic acid amplification tube is punctured to perform detection. Prevent the pollution of nucleic acid amplification products and avoid false positive. However, in this apparatus, since the nucleic acid amplification tube is sealed after being placed in the apparatus, the puncturing operation is difficult, and the amplification product after the completion of the test remains in the apparatus, and if the nucleic acid amplification tube is broken, the amplification product may also diffuse into the air, causing a false positive reaction.
Further, while reference 2(CN203241416U) discloses a closed type chromatography strip plastic cartridge, and reference 3(CN205574438U) discloses a sealed tube assembly including a tube breaking mechanism, these detection devices are convenient to operate, but the amplification product remains in the device after detection, which may cause contamination.
Therefore, it is an urgent problem in the art to provide a nucleic acid detecting apparatus with less contamination possibility before and after detection.
Disclosure of Invention
The invention aims to solve the problems that a closed detection device in the prior art is inconvenient to operate and has pollution possibility after detection. In order to solve the problems, the invention discloses a chromatographic test paper detection device which is convenient to operate and can effectively prevent amplification products from diffusing into the air to cause false positive reaction.
In order to solve the problems, the invention discloses a chromatography test paper detection device which comprises a shell, a damage operating part, chromatography test paper, a puncture mechanism and a sample solution pipe, wherein a containing cavity is arranged in the shell, a damage liquid storage tank is arranged in the containing cavity, and the chromatography test paper is arranged above the damage liquid storage tank and can react with the sample solution in the sample solution pipe;
the shell is provided with a through hole, the damage operating part penetrates through the shell through the through hole, the damage operating part comprises an operating part positioned outside the shell and a sealing part positioned in the shell, the sealing part seals the upper surface of the liquid storage tank, the damage operating part is provided with a first position and a second position, when the detection device is not used, the damage operating part is positioned at the first position, the damage operating part can be moved to the second position through the operating part, the liquid storage tank is opened, and the damage operating part is always in sealing connection with the shell;
the puncture mechanism is arranged in the shell, a fluid channel is arranged on the puncture mechanism, and the puncture mechanism can puncture the bottom wall of the sample solution tube, so that the sample solution flows into the containing cavity from the fluid channel to react with the chromatography test paper.
By adopting the technical scheme, after the chromatographic test paper is detected, the operating part is pulled from the outside of the shell, the damage operating part moves to the second position, the upper surface of the damage liquid storage tank is opened, so that the damage liquid in the damage liquid storage tank reacts with the sample solution on the chromatographic test paper and in the sample solution pipe, and the residual nucleic acid in the chromatographic test paper detection device is completely removed. Therefore, even if the sample solution pipe is accidentally dropped in the subsequent process or the chromatography test paper detection device is damaged, the internal exposure is caused, and no pollution is caused.
According to another embodiment of the invention, the detection device further comprises a locking device having a first state and a second state, the locking device restricting movement of the destruction operating member from the first position to the second position when the locking device is in the first state, and the locking device releasing the restriction of movement of the destruction operating member when the locking device is in the second state.
According to another embodiment of the present invention, an elastic pressing structure is disposed above the chromatographic test strip, and presses at least a portion of the chromatographic test strip into the liquid storage tank when the liquid destruction operating member is opened.
According to another embodiment of the present invention, the upper surface of the housing is provided with a sample solution adding region, the sample solution adding region comprises a cylindrical first channel, an insertion end of the first channel is provided with a sealing ring made of an elastomer, or the side wall of the sample solution tube is externally provided with a sealing ring made of an elastomer.
According to another embodiment of the present invention, a sodium hypochlorite solution or a DNA detergent is stored in the liquid storage tank.
Drawings
The invention will be described in further detail with reference to the following figures and detailed description:
FIG. 1 is a perspective view of a chromatographic test strip detection device according to the present invention;
FIG. 2 is a cross-sectional view of the chromatographic test strip detection device provided by the present invention;
FIG. 3 is a cross-sectional view of a sample solution tube of the device for testing chromatography test paper according to the present invention in a punctured state;
FIG. 4 is an exploded view of the chromatographic test strip detection device provided by the present invention;
FIG. 5 is a schematic perspective view of a lancing mechanism in the chromatographic test strip detection device provided by the present invention;
FIG. 6 is a cross-sectional view of a sample solution tube provided by the present invention;
fig. 7 is a schematic structural view of a sample solution tube provided in the present invention.
Detailed Description
The following description of the embodiments of the present invention is provided for illustrative purposes, and other advantages and effects of the present invention will become apparent to those skilled in the art from the present disclosure. While the invention will be described in conjunction with the preferred embodiments, it is not intended that features of the invention be limited to these embodiments. On the contrary, the invention is described in connection with the embodiments for the purpose of covering alternatives or modifications that may be extended based on the claims of the present invention. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. The invention may be practiced without these particulars. Moreover, some of the specific details have been left out of the description in order to avoid obscuring or obscuring the focus of the present invention. It should be noted that the embodiments and features of the embodiments may be combined with each other without conflict.
In the description of the present embodiment, it should be noted that the terms "upper", "lower", "inner", "bottom", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings or orientations or positional relationships that are conventionally placed when the products of the present invention are used, and are only used for convenience in describing the present invention and simplifying the description, but do not indicate or imply that the devices or elements indicated must have specific orientations, be configured in specific orientations, and operate, and thus, should not be construed as limiting the present invention.
The terms "first," "second," and the like are used solely to distinguish one from another and are not to be construed as indicating or implying relative importance.
In the description of the present embodiment, it should be further noted that, unless explicitly stated or limited otherwise, the terms "disposed," "connected," and "connected" are to be interpreted broadly, e.g., as a fixed connection, a detachable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present embodiment can be understood in specific cases by those of ordinary skill in the art.
In order to make the objects, technical solutions and advantages of the present invention more apparent, embodiments of the present invention will be described in detail with reference to the accompanying drawings.
Referring to fig. 1 to 5, the present invention discloses a chromatography test paper detection device, which comprises a housing 200, a destruction operating member 230, a chromatography test paper 240, a puncture mechanism 250 and a sample solution tube 100, wherein the housing 200 is provided with a containing cavity 210, the containing cavity 210 is provided with a destruction liquid storage slot 220, the chromatography test paper 240 is arranged above the destruction liquid storage slot 220 and can react with the sample solution in the sample solution tube 100;
the casing 200 is provided with a through hole 201, the destruction operating member 230 is arranged on the casing 200 through the through hole 201, the destruction operating member 230 comprises an operating part 231 positioned outside the casing 200 and a sealing part 232 positioned in the casing 200, the sealing part 232 seals the upper surface of the destruction liquid storage tank 220, the destruction operating member 230 has a first position and a second position, when the detection device is not used, the destruction operating member 230 is positioned at the first position, the destruction operating member 230 can be moved to the second position through the operating part 231, the destruction liquid storage tank 220 is opened, and the destruction operating member 230 is always in sealing connection with the casing 200;
the puncturing mechanism 250 is disposed in the housing 200, and a fluid channel 253 is disposed on the puncturing mechanism 250, and the puncturing mechanism 250 can puncture the bottom wall 120 of the sample solution tube 100, so that the sample solution flows from the fluid channel 253 into the containing cavity 210 to react with the chromatography test paper 240.
That is, the chromatography test paper detection device mainly comprises a housing 200 and a sample solution tube 100, wherein a housing chamber 210 is arranged in the housing 200, a destructive liquid storage tank 220, a destructive operation member 230, chromatography test paper 240 and a puncture mechanism 250 are sequentially arranged in the housing chamber 210 from bottom to top, and the upper surface of the destructive liquid storage tank 220 is sealed by the destructive operation member 230. When the detection device is not used, the destruction operation member 230 can seal the destruction liquid storage tank 220, so as to prevent the destruction liquid in the destruction liquid storage tank 220 from reacting with the sample solution on the chromatographic test paper 240 and in the sample solution tube 100, and ensure the usability of the detection device, and at this time, the destruction operation member 230 is located at the first position. After the chromatographic test paper 240 is tested, the operation part 231 is pulled from the outside of the housing 200, so that the destruction operation member 230 is moved to the second position, the upper surface of the destruction liquid storage tank 220 is opened, the destruction liquid in the destruction liquid storage tank 220 reacts with the sample solution on the chromatographic test paper 240 and in the sample solution tube 100, and the nucleic acid remaining in the chromatographic test paper testing device is completely removed. Therefore, even if the sample solution tube 100 is accidentally dropped or the chromatography test paper detection device is damaged in the subsequent process, the internal exposure is caused, and no pollution is caused.
In this embodiment, the destruction liquid stored in the destruction liquid storage tank 220 may be a nucleic acid destruction reagent, such as a sodium hypochlorite solution or a solution of a commercial DNA detergent. In other embodiments, other solutions may be stored in the liquid storage tank as long as the solutions can react with the sample solution to prevent the sample solution from being contaminated.
Further, the upper surface of the housing 200 is provided with a sample solution adding region 260 and a detection result observing region 270, the sample solution adding region 260 is configured for inserting the sample solution tube 100, and after the sample solution tube 100 is inserted into the sample solution adding region, the accommodating chamber 210 is sealed, the puncturing mechanism 250 punctures the sample solution tube 100, and the sample solution flows into the accommodating chamber 210 from the fluid channel 251 to react with the chromatography test paper 240 in the accommodating chamber 210.
For example, the shape of the sample solution adding region 260 may be configured to match the sample solution tube 100, and after the sample solution tube 100 is inserted into the sample solution adding region 260, the surfaces of the two are attached to each other, so as to seal the accommodating cavity 210.
During the detection process, the accommodating cavity 210 is always sealed, so that the amplification product is prevented from leaking outwards. After the detection result is recorded, the destruction operation member 230 is moved to the second position by pulling the operation portion 231, the upper surface of the destruction liquid storage tank 220 is opened, and the chromatography test paper 240 can react with the nucleic acid destruction reagent to completely remove the nucleic acid remaining in the chromatography test paper detection apparatus. Therefore, even if the sample solution tube 100 is accidentally dropped or the chromatography test paper detection device is damaged in the subsequent process, the internal exposure is caused, and no pollution is caused.
By adopting the technical scheme, after the chromatographic test paper is detected, the operating part is pulled from the outside of the shell, the damage operating part moves to the second position, the upper surface of the damage liquid storage tank is opened, so that the damage liquid in the damage liquid storage tank reacts with the sample solution on the chromatographic test paper and in the sample solution pipe, and the residual nucleic acid in the chromatographic test paper detection device is completely removed. Therefore, even if the sample solution pipe is accidentally dropped in the subsequent process or the chromatography test paper detection device is damaged, the internal exposure is caused, and no pollution is caused.
It should be noted that the specific structure of the destruction operating element and the connection mode with the housing are not limited in the present invention, and the destruction operating element may be reasonably arranged according to the actual situation, as long as the destruction operating element can open the destruction liquid storage tank after the detection of the chromatography test paper is completed.
According to another embodiment of the present invention, as shown in fig. 1 to 5, the destruction operator is provided at one end of the housing 200, preferably, at an end of the housing 200 adjacent to the sample solution tube 100, the sealing part 232 of the destruction operator 230 is inserted into the housing 200 to seal the destruction liquid storage tank 220, the operating part 231 of the destruction operator 230 is inserted out of the housing 200 to facilitate manual operation, and the destruction operator 230 is sealingly connected to the housing 200 to prevent leakage of the destruction liquid from the destruction liquid storage tank 220, and the destruction operator 230 can be moved from the first position to the second position by operating the operating part 231.
Specifically, the end of the housing 200 is provided with a through hole 201, the through hole 201 is provided along the length direction (as shown by Y in fig. 2) of the housing 200, the destruction operating member 230 is installed in the through hole 201 along the length direction, and the upper surface of the destruction liquid storage tank 220 can be opened by pulling the destruction operating member 230. When the detection device is not used, the destructive liquid storage tank 220 is located at a first position, and at this position, the sealing part 232 of the destructive operation member 230 is broken to seal the destructive liquid storage tank 220, so that the liquid in the destructive liquid storage tank 220 does not react with the sample solution on the chromatographic test paper 240 and in the sample solution tube 100, and the chromatographic test paper 240 can be used smoothly. Pulling the operation part 231 of the destruction operation member 230 moves the destruction operation member 230 to the second position, and the upper surface of the destruction liquid storage tank 220 is opened, so that the destruction liquid in the destruction liquid storage tank 220 reacts with the sample solution on the chromatographic test paper 240 and in the sample solution tube 100, thereby preventing the nucleic acid remaining in the detection device from being contaminated when exposed inside.
In addition, in order to prevent the damage operation member 230 from being completely pulled out of the housing 200 and causing leakage of the liquid in the housing 200, referring to fig. 1 to 5, in the present embodiment, the bottom of the damage operation member 230 is provided with a limit protrusion 234, and the limit protrusion 234 can abut against the inner wall of the damage liquid storage tank 220 when the damage operation member 230 is excessively pulled out of the housing 100, thereby preventing the damage operation member 230 from being completely pulled out and ensuring that the liquid in the housing 200 does not leak.
Further, in order to ensure that the destruction operating member 230 can seal the destruction liquid storage tank 220 when the detection apparatus is not in use, and ensure the usability of the detection apparatus, according to another embodiment of the present invention, as shown in fig. 1 to 3, the detection apparatus further includes a locking device 254, the locking device 254 has a first state and a second state, the locking device 254 restricts the movement of the destruction operating member 230 from the first position to the second position when the locking device 254 is in the first state, and the locking device 254 releases the restriction on the movement of the destruction operating member 230 when the locking device 254 is in the second state. By providing the locking means 254, the operator is further prevented from operating erroneously, and the nucleic acid-disrupting reagent is brought into contact with the chromatographic test paper 240 when the detection has not been completed.
Specifically, referring to fig. X, in the present embodiment, the locking device 254 is disposed at a lower portion of the puncturing mechanism 250, the puncturing mechanism 250 can be in threaded engagement with the lower end of the sample solution tube 100, that is, the lower end of the sample solution tube 100 can extend into an upper inner cavity of the puncturing mechanism 250, the lower end of the puncturing mechanism 250 is provided with an external thread, the upper inner cavity of the puncturing mechanism 250 is provided with an internal thread, and a guiding structure is disposed between an outer wall of the puncturing mechanism 250 and an inner wall of the first channel. The sample solution tube 100 is rotated to enable the puncturing mechanism 250 to move upward by the screw thread and to be close to the lower end of the sample solution tube 100 and to puncture the bottom of the sample solution tube 100, and the sample solution flows into the receiving chamber 210 from the fluid passage 253 to react with the chromatography test paper 240 in the receiving chamber 210.
Wherein the guiding structure comprises a guiding protrusion 251 provided on the outer wall of the puncturing mechanism 250 and a guiding groove 261 provided on the inner wall of the first channel, the guiding protrusion 251 extends into the guiding groove 261 for limiting the axial movement (shown by X in fig. 2) of the puncturing mechanism 250 along the first channel 260, so that the puncturing part 252 of the puncturing mechanism 250 punctures the bottom of the product solution pipe 100.
The locking means 254 is disposed at a lower portion of the puncturing mechanism 250, and when the sample solution tube 100 does not drive the puncturing mechanism 250 to move upward, i.e., when the locking means 254 is in the first state, the locking means 254 restricts the movement of the destruction operating member 230 from the first position to the second position. Specifically, the destruction operating element 230 is provided with a locking hole 233, and the locking device 254 can be inserted into the locking hole 233 to restrict the movement of the destruction operating element 230, thereby preventing erroneous operation. When the puncturing mechanism 250 moves upward, the locking device 254 can be disengaged from the locking hole 233, that is, the locking device 254 is in the second state, after the sample solution reacts with the chromatographic test paper 240, the operation part 231 of the destruction operation member 230 is pulled to move the destruction operation member 230 to the second position, the upper surface of the destruction liquid storage tank 220 is opened, so that the destruction liquid in the destruction liquid storage tank 220 reacts with the sample solution on the chromatographic test paper 240 and in the sample solution tube 100, and the nucleic acid remaining in the detection device is prevented from being contaminated when exposed inside.
According to another embodiment of the present invention, a resilient pressing structure 280 is disposed above the chromatographic test strip 240, and when the baffle 230 is opened, the resilient pressing structure 280 presses at least a portion of the chromatographic test strip 240 into the destructive liquid storage tank 220. The elastic pressing structure 280 is not particularly limited, and may be a spring or a leaf spring.
Further, the specific structure for realizing the sealing of the accommodating chamber 210 may refer to any manner known in the prior art, which is not described herein, for example, the sample solution adding region 260 may be configured to match the sample solution tube 100 by setting the shape thereof, and after the sample solution tube 100 is inserted into the sample solution adding region 260, the surfaces of the two are attached to each other, so as to realize the sealing of the accommodating chamber 210. According to another embodiment of the present invention, the sample solution adding region 260 includes a cylindrical first channel (not shown), and in order to more effectively prevent the expansion product from leaking into the air, a sealing ring made of elastomer may be further provided at the insertion end of the first channel, or a sealing ring made of elastomer may be provided outside the sidewall 110 of the sample solution tube 100.
According to another embodiment of the present invention, the test result observation area 270 is made of a transparent material for easy observation.
It should be noted that, the specific structure and the manufacturing material of the sample solution tube are not limited in the present invention, and may be reasonably selected according to actual needs, as long as the sample solution tube is ensured to be capable of stably storing the sample solution and being punctured by the puncturing mechanism.
Specifically, referring to fig. 1-2, in the present embodiment, the sample solution tube 100 includes a side wall 110, a bottom wall 120 and an upper wall 130, and a sealed sample solution storage chamber 140 defined by the side wall 110, the bottom wall 120 and the upper wall 130, wherein the side wall 110 is a cylindrical side wall, and the outer surface of the side wall 110 is provided with external threads 150 for matching with the internal threads of the first channel 260 to achieve the sealing of the side wall 110 of the sample solution tube 100 with the first channel.
According to another embodiment of the present invention, in order to facilitate the sample solution tube 100 to be punctured by the puncturing mechanism 250 after being inserted into the chromatography test strip detecting apparatus, the bottom wall 120 of the sample solution tube 100 may be provided with a cut mark to facilitate the puncturing mechanism 250 to puncture the sample solution tube 100.
Further, since the bottom wall 120 of the sample solution tube 100 is provided with a cut, the sample solution tube 100 may be damaged at the cut before being inserted into the chromatographic strip detection device during the taking and placing process. Therefore, according to another embodiment of the present invention, the bottom wall 120 of the sample solution tube 100 is recessed into the sample solution storage cavity 140 to form the recess 121, and only the notch is formed in the recess 121, so that the damage of the sample solution tube 100 during the taking and placing process can be effectively avoided.
According to another embodiment of the present invention, the sample solution tube 100 is generally of an open-lid structure, i.e., the upper wall 130 is an openable lid, and the sample to be amplified and the reagents related to the amplification reaction system are put into the sample solution tube 100 and then the lid is closed to achieve sealing. In addition, the upper portion of the sample solution tube 100 may be directly of a closed structure, i.e., the upper wall 130 and the side wall 110 are fixedly connected, or even directly integrally formed, and cannot be opened. When in use, the injector with a thin needle penetrates through the upper wall 130 of the sample solution tube 100, the reaction system is injected, and then the opening is sealed by a sealing film or a wax drop with a higher melting point, so that the sample solution tube 100 can be better sealed.
Further, some poor or laggard areas have been heavily stricken by infectious diseases due to poor quality, poor hygiene, low hygiene awareness, malnutrition, etc. The infectious diseases have high incidence and death rate, and the high treatment cost is difficult to be borne by the ordinary families. However, in these areas, advanced infectious disease detection methods are not widespread, mainly because of the difficulty in supplying power in most areas and the inability to operate large instruments; the cost of large-scale medical equipment and the corresponding cost of maintenance equipment cannot be borne; site restriction; the patient cannot afford the high examination cost, etc. While the amplification reaction in the sample solution tube 100 needs to be performed in a specific temperature range, it may be difficult for the detection personnel to obtain a temperature constant device in the above-mentioned region, and the detection cannot be performed on the spot, which limits the instantaneity of the nucleic acid detection.
According to another embodiment of the present invention, the surface of the sample solution tube 100 is coated with at least two reversible temperature-sensitive color-changing materials. The color-changing temperature of the temperature-sensitive color-changing material can be set according to the actual situation, and the specific reversible temperature-sensitive color-changing material can be a commercially available product. For a certain amplification reaction, if the reaction temperature is required to be between a first temperature T1 and a second temperature T2, two temperature-sensitive color-changing materials can be selectively coated on the surface of the sample solution tube 100, wherein the color-changing temperature of the first temperature-sensitive color-changing material is the first temperature T1, and the color-changing temperature of the second temperature-sensitive color-changing material is the second temperature T2. Thus, when the first temperature sensitive material is discolored and the second temperature sensitive material is not discolored during the amplification reaction, it indicates that the temperature is suitable for the amplification reaction in the sample solution tube 100. In this way, the sample solution tube 100 can be directly placed in a thermos flask, and the temperature of water in the thermos flask can be controlled by adjusting the amount of cold and hot water, so that the amplification reaction can be maintained, and the sample can be detected at any time and any place without using a thermostat.
For example, if the optimal reaction temperature is about 38 ℃, the temperature-sensitive paint can be selected from 2 temperatures, which are respectively greater than 38 and less than 38, preferably 37 and 39 ℃, if the optimal reaction temperature is 63 ℃, the temperature-sensitive paint can be selected from 62 and 64, the shape of the temperature-sensitive paint can be any, but preferably arabic numerals corresponding to the temperature, such as 38-degree discolored temperature-sensitive material, shown as "38". This allows a more direct reaction of the temperature of the sample solution tube 100.
Further, if the sample solution tube 100 is used in amplification reaction systems with different temperatures, a plurality of temperature-sensitive color-changing materials can be arranged.
According to another embodiment of the present invention, the sample solution tube 100 of the present invention is a specially-made nucleic acid amplification tube, and the sidewall 110 of the sample solution tube 100 may be made of a series of materials, preferably a material having good thermal conductivity, high strength and good fluidity, such as metal, alloy, thermal conductive plastic and organic composite material, with a height of 1-3cm, preferably 2cm, and a general shape similar to that of a conventional nucleic acid amplification tube, but with some differences.
While the invention has been shown and described with reference to certain preferred embodiments thereof, it will be understood by those skilled in the art that the foregoing is a more detailed description of the invention, taken in conjunction with the specific embodiments thereof, and that no limitation of the invention is intended thereby. Various changes in form and detail, including simple deductions or substitutions, may be made by those skilled in the art without departing from the spirit and scope of the invention.
Claims (5)
1. A chromatography test paper detection device is characterized by comprising a shell, a destruction operating part, chromatography test paper, a puncture mechanism and a sample solution tube,
a containing cavity is arranged in the shell, a destructive liquid storage tank is arranged in the containing cavity, and the chromatography test paper is arranged above the destructive liquid storage tank and can react with the sample solution in the sample solution pipe;
the detection device comprises a shell, a damage operating part and a detection device, wherein the shell is provided with a through hole, the damage operating part is arranged on the shell in a penetrating mode through the through hole, the damage operating part comprises an operating part and a sealing part, the operating part is positioned outside the shell, the sealing part is positioned in the shell and seals the upper surface of the liquid storage tank, the damage operating part is provided with a first position and a second position, when the detection device is not used, the damage operating part is positioned in the first position, the damage operating part can be moved to the second position through the operating part, the liquid storage tank is opened, and the damage operating part is always in sealing connection with the shell;
the puncture mechanism is arranged in the shell and provided with a fluid channel, and the puncture mechanism can puncture the bottom wall of the sample solution tube, so that the sample solution flows into the containing cavity from the fluid channel to react with the chromatography test paper.
2. The chromatography strip detecting apparatus according to claim 1, wherein said detecting apparatus further comprises a locking device having a first state and a second state, said locking device restricting the movement of said destruction operating member from the first position to the second position when said locking device is in the first state, and said locking device releasing the restriction of the movement of said destruction operating member when said locking device is in the second state.
3. The chromatography test paper detection device of claim 1, wherein an elastic pressing structure is provided above the chromatography test paper, and when the liquid destruction operation member is opened, the elastic pressing structure presses at least part of the chromatography test paper into the liquid storage tank.
4. The chromatography test paper detection device of claim 3, wherein the upper surface of the housing is provided with a sample solution adding region, the sample solution adding region comprises a cylindrical first channel, an insertion end of the first channel is provided with a sealing ring made of an elastomer, or the side wall of the sample solution tube is externally provided with a sealing ring made of an elastomer.
5. The device of claim 3, wherein the liquid storage tank contains a sodium hypochlorite solution or a DNA detergent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010648508.8A CN111876320A (en) | 2020-07-07 | 2020-07-07 | Chromatography test paper detection device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010648508.8A CN111876320A (en) | 2020-07-07 | 2020-07-07 | Chromatography test paper detection device |
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| CN111876320A true CN111876320A (en) | 2020-11-03 |
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| CN202010648508.8A Pending CN111876320A (en) | 2020-07-07 | 2020-07-07 | Chromatography test paper detection device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022198956A1 (en) * | 2021-03-25 | 2022-09-29 | 江苏硕世生物科技股份有限公司 | Test paper testing auxiliary device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022198956A1 (en) * | 2021-03-25 | 2022-09-29 | 江苏硕世生物科技股份有限公司 | Test paper testing auxiliary device |
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