CN212476751U - Closed chromatography test paper card box - Google Patents
Closed chromatography test paper card box Download PDFInfo
- Publication number
- CN212476751U CN212476751U CN202021318826.XU CN202021318826U CN212476751U CN 212476751 U CN212476751 U CN 212476751U CN 202021318826 U CN202021318826 U CN 202021318826U CN 212476751 U CN212476751 U CN 212476751U
- Authority
- CN
- China
- Prior art keywords
- nucleic acid
- detection
- chromatography test
- test paper
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Abstract
The utility model discloses a closed chromatography test paper card box, include: the detection device comprises a shell, a detection cavity and a detection unit, wherein the detection cavity is formed in the shell; the chromatographic test paper is arranged in the detection cavity; a liquid feeding opening provided on the upper surface of the casing, communicating with the detection chamber, and configured to be inserted by the nucleic acid amplification tube and seal the detection chamber; the spine part is arranged in the liquid feeding opening; and the cleaning component stores a nucleic acid cleaning reagent and can inject the nucleic acid cleaning reagent into the detection cavity. By adopting the technical scheme, the detection cavity is kept sealed in the detection process, and the amplification product is prevented from leaking outwards. After the detection result is recorded, the nucleic acid cleaning reagent in the cleaning component can be injected into the detection cavity, and the amplification product can react with the nucleic acid cleaning reagent to completely remove the residual nucleic acid in the chromatography test paper cartridge. Therefore, even if the nucleic acid amplification tube is accidentally dropped in the subsequent process or the chromatography test paper cartridge is damaged to expose the inside, no pollution is caused.
Description
Technical Field
The utility model relates to a detection area, concretely relates to closed chromatography test paper card box.
Background
Nucleic acid diagnostics is one of the most active segments of the future IVD (in vitro diagnostics) industry. The increasing of the prevention and treatment of infectious diseases, the popularization of blood screening nucleic acid detection and the development of individualized medical treatment in China are the main motivations for the development of nucleic acid diagnosis in China. Under the promotion of the factors, the future speed increase of domestic nucleic acid diagnosis is 25-30%, and obviously exceeds the average speed increase of domestic IVD industry. On one hand, the nucleic acid diagnosis benefits large medical centers and realizes early, rapid, specific and high-throughput detection of pathogens, genetic diseases and the like.
POCT (Point-of-care testing), which is a new subdivision industry of In Vitro Diagnosis (IVD), is a new method for analyzing samples immediately on a sampling site, saving complex processing procedures of samples during laboratory testing and quickly obtaining testing results. The main criteria for POCT are that no fixed detection site is required, that the reagents and instruments are portable and that they can be operated in a timely manner. POCT plays the role of a laboratory, does not need traditional hospital laboratory equipment, and can serve patients in all directions within 24 hours without the limitation of time and place.
However, these nucleic acid amplification methods have a problem that the amplification products are easily cross-contaminated, and false positive signals generated by product contamination may cause erroneous interpretation of the detection results. Cross-contamination between samples is often seen during target nucleic acid amplification procedures, and contamination may result from known or unknown positive species introduced during negative sample processing, which causes false positive reactions through air contamination or aerosols. Therefore, nucleic acid detection needs to be performed in a special closed laboratory, and is difficult to perform in real time in some poor or laggard areas.
In the prior art, a series of methods have been developed to prevent cross contamination of amplification products, and for example, reference 1(CN105199940A) discloses a portable anti-contamination gene detection method and apparatus, by which a nucleic acid amplification tube containing an amplification product can be sealed in an apparatus and then the nucleic acid amplification tube is punctured to perform detection. Prevent the pollution of nucleic acid amplification products and avoid false positive. However, in this apparatus, since the nucleic acid amplification tube is sealed after being placed in the apparatus, the puncturing operation is difficult, and the amplification product after the completion of the test remains in the apparatus, and if the nucleic acid amplification tube is broken, the amplification product may also diffuse into the air, causing a false positive reaction.
Further, while reference 2(CN203241416U) discloses a closed type chromatography strip plastic cartridge, and reference 3(CN205574438U) discloses a sealed tube assembly including a tube breaking mechanism, these detection devices are convenient to operate, but the amplification product remains in the device after detection, which may cause contamination.
Therefore, it is an urgent problem in the art to provide a nucleic acid detecting apparatus with less contamination possibility before and after detection.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the closed detection device among the prior art and operate inconveniently, and still have the problem of pollution possibility after detecting. In order to solve the problem, the utility model discloses a closed chromatography test paper card box of brand-new structure, through this chromatography test paper card box convenient operation to can effectually avoid the amplification product to spread to the air in, cause false positive reaction.
In order to solve the problem, the utility model discloses a closed chromatography test paper card box, include:
the detection device comprises a shell, a detection cavity and a detection unit, wherein the detection cavity is formed in the shell;
the chromatographic test paper is arranged in the detection cavity;
a liquid feeding opening provided on the upper surface of the casing, communicating with the detection chamber, and configured to be inserted by the nucleic acid amplification tube and seal the detection chamber;
a sharp part arranged in the liquid feeding opening and used for piercing the bottom of the inserted nucleic acid amplification tube;
and the cleaning component stores a nucleic acid cleaning reagent and can inject the nucleic acid cleaning reagent into the detection cavity.
By adopting the technical scheme, the detection cavity is kept sealed in the detection process, and the amplification product is prevented from leaking outwards. Further, after the detection result is recorded, the nucleic acid cleaning reagent in the cleaning component can be injected into the detection cavity, and the amplification product can react with the nucleic acid cleaning reagent to completely remove the residual nucleic acid in the chromatography test paper cartridge. Therefore, even if the nucleic acid amplification tube is accidentally dropped in the subsequent process or the chromatography test paper cartridge is damaged to expose the inside, no pollution is caused.
According to another embodiment of the present invention, the cleaning assembly is disposed at an end of the housing along a first direction, the first direction being a direction in which the chromatography test paper extends.
According to another embodiment of the present invention, the cleaning assembly comprises a movable stopper rod and a cleaning chamber,
a nucleic acid cleaning reagent is stored in the cleaning cavity and is communicated with the detection cavity through a liquid injection port, and the liquid injection port is sealed by a liquid blocking piece;
the movable plunger rod comprises a piston part and a support rod part, the piston part is arranged in the cleaning cavity and is far away from one end of the liquid injection port, at least part of the support rod part is positioned outside the shell, the piston part can move towards the direction of the liquid injection port in the cleaning cavity by operating the support rod part, the nucleic acid cleaning reagent is compressed, the resistance liquid part is broken, and the nucleic acid cleaning reagent in the cleaning cavity is injected into the detection cavity.
According to the utility model discloses a another embodiment still includes the protective housing, and the protective housing cover is established outside the vaulting pole portion to be connected with shell detachable.
According to another embodiment of the present invention, the protective housing is connected to the housing by a snap fit.
According to another embodiment of the present invention, a flow guiding through hole is provided in the spine portion.
According to another embodiment of the present invention, the device further comprises a nucleic acid amplification tube inserted into the liquid feeding opening to seal the detection chamber.
According to another embodiment of the present invention, the nucleic acid amplification tube and the liquid addition opening are connected by a snap fit or a screw thread.
According to another embodiment of the present invention, an annular sealing gasket is further provided in the liquid feeding opening.
Drawings
The invention will be described in further detail with reference to the following drawings and detailed description:
fig. 1 is a schematic structural diagram of a closed chromatography test paper cartridge provided by the present invention;
FIG. 2 is a schematic cross-sectional view of the enclosed chromatography test paper cartridge according to the present invention in one state;
FIG. 3 is a schematic cross-sectional view of the enclosed chromatography test strip cartridge according to the present invention in still another state;
fig. 4 is an explosion diagram of the closed chromatography test paper cartridge provided by the present invention.
Reference numerals:
nucleic acid amplification tube 100
Moving plug 222
Piston portion 2222
Spike 250
Filling opening 260
Test result observation area 270
Detailed Description
The following description is provided for illustrative embodiments of the present invention, and other advantages and effects of the present invention will be readily apparent to those skilled in the art from the disclosure herein. While the invention will be described in conjunction with the preferred embodiments, it is not intended that features of the invention be limited to only those embodiments. On the contrary, the intention of implementing the novel features described in connection with the embodiments is to cover other alternatives or modifications which may be extended based on the claims of the present invention. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. The invention may be practiced without these particulars. Furthermore, some of the specific details are omitted from the description so as not to obscure or obscure the present invention. It should be noted that, in the present invention, the embodiments and features of the embodiments may be combined with each other without conflict.
In the description of the present embodiment, it should be noted that the terms "upper", "lower", "inner", "bottom", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings or orientations or positional relationships that the products of the present invention are usually placed in when used, and are only for convenience of describing the present invention and simplifying the description, but do not indicate or imply that the device or the element to which the present invention is directed must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present invention.
The terms "first," "second," and the like are used solely to distinguish one from another and are not to be construed as indicating or implying relative importance.
In the description of the present embodiment, it should be further noted that, unless explicitly stated or limited otherwise, the terms "disposed," "connected," and "connected" are to be interpreted broadly, e.g., as a fixed connection, a detachable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present embodiment can be understood in specific cases by those of ordinary skill in the art.
As shown in fig. 1-4, the utility model discloses a closed chromatography test paper card box, include:
a housing 200, a detection cavity 210 is formed in the housing 200;
the chromatographic test paper 240, the chromatographic test paper 240 is arranged in the detection cavity 210;
an addition opening 260 provided on the upper surface of the housing 200, communicating with the detection chamber 210, and configured to be inserted by the nucleic acid amplification tube 100 and to seal the detection chamber 210;
a sharp part 250 provided in the filling opening 260 for piercing the bottom of the inserted nucleic acid amplification tube 100;
a cleaning component storing a nucleic acid cleaning reagent and capable of injecting the nucleic acid cleaning reagent into the detection chamber 210.
By adopting the technical scheme, the detection cavity 210 keeps sealed in the detection process, and the amplification product is prevented from leaking outwards. Further, after recording the detection result, a nucleic acid cleaning reagent in the cleaning assembly may be injected into the detection chamber 210, and the amplification product may react with the nucleic acid cleaning reagent to completely remove the nucleic acid remaining in the cartridge of the chromatography test paper 240. Thus, even if the nucleic acid amplification tube 100 is inadvertently detached or the cartridge of the chromatographic test paper 240 is damaged to expose the inside thereof in a subsequent process, no contamination is caused.
According to another embodiment of the present invention, the cleaning assembly is disposed at one end of the housing 200 along a first direction, which is the direction in which the chromatographic test paper 240 extends (i.e., the X-X direction shown in fig. 2).
In accordance with another embodiment of the present invention, the cleaning assembly includes a movable plunger 222 and a cleaning chamber 221,
the cleaning cavity 221 is stored with nucleic acid cleaning reagent and is communicated with the detection cavity 210 through a liquid injection port 223, and the liquid injection port 223 is sealed by a liquid blocking member;
the movable plunger 222 includes a piston portion 2222 and a lever portion 2221, the piston portion 2222 is disposed at one end of the cleaning chamber 221 away from the injection port 223, the lever portion 2221 is at least partially located outside the housing 200, and the piston portion 2222 can be moved in the cleaning chamber 221 toward the direction of the chromatographic test paper 240 by operating the lever portion 2221, so as to compress the nucleic acid cleaning reagent, break the blocking liquid member, and inject the nucleic acid cleaning reagent in the cleaning chamber 221 into the detection chamber 210.
Specifically, when the detection is not completed, the movable plug 222 is located at the first position shown in fig. 2, and the liquid blocking member is sealed at the liquid inlet 223. The nucleic acid amplification tube 100 can be inserted into the liquid adding opening 260, the detection cavity 210 is sealed, the sharp part 250 punctures the nucleic acid amplification tube 100, the nucleic acid solution to be detected is dropped onto the chromatographic test paper 240, and the detection result can be observed later.
After the detection is completed, the plunger 222 can be conveniently pushed and moved by the support portion 2221 located outside the housing 200, and moved to the position shown in fig. 3 along the first direction toward the position of the liquid injection port 223, and the nucleic acid cleaning reagent is compressed to break the liquid blocking member, and flows into the detection chamber 210 from the liquid injection port 223, so that the nucleic acid in the detection chamber 210 is completely destroyed, and the operation is convenient and fast.
Further, the structure of the liquid blocking member is not particularly limited, and an elastic rubber plug disposed in the liquid injection port 223 may be an elastic rubber plug, or a plastic film, a sealing film, or a wax seal adhered to the outside of the liquid injection port 223.
According to another embodiment of the present invention, in order to avoid mistakenly touching the strut portion 2221 before the detection is completed, and thus make the nucleic acid cleaning reagent destroy the chromatography test paper 240, the closed chromatography test paper cartridge further comprises a protective housing 230, the protective housing 230 is sleeved on the strut portion 2221 and detachably connected with the housing 200, preferably, the protective housing 230 is connected with the housing 200 in a snap-fit manner.
According to another embodiment of the present invention, a flow guiding through hole is disposed in the spine portion 250, the spine portion 250 can be a needle of a syringe, the nucleic acid amplification tube 100 is pierced along with the insertion of the nucleic acid amplification tube 100, and the nucleic acid liquid to be detected falls onto the chromatographic test paper 240 along the flow guiding through hole in the spine portion 250.
According to another embodiment of the present invention, the device further comprises a nucleic acid amplification tube 100, wherein the nucleic acid amplification tube 100 is inserted into the liquid feeding opening 260 to seal the detection chamber 210. Realize detecting the sealed concrete structure in chamber 210 and can refer to any mode that has in the prior art, the utility model discloses in not giving unnecessary details, for example liquid feeding opening 260 can be through setting up the shape with nucleic acid amplification pipe 100 phase-match, after nucleic acid amplification pipe 100 inserts liquid feeding opening 260, the two surface is laminated mutually, realizes detecting the sealed of chamber 210.
Further, in order to secure stable connection of the nucleic acid amplification tube 100 and the liquid addition opening 260, it is preferable that the nucleic acid amplification tube 100 and the liquid addition opening 260 are connected by a snap connection or a screw connection.
In order to further ensure the sealing connection between the nucleic acid amplification tube 100 and the filling opening 260, an annular sealing gasket (not shown in the middle) is further provided in the filling opening 260.
According to another embodiment of the present invention, the housing 200 is further provided with a detection result observation area 270 made of a transparent material.
According to another embodiment of the present invention, the nucleic acid cleaning reagent may be a sodium hypochlorite solution or a commercial DNA detergent or the like.
In summary, according to the technical scheme of the present application, in the detection process, the detection cavity 210 remains sealed, so that the amplification product is prevented from leaking outwards. Furthermore, after the detection result is recorded, the nucleic acid cleaning reagent in the cleaning component can be injected into the detection chamber 210, and the amplification product can react with the nucleic acid cleaning reagent to completely remove the nucleic acid remaining in the cartridge of the chromatographic test paper 240, so that the operation is convenient and fast. Thus, even if the nucleic acid amplification tube 100 is inadvertently detached or the cartridge of the chromatographic test paper 240 is damaged to expose the inside thereof in a subsequent process, no contamination is caused.
While the invention has been shown and described with reference to certain preferred embodiments thereof, it will be understood by those skilled in the art that the foregoing is a more detailed description of the invention, and the specific embodiments thereof are not to be considered as limiting. Various changes in form and detail, including simple deductions or substitutions, may be made by those skilled in the art without departing from the spirit and scope of the invention.
Claims (9)
1. An enclosed chromatography test strip cartridge, comprising:
the detection device comprises a shell, a detection cavity and a detection sensor, wherein the detection cavity is formed in the shell;
the chromatographic test paper is arranged in the detection cavity;
a liquid adding opening provided on the upper surface of the housing, communicating with the detection chamber, and configured to be inserted by a nucleic acid amplification tube and seal the detection chamber;
a sharp part arranged in the liquid adding opening and used for piercing the bottom of the inserted nucleic acid amplification tube;
a cleaning component storing a nucleic acid cleaning reagent and capable of injecting the nucleic acid cleaning reagent into the detection chamber.
2. The closed chromatography test strip cartridge of claim 1, wherein the cleaning member is disposed at one end of the housing in a first direction, the first direction being a direction in which the chromatography test strips extend.
3. The closed chromatography test strip cartridge of claim 2, wherein the cleaning assembly comprises a movable plug and a cleaning chamber,
the nucleic acid cleaning reagent is stored in the cleaning cavity and is communicated with the detection cavity through a liquid injection port, and the liquid injection port is sealed by a liquid blocking piece;
remove the cock stem and include piston portion and vaulting pole portion, the piston portion sets up clean intracavity is kept away from the one end of annotating the liquid mouth, the vaulting pole portion part at least is located outside the shell, through the operation the vaulting pole portion can make the piston portion be in clean intracavity removes towards the direction of annotating the liquid mouth place, compresses the clean reagent of nucleic acid, breaks liquid blocking piece, will clean intracavity the clean reagent of nucleic acid is injected into detect the intracavity.
4. The closed chromatography test paper cartridge of claim 3, further comprising a protective shell, wherein the protective shell is sleeved outside the strut portion and detachably connected with the shell.
5. The closed chromatography test strip cartridge of claim 4, wherein the protective shell is snap-fit to the housing.
6. The closed chromatography test paper cartridge of claim 1, wherein the spine portion is provided with a flow guide through hole.
7. The closed-type chromatography test paper cartridge of claim 1, further comprising a nucleic acid amplification tube inserted into the liquid addition opening to seal the detection chamber.
8. The closed-type chromatography test paper cartridge of claim 1, wherein the nucleic acid amplification tube and the liquid addition opening are connected by a snap-fit connection or a screw-thread connection.
9. The closed chromatography test paper cartridge of claim 1, wherein an annular sealing gasket is further provided in the liquid feeding opening.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021318826.XU CN212476751U (en) | 2020-07-07 | 2020-07-07 | Closed chromatography test paper card box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021318826.XU CN212476751U (en) | 2020-07-07 | 2020-07-07 | Closed chromatography test paper card box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN212476751U true CN212476751U (en) | 2021-02-05 |
Family
ID=74456157
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202021318826.XU Active CN212476751U (en) | 2020-07-07 | 2020-07-07 | Closed chromatography test paper card box |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN212476751U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113484514A (en) * | 2021-03-25 | 2021-10-08 | 江苏硕世生物科技股份有限公司 | Test paper detection auxiliary device |
-
2020
- 2020-07-07 CN CN202021318826.XU patent/CN212476751U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113484514A (en) * | 2021-03-25 | 2021-10-08 | 江苏硕世生物科技股份有限公司 | Test paper detection auxiliary device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111876318A (en) | Closed chromatography test paper card box | |
| EP1756565B1 (en) | Specimen collecting, processing and analytical assembly | |
| US20090305315A1 (en) | Hubbed dual cannula device for closed container sampling systems | |
| CN101278183A (en) | Apparatus and method for detecting an analyte | |
| JP2006308428A (en) | Nucleic acid analysis method, nucleic acid analyzer, and cartridge for nucleic acid analysis | |
| JP4650102B2 (en) | Sample collection liquid container | |
| CN104135927A (en) | Blood-sampling tube with integrated sensor device | |
| CN212476751U (en) | Closed chromatography test paper card box | |
| CN116242994B (en) | Household blood routine automatic analyzer | |
| CN111876319A (en) | Reaction tube and test kit | |
| CN111876304A (en) | Test paper detection box | |
| CN216192304U (en) | Detection device for nucleic acid amplification product | |
| JP2009216651A (en) | Multi-item detector | |
| CN104818213A (en) | Sliding type closed pathogen detection card box | |
| CN111925907A (en) | Combined nucleic acid detection device | |
| CN111982894A (en) | Portable test paper detection device | |
| CN212476749U (en) | Chromatography test paper detection device | |
| CN111876320A (en) | Chromatography test paper detection device | |
| CN212476727U (en) | Sample solution tube and detection device | |
| WO2022213383A1 (en) | Closed nucleic acid test consumable and test method | |
| CN212476703U (en) | Combined nucleic acid detection device | |
| CN212476726U (en) | Gene amplification tube and detection card box | |
| CN212476750U (en) | Reaction tube and test kit | |
| CN212476748U (en) | Nucleic acid amplification detection device | |
| CN212483361U (en) | Portable test paper detection device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |