JP2009216651A - Multi-item detector - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a multi-item detector capable of performing all detections in a closed system. <P>SOLUTION: The multi-item detector includes a first sealable container capable of sealing a specimen process liquid in and a second sealable container for housing the first container and detects one item to be detected or more on a substance to be detected by simultaneously applying one immunochromatography carrier or more to a specimen extract liquid acquired by injecting a specimen to the specimen process liquid and extracting the substance to be detected. The first container is provided with a specimen collection rod and the specimen process liquid. The second container is provided with one immunochromatography carrier or more for detecting the items to be detected by specific reaction with the substrate to be detected in the specimen extract liquid when the specimen extract liquid has permeated and individually capable of detecting different types of items to be detected different from one another. The multi-item detector is provided with a leakage means for specimen extract liquids and for braking the back of the first container and making the specimen extract liquid leak out when the first container is housed in the second container. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to a multi-item detection apparatus, and more particularly, to an analyte chromatographic liquid (a carrier for immunochromatography) of one or more test items with respect to the test substance in a closed system with respect to a sample extract from which the test substance is extracted. The present invention relates to a multi-item detection apparatus that performs detection by a single detection apparatus.

For specimens such as blood, urine, saliva, nasal mucus, stool samples, etc., if there are many items to be detected with respect to the test substance, that is, if there are many test items, the specimen extract in which the specimen sample is dissolved or suspended is usually used. It puts into many detection apparatuses (or test containers, such as a test tube and a test cell), and performs different analysis operation according to the item which should be detected about each of these detection apparatuses.
When such a detection method is used, it is necessary to prepare a large number of detection devices as the number of items to be detected increases, and it is necessary to perform analysis operations individually by replacing a large number of detection devices. It takes time and the analysis operation becomes complicated.
In addition, infectious or potentially infectious specimens, or specimens that are very important in terms of ensuring public health, such as those infected with infectious diseases such as legal infectious diseases, influenza, AIDS, etc. When handling specimens, specimens that contain or possibly contain toxic substances such as PCBs, carcinogens, and trace amounts of substances, the detection device is opened to the surrounding environment during the analysis operation. If the analysis operation is performed in a closed state (for example, with the lid opened; open system), there is a risk of infection to the person who performs the analysis operation (worker), and conversely, the worker and / or work Incorrect analysis results may be obtained due to environmental contamination of the sample.
Further, when a large number of used detection devices are discarded, a serious problem can occur in terms of ensuring safety.
Therefore, when detecting many test items for one specimen, use as few detection devices as possible, if possible, one detection device, and seal all analysis operations after preparing the sample. It is preferable to carry out in a system.

One of many analytical means for a sample is an immunoassay. Conventionally, various methods and / or devices for immunoassays have been proposed. Among them, typical ones will be described below.
Japanese Patent Laid-Open No. 6-504846 (Patent Document 1) describes a mode in which detection of a test item related to a test substance can be easily performed without requiring a device for detection including temperature control. A detection method using a piece of immunochromatographic carrier has been proposed. Japanese Patent Application Laid-Open No. 9-501494 (Patent Document 2) discloses a technique in which a strip-shaped immunochromatographic carrier (matrix) is applied to a specimen after pretreatment. Furthermore, Japanese Patent Application Laid-Open No. 8-946184 (Patent Document 3) proposes a method of detecting two or more kinds of test substances with one immunochromatographic carrier (strip-shaped test piece). On the other hand, Japanese Patent Application Laid-Open No. 2006-194688 (Patent Document 4) proposes an immunochromatography kit in which a strip-shaped immunochromatographic carrier is inserted into a tubular test container (test tubular container) having an open end. Yes.

Analytical means using a device for a specimen has also been proposed. For example, Japanese Patent Publication No. 9-503581 (Patent Document 5) discloses a disposable electronic assay device, a sample receptor means for receiving a sample of a body fluid containing an analyte to be determined, and a sample body fluid component A sample processing means that reacts with the amount of analyte in the sample and produces a change that can be physically detected, and in response to the change that can be physically detected, Detector means for generating an electrical signal correlating with the amount of analyte, signal processing means connected to the detector means for converting the electrical signal into a digital test result output, connected to the signal processing means, and An apparatus is disclosed comprising a card-type housing comprising visually readable output means for receiving and presenting test result signals.
Japanese National Patent Publication No. 6-504846 JP-T 9-501494 JP-A-8-946184 JP 2006-194688 A JP-T 9-503581

When using the immunochromatographic carrier described in Patent Documents 1 to 4, detection operations and determination and display of analysis results are performed manually by a human. When the immunochromatographic carrier described in Patent Document 3 is used, two or more kinds of test substances can be detected by one immunochromatographic carrier, and the immunochromatographic carriers described in Patent Documents 1, 2, and 4 are also two kinds. It can be used to detect the above test substances.
However, the immunochromatographic carriers described in Patent Documents 1 to 3 are not used in a sealed state in a container. The immunochromatographic carrier described in Patent Document 4 also uses a test tubular container having one end opened. Therefore, when these immunochromatographic carriers are used, sample collection and analysis cannot be performed in a completely closed system.
When the electronic test device described in Patent Document 5 is used, detection operation and determination and display of analysis results are automatically performed. When the electronic test device described in Patent Document 5 is used, two or more types of test items, for example, light transmittance, reflectance, pH, and the like can be detected.
However, the sample receptor of the electronic assay device described in Patent Document 5 is open to the atmosphere, and sample reception by the sample receptor and subsequent analysis are performed in an open system.
All of the techniques described in Patent Documents 1 to 5 are open-system techniques, and it is impossible to avoid an error in analysis results due to sample contamination and sample contamination and sample contamination. . More specifically, it is impossible or very difficult to prevent workers from suffering from poisoning or poisoning due to harmful samples (for example, infectious pathogens, toxic substances, etc.) in the specimen, and the workers themselves When a substance releases a trace amount of a substance to be detected (test substance) (for example, when a trace quantity of a substance contained in the human body is detected), the analysis result is judged by the test substance emitted by the worker. May be mistaken (for example, it is originally negative but it is determined to be positive).
Conventionally, there has not been known a multi-item detection device that can detect a large number of test items related to a test substance in a specimen in a closed system using a single detection device. Further, there has not been known a multi-item analyzer that uses this multi-item detector and that can automatically perform detection operation, determination and display of analysis results, and the like using an electronic device.
The present inventors can perform a detection reaction after collecting a sample in a sample collection container and perform all the steps from detection of a large number of test items related to the test substance in the sample in a closed system. As a result of earnest research on a multi-item detection apparatus and a multi-item analysis apparatus using the multi-item detection apparatus, the present invention has been completed.

A first object of the present invention includes a sealable first container that can enclose a sample processing solution and a sealable second container that houses the first container, and the sample is charged into the sample processing solution. A multi-item detection apparatus that detects one or more test items with respect to a test substance by simultaneously applying one or more immunochromatographic carriers to the sample extract from which the test substance is extracted,
The first container comprises a sample collection rod and a sample processing solution,
The second container is an immunochromatographic carrier that detects the test item by specifically reacting with a test substance in the sample extract when the sample extract permeates, and the individual immunochromatographic carriers are different from each other. One or more immunochromatographic carriers capable of detecting the type of test item are provided, and when the first container is stored in the second container, the back of the first container is broken and the specimen extract leaks. A multi-item detection apparatus comprising a specimen extract leakage means for exiting.
The second subject of the present invention is the means for leaking the specimen extract,
A notch is provided in the back of the first container, and a protrusion for pressing and breaking the back of the first container on the inner back surface of the second container, and the first container in the second container When storing, the lid of the second container is closed so that the second container is sealed and the back of the first container is torn and the specimen extract leaks out. In the first item multi-item detection device, a convex portion for pressing the upper surface of the lid of the first container is provided on the lower surface of the first container.
The third object of the present invention is a multi-item detection apparatus of the first object, wherein the one or more immunochromatographic carriers are arranged in the circumferential direction on the inner peripheral surface of the second container. A detection device;
A fixed / rotating means for rotating the attached multi-item detection device so that data relating to a test item can be read from one or more immunochromatographic carriers,
Alignment means for confirming that the one or more immunochromatographic carriers have reached a position where the data can be read;
A sensor that reads the data from the one or more immunochromatographic carriers;
Control means for repeating the pause and restart of the fixing / rotating means so that the data is sequentially read from the one or more immunochromatographic carriers;
A multi-item analyzer comprising recording means for recording data obtained from the sensor.
A fourth object of the present invention is a multi-item analysis apparatus as a third object, wherein the sensor is a charge coupled device (CCD) image sensor and the alignment means is a bar code reader.
The fifth object of the present invention is the multi-item analyzer of the third object characterized in that the fixing / rotating means comprises a turntable and a motor.

When the multi-item detection apparatus of the present invention is used, all the steps from collecting a sample in a sample collection container to performing a detection reaction and detecting a large number of test items related to the test substance in the sample are made into a closed system. Analyzing operations can be performed on one or more items, particularly a large number of test items, while ensuring the safety of the worker for samples that are harmful or potentially harmful.
In the multi-item detection apparatus of the present invention, it is possible to prevent a substance that affects the analysis result from entering the sample from the operator during the analysis work, so that the reliability of the analysis result is improved.
In addition, since the number of detection devices necessary for detecting a large number of test items can be reduced to a minimum of one, it is easy to discard used detection devices.
Furthermore, if the multi-item analyzer of the present invention capable of automatically reading, recording and analyzing detection data from the multi-item detector of the present invention is used, the work efficiency in the analysis operation is improved.

The first container only needs to be a sealable container that can enclose the sample processing solution, and the material, shape, and size thereof can be selected as appropriate.
The second container only needs to be a sealable container that houses the first container, and the material, shape, and size thereof can be selected as appropriate.
The first container and the second container can be placed vertically or horizontally. When the first container and the second container are vertically placed containers, “back” means “bottom”. In the case where the first container and the second container are horizontal containers, “back” means, for the first container, the side end that breaks in order to leak the sample extract, and the second container Means a side end provided with a projection or the like for breaking the side end surface of the first container.
As the material of the first container or the second container, for example, transparent or translucent plastics such as polyethylene, polypropylene, polystyrene and the like can be used alone or in combination. The shape may be, for example, a cylindrical shape, a rectangular tube shape, a combination thereof, or the like. The size needs to be a size that allows the first container to be stored in the second container.
As the sample collection rod and the sample treatment liquid, those suitable for the sample to be analyzed are selected. The material, shape, and size of the sample collection rod, and the composition and volume of the sample processing solution are appropriately selected. The sample collection rod may be, for example, a cotton swab provided with a sample collection cotton ball.
At least one immunochromatographic carrier is arranged in the second container so as to be positioned in the gap between the first container and the second container when the first container is housed in the second container. The size and shape of the immunochromatographic carrier are appropriately selected so that they can be stored in the second container in a sealed state. The material of the immunochromatographic carrier needs to be a material that can be penetrated by the specimen extract. For example, materials commonly used in this field such as cellulose and glass fiber can be used alone or in combination.
The immunochromatographic carrier is impregnated or supported with a reagent that can specifically react with a test substance in the sample extract when permeated, or a detection portion impregnated or supported with the reagent is provided. . The detection portion may be attached in advance. In addition to the detection portion, for example, a pretreatment portion for pretreatment prior to detection, such as a filtration portion for filtering solid content, can be provided as appropriate.
For the immunochromatographic carrier, a casing may be used in order to protect the surface from damage and dirt, prevent deformation, etc., and enable stable analysis. Specifically, at least one surface of the immunochromatographic carrier, for example, both opposing surfaces are sandwiched by a transparent or opaque polymer sheet or the like, or the immunochromatographic carrier is placed in a polymer case (for example, a rectangular or cylindrical transparent polymer case). It may be stored.
One or more immunochromatographic carriers can be separated from the outside so that detection results can be read from the outside, and in the case of two or more immunochromatographic carriers, they do not come into contact with each other (ie, do not affect each other's detection results). For example, it is preferable to arrange in the gap in a state of being arranged in the circumferential direction on the inner peripheral surface of the second container. In this case, by using a partition member having a suitable size and shape, two or more immunochromatographic carriers can be arranged at a suitable distance from each other. The type of immunochromatographic carrier is selected according to the test item to be detected for the specimen.
In one or more immunochromatographic carriers, when there are two or more immunochromatographic carriers, the individual immunochromatographic carriers can detect different types of test items.
Further, the immunochromatographic carrier may be one capable of detecting one item with one immunochromatographic carrier, or may be capable of detecting many items of two or more items with one immunochromatographic carrier. . For example, as an immunochromatographic carrier, two or more detection units are provided on one immunochromatographic carrier as described in JP-A-2002-286716, JP-A-2004-184295, JP-T-2007-506115, and the like. It is also possible to use a single immunochromatographic carrier that can detect a large number of items.
The first container is sealable and may be provided with a detachable lid that can seal the first container by fitting or screwing, for example. The second container is also sealable like the first container, and may include a detachable lid that can seal the second container by fitting or screwing, for example.

The specimen extract leakage means is not particularly limited as long as it is a means for breaking the back of the first container and leaking the specimen extract when the first container is stored in the second container.
For example, the first container may be provided with a back that can be broken or at least partially broken when pressed by a needle-like, conical, pyramid-like projection. The inner part is preferably formed with a thin wall or provided with a cut so as to be easily broken or broken. The second container may include an inner back surface provided with a protrusion for pressing and breaking the back of the first container. The protrusion may be formed integrally with the second container, or a separately formed protrusion may be disposed or stuck on the inner back surface of the second container. The number of protrusions may be one, but may be two or more.
The notch provided in the back of the first container may be provided on the one or both sides of the back as long as the back is easily broken or easily broken. For example, a large number of slits can be provided radially from the back center, and the width and depth are appropriately selected.
A convex part for pressing the lid of the first container is provided on the opposite side of the lid of the second container, and when the size and shape of the convex part are appropriately selected, the second container is closed by closing the lid of the second container. At the same time as or after sealing, it is possible to press the back of the first container with a convex portion and break it.

The multi-item detection device of the present invention, comprising the multi-item detection device in which the one or more immunochromatographic carriers are arranged in the circumferential direction on the inner peripheral surface of the second container. In the analyzer, data from each immunochromatographic carrier is sequentially read and recorded. As a control means in the multi-item analysis apparatus of the present invention, for example, when a personal computer equipped with a display and a printing machine is used, it is possible to appropriately repeat the temporary stop and restart of the fixing / rotating means, It is possible to configure a multi-item analyzer that automatically displays, records and analyzes the read data and displays, records and prints the analysis results. The recording means in the multi-item analyzer of the present invention may be a commonly used recording means such as a hard disk, a USB memory, or an MO. The multi-item analyzer of the present invention may be used by connecting to a network.
A charge coupled device (CCD) image sensor can be used as the sensor, and a bar code reader can be used as the alignment means. The barcode may be provided at a predetermined position of each immunochromatographic carrier, for example, and by this barcode, the type of each immunochromatographic carrier can be confirmed, and it can be confirmed that it has reached a position where data can be read. .
The fixing / rotating means may be composed of, for example, a turntable and a motor, and the size and shape of the turntable and motor are appropriately selected according to the size and shape of the multi-item detection device of the present invention.

In the following examples, the present invention will be described specifically and in more detail. The following examples are for illustrative purposes only and are not intended to limit the technical scope of the present invention.
Example 1:
FIG. 1 is an exploded perspective view of a vertical multi-item detection apparatus according to Embodiment 1 of the present invention. A sample processing solution 15 is contained in the first container 14 shown in FIG. The first container 14 has a thread groove (not shown) cut at the upper end, and can be sealed by screwing a lid 16 having a screw groove (not shown) cut inside. FIG. 1B shows a second container 17 for housing the first container 14. A large number of partition members 18 are provided inside the second container 17, and one or more immunochromatographic carriers 19 are arranged in the gaps between the two partition members 18 so as not to contact each other. (Only one immunochromatographic carrier 19 is shown). The immunochromatographic carrier 19 may use a casing (for example, sandwiching both opposing surfaces with a transparent or opaque polymer sheet or the like). A space in which the first container 14 can be stored is provided in the center of the second container 17. The second container 17 has a thread groove (not shown) cut at the upper end, and can be sealed by screwing the lid 20 having a screw groove (not shown) cut inside. A convex portion 22 is provided on the lower surface 21 of the lid 20. In addition, a projection 24 (for example, a triangular pyramid shape or a quadrangular pyramid shape) is provided on the inner back surface 23 of the second container 17. FIG.1 (c) is the cotton swab 26 provided with the cotton ball 25 for extract | collecting a test substance.

Hereinafter, a method for using the multi-item detection apparatus of FIG. 1 will be described.
(1) Samples such as nasal mucus and diarrheal stool are collected with a cotton ball 25 of a cotton swab 26.
(2) The cotton swab 26 is placed in the first container 14 so that the cotton ball 25 enters the sample processing solution 15, the lid 16 is closed, and then the first container 14 is shaken and stirred.
(3) The first container 14 is accommodated in the central space in the second container 17 in which one or more immunochromatographic carriers 19 are arranged, and the lid 20 is closed. At the same time as or after the second container 17 is sealed by the lid 20, the upper surface 27 of the lid 16 of the first container 14 is pressed by the convex portion 22 provided on the lower surface 21 of the lid 20. Then, the back 28 of the first container 14 is pressed and destroyed by the protrusion 24 provided on the inner back surface 23 of the second container 17, or at least a part thereof is broken. .
(4) The specimen extract leaked from the first container 14 (the test substance is extracted into the specimen treatment liquid 15) penetrates into the immunochromatographic carrier 19 from the lower end, and is mutually determined in advance in the individual immunochromatographic carrier 19 A specific reaction (detection reaction) with a different kind of test substance is started.
(5) After leaving as it is for a predetermined time (for example, several minutes), the analysis results of one or more immunochromatographic carriers 19 are sequentially read and judged in order. As will be described later, it is also possible to put the second container 17 in the state (4) into a dedicated chromatographic reader and automatically read and determine the analysis results for a number of immunochromatographic carriers 19 in order.
(6) The second container 14 is discarded as it is.
Since all the steps (2) to (5) can be performed in a sealed state, the safety of the worker is ensured, and the substance affecting the analysis is prevented from being mixed into the specimen from the worker. In addition, the used detection device can be easily discarded.

FIG. 2 is an explanatory diagram of the first container 14. 2A is a perspective view of the first container 14 in a state where the sample processing solution 15 is put and the lid 16 is closed, and FIG. 2B is a cotton swab so that the cotton ball 25 enters the sample processing solution 15. 2 is a front view showing a state before the lid 16 is closed, and FIG. 2 (c) is a view of the first container 14 of FIG. 2 (b). FIG. In this embodiment, six cuts 29 are provided radially from the center of the bottom surface on the bottom surface side of the back 28 shown in FIG.
FIG. 3 shows an explanatory diagram of the second container 17. 3A is a front view showing a state before the immunochromatographic carrier 19 is inserted and the lid 20 is closed, and FIG. 3B is a partition member of the immunochromatographic carrier 19 (only one is shown). 18 is a front view showing a state after being inserted between 18 and after the lid 20 is closed. FIG.
FIG. 4 is an explanatory view showing the multi-item detection device 30 of the present invention in which the first container 14 is housed in the second container 17 and the lid 20 is closed (the immunochromatographic carrier 19 is not shown). The back 28 of the first container 14 is pressed and broken by the protrusion 24, and the sample extract 15 ′ contained in the first container 14 flows out into the second container 17, and the sample is extracted from the lower end of the immunochromatographic carrier 19. The extract 15 'penetrates.

FIG. 5 shows a cross-sectional view taken along the line II of FIG. 3B [FIG. 5A]. 5B is a cross-sectional view of the immunochromatographic carrier 19 in FIG. 5A, and FIG. 5C is a cross-sectional view of the partition member 33 in FIG. 5A. The angle θ formed by the two partition members 18 is appropriately selected according to the number of partition members 18, for example, 50 °. The partition member 18 can be radially arranged on the inner wall surface 31 of the second container 17 along the circumferential direction with respect to the center. The inner wall surface 31 and / or the inner back surface 23 of the second container 17 can be provided with means for locking the partition member 18, such as a groove or a recess. The partition member 18 may be integrally formed with the second container 17.
The size, shape, and number of the partition members 18 are appropriately selected according to the size, shape, and number of immunochromatographic carriers 19 disposed between the partition members 18. For example, the partition member 18 may be a strip having a rectangular cross section perpendicular to the longitudinal direction. Alternatively, the section perpendicular to the longitudinal direction may be an L-shaped strip like the partition member 32, or the section perpendicular to the longitudinal direction may be a T-shaped strip like the partition member 33. It's okay.
The outer diameter L ₁ of the second container 17 is appropriately selected so that the first container 14 can be accommodated in the second container 17. The outer diameter L ₂ of the second container 14 is a maximum up to a diameter L _{2 of a} circle in contact with the inner ends of the partition members 18, 32, 33. For example, L ₁ is 18.0 mm and L ₂ is 12.0 mm.
The vertical dimension L ₃ and the horizontal dimension L ₄ of the immunochromatographic carrier 19 are selected so that the immunochromatographic carrier 19 can be fixed and arranged between the two partition members 32 or 33 as shown in FIG. For example, L ₃ is 5.0 mm and L ₄ is 1.0 to 2.0 mm.
The dimensions of each part of the partition member 33 shown in FIG. 5C are, for example, L ₅ is 1.5 mm, L ₆ is 2.0 mm, and L ₇ is 0.7 mm.

FIG. 6 is a diagram for explaining how to use the multi-item analyzer 34 of the present invention. In addition to the multi-item detection device 30 of the present invention, the multi-item analysis device 34 includes a charge-coupled device (CCD) image sensor 35 that reads data relating to a test item from one or more immunochromatographic carriers 19 of the multi-item detection device 30. And a barcode reader 36 for confirming that one or more immunochromatographic carriers 19 have reached a position where data can be read.
As shown in FIG. 4, the multi-item detection device 30 of the present invention in a state where the first container 14 is accommodated in the second container 17 and the lid 20 is closed is shown in FIG. It is attached and fixed to the turntable 37 of 34. Next, the multi-item analyzer 34 is rotated clockwise as indicated by a small arrow in the figure by rotating the turntable 37 with a motor 38. The turntable 37 is repeatedly paused and restarted so that data is sequentially read from one or more immunochromatographic carriers 19 by a control means (for example, a personal computer; not shown). Data read by the sensor 35 is recorded in a recording means (for example, a hard disk or a USB memory; not shown).
If desired, the detection results, analysis results, etc. are displayed on a display connected to a personal computer and printed by a printing machine.

Example 2:
FIG. 7 is an explanatory diagram of a horizontal multi-item detection apparatus according to the second embodiment of the present invention. When the first container 39 is accommodated in the L-shaped second container 40 and the lid (not shown) is closed, the back 41 of the first container 39 is provided on the inner back surface 42 of the second container 40. 43 is pushed and destroyed, or at least a part is broken. The specimen extract (not shown) leaking from the first container 39 accumulates in the lower part of the vertical portion 44 of the second container 40 and permeates the L-shaped immunochromatographic carrier 45 (only one is shown). Hereinafter, the test item is detected in the same manner as in the vertical multi-item detection apparatus of the first embodiment. Two or more immunochromatographic carriers 45 may be used.
Instead of providing an L-shaped second container 40 and providing a vertical portion 44 for storing the sample extract, at least one recess for storing the sample extract is provided at one end (back side) of the second container 40. Alternatively, an absorbent substance (for example, sponge or filter paper) may be disposed at one end (back side) of the second container 40 to absorb the specimen extract.
As in the case of the first embodiment, the immunochromatographic carrier 45 may use a casing (for example, sandwiching both opposing surfaces with a transparent or opaque polymer sheet or the like).
When visually judging the detection result of the immunochromatography method, the position of the detection result is read from the immunochromatographic carrier using the horizontal multi-item detection device rather than using the vertical multi-item detection device. It is easy and can judge a detection result correctly.

FIG. 1 is an exploded perspective view of the multi-item detection apparatus according to the first embodiment of the present invention. FIG. 2 is an explanatory diagram of a sealable first container that can enclose a sample processing liquid in the multi-item detection apparatus of FIG. FIG. 3 is an explanatory diagram of a sealable second container that houses the first container in the multi-item detection device of FIG. 1. FIG. 4 is a front view showing a state in use of the multi-item detection device of the present invention. FIG. 5 is a diagram for explaining the arrangement of immunochromatographic carriers in the multi-item detection apparatus of the present invention. FIG. 6 is a diagram for explaining how to use the multi-item analyzer of the present invention. FIG. 7 is an explanatory diagram of the multi-item detection apparatus according to the second embodiment of the present invention.

Explanation of symbols

14, 39: First container 15: Specimen treatment liquid 15 ': Specimen extract 16, 20: Lid 17, 40: Second container 18, 32, 33: Partition member 19: Immunochromatographic carrier 21: Lower surface 22: Convex 23 , 42: inner back surface 24, 43: protrusion 25: cotton ball 26: cotton swab 27: upper surface 28, 41: back 29: notch 30: multi-item detection device 31: inner wall surface 34: multi-item analysis device 35: sensor 36: Bar code reader 37: Turntable 38: Motor 44: Vertical section

Claims (5)

Specimen extraction consisting of a sealable first container capable of enclosing a sample treatment liquid and a sealable second container containing the first container, wherein the sample is introduced into the sample treatment liquid and the test substance is extracted A multi-item detection apparatus for detecting one or more test items with respect to a test substance by simultaneously applying one or more immunochromatographic carriers for the liquid,
The first container comprises a sample collection rod and a sample processing solution,
The second container is an immunochromatographic carrier that detects the test item by specifically reacting with a test substance in the sample extract when the sample extract permeates, and the individual immunochromatographic carriers are different from each other. One or more immunochromatographic carriers capable of detecting the type of test item are provided, and when the first container is stored in the second container, the back of the first container is broken and the specimen extract leaks. A multi-item detection apparatus comprising a specimen extract leakage means for exiting. As a leakage means of the specimen extract,
A notch is provided in the back of the first container, and a protrusion for pressing and breaking the bottom of the first container on the inner back surface of the second container, and
When the first container is stored in the second container, the lid of the second container is closed and the second container is sealed, and the back of the first container is broken so that the sample extract leaks out. The multi-item detection apparatus according to claim 1, wherein a convex portion for pressing the upper surface of the lid of the first container is provided on the lower surface of the lid of the second container. The multi-item detection apparatus according to claim 1, wherein the one or more immunochromatographic carriers are arranged in a circumferential direction on the inner peripheral surface of the second container;
A fixed / rotating means for rotating the attached multi-item detection device so that data relating to a test item can be read from one or more immunochromatographic carriers,
Alignment means for confirming that the one or more immunochromatographic carriers have reached a position where the data can be read;
A sensor that reads the data from the one or more immunochromatographic carriers;
Control means for repeating the pause and restart of the fixing / rotating means so that the data is sequentially read from the one or more immunochromatographic carriers;
A multi-item analyzer comprising recording means for recording data obtained from the sensor.   4. The multi-item analyzer according to claim 3, wherein the sensor is a charge coupled device (CCD) image sensor, and the alignment means is a barcode reader.   4. The multi-item analyzer according to claim 3, wherein the fixing / rotating means comprises a turntable and a motor.

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