WO2022213383A1

WO2022213383A1 - Closed nucleic acid test consumable and test method

Info

Publication number: WO2022213383A1
Application number: PCT/CN2021/086255
Authority: WO
Inventors: 洪俊安; 周诗寒
Original assignee: 广州市达安医疗器械有限公司
Priority date: 2021-04-09
Filing date: 2021-04-09
Publication date: 2022-10-13

Abstract

Disclosed in the present invention is a closed nucleic acid test consumable, comprising a connector, a sample tube, and a reaction tube. The connector is provided with a sample puncture tube and a reaction puncture tube which form a certain angle with the connector; the sample puncture tube and the reaction puncture tube are located on a same side of the connector and are respectively located at the two ends of the connector; the connector is provided with a flow channel for communicating the sample puncture tube with the reaction puncture tube; the connector is provided with a first positioning seat connected to the sample puncture tube and a second positioning seat connected to the reaction puncture tube; and the reaction tube is connected to the second positioning seat in an inserted mode, such that fully-closed connection is achieved, leakage of samples is prevented, infection is avoided, and the samples can be prevented from being contaminated; moreover, quantitative transfer and retention of the samples can be achieved; and the consumable is high in practicability and suitable for mass production. In addition, the present invention also provides a closed nucleic acid test method; by means of two times of centrifugation, the samples are rotated from the sample tube to the flow channel and then transferred into the reaction tube by using the consumable.

Description

A kind of closed nucleic acid detection consumables and detection method

technical field

The present invention relates to the technical field of nucleic acid detection, in particular to a closed nucleic acid detection consumable and a detection method.

Background technique

In the field of PCR detection of infectious diseases, sample preservation solution or nucleic acid release agent is usually pre-injected into the sample tube, then the collected sample is put into the sample tube, the sample tube is sealed, and then sent to the testing site for testing. During detection, it is necessary to reopen the cap of the sample tube for sampling, and drop the quantitative sample solution into the reaction tube containing the nucleic acid amplification reaction reagent. Since the cap of the sample tube is opened and comes into contact with the external environment, there is a risk that the testing personnel will be infected or the sample will be contaminated. In addition, testing is usually carried out in places with corresponding protection levels, which is not conducive to the rapid diagnosis and screening of infectious diseases.

Chinese invention patent CN102534011A (published on July 4, 2012) discloses a fully enclosed method and device for rapid fluorescence detection of target nucleic acid amplification products. In the closed device, the reaction tube is broken, so that the amplification product in the reaction tube reacts with the detection solution preset in the closed device, and then fluorescence detection is performed to interpret the result. The device includes a PCR tube, a centrifuge tube and a thimble plug, the PCR tube can be placed in the centrifuge tube, the thimble plug has a puncture needle capable of piercing the PCR tube, and the centrifuge tube has a sealable cap. In this patent, the PCR tube is put into the centrifuge tube, and when the cap of the centrifuge tube is closed, the tube cap will put pressure on the PCR tube, and the thimble plug in the centrifuge tube will pierce the PCR tube, causing the solution in the PCR tube to fall. into a centrifuge tube to achieve solution transfer in a sealed environment. However, this patent is to realize the transfer of the amplified nucleic acid solution. It does not disclose how the sample solution put into the sample tube after sampling is transferred to the PCR tube, and the fluorescent PCR solution has been used in the existing PCR tube, so no transfer is required. into the centrifuge tube. If the sample solution in the sample tube is transferred to the PCR reaction tube through the patented structure, it can be transferred in a closed environment to avoid infection, but the patented structure will make all the solution in the sample tube enter the PCR reaction tube, resulting in Excessive samples will affect the nucleic acid test results, and the sample solution cannot be retained for repeated testing.

SUMMARY OF THE INVENTION

The purpose of the present invention is to provide a closed nucleic acid detection consumable and a detection method that can quantitatively transfer samples and retain samples.

In order to achieve the above purpose, the present invention provides a closed nucleic acid detection consumable, including a connector, a sample tube and a reaction tube, and the connector is provided with a sample puncture tube and a reaction puncture tube at a certain angle. The puncture tube and the reaction puncture tube are located on the same side of the connecting body and are respectively located at both ends of the connecting body, and the connecting body is provided with a flow channel connecting the sample puncturing tube and the reaction puncturing tube, The connecting body is provided with a first positioning seat and a second positioning seat, the sample puncturing tube passes through the first positioning seat, the sample tube is plugged and connected with the first positioning seat, and the reaction The puncture tube passes through the second positioning seat, and the reaction tube is plug-connected with the second positioning seat.

As a preferred solution, the reaction puncture tube is communicated with one end of the flow channel, and the connection between the sample puncture tube and the flow channel is at a certain distance from the other end of the flow channel, so that the sample puncture tube and the flow channel are connected at a certain distance. The communication of the flow channels forms a dosing chamber between the other ends of the flow channels.

As a preferred solution, the sample puncturing tube is provided with a main pipeline and a connecting pipeline, the main pipeline is communicated with the flow channel through the communication pipeline, and the diameter of the communication pipeline is smaller than the diameter of the main pipeline.

As a preferred solution, a liquid leakage groove is provided on the bottom surface of the flow channel, and the liquid leakage groove is located at one end of the reaction puncturing tube connected with the connecting body, and the reaction puncturing tube is communicated with the liquid leakage groove.

As a preferred solution, the sample tube is threadedly connected to the first positioning seat, and the reaction tube is screwed to the second positioning seat.

As a preferred solution, the sample tube is snap-connected with the first positioning seat, and the reaction tube is snap-connected with the second positioning seat.

As a preferred solution, a side of the connector facing away from the sample puncture tube and the reaction puncture tube is a transparent plate.

As a preferred solution, a plurality of the connecting bodies, the sample tubes and the reaction tubes are provided, and a plurality of the connecting bodies are arranged in parallel and connected in sequence to form a whole, so that the plurality of the sample tubes are arranged in a row at the same location. On one side of the whole, the reaction tubes are arranged in a row on the other side of the whole.

The present invention also provides a closed nucleic acid detection method, comprising the following steps:

(1) A sample puncture tube and a reaction puncture tube are respectively vertically connected at both ends of the connector body, and a flow channel connecting the sample puncture tube and the reaction puncture tube is provided in the connector body, wherein the reaction puncture tube is The puncture tube is communicated with one end of the flow channel, and the communication place between the sample puncture tube and the flow channel has a certain distance from the other end of the flow channel, so that the communication place between the sample puncture tube and the flow channel is A quantitative chamber is formed between it and the other end of the flow channel;

(2) Insert the sample tube containing the nucleic acid sample into the connector, make the sample puncture tube penetrate into the sample tube, and insert the reaction tube containing the nucleic acid amplification reaction reagent into the connector. On the connector, the reaction puncture tube is inserted into the reaction tube, wherein the length of the reaction puncture tube inserted into the reaction tube is greater than the height of the nucleic acid amplification reaction reagent in the reaction tube;

(3) Place the connecting body, the sample tube and the reaction tube at an incline or horizontally, so that the quantitative chamber and the bottom end of the reaction tube are on the same straight line, and use the A certain point on the straight line where the quantitative chamber and the reaction tube are located is the first rotation center, wherein the bottom end of the reaction tube is closer to the first rotation center than the quantitative chamber, and then goes around the a first rotation center, for centrifuging the whole connected body, the sample tube and the reaction tube, so that the sample tube is transferred into the quantitative chamber;

(4) Invert the entire connected body, the sample tube and the reaction tube, and select a point on a straight line that intersects the connector and passes through the bottom end of the reaction tube as the second rotation center , the quantitative chamber is closer to the second rotation center relative to the bottom end of the reaction tube, and the second rotation center, the quantitative chamber and the bottom end of the reaction tube are not located on the same straight line, Then, the whole connected body, the sample tube and the reaction tube are centrifuged around the second rotation center, so that the liquid in the quantitative chamber is transferred into the reaction tube.

Compared with the prior art, the beneficial effects of the present invention are:

In the present invention, the sample puncture tube and the reaction puncture tube are arranged on the connecting body. When the sample tube and the reaction tube are respectively inserted into the first positioning seat and the second positioning seat, so that the sample tube, the reaction tube and the connecting body form a whole, the sample The puncture tube pierces the sample tube and extends into the sample tube, and the reaction puncture tube pierces the reaction tube and extends into the reaction tube, so that the sample tube tightened after sampling and the reaction tube containing the PCR reaction reagent can be directly connected without opening the tube cover On the connector, a fully enclosed connection is realized to prevent the leakage of the sample in the sample tube, avoid infection, and prevent the sample from being contaminated; after connecting the sample tube and reaction tube to the connector, it can be inverted, shaken or centrifuged. The solution in the sample tube enters the flow channel through the sample puncture tube, and then the sample solution in the flow channel flows into the reaction tube by means of inversion, shaking or centrifugation. Due to the limited space of the flow channel, the solution in the sample tube does not The sample solution in the sample tube can be quantitatively transferred to the reaction tube. After the sample is transferred, the connector is located above the sample tube and the reaction tube, and the solution in the sample tube will not flow into the reaction tube through the flow channel. In the tube, the unreacted sample can be kept, and the sample tube can be removed and used when it needs to be tested again. The consumable material of the invention is simple in structure and strong in practicability, and is favorable for mass production. In addition, the present invention also provides a closed nucleic acid detection method, in which the sample is rotated from the sample tube to the flow channel of the connector through two centrifugations and then transferred to the reaction tube.

Description of drawings

FIG. 1 is a schematic structural diagram of a closed nucleic acid detection consumable according to Embodiment 1 of the present invention.

2 is an exploded view of the closed nucleic acid detection consumable according to the first embodiment of the present invention.

3 is a cross-sectional view of a closed nucleic acid detection consumable according to Embodiment 1 of the present invention.

FIG. 4 is a schematic structural diagram of the connector according to the first embodiment of the present invention.

FIG. 5 is a schematic structural diagram of the sample tube according to the first embodiment of the present invention.

FIG. 6 is a schematic structural diagram of the reaction tube according to the first embodiment of the present invention.

7 is a schematic structural diagram of a closed nucleic acid detection consumable according to Embodiment 2 of the present invention.

8 is a schematic structural diagram of a closed nucleic acid detection consumable according to Embodiment 3 of the present invention.

FIG. 9 is a schematic diagram of step (3) of the closed nucleic acid detection method according to the first embodiment of the present invention.

10 is a schematic diagram of step (4) of the closed nucleic acid detection method according to the first embodiment of the present invention.

In the figure, 1-connector; 101-flow channel; 102-quantitative chamber; 103-leakage tank; 2-sample tube; 201-buckle hole; 3-reaction tube; 301-buckle edge; 4-sample 401-main pipeline; 402-connecting pipeline; 5-reaction puncture tube; 6-first positioning seat; 7-second positioning seat; 701-positioning block; 702-buckling protrusion; 8-buckling block ; 9 - the first center of rotation; 10 - the second center of rotation.

Detailed ways

The specific embodiments of the present invention will be described in further detail below with reference to the accompanying drawings and embodiments. The following examples are intended to illustrate the present invention, but not to limit the scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "portrait", "horizontal", "top", "bottom", "front", "rear", "left", "right", " The orientations or positional relationships indicated by vertical, horizontal, top, bottom, inside, and outside are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying It is described, rather than indicated or implied, that the device or element referred to must have a particular orientation, be constructed and operate in a particular orientation, and therefore should not be construed as limiting the invention. Furthermore, the terms "first", "second", and "third" are used for descriptive purposes only and should not be construed to indicate or imply relative importance.

In the description of the present invention, it should be noted that the terms "installed", "connected" and "connected" should be understood in a broad sense, unless otherwise expressly specified and limited, for example, it may be a fixed connection or a detachable connection Connection, or integral connection; can be mechanical connection, can also be electrical connection; can be directly connected, can also be indirectly connected through an intermediate medium, can be internal communication between two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood in specific situations.

Also, in the description of the present invention, unless otherwise specified, "plurality" means two or more.

Example 1

As shown in FIG. 1 to FIG. 6 , a closed nucleic acid detection consumable according to a preferred embodiment of the present invention includes a connector 1, a sample tube 2 and a reaction tube 3. The connector 1 is provided with a sample puncture tube at a certain angle. 4 and the reaction puncture tube 5, the sample puncture tube 4 and the reaction puncture tube 5 are located on the same side of the connecting body 1 and are respectively located at both ends of the connecting body 1, and the connecting body 1 is provided with a connection connecting the sample puncturing tube 4 and the reaction puncturing tube 5. The flow channel 101, the connecting body 1 is provided with a first positioning seat 6 and a second positioning seat 7, the sample puncture tube 4 passes through the first positioning seat 6, the sample tube 2 is plugged and connected with the first positioning seat 6, and the reaction puncture The tube 5 protrudes from the second positioning seat 7 , and the reaction tube 3 is connected to the second positioning seat 7 by plugging. In this embodiment, a sample puncture tube 4 and a reaction puncture tube 5 are arranged on the connecting body 1. When the sample tube 2 and the reaction tube 3 are respectively inserted into the first positioning seat 6 and the second positioning seat 7, the sample tube 2 and the reaction tube are respectively inserted. 3. When the connector 1 is formed as a whole, the sample puncture tube 4 pierces the sample tube 2 and extends into the sample tube 2, and the reaction puncture tube 5 pierces the reaction tube 3 and extends into the reaction tube 3, so that the sample tightened after sampling The tube 2 and the reaction tube 3 containing the PCR reaction reagent can be directly connected to the connector 1 without opening the tube cover to achieve a fully closed connection, preventing the sample in the sample tube 2 from leaking out, avoiding infection, and preventing the sample from being contaminated. ; After connecting the sample tube 2 and the reaction tube 3 with the connector 1, the solution in the sample tube 2 can enter the flow channel 101 through the sample puncture tube 4 by means of inversion, shaking or centrifugation, and then by inversion, shaking or Centrifugation, etc., make the sample solution in the flow channel 101 flow into the reaction tube 3. Due to the limited space of the flow channel 101, the solution in the sample tube 2 cannot all flow into the flow channel 101, and quantitative transfer of the sample solution in the sample tube 2 can be realized. The sample solution is transferred to the reaction tube 3, and after the sample is transferred, the connector 1 is located above the sample tube 2 and the reaction tube 3, the solution in the sample tube 2 will no longer flow into the reaction tube 3 through the flow channel 101, and can remain unreacted If the sample needs to be tested again, the sample tube 2 can be removed and taken out for use. The consumable in this embodiment has a simple structure and strong practicability, which is favorable for mass production. In this embodiment, the sample puncturing tube 4 and the reaction puncturing tube 5 are vertically connected to the connecting body 1 .

Further, the reaction puncture tube 5 in this embodiment is communicated with one end of the flow channel 101, and the connection between the sample puncture tube 4 and the flow channel 101 is at a certain distance from the other end of the flow channel 101, so that the sample puncture tube 4 and the flow channel 101 are connected. The connection between the other ends of the flow channel 101 forms a quantitative chamber 102. After connecting the connector 1, the sample tube 2 and the reaction tube 3, during centrifugation, the quantitative chamber 102 is regarded as the farthest end from the rotation center. Under the action of centrifugal force, the sample solution in the sample tube 2 will be thrown into the quantitative chamber 102 and fill the quantitative chamber 102 to realize the quantitative extraction of the sample solution, and then the whole solution in the quantitative chamber 102 is transferred by centrifugation into the reaction tube 3 to achieve quantitative transfer of the sample solution.

Optionally, the sample puncturing tube 4 is provided with a main pipeline 401 and a connecting pipeline 402, the main pipeline 401 is communicated with the flow channel 101 through the communication pipeline 402, and the diameter of the communication pipeline 402 is smaller than the diameter of the main pipeline 401, which can avoid the surface tension of the sample solution. The connection between the sample puncturing tube 4 and the flow channel 101 is blocked, and the flow can be controlled. In addition, the bottom surface of the flow channel 101 in this embodiment is provided with a liquid leakage groove 103. The liquid leakage groove 103 is located at one end of the reaction puncture tube 5 connected to the connecting body 1, and the reaction puncture tube 5 is communicated with the liquid leakage groove 103 to prevent the surface tension of the liquid. , so that the sample solution falls into the reaction tube 5 through the reaction puncture tube 5 more smoothly. Further, the length of the reaction puncture tube 5 in this embodiment is greater than the length of the sample puncture tube 4, which is convenient for identifying the position where the sample tube 2 and the reaction tube 3 are connected, and the sample tube 2 needs to store a throat swab or a nasal swab, and the sample tube The length of 2 is longer than the length of reaction tube 3. When transferring the solution in sample tube 2 to flow channel 101 for inversion, tilting or centrifugation, the solution in reaction tube 3 can touch the mouth of reaction tube 3 and not flow from it. The reaction puncture tube 5 flows into the flow channel 101 , and the solution in the sample tube 2 smoothly enters the flow channel 101 through the sample puncture tube 4 . Optionally, the length of the reaction puncture tube 5 in this embodiment is greater than 0.5 times the length of the reaction tube 3, which can ensure the stock of the reaction reagent and prevent the reaction reagent from flowing into the flow channel when the sample solution is transferred from the sample tube 2 to the flow channel 101. 101.

In this embodiment, the sample tube 2 is threadedly connected to the first positioning seat 6 , and the reaction tube 3 is threadedly connected to the second positioning seat 7 . When the sample tube 2 is screwed on the first positioning seat 6 , the sample puncturing tube 4 pierces the sample tube 2 and into the sample tube 2 , when the reaction tube 3 is screwed on the second positioning seat 7 , the reaction puncturing tube 5 pierces the reaction tube 3 and extends into the reaction tube 3 . In addition, the side of the connecting body 1 in this embodiment away from the sample puncturing tube 4 and the reaction puncturing tube 5 is a transparent plate, which can be used to observe the liquid in the flow channel 101 .

Embodiment 2

As shown in FIG. 7 , the difference between this embodiment and the first embodiment is that the sample tube 2 of this embodiment is snap-connected with the first positioning seat 6 , and the reaction tube 3 is snap-connected with the second positioning seat 7 . The first positioning seat 6 is provided with a snap block 8, and the sample tube 2 is provided with a snap hole 201. When the sample tube 2 is inserted into the first positioning seat 6, the snap block 8 is matched with the snap hole 201, The sample tube 2 can be firmly connected to the first positioning seat 6 . The second positioning base 7 includes two positioning blocks 701 . The two positioning blocks 701 are arranged at intervals to form a slot into which the end of the reaction tube 3 can be inserted. The positioning block 701 is provided with a snap protrusion 702 . One end of the reaction tube 3 A ring of snap edges 301 is provided. When the reaction tube 3 is inserted between the two positioning blocks 701 , the snap edge 301 is located between the snap protrusions 702 and the connecting body 1 , so that the reaction tube 3 and the connecting body 1 are firmly connected. Other structures of this embodiment are the same as those of the first embodiment, and are not repeated here.

Embodiment 3

As shown in FIG. 8 , the difference between this embodiment and the first embodiment is that the connecting body 1 , the sample tube 2 and the reaction tube 3 in this embodiment are all provided in multiples, and the multiple connecting bodies 1 are arranged in parallel and are sequentially connected to form a whole , a plurality of sample tubes 2 are arranged in a row on one side of the whole, and the reaction tubes 3 are arranged in a row on the other side of the whole. Can be used for the detection of multiple samples. Other structures of this embodiment are the same as those of the first embodiment, and are not repeated here.

(1) A sample puncture tube 4 and a reaction puncture tube 5 are vertically connected at both ends of the connector 1, and a flow channel 101 connecting the sample puncture tube 4 and the reaction puncture tube 5 is provided in the connector 1. The puncture tube 5 is in communication with one end of the flow channel 101, and the connection between the sample puncture tube 4 and the flow channel 101 is at a certain distance from the other end of the flow channel 101, so that the connection between the sample puncture tube 4 and the flow channel 101 is connected with the flow channel 101. A quantitative chamber 102 is formed between the other ends;

(2) Insert the sample tube 2 containing the nucleic acid sample into the connector 1, make the sample puncture tube 4 penetrate into the sample tube 2, and insert the reaction tube 3 containing the nucleic acid amplification reaction reagent into the connection On the body 1, the reaction puncture tube 5 is inserted into the reaction tube 3, wherein the length of the reaction puncture tube 5 inserted into the reaction tube 3 is greater than the height of the nucleic acid amplification reaction reagent in the reaction tube 3;

(3) As shown in FIG. 9 , place the connecting body 1, the sample tube 2 and the reaction tube 3 at an incline or horizontally, so that the bottom end of the quantitative chamber 102 and the reaction tube 3 are located on the same straight line, and quantitatively A point on the straight line where the chamber 102 and the reaction tube 3 are located is the first rotation center 9 , wherein the bottom end of the reaction tube 3 is closer to the first rotation center 9 than the quantitative chamber 102 , and then surrounds the first rotation center 9 , centrifuge the whole connected body 1, sample tube 2 and reaction tube 3, so that the sample tube is transferred into the quantitative chamber;

(4) As shown in FIG. 10 , invert the whole connected body 1, sample tube 2 and reaction tube 3, and select a point on a straight line that intersects with connector 1 and passes through the bottom end of reaction tube 3 as the second rotation Center 10, the quantitative chamber 102 is closer to the second rotation center 10 relative to the bottom end of the reaction tube 3, and the second rotation center 10, the quantitative chamber 102 and the bottom end of the reaction tube 3 are not located on the same straight line, and then circle the second rotation center 10. The center 10 is rotated to centrifuge the entire connected body 1, the sample tube 2 and the reaction tube 3, so that the liquid in the quantitative chamber is transferred to the reaction tube.

In the detection method provided by the present invention, after the first centrifugation in step (3), the quantitative sample solution can be retained in the quantitative chamber 102, and the nucleic acid amplification reaction reagent in the reaction tube 3 is gathered on the top of the reaction tube 3, because The length of the reaction puncture tube 5 inserted into the reaction tube 3 is greater than the height of the nucleic acid amplification reaction reagent in the reaction tube 3, the reaction reagent cannot flood the port of the reaction puncture tube 5, and the reagent will not enter the flow channel 101; after step (4) ) the second centrifugation, the sample solution in the quantitative chamber 102 enters the reaction tube 3 along the flow channel 101 and the reaction puncture tube 5 under the action of centrifugal force, and is thrown to the bottom of the reaction tube 3 together with the reaction reagent by centrifugal force, Simultaneously achieve sample and reagent mixing. The sample solution in the sample tube 2 also returns to the bottom of the sample tube 2 under the action of centrifugation. After sampling, the sample tube cover 202 is screwed onto the sample tube body 201, and thereafter the sample tube cover 202 is no longer opened, thereby reducing the risk of infection. Finally, the whole connected with the connector 1, the sample tube 2 and the reaction tube 3 is put into a special PCR instrument, and PCR detection can be performed.

To sum up, the embodiment of the present invention provides a closed nucleic acid detection consumable. By setting the sample puncture tube 4 and the reaction puncture tube 5 on the connector 1, when the sample tube 2 and the reaction tube 3 are respectively connected to the first positioning seat 6, When the second positioning seat 7 is inserted to form a whole of the sample tube 2 , the reaction tube 3 and the connecting body 1 , the sample puncture tube 4 pierces the sample tube 2 and extends into the sample tube 2 , and the reaction puncture tube 5 pierces the reaction tube 3 And extend into the reaction tube 3, so that the sample tube 2 that is tightened after sampling and the reaction tube 3 containing the PCR reaction reagent can be directly connected to the connector 1 without opening the tube cover, so as to realize a fully closed connection and prevent the sample tube 2 from being trapped. The sample is leaked to avoid infection, and it can prevent the sample from being contaminated; after connecting the sample tube 2 and the reaction tube 3 to the connector 1, the solution in the sample tube 2 can be punctured through the sample by inversion, shaking or centrifugation. The tube 4 enters the flow channel 101, and then the sample solution in the flow channel 101 flows into the reaction tube 3 by means of inversion, shaking or centrifugation. Due to the limited space of the flow channel 101, the solution in the sample tube 2 cannot all flow into the reaction tube 3. In the flow channel 101, the sample solution in the sample tube 2 can be quantitatively transferred to the reaction tube 3, and after the sample is transferred, the connector 1 is located above the sample tube 2 and the reaction tube 3, and the solution in the sample tube 2 will not be reused. Flowing into the reaction tube 3 through the flow channel 101, the unreacted sample can be retained, and the sample tube 2 can be removed for use when re-testing is required. The consumable in this embodiment has a simple structure and strong practicability, which is favorable for mass production. In addition, the present invention also provides a method for closed nucleic acid detection, in which the sample solution in the above-mentioned sample tube is transferred to the flow channel of the connector through the first centrifugation, and the sample solution in the flow channel is removed by the second centrifugation. Transfer to a reaction tube.

The above are only the preferred embodiments of the present invention. It should be pointed out that for those skilled in the art, without departing from the technical principle of the present invention, several improvements and replacements can be made. These improvements and replacements It should also be regarded as the protection scope of the present invention.

Claims

A closed nucleic acid detection consumable, characterized in that it comprises a connector, a sample tube and a reaction tube, the connector is provided with a sample puncture tube and a reaction puncture tube at a certain angle, the sample puncture tube and the The reaction puncture tube is located on the same side of the connecting body and at both ends of the connecting body respectively. The connecting body is provided with a flow channel connecting the sample puncturing tube and the reaction puncturing tube. A first positioning seat and a second positioning seat are provided, the sample puncturing tube is pierced from the first positioning seat, the sample tube is plugged and connected with the first positioning seat, and the reaction puncturing tube is inserted from the first positioning seat. The second positioning seat is protruded, and the reaction tube is connected with the second positioning seat by plugging.
The closed nucleic acid detection consumable according to claim 1, wherein the reaction puncture tube is communicated with one end of the flow channel, and the connection between the sample puncture tube and the flow channel is connected with the end of the flow channel. The other end has a certain distance, so that the communication between the sample puncturing tube and the flow channel is between the other end of the flow channel to form a quantitative chamber.
The closed nucleic acid detection consumable according to claim 1, wherein the sample puncturing tube is provided with a main pipeline and a connecting pipeline, the main pipeline is communicated with the flow channel through the communication pipeline, and the communication pipeline The diameter is smaller than the diameter of the main pipe.
The closed nucleic acid detection consumable according to claim 1, wherein the bottom surface of the flow channel is provided with a liquid leakage groove, and the liquid leakage groove is located at the end where the reaction puncture tube is connected with the connecting body, so The reaction puncture tube is communicated with the leakage tank.
The closed nucleic acid detection consumable according to claim 1, wherein the sample tube is screwed with the first positioning seat, and the reaction tube is screwed with the second positioning seat.
The closed nucleic acid detection consumable according to claim 1, wherein the sample tube is snap-connected with the first positioning seat, and the reaction tube is snap-connected with the second positioning seat.
The closed nucleic acid detection consumable according to claim 1, wherein a side of the connector facing away from the sample puncture tube and the reaction puncture tube is a transparent plate.
The closed nucleic acid detection consumable according to claim 1, wherein the connector, the sample tube and the reaction tube are provided in multiples, and a plurality of the connectors are arranged in parallel and connected in sequence to form a whole , a plurality of the sample tubes are arranged in a row on one side of the whole, and the reaction tubes are arranged in a row on the other side of the whole.
A closed nucleic acid detection method, comprising the steps of:

(1) A sample puncture tube and a reaction puncture tube are respectively vertically connected at both ends of the connector body, and a flow channel connecting the sample puncture tube and the reaction puncture tube is provided in the connector body, wherein the reaction puncture tube is The puncture tube is communicated with one end of the flow channel, and the communication place between the sample puncture tube and the flow channel has a certain distance from the other end of the flow channel, so that the communication place between the sample puncture tube and the flow channel is A quantitative chamber is formed between it and the other end of the flow channel;

(2) Insert the sample tube containing the nucleic acid sample into the connector, make the sample puncture tube penetrate into the sample tube, and insert the reaction tube containing the nucleic acid amplification reaction reagent into the connector. On the connector, the reaction puncture tube is inserted into the reaction tube, wherein the length of the reaction puncture tube inserted into the reaction tube is greater than the height of the nucleic acid amplification reaction reagent in the reaction tube;

(3) Place the connecting body, the sample tube and the reaction tube at an incline or horizontally, so that the quantitative chamber and the bottom end of the reaction tube are on the same straight line, and use the A certain point on the straight line where the quantitative chamber and the reaction tube are located is the first rotation center, wherein the bottom end of the reaction tube is closer to the first rotation center than the quantitative chamber, and then goes around the a first rotation center, for centrifuging the whole connected body, the sample tube and the reaction tube, so that the sample tube is transferred into the quantitative chamber;

(4) Invert the entire connected body, the sample tube and the reaction tube, and select a point on a straight line that intersects the connector and passes through the bottom end of the reaction tube as the second rotation center , the quantitative chamber is closer to the second rotation center relative to the bottom end of the reaction tube, and the second rotation center, the quantitative chamber and the bottom end of the reaction tube are not located on the same straight line, Then, the whole connected body, the sample tube and the reaction tube are centrifuged around the second rotation center, so that the liquid in the quantitative chamber is transferred into the reaction tube.