CN212357257U - Anti-pollution detection device for rapid detection of nucleic acid amplification product - Google Patents
Anti-pollution detection device for rapid detection of nucleic acid amplification product Download PDFInfo
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- CN212357257U CN212357257U CN202021162806.8U CN202021162806U CN212357257U CN 212357257 U CN212357257 U CN 212357257U CN 202021162806 U CN202021162806 U CN 202021162806U CN 212357257 U CN212357257 U CN 212357257U
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Abstract
The utility model discloses an anti-pollution detection device for nucleic acid amplification thing short-term test, including detection portion and mixed reaction cabin, mixed reaction cabin includes: reaction chamber main part, reaction hatch cover and reaction chamber main part sealing connection can be dismantled, are equipped with the passageway of intercommunication hybridization liquid holding part and mixed reaction portion between reaction hatch cover and the reaction chamber main part, and the detection portion is including detecting the inner core and holding the detection box body that detects the inner core, is provided with transparent observation window corresponding to the reaction zone position that detects the inner core on the detection box body, still is equipped with the guiding device who matches with mixed reaction chamber on the detection box body, and the guiding device includes: the device comprises a flow guide pipe and a flow guide component, wherein one end of the flow guide component is connected with the flow guide pipe, the other end of the flow guide component is connected with the detection inner core, the hybridization liquid and the sample mixed liquid are guided to the detection inner core, and the detection inner core displays a final detection result in an area corresponding to the transparent observation window. And has the advantages of good sealing effect and prevention of primer aerosol pollution.
Description
Technical Field
The utility model relates to a nucleic acid detects the field, especially relates to an anti-pollution detection device that is used for nucleic acid amplification thing short-term test.
Background
The novel coronavirus belongs to the genus beta coronavirus, has envelope, and has round or elliptical particle, usually polymorphism, and diameter of 60-140 nm. It is clearly distinguished from SARSr-CoV and MERSR-CoV on the basis of characteristics. The present research shows that the homology with bat SARS-like coronavirus (bat-SL-CoVZC45) reaches more than 85%. Based on current epidemiological investigation, the incubation period is 1-14 days, and is mostly 3-7 days. The clinical manifestations are mostly mild, fever, dry cough and hypodynamia are taken as main manifestations, but severe patients have dyspnea and/or hypoxemia after one week, and severe patients can rapidly progress to acute respiratory distress syndrome, septic shock, metabolic acidosis which is difficult to correct, coagulation dysfunction, multiple organ failure and the like. Therefore, the early discovery, the early treatment and the early isolation can effectively prevent the spread of diseases and reduce the proportion of severe and critically ill patients.
Nucleic acid extraction has become the most important and basic operation in molecular biology experimental technology, but at present, the extraction scheme of viral nucleic acid is very many and is complicated and simple. The basic population of the population is often the starting point for outbreaks of infectious diseases, and there is a great need to be able to quickly diagnose nucleic acids in situ, which is of great significance in the prevention and control of infectious diseases.
However, according to the detection level and treatment conditions of the field or the basement, a new crown nucleic acid rapid detection product suitable for community and individual field detection is urgently needed to be developed, the detection pressure of medical institutions in severe epidemic areas is relieved, and the cross infection risk of the detected people is reduced.
SUMMERY OF THE UTILITY MODEL
In order to solve the problems of the prior art, in one aspect, the present invention provides an anti-pollution detection device for rapidly detecting a nucleic acid amplification product, comprising a detection part and a mixed reaction chamber,
the mixing reaction chamber comprises:
a reaction cabin main body, wherein a hybridization solution containing part and a mixed reaction part are arranged in the reaction cabin main body, the hybridization solution containing part and the mixed reaction part are respectively provided with a chamber for containing a hybridization solution and a sample to be detected,
the reaction cabin cover is hermetically connected with and detachable from the reaction cabin main body, a channel for communicating the hybridization solution accommodating part with the mixed reaction part is arranged between the reaction cabin cover and the reaction cabin main body, and when the mixed reaction cabin which is filled with the hybridization solution and the sample in an isolated manner is inverted, the hybridization solution and the sample are subjected to mixed reaction through the channel; the reaction cabin cover is also provided with a flow guide opening penetrating through the reaction cabin cover, and the flow guide opening is provided with a sealing cap capable of being punctured;
the detection portion is provided with transparent observation window including detecting the inner core and holding the detection box body that detects the inner core, the reaction zone position that corresponds to detecting the inner core on the detection box body, still be equipped with the guiding device who matches with mixed reaction cabin on the detection box body, the guiding device includes:
a guide tube for puncturing the sealing cap and guiding the hybrid liquid and the sample mixed liquid after the mixing reaction to the guide part,
and one end of the diversion component is connected with the diversion pipe, the other end of the diversion component is connected with the detection inner core, the hybridization liquid and the sample mixed liquid are diverted to the detection inner core, and the detection inner core displays a final detection result in an area corresponding to the transparent observation window.
Furthermore, the detection box body comprises an upper shell and a lower shell, the upper shell and the lower shell are hermetically connected, a detection cavity for accommodating the detection inner core is formed between the upper shell and the lower shell, and the detection inner core is arranged in the detection cavity;
the upper surface of the upper shell is provided with the flow guide device.
Furthermore, the guiding device still includes fixing base and end cap, the honeycomb duct install in the fixing base runs through the fixing base, it breaks the water conservancy diversion hole to be equipped with on the last casing to wipe, the fixing base cover is established and is located to wipe and breaks the water conservancy diversion hole and outside the detection box body, the end cap is fixed or can be dismantled with the fixing base from last casing inboard and is connected honeycomb duct fixed connection on last casing, water conservancy diversion part one end is installed on the end cap to contact with the honeycomb duct.
Furthermore, the diversion component is a water guide fiber membrane, and the water guide fiber membrane extends to the detection inner core from the lower end of the diversion pipe.
Further, the detection inner core is a nucleic acid colloidal gold test strip.
Further, the guiding device still is equipped with totally closed or semi-closed by the upward annular installation department that extends of last casing surface, annular installation department with the outer shape size of mixed reaction cabin matches, but mixed reaction cabin card is established and is installed in the annular installation department.
Further, it sets up on last casing and is located to wipe brokenly the water conservancy diversion hole be close to transparent observation window one side in the annular installation department, go up on the casing and be located the opposite side of keeping away from transparent observation window in the annular installation department is provided with mixed reaction portion fixed orifices, mixed reaction portion is by the reaction cabin main part lower surface outwards extension outstanding reaction zone protruding portion that forms, the reaction zone protruding portion with the size of reaction portion fixed orifices matches.
Furthermore, the reaction zone protrusion is a combination of a cylinder and a cone, and the cavity for accommodating liquid therein is also a combination of a cylinder and a cone.
Furthermore, the flow guide opening is arranged above the corresponding mixing reaction part.
Furthermore, the hybridization solution containing part comprises a detachable hybridization solution container, a partition board is arranged in the reaction chamber body to divide the interior of the reaction chamber body into two chambers, one side is a mixed reaction part for containing a sample, and the other side is a hybridization solution container arrangement part for placing the hybridization solution container.
Further, the hybridization solution container is pre-packaged and sealed, and the opening of the hybridization solution container is sealed by a sealing film which can be torn off.
The utility model also provides an anti-pollution detection method of nucleic acid amplification thing short-term test, adopt above-mentioned arbitrary an anti-pollution detection device for nucleic acid amplification thing short-term test, then include following step:
a) opening a reaction hatch cover, adding a hybridization solution into a hybridization solution accommodating part, putting a PCR probe into a mixed reaction part, and then adding a sample to be detected into the mixed reaction part;
b) covering a reaction hatch cover, and carrying out isothermal amplification on the sample to be detected in the mixed reaction part;
c) then the mixed reaction cabin is inverted and inclined, so that the hybridization liquid and the sample flow uniformly into the cavity of the mixed reaction part corresponding to the flow guide port under the action of gravity to carry out mixed reaction, the mixed reaction cabin is inverted and placed on the upper shell after being mixed, the flow guide port is aligned with the flow guide pipe, the flow guide pipe punctures the sealing cap and guides the mixed liquid of the hybridization liquid and the sample after the mixed reaction into the flow guide component, the flow guide component guides the mixed liquid of the hybridization liquid and the sample to the detection inner core, and the detection inner core displays the final detection result in the area corresponding to the transparent observation window.
Further, d) discarding the whole nucleic acid amplification product in a safe place without disassembling the anti-contamination detecting apparatus for rapid detection of the nucleic acid amplification product after detection.
The utility model also provides an above-mentioned arbitrary anti-pollution detection device that is used for nucleic acid amplification thing short-term test or above-mentioned arbitrary one nucleic acid amplification thing short-term test's anti-pollution detection method in the aspect of food industry, agriculture, animal husbandry, customs quarantine, gene mutation detection and DNA mononucleotide polymorphism identification.
The utility model discloses following beneficial effect has:
1. the utility model relates to an anti-pollution detection device for rapid detection of nucleic acid amplification products, which realizes that liquid in a mixed reaction chamber flows out to a detection inner core in a completely closed environment, and has the advantages of good closing effect and prevention of primer aerosol pollution;
2. the utility model relates to an anti-pollution detection device and method for rapid detection of nucleic acid amplification product, which can prevent pollution in the detection process due to good sealing performance, can realize rapid real-time detection according to the detection and treatment conditions of the site or the basic level, and has convenient operation, simple use and low specialized requirement;
3. the utility model relates to an anti-pollution detection device, method and application for nucleic acid amplification thing short-term test have the new hat nucleic acid short-term test product that is fit for community, individual witnessed inspections, alleviate serious regional medical institution detection pressure of epidemic situation, reduce the advantage of the person's of being examined cross infection risk.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
FIG. 1 is an exploded view of an example of a hybrid reaction chamber of the present invention;
FIG. 2 is a schematic view of the mixing reaction chamber of the present invention when inverted;
FIG. 3 is a schematic view of the mixing reaction chamber of the present invention when it is upright;
fig. 4 is an exploded view of an example of the detecting portion of the present invention;
fig. 5 is a schematic view of the present invention in use;
FIG. 6 is a schematic view of a hybridization solution container according to the present invention;
FIG. 7 is a schematic diagram of isothermal amplification of a sample after adding a hybridization solution and the sample to a mixed reaction chamber according to the present invention;
FIG. 8 is a schematic diagram of the present invention inverting the mixing chamber after isothermal amplification to mix the hybridization solution and the sample;
FIGS. 8-10 are schematic diagrams illustrating the inverted mixing reaction chamber being inserted into the detecting portion for piercing and guiding after the mixing reaction of the present invention is completed to obtain the final result;
fig. 11-12 are schematic diagrams illustrating the present invention in which the mixing reaction chamber and the detecting part are mounted in a snap-fit manner for easy transportation.
The examples in the figures are represented as: a mixing reaction chamber 1; a reaction chamber 11; a reaction hatch 12; a mixing reaction section 111; a hybridization solution container 112; a reaction zone protrusion 113; a hybridization solution container setting section 114; a diversion opening 121; a sealing cap 122; an upper case 21; a lower case 22; a seal ring 23; an inspection inner core 24; a fixing clip 241; a flow guide member 242; a transparent viewing window 26; a viewing window seal card 261; a flow guide pipe 27; sample 4; a metal bath device 5; 6, hybridizing liquid; convex edge 7
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention clearer, the following description will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
As shown in FIGS. 1 to 5, an antipollution device for rapid detection of a nucleic acid amplification product comprises a detection section and a mixing reaction chamber 1,
the mixing reaction chamber comprises:
a reaction cabin main body 11, wherein a hybridization solution containing part and a mixed reaction part 111 are arranged in the reaction cabin main body 11, the hybridization solution containing part and the mixed reaction part 111 are respectively provided with a chamber for containing a hybridization solution and a sample to be detected,
the reaction cabin cover 12 is hermetically connected with and detachable from the reaction cabin main body 11, a channel for communicating the hybridization solution accommodating part with the mixed reaction part is arranged between the reaction cabin cover 12 and the reaction cabin main body 11, and when the mixed reaction cabin 1 which is filled with the hybridization solution and the sample in an isolated manner is inverted, the hybridization solution and the sample are subjected to mixed reaction through the channel; a flow guide opening 121 penetrating through the reaction hatch cover is further formed in the reaction hatch cover 12, and a sealing cap 122 capable of being pierced is arranged on the flow guide opening 121;
detection portion is provided with transparent observation window 26 including detecting inner core 24 and the detection box body that holds detection inner core 24 corresponding to the reaction zone position that detects the inner core on the detection box body, still be equipped with the guiding device who matches with mixed reaction cabin on the detection box body, the guiding device includes:
a guide tube 27 for piercing the sealing cap 122 and guiding the mixed solution of the hybridization solution and the sample after the mixing reaction to the guide member 242,
and the diversion component 242 has one end connected with the diversion pipe 27 and the other end connected with the detection inner core 24, diverts the hybridization solution and the sample mixed solution to the detection inner core 24, and displays the final detection result in the region corresponding to the transparent observation window 26 by the detection inner core 24.
In practical application, for convenience of assembly, the detection box body comprises an upper shell 21 and a lower shell 22, wherein the upper shell 21 and the lower shell 22 are connected in a sealing manner, and a sealing ring 23 is arranged between the upper shell 21 and the lower shell 22 in the example of fig. 1 to achieve the effect of sealing connection. A detection cavity for accommodating the detection inner core 24 is formed between the two, and the detection inner core 24 is arranged in the detection cavity; in the example of fig. 1, a plurality of support pieces protruding upward and having the same height are provided on the inner bottom surface of the lower case 22, the detection core 24 is horizontally placed on the support pieces, and the detection core 24 is fixed inside the lower case 22 by the fixing clip 241. In the example of fig. 1, the flow guiding component 242 is L-shaped, the horizontal section is in contact connection with the right end of the detection core, the vertical section is in contact connection with the flow guiding tube 27, and the flow guiding tube 27 is vertically arranged, so that the mixed solution of the hybridization solution and the sample is guided onto the flow guiding component 242 under the action of gravity, and then is infiltrated and transferred onto the detection core 24, and finally the detection result (generally shown by a color band) is displayed on the detection core 24.
The upper surface of the upper shell 21 is provided with the flow guide device.
As shown in fig. 1 and 5, the flow guiding device further includes a fixing seat 271 and a plug 272, the flow guiding pipe 27 is mounted on the fixing seat 271 and penetrates through the fixing seat 271, the upper housing 21 is provided with a wiping flow guiding hole 28, the fixing seat 271 is sleeved in the wiping flow guiding hole 28 and is located outside the detection box, the plug 272 is fixedly or detachably connected with the fixing seat 271 from the inner side of the upper housing 21, the flow guiding pipe 27 is fixedly connected to the upper housing 21, and one end of the flow guiding component 242 is mounted on the plug and is in contact with the flow guiding pipe 27.
In some embodiments, the flow guiding component 242 is a water-guiding fiber membrane extending from the lower end of the flow guiding tube to the inner detection core. The detection core can also be made of other materials with good water conductivity, and the purpose of guiding the mixed solution of the hybridization solution and the sample into the detection core 24 to realize detection can be achieved.
Wherein, the detection inner core 24 can be a nucleic acid colloidal gold test strip.
In some embodiments, the diversion device is further provided with a fully-closed or semi-closed annular installation part 30 extending upwards from the outer surface of the upper shell 21, the annular installation part 30 is matched with the external dimension of the mixing reaction cabin 1, and the mixing reaction cabin 1 can be installed in the annular installation part 30 in a clamping mode. The setting of annular installation department 30 can be so that when carrying out the water conservancy diversion and detecting, mixed reaction cabin 1 can be connected with the water conservancy diversion device is stable, avoids droing and causes external pollution, simultaneously, when the product does not use, and both link together, and the trouble and the increase of cost in packing, transportation and the management of avoiding the two separation to lead to are convenient for pack, transport and manage.
In the examples shown in fig. 1, 5, and 9-12, the abrasion guiding hole 28 is disposed on the upper casing 21 and located on one side of the annular installation portion 30 close to the transparent observation window 26, a mixed reaction portion fixing hole 29 is disposed on the upper casing 21 and located on the other side of the annular installation portion 30 far from the transparent observation window 26, the mixed reaction portion 111 protrudes outward from the lower surface of the reaction chamber main body 11 to form a reaction region protruding portion 113, and the reaction region protruding portion 113 matches with the reaction portion fixing hole 29 in size. Thus, during packaging and transportation of unused products, the mixing reaction chamber 1 is positively clamped on the annular mounting portion 30, and the reaction zone protrusion is just inserted into the reaction section fixing hole 29. The reaction part fixing hole 29 is provided for the purpose of enabling the sample to be independently immersed into the metal bath device for isothermal amplification after the sample is added, and the hybridization solution containing part thereof can keep a proper distance from the metal bath device, thereby not only meeting the detection requirement, but also facilitating the mixed reaction operation after the isothermal amplification is finished.
In the example of the above figures, the reaction zone protrusion 113 is a combination of a cylinder and a cone, and the cavity in which the liquid is contained is also a combination of a cylinder and a cone.
In a specific implementation, the diversion opening 121 is disposed above the corresponding mixing reaction part 111.
In a preferred embodiment, the hybridization solution container includes a detachable hybridization solution container 112, the reaction chamber body is internally provided with a partition plate to divide the interior of the reaction chamber body into two chambers, one is a mixing reaction part 111 for containing a sample, and the other is a hybridization solution container holder 114 for holding the hybridization solution container 112.
Thus, the hybridization solution container 112 can be made in the form of a pre-packaged seal, the opening of which is sealed with a removable sealing film 1121 as shown in FIG. 6. This ensures that the container holding the hybridization solution is kept clean as necessary before the detection is performed, ensuring the accuracy of the detection.
As shown in FIGS. 6 to 10, the present invention also provides an anti-contamination detection method for rapid detection of nucleic acid amplification product, which comprises the steps of:
a) opening the reaction chamber cover 12, adding the hybridization solution into the hybridization solution container 114 (more preferably, after removing the sealing film 1121, adding the hybridization solution into the hybridization solution container 112 as shown in fig. 6), placing the PCR probe into the mixing reaction part 111, and then adding the sample 4 to be detected into the mixing reaction part 111 (in the illustrated example, into the reaction region protrusion 113);
b) the reaction hatch cover 12 is covered, isothermal amplification is carried out on the sample to be detected in the mixed reaction part, as shown in fig. 7, the sample is singly immersed into the metal bath device 5 for isothermal amplification, and the hybridization solution 6 and the hybridization solution containing part can keep a proper distance from the metal bath device 5, so that the detection requirement is met, and the mixed reaction operation after the isothermal amplification is finished is facilitated. In fig. 7, in order to ensure this safety distance, a ring of beads 7 may be provided at the bottom of the reaction chamber body 11.
c) As shown in fig. 8, the mixing reaction chamber 1 is then tilted upside down to make the hybridization solution 6 and the sample 4 flow uniformly into the chamber of the mixing reaction part 111 corresponding to the diversion opening 121 under the action of gravity to perform mixing reaction, after mixing, the mixing reaction chamber 1 is placed upside down on the upper shell 21, the diversion opening 121 is aligned with the diversion tube 27, the diversion tube 27 pierces the sealing cap 122 and guides the mixed solution of the hybridization solution 6 and the sample 4 after mixing reaction onto the diversion component 242, the diversion component 242 guides the mixed solution of the hybridization solution 5 and the sample 4 to the detection core 24, and the detection core 24 displays the final detection result in the area corresponding to the transparent observation window 26.
Further, d) discarding the whole nucleic acid amplification product in a safe place without disassembling the anti-contamination detecting apparatus for rapid detection of the nucleic acid amplification product after detection.
The utility model also provides an above-mentioned arbitrary anti-pollution detection device that is used for nucleic acid amplification thing short-term test or above-mentioned arbitrary one nucleic acid amplification thing short-term test's anti-pollution detection method in the aspect of food industry, agriculture, animal husbandry, customs quarantine, gene mutation detection and DNA mononucleotide polymorphism identification.
The utility model discloses following beneficial effect has:
1. the utility model relates to an anti-pollution detection device for rapid detection of nucleic acid amplification products, which realizes that liquid in a mixed reaction chamber flows out to a detection inner core in a completely closed environment, and has the advantages of good closing effect and prevention of primer aerosol pollution;
2. the utility model relates to an anti-pollution detection device and method for rapid detection of nucleic acid amplification product, which can prevent pollution in the detection process due to good sealing performance, can realize rapid real-time detection according to the detection and treatment conditions of the site or the basic level, and has convenient operation, simple use and low specialized requirement;
3. the utility model relates to an anti-pollution detection device, method and application for nucleic acid amplification thing short-term test have the new hat nucleic acid short-term test product that is fit for community, individual witnessed inspections, alleviate serious regional medical institution detection pressure of epidemic situation, reduce the advantage of the person's of being examined cross infection risk.
Common pathogens include bacteria, fungi, viruses, mycoplasma, chlamydia, parasites, and the like.
The enclosed nucleic acid amplification product detection apparatus can discriminate not only these pathogens but also their subtypes, virulent strains, mutant strains, drug resistance, and the like.
Above-mentioned all optional technical scheme can adopt arbitrary combination to form the optional embodiment of this utility model, and the repeated description is no longer given here.
The above description is only for the preferred embodiment of the present invention, and is not intended to limit the present invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included within the protection scope of the present invention.
Claims (11)
1. An anti-pollution detection device for rapidly detecting a nucleic acid amplification product is characterized by comprising a detection part and a mixing reaction cabin,
the mixing reaction chamber comprises:
a reaction cabin main body, wherein a hybridization solution containing part and a mixed reaction part are arranged in the reaction cabin main body, the hybridization solution containing part and the mixed reaction part are respectively provided with a chamber for containing a hybridization solution and a sample to be detected,
the reaction cabin cover is hermetically connected with and detachable from the reaction cabin main body, a channel for communicating the hybridization solution accommodating part with the mixed reaction part is arranged between the reaction cabin cover and the reaction cabin main body, and when the mixed reaction cabin which is filled with the hybridization solution and the sample in an isolated manner is inverted, the hybridization solution and the sample are subjected to mixed reaction through the channel; the reaction cabin cover is also provided with a flow guide opening penetrating through the reaction cabin cover, and the flow guide opening is provided with a sealing cap capable of being punctured;
the detection portion is provided with transparent observation window including detecting the inner core and holding the detection box body that detects the inner core, the reaction zone position that corresponds to detecting the inner core on the detection box body, still be equipped with the guiding device who matches with mixed reaction cabin on the detection box body, the guiding device includes:
a guide tube for puncturing the sealing cap and guiding the hybrid liquid and the sample mixed liquid after the mixing reaction to the guide part,
and one end of the diversion component is connected with the diversion pipe, the other end of the diversion component is connected with the detection inner core, the hybridization liquid and the sample mixed liquid are diverted to the detection inner core, and the detection inner core displays a final detection result in an area corresponding to the transparent observation window.
2. The anti-contamination detection apparatus for rapid detection of nucleic acid amplification product according to claim 1,
the detection box body comprises an upper shell and a lower shell, the upper shell and the lower shell are hermetically connected, a detection cavity for accommodating a detection inner core is formed between the upper shell and the lower shell, and the detection inner core is arranged in the detection cavity;
the upper surface of the upper shell is provided with the flow guide device.
3. The anti-contamination detection apparatus for rapid detection of nucleic acid amplification product according to claim 1,
the diversion device further comprises a fixing seat and a plug, the diversion pipe is installed on the fixing seat and penetrates through the fixing seat, a wiping diversion hole is formed in the upper shell, the fixing seat is sleeved in the wiping diversion hole and located outside the detection box body, the plug is fixedly or detachably connected with the fixing seat from the inner side of the upper shell, the diversion pipe is fixedly connected onto the upper shell, and one end of the diversion component is installed on the plug and is in contact with the diversion pipe.
4. The anti-contamination detection apparatus for rapid detection of nucleic acid amplification product according to claim 3,
the water guide component is a water guide fiber membrane, and the water guide fiber membrane extends to the detection inner core from the lower end of the flow guide pipe.
5. The anti-contamination detection device for rapid detection of nucleic acid amplification product according to claim 1, wherein the detection core is a nucleic acid colloidal gold test strip.
6. The apparatus according to claim 3, wherein the flow guide device further comprises a fully or semi-closed annular mounting portion extending upward from an outer surface of the upper housing, the annular mounting portion being adapted to the outer dimension of the mixing chamber, and the mixing chamber being adapted to be engaged with the annular mounting portion.
7. The anti-contamination detection device for rapid detection of nucleic acid amplification product according to claim 6, wherein the scraping guide hole is provided on the upper housing and located on one side of the annular installation portion near the transparent observation window, and a mixed reaction portion fixing hole is provided on the upper housing and located on the other side of the annular installation portion far from the transparent observation window, wherein the mixed reaction portion is formed by protruding outward from the lower surface of the reaction chamber main body to form a reaction region protruding portion, and the reaction region protruding portion matches with the size of the reaction portion fixing hole.
8. The apparatus for the rapid detection of nucleic acid amplification product according to claim 7, wherein the reaction region protrusion is a combination of a cylinder and a cone, and the cavity for containing the liquid therein is also a combination of a cylinder and a cone.
9. The apparatus according to claim 1, wherein the flow guide port is provided above the corresponding mixing reaction section.
10. The apparatus for detecting contamination for the rapid detection of a nucleic acid amplification product according to any one of claims 1 to 9, wherein the hybridization solution container section comprises a detachable hybridization solution container, and the reaction chamber body is internally provided with a partition plate for partitioning the interior of the reaction chamber body into two chambers, one of which is a mixing reaction section for containing a sample and the other of which is a hybridization solution container accommodating section for accommodating the hybridization solution container.
11. The contamination-preventive detection device for rapid detection of nucleic acid amplification product according to claim 10, wherein the hybridization liquid container is a pre-packaged seal whose opening is sealed with a peelable sealing film.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202021162806.8U CN212357257U (en) | 2020-06-19 | 2020-06-19 | Anti-pollution detection device for rapid detection of nucleic acid amplification product |
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| CN202021162806.8U CN212357257U (en) | 2020-06-19 | 2020-06-19 | Anti-pollution detection device for rapid detection of nucleic acid amplification product |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021254505A1 (en) * | 2020-06-19 | 2021-12-23 | 安徽为臻生物工程技术有限公司 | Anti-contamination testing device and method for rapid testing of nucleic acid amplification product, and use thereof |
| CN113980797A (en) * | 2021-10-29 | 2022-01-28 | 广东粤港澳大湾区国家纳米科技创新研究院 | Detection device and detection method for nucleic acid amplification product |
-
2020
- 2020-06-19 CN CN202021162806.8U patent/CN212357257U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021254505A1 (en) * | 2020-06-19 | 2021-12-23 | 安徽为臻生物工程技术有限公司 | Anti-contamination testing device and method for rapid testing of nucleic acid amplification product, and use thereof |
| CN113980797A (en) * | 2021-10-29 | 2022-01-28 | 广东粤港澳大湾区国家纳米科技创新研究院 | Detection device and detection method for nucleic acid amplification product |
| CN113980797B (en) * | 2021-10-29 | 2024-05-24 | 武汉纳达康生物科技有限公司 | Detection device and detection method for nucleic acid amplification product |
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