CN104862216B - High flux visualization total enclosing split type LAMP-LFD detection chip device - Google Patents
High flux visualization total enclosing split type LAMP-LFD detection chip device Download PDFInfo
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- CN104862216B CN104862216B CN201410060517.XA CN201410060517A CN104862216B CN 104862216 B CN104862216 B CN 104862216B CN 201410060517 A CN201410060517 A CN 201410060517A CN 104862216 B CN104862216 B CN 104862216B
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Abstract
A kind of high flux visualization total enclosing split type LAMP LFD detection chip device, it is characterized in that being made up of be mixed receipts sample pond, chip reaction box and LFD reaction box, chip reaction box front end is provided with receives, with being mixed, the interface that the distribution openings in sample pond is connected, inside it is provided with reaction amplification pond, reaction amplification is provided with the probe corresponding with object to be checked in pond, and by rear end LFD seal cap sealing；LFD reaction box includes two cavitys and twin flue syringe needle, first cavity is built-in with LFD nucleic acid test strip, buffer and check valve it is provided with in second cavity, second needle tubing of twin flue syringe needle is connected with check valve, first needle tubing is connected with LFD nucleic acid test strip, after twin flue punctures LFD seals lid, opens check valve, buffer is along flowing into reaction amplification pond, and is drawn on LFD nucleic acid test strip by the first needle tubing and realizes LAMP LFD quick visualization and react.The present invention has simple and reasonable, total enclosing, high flux, multiplicity, Mutiple Targets, split type, flexible and convenient operation feature efficiently.
Description
Technical field
The present invention relates to technical field of biological, belong to nucleic acid isothermal amplification technology and the multinomial technology of cross side analysis technology
Integrated biochip technical field, specifically a kind of high flux visualization total enclosing split type LAMP-LFD detection chip dress
Put..
Background technology
Recently as social development, government, society, the common people are more and more higher to the requirement of self and Environmental security., often
The quantity of the sample of the various biological detection that year is carried out all is increasing with surprising rapidity.The need examined soon simultaneously for scene
Ask more and more urgent.
LAMP technology is set up by (2000) such as Japanese Scientists Notomi the earliest, by 6 districts for target gene
Territory and design 4 species-specific primers, utilize and there is the BstDNA polymerase of strand-displacement activity, under constant temperature
(60-65 DEG C) efficiently (0.5-1h) amplification target dna.Compared with current pandemic PCR nucleic acid amplification technologies,
This technology has expensive instruments such as being independent of temperature cycler (PCR), and highly sensitive, and specificity is good, response speed
The advantage such as fast.Therefore, fast for the scene of clinical disease diagnosis, popular antibacterial or virus as a kind of emerging technology
The fields such as speed detection, animal embryo sex identification and gene chip exploitation.In field of virus detection, the most rely on LAMP
Technological development go out for quick diagnosis detection include Hepaitis B virus, influenza virus, sars coronavirus, simple bleb
Exanthema virus is in the method for interior multiple popular virus.In Bacteria Detection field, this technology is also widely used in M tuberculosis bar
Bacterium, escherichia coli, streptococcus pneumoniae, the quick detection of Shigella dysenteriae.Based on the difference of distinguished sequence on Y chromosome,
Hirayama etc. utilize LAMP technology to carry out the quick detection to cattle early embryo sex, LAMP technology are applied
To a brand-new field.Some scientists attempt itself and nucleic acid horizontal mobility test strips (lateral in recent years
Flow dipstick, LFD) detection technique combine (LAMP-LFD) with realize LAMP Site Detection visualization.Make
During obtaining whole detection, operator is independent of the instrument and equipment that task is special, only need to be by the amplified production of LAMP and reagent paper
Bar immerses buffer can realize the detection of target product, and whole operating process is easy, safety.LAMP-LFD technology one
Through coming out, the parent just obtaining numerous scientist looks at.Especially at aquiculture disease detection field, the most become
Merit for taura syudrome (Tarura syndrome virus, TSV), white spot syndrome virus (White spot syndrome virus,
WSSV), the inspection of the virus causing disease such as infectivity muscle necrosis virus (Infectious myonercrosis virus, IMNV)
Survey.Growing along with this technology, two are hindered it the most gradually to highlight in the bottleneck problem of the application of basic unit.
First it is the Aerosol that brings of the susceptiveness of LAMP.Owing to LAMP method is for the extension spirit of target sequence
Quick and amplification amount is big, often to relate to the processes such as sampling of uncapping, therefore in reality during carrying out LFD detection simultaneously
Application process easily causes Aerosol Pollution, in turn results in the false-positive product of testing result.For tackling this problem,
Typically " wanting strict partition, standard operation " is required at present in actual application for LAMP technology.That is, according to
Test block is divided into " template adds district, detection zone for sample treatment district, solution preparation area " by LAMP operating process, and
To be eventually adding in strict accordance with template in operation, the most negative, positive afterwards, concentration from low to high operates.
For the requirement of such stringent, it is difficult to accomplish during actual basic unit uses, so that this technology is existing in basic unit
Field detection is difficult to promote.Next to that high flux, Mutiple Targets problem.Sample can only be carried out one by one by current technology method
, the detection of single target spot.And in actual use, user be often required in the face of be dozens or even hundreds of sample not
Detect with while target spot.Existing LAMP technology just seems helpless.Therefore, reality is the most reasonably avoided
Aerosol Pollution during detection, can solve again the high flux of LAMP technology, the problem of Mutiple Targets simultaneously, become energy
No promotion LAMP technology detects one of unit around not in fast development the final real service of detection field in basic unit
Open, the problem that also can not escape.
Summary of the invention
The technical problem to be solved is to provide the high flux visualization split type LAMP-LFD of total enclosing and detects core
Sheet devices, has simple and reasonable, high flux, multiplicity, Mutiple Targets, flexible and convenient operation feature efficiently.
The present invention solves the technical scheme that above-mentioned technical problem used: a kind of high flux visualization total enclosing is split type
LAMP-LFD detection chip device, it is characterised in that: this detection chip system and device is by the realization that is connected with centrifuge
Uniformly being mixed of batch mixing receives sample pond, the chip reaction box of Split detachable dress and visual LFD reaction box three part group
Become,
The receipts that are mixed sample pond is disc, and its upper surface is provided with the first capillary channel for sample-adding, and the receipts that are mixed are provided with in sample pond
Multilamellar mix homogeneously groove, the external circumferential in the receipts that are mixed sample pond is distributed multiple distribution openings for connecting chip reaction box；
The front end of chip reaction box is provided with receives, with being mixed, the interface that the distribution openings in sample pond is connected, and sets successively in chip reaction box
There are the second capillary channel being connected with interface and reaction amplification pond, are provided with corresponding with object to be checked in reaction amplification pond
Probe, and sealed by the LFD sealing lid of rear end；
LFD reaction box includes the housing of a hollow, and it is double for thrust that LFD seals in lid that the front portion of housing is built-in with one
Pipeline syringe needle, the rear portion of housing is divided into about two airtight cavitys, is wherein configured with for LAMP in the first cavity
The LFD nucleic acid test strip of reacted nucleic acid Visual experiment validation, the second intracavity is airtight has buffering agents, the
The front portion of two cavitys is provided with check valve, and the second needle tubing of twin flue syringe needle is connected with the check valve with the second cavity, and first
The LFD nucleic acid test strip of needle tubing and the first cavity is connected, when the punctures of twin flue syringe needle reacts the LFD in amplification pond
After sealing lid, opening check valve, buffering agents flows into reaction amplification pond along the second needle tubing, and is utilized rainbow by the first needle tubing
Suction principle is drawn on LFD nucleic acid test strip and realizes the reaction of LAMP-LFD quick visualization.
As improvement, described in be mixed receipts sample pond be to be involuted by discoidal upper cover and discoidal base, the first capillary
Pipeline is arranged on and covers, one end of the first capillary channel with mix homogeneously groove and be connected, the other end is connected with sample adding device,
Mix homogeneously groove is arranged on base, if mix homogeneously groove is made up of the segmentation annulus that dried layer is concentric, on segmentation annulus
Being provided with the breach flowing to water jacket for feed liquid from inside groove, distribution openings is radially uniformly arranged on outermost segmentation annulus.
As improvement, described segmentation annulus is three layers, and breach is 3～4 arc notch, and arc notch is equidirectional bending
Be disposed in an evenly spaced relation in segmentation annulus on, the inwall of the outermost segmentation annulus inside distribution openings is provided with parabola shaped
The accommodating groove of shape, the bottom of accommodating groove is provided with pore, and pore is connected by the interface of distribution openings with chip reaction box.
As improvement, described chip reaction box includes the box body of a both ends open, and one end of box body removably seals sheathed
Having in one the adapter being provided with the second capillary channel, interface is arranged on the end of adapter, reaction amplification pond removably sealing shroud
Being located at the other end of box body, the front end in reaction amplification pond is provided with the capillary channel interface being connected with the second capillary channel,
It is additionally provided with in box body and reacts the buffering release pond for additional air backflow that amplification pond is connected.
Improving, described reaction amplification pond includes cylinder blanket, caves inward into cylindrical cavity bottom cylinder blanket again,
Capillary channel interface is arranged on housing forward end and is connected with cylindrical cavity, and probe is arranged on cylindrical empty intracavity, and LFD is close
Capping is airtight thin material, and the upper end of LFD sealing lid is provided with after can being inserted by the twin flue syringe needle of LFD reaction box close
The draw-in groove that envelope connects.
Improving further, the lower end in described buffering release pond is connected with the capillary channel interface in reaction amplification pond, and buffering is unloaded
The upper end in pressure pond is provided with the sealing lid of an elasticity, and this is close presents recessed state when being sealed on non-airtight sample-adding.
Further improving, on the housing of described LFD reaction box, top or side corresponding to the second cavity are provided with unidirectional
Threshold switch.
Further improving, described distribution openings is evenly spaced 12, and described chip reaction box is corresponding 12.
Compared with prior art, it is an advantage of the current invention that: utilize amplification pool space position difference on chip to realize high flux
The detection chip system and device of the multiplicity Mutiple Targets visualization dismantled and assembled split type trace LAMP-LFD of total enclosing differentiates letter
Number effective differentiation, visual result, effect is obvious, it is not necessary to by other expensive instruments, manufactures and all uses work
The production of industry, and corollary equipment, reagent consumptive material, all use existing routine experimental facilities and consumptive material, and application is convenient,
Be suitable for classes of agents detection reaction, be particularly suitable for scene and field detection supporting needs, micro-dosage reagent detection technique,
Provide be effectively ensured for detection product low cost movement, be very beneficial for LAMP-LFD method in the popularization of basic unit and
Application；Can realize as required expanding pond arbitrary spatial arrangements on chip；It is unlimited that structural models has in theory
Extensibility, can complete the most multiple LAMP-LFD according to the actual requirements and effectively expand, thus meet multiple core
Effective quick diagnosis of acid molecule target spot；Judge less than 1h from being loaded onto result, and operation be the most simple and convenient, non-specially
Industry personnel just can be operated by simple demonstration；Owing to using LFD technology, as long as the content of sample can be made as little as to contain
There is a small amount of nucleic acid molecules to find, prevent detection leakage phenomenon, beneficially primary dcreening operation；Owing to using bioinformatics principle
Biochip technology, the sample taked need not separate specially, can be directly added into after extracting high flux visualization total enclosing
In split type LAMP-LFD detection chip device, and reacted by specific probe and verified and detect, be conducive to existing
Field and field are quickly detected, and adapt to the promotion and application of basic unit.
Accompanying drawing explanation
Fig. 1 is the structural representation of the detection chip device of the present invention；
Fig. 2 is that being mixed of the present invention receives the structural representation in sample pond, and wherein a is the structural representation of upper cover, and b is base
Structural representation；
Fig. 3 is the structural representation of the chip reaction box of the Split detachable dress of the present invention；
Fig. 4 is the structural representation of the visualization LFD reaction box of the present invention；
Fig. 5 is the experimental result picture that ten kinds of important food-borne pathogens are quickly analyzed in the embodiment of the present invention detection.
Detailed description of the invention
Below in conjunction with accompanying drawing embodiment, the present invention is described in further detail.
As Figure 1-4, the high flux visualization total enclosing split type LAMP-LFD detection chip device of the present embodiment,
This detection chip system and device is to be realized being mixed of uniform batch mixing with centrifuge to receive sample pond 1, Split detachable dress by being connected
Chip reaction box 2 and visual LFD reaction box 3 three part composition, mix homogeneously device 1 is disc, be by
Discoidal upper cover 11 and discoidal base 12 involute, and wherein upper cover 11 is provided with female thread, on base 12
Being provided with the external screw thread of correspondence, upper cover 11 is threaded connection fixing with base 12, and between upper cover 11 and base 12
It is lined with sealing ring；
Upper cover 11 be provided with for sample-adding the first capillary channel 111, one end of the first capillary channel 111 with mix homogeneously
Groove 122 is connected, and the other end is connected with sample adding device, is used for filling the reagent such as nucleic acid, enzyme and buffer；Base 12
Inside it is provided with three layers of concentric segmentation annulus 123, base 12 forms three mixing slot segmentations 122 by segmentation annulus 123,
Be distributed in the outside circumference of base 12 12 for the distribution openings 121 connecting chip reaction box 2, distribution openings 121 in
Radial being evenly spaced apart is arranged on the outermost segmentation annulus 123 of base 12, and distribution openings 121 is provided with through
Pore 125, outermost segmentation annulus 123 inwall inside distribution openings 121 is provided with the accommodating groove of parabolic shape
126, pore 125 is arranged on the bottom of accommodating groove 126, has 3～4 on inner side and middle segmentation annulus 123
The breach 124 of arc, arc notch 124 is disposed in an evenly spaced relation on segmentation annulus 123 deviously in equidirectional so that
Water jacket is flowed to from inside groove after feed liquid mixing；Chip reaction box 2 includes the box body of a both ends open, and one end of box body is detachable
Ground sealing shroud is provided with in one the adapter 26 being provided with the second capillary channel 21, and the other end of box body is provided with reaction amplification pond 22,
The end of adapter 26 is provided with the interface 27 being connected with distribution openings 121, and reaction amplification pond 22 removably sealing shroud is located at
The other end of box body, the front end in reaction amplification pond 22 is provided with the capillary channel interface 25 being connected with the second capillary channel 21,
It is additionally provided with in box body and reacts the buffering release pond 23 for additional air backflow that amplification pond 22 is connected, second mao
Thin pipe 21 is to use the pipeline composition of interior diameter 0.1～0.9mm, and presents multiloop S type, reaction amplification pond 22
Including cylinder blanket, in circular arc sphere bottom cylinder blanket, sphere and cylinder are rounding off, cylinder blanket
Bottom caves inward into cylindrical cavity, and capillary channel interface 25 is arranged on cylindrical housings front end and cylindrical cavity phase
Connection, is provided with the probe corresponding with object to be checked at cylindrical empty intracavity, and probe is to be noted by biochip point sample instrument
Enter-evaporation is coated in reaction amplification pond, and seal lid 24 by the LFD of rear end and seal, LFD seals lid
24 is airtight thin material, and LFD seals the upper end of lid 24 and is provided with the card can being tightly connected after being inserted by LFD reaction box 3
Groove 28, the lower end in buffering release pond 23 is connected with the capillary channel interface 25 in reaction amplification pond 22, buffers release pond
The upper end of 23 is provided with the sealing lid of an elasticity, and this is close presents recessed state when being sealed on non-airtight sample-adding, when after airtight sample-adding,
Time additional air is back to buffer release pond 23, close closure domes is with release and ensures that additional air is not by outward leakage；
LFD reaction box 3 includes the housing of a hollow, the tapered reducing diameter part in front portion 31 of housing, is built-in with one for stinging
Entering LFD and seal the twin flue syringe needle 6 in lid 24, the rear portion of housing is divided into about two airtight cavitys, and wherein first
The LFD nucleic acid test strip 5 for LAMP reacted nucleic acid Visual experiment validation it is configured with in cavity 33, second
Intracavity 32 is airtight has buffering agents, and the front portion of the second cavity 32 is provided with check valve 4, corresponding to the second chamber on housing
The sidepiece of body 32 is provided with check valve switch 41, the second needle tubing 62 and check valve of the second cavity 32 of twin flue syringe needle 6
4 are connected, and the first needle tubing 61 is connected with the LFD nucleic acid test strip 5 of the first cavity 33, when twin flue syringe needle 6
After the LFD in punctures reaction amplification pond 22 seals lid 24, opening check valve 4, buffering agents is along the second needle tubing
62 flow into reaction amplification pond 22, and it is real to be utilized siphon principle to be drawn on LFD nucleic acid test strip 5 by the first needle tubing 61
Existing LAMP-LFD quick visualization reaction.
Ten kinds of important food-borne pathogens are quickly analyzed detection with inspection by the detection chip device below with the present invention
Test the use effectiveness of the present invention.Basic operational steps is as follows:
1) embedding probe: reaction amplification pond in high flux visualization total enclosing split type LAMP-LFD detection chip device
Embed probe the most in advance.First the reaction the 1st, 2,3,4,5,6,7,8,9,10 expands in pond, uses Bo Aosheng
PersonalArrayer16 people's point sample instrument individual's point sample instrument (model: ADV-I0006ADV) of brilliant core that thing company produces
It is coated for (vibrio cholera, monokaryon Listerella, vibrio parahaemolytious, shigella, Enterohemorrhagic E.coli, wound
Hinder vibrio, yersinia, vibrio alginolyticus, Vibro harveyi, ten kinds of objects of Salmonella be applicable to LAMP
The primer of amplification, and adding the fluorescein that on ring primer, labelling detects for follow-up LFD.11st amplification pond is coated
Standard nucleic acid positive control probe, is not coated nucleic probe in the 12nd amplification pond.
2) sample-adding is sealed: by airtight sample adding device system by the mixing filling of sample to be tested, enzyme and LAMP reaction buffer
To the receipts sample pond 1 that is mixed, more uniformly it is centrifuged in the reaction amplification pond 22 of each chip reaction box 2 by centrifuge,
Complete the airtight sample-adding process of LAMP reaction system.
3) constant-temperature amplification: the detection chip system and device of the present invention is placed in thermostat water bath, constant temperature at 60-65 DEG C
Hatch 40-60min.
4) result judges: punctured in insertion chip reaction box 2 by the twin flue syringe needle 6 of visualization LFD reaction box 3
Reaction amplification pond 22LFD seals inside lid 24, opens the check valve switch 41 on buffer, and buffer is along twin flue
Second needle tubing 62 of syringe needle flows into reaction amplification pond 22, the reactant after LAMP isothermal amplification and LFD test kit
Reaction, and utilized siphon principle to be drawn on LFD nucleic acid test strip 5 reaction by the test strips on the first needle tubing 61, miscellaneous
Hand over immersion to enter nucleic acid horizontal mobility test strips (LFD), color developing detection, it is judged that LAMP amplification, complete LAMP-LFD
Quick visualization reacts.
Experimental result is as it is shown in figure 5, expand reactant and LFD effect in pond 22 in the reaction being coated with object probe
Reaction；LFD detects there is characteristic reaction zone on line, and the LFD corresponding in the amplification pond not being coated nucleic probe
Reaction zone does not occur.
Claims (8)
1. a high flux visualization total enclosing split type LAMP-LFD detection chip device, it is characterised in that: this inspection
Survey chip system device be realized by being connected with centrifuge uniform batch mixing be mixed receive sample pond, Split detachable dress chip anti-
Answer box and visual LFD reaction box three part composition,
The receipts that are mixed sample pond is disc, and its upper surface is provided with the first capillary channel for sample-adding, and the receipts that are mixed are provided with in sample pond
Multilamellar mix homogeneously groove, the external circumferential in the receipts that are mixed sample pond is distributed multiple distribution openings for connecting chip reaction box；
The front end of chip reaction box is provided with receives, with being mixed, the interface that the distribution openings in sample pond is connected, and sets successively in chip reaction box
There are the second capillary channel being connected with interface and reaction amplification pond, are provided with corresponding with object to be checked in reaction amplification pond
Probe, and sealed by the LFD sealing lid of rear end；
LFD reaction box includes the housing of a hollow, and it is double for thrust that LFD seals in lid that the front portion of housing is built-in with one
Pipeline syringe needle, the rear portion of housing is divided into about two airtight cavitys, is wherein configured with for LAMP in the first cavity
The LFD nucleic acid test strip of reacted nucleic acid Visual experiment validation, the second intracavity is airtight has buffering agents, the
The front portion of two cavitys is provided with check valve, and the second needle tubing of twin flue syringe needle and the check valve of the second cavity are connected, the first pin
Pipe is connected with the LFD nucleic acid test strip of the first cavity, when the punctures of twin flue syringe needle reacts the LFD in amplification pond
After sealing lid, opening check valve, buffering agents flows into reaction amplification pond along the second needle tubing, and is utilized rainbow by the first needle tubing
Suction principle is drawn on LFD nucleic acid test strip and realizes the reaction of LAMP-LFD quick visualization.
Detection chip device the most according to claim 1, it is characterised in that the receipts that are mixed described in: sample pond is by disk
Upper cover and the discoidal base of shape involute, and the first capillary channel is arranged on and covers, one end of the first capillary channel
Being connected with mixing homogeneously groove, the other end is connected with sample adding device, and mix homogeneously groove is arranged on base, mix homogeneously groove
If the segmentation annulus concentric by dried layer forms, segmentation annulus is provided with the breach flowing to water jacket for feed liquid from inside groove, point
Join mouth to be radially uniformly arranged on outermost segmentation annulus.
Detection chip device the most according to claim 2, it is characterised in that: described segmentation annulus is three layers, lacks
Mouth is 3～4 arc notch, and arc notch is equidirectional being disposed in an evenly spaced relation in deviously on segmentation annulus, in distribution openings
The inwall of the outermost segmentation annulus of side is provided with the accommodating groove of parabolic shape, and the bottom of accommodating groove is provided with pore, carefully
Hole is connected by the interface of distribution openings with chip reaction box.
Detection chip device the most according to claim 1, it is characterised in that: described chip reaction box includes one or two
The box body of end opening, one end of box body is provided with the adapter of the second capillary channel in removably sealing shroud is provided with one, and interface sets
Putting the end in adapter, reaction amplification pond removably sealing shroud is located at the other end of box body, and the front end in reaction amplification pond sets
Have the capillary channel interface being connected with the second capillary channel, be additionally provided with in box body with react amplification pond be connected for
The buffering release pond of additional air backflow.
Detection chip device the most according to claim 4, it is characterised in that: described reaction amplification pond includes cylinder
Shape shell, caves inward into cylindrical cavity bottom cylinder blanket, capillary channel interface is arranged on housing forward end and cylinder
Shape cavity is connected, and probe is arranged on cylindrical empty intracavity, and it is airtight thin material that LFD seals lid, and LFD seals lid
Upper end is provided with the draw-in groove can being tightly connected after being inserted by the twin flue syringe needle of LFD reaction box.
Detection chip device the most according to claim 4, it is characterised in that: the lower end in described buffering release pond with
The capillary channel interface in reaction amplification pond is connected, and the upper end in buffering release pond is provided with the sealing lid of an elasticity, this sealing lid
Recessed state is presented when non-airtight sample-adding.
7. according to the detection chip device described in claim 1 to 6 any claim, it is characterised in that: described LFD
On the housing of reaction box, top or side corresponding to the second cavity are provided with check valve switch.
8. according to the detection chip device described in claim 1 to 6 any claim, it is characterised in that: described point
Joining mouth is evenly spaced 12, and described chip reaction box is corresponding 12.
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| CN201410060517.XA CN104862216B (en) | 2014-02-21 | 2014-02-21 | High flux visualization total enclosing split type LAMP-LFD detection chip device |
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| CN201410060517.XA CN104862216B (en) | 2014-02-21 | 2014-02-21 | High flux visualization total enclosing split type LAMP-LFD detection chip device |
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| CN104789448B (en) * | 2015-04-16 | 2017-11-03 | 青海省畜牧兽医科学院 | LAMP sample-adding reaction vessel and its application method |
| CN109387628A (en) * | 2016-03-14 | 2019-02-26 | 北京康华源科技发展有限公司 | It is centrifugated detection method |
| CN107988046B (en) * | 2018-01-23 | 2021-02-19 | 吉林大学 | LAMP-based self-suction type multi-channel pathogen detection micro-fluidic chip |
| CN108642146B (en) * | 2018-06-01 | 2024-02-02 | 广州双螺旋基因技术有限公司 | S-type microtube nucleic acid amplification closed reaction tube capable of being preloaded with reagent |
| CN111733288B (en) * | 2020-06-22 | 2022-09-30 | 厦门大学 | Nucleic acid detection method and device and application in COVID-19 detection |
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| DE3939024A1 (en) * | 1989-05-17 | 1990-11-22 | Huelsta Werke Huels Kg | SPRING BAR FOR A LATTENED GRID OR THE LIKE |
| US6598885B2 (en) * | 2001-10-23 | 2003-07-29 | Liquidspring Technologies, Inc. | Single valve control of damping and stiffness in a liquid spring system |
| CN1888902B (en) * | 2006-08-11 | 2011-05-18 | 杭州优思达生物技术有限公司 | Full closed target nucleic amplifier fast testing device |
| US8922198B2 (en) * | 2010-10-26 | 2014-12-30 | Blackberry Limited | System and method for calibrating a magnetometer according to a quality threshold |
| CN102199531A (en) * | 2011-03-30 | 2011-09-28 | 复旦大学 | Microfluidic chip for multiple loop-mediated isothermal amplification (LAMP) detection and preparation method thereof |
| CN202808796U (en) * | 2012-07-31 | 2013-03-20 | 中国检验检疫科学研究院 | Loop-mediated isothermal amplification reaction tube |
| CN203728843U (en) * | 2014-02-21 | 2014-07-23 | 宁波大学 | Split type LAMP-LFD (loop-mediated isothermal amplification-lateral flow dipstick) chip detection device with characteristics of high throughput, visualization and full closing |
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