CN207472771U - A kind of separation preserves reagent filtering detection device - Google Patents

Abstract

A kind of separation preserves reagent filtering detection device, including seal cap, filter pump head and filtering reaction tube, seal cap is compressible, include first kind reagent, filtering reaction tube is located in filtering pump head, filtering reaction bottom of the tube is equipped with sealing-plug, the filter membrane that can retain microorganism is equipped in filtering reaction tube, filtering reaction tube upper end is connected by support ring with seal cap, support ring bottom is equipped with the sealing isolation layer for placing the second class reagent, hollow sealing bar is located in support ring and seal cap, sealing bar upper end is equipped with the head cover of sealant, the sharp protrusion for puncturing sealant is convexly equipped on head cover, seal cap top inner wall is equipped with up-side down triangle protrusion, sealing bar bottom is equipped with the protrusion for puncturing separation layer.The utility model is rational in infrastructure ingenious, easy to operate, is incubated and operates without full shading, just directly can carry out accurate quantification to the object content to be checked in liquid, simplify detection process, save detection time and cost, has the characteristics that detect sensitive, fast, accurately.

Description

A kind of separation preserves reagent filtering detection device

Technical field

The utility model belongs to technical field of microbial detection, and a kind of separation preserves agent detection devices, more particularly, to The microorganism of a kind of organic and water environment microorganism is extracted and device for fast detecting.

Background technology

Content of microorganisms test has become what municipal water use, food and beverage, clinic, cosmetics and pharmaceutical field were faced One challenge.However China is in research of the fields such as food, drug, pesticide, soil, environment to microbial contamination and related mark Accurate and method is more, and the microbial contamination in fuel and fuel system still belongs to new field in China, in oil product industry category Newer things, the also all external imports of used detection device, method are with high costs.

On the other hand, although China's quality standard of water environment (GB3838-2002) defines 24 elementary item standard limits It is worth (23 physical and chemical indexes and 1 pathogenic microorganism index), but current China's water quality assessment is very examined less still based on physical and chemical index Consider pathogenic microorganism index.Existing research (Yang Yong etc., 2013) shows the major water system in China by different degrees of cause of disease It is also pathogenic microorganism institute that microbial contamination, external 40% river and the water quality in river mouth, which are difficult to reach quality standard of water environment, It causes, if considering pathogenic microorganism index, then the water grade in China major part river will be further compromised.Water body disease Pathogenic microorganism pollution constitutes human health huge potential threat.And with culture fishery scale it is continuous expand and The pollution of the continuous improvement of intensive degree, sea water culture environment and breeding water body increasingly sharpens, aquatic animal disease outburst day Become frequent and severe, huge economic loss is caused to aquaculture industry.Water body microbiological contamination and its control increasingly obtain To the attention of people.Therefore it is realized using effective ways and means extremely urgent to the real time monitoring of water environment and early warning.

Content of microorganisms test (being also known as microbial limit test) is intended to determine in finished product or non-sterilized raw material The number of the aerobe of survival.Content of microorganisms test is divided into two parts progress：First part is quantitative stage (microorganism meter Number), in this stage, determine the sum of aerobe and saccharomycete and the sum of mould.Second part is qualitative stage (microorganism that test is specified), it is intended to determine that specified microorganisms whether there is.

Through looking into, the Chinese patent of existing Patent No. CN201621066914.9《A kind of liquid state filtering extraction microorganism Detection device》, including extraction element and reaction unit, wherein extraction element includes screen pipe and filtering pump head, the bottom of screen pipe Portion is equipped with the filter membrane that can retain microorganism, and the upper end of screen pipe is connected with the container equipped with liquid sample to be checked by taking over, filtered Pump head is connected to the lower end of screen pipe, and filtering pump head lower end is connected by constant displacement pump, liquid suction pipe with hydrops bottle；Reaction unit packet Include reaction tube, above-mentioned retention has the screen pipe of microorganism and containing the seal cap for detecting liquid, screen pipe is placed in reaction tube, close Sealing cap is connected by outer urgent formula lock with the upper end of reaction tube, during reaction, is made to contain in seal cap and is detected liquid and all flowed to In chimney filter, the filter membrane for having microorganism by retention makes sampling fully be reacted with detection liquid, and reaction unit is put into ATP fluoroscopic examinations It is detected in instrument.Microorganism in liquid can be extracted and be detected to this detection device, and it is accurately fixed to carry out DNA in application fluorescent dye During amount, the operation being incubated with full shading is needed, process is comparatively laborious, it is impossible to directly the content of microorganisms in liquid be carried out accurate Quantitative detection.

Utility model content

Technical problem to be solved in the utility model is to provide a kind of structure design for the above-mentioned state of the art and close It manages ingenious, easy to operate separation and preserves reagent filtering detection device, all kinds of detections can be coordinated to shine the use of shape instrument, it will be to be checked After liquid directly extracts microorganism by present apparatus quantitative filtering, without specially treated, so that it may to be checked in direct quantitative liquid Object content.

Technical solution is used by the utility model solves above-mentioned technical problem：A kind of separation preserves reagent filtering detection Device, including seal cap, filtering pump head and filtering reaction tube, filtering pump head lower end is connected by liquid suction pipe with hydrops bottle, It is characterized in that：The seal cap is compressible seal cap, is arranged in seal cap containing first kind reagent, filtering reaction tube It filters in pump head, the sealed bottom for filtering reaction tube is equipped with a removable lining sealing-plug, filters in reaction tube close to the position of bottom The filter membrane of microbiological materials can be retained by installing, and the upper end for filtering reaction tube is connected by support ring with seal cap, support Bottom is equipped with the sealing isolation layer for placing the second class reagent in ring, and a hollow sealing bar is arranged on support ring and seal cap Interior, the lower seal of sealing bar is plugged on the top for being located at sealing isolation layer in support ring, is equipped at the upper end opening of sealing bar One head cover, the top of head cover are equipped with sealant, the sharp protrusion for puncturing sealant are at least convexly equipped on head cover, sealing bar Bottom form for puncture support ring sealing isolation layer protrusion, when seal cap is compressed downwards, the point at least on head cover Sharp protrusion punctures sealant, and sealing bar is pushed to move down the sealing isolation layer for puncturing support ring, first kind reagent outflow and and the The object to be checked for flowing into after the mixing of two class reagents in filtering reaction tube and being acquired on filter membrane reacts, convenient for finally by instrument Sensor reads related data.

Further, the top inner wall of the seal cap also is provided with up-side down triangle protrusion, makes the up-side down triangle in seal cap convex It rises and punctures sealant with the sharp protrusion cooperation on head cover.

As an improvement, the lower part of the seal cap is the internal thread linkage section being connected with support ring, the top of seal cap It it is compressible section, the top of seal cap can realize compression downwards by the control of instrument button；Head cover is located at close in ring hat shape lid The top of bar is sealed, there is the through hole with the centre bore coaxial communication of sealing bar on head cover, sealant is bonded close using aluminum tissue paper It is enclosed in the upper opening of head cover.

As an improvement, the top of the sealing bar is cylinder, the circular cone of downward flaring diameter is shaped in the middle part of sealing bar Type, the middle part of sealing bar are tightly connected using female Luer mode and support ring, the protrusion of sealing bar bottom for trigone shape or Rotate the edge of a knife.

As an improvement, being tried convenient for the first kind for a plurality of rule is equipped in taper terminal in the middle part of the pyramid type of the sealing bar The sunken inside rain line that agent flows downward.

It improves again, the support ring includes the support outer shroud being vertically connected with and support inner ring, wherein the upper end of support outer shroud It is connected with seal cap, the lower end of inner ring is supported to be connected with filtering reaction tube, the top for supporting outer shroud is the circle with sealing bar The matched female Luer of tapered middle supports the lower part of outer shroud to be equipped with the stop bit concave bar for being used for sealing bar clamping, supports inner ring For the structure that top bore is big, lower part bore is small, the bottom of inner ring is supported to be equipped with for the center empty slot of the second class reagent of placement, Sealing isolation layer is upper and lower two layers, is sealingly disposed in the both sides up and down of center empty slot respectively, and sealing isolation layer is close using aluminium-foil paper Envelope.

It improves again, the lower outside circumferential direction of the support outer shroud is equipped with raised screens muscle, supports in the upper inside wall of inner ring Circumferential direction is equipped with corresponding concave card muscle slot, and the lower part of outer shroud is supported to be plugged in support inner ring and is offseted with step surface, the two passes through Raised screens muscle is connect with the fitted seal of concave card muscle slot.

It improves again, in the inner cavity of the support inner ring, the top of sealing isolation layer is equipped with to support sealing bar to increase The spring of leakproofness, spring are divided into high-order spring and two, low level spring, when sealing bar is compressed downwards under the compression of seal cap High-order spring to low level spring stress point when, the first kind reagent in seal cap is flowed into support ring bottom and the second class reagent It mixes, after sealing bar continues to move down and make the sealing isolation layer that low level spring stress punctures support inner ring bottom again, mixing Reagent flow into filtering reaction tube in.

Further, the high-order spring is high-order low force spring, and low level spring is gravity spring.

Further, the lower outside circumferential direction of the support inner ring is convexly equipped with screens muscle, filters on the upper end inner wall of reaction tube Corresponding circumferential groove is formed, the lower part of inner ring is supported to be plugged on matching by screens muscle and circumferential groove in filtering reaction tube Connection is closed to fix.

Further, the filter membrane fixes support chip by threaded position-arresting ring with quartz sand, metal mesh sheet is located in The bottom of reaction tube is filtered, the step that positioning is planted for filtering reaction tube is equipped in filtering pump head, filters the upper and lower ends of pump head External screw thread is separately formed, the lower end for filtering pump head is connected by liquid suction pipe with hydrops bottle, filters upper end and the screw thread of pump head It is connected after sealing cover connection by pipe with open bottles.

Finally, the second class reagent is liquid or solid.

Compared with prior art, the utility model has the advantage of：Other than first kind reagent is set in seal cap, Support ring bottom also sets up the second class reagent, can coordinate all kinds of detections shine shape instrument use, liquid to be checked is directly passed through After present apparatus quantitative filtering lifts microorganism, without specially treated, so that it may the object content to be checked in direct quantitative liquid, particularly DNA accurate quantifications are carried out using fluorescent dye, DNA and fluorescent dye Two Areas can be individually positioned in, then be placed in instrument In, incubations is protected from light by button realization, can remove the operation of full shading incubation from this way, the object to be checked in direct quantitative liquid contains Amount；Seal cap be designed to it is compressible, in this way by compression can sealing bar push, convenient for puncture sealant cause two classes try Agent is flowed out and is mixed；Design integrates the filtering reaction tube of reaction and filtering, enormously simplifies structure.The utility model structure Reasonable design is ingenious, easy to operate, without the operation that full shading is incubated, just directly can carry out essence to the object content to be checked in liquid Determine amount, enormously simplify detection process, saved detection time and cost, have the characteristics that detect it is sensitive, fast, accurately, The utility model establishes fast microbiological content measuring platform, substantially reduces the time obtained needed for result, so as to Rapid take corrective action efficiently solves microbial contamination test problems in liquid.

Description of the drawings

Fig. 1 is the structure diagram of the utility model；

Fig. 2 is the sectional view of Fig. 1；

Fig. 3 is the exploded view of Fig. 1.

Specific embodiment

The utility model is described in further detail below in conjunction with attached drawing embodiment.

As shown in Figures 1 to 3, a kind of separation preserves reagent filtering detection device, including seal cap 1, sealing bar 5, support ring 4th, pump head 2 and filtering reaction tube 3 are filtered, wherein filtering reaction tube 3 is arranged in filtering pump head 2, filters the bottom of reaction tube 3 Sealing is equipped with a removable lining sealing-plug 9, filters the filter membrane for being equipped in reaction tube 3 close to the position of bottom and can retaining microorganism 31, the upper end of filtering reaction tube 3 is connected by support ring 4 with seal cap 1, and bottom is equipped with to place second in support ring 4 The sealing isolation layer 422 of class reagent 8, seal cap 1 are compressible seal cap 1, and the lower part of seal cap 1 is is connected with support ring 4 The internal thread linkage section connect, the top of seal cap 1 are compressible section, containing first kind reagent in seal cap 1, seal cap 1 it is upper Portion can realize compression downwards by the control of instrument button；Sealing bar 5 is hollow structure, is arranged on support ring 4 and seal cap 1 Interior, the lower seal of sealing bar 5 is plugged on the top for being located at sealing isolation layer 422 in support ring 4, the upper end opening of sealing bar 5 Place is equipped with a head cover 6, and the upper end periphery circumferential direction of sealing bar 5 is equipped with the convex ribs 52 being connected with head cover 6, and head cover 6 is in ring hat shape lid The top of sealing bar 5 is located at, there is the through hole with the centre bore coaxial communication of sealing bar 5, the top of head cover 6 is set on head cover 6 There is a sealant 61, sealant 61 is bonded and sealed the projection in the upper opening of head cover 6,6 opening inboard of head cover using aluminum tissue paper It is useful for puncturing the sharp protrusion 62 of sealant 61, the top inner wall of seal cap 1 is equipped with up-side down triangle protrusion, due to seal cap 1 Be built-in with first kind reagent, first kind reagent is liquid reagent, due to the effect of atmospheric pressure, below air pressure can be more than seal cap 1 The inside pressure causes the inside liquid that cannot smoothly flow out, if it is desired that the inside liquid can be smoothly flowed in following container, needs Upper and lower air pressure is consistent, that is, puncturing 61 first kind reagent of sealant of head cover 6 could flow out, and the bottom of sealing bar 5 forms Protrusion 51, protrusion 51 are typically irregular, and protrusion 51 can be prismatic shape or the rotation edge of a knife, for puncturing the close of support ring 4 Absciss layer 422 is insulated, and makes to form irregular status in place of rupture, is flowed out convenient for liquid；When seal cap 1 is compressed downwards, sealing Up-side down triangle protrusion in cap 1 punctures sealant 61, and sealing bar 5 is pushed to move down and punctures branch with the sharp protrusion 62 on head cover 6 The sealing isolation layer 422 of pushing out ring 4, the outflow of first kind reagent simultaneously and after the mixing of the second class reagent 8 flows into filtering reaction tube 3 and mistake The object to be checked acquired on filter membrane 31 reacts, and related data is read finally by Instrument sensor；The top of sealing bar 5 is circle Cylindricality, the middle part of sealing bar 5 are shaped to the pyramid type of downward flaring diameter, and the middle part of sealing bar 5 is using female Luer mode and branch Pushing out ring 4 is tightly connected, and the downward convenient for first kind reagent of a plurality of rule is equipped in taper terminal in the middle part of the pyramid type of sealing bar 5 The sunken inside rain line of flowing, is exactly similar to indent fluid slot；Support ring 4 includes the support outer shroud 41 being vertically connected with and support inner ring 42, wherein the upper end of support outer shroud 41 is connected with seal cap 1, the lower end of inner ring 42 is supported to be connected with filtering reaction tube 3, is propped up The upper outside of support outer shroud 41 forms the external screw thread 412 being threadedly coupled with seal cap 1, support the upper inner of outer shroud 41 for The matched female Luer in cone middle part of sealing bar 5, the lower part of support outer shroud 41 are equipped with the stop bit for 5 clamping of sealing bar Concave bar, support inner ring 42 are the structure that top bore is big, lower part bore is small, and the bottom of support inner ring 42 is equipped with to place second The center empty slot 421 of class reagent 8, the second class reagent 8 can be liquid or solid, and sealing isolation layer 422 is upper and lower two layers, respectively The both sides up and down of center empty slot 421 are sealingly disposed in, sealing isolation layer 422 is sealed using aluminium-foil paper, supports the lower part of outer shroud 41 Outside circumferential direction is equipped with raised screens muscle 411, and circumferential direction in the upper inside wall of inner ring 42 is supported to be equipped with corresponding concave card muscle slot 423, The lower part of support outer shroud 41 is plugged in support inner ring 42 and offsets with step surface, and the two passes through raised screens muscle 411 and concave card The fitted seal connection of muscle slot 423；It supports in the inner cavity of inner ring 42, the top of sealing isolation layer 422 is equipped with to support sealing Bar 5 is to increase the spring 7 of leakproofness, and spring 7 can be divided into high-order spring and two, low level spring (drawing does not embody), wherein high Position spring is high-order low force spring, and low level spring is gravity spring, and the present embodiment uses a spring, when sealing bar 5 is close When stress point of the high-order spring to low level spring is compressed under the compression of sealing cap 1 downwards, the sealant 61 of head cover 6 and sealing above Separation layer 422 is punctured, and the first kind reagent in seal cap 1 is flowed into 4 bottom of support ring and the second class reagent 8 mixes, close After envelope bar 5 continues to move down and make the sealing isolation layer 422 that low level spring stress punctures 42 bottom of support inner ring again, the examination of mixing Agent is flowed into filtering reaction tube 3, and the lower outside circumferential direction of support inner ring 42 is convexly equipped with screens muscle 424, filters the upper end of reaction tube 3 Corresponding circumferential groove 32 is formed on inner wall, the lower part of support inner ring 42 is plugged in filtering reaction tube 3 through screens muscle 424 With being fixedly connected for circumferential groove 32, filter membrane 31 fixes support chip, metal by threaded position-arresting ring 33 and quartz sand Mesh sheet is located in the bottom of filtering reaction tube 3, and the step that positioning is planted for 3 bottom of filtering reaction tube, mistake are equipped in filtering pump head 1 The upper and lower ends of filter pump head 1 have been separately formed external screw thread, and the lower end of filtering pump head 1 is connected by liquid suction pipe with hydrops bottle, mistake The upper end of filter pump head 1 is connected after being connect with thread sealed closure by pipe with open bottles.

Seal cap 1 and filtering reaction tube 3 in the present embodiment can be split, and individually be preserved.

In use, first kind reagent and the second class reagent 8 first are added in seal cap 1 and 4 bottom of support ring, it then will sealing Cap 1, sealing bar 5, head cover 6 and support ring 4 link into an integrated entity；The filtering reaction tube 3 not sealed is put into filtering pump head 2, button Tight thread sealed closure, is connected in open bottles, and the lower end of filtering pump head 2 is connected to hydrops bottle, and pass through constant displacement pump by pipe Liquid sample to be checked is added dropwise in filtering reaction tube 3, the microorganism in liquid is trapped within the filter membrane 31 in filtering reaction tube 3 On, then sealing cover 9 is connect with filtering the bottom thread of reaction tube 3, by above-mentioned mounted support ring 4 and filtering reaction tube 3 upper end connection sealing, is placed in instrument, pushes compression seal cap 1 by instrument button, puncture the sealant 61 of head cover 6, And sealing bar 5 is pushed to move down, then puncture the upper strata sealing isolation layer 422 of support ring 5, the outflow of first kind reagent and and the second class examination Agent 8 mixes, and sealing bar 5 continues to move down, and punctures lower floor's sealing isolation layer 422 of support ring 5, and mix reagent flows into filtering reaction tube It reacts in 3 with object to be checked, related data is read finally by Instrument sensor.

When application fluorescent dye progress DNA accurate quantifications, the operation used and be incubated with full shading can be removed from, it can be by DNA (i.e. first kind reagent) and fluorescent dye (i.e. the second class reagent) are individually positioned in Two Areas (seal cap 1 and support ring 4), so It is placed in instrument afterwards, incubation is protected from light by button realization, object content to be checked that can be in direct quantitative liquid.

The above is only the preferred embodiment of the utility model, it is noted that for the common skill of the art For art personnel, without departing from the technical principles of the invention, several improvements and modifications can also be made, these improve and Retouching also should be regarded as protection scope of the present invention.

Claims (12)

1. a kind of separation preserves reagent filtering detection device, including seal cap, filtering pump head and filtering reaction tube, pump head is filtered Lower end is connected by liquid suction pipe with hydrops bottle, it is characterised in that：The seal cap is compressible seal cap, and seal cap includes There is first kind reagent, filtering reaction tube is arranged in filtering pump head, and the sealed bottom for filtering reaction tube is removable lining close equipped with one Blocking filters the filter membrane for being equipped in reaction tube close to the position of bottom and can retaining microbiological materials, filters the upper end of reaction tube It is connected by support ring with seal cap, bottom is equipped with the sealing isolation layer for placing the second class reagent in support ring, in one Empty sealing bar is arranged on support ring and in seal cap, the lower seal of sealing bar is plugged in support ring positioned at sealing isolation layer Top, a head cover is equipped at the upper end opening of sealing bar, the top of head cover is equipped with sealant, is at least convexly equipped with and is used on head cover Puncture the sharp protrusion of sealant, the bottom of sealing bar form for puncture support ring sealing isolation layer protrusion, when close When sealing cap compresses downwards, the sharp protrusion at least on head cover punctures sealant, and sealing bar is pushed to move down and punctures the close of support ring Absciss layer is insulated, the outflow of first kind reagent is flowed into filtering reaction tube after simultaneously being mixed with the second class reagent and treated with what is acquired on filter membrane Inspection object reacts, convenient for reading related data finally by Instrument sensor.

2. separation according to claim 1 preserves reagent filtering detection device, it is characterised in that：The top of the seal cap Inner wall also is provided with up-side down triangle protrusion, and the up-side down triangle protrusion in seal cap is made to puncture sealing with the sharp protrusion cooperation on head cover Layer.

3. separation according to claim 1 preserves reagent filtering detection device, it is characterised in that：The lower part of the seal cap For the internal thread linkage section being connected with support ring, the top of seal cap is compressible section, and the top of seal cap can pass through instrument Compression downwards is realized in the control of button；Head cover is located at the top of sealing bar in ring hat shape lid, have on head cover in sealing bar The through hole of heart hole coaxial communication, sealant are bonded and sealed using aluminum tissue paper in the upper opening of head cover.

4. separation according to claim 3 preserves reagent filtering detection device, it is characterised in that：The top of the sealing bar For cylinder, be shaped to the pyramid type of downward flaring diameter in the middle part of sealing bar, the middle part of sealing bar using female Luer mode with Support ring is tightly connected, and the protrusion of sealing bar bottom is trigone shape or the rotation edge of a knife.

5. separation according to claim 4 preserves reagent filtering detection device, it is characterised in that：The circular cone of the sealing bar The sunken inside rain line to flow downward convenient for first kind reagent of a plurality of rule is equipped in the middle part of type in taper terminal.

6. separation according to claim 4 preserves reagent filtering detection device, it is characterised in that：The support ring includes upper The support outer shroud of lower connection and support inner ring, wherein the upper end of support outer shroud is connected with seal cap, support inner ring lower end and Filtering reaction tube is connected, and the top for supporting outer shroud is the matched female Luer in cone middle part with sealing bar, and support is outer The lower part of ring is equipped with the stop bit concave bar for sealing bar clamping, supports inner ring as the structure that top bore is big, lower part bore is small, branch The bottom for supportting inner ring is equipped with for the center empty slot of the second class reagent of placement, and sealing isolation layer is upper and lower two layers, and sealing respectively is set It puts in the both sides up and down of center empty slot, sealing isolation layer is sealed using aluminium-foil paper.

7. separation according to claim 6 preserves reagent filtering detection device, it is characterised in that：Under the support outer shroud It is circumferential on the outside of portion to be equipped with raised screens muscle, circumferential direction in the upper inside wall of inner ring is supported to be equipped with corresponding concave card muscle slot, support is outer The lower part of ring is plugged in support inner ring and offsets with step surface, the fitted seal that the two passes through raised screens muscle and concave card muscle slot Connection.

8. separation according to claim 6 preserves reagent filtering detection device, it is characterised in that：It is described to support the interior of inner ring In chamber, the top of sealing isolation layer be equipped with for supporting sealing bar to increase the spring of leakproofness, spring be divided into high-order spring and Two, low level spring, it is close when sealing bar compresses downwards stress point of the high-order spring to low level spring under the compression of seal cap First kind reagent in sealing cap is flowed into support ring bottom and the second class reagent mixes, and sealing bar continues to move down and makes low level bullet After spring stress punctures the sealing isolation layer of support inner ring bottom again, the reagent of mixing is flowed into filtering reaction tube.

9. separation according to claim 8 preserves reagent filtering detection device, it is characterised in that：The high position spring is height Position low force spring, low level spring are gravity spring.

10. separation according to claim 6 preserves reagent filtering detection device, it is characterised in that：The support inner ring Lower outside circumferential direction is convexly equipped with screens muscle, filters and corresponding circumferential groove is formed on the upper end inner wall of reaction tube, supports inner ring Lower part be plugged on being fixedly connected by screens muscle and circumferential groove in filtering reaction tube.

11. the separation according to claims 1 to 10 any claim preserves reagent filtering detection device, feature exists In：The filter membrane fixes support chip by threaded position-arresting ring and quartz sand, metal mesh sheet is located in the bottom for filtering reaction tube Portion, interior be equipped with of filtering pump head plant the step positioned for filtering reaction tube, and the upper and lower ends for filtering pump head have been separately formed outer spiral shell Line, the lower end for filtering pump head are connected by liquid suction pipe with hydrops bottle, filter pump head upper end connect with thread sealed closure after lead to Pipe is crossed with open bottles to be connected.

12. the separation according to claims 1 to 10 any claim preserves reagent filtering detection device, feature exists In：The second class reagent is liquid or solid.