CN206095753U - Little biological detection device is drawed in liquid type of filtration - Google Patents

Abstract

The utility model provides a little biological detection device is drawed in liquid type of filtration, includes extraction element and reaction unit, and wherein the extraction element includes the filter tube and filters the pump head, and the bottom of filter tube is equipped with the filter membrane that can hold back the microorganism, and the upper end of filter tube waits that the container of examining liquid appearance is connected through taking over with being equipped with, filters the pump head and connects the lower extreme at the filter tube, filtration pump head lower extreme is connected with the hydrops bottle through constant delivery pump, liquid suction pipe, reaction unit includes that reaction tube, the aforesaid hold back the filter tube of microorganism and contain the sealed cap that detects liquid, and in the reaction tube was placed in to the filter tube, the sealed cap was connected through outer upper end of compeling formula hasp and reaction tube, during the reaction, in making in the sealed cap that institute's liquid that detects greatly is whole and flowing the filter tube, makes sampling and measuring liquid fully react through the filter membrane of holding back the microorganism, puts into ATP fluorescence detector with reaction unit and detects. The utility model discloses microbial contamination detects the problem in effectively having solved liquid, has detectivity height, high, the fast characteristics of response time of accuracy.

Description

A kind of liquid state is filtered and extracts microbial detection device

Technical field

The utility model belongs to technical field of microbial detection, is related to a kind of liquid state and filters extraction microorganism detection dress Put, extract and device for fast detecting more particularly, to the microorganism of a kind of organic and water environment microorganism.

Background technology

With the fast development of automobile, aviation and shipbuilding industry, Fuel Dosage is increasing, during storage and use, The problems such as blocking filter and transfer pipeline occurs in fuel oil microorganism pollution is had been reported that both at home and abroad, wherein in Aviation Fuel system The more problems of microorganism pollution, such as filter, air exhauster failure, fuel tank heavy corrosion, fuel gauge probe failure and other instrument Table, nozzle and control element etc. break down, and have a substantial connection with the pollutant that gathers in fuel system, and after further study Show, these pollutants be by the microorganism groups such as mould, saccharomycete, bacterium into.This pollution problem caused by microorganism, Aircraft engine flame-out in flight, wing tank Housing failure etc. can be caused, huge economic loss is not only caused but also produced tight The safety consequences of weight.Accordingly, it would be desirable to the microorganism in fuel system is monitored, detects, controls and is prevented, reduce it and cause Loss.

But research and relevant mark of the China in fields such as food, medicine, agricultural chemicals, soil, environment to microorganism pollution Accurate and method is more, and the microorganism pollution in fuel and fuel system still belongs to new field in China, in oil product industry category Newer things, the testing equipment for being adopted, method also all external imports are with high costs.

On the other hand, although China's quality standard of water environment (GB3838-2002) defines 24 elementary item standard limits Value (23 physical and chemical indexs and 1 pathogenic microorganism index), but the water quality assessment of current China's water is still based on physical and chemical index, it is very few Consider pathogenic microorganism index. studied (Yang Yong etc., 2013) shows, each big water system of China be subject to different degrees of disease It is also pathogenic microorganism institute that pathogenic microorganism pollution, external 40% river and the water quality in river mouth are difficult to reach quality standard of water environment If caused. consider pathogenic microorganism index, then the water grade in China's major part river will be further compromised. water body disease Pathogenic microorganism pollution constitutes huge potential threat to human health.And the continuous expansion with culture fishery scale and The pollution of the continuous improvement of intensive degree, sea water culture environment and breeding water body increasingly sharpens, aquatic animal disease outburst day Become frequent and severe, to aquaculture industry huge economic loss is caused.Water body microbiological contamination and its control are increasingly obtained To the attention of people. realize that the monitor in real time to water environment and early warning are extremely urgent hence with effective ways and means.

This liquid state filters extraction microbial detection device can coordinate what ATP bioluminescence detectors were used, have The advantages of easy to operate, rapid sensitive, with low cost, environmental protection, microbes contamination can be quickly recognized, it is rapid to judge Whether microorganism is exceeded.But ATP fluorescent quick detection devices in the market are for field of detection of food safety and are Surface of solids pollution detection, there is no the device of the detection for organic and water body environment microbes contamination.

The content of the invention

Technical problem to be solved in the utility model is to provide a kind of liquid state for the above-mentioned state of the art to filter Microbial detection device is extracted, microorganism pollution test problems in liquid can be efficiently solved, it is quick with easy to operate, detection Sensitive the characteristics of.

The utility model solve the technical scheme that adopted of above-mentioned technical problem for：A kind of liquid state is filtered and extracts microorganism Detection means, it is characterised in that including extraction element and the part of reaction unit two, wherein extraction element includes screen pipe, filter pump Head and constant displacement pump, the bottom of screen pipe is provided with the filter membrane that can retain microorganism, the upper end of screen pipe by adapter be equipped with it is to be checked The container of liquid sample is connected, and filters the lower end that pump head is connected to screen pipe, filters pump head lower end and connects liquid suction pipe by constant displacement pump, Finally it is connected with hydrops bottle；

Reaction unit includes that one there is the reaction tube of lucifuge setting, above-mentioned retention to have the screen pipe of microorganism and containing inspection The seal cap of liquid is surveyed, screen pipe is placed in reaction tube, seal cap is connected by outer urgent formula lock with the upper end of reaction tube, is reacted When, make to be contained in seal cap detection liquid whole stream in screen pipe, through retaining the filter membrane for having microorganism sampling is made with detection liquid Fully reaction, reaction unit is put in ATP fluorescence detectors and is detected.

Used as improvement, the cone structure of the bottom in undergauge of the screen pipe, filter membrane is hydrophilic water-oil separationg film, if Put in the top of cone structure.

Used as improvement, the upper shed of the screen pipe is covered with a closure, takes over and is managed for disposable, and one end of adapter is inserted Put in container, the other end is connected with seal cap sealing.

Improve again, seal bar is provided with the seal cap, seal bar is broken hollow tube, the detection in seal cap Liquid is flowed in screen pipe by the seal bar that fractures, crush seal cap.

Improve again, shading pipe is arranged with outside the reaction tube.

Compared with prior art, the utility model has the advantage of：The water-oil separationg film of special substance is set in screen pipe, Can be used to retain microorganism, filter pump head connection constant displacement pump, can quantitative filtering liquid sample to be checked, reaction tube arranged using shading, kept away Exempt from light to detecting the impact of liquid.The utility model is rational in infrastructure, easy to operate, and ATP fluorescence detectors can be coordinated to use, and will treat Inspection liquid is directly lifted after microorganism by this device quantitative filtering, without the need for specially treated, just can be in direct quantitative liquid it is micro- Biological content, efficiently solves microorganism pollution test problems in liquid, with when detection sensitivity height, high accuracy, response Between it is fast the characteristics of.

Description of the drawings

Fig. 1 is the structural representation of extraction element in the utility model；

Fig. 2 is the structural representation for filtering pump head；

Fig. 3 is the structural representation of screen pipe；

Fig. 4 is the structure decomposition figure of the utility model detection means.

Specific embodiment

The utility model is described in further detail below in conjunction with accompanying drawing embodiment.

As shown in figures 1-4, a kind of liquid state is filtered and extracts microbial detection device, including extraction element and detection means Two parts, wherein extraction element include screen pipe 1, filter pump head 6 and constant displacement pump, and the taper of the bottom in undergauge of screen pipe 1 is tied Structure, being provided with the bottom of screen pipe 1 can retain the filter membrane 11 of microorganism, and filter membrane 11 is using hydrophilic made by special material Water-oil separationg film, filters the lower end that pump head 6 is arranged on screen pipe 1, filters the lower end of pump head 6 connection constant displacement pump 12, and by drawing liquid Pipe 9 is connected with hydrops bottle 13, and the upper end sealing of screen pipe 1 is covered with a closure 10, and the closure 10 of the present embodiment is logical Cross threaded connection to seal with screen pipe 1, sealing on closure 10 is plugged with an adapter 8, and screen pipe 1 is treated by adapter 8 with being equipped with The container 7 of inspection liquid sample is connected, and container 7 is an open glass bottle, thus liquid sample to be checked is dropped to into filtration by constant displacement pump In pipe 1, the microorganism in liquid is trapped within the filter membrane 11 above screen pipe 1；

Detection means has the screen pipe 1 of microorganism and containing inspection including a reaction tube 2 with lucifuge setting, above-mentioned section Survey outside the seal cap 3 of liquid, reaction tube 2 and be arranged with shading pipe 5, seal cap 3 need to carry out Cord blood, seal cap 3 when unused Interior detection liquid is made up of reactant liquor and lysate, and cracking formula of liquid is N- sodium lauroyl sarcosine 3g/100ml, Tris-Hcl 100mmol/L (pH 8.0), EDTA 50mmol/L (pH 8.0), Nacl 500mmol/L, 20%SDS, mainly for organic In common some fungies, mould etc., using its good biological degradability feature, directly crush fungal cell wall, reactant liquor It is that luciferase and beetle fluorescein are constituted；Screen pipe 1 is placed in reaction tube 2, seal cap 3 by outer urgent formula lock 4 with it is anti- Should the upper end of pipe 2 be tightly connected, is provided with seal bar in seal cap 3, seal bar is broken hollow tube, during reaction, will be close Envelope bar fractures, crush seal cap 3, makes to be contained in seal cap 3 detection liquid whole stream in screen pipe 1, has microorganism through retention Filter membrane 11 sampling is fully reacted with detection liquid, wink gathers the bottom of reaction tube 2 from rear detection liquid by filter membrane 11, rapid general Reaction unit is put in ATP fluorescence detectors, presses OK keys, and reading is carried out after 10s.

Specific operation process is：Pulling down seal cap 3 when unused carries out Cord blood, first extracts sample to be tested, incited somebody to action Chimney filter 1 is put to filtering in pump head 6, buckles thread sealed closure 10, and in being connected to container 7 (open bottles), the lower end for filtering pump head 6 leads to Cross pipe and be connected to hydrops bottle 13, and liquid sample to be checked is dropped in screen pipe 1 by constant displacement pump 12, the microorganism quilt in liquid It is trapped on the filter membrane 11 above screen pipe 1, then screen pipe 1 is put in reaction tube 2, takes out close under the conditions of Cord blood Sealing cap 3 is placed to room temperature (about 30 minutes), and seal cap 3 is connected by outer urgent formula lock 4 with reaction tube 2, and fracture seal bar, Make wherein to contain after crush seal cap 3 and detect that liquid stream enters screen pipe 1, after sampling is fully reacted with detection liquid, wink is from rear detection liquid The bottom of reaction tube 2 is gathered by filter membrane, reaction unit is put in ATP fluorescence detectors rapidly, OK keys are pressed, after 10s Carry out reading.

The above is only preferred embodiment of the present utility model, it is noted that for the common skill of the art For art personnel, on the premise of without departing from the utility model know-why, some improvements and modifications can also be made, these change Enter and retouch also to should be regarded as protection domain of the present utility model.

Claims (5)

1. a kind of liquid state is filtered and extracts microbial detection device, it is characterised in that including extraction element and reaction unit two Point, wherein extraction element includes screen pipe, filters pump head and constant displacement pump, and the bottom of screen pipe is provided with the filter that can retain microorganism Film, the upper end of screen pipe is connected by adapter with the container equipped with liquid sample to be checked, filters the lower end that pump head is connected to screen pipe, Filter pump head lower end and liquid suction pipe is connected by constant displacement pump, be finally connected with hydrops bottle；

Reaction unit includes that one there is the reaction tube of lucifuge setting, above-mentioned retention to have the screen pipe of microorganism and containing detection liquid Seal cap, screen pipe is placed in reaction tube, and seal cap is connected by outer urgent formula lock with the upper end of reaction tube, during reaction, Make to be contained in seal cap detection liquid whole stream in screen pipe, make sampling and detection liquid abundant through retaining the filter membrane for having microorganism Reaction, reaction unit is put in ATP fluorescence detectors and is detected.

2. liquid state according to claim 1 is filtered and extracts microbial detection device, it is characterised in that：The screen pipe Cone structure of the bottom in undergauge, filter membrane is hydrophilic water-oil separationg film, is arranged on the top of cone structure.

3. liquid state according to claim 1 is filtered and extracts microbial detection device, it is characterised in that：The screen pipe Upper shed is covered with a closure, takes over as disposable pipe, and one end of adapter is plugged in container, the other end and seal cap sealing Connection.

4. liquid state according to claim 1 is filtered and extracts microbial detection device, it is characterised in that：In the seal cap Seal bar is provided with, seal bar is broken hollow tube, the detection liquid in seal cap is by the seal bar that fractures, crush seal cap In flowing into screen pipe.

5. the liquid state according to Claims 1-4 any claim is filtered and extracts microbial detection device, and its feature exists In：Shading pipe is arranged with outside the reaction tube.