CN204462153U - Microbial cells microfiltration check-out console - Google Patents
Microbial cells microfiltration check-out console Download PDFInfo
- Publication number
- CN204462153U CN204462153U CN201520091100.XU CN201520091100U CN204462153U CN 204462153 U CN204462153 U CN 204462153U CN 201520091100 U CN201520091100 U CN 201520091100U CN 204462153 U CN204462153 U CN 204462153U
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- China
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- check
- lower floor
- out console
- microbial cells
- hollow cylinder
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Abstract
Microbial cells microfiltration check-out console.Comprise upper strata right cylinder, handle marking plate and lower floor's hollow cylinder, upper strata right cylinder and lower floor's hollow cylinder are threaded connection, liquid feeding pressurization through hole is provided with in the middle of the right cylinder of upper strata, liquid feeding pressurization through hole lower end is provided with two-stage shoulder hole, outer end setting-in filter plate with holes, the resistance to plenum chamber of inner formation; The bottom of lower floor's hollow cylinder is filtered solution funnel, and middle part is that filtrate flow portals, and places miillpore filter above filtered solution funnel.Microbial cells in liquid effectively can be preserved and be trapped on filter membrane by this check-out console, then by the specific detection method of tested substance, obtains qualitative, the quantitative result to detected material.In to liquid in the pathogenic microorganism examination, the pathogen thalline of Ke Dui≤1x10/ml concentration makes qualitative, quantitative detection assay effectively fast; Detecting Microorganism in Water, in liquid food, microbial contamination security detects, and all has extensively actual application value.
Description
Technical field
The utility model belongs to microbial cells detection field, relates to a kind of fast testing plate that can be used for filtering the microbial cells contained in liquid.This check-out console can be widely used in fast qualitative, quantitatively detection assay to the low concentration microbial cells contained in test sample, pathogen thalline (bacterium bulk concentration≤1 x10/ml).Also can be applicable to the fast qualitative, the quantitatively detection assay that Microorganism in Water thalline are detected, liquid food microbial contamination detects.
Background technology
When contained in liquid be that low concentration is (as microbial bacteria bulk concentration ﹤ 1x10
3individual/ml), small particle diameter (particle size is at micron order) such test sample time, detection assay qualitative, quantitative is fast carried out to it, often a very thorny thing.In the past to qualitative, the quantitative measurement of microbial cells, usual way first carries out expansion to it to be separated cultivation, improves unit concentration, then measure.Or adopt ultracentrifugation collect centrifugal thing or first concentrated by its wash-out again by low concentration species adsorbs with adsorption method, then improve concentration and measure.These methods all also exist obvious deficiency, and one is that the time cycle is long, and thalline expands to be separated to cultivate generally to be needed 3-7 days (bacterium be 3 days, fungi be 5-7 days).Two is that using method is complicated, and equipment cost is high.Therefore provide one can carry out filtering, concentrate, detection assay utensil, beyond doubt an optimal solution fast and accurately.Same this desirable filtration, concentrated, device for fast detecting, in the microbial cells detection assay work for low concentrations such as the detection of Microorganism in Water thalline, liquid food microbial contamination detections, also can obtain satisfied result.
Summary of the invention
The purpose of this utility model is for the many deficiencies existing for current existing technology and equipment, adopts the know-why of the inverse application of miillpore filter, provides a kind of practical application equipment solving these deficiencies---
microbial cells microfiltration check-out console.
It is degerming that usual miillpore filter (0.22 micron, aperture) is mainly used in liquid, and (because the individual volume of microbial cells is all greater than 0.22 micron) liquid filters through 0.22 micron membrane filter, by whole for the thalline in liquid filtering, thus ensures the security of product.(as transfusion medicine, cell culture medium etc. all adopt the method for 0.22 micron membrane filter filtration sterilization) and the utility model are through filtering with microporous membrane by the microbial cells of the low concentration (containing dense degree≤1 x10 of bacterium/ml) in testing liquid, make the unknown microbial cells high enrichment in testing sample, and be retained on filter membrane.Then directly to use previously prepared completing, the known specific antibody with clear and definite Pseudomonas attribute, carries out specific combination with it.Simultaneously specific antibody self marker has fluorescence nano quantum dot, amplifies through its signal, thus fast qualitative, the quantitative detection assay of microbial cells in complete paired samples.
The technical solution of the utility model
A kind of microbial cells microfiltration check-out console, this check-out console comprises upper strata right cylinder, handle marking plate and lower floor's hollow cylinder, described handle marking plate and lower floor's hollow cylinder or upper strata right cylinder are connected as a single entity, cylindrical lower end, upper strata is provided with external thread and forms screw bolt, the inside, upper end of lower floor's hollow cylinder is provided with internal thread and forms the screw thread screw socket coordinated with screw bolt, cylindrical centre, upper strata is provided with liquid feeding pressurization through hole, the lower end of liquid feeding pressurization through hole is provided with two-stage shoulder hole, the shoulder hole of outer end is used for setting-in filter plate with holes, inner shoulder hole forms resistance to plenum chamber, the bottom recessed formation filtered solution funnel of lower floor's hollow cylinder, the middle part of filtered solution funnel is that filtrate flow portals, for placing miillpore filter above filtered solution funnel.
The periphery, upper end of the filtered solution funnel bottom described lower floor's hollow cylinder is provided with a withstand voltage aprons slot of annular, is embedded with press mold cushion rubber in aprons slot.
Described filtered solution inner wall of hopper is provided with to heart-shaped chute.
The plane of exterior bottom for ease of placing of described lower floor's hollow cylinder.
The aperture of described miillpore filter is 0.22 micron.
Described handle marking plate is rectangular planes, and rectangular planes both wings can be used as handle and print sample marker code labeling, to the identification detecting thing.
Described check-out console material can be: the macromolecular material materials such as PS material, ABS material, PP material.
This microbial cells microfiltration check-out console, has obvious practical application meaning in low concentration microbial cells detection assay in liquid.Can be widely used in that clinical disease pathogenic microorganism detects fast, the monitoring of Microorganism in Water thalline, liquid food microbial safety detection etc. and people's livelihood association area.
advantage of the present utility model and beneficial effect:
In the detection assay of the utility model low concentration microbial cells in liquid, thalline high enrichment can be made, and be retained on miillpore filter.Amplified by the specific binding of specific antibody and the fluorescence signal of fluorescence nano quantum dot, qualitative and quantitative analysis fast and accurately can be carried out to microbial cells and measure.In fields such as analysis of clinic pathogenic microorganism detection, the detection of Microorganism in Water thalline, the detections of liquid food microbial safety, there is practical application meaning widely.Application of the present utility model can produce good social effect and economic worth surely.
Accompanying drawing explanation
Fig. 1 is the microfiltration check-out console overall appearance schematic diagram of microbial cells;
Fig. 2 is that the microfiltration check-out console of microbial cells splits each position schematic diagram;
In figure: 1 handle marking plate, 2 upper strata right cylinders, 3 liquid feeding pressurization through holes, 4 sample identification codes, 5 lower floor's hollow cylinders, 6 resistance to plenum chambers, 7 screw bolts, 8 filter plates with holes, 9 rib-loop screw sockets, 10 press mold cushion rubbers, 11 miillpore filters, 12 withstand voltage aprons slots, 13 filtered solution funnels, 14 filtrate flows portal.
Embodiment
embodiment 1
as shown in Figure 1 and Figure 2microbial cells microfiltration check-out console
,this check-out console comprises upper strata right cylinder 2, handle marking plate 1 and lower floor's hollow cylinder 5, described handle marking plate and lower floor's hollow cylinder are connected as a single entity, cylindrical lower end, upper strata is provided with external thread and forms screw bolt 7, the inside, upper end of lower floor's hollow cylinder is provided with internal thread and forms the screw thread screw socket 9 coordinated with screw bolt, cylindrical centre, upper strata is provided with liquid feeding pressurization through hole 3, the lower end of liquid feeding pressurization through hole is provided with two-stage shoulder hole, the shoulder hole of outer end is used for setting-in filter plate 8 with holes, and inner shoulder hole forms resistance to plenum chamber 6; The bottom recessed formation filtered solution funnel 13 of lower floor's hollow cylinder, filtered solution inner wall of hopper is provided with to heart-shaped chute () in figure slightly, the middle part of filtered solution funnel is that filtrate flow portals 14, and for placing miillpore filter 11 above filtered solution funnel, the aperture of described miillpore filter is 0.22 micron.The periphery, upper end of described filtered solution funnel is provided with a withstand voltage aprons slot 12 of annular, is embedded with press mold cushion rubber 10 in aprons slot.
The plane of exterior bottom for ease of placing of described lower floor's hollow cylinder.
Described handle marking plate is rectangular planes, and rectangular planes both wings can be used as handle and print sample marker code 4, to the identification detecting thing.
Described check-out console material can be: the macromolecular material materials such as PS material, ABS material, PP material.
The embody rule of check-out console
For the application to clinical urinary system detection of pathogens;
1.. use 2ml injector for medical purpose, draw Urine in Patients sample 1.0ml to be measured.Syringe and the check-out console liquid feeding through hole 3 that pressurizes is connected, by miillpore filter (being less than 0.22 micron) filter urine.Extremely low concentration (≤10/ml can be made) with people's urinary system infection contamination pathogen thalline high enrichment, and retain and be fixed on miillpore filter.This check-out console can effectively be avoided because in testing sample, pathogenic infection bacteria concentration is too low, and the false negative result caused.
2.. with the filter membrane use water diafiltration 1 time of thalline after filtration, drip 1% BSA solution 0.5ml, hatch 30 minutes, close the foreign protein of non-infection pathogen.
3.. with water diafiltration 1 time after adequate closure foreign protein, directly drip people's urinary system cause of disease bacterium infection immunity Fluorescent Diagnostic Agents 0.5ml, hatch 1 hour.Make specific immunofluorescence diagnostic reagent and the abundant specific binding of infection pathogen.
4.. with water diafiltration 3 times, fully wash away the immunofluorescence diagnostic reagent be not combined with thalline.(guaranteeing not occur false positive results) ensures miillpore filter retains the infectious bacteria thalline sample of binding specificity people urinary system cause of disease bacterium infection immunity Fluorescent Diagnostic Agents.
5.. filter membrane is placed in the retention result of observing fluorescence radiation under fluorescent microscope with relevant wavelength.Also can select other fluorescence detector, and the portable excitation light source instrument of different wave length can be excited.(as ultraviolet display etc.) carries out fluoroscopic examination to sample, judges the pathogenic microorganism thalline result of sample.
Claims (5)
1. a microbial cells microfiltration check-out console, it is characterized in that: this check-out console comprises upper strata right cylinder, handle marking plate and lower floor's hollow cylinder, described handle marking plate and lower floor's hollow cylinder or upper strata right cylinder are connected as a single entity, cylindrical lower end, upper strata is provided with external thread and forms screw bolt, the inside, upper end of lower floor's hollow cylinder is provided with internal thread and forms the screw thread screw socket coordinated with screw bolt, cylindrical centre, upper strata is provided with liquid feeding pressurization through hole, the lower end of liquid feeding pressurization through hole is provided with two-stage shoulder hole, the shoulder hole of outer end is used for setting-in filter plate with holes, inner shoulder hole forms resistance to plenum chamber, the bottom recessed formation filtered solution funnel of lower floor's hollow cylinder, the middle part of filtered solution funnel is that filtrate flow portals, for placing miillpore filter above filtered solution funnel.
2. microbial cells microfiltration check-out console according to claim 1, is characterized in that: the periphery, upper end of the filtered solution funnel bottom described lower floor's hollow cylinder is provided with a withstand voltage aprons slot of annular, is embedded with press mold cushion rubber in aprons slot.
3. microbial cells microfiltration check-out console according to claim 1 and 2, is characterized in that: described filtered solution inner wall of hopper is provided with to heart-shaped chute.
4. microbial cells microfiltration check-out console according to claim 1 and 2, is characterized in that: the plane of exterior bottom for ease of placing of described lower floor's hollow cylinder.
5. microbial cells microfiltration check-out console according to claim 1 and 2, is characterized in that: the aperture of described miillpore filter is 0.22 micron.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520091100.XU CN204462153U (en) | 2015-02-09 | 2015-02-09 | Microbial cells microfiltration check-out console |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520091100.XU CN204462153U (en) | 2015-02-09 | 2015-02-09 | Microbial cells microfiltration check-out console |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204462153U true CN204462153U (en) | 2015-07-08 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520091100.XU Expired - Fee Related CN204462153U (en) | 2015-02-09 | 2015-02-09 | Microbial cells microfiltration check-out console |
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| Country | Link |
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| CN (1) | CN204462153U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434321A (en) * | 2016-11-23 | 2017-02-22 | 百奥森（江苏）食品安全科技有限公司 | Microbial thallus microfiltration detection panel |
-
2015
- 2015-02-09 CN CN201520091100.XU patent/CN204462153U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434321A (en) * | 2016-11-23 | 2017-02-22 | 百奥森（江苏）食品安全科技有限公司 | Microbial thallus microfiltration detection panel |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150708 Termination date: 20180209 |