CN207062288U - A kind of total bacterium detecting system of water body based on simple grain sub-analysis method - Google Patents
A kind of total bacterium detecting system of water body based on simple grain sub-analysis method Download PDFInfo
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- CN207062288U CN207062288U CN201720997888.XU CN201720997888U CN207062288U CN 207062288 U CN207062288 U CN 207062288U CN 201720997888 U CN201720997888 U CN 201720997888U CN 207062288 U CN207062288 U CN 207062288U
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Abstract
The utility model provides a kind of total bacterium detecting system of water body based on simple grain sub-analysis method, including a laser, laser connection Excitation Filter with High, and the Excitation Filter with High connects laser beam shaping mirror；One light times device, fluorescent optical filter and phosphor collection mirror are connected respectively；One three-way magnetic valve, the port C and syringe pump of the three-way magnetic valve, port A connection sample bottles, port B are connected to the water inlet of capillary flow cell by flowmeter, and the capillary flow cell delivery port connects waste liquid bottle；One PCB is controlled and signal processing system, and PCB controls are connected with light times device, laser, syringe pump, double-channel solenoid valve and flowmeter respectively with signal processing system.The utility model detectable limit and sensitivity are more accurate up to a bacterial cell, testing result；Available for bacterial concentration is monitored on-line, remove the links such as artificial sample, transport, culture from, cost is greatly reduced.
Description
Technical field
A kind of water body bacterial micro-organism sum detection technique is the utility model is related to, it is especially a kind of to be analyzed based on single-particle
The total bacterium detecting system of water body of method.
Background technology
Health ministry is in GB5749-2006《Drinking water standard》Middle clear stipulaties drinking water 6 microorganisms refer to
Mark is simultaneously also in GB4789-2010《National food safety standard-food microbiological examination》In define food microorganisms and refer to
It is marked with and detection method, the method for detecting bacterium in existing national standards is mainly colony counting method, and its testing process mainly wraps
Sterile sampling, dilution culture, plate count are included, wherein sample needs to cultivate 24-48 hours in an aseptic environment, entirely detected
Journey is cumbersome, time-consuming, and testing result is lagged, it is necessary to a variety of special instruments and consumptive material.
With increasingly serious safe drinking water problem and tightened up examination criteria, traditional detection method can not expire
The current monitoring requirements of foot.Drink first water bacteria it is exceeded it is light then cause stomach disease, it is heavy then can causing death, next is drunk
Bacterial micro-organism in water has important influence, such as food and drink, medicine, microbial fermentation, state to public medical and health field
Anti- safety etc., bacterial concentration is exceeded may to directly result in research and development fail, product is unqualified etc..Water based on simple grain sub-analysis method
The total bacterium detecting system of body is cultivated without sampling, is participated in without artificial, and detecting system samples simultaneously automatically according to single-chip microcomputer setting program
It is used for quickly detecting, testing result is can be obtained by within 30 minutes, so as to realize bacterial concentration in quick detection water, and
Timely early warning, once finding there are abnormal conditions, pollution sources can be found with the very first time and block pollution.
Utility model content
In view of this, main purpose of the present utility model is to provide a kind of total bacterium inspection of water body based on simple grain sub-analysis method
Examining system.
The technical scheme that it is used is as follows：
The utility model provides a kind of total bacterium detecting system of water body based on simple grain sub-analysis method, including
One laser, laser connection Excitation Filter with High, the Excitation Filter with High connect laser beam shaping mirror；
One light times device, fluorescent optical filter and phosphor collection mirror are connected respectively；
One three-way magnetic valve, the port C and syringe pump of the three-way magnetic valve, port A connection sample bottles, port B pass through stream
Gauge is connected to the water inlet of capillary flow cell, and the capillary flow cell delivery port connects waste liquid bottle；
One PCB control and signal processing system, the PCB control with signal processing system respectively with light times device, laser, note
Penetrate pump, double-channel solenoid valve and flowmeter connection.
Further, the PCB controls include STC single-chip microcomputers, three-way magnetic valve controller, injection with signal processing system
Pump controller, light times device controller, pulse counter, memory and laser controller, the STC single-chip microcomputers respectively with threeway
Solenoid valve controller, injection pump controller, light times device controller, pulse counter, memory and laser controller connection, institute
Three-way magnetic valve controller to be stated to be connected with three-way magnetic valve, the light times device controller and pulse counter are connected with light times device,
The laser controller is connected with laser, and the injection pump controller is connected with syringe pump.
Further, the laser beam shaping mirror is combined by multiple cylindrical mirrors, and the use of each cylindrical mirror
Fused silica glass material, the laser facula after the laser shaping mirror shaping are shaped as ellipsoid, the major axis of ellipsoid hot spot
Length is approximately equal to the diameter of capillary, and ellipsoid hot spot minor axis length is less than 20 μm.
Further, the laser is 350nm-450nm band lasers.
Compared with prior art, the utility model has following beneficial effect：
1) traditional detection method is compared, the bacteria counting apparatus calculates bacterium by way of single-particle is analyzed and is directly counted
The number of cell, so as to detectable limit with sensitivity up to a bacterial cell, secondly whole detection process is all in enclosed environment
Lower automatic progress, detection process are not easy to be influenceed by artificial or peripheral environment factor, and testing result is more accurate.
2) the total number of bacteria detecting system can be used for monitoring bacterial concentration on-line, remove the rings such as artificial sample, transport, culture from
Section, so as to which monitoring cost be greatly reduced.
Brief description of the drawings
Fig. 1 is principle schematic of the present utility model.
Embodiment
The utility model is described in detail below in conjunction with accompanying drawing and specific embodiment, herein signal of the present utility model
Property embodiment and explanation be used for explaining the utility model, but be not intended as to restriction of the present utility model.
Reference picture 1, the reference of embodiment provided by the utility model are as follows：
1st, laser；2nd, laser filter；3rd, laser beam shaping mirror；4th, light times device；5th, fluorescence optical filtering；6th, fluorescence is received
Collect mirror；7th, capillary flow cell；8th, syringe pump；9th, three-way magnetic valve；10th, flowmeter；11st, sample bottle；12nd, waste liquid bottle；13、
PCB is controlled and signal processing system.
Single-particle analytic approach provided by the utility model is by particle emission fluorescence in induced with laser water, and is analyzed one by one
The method of particle fluorescence characteristic in water, realizes the detection to total number of bacteria in certain volume water, while according to total number of bacteria and water
Volume ratio close calculate water sample in bacterial concentration.
Specific scheme is as follows：
The utility model provides a kind of total bacterium detecting system of water body based on simple grain sub-analysis method, including
One laser, laser connection Excitation Filter with High, the Excitation Filter with High connect laser beam shaping mirror；
One light times device, fluorescent optical filter and phosphor collection mirror are connected respectively；
One three-way magnetic valve, the port C and syringe pump of the three-way magnetic valve, port A connection sample bottles, port B pass through stream
Gauge is connected to the water inlet of capillary flow cell, and the capillary flow cell delivery port connects waste liquid bottle；
One PCB control and signal processing system, the PCB control with signal processing system respectively with light times device, laser, note
Penetrate pump, double-channel solenoid valve and flowmeter connection.
PCB control and signal processing system include STC single-chip microcomputers, three-way magnetic valve controller, injection pump controller,
Light times device controller, pulse counter, memory and laser controller, the STC single-chip microcomputers respectively with three-way magnetic valve control
Device, injection pump controller, light times device controller, pulse counter, memory and laser controller connection processed, described three are powered
Magnetic valve controller is connected with three-way magnetic valve, and the light times device controller and pulse counter are connected with light times device, the laser
Controller is connected with laser, and the injection pump controller is connected with syringe pump.
The laser beam shaping mirror is combined by multiple cylindrical mirrors, and the use vitreous silica glass of each cylindrical mirror
Glass material, the laser facula after the laser shaping mirror shaping are shaped as ellipsoid, and the long axis length of ellipsoid hot spot is approximately equal to
The diameter of capillary, ellipsoid hot spot minor axis length are less than 20 μm.
The laser is 350nm-450nm band lasers.
The utility model additionally provides a kind of total bacterium detection method of water body based on simple grain sub-analysis method,
Comprise the following steps：
1) STC single-chip microcomputers send the instruction for opening three-way magnetic valve interface A according to the program of setting, work as solenoid valve controller
After receiving the instruction, driving three-way magnetic valve opens interface A；And STC single-chip microcomputers send according to the program of setting and extract water
The instruction of sample, after injection pump controller receives the instruction of the extraction water sample, driving syringe pump extracts water sample from sample bottle,
After the completion of syringe pump to STC single-chip microcomputers send feedback command；
2) STC single-chip microcomputers send the instruction for opening three-way magnetic valve interface B according to the program of setting, work as solenoid valve controller
After receiving the instruction, driving three-way magnetic valve opens interface B and immediately down interface A；
3) STC single-chip microcomputers send the instruction for opening laser controller and light times tube controller according to the program of setting respectively,
Laser controller opens laser switch and activation light times device respectively after receiving instruction with light times tube controller, and causes simultaneously
Flowmeter is active with pulse counter；
4) STC single-chip microcomputers according to the program of setting send detection instruction, injection pump controller receive instruction after with 1mL/min
The water sample injected in pump line is squeezed into capillary flow cell by~10mL/min flow velocity, and there is a diameter capillary flow cell center
10 μm~500 μm of capillary channel, water sample from capillary flow through when, the particle in water forms the particle beams, one by one by monitoring point,
If the particle is bacterial cell, 420nm~650nm fluorescence is launched under the irradiation of the laser after shaping, perpendicular to sharp
Fluorescence on light irradiation direction is collected by phosphor collection mirror, collimation, then filters out veiling glare by fluorescent optical filter, is then directed into
Fluorescence signal is changed into electric impulse signal by light times device, light times device, and the electric impulse signal is recorded by pulse counter, and detection is completed
Afterwards, the quantity of the pulse recorded according to pulse counter can be to obtain total number of bacteria, while according to the stream of cmf record
The volume of capillary circulation water is crossed, can be to count out bacterial concentration in the water sample.
In step 4), the fluorescence of bacterium transmitting comes from NADH and core yellow in bacterial cell
Element, NADH can launch the fluorescence of 420nm-650nm wave bands with riboflavin under the irradiation of laser.
In step 4), the phosphor collection mirror includes the fluorescence of the microcobjective and opposite side above capillary flow cell
Speculum group, fluorescent reflection microscope group include an achromatism planoconvex spotlight and a plane mirror, and fluorescent reflection microscope group can be with
By scattering to the fluorescent reflection of capillary flow cell bottom to microcobjective, microcobjective collects fluorescence and collimates fluorescence.
The capillary flow cell material is fused silica glass, and there is 10 μm -500 μm of diameter at the capillary flow cell center
Capillary, and capillary tube inner wall is by institute's chemical polishing processing.
In step 4), the pulse counter uses binary addition counter, and the system up counter is by counting
Unit and control gate cell are formed, and counting unit is then made up of the trigger with storage information function, and each is at
Electric impulse signal flip-flop number after reason, makes its counting unit increase by one on the basis of original binary digit, and protect
The numeral is held until next electric impulse signal, in a monitoring cycle, the cumulative electric pulse quantity received of counter.
The utility model can be participated in total number of bacteria in quick detection water without artificial, without cultivating sample, inspection
Examining system samples automatically according to default program, feed liquor and quick detection, testing result is gone out in 30 minutes, testing result is not stagnant
Afterwards, so as to the concentration of bacterium in quick detection water, and timely early warning, once find there are abnormal conditions, can be with the very first time
Find pollution sources and block pollution.
Claims (4)
- A kind of 1. total bacterium detecting system of water body based on simple grain sub-analysis method, it is characterised in that includingOne laser, laser connection Excitation Filter with High, the Excitation Filter with High connect laser beam shaping mirror；One light times device, fluorescent optical filter and phosphor collection mirror are connected respectively；One three-way magnetic valve, the port C and syringe pump of the three-way magnetic valve, port A connection sample bottles, port B pass through flowmeter The water inlet of capillary flow cell is connected to, the capillary flow cell delivery port connects waste liquid bottle；One PCB control and signal processing system, the PCB control with signal processing system respectively with light times device, laser, injection Pump, double-channel solenoid valve and flowmeter connection.
- 2. the total bacterium detecting system of the water body according to claim 1 based on simple grain sub-analysis method, it is characterised in that described PCB controls includes STC single-chip microcomputers, three-way magnetic valve controller, injection pump controller, the control of light times device with signal processing system Device, pulse counter, memory and laser controller, the STC single-chip microcomputers respectively with three-way magnetic valve controller, syringe pump Controller, light times device controller, pulse counter, memory and laser controller connection, the three-way magnetic valve controller It is connected with three-way magnetic valve, the light times device controller and pulse counter are connected with light times device, and the laser controller is with swashing Light device is connected, and the injection pump controller is connected with syringe pump.
- 3. the total bacterium detecting system of the water body according to claim 1 based on simple grain sub-analysis method, it is characterised in that described Laser beam shaping mirror is combined by multiple cylindrical mirrors, and the use fused silica glass material of each cylindrical mirror, is passed through Laser facula after the laser shaping mirror shaping is shaped as ellipsoid, and the long axis length of ellipsoid hot spot is approximately equal to the straight of capillary Footpath, ellipsoid hot spot minor axis length are less than 20 μm.
- 4. the total bacterium detecting system of the water body according to claim 1 based on simple grain sub-analysis method, it is characterised in that described Laser is 350nm-450nm band lasers.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201720997888.XU CN207062288U (en) | 2017-08-10 | 2017-08-10 | A kind of total bacterium detecting system of water body based on simple grain sub-analysis method |
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| CN201720997888.XU CN207062288U (en) | 2017-08-10 | 2017-08-10 | A kind of total bacterium detecting system of water body based on simple grain sub-analysis method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020001529A1 (en) * | 2018-06-27 | 2020-01-02 | 北京天天极因科技有限公司 | Light sheet fluorescence microscopic imaging device for imaging transparent droplet and test method |
| US11814619B2 (en) | 2021-06-04 | 2023-11-14 | Enumerix, Inc. | Compositions, methods, and systems for single cell barcoding and sequencing |
| US11834714B2 (en) | 2021-12-20 | 2023-12-05 | Enumerix, Inc. | Detection and digital quantitation of multiple targets |
-
2017
- 2017-08-10 CN CN201720997888.XU patent/CN207062288U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020001529A1 (en) * | 2018-06-27 | 2020-01-02 | 北京天天极因科技有限公司 | Light sheet fluorescence microscopic imaging device for imaging transparent droplet and test method |
| US11814619B2 (en) | 2021-06-04 | 2023-11-14 | Enumerix, Inc. | Compositions, methods, and systems for single cell barcoding and sequencing |
| US11834714B2 (en) | 2021-12-20 | 2023-12-05 | Enumerix, Inc. | Detection and digital quantitation of multiple targets |
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