CN206345859U - The counter of bacterial concentration in a kind of on-line monitoring water - Google Patents

The counter of bacterial concentration in a kind of on-line monitoring water Download PDF

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Publication number
CN206345859U
CN206345859U CN201621464723.8U CN201621464723U CN206345859U CN 206345859 U CN206345859 U CN 206345859U CN 201621464723 U CN201621464723 U CN 201621464723U CN 206345859 U CN206345859 U CN 206345859U
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China
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water
fluorescence
unit
laser
bottle
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CN201621464723.8U
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Chinese (zh)
Inventor
武温凯
孙国陇
刘波
越岗
严学文
田锋雪
尚存存
赵丁
杨江维
史青青
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Shaanxi Public Intelligent Science & Technology Co Ltd
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Shaanxi Public Intelligent Science & Technology Co Ltd
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Abstract

The utility model provides a kind of counter for monitoring bacterial concentration in water on-line, including compression pump, compression pump connects water-storing bottle and oil storage bottle respectively, the water-storing bottle and oil storage bottle connect the PCB integrated circuits and the droplet chip by water ga(u)ge and mineral oil flowmeter respectively, the PCB integrated circuits connect LASER Light Source respectively and vacuum light is managed again, the LASER Light Source Induction of bacterial launches fluorescence, the fluorescence and the other light sources of filter in addition to fluorescence are converged by the compound lens one and compound lens two, the fluorescence of bacterium transmitting is detected by the vacuum light times pipe, and it is anti-deaf to the PCB integrated circuits that the fluorescence is converted into electric signal, the double-tone spectroscope is arranged between compound lens one and compound lens two, for launching laser in one direction, through fluorescence, the end connection waste liquid bottle of the droplet chip.The utility model surveys process and is difficult to be influenceed by artificial or peripheral environment factor, and testing result is more accurate.

Description

The counter of bacterial concentration in a kind of on-line monitoring water
Technical field
The utility model is related to a kind of counting technology of bacterial concentration, especially a kind of to monitor bacterial concentration in water on-line Counter.
Background technology
Country's water quality monitoring system can only be monitored to the inorganic matter and organic matter of limited quantity at present, but without one Money sensor can monitor the bacterial micro-organism in water on-line.Drink that water bacteria is exceeded gently then to cause stomach disease first, weight Then can causing death, secondly the bacterial micro-organism in drinking water have important influence to public medical and health field, such as eat Drink, medicine, microbial fermentation, national defense safety etc., bacterial concentration is exceeded may to directly result in research and development fail, product is unqualified etc..
Health ministry is in GB5749~2006《Drinking water standard》Middle clear stipulaties drinking water 6 microorganisms refer to Mark is simultaneously also in GB4789~2010《National food safety standard~food microbiological examination》In define food microorganisms Method of detecting bacterium in index and detection method, existing national standards is mainly colony counting method, and its testing process is main Including sterile sampling, dilution culture, plate count, wherein sample needs to cultivate in an aseptic environment 24~48 hours, whole inspection Survey process is cumbersome, time-consuming, and testing result is delayed, it is necessary to a variety of special instruments and consumptive material.
With increasingly serious safe drinking water problem and tightened up examination criteria, traditional detection method can not expire The current monitoring requirements of foot.Bacteria counting apparatus available for bacterial concentration in on-line monitoring water is cultivated without sampling, without artificial Participate in, bacteria counting apparatus is sampled and detected automatically according to single-chip microcomputer setting program, and testing result is taken to on-line monitoring Business device.By this bacteria counting apparatus, can with the concentration of bacterium in real time on-line monitoring water, and in time early warning, once find have different Reason condition, pollution sources can be found with the very first time and pollution is blocked.
Utility model content
In view of this, main purpose of the present utility model is to provide a kind of counter for monitoring bacterial concentration in water on-line.
The technical solution adopted in the utility model is:
The counter of bacterial concentration in a kind of on-line monitoring water, including compression pump, water-storing bottle, liquid sampling device, oil storage Bottle, PCB integrated circuits, LASER Light Source, droplet chip, vacuum light are managed again, compound lens one, compound lens two, double-tone spectroscope And waste liquid bottle, the compression pump connects water-storing bottle and oil storage bottle respectively, and the water-storing bottle and oil storage bottle pass through water-carrying capacity respectively Meter and mineral oil flowmeter connect the PCB integrated circuits and the droplet chip, and the PCB integrated circuits connect laser respectively Light source and vacuum light are managed again, and the LASER Light Source Induction of bacterial launches fluorescence, is converged by the compound lens one and compound lens two Gather the fluorescence and the other light sources of filter in addition to fluorescence, the fluorescence of bacterium transmitting is detected by the vacuum light times pipe, and this is glimmering Light is converted into that electric signal is anti-deaf to the PCB integrated circuits, and the double-tone spectroscope is arranged on compound lens one and compound lens Between two, for reflection laser in one direction, through fluorescence, the end connection waste liquid bottle of the droplet chip.
It is preferred that, the droplet chip is fixed on droplet chip fixture, discrete for the water continuously flowed to be transformed into Mini-type water drop.
It is preferred that, the PCB integrated circuits include wireless communication unit, single-chip microcomputer, signal gathering unit, signal transacting list Member and pulse count unit, be provided with the single-chip microcomputer system control unit, Data Computation Unit, data storage cell with And data outputting unit, the data storage element connection data outputting unit, the wireless communication unit connection data output Unit, for the communication with Wireless Telecom Equipment, the system control unit connects signal gathering unit, the signal gathering unit Signal processing unit is connected, signal processing unit connection pulse count unit, the pulse count unit connects the data and deposited Storage unit and Data Computation Unit, on the one hand control liquid sampling device extracts 100mL to the system control unit from monitoring point Water sample injects water-storing bottle, and on the other hand triggering compression pump, LASER Light Source, mineral oil flowmeter, water ga(u)ge, vacuum light are managed again, It is active these equipment, the compression pump is after system control unit trigger signal is received respectively with 500nL/ Min~1000nL/min and 3000nL/min~4000nL/min flow velocity are by the mineral oil in the water and oil storage bottle in water-storing bottle Droplet chip is pressed into, and a diameter of 10 μm~50 μm of water-in-oil type Mini-type water drop is formed in droplet chip profit intersection, from The laser that LASER Light Source is sent is reflected by double-tone spectroscope right angle, and be then combined the convergence of lens two, the laser irradiation after convergence Flow through bacterial cell in the Mini-type water drop in detection zone, Mini-type water drop and fluorescence launched by induced with laser, bacterial fluorescence scatter to All directions, first fluorescence are combined the collimation of lens two, then through double-tone spectroscope, are imported after the lens one that are finally combined convergence Vacuum light is managed again, and fluorescence is converted into electric signal by vacuum light times pipe, and laser facula is initially entered to leaving completely from bacterial cell Laser facula, bacterium is induced continuously to launch fluorescence and its intensity shows as a light pulse, and vacuum light times pipe is by the fluorescence arteries and veins Red switch changes an electric impulse signal into, and the electric impulse signal is transfused to after signal gathering unit, signal processing unit processes Pulse count unit.
It is preferred that, the vacuum light manage again in be provided with photoelectric conversion probe and electronics flow vacuum multiplier.
It is preferred that, the droplet chip uses fused silica glass, and the droplet chip is focus type droplet chip, quarter in it Erosion has a diameter of 20 μm~200 μm of microchannel, and the microchannel wall scribbles hydrophobic layer, and the droplet chip bottom is scribbled Fluorescent reflection coating, can will scattering to the fluorescent reflection of bottom to vacuum light times pipe side.
It is preferred that, the LASER Light Source uses the laser between 340nm~380nm wave bands as induction light source.
It is preferred that, the fluorescence is the fluorescence between 450nm~550nm wave bands.
Compared with prior art, the utility model has following beneficial effect:
1) can be with the concentration of bacterium in real time on-line monitoring water, without artificial participation, without cultivating sample, bacterium Counter is sampled automatically according to default program, feed liquor and quick detection, and with inspection with result is gone out, testing result is not delayed, inspection Monitoring server is uploaded when surveying fructufy, is available for monitoring and supervisory department to download and read, while it is exceeded automatic to set up bacterial concentration Warning function, notifies supervision department to take counter-measure in time.
2) traditional detection method is compared, the bacteria counting apparatus calculates the number of bacterial cell by way of directly counting, So as to detectable limit with sensitivity up to 1 bacterial cell, secondly whole detection process is all carried out automatically under enclosed environment, is examined Survey process is difficult to be influenceed by artificial or peripheral environment factor, and testing result is more accurate.
3) bacteria counting apparatus can be used for on-line monitoring bacterial concentration, remove the links such as artificial sample, transport, culture from, and The consumptive material that the bacteria counting apparatus needs only has mineral oil, and the mineral oil can also reclaim reuse, so that monitoring is greatly reduced Cost.
Brief description of the drawings
Fig. 1 is principle schematic of the present utility model;
Fig. 2 is Fig. 1 aerial view;
Fig. 3 is droplet chip partial enlarged drawing in Fig. 1.
Wherein, the title of all parts and mark are as follows:
1st, compression pump;2nd, water-storing bottle;3rd, liquid sampling device;4th, oil storage bottle;5th, mineral oil flowmeter;6th, water ga(u)ge; 7th, PCB integrated circuits;8th, LASER Light Source;9th, droplet chip;10th, vacuum light is managed again;11st, compound lens one;12nd, double-colored light splitting Mirror;13rd, compound lens two;14th, waste liquid bottle.
Embodiment
Describe the utility model in detail below in conjunction with accompanying drawing and specific embodiment, herein signal of the present utility model Property embodiment and explanation be used for explaining the utility model, but be not intended as limiting of the present utility model.
Referring to figs. 1 to Fig. 3, the utility model discloses a kind of counter for monitoring bacterial concentration in water on-line, including pressure Power pump 1, water-storing bottle 2, liquid sampling device 3, oil storage bottle 4, PCB integrated circuits 7, LASER Light Source 8, droplet chip 9, vacuum light times Pipe 10, compound lens 1, compound lens 2 13, double-tone spectroscope 12 and waste liquid bottle 14, the compression pump 1 connect storage respectively Water bottle 2 and oil storage bottle 4, the water-storing bottle 2 and oil storage bottle 4 connect described by water ga(u)ge 6 and mineral oil flowmeter 5 respectively PCB integrated circuits 7 and the droplet chip 9, the PCB integrated circuits 7 connect LASER Light Source 8 and vacuum light times pipe 10 respectively, The LASER Light Source 8 is with using between light source Induction of bacterial transmitting 450nm~550nm wave bands between 340nm~380nm wave bands Fluorescence, converges the fluorescence and the other light sources of filter in addition to fluorescence, by institute by the compound lens 1 and compound lens 2 13 The fluorescence of the detection bacterium transmitting of vacuum light times pipe 10 is stated, and it is anti-deaf to the PCB integrated circuits that the fluorescence is converted into electric signal 7, the double-tone spectroscope 12 is arranged between compound lens 1 and compound lens 2 13, for reflecting sharp in one direction Light, through fluorescence, the end connection waste liquid bottle of the droplet chip 9.The droplet chip 9 is fixed on droplet chip fixture, The droplet chip 9 uses fused silica glass, and the droplet chip 9 is focus type droplet chip, is etched with a diameter of 20 μ in it The microchannel of m~200 μm, the microchannel wall scribbles hydrophobic layer, and the droplet chip bottom scribbles fluorescent reflection coating, Can will scattering to the fluorescent reflection of bottom to the side of vacuum light times pipe 10, it is discrete miniature for the water continuously flowed to be transformed into Water droplet,
The PCB integrated circuits 7 include wireless communication unit, single-chip microcomputer, signal gathering unit, signal processing unit and System control unit, Data Computation Unit, data storage cell and data are provided with pulse count unit, the single-chip microcomputer Output unit, the data storage element connects data outputting unit, and the wireless communication unit connects data outputting unit, uses In the communication with Wireless Telecom Equipment, the system control unit connects signal gathering unit, signal gathering unit connection letter Number processing unit, signal processing unit connection pulse count unit, the pulse count unit connects the data storage cell And Data Computation Unit, on the one hand control liquid sampling device 3 extracts 100mL water samples note to the system control unit from monitoring point Enter water-storing bottle 2, on the other hand triggering compression pump 1, LASER Light Source 8, mineral oil flowmeter 5, water ga(u)ge 6, vacuum light times pipe 10, It is active these equipment, the compression pump 1 is after system control unit trigger signal is received respectively with 500nL/ Min~1000nL/min and 3000nL/min~4000nL/min flow velocity are by the mineral in the water in water-storing bottle 2 and oil storage bottle 4 Oil pressure enters droplet chip 9, and forms a diameter of 10 μm~50 μm of water-in-oil type Miniature water in the profit intersection of droplet chip 9 Drop, the laser sent from LASER Light Source 8 is reflected by the right angle of double-tone spectroscope 12, the lens 2 13 that are then combined convergence, after convergence The bacterial cell that flows through in Mini-type water drop in detection zone, Mini-type water drop of laser irradiation fluorescence, bacterium are launched by induced with laser Fluorescent scattering is to all directions, and fluorescence first is combined the collimation of lens 2 13, then through double-tone spectroscope 12, is finally combined Mirror 1 imports vacuum light times pipe 10 after converging, and fluorescence is converted into electric signal by vacuum light times pipe 10, is entered since bacterial cell Enter laser facula to laser facula is left completely, bacterium is induced continuously to launch fluorescence and its intensity shows as a light pulse, The fluorescent pulse is converted into an electric impulse signal by vacuum light times pipe 10, and the electric impulse signal is by signal gathering unit, letter Pulse count unit is transfused to after number processing unit processes.
The utility model additionally provides a kind of method of counting of bacterial concentration, comprises the following steps:First by compression pump 1, liquid Body sampling device 3, mineral oil flowmeter 5, water ga(u)ge 6, LASER Light Source 8, vacuum light are managed be connected to PCB by controlling cable again On the one hand control liquid sampling device 3 extracts 100mL to system control unit from monitoring point in integrated circuit 7, PCB integrated circuits 7 Water sample injects water-storing bottle 2, on the other hand triggering compression pump 1, LASER Light Source 8, mineral oil flowmeter 5, water ga(u)ge 6, vacuum light Times pipe 10, is active these equipment, the compression pump 1 after system control unit trigger signal is received respectively with 500nL/min~1000nL/min and 3000nL/min~4000nL/min flow velocity is by the water in water-storing bottle 2 and oil storage bottle 4 Mineral oil press-in droplet chip 9, it is and micro- in the water-in-oil type that the profit intersection of droplet chip 9 forms a diameter of 10 μm~50 μm Type water droplet, the laser sent from LASER Light Source 8 is reflected by the right angle of double-tone spectroscope 12, the lens 2 13 that are then combined convergence, converges The bacterial cell that laser irradiation after poly- is flowed through in the Mini-type water drop in detection zone, Mini-type water drop launches fluorescence by induced with laser, Bacterial fluorescence is scattered to all directions, and fluorescence first is combined the collimation of lens 2 13, then through double-tone spectroscope 12, finally by group Close after lens 1 are converged and import vacuum light times pipe 10, fluorescence is converted into electric signal by vacuum light times pipe 10, is opened from bacterial cell Begin to enter laser facula to laser facula is left completely, bacterium is induced continuously to launch fluorescence and its intensity shows as a light arteries and veins The fluorescent pulse is converted into an electric impulse signal by punching, vacuum light times pipe 10, the electric impulse signal by signal gathering unit, Pulse count unit is transfused to after signal processing unit processes.The pulse count unit is binary addition counter, is counted Device is made up of basic counting unit and control gate cell, and counting unit is then by the trigger structure with storage information function Into each electric impulse signal flip-flop number after treatment makes its counting unit on the basis of original binary digit Upper increase by one, and the numeral is kept until next electric impulse signal, in a monitoring cycle, counter is cumulative to be received Electric pulse quantity.Electric pulse sum is transferred in PCB integrated circuits 7 by the pulse count unit in binary form Single-chip microcomputer, while counter is zeroed out, single-chip microcomputer obtains the body that the water of droplet chip 9 is flowed through in monitoring cycle from water ga(u)ge Product, and according to the overall pulse quantity of acquisition calculate unit of water body product in bacterial cell concentration, then the result be saved And export to the wireless communication unit in PCB integrated circuits 7, the wireless communication unit will be detected by cordless communication network and tied Fruit is uploaded to on-line monitoring server.
The technical scheme disclosed in the utility model embodiment is described in detail above, it is used herein specifically Embodiment is set forth to the principle and embodiment of the utility model embodiment, and the explanation of above example is only applicable to Help understands the principle of the utility model embodiment;It is real according to the utility model simultaneously for those of ordinary skill in the art Example is applied, be will change in embodiment and application, in summary, this specification content should not be understood For to limitation of the present utility model.

Claims (7)

1. a kind of counter for monitoring bacterial concentration in water on-line, it is characterised in that including compression pump, water-storing bottle, Liquid sample introduction Device, oil storage bottle, PCB integrated circuits, LASER Light Source, droplet chip, vacuum light are managed again, compound lens one, compound lens two, double Color spectroscope and waste liquid bottle, the compression pump connect water-storing bottle and oil storage bottle respectively, and the water-storing bottle and oil storage bottle lead to respectively Water flow meter and mineral oil flowmeter connect the PCB integrated circuits and the droplet chip, the PCB integrated circuits difference Connection LASER Light Source and vacuum light are managed again, and the LASER Light Source Induction of bacterial launches fluorescence, by the compound lens one and combination Lens two converge the fluorescence and the other light sources of filter in addition to fluorescence, and the fluorescence of bacterium transmitting is detected by the vacuum light times pipe, And it is anti-deaf to the PCB integrated circuits that the fluorescence is converted into electric signal, the double-tone spectroscope be arranged on compound lens one with Between compound lens two, for reflection laser in one direction, through fluorescence, the end connection waste liquid of the droplet chip Bottle.
2. the counter of bacterial concentration in on-line monitoring water according to claim 1, it is characterised in that the droplet chip It is fixed on droplet chip fixture, for the water continuously flowed to be transformed into discrete Mini-type water drop.
3. the counter of bacterial concentration in on-line monitoring water according to claim 1, it is characterised in that the PCB is integrated Circuit includes wireless communication unit, single-chip microcomputer, signal gathering unit, signal processing unit and pulse count unit, the list System control unit, Data Computation Unit, data storage cell and data outputting unit, the data storage are provided with piece machine Memory cell connects data outputting unit, and the wireless communication unit connects data outputting unit, for Wireless Telecom Equipment Communication, the system control unit connects signal gathering unit, signal gathering unit connection signal processing unit, signal transacting Unit connects pulse count unit, and the pulse count unit connects the data storage cell and Data Computation Unit, described On the one hand control liquid sampling device extracts 100mL water samples injection water-storing bottle to system control unit from monitoring point, on the other hand touches Hair compression pump, LASER Light Source, mineral oil flowmeter, water ga(u)ge, vacuum light are managed again, are active these equipment, institute Compression pump is stated after system control unit trigger signal is received respectively with 500nL/min~1000nL/min and 3000nL/min Mineral oil in water and oil storage bottle in water-storing bottle is pressed into droplet chip by~4000nL/min flow velocity, and in droplet chip oil Water intersection forms a diameter of 10 μm~50 μm of water-in-oil type Mini-type water drop, and the laser sent from LASER Light Source is by double-colored light splitting Mirror right angle reflects, and be then combined the convergence of lens two, and the laser irradiation after convergence flows through the Mini-type water drop in detection zone, Miniature water Bacterial cell in drop launches fluorescence by induced with laser, and bacterial fluorescence is scattered to all directions, and fluorescence first is combined lens two Collimation, then through double-tone spectroscope, importing vacuum light is managed again after the lens one that are finally combined convergence, and vacuum light times pipe turns fluorescence Change electric signal into, initially enter laser facula from bacterial cell is induced continuously to launch glimmering to laser facula, bacterium is left completely Light and its intensity shows as a light pulse, the fluorescent pulse is converted into an electric impulse signal by vacuum light times pipe, the electric arteries and veins Rush signal and be transfused to pulse count unit after signal gathering unit, signal processing unit processes.
4. the counter of bacterial concentration in the on-line monitoring water according to claim 1 or 3, it is characterised in that the vacuum Light is provided with photoelectric conversion probe and electronics flow vacuum multiplier in managing again.
5. the counter of bacterial concentration in the on-line monitoring water according to claim 1 or 2 or 3, it is characterised in that described micro- Drop chip uses fused silica glass, and the droplet chip is focus type droplet chip, is etched with a diameter of 20 μm~200 μm in it Microchannel, the microchannel wall scribbles hydrophobic layer, and the droplet chip bottom scribbles fluorescent reflection coating, will can scatter To the fluorescent reflection of bottom to vacuum light times pipe side.
6. the counter of bacterial concentration in the on-line monitoring water according to claim 1 or 3, it is characterised in that the laser Light source uses the laser between 340nm~380nm wave bands as induction light source.
7. the counter of bacterial concentration in the on-line monitoring water according to claim 1 or 3, it is characterised in that the fluorescence For the fluorescence between 450nm~550nm wave bands.
CN201621464723.8U 2016-12-29 2016-12-29 The counter of bacterial concentration in a kind of on-line monitoring water Expired - Fee Related CN206345859U (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108251290A (en) * 2016-12-29 2018-07-06 陕西公众智能科技有限公司 The counter and method of counting of bacterial concentration in a kind of on-line monitoring water
CN109316866A (en) * 2018-11-28 2019-02-12 史青青 A kind of flue gas purification device that rubber production apparatus has surplus heat using function
CN110174382A (en) * 2019-05-23 2019-08-27 安徽华米生物科技有限公司 It is a kind of for detecting the device of sewage bacterial concentration
CN113388488A (en) * 2020-03-12 2021-09-14 广州中国科学院先进技术研究所 Probe type biomass on-line detection device capable of achieving high-temperature sterilization

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108251290A (en) * 2016-12-29 2018-07-06 陕西公众智能科技有限公司 The counter and method of counting of bacterial concentration in a kind of on-line monitoring water
CN109316866A (en) * 2018-11-28 2019-02-12 史青青 A kind of flue gas purification device that rubber production apparatus has surplus heat using function
CN110174382A (en) * 2019-05-23 2019-08-27 安徽华米生物科技有限公司 It is a kind of for detecting the device of sewage bacterial concentration
CN110174382B (en) * 2019-05-23 2020-11-20 安徽维嵩生产力促进有限公司 Device for detecting sewage bacteria concentration
CN113388488A (en) * 2020-03-12 2021-09-14 广州中国科学院先进技术研究所 Probe type biomass on-line detection device capable of achieving high-temperature sterilization
CN113388488B (en) * 2020-03-12 2023-09-29 迪必尔智能科技(深圳)有限公司 Probe type biomass on-line detection device capable of achieving high-temperature sterilization

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